| a structured dynamic model for the baculovirus infection process in insect-cell reactor configurations. | a mathematical description of the infection of insect cells with baculovirus in a continuously operated reactor configuration is presented. the reactor configuration consists of one bioreactor in which insect cells (spodoptera frugiperda) are grown followed by one or two bioreactors in which cells are infected by a baculovirus (autographa californica nuclear polyhedrosis virus). it was demonstrated that the so-called passage effect is responsible for the observed difference in run time between a ... | 1992 | 18601149 |
| culture of insect cells in helical ribbon impeller bioreactor. | an 11-l helical ribbon impeller (hri) bioreactor was tested for the culture of spodoptera frugiperda (sf-9) cells. this impeller and surface baffling ensured homogeneous mixing and high oxygen transfer through surface aeration and surface-induced babble generation. serum-supplemented and serum-free cultures, using tnmfh and ipl/41 media, respectively, grew a similar specific growth rates(0.031 and 0.028 h(-1)) to maximum cell densities of 5.5 x 10(6)-6.0 x 10(6) cells. ml(-1) with viability exce ... | 1991 | 18604880 |
| production of recombinant proteins in high-density insect cell cultures. | the effect of the growth phase of spodoptera frugiperda (sf9) cells on the production of recombinant proteins (beta-galactosidase and glucocerebrosidase) was investigated. cells infected with the recombinant autographa californica nuclear polyhedrosis virus at the late exponential and stationary phases yielded low quantities of expressed protein. highest enzyme yields were obtained using sf9 cells from the early exponential phase (0.9 mg beta-galactosidase/10(6) cells and 1.7 microg glucocerebro ... | 1993 | 18612984 |
| utilization of glucose and amino acids in insect cell cultures: quantifying the metabolic flows within the primary pathways and medium development. | the current understanding of insect cell metabolism is very limited. in order to gain some insight into the growth and metabolism of insect cells spodoptera frugiperda (sf9), a comprehensive characterization of culture conditions for cells grown in the ipl-41 medium was made by measuring the amino acid composition of the growth medium and the cell extract, the macromolecular composition of the cells (dna, rna, and protein), medium concentrations of various metabolites and sugars, and the evolved ... | 1993 | 18613102 |
| quantification of damage to suspended insect cells as a result of bubble rupture. | it is proposed that when cells are either attached to, or very near, a rupturing bubble, the hydrodynamic forces associated with the rupture are sufficient to kill the cells. four types of experiments were conducted to quantify the number and location of these killed cells. we determined: (1) the number of cells killed as a result of a single, 3.5-mm bubble rupture; (2) the number and viability of cells in the upward jet that results when a bubble ruptures; (3) the number of cells on the bubble ... | 1994 | 18613308 |
| using haplotypes to monitor the migration of fall armyworm (lepidoptera: noctuidae) corn-strain populations from texas and florida. | fall armyworm, spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae), infestations in most of north america north of mexico arise from annual migrations of populations that overwinter in southern texas and florida. a comparison of the cytochrome oxidase i haplotype profiles within the fall armyworm corn-strain, the subgroup that preferentially infests corn (zea mays l.) and sorghum (sorghum vulgare pers.), identified significant differences in the proportions of certain haplotypes between ... | 2008 | 18613574 |
| characterization of anopheles minimus cyp6aa3 expressed in a recombinant baculovirus system. | metabolism by cytochrome p450 monooxygenases is a major mechanism implicated in resistance of insects to insecticides, including pyrethroids. we previously isolated the cytochrome p450 cyp6aa3 from deltamethrin-selected resistant strain of anopheles minimus mosquito, a major malaria vector in thailand. in the present study, we further investigated the role of cyp6aa3 enzyme in deltamethrin metabolism in vitro. the cyp6aa3 was expressed in spodoptera frugiperda (sf9) insect cells via baculovirus- ... | 2008 | 18615616 |
| baculovirus expression system scaleup by perfusion of high-density sf-9 cell cultures. | a perfusion system based on a 4-l stirred tank bioreactor and a custom-designed tangential (cross-flow) filter was assembled to realize a scaleup of the baculovirus expression vector system (bevs). when perfused with 1 to 1.5 vol/day, spodoptera frugiperda (sf-9) insect cell cultures grew from 4 x 10(6) to 15 x 10(6) cells/ml over 3 to 4 days. the possibility of maintaining high specific production of recombinant vp6 protein (from bovine rotavirus) after baculovirus infection of the high-density ... | 1994 | 18615881 |
| "oryctes" virus replication: electron microscopic observations on infected moth and mosquito cells. | "oryctes" virus replicates efficiently in continuous moth (spodoptera frugiperda) and mosquito (aedes albopictus) cell cultures. progeny virus was detected by bioassay and electron microscopy 15 hr after infection in both cell cultures. s. frugiperda cells permitted replication morphologically similar to that observed in vivo occurring in the cell nucleus. a. albopictus cells permitted replication of the virus solely in the cytoplasm. | 1976 | 18621357 |
| the effect of oscillating dissolved oxygen concentrations on the metabolism of a spodoptera frugiperda iplb-sf21-ae clonal isolate. | the effect of oscillating dissolved oxygen (do) concentration on the metabolism of a clonal isolate of the spodoptera frugiperda iplb-sf21-ae insect cell line was investigated. specifically, the effect on cell growth, re- combinant protein synthesis, glucose and glutamine consumption, and lactate accumulation was determined. prior to conducting the oscillating do experiments, it was found that the do concentration could be reduced to 15% air saturation without adversely affecting the growth rate ... | 1995 | 18623445 |
| on-line monitoring of respiration in recombinant-baculovirus infected and uninfected insect cell bioreactor cultures. | respiration rates in spodoptera frugiperda (sf-9) cell bioreactor cultures were successfully measured on-line using two methods: the o(2) uptake rate (our) was determined using gas phase po(2) values imposed by a dissolved oxygen controller and the co(2) evolution rate (cer) was measured using an infrared detector. the measurement methods were accurate, reliable, and relatively inexpensive. the cer was routinely determined in bioreactor cultures used for the production of several recombinant pro ... | 1996 | 18626897 |
| on the origin of the polyhedral protein of autographa californica nuclear polyhedrosis virus. isolation, characterization, and translation of viral messenger rna. | virus-specific messenger rna was isolated 24 hr postinfection from spodoptera frugiperda cells infected with autographa californica nuclear polyhedrosis virus (acnpv) by means of hybridization with viral dna that was coupled to cellulose. viral mrna was analyzed on isokinetic sucrose gradients and on polyacrylamide gels containing 98% formamide. viral mrna was also translated in an in vitro protein-synthesizing system derived from wheat germ. one product comigrated with purified acnpv polyhedral ... | 1980 | 18631636 |
| autographa californica nuclear polyhedrosis virus-induced proteins in tissue culture. | the multiplication of autographa californica nuclear polyhedrosis virus was studied in trichoplusia ni and spodoptera frugiperda insect cell lines. using pulse labeling, 18 viral-induced proteins were identified by polyacrylamide gel electrophoresis. based on infectivity curves and protein synthesis studies, replication could be divided into four phases. during the early phase of infection, four viral-induced proteins with molecular weights of 45, 35, 34, and 31 thousand daltons could be detecte ... | 1980 | 18631644 |
| radioimmunoassay analysis of baculovirus granulins and polyhedrins. | granulin and polyhedrin proteins were purified by preparative sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis from the baculoviruses autographa californica, rachiplusia ou, heliothis zea, heliothis armigera, trichoplusia ni, and spodoptera frugiperda. antisera were raised against autographa californica (ac) polyhedrin and trichoplusia ni (tn) granulin and analyzed for homologous and heterologous immunoreactivity by immunodiffusion and radioimmunoassay (ria). ac polyhedrin and tn ... | 1980 | 18631650 |
| effect of elevated oxygen and glutamine levels on foreign protein production at high cell densities using the insect cell-baculovirus expression system. | per cell protein expression in virally-infected insect cells declines significantly at high cell density resulting in a decrease in volumetric productivity. specific protein expression levels in spodoptera frugiperda (sf-21) cells could be increased at high cell densities by increasing the oxygen supply and by supplementing the medium with glutamine post-infection. beta-galactosidase yield was increased from 411 to 855 iu/ml by increasing the glutamine concentration in the medium by 46% and incr ... | 1997 | 18634082 |
| induction of a nonoccluded baculovirus persistently infecting heliothis zea cells by heliothis armigera and trichoplusia ni nuclear polyhedrosis viruses. | a nonoccluded singly enveloped baculovirus (baculovirus x) persistently infects heliothis zea (imc-hz-1) cells in culture. singly enveloped nuclear polyhedrosis viruses from h. zea and heliothis armigera, and multiply enveloped nuclear polyhedrosis viruses from trichoplusia ni, spodoptera frugiperda, and spodoptera littoralis were all found to induce baculovirus x. experiments are reported which use metabolic inhibitors and inactivated inducing virus to show that it is probable that a structural ... | 1981 | 18635066 |
| investigation of genetic heterogeneity in wild isolates of spodoptera frugiperda nuclear polyhedrosis virus by restriction endonuclease analysis of plaque-purified variants. | spodoptera frugiperda nuclear polyhedrosis virus (sfmnpv was obtained from four different sources and each isolate was cultured in iplb-sf21 cells. viral dna from each isolate was analyzed with ecor1, xho1, and bamh1 restriction endonucleases. all four isolates could be distinguished on the basis of minor differences in ecor1 patterns. submolar dna restriction fragments were seen in all isolates indicating that each isolate consisted of a mixture of different genotypes. to investigate this, two ... | 1981 | 18635067 |
| attempts to obtain oryctes baculovirus replication in three insect cell cultures. | attempts were made to obtain oryctes baculovirus replication in three insect cell cultures. the cell cultures were derived from the dynastine beetle heteronychus arator, the fall armyworm spodoptera frugiperda, and the mosquito aedes albopictus. the criteria for virus replication were synthesis of virus-specific dna and presence of virus particles within cells. infection occurred in h. arator cells but not in s. frugiperda cells. when a. albopictus cells were inoculated with high concentrations ... | 1981 | 18635075 |
| expression of cell surface gpi-anchored human p97 in baculovirus-infected insect cells. | the baculovirus/insect cell system (autographa californica multiple nuclear polyhedrosis virus/spodoptera frugiperda sf9 cells) was used to express the gpi-anchored human melanoma tumor antigen, melanotransferrin or p97. this system served to study the expression and productivity of recombinant gpi-anchored p97 by insect cells. the sf9 cells expressed a cell surface gpi-anchored form of p97 as well as a soluble form of p97 that did not appear to be derived from the gpi-anchored form of p97. both ... | 1997 | 18636443 |
| comparison of trichoplusia ni bti-tn-5b1-4 (high five) and spodoptera frugiperda sf-9 insect cell line metabolism in suspension cultures. | nutrient utilization and byproduct accumulation were monitored in spodoptera frugiperda sf-9 and trichoplusia ni bti-tn-5b1-4 (high five) cell lines during growth and following viral infection in suspension cultures in order to develop a better understanding of cell metabolism and to acquire information relevant to large scale fed-batch bioreactors. the utilization of glucose, dissolved oxygen, and amino acids were monitored in sf-9 cell cultures grown in sf-900 ii serum-free medium (sfm) and in ... | 1997 | 18636600 |
| substrate limitation in the baculovirus expression vector system. | the inability to infect insect cell cultures at the highest achievable cell densities has imposed major limitations to both the fundamental understanding of the baculovirus expression vector system (bevs) as well as full exploitation of its potential productive capacity for recombinant (beta-galacnpv) products. the current literature does not characterize and identify the exact nature of the observed limitations, which therefore has become the major objective and contribution of the following st ... | 1997 | 18636607 |
| characterization of two abundant mrnas of autographa californica nuclear polyhedrosis virus present late in infection. | cytoplasmic poly(a)(+) rna isolated from spodoptera frugiperda cells late after infection with autographa californica nuclear polyhedrosis virus (30-40 hr pi) was fractionated according to size on denaturing methyl mercury gels. two major rna species (1.4 kb and 0.75 kb) and several minor rna species were detected by ethidium bromide staining. the predominant rna species of about 1.4 kb was considered to be polyhedrin mrna because (1) in vitro translation of the rna, which was eluted from methyl ... | 1983 | 18638868 |
| serological analysis of 17 baculoviruses from subgroups a and b using protein blot immunoassay. | we used the protein blot radioimmunoassay technique (smith and summers, 1981) to evaluate the immunoreactivity of antisera made against sds-disrupted baculovirus structural proteins. eight antisera (six for npvs and two for gvs) were tested against eleven nuclear polyhedrosis (npv) and six granulosis viruses (gv). homologous reactions revealed that sds-disrupted baculovirus antigens elicited antibodies which reacted with several of the virus proteins. heterologous reactions of each viral antiser ... | 1983 | 18638912 |
| mapping early transcription products of autographa californica nuclear polyhedrosis virus. | the regions of the acmnpv genome represented as cytoplasmic transcripts early after infection of spodoptera frugiperda cells prior to and encompassing the initiation of dna replication were mapped. in vivo(32)p pulse-labeled cytoplasmic rna from infected cells at various times postinfection was used to probe southern blots of cloned ecori acmnpv dna fragments. at the earliest time point studied (0.5 - 2.5 hr p.i.) transcripts represented a large proportion of the genome although specific regions ... | 1983 | 18638940 |
| a semipermissive nuclear polyhedrosis virus infection: characterization of infection kinetics and morphogenesis. | an in vitro host-range model system, designated system ii, was developed for the multicapsid nuclear polyhedrosis virus spodoptera frugiperda (sf-mnpv). this system consisted of sf-mnpv-infected s. frugiperda cells (permissive system) and sf-mnpv-infected trichoplusia ni cells (semipermissive system). infection kinetic studies revealed that while progeny virus was produced in the semipermissive system, the infectious virus yield was at least 700-fold lower than that in positive controls. electro ... | 1983 | 18639138 |
| a study of the glycoproteins of autographa californica nuclear polyhedrosis virus (acnpv). | pulse labeling with tritiated mannose was used to follow the time course of autographa californica nuclear polyhedrosis virus (acnpv) glycoprotein synthesis in spodoptera frugiperda iplb-21 cells. nine viral-induced intracellular glycoproteins were first detected from as early as 2 hr postinoculation (67k, early phase) to as late as 14 hr (36k and 19k glycoproteins, intermediate phase). glycosylation of these proteins was observed to continue to the end of the experiment (28 hr postinoculation). ... | 1983 | 18639173 |
| translational enhancement of recombinant protein synthesis in transgenic silkworms by a 5'-untranslated region of polyhedrin gene of bombyx mori nucleopolyhedrovirus. | previously, we established a method to produce recombinant proteins (r-proteins) in cocoons of germline transgenic silkworms, and showed that a step(s) in post-transcription processes was rate-limiting in obtaining a high yield of r-proteins. in this study, we examined whether the 5'-untranslated region (5'-utr) of the polyhedrin gene (pol) of nucleopolyhedrovirus (npv) has a translational enhancer activity in the r-protein expression by middle silk gland (msg) cells of silkworm bombyx mori (bm) ... | 2008 | 18640598 |
| application technology for the entomopathogenic nematodes heterorhabditis indica and steinernema sp. (rhabditida: heterorhabditidae and steinernematidae) to control spodoptera frugiperda (smith) (lepidoptera: noctuidae) in corn. | the effects of different application technologies were evaluated on the concentration, viability, and efficiency of infective juveniles of the nematodes heterorhabditis indica poinar, karunakar & david and steinernema sp. (ibcb-n6) to control spodoptera frugiperda smith on corn plants. two hundred and eighty infective juveniles of steinernema sp. were required to kill 100% third-instar fall armyworms in petri dishes, as compared to 400 infective juveniles of the h. indica nematode to obtain 75% ... | 2008 | 18641902 |
| spatial analysis of the distribution of spodoptera frugiperda (j.e. smith) (lepidoptera: noctuidae) and losses in maize crop productivity using geostatistics. | the fall armyworm, spodoptera frugiperda (j.e. smith), is one of the chief pests of maize in the americas. the study of its spatial distribution is fundamental for designing correct control strategies, improving sampling methods, determining actual and potential crop losses, and adopting precise agricultural techniques. in são paulo state, brazil, a maize field was sampled at weekly intervals, from germination through harvest, for caterpillar densities, using quadrates. in each of 200 quadrates, ... | 2008 | 18641904 |
| bcl-2 expression in spodoptera frugiperda sf-9 and trichoplusia ni bti-tn-5b1-4 insect cells: effect on recombinant protein expression and cell viability. | the effect of bcl-2 expression on cell viability and recombinant protein synthesis was investigated in the spodoptera frugiperda sf-9 and trichoplusia ni bti-tn-5b1-4 (high fivetrade mark) insect cell lines. it was found that coinfection with a baculovirus expressing bcl-2 [autographa californica nuclear polyhedrosis virus (acnpv)-bcl2] extended the life span of high fivetrade mark cells but not sf-9 cells when compared to infection with recombinant baculoviruses expressing either human tissue p ... | 1997 | 18642241 |
| hahb4, a sunflower hd-zip protein, integrates signals from the jasmonic acid and ethylene pathways during wounding and biotic stress responses. | the helianthus annuus (sunflower) hahb4 transcription factor belongs to the hd-zip family and its transcript levels are strongly induced when sunflower plants are attacked by herbivores, mechanically damaged or treated with methyl-jasmonic acid (meja) or ethylene (et). promoter fusion analysis, in arabidopsis and in sunflower, demonstrated that induction of hahb4 expression by these treatments is regulated at the transcriptional level. in transiently transformed sunflower plants hahb4 expression ... | 2008 | 18643970 |
| single cell analysis in full body quartz glass chips with native uv laser-induced fluorescence detection. | in order to investigate the individual and inhomogenous cellular response, e.g. to external stimuli, single cell analysis is mandatory and may provide new cognitions in proteomics as well as in other fields of systems biology in the future. here, we report on novel chip architectures for single cell analysis based on full body quartz glass microfluidic chips (qg chips) that extend our previous studies in polydimethylsiloxane (pdms) chips, and enhance the detection sensitivity of native uv laser- ... | 2008 | 18657818 |
| kinetic analysis of l1 homophilic interaction: role of the first four immunoglobulin domains and implications on binding mechanism. | l1 is a cell adhesion molecule of the immunoglobulin (ig) superfamily, critical for central nervous system development, and involved in several neuronal biological events. it is a type i membrane glycoprotein. the l1 ectodomain, composed of six ig-like and five fibronectin (fn) type-iii domains, is involved in homophilic binding. here, co-immunoprecipitation studies between recombinant truncated forms of human l1 expressed and purified from insect spodoptera frugiperda sf9 cells, and endogenous ... | 2008 | 18701456 |
| h5n1 vlp vaccine induced protection in ferrets against lethal challenge with highly pathogenic h5n1 influenza viruses. | in this study, recombinant virus-like particles (vlps) were evaluated as a candidate vaccine against emerging influenza viruses with pandemic potential. the vlps are composed of the hemagglutinin (ha), neuraminidase (na), and matrix 1 (m1) proteins of the h5n1 a/indonesia/05/2005 (clade 2.1; [indo/05]) virus, which were expressed using baculovirus in spodoptera frugiperda (sf9) cells. ferrets received either 2 injections of the vlp vaccine at escalating doses (based on ha content), recombinant h ... | 2008 | 18706956 |
| g protein beta(1)gamma (2) subunits purification and their interaction with adenylyl cyclase. | a preliminary study on the interaction of g protein (guanine triphosphate binding protein) beta(1)gamma(2) subunits and their coupled components in cell signal transduction was conducted in vitro. the insect cell lines, sf9 (spodoptera frugiperda) and h5 (trichoplusia ni) were used to express the recombinant protein gbeta(1)gamma(2). the cell membrane containing gbeta(1)gamma(2) was isolated through affinity chromatography column with ni-ntaagarose by fplc method, and the highly purified protein ... | 2003 | 18758712 |
| peritrophic membrane role in enhancing digestive efficiency. theoretical and experimental models. | the peritrophic membrane (pm) is an anatomical structure surrounding the food bolus in most insects. rejecting the idea that pm has evolved from coating mucus to play the same protective role as it, novel functions were proposed and experimentally tested. the theoretical principles underlying the digestive enzyme recycling mechanism were described and used to develop an algorithm to calculate enzyme distributions along the midgut and to infer secretory and absorptive sites. the activity of a spo ... | 2008 | 18761346 |
| neuropeptides associated with the regulation of feeding in insects. | the stomatogastric nervous system plays a pivotal role in feeding behaviour. central to this system is the frontal ganglion, which is responsible for foregut motor activity, and hence the passage of food through the gut. many insect peptides, which exhibit myoactivity on the visceral muscles of the gut in vitro, have been detected in the stomatogastric nervous system by immunochemical or mass spectrometric techniques. this localisation of myoactive peptides, particularly in the frontal ganglion, ... | 2009 | 18775723 |
| gene expression of a novel defensin antimicrobial peptide in the silkworm, bombyx mori. | cdna encoding a novel defensin (bmdefensinb) was cloned from the fat body of the silkworm, bombyx mori, and gene expression was analyzed. bmdefensinb showed typical structural characteristics of invertebrate defensins. phylogenetic and bootstrap analyses indicated that it has no orthologs, whereas previously reported bmdefensina is the ortholog of spodoptera frugiperda (sf)spodoptericin. the bmdefensinb gene was expressed tissue-specifically in the fat body and was strongly activated by bacteria ... | 2008 | 18776686 |
| insecticidal crystal proteins from native bacillus thuringiensis: numerical analysis and biological activity against spodoptera frugiperda. | fourteen strains of bacillus thuringiensis collected from both larvae showing disease symptoms and soil samples in northwest argentina were characterized by insecticidal activity against spodoptera frugiperda. first instar larvae and protein profile sds-page analysis of whole cell proteins not only allowed the differentiation of native bacillus thuringiensis but also revealed the possibility of applying protein profile analysis in classification of toxicity patterns. cluster analysis showed that ... | 2009 | 18800190 |
| detection and identification of baculovirus pesticides by multitemperature single-strand conformational polymorphism. | the method of single-strand conformational polymorphism (sscp) was modified in our laboratories for the characterization of baculoviruses, insect viruses with great potential for use as bioinsecticides in biological protection programs. a series of primers were synthesized after the comparison of the polyhedrin gene sequences of over 20 baculoviruses. polyhedrin is a highly conserved protein which is responsible for the persistence of the virus in the environment. universal primers were designed ... | 2008 | 18803107 |
| probing inhibitory effects of antrodia camphorata isolates using insect cell-based impedance spectroscopy: inhibition vs chemical structure. | a continuous online technique based on electric cell-substrate impedance sensing (ecis) was used for probing inhibitory effects on spodoptera frugiperda sf9 insect cells exposed to structurally similar compounds isolated and purified from the fruiting bodies of the fungus antrodia camphorata. such chemicals consisted of three ergostane-related steroids and five lanosta-related triterpenes, which are known for their diverse properties and use in the formulation of nutraceuticals and functional fo ... | 2008 | 18816072 |
| development of a novel preparation method of recombinant proteoliposomes using baculovirus gene expression systems. | we have developed a novel method for the preparation of 'recombinant proteoliposomes'. membrane proteins were expressed on budded virus (bv) envelopes using baculovirus gene expression systems, and proteoliposomes were prepared by fusion of these viruses with liposomes. first, plasmid dna containing the gene for the thyroid-stimulating hormone receptor (tshr) or the acetylcholine receptor alpha-subunit (achralpha) was co-transfected with wild type virus [autographa californica nuclear polyhedros ... | 2008 | 18836200 |
| the effect of vibrio alginolyticus infection on caspase-3 expression and activity in white shrimp litopenaeus vannamei. | complementary (c)dna encoding cysteine aspartate protease-3 (caspase-3) messenger (m)rna of the white shrimp litopenaeus vannamei was obtained from haemocytes by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race) using oligonucleotide primers based on the caspase sequences of penaeus merguiensis, spodoptera frugiperda, spodoptera littoralis, and penaeus monodon. the 1212-bp cdna contained an open reading frame (orf) of 951bp, a 56-bp 5'-untrans ... | 2008 | 18840531 |
| autographa californica multiple nucleopolyhedrovirus 38k is a novel nucleocapsid protein that interacts with vp1054, vp39, vp80, and itself. | it has been shown that the autographa californica multiple nucleopolyhedrovirus (acmnpv) 38k (ac98) is required for nucleocapsid assembly. however, the exact role of 38k in nucleocapsid assembly remains unknown. in the present study, we investigated the relationship between 38k and the nucleocapsid. western blotting using polyclonal antibodies raised against 38k revealed that 38k was expressed in the late phase of infection in acmnpv-infected spodoptera frugiperda cells and copurified with budde ... | 2008 | 18922869 |
| baculovirus production for gene therapy: the role of cell density, multiplicity of infection and medium exchange. | one of the major concerns regarding the use of insect cells and baculovirus expression vectors for the production of recombinant proteins is the drop in production observed when infecting cultures at high cell densities; this work attempts to understand this so-called cell density effect in the scope of baculovirus production for gene therapy purposes. a spodoptera frugiperda insect cell line (sf-9) was cultured and infected in serum-free medium, and the patterns of production of a recombinant b ... | 2009 | 18923829 |
| recombinant expression of aryl hydrocarbon receptor for quantitative ligand-binding analysis. | recombinant expression of the aryl hydrocarbon receptor (ahr) yields small amounts of ligand-binding-competent ahr. therefore, spodoptera frugiperda (sf9) cells and baculovirus have been evaluated for high-level and functional expression of ahr. rat and human ahr were expressed as soluble protein in significant amounts. expression of ligand-binding-competent ahr was sensitive to the protein concentration of sf9 extract, and coexpression of the chaperone p23 failed to affect the yield of function ... | 2009 | 18938125 |
| aphid acquisition and cellular transport of potato leafroll virus-like particles lacking p5 readthrough protein. | abstract lepidopteran cells (spodoptera frugiperda) produced isometric virus-like particles (vlp) when infected with a recombinant baculovirus ac61 that contained the potato leafroll virus (plrv) coat protein gene modified with an n-terminal histidine tag (p3-6h). cells infected with acfl, a recombinant baculovirus that expressed cdna copies of the plrv genome rna, did not produce virus-like particles (vlp). in cell lines doubly infected with ac61 and acfl, vlp were formed that contained plrv-rn ... | 2000 | 18944480 |
| transactivator ie1 is required for baculovirus early replication events that trigger apoptosis in permissive and nonpermissive cells. | immediate early viral protein ie1 is a potent transcriptional activator encoded by baculoviruses. although the requirement of ie1 for multiplication of autographa californica multicapsid nucleopolyhedrovirus (acmnpv) is well established, the functional roles of ie1 during infection are unclear. here, we used rna interference to ablate ie1, plus its splice variant ie0, and thereby define in vivo activities of these early proteins, including gene-specific regulation and induction of host cell apop ... | 2009 | 18945761 |
| nonviral production of human interleukin-7 in spodoptera frugiperda insect cells as a soluble recombinant protein. | human interleukin-7 (hil-7) is a cytokine secreted by the stromal cells of the red marrow. it is important for proliferation during certain stages of b-cell maturation and for t and nk cell survival, development, and homeostasis. it is a critical growth factor for enhancement and recovery of the immune t-cell. because of its strong immunomodulatory effects, hil-7 may become a valuable supplementary agent for immunotherapeutical treatments in patients with hiv infection or immunodeficiency. human ... | 2009 | 18949045 |
| a recombinant west nile virus envelope protein vaccine candidate produced in spodoptera frugiperda expressf+ cells. | in this study, a recombinant truncated west nile virus envelope protein antigen (rwnv-e) was produced in serum-free cultures of the expressf+ insect cell line via baculovirus infection. this production system was selected based on its use in the production of candidate human and animal vaccine antigens. a defined fermentation and purification process for the rwnv-e antigen was established to control for purity and immunogenicity of each protein batch. the material formulated with aluminum hydrox ... | 2009 | 18996430 |
| type 1 ribosome-inactivating proteins - entomotoxic, oxidative and genotoxic action on anticarsia gemmatalis (hübner) and spodoptera frugiperda (j.e. smith) (lepidoptera: noctuidae). | ribosome-inactivating proteins (rips) from plants inhibit protein synthesis by inactivating ribosomes. some two-chain (type 2) rips are highly toxic and may play a role in plant defense. the lower toxicity of single-chain (type 1) rips reflects the lack of a protein domain able to bind to, and translocate the toxin across cell membranes. we studied the effect of single-chain rips, lychnin, momordin, gelonin, pap-s and saporin s-6, in larvae of anticarsia gemmatalis and spodoptera frugiperda. aft ... | 2009 | 19000694 |
| expression of a spider venom peptide in transgenic tobacco confers insect resistance. | spider venom contains a mixture of peptide toxins, some able to kill insects specifically to those considered as important pest. in this study, a peptide toxin produced by the macrothele gigas spider, magi 6, was cloned and expressed in tobacco plants, as this toxin has been shown to constitute an effective insecticide. for this purpose, a genetic construction for the cdna that codifies for magi 6 was subcloned in a plant expression vector using the 35s promoter and the 5'-end leader from tobacc ... | 2009 | 19000914 |
| transfection of insect cell lines using polyethylenimine. | insect cell lines have been widely used in recombinant baculovirus expression systems and transient gene expression studies. critical to these applications have been the transfection of foreign dna. this has been frequently done using labor intensive and cytotoxic liposome-based transfection reagents. in the current study we have optimized a new kind of polyethylenimine-based dna transfection reagent on the spodoptera frugiperda sf9 insect cell line. a plasmid vector that transiently expresses g ... | 2006 | 19002899 |
| growth, viral production and metabolism of a helicoverpa zea cell line in serum-free culture. | insect cell cultures have been extensively utilised for means of production for heterologous proteins and biopesticides. spodoptera frugiperda (sf9) and trichoplusia ni (high fivetrade mark) cell lines have been widely used for the production of recombinant proteins, thus metabolism of these cell lines have been investigated thoroughly over recent years. the helicoverpa zea cell line has potential use for the production of a biopesticide, specifically the helicoverpa armigera single-nucleocapsid ... | 2003 | 19002933 |
| cloning and expression of the hn gene from the velogenic viscerotropic newcastle disease virus strain af2240 in sf9 insect cells. | the haemagglutinin-neuraminidase (hn) gene ofnewcastle disease virus (ndv) strain af2240, amplifiedfrom the viral genomic rna ( approximately 1.8 kb) was directionallycloned and inserted into a baculovirus expressionvector system. the recombinant glycoprotein expressedin spodoptera frugiperda (sf9) cellsshowed haemagglutinin (ha), neuraminidase (na) andhemadsorption activities. ha activity was detected inboth extra- and intra-cellular recombinant hn(rechnaf2240) samples. in addition, both ha and ... | 2000 | 19002985 |
| using cell size kinetics to determine optimal harvest time for spodoptera frugiperda and trichoplusia ni bti-tn-5b1-4 cells infected with a baculovirus expression vector system expressing enhanced green fluorescent protein. | infecting insect cells with a baculovirus expression vector system (bevs) is an increasingly popular method for the production of recombinant proteins. due to the lytic nature of the system, however, determining the optimal harvest time is critical for maximizing protein yield. we found that measuring the change in average diameter during the progress of infection with an automated cell analysis system (cedex hires, innovatis ag) could be used to determine the time of maximum protein production ... | 2007 | 19003016 |
| cell culture derived agmnpv bioinsecticide: biological constraints and bioprocess issues. | we have studied parameters for optimizing the spodoptera frugiperda (sf9) cell culture and viral infection for the production of anticarsia gemmatalis multiple nucleopolyhedrosis virus (agmnpv) polyhedra inclusion bodies (pibs) in shaker-schott or spinner bottles and bioreactors. we have assayed the k(l)a of the systems, initial cell seeding, cell culture volume, dissolved oxygen (do), multiplicity of infection (moi), nutrients consumption, and metabolites production. the medium surface oxygen t ... | 2005 | 19003030 |
| enhancement of sf9 cells and baculovirus production employing grace's medium supplemented with milk whey ultrafiltrate. | animal cells can be cultured both in basal media supplemented with fetal bovine serum (fbs) and in serum-free media. in this work, the supplementation of grace's medium with a set of nutrients to reduce fbs requirements in spodoptera frugiperda (sf9) cell culture was evaluated, aiming the production of anticarsia gemmatalis nucleopolyhedrovirus (agmnpv) at a cost lower than those for the production using sf900 ii medium. in grace's medium supplemented with glucose, pluronic f68 (pf68) and yeast ... | 2005 | 19003058 |
| biochemical analysis of hyphantria cunea npv attachment to spodoptera frugiperda 21 cells. | binding characteristics of hyphantria cunea nuclear polyhedrosis virus (hcnpv) to spodoptera frugiperda 21 (sf21) cells was determined. the cells displayed an affinity of 0.9 x 10(10) m(-1) with about 8900 binding sites per cell. the biochemical nature of hcnpv-binding sites on the cell surface was also partially elucidated. there were 45 to 49% reductions in hcnpv binding following the pretreatment of cells with three proteases, suggesting the involvement of a cellular protein component in viru ... | 1999 | 19003136 |
| insect cells respiratory activity in bioreactor. | specific respiration rate ( [formula: see text]) is a key parameter to understand cell metabolism and physiological state, providing useful information for process supervision and control. in this work, we cultivated different insect cells in a very controlled environment, being able to measure [formula: see text]. spodoptera frugiperda (sf9) cells have been used through virus infection as host for foreign protein expression and bioinsecticide production. transfected drosophila melanogaster (s2) ... | 2008 | 19003170 |
| characterisation of tetraploid and diploid clones of spodoptera frugiperda cell line. | we have isolated and characterised diploid and tetraploid clones from the normally heterologous spodoptera frugiperda (sf-9)cell line by dilution cloning technique. tetraploid clones were found to have cell sizes in excess of 35% larger than that of the diploid clones. in contrast, the maximum cell numbers achieved in batch cultures of diploid clones were on average 185% higher than the tetraploid cell numbers. growth rates and metabolic quotients during the exponential phase were similar for bo ... | 2004 | 19003226 |
| examining the relative activity of several dicistrovirus intergenic internal ribosome entry site elements in uninfected insect and mammalian cell lines. | comparisons of the relative activities of 11 intergenic region (igr) internal ribosome entry site (ires) elements of insect dicistrovirus with 5' ires elements of the hepatitis c and encephalomyocarditis viruses were performed in insect and mammalian cells. dual luciferase assays were performed to determine the most effective dicistrovirus igr ires in the lepidopteran cell lines sf9 (spodoptera frugiperda) and bmn (bombyx mori), and the dipteran cell lines s2 (drosophila melanogaster) and atc-10 ... | 2008 | 19008405 |
| investigating apoptosis: characterization and analysis of trichoplusia ni-caspase-1 through overexpression and rnai mediated silencing. | in both mammals and invertebrates, caspases play a critical role in apoptosis. although lepidopteron caspases have been widely studied in spodoptera frugiperda cells, this is not the case for trichoplusia ni cells, despite their widespread use for the production of recombinant protein and differences in baculovirus infectivity between the two species. we have cloned, expressed, purified and characterized tn-caspase-1 in several situations: in its overexpression, in silencing via rna interference ... | 2009 | 19027856 |
| [temperature effects on the mating frequency of anticarsia gemmatalis hüebner and spodoptera frugiperda (j.e. smith) (lepidoptera: noctuidae)]. | this paper deals with the influence of temperature on the mating frequency of two lepidopterans, anticarsia gemmatalis hübner and spodoptera frugiperda (j.e. smith), which use different strategies of allocation, and with the utilization of nutrients for their reproductive activities. the insects were reared on artificial diets at 25 masculinec, and the adults were exposed to five temperatures (15, 20, 25, 30, and 35 masculinec) to observe the influence of each temperature on their mating frequen ... | 2008 | 19061037 |
| toxicity of delta-phenothrin and resmethrin to non-target insects. | the susceptibility of adult house cricket, acheta domesticus (l.), adult convergent lady beetle, hippodamia convergens (guérin-méneville), and larval fall armyworm, spodoptera frugiperda (je smith), to resmethrin and delta-phenothrin synergized with piperonyl butoxide (pbo) was evaluated in a laboratory bioassay procedure. | 2009 | 19097021 |
| host strain specific sex pheromone variation in spodoptera frugiperda. | abstract: | 2008 | 19109878 |
| p64, a novel major virion dna-binding protein potentially involved in condensing the spodoptera frugiperda ascovirus 1a genome. | we recently identified 21 structural proteins in the virion of spodoptera frugiperda ascovirus 1a (sfav1a), a virus with a large, double-stranded dna genome of 157 kbp, which attacks species of the lepidopteran family noctuidae. the two most abundant virion proteins were the major capsid protein and a novel protein (p64) of 64 kda that contained two distinct domains not known previously to occur together. the amino-terminal half of p64 (residues 1 to 263) contained four repeats (a recently recog ... | 2009 | 19129454 |
| total synthesis of (-)-penifulvin a, an insecticide with a dioxafenestrane skeleton. | herein we report the first total synthesis of penifulvin a, a sesquiterpenoid with a novel dioxa-fenestrane structure. penifulvin a is a potent insecticide against the fall armyworm spodoptera frugiperda which causes enormous damage in the us by consuming foliage of a variety of field crops. a five-step racemic and an eight-step enantioselective route to the natural product and the determination of its absolute configuration are described. the key step involves a meta-photocycloaddition, giving ... | 2009 | 19140787 |
| proteomic analysis of the spodoptera frugiperda ascovirus 1a virion reveals 21 proteins. | the spodoptera frugiperda ascovirus 1a (sfav-1a) is a double-stranded dna virus that attacks lepidopteran larvae, in which it produces enveloped virions with complex symmetry which have an average diameter of 130 nm and length of 400 nm. here, we report identification of 21 sfav-1a-encoded proteins that occur in the virion, as determined by nano-liquid chromatography/tandem mass spectrometry. these included a helicase (orf009), nuclease (orf075), atpase (orf047), serine/threonine-like protein ki ... | 2009 | 19141444 |
| immunological properties of a fusion protein containing nucleocapsid protein and glycoprotein gn of hantaan virus. | escherichia coli and the baculovirus bac-to-bac system were used to express chimeric gene gns0.7 consisting of glycoprotein gn gene and the 0.7 kb fragment of s genome segment carrying nucleoprotein (n) gene of hantaan virus (htnv). the expressed fusion protein gnn0.7 was recognized by monoclonal antibodies (mabs) to htnv gn glycoprotein and n protein, respectively. its molecular mass as determined by western blot analysis corresponded to the predicted value. the mice immunized with insect spodo ... | 2008 | 19143481 |
| caterpillar footprints as host location kairomones for cotesia marginiventris: persistence and chemical nature. | herbivores walking over the epicuticular wax layer of a plant may leave tracks that disclose their presence to hunting predators or parasitoids. the braconid wasp cotesia marginiventris is a solitary parasitoid of young noctuid caterpillars. it can locate potential hosts from a distance by orienting toward the scent of herbivore-damaged plants. upon landing on the caterpillars' food plant, the female parasitoid searches for further cues (kairomones) that confirm the presence of a suitable host. ... | 2009 | 19153795 |
| use of by-products rich in carbon and nitrogen as a nutrient source to produce bacillus thuringiensis (berliner)-based biopesticide. | the amount and sources of carbon and nitrogen used to produce bacillus thuringiensis (berliner)-based biopesticide may influence the quality of the final product. the objective of this research was to test different levels of carbon and nitrogen: medium 1 - 1.5% maize glucose + 0.5% soy flour, medium 2 - 3.0% maize glucose + 1.0% soy flour, medium 3 - 1.0% maize glucose + 3.0% soy flour and medium 4 - luria bertani (lb) + salts (feso4, znso4, mnso4, mgso4). the seed culture was produced in lb me ... | 2008 | 19169559 |
| interaction of bacillus thuringiensis cry1 and vip3a proteins with spodoptera frugiperda midgut binding sites. | vip3aa, vip3af, cry1ab, and cry1fa were tested for their toxicities and binding interactions. vip3a proteins were more toxic than cry1 proteins. binding assays showed independent specific binding sites for cry1 and vip3a proteins. cry1ab and cry1fa competed for the same binding sites, whereas vip3aa competed for those of vip3af. | 2009 | 19181834 |
| ascoviruses: superb manipulators of apoptosis for viral replication and transmission. | ascoviruses are members of a recently described new family (ascoviridae) of large double-stranded dna viruses that attack immature stages of insects belonging to the order lepidoptera, in which they cause a chronic, fatal disease. ascoviruses have several unusual characteristics not found among other viruses, the most novel of which are their transmission by endoparasitic wasps and a unique cytopathology that resembles apoptosis. cell infection induces apoptosis and in some species is associated ... | 2009 | 19216438 |
| caspase inhibitor p35 is required for the production of robust baculovirus virions in trichoplusia ni tn-368 cells. | apoptosis can protect lepidopteran insects against baculovirus infection by limiting viral replication. baculoviruses counter this response by expressing anti-apoptotic proteins such as the caspase inhibitor p35, which is expressed by several baculoviruses including autographa californica mutiple nucleopolyhedrovirus (acmnpv). mutants of acmnpv that lack the p35 gene induce apoptosis in spodoptera frugiperda cells, and replication of these mutants is severely curtailed in s. frugiperda cell line ... | 2009 | 19218211 |
| octamer and heat shock elements regulate transcription from the acmnpv polyhedrin gene promoter. | the baculovirus expression vector system exploits the polyhedrin (polh) promoter for high expression of foreign proteins in insect cells. the mechanism of basal and hyperactivated transcription from this promoter, however, remains poorly understood. we have analyzed the 4-kb upstream region of the polh promoter; deletion of two separate parts of the 4-kb upstream region, harboring the oct binding site and the heat shock element, respectively, resulted in significant reduction of reporter gene ex ... | 2009 | 19221693 |
| do caterpillars secrete "oral secretions"? | the oral secretions or regurgitant of caterpillars contain potent elicitors of plant induced responses. these elicitors are recognized by host plants to differentiate between simple mechanical injury and the presence of herbivores. in some cases, this level of recognition is highly specific. despite the in-depth chemical characterization of these elicitors, little is known about the amounts delivered in regurgitant during feeding. in this study, we use a fluorescent dye to label regurgitant in o ... | 2009 | 19221841 |
| physiological, nutritional, and biochemical bases of corn resistance to foliage-feeding fall armyworm. | three corn (zea mays) germplasm lines [i.e., ab24e (susceptible control), mp708 (resistant control), and a locally selected partial inbred line faw7050 (resistant)] were examined for spodoptera frugiperda (j.e. smith; lepidoptera: noctuidae) resistance. nutritional [i.e., total protein content, amino acids, glucose, total nonstructural carbohydrates (tnc), protein to tnc (p/c) ratios] and biochemical (i.e., peroxidase and lipoxygenase 3) properties in the seedlings of these corn lines were exami ... | 2009 | 19221843 |
| ecological modulation of plant defense via phytochrome control of jasmonate sensitivity. | for plants, the tradeoff between resource investment in defense and increased growth to out-compete neighbors creates an allocation dilemma. how plants resolve this dilemma, at the mechanistic level, is unclear. we found that arabidopsis plants produced an attenuated defense phenotype under conditions of crowding and when exposed to far-red (fr) radiation, a light signal that plants use to detect the proximity of neighbors via the photoreceptor phytochrome. this phenotype was detectable through ... | 2009 | 19251652 |
| cloning and expression of simian rotavirus spike protein (vp4) in insect cells by baculovirus expression system. | vp4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. vp4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. | 2009 | 19252673 |
| can the amount of corn acreage predict fall armyworm (lepidoptera: noctuidae) infestation levels in nearby cotton? | fall armyworm, spodoptera frugiperda (j.e. smith) (lepidoptera: noctuidae), is a major pest of corn, zea mays l., and a significant, but more sporadic, pest of cotton, gossypium hirsutum l., in the western hemisphere. previous studies showed that the cotton infestations primarily involve a fall armyworm subpopulation known as the "corn-strain" for which corn is the preferred host plant. it was suggested that the fall armyworm infesting cotton originated in corn and spread into secondary hosts as ... | 2009 | 19253639 |
| taxonomic and biological characterization of steinernema rarum found in the southeastern united states. | two steinernema isolates found in louisiana and mississippi were later identified as isolates of s. rarum. dna sequences of its regions of the united states isolates are identical with sequences of argentinean s. rarum strains samiento and noetinger and differ by two bases from the arroyo cabral isolate from córdoba, argentina. sem observations revealed several new structures in the isolates from the us: female face views have a hexagonal-star perioral disc and eye-shaped lips; some females do n ... | 2006 | 19259427 |
| mixtures of complete and pif1- and pif2-deficient genotypes are required for increased potency of an insect nucleopolyhedrovirus. | the insecticidal potency of a nucleopolyhedrovirus population (sfnic) that infects spodoptera frugiperda (lepidoptera) is greater than the potency of any of the component genotypes alone. occlusion bodies (obs) produced in mixed infections comprising the complete genotype and a deletion genotype are as pathogenic as the natural population of genotypes from the field. to test whether this increased potency was due to the deletion or to some other characteristic of the deletion variant genome, we ... | 2009 | 19264787 |
| multiple factors conferring high radioresistance in insect sf9 cells. | sf9, a lepidopteran cell line isolated from the fall armyworm, spodoptera frugiperda, was shown to be significantly more resistant to growth inhibition and apoptosis induction effects of x-ray irradiation than several human cell lines of different origins. the single-cell electrophoresis technique revealed that sf9 cells showed lower x-ray irradiation-induced dna damage as well as better efficiency at repairing these damages. in addition, sf9 cells were lower in both background and x-ray irradia ... | 2009 | 19264841 |
| molecular characterization of a tia-1-like rna-binding protein in cells derived from the fall armyworm spodoptera frugiperda (lepidoptera: noctuidae). | a complementary dna encoding a tia-1-type rna-binding protein (sftrn-1) was isolated from cultured cells of the fall armyworm, spodoptera frugiperda (lepidoptera: noctuidae), to characterize its function. the deduced 388-amino acid sequence of sftrn-1, which possessed three rna recognition motifs (rrms) followed by a c-terminal auxiliary domain, showed significant homology with mammalian tia-1/tiar and silkworm bmtrn-1, factors important in the metabolism of transcripts. it was found that inhibi ... | 2009 | 19270389 |
| ectoparasitic acugutturid nematodes of adult lepidoptera. | noctuidonema guyaneme is an interesting ectoparasite of adult lepidoptera that feeds on hosts from at least five families with its long stylet. noctuidonema guyanense spends its entire life on the adult moth and is sustained as it is passed from moth to moth during host mating. overlapping host generations are essential for parasite survival. this nematode occurs throughout tropical and subtropical america and is transported by at least one of its hosts, spodoptera frugiperda, during migration t ... | 1996 | 19277339 |
| quantification of invasion of two strains of steinernema carpocapsae (weiser) into three lepidopteran larvae. | studies with last instar larvae of the fall armyworm, spodoptera frugiperda (j. e. smith), the black cutworm, agrotis ipsilon (hufnagel), and the greater wax moth, galleria mellonella (l.) were used to quantify the invasive ability of two strains (all and mexican) of steinernema carpocapsae and to determine how factors in the bioassay procedure affect both nematode invasion and host mortality. nematode invasive ability was variable, with 10-50% of nematodes successfully infecting the host. the p ... | 1993 | 19279755 |
| evaluation of the spatial pattern of steinernema riobravis in corn plots. | the vertical and horizontal spatial patterns of a naturally occurring population of the entomopathogenic nematode steinernema riobravis (rhabditida: steinernematidae) were investigated in corn field soil by laboratory and field bioassays. this nematode appears to be endemic to the lower rio grande valley of texas, where it was found parasitizing prepupae and pupae of both corn earworm, helicoverpa zea, and fall armyworm, spodoptera frugiperda (lepidoptera: noctuidae). corn earworm prepupa was th ... | 1994 | 19279865 |
| effect of host age and nematode strain on susceptibility of spodoptera frugiperda to steinernema feltiae. | median lethal concentrations (lc) were determined for four nematode populations (two strains of steinernema feltiae, a s. feltiae hybrid, and s. bibionis) against fifth-instar fall armyworm (spodoptera frugiperda) larvae and for the most virulent of these nematodes against different instars and stages of the insect. based on lack of overlap of 95% fiducial limits, there were significant differences in virulence among the four nematodes. the lc ranged from 7.6 to 33.3 nematodes/ 0.7 ml water, and ... | 1988 | 19290189 |
| stress response of a p53 homologue in the radioresistant sf9 insect cells. | to investigate homology and stress response of p53 (a 53 kda tumor suppressor protein) orthologue in sf9 lepidopteran insect cell line that exhibits very high radioresistance. | 2009 | 19296337 |
| the purification and application of biologically active recombinant steroid cytochrome p450 21-hydroxylase: the major autoantigen in autoimmune addison's disease. | in primary adrenocortical failure (addison's disease) caused by autoimmunity, autoantibodies to the steroidogenic cytochrome p450 enzyme 21-hydroxylase (21oh) are detected in the majority of patients. it is currently uncertain whether the autoantibodies themselves participate in the pathogenesis, or if they merely reflect an on-going t cell mediated response. the identification of t cells reactive with 21oh, if any, has been hampered by the lack of a high-quality antigen. in the current study re ... | 2009 | 19329278 |
| g-protein coupled receptor array technologies: site directed immobilisation of liposomes containing the h1-histamine or m2-muscarinic receptors. | this paper describes a novel strategy to create a microarray of g-protein coupled receptors (gpcrs), an important group of membrane proteins both physiologically and pharmacologically. the h(1)-histamine receptor and the m(2)-muscarinic receptor were both used as model gpcrs in this study. the receptor proteins were embedded in liposomes created from the cellular membrane extracts of spodoptera frugiperda (sf9) insect cell culture line with its accompanying baculovirus protein insert used for ov ... | 2009 | 19337994 |
| antifeedant activity of ethanolic extract from flourensia oolepis and isolation of pinocembrin as its active principle compound. | the ethanolic extract from flourensia oolepis aerial parts showed strong antifeedant activity against the pest larvae, epilachna paenulata, with an antifeedant index (ai%) of 99.1% at 100 microg/cm(2). based on chromatographic fractionation of the extract, guided by bioassays on e. paenulata, the flavanone pinocembrin (1) was isolated as the most active principle. in a choice assay, compound 1 showed strong antifeedant activity against e. paenulata, xanthogaleruca luteola and spodoptera frugiper ... | 2009 | 19342224 |
| [morphology of the alimentary canal of spodoptera frugiperda (j e smith) larvae (lepidoptera: noctuidae) fed on neem-treated leaves]. | research involving plants with insecticide activity evolved significantly in the last decades. among these plants, the neem tree, azadirachta indica, is commonly used against several insects, mainly lepidoptera. the neem efficiency depends on the target insect and on the concentration used. a barrier against potential toxic agents ingested together with the food is the alimentary canal. thus, this research aimed to describe the histology of the alimentary canal of spodoptera frugiperda (j e smit ... | 2009 | 19347100 |
| [survival and larval development of spodoptera frugiperda (j e smith) (lepidoptera: noctuidae) on alternatives host]. | two bioassays were conducted to evaluate the suitability of host plants of fall armyworm (faw), spodoptera frugiperda (j e smith), in the brazilian agro-ecosystem. larval development and survival were analyzed by infesting leaves of maize, grain sorghum, johnson grass, soybean, brachiaria and tobacco with faw newly hatched larvae in a no choice test. no significant differences of survival were observed among insects reared on different hosts, except for tobacco, where no survivors were recorded. ... | 2009 | 19347104 |
| cell density effect in the baculovirus-insect cells system: a quantitative analysis of energetic metabolism. | the cell density effect (i.e., the drop in the specific productivity in the baculovirus-insect cells expression system when cells are infected at high cell densities) has been extensively described in the literature. in this article, a model for the central metabolism of serum-free suspension cultures of spodoptera frugiperda sf9 cells is proposed and used to investigate the metabolic basis for this phenomenon. the main metabolic pathways (glycolysis, pentose phosphate pathway, tricarboxylic aci ... | 2009 | 19459142 |
| time-shifted reproductive behaviours among fall armyworm (noctuidae: spodoptera frugiperda) host strains: evidence for differing modes of inheritance. | the noctuid moth spodoptera frugiperda consists of two strains associated with different larval host plants (most notably corn and rice). these strains exhibit differential temporal patterns of female calling and copulation during scotophase, with the corn strain more active earlier in the night. we investigated strain-specific constraints in reproductive timing, mating interactions between the two strains, and the mode of inheritance of timing of female calling, male calling, copulation and ovi ... | 2009 | 19467132 |
| distribution of elongation factor-1alpha in larval tissues of the fall armyworm, spodoptera frugiperda. | elongation factor-1alpha (ef-1alpha) promotes the delivery of aminoacyl-trna to the acceptor site of the ribosome during protein synthesis. the enzyme has a number of additional functions, including regulation of apoptosis and interaction with the cytoskeleton. we determined the distribution of ef-1alpha in larval tissues of the fall armyworm, spodoptera frugiperda , with a monoclonal antibody generated to ef-1alpha from sf21 cells, a cell line developed from ovarian tissue of s. frugiperda. enz ... | 2006 | 19537984 |
| high yield expression of biologically active recombinant full length human tuftelin protein in baculovirus-infected insect cells. | tuftelin is an acidic protein expressed at very early stages of mouse odontogenesis. it was suggested to play a role during epithelial-mesenchymal interactions, and later, when enamel formation commences, to be involved in enamel mineralization. tuftelin was also detected in several normal soft tissues of different origins and some of their corresponding cancerous tissues. tuftelin is expressed in low quantities, and undergoes degradation in the enamel extracellular matrix. to investigate the st ... | 2009 | 19539764 |
| characterization of short-lived intermediates produced during replication of baculovirus dna. | in this report the short-lived dna replication intermediates produced in both uninfected and autographa californica multiple nucleopolyhedrovirus (acmnpv) infected spodoptera frugiperda cells were characterized. the methods used included pulse-labeling of dna in permiabilized cells, treatment of nascent dna with mung bean nuclease, and electrophoresis in neutral and alkaline agarose gels. in contrast to uninfected cells that produced a population of small dna fragments of about 200bp, a populati ... | 2009 | 19560496 |