| pf4, a fmrfamide-related peptide, gates low-conductance cl(-) channels in ascaris suum. | here we describe the actions of the peptide lys-pro-asn-phe-ile-arg-phe-nh(2), or pf4, on inside-out membrane patches (n=164), recorded from vesicles derived from ascaris suum somatic muscle cells. we observed numerous, small-amplitude cl(-) channels in the membrane patches. the conductance of the cl(-) channels ranged from 1.09 to 7.07 ps, the open probability (p(open)) ranged from 0.047+/-0.015 (mean+/-s.e.m.) at 0 microm pf4 to 0.156+/-0.026 at 0.1 microm pf4. the channel mean open time was m ... | 2002 | 12450564 |
| cloning and characterisation of a highly immunoreactive 37 kda antigen with multi-immunoglobulin domains from the swine roundworm ascaris suum. | antigens from larval stages of ascaris suum have been shown to induce protection against challenge infection with infective a. suum eggs. we previously identified several antigens that reacted strongly with serum from pigs inoculated with infective eggs containing l3. in this study, we isolated an antigen with a molecular mass of 37 kda and a pi of 4.8 (as37) from a. suum infective eggs using two-dimensional electrophoresis, and obtained a full-length cdna by reverse transcription-polymerase cha ... | 2002 | 12464420 |
| structure-activity relationships of an inhibitory nematode fmrfamide-related peptide, sdpnflrfamide (pf1), on ascaris suum muscle. | fmrfamide-related peptides are widespread among the nematoda. among them is a family of extended pnflrfamide peptides encoded on the flp-1 peptide precursor gene in caenorhabditis elegans. the most studied peptide from this series is sdpnflrfamide (pf1). each residue in this peptide was sequentially substituted with either alanine or the corresponding d-isomer of the native amino acid in order to define structure-function relationships in this peptide using an ascaris suum muscle tension assay. ... | 2002 | 12464423 |
| role of dihydrolipoyl dehydrogenase (e3) and a novel e3-binding protein in the nadh sensitivity of the pyruvate dehydrogenase complex from anaerobic mitochondria of the parasitic nematode, ascaris suum. | the pyruvate dehydrogenase complex (pdc) plays changing roles during the aerobic-anaerobic transition in the life cycle of the parasitic nematode, ascaris suum. however, the dihydrolipoyl dehydrogenase (e3) subunit appears to be identical in all stages, despite the fact that the pdc is less sensitive to nadh inhibition in anaerobic muscle. therefore, we have cloned cdnas encoding e3 and a novel anaerobic-specific e3-binding protein (e3bp) that lacks the terminal lipoyl domain found in e3bps from ... | 2002 | 12467981 |
| in vitro studies on the effects of flubendazole against toxocara canis and ascaris suum. | adult toxocara canis and ascaris suum were incubated in vitro in media containing 0.1, 1, 10 or 100 micro g/ml flubendazole in order to study drug-derived effects. this incubation was done for 8 h and repeated (in some groups) after 24 h for another 8 h. the onset and intensity of flubendazole-derived effects were dosage-dependent and time-dependent, i.e. the same grade of damage was reached when incubating for a longer period at a low dosage or for a shorter period in medium containing a high a ... | 2003 | 12474046 |
| ascarid nematodes in domestic and wild terrestrial mammals. | the biology of the ascarid nematodes has been discussed in the context of their important economic role in farm animals, pet animals and zoo animals with special attention to carnivores and primates. in farm animals, infection with the most common roundworm of horses (parascaris equorum) and swine (ascaris suum) depend on many factors such as environmental conditions (larval development in the egg and egg survival), age of the host, breed, husbandry system, hygiene and treatment schedule. the mo ... | 2002 | 12512563 |
| immunohistochemical distribution of antigens in liver of infected and immunized pigs with ascaris suum. | in the present work, we carry out an immunopathological study of the swine ascariosis, under different conditions (control, infection and immunization). twenty-one iberian pigs were used and divided in seven groups. groups 1 and 2 were the uninfected and challenged controls, respectively. groups 3 and 4 were weakly infected with increasing doses of ascaris suum eggs and treated with pyrantel (group 4). groups 5-7 were immunized with 14, 42 and 97 kda proteins from the parasite, respectively. gro ... | 2003 | 12523975 |
| af2 interaction with ascaris suum body wall muscle membranes involves g-protein activation. | kheylrf-nh(2) (af2) is the most abundant fmrfamide-related peptide (farp) in ascaris suum and also in many other parasitic and free-living nematodes. the af2 abundance in the highly diverse nematodes and its potent and profound effects on the neuromuscular systems make af2 and its receptor(s) very attractive targets for the discovery of novel broad-spectrum anthelmintics. although farp receptors are believed to belong to the large family of g-protein coupled receptors (gpcrs), to date no af2 rec ... | 2003 | 12565883 |
| [the protective immunity in mice immunized with fhgst or asgst against schistosoma japonicum cercariae]. | to study the protective immunity against schistosoma japonicum (sj) cercariae challenge in mice immunized with fhgst or asgst. | 2000 | 12567471 |
| [observation on the survival and invasibility of ascaris suum egg preserved at low temperature for 5 years]. | | 2002 | 12567985 |
| eosinophilic inflammation and airway hyper-responsiveness are profoundly inhibited by a helminth (ascaris suum) extract in a murine model of asthma. | the increase of atopic disorders in developed countries has been associated with the decline of infectious diseases, including helminthic infections. we have already demonstrated that adult worm extracts from ascaris suum (asc) suppress the ige antibody production against unrelated antigens. | 2002 | 12569989 |
| attempts to separate female ascaris suum antigen and to investigate its partial characterization. | the location and separation of ascaris suum antigen for serological testing was investigated. the antigenic constituent was rich in the ovary of the adult worm and was obtained by dialysis with 50% ammonium sulphate saturated solution. sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblotting analysis demonstrated that the heat labile antigenic preparation showed one major and seven faint bands. the major band seemed also to be a glycoprotein. the sera from pigs with/without ... | 2003 | 12573606 |
| the effect of the anthelmintic emodepside at the neuromuscular junction of the parasitic nematode ascaris suum. | here we report on the action of the novel cyclo-depsipeptide anthelmintic, emodepside, on the body wall muscle of the parasitic nematode, ascaris suum. emodepside caused (i) muscle relaxation, (ii) inhibition of muscle contraction elicited by either acetylcholine (ach), or the neuropeptide, af2 (kheylrfamide) and (iii) a rapid relaxation of muscle tonically contracted by ach. the inhibitory action of emodepside on the response to ach was not observed in a denervated muscle strip, indicating that ... | 2003 | 12613766 |
| induction of asabf (ascaris suum antibacterial factor)-type antimicrobial peptides by bacterial injection: novel members of asabf in the nematode ascaris suum. | recently, invertebrate models have been widely used for the study of innate immunity. nematodes are novel potential candidates because of the experimental advantages of caenorhabditis elegans. however, whether nematodes have active immune responses is still ambiguous. previously, we reported asabf (ascaris suum antibacterial factor)-type antimicrobial peptides in the parasitic nematode ascaris suum and the genetic model nematode c. elegans. further screening of a cdna library and an expressed-se ... | 2003 | 12617723 |
| pathogenic intestinal parasites and bacterial agents in solid wastes. | to determine the profile of potentially pathogenic enteric parasites and bacterial agents in municipal refuse dumps in ibadan, nigeria. | 2002 | 12630495 |
| effects of kheylrfamide and knefirfamide on cyclic adenosine monophosphate levels in ascaris suum somatic muscle. | kheylrf-nh(2) (af2) is a fmrfamide-related peptide (farp) present in parasitic and free-living nematodes. at concentrations as low as 10 pm, af2 induces a biphasic tension response, consisting of a transient relaxation followed by profound excitation, in neuromuscular strips prepared from ascaris suum. in the present study, the effects of af2 on cyclic adenosine monophosphate (camp), cyclic guanosine monophosphate (cgmp) and inositol-1,4,5-triphosphate (ip(3)) levels were measured following musc ... | 2003 | 12633657 |
| immunomodulatory properties of ascaris suum glycosphingolipids - phosphorylcholine and non-phosphorylcholine-dependent effects. | immunomodulatory properties of phosphorylcholine (pc)-containing glycosphingolipids from ascaris suum were investigated utilizing immune cells from balb/c mice. proliferation of splenic b cells induced either via f(ab')2 fragments of anti-murine ig (anti-ig) or lps was significantly reduced when the glycosphingolipids were present in the culture medium. however whereas the lps-mediated effect was dependent on the pc moiety of the glycosphingolipids, the result generated when using anti-ig was no ... | 2002 | 12654088 |
| critical role of il-5 in antigen-induced pulmonary eosinophilia, but not in lymphocyte activation. | infiltration of antigen-specific cd4+ t cells and production of interleukin (il)-5 in inflammatory regions play a central role in antigen-induced pulmonary eosinophilia. genetically il-5-deficient mice lack antigen-induced airway eosinophilia, but little is known about the role of il-5 in accumulation and activation of cd4+ t cells in the lung and in airway hyperresponsiveness (ahr). ascaris suum extract (asc) has been used to induce airway eosinophilia and analyze airway inflammation in il-5 re ... | 2003 | 12660425 |
| augmentation by bursaphelenchus xylophilus, a pine wood nematode, of polyclonal ige production induced by lipopolysaccharide plus interleukin-4 in murine splenocytes. | bursaphelenchus xylophilus (b. xylophilus) is a pine wood nematode that is known to cause pine wilt disease. we report here that b. xylophilus extracts augmented the polyclonal immunoglobulin e (ige) production induced by lipopolysaccharide (lps) plus interleukin-4 (il-4) both in murine splenocytes and purified b cells as determined by elisa and elispot assays, but they did not cause such a promotion in the absence of either lps or il-4. we also observed that the antigen-nonspecific ige levels w ... | 2003 | 12686761 |
| evolution of ascariasis in humans and pigs: a multi-disciplinary approach. | the nematode parasite ascaris lumbricoides infects the digestive tracts of over 1.4 billion people worldwide, and its sister species, ascaris suum, has infected a countless number of domesticated and feral pigs. it is generally thought that the putative ancestor to these worms infected either humans or pigs, but with the advent of domestication, they had ample opportunity to jump to a new host and subsequently specialize and evolve into a new species. while nuclear dna makers decisively separate ... | 2003 | 12687761 |
| precision and accuracy of an assay for detecting ascaris eggs in various biosolid matrices. | this paper presents quality assurance data and quality control data on the recovery of ascaris suum eggs from various biosolid matrices: acid-treated, alkaline-treated, amended-soil blended, and lagoon stored biosolids. over a period of years, the same procedure, the "tulane method," was performed on different matrices, and in this work, the data collected on the recovery of the eggs from the different matrices is examined and compared. the egg recoveries are discussed in terms of precision (the ... | 2003 | 12691891 |
| the polyprotein and far lipid binding proteins of nematodes: shape and monomer/dimer states in ligand-free and bound forms. | nematodes produce two classes of small, helix-rich fatty acid- and retinol-binding proteins whose structures and in vivo functions remain to be elucidated. these are the polyprotein allergens (npa) and the far proteins. the solution properties of recombinant forms of these proteins from parasitic [ascaris suum (as) and onchocerca volvulus (ov)] and free-living [caenorhabditis elegans (ce)] nematodes have been examined. analytical ultracentrifugation (auc) showed that, contrary to previous findin ... | 2003 | 12692694 |
| common structural properties specifically found in the csalphabeta-type antimicrobial peptides in nematodes and mollusks: evidence for the same evolutionary origin? | the structural properties of the ascaris suum antibacterial factor (asabf)-type antimicrobial peptides, isolated from nematodes, were compared with the csalphabeta-type antimicrobial peptides found in other organisms. the spacing of the half-cystine residues, cysteine pairings, and organization of the precursor were different from the 'classical' csalphabeta-type antimicrobial peptides, such as drosomycin and plant defensins, and identical only to the mgd and myticin in mollusks. in addition, ab ... | 2003 | 12697306 |
| the potential for disinfection of separated faecal matter by urea and by peracetic acid for hygienic nutrient recycling. | no efficient, reliable, and scale independent disinfection methods for toilet waste are available today for safe recycling of plant nutrients. therefore, two chemical treatment methods, addition of urea or of paa (a quaternary mixture of 15% peracetic acid, 15% hydrogen peroxide and 30% acetic acid), were evaluated for disinfection of faecal matter.degradation of the added urea resulted in 30 g of ammonia nitrogen per kilogram of treated matter and a ph increase to approximately 9.3. this produc ... | 2003 | 12699934 |
| heterologous expression of ascaris suum cytochrome b5 precursor protein: a histidine-tagged full-length presequence is correctly processed to transport the mature protein to the periplasm of escherichia coli. | the cytochrome b(5) of the body wall of adult ascaris suum, a porcine parasitic nematode, is a novel type of cytochrome b(5). it is a soluble protein that lacks the cooh-terminal membrane-anchoring domain found in erythrocyte cytochrome b(5), but possesses an nh(2)-terminal extension (presequence) of 30 amino acids that are missing from the 82-residue protein purified from the nematode tissues [yu, y., yamasaki, h., kita, k., and takamiya, s., 1996, arch. biochem. biophys. 328, 165-172]. the nem ... | 2003 | 12729624 |
| ascaris nematodes from pig and human make three antibacterial peptides: isolation of cecropin p1 and two asabf peptides. | organisms co-habiting with bacteria have developed efficient bactericidal agents to control their microbe-rich environment. the ascaris nematode lives in its final development stages in the gut of its host and is believed to feed on bacteria. ascaris suum survive in pig intestine while a. lumbricoides is the principal species in humans. here we show that a. suum and a. lumbricoides both produce linear (cecropin p1) and cysteine-rich (asabf) peptides with activity against either gram-negative or ... | 2003 | 12737319 |
| isolation and characterization of the stage-specific cytochrome b small subunit (cybs) of ascaris suum complex ii from the aerobic respiratory chain of larval mitochondria. | we recently reported that ascaris suum mitochondria express stage-specific isoforms of complex ii: the flavoprotein subunit and the small subunit of cytochrome b (cybs) of the larval complex ii differ from those of adult enzyme, while two complex iis share a common iron-sulfur cluster subunit (ip). in the present study, a. suum larval complex ii was highly purified to characterize the larval cytochrome b subunits in more detail. peptide mass fingerprinting and n-terminal amino acid sequencing sh ... | 2003 | 12742584 |
| effect of aerobic and anaerobic digestion on the viability of cryptosporidium parvum oocysts and ascaris suum eggs. | the viability of cryptosporidium parvum oocysts and ascaris suum eggs inoculated into aerobic and anaerobic digesters was measured. the digesters were maintained at 37 degrees c, 47 degrees c, and 55 degrees c, with 10-day detention times. eggs and oocysts were added to each digester in a single spike or in chambers placed in the digesters for varying periods. oocysts were inactivated very rapidly in all systems as determined by a dye permeability assay, > 99% inactivated after 10 days at 37 deg ... | 2003 | 12745337 |
| a role for cd44 in an antigen-induced murine model of pulmonary eosinophilia. | previous studies established that il-5-producing cd4(+) t cells play a pivotal role in allergic respiratory inflammation. it was also reported that cd4(+) t cells express higher levels of cd44 in the airway than in peripheral blood of patients with allergic respiratory diseases. we have used experimental pulmonary eosinophilia induced in mice by ascaris suum (asc) extract to investigate the role of cd44 in the development of allergic respiratory inflammation. intraperitoneal administration of an ... | 2003 | 12750406 |
| serological responses to ascaris suum adult worm antigens in iberian finisher pigs. | adult ascaris suum were dissected to obtain different worm components (body wall, body fluid, ovaries, uterus and oesophagus) which were used as antigens when testing 95 sera of naturally a. suum-infected iberian pigs by enzyme-linked immunosorbent assay (elisa) and western blot (wb). pigs with patent ascaris infections had significantly lower elisa optical density values than pigs without adult worms when using the body fluid and the body wall as antigens. a poor negative correlation was found ... | 2003 | 12756071 |
| cysteinyl leukotrienes in porcine acute allergic airway reactions: bronchial and vascular effects. | the role of cysteinyl leukotrienes (cyslts) in the acute allergic airway reaction in the pig was investigated, with focus on the effects on the bronchial circulation, and compared with histamine-induced effects. | 2003 | 12813622 |
| biophysical characterization of a large conductance anion channel in hypodermal membranes of the gastrointestinal nematode, ascaris suum. | patch-clamp recordings from muscle- and cuticle-facing hypodermal membranes of the gastrointestinal nematode ascaris suum reveal a high-conductance, voltage- sensitive ca(2+) -dependent cl(-) channel. the hypodermal channel has a conductance of 195 ps in symmetrical 160 mm nacl. the open probability of the channel is highly voltage-sensitive, and channel activity is not observed when ca(2+) is reduced to <100 microm. the channel is permeable to organic anions that are major end-products of carbo ... | 2003 | 12814789 |
| investigating the impact of helminth products on immune responsiveness using a tcr transgenic adoptive transfer system. | helminth infections and their products have a potent immunomodulatory effect on the host immune system and can impair immune responses against unrelated ags. in vitro studies have suggested that the immunomodulation by helminth extracts may be the result of bystander response bias toward a th2 phenotype and/or an ag-specific t lymphocyte proliferative hyporesponsiveness. the aim of this study was to determine the role of these potential mechanisms of immunosuppression in vivo. therefore, using a ... | 2003 | 12817029 |
| inorganic pyrophosphatase in the roundworm ascaris and its role in the development and molting process of the larval stage parasites. | inorganic pyrophosphatase (ppase) is an important enzyme that catalyzes the hydrolysis of inorganic pyrophosphate (ppi) into ortho-phosphate (pi). we report here the molecular cloning and characterization of a gene encoding the soluble ppase of the roundworm ascaris suum. the predicted a. suum ppase consists of 360 amino acids with a molecular mass of 40.6 kda and a pi of 7.1. amino acid sequence alignment and phylogenetic analysis indicates that the gene encodes a functional family i soluble pp ... | 2003 | 12823552 |
| requirement of modified residue m1a9 for ef-tu binding to nematode mitochondrial trna lacking the t arm. | most of nematode mitochondrial (mt) trnas lacking the t arm have 1-methyladenosine (m1a) at position 9. to investigate the effect of m1a, we constructed a nematode ascaris suum mt trna(met) containing only m1a9 as the modified nucleoside by means of molecular surgery. although the unmodified a. suum mt met-trna(met) did not bind to nematode mt ef-tu, the m1a9-containing trna bound to the ef-tu, suggesting that m1a at position 9 is necessary for binding of nematode mt trnas lacking the t arm to t ... | 2001 | 12836352 |
| crystallographic studies on ascaris suum nad-malic enzyme bound to reduced cofactor and identification of an effector site. | the crystal structure of the mitochondrial nad-malic enzyme from ascaris suum, in a quaternary complex with nadh, tartronate, and magnesium has been determined to 2.0-a resolution. the structure closely resembles the previously determined structure of the same enzyme in binary complex with nad. however, a significant difference is observed within the coenzyme-binding pocket of the active site with the nicotinamide ring of nadh molecule rotating by 198 degrees over the c-1-n-1 bond into the activ ... | 2003 | 12853453 |
| the ovijector of ascaris suum: multiple response types revealed by caenorhabditis elegans fmrfamide-related peptides. | caenorhabditis elegans possesses 22 fmrfamide-like peptide (flp) genes predicted to encode 60 different fmrfamide-related peptides with a range of c-terminal signatures. peptides from five flp genes (1, 6, 8, 9 and 14) are known to modulate the ovijector of ascaris suum in vitro. this study examines the physiological effects of peptides from the remaining 17 flp genes such that the variety of fmrfamide-related peptide-induced ovijector response types can be delineated. five categories of respons ... | 2003 | 12865086 |
| [he staining of uterus cells of ascaris suum by egg albumin-glycerine smear technique]. | | 2003 | 12884631 |
| structure and organization of the mitochondrial genome of the canine heartworm, dirofilaria immitis. | this study determined the complete mitochondrial (mt) genome sequence of the canine heartworm, dirofilaria immitis, and compared its structure, organization and other characteristics with onchocerca volvulus and other secernentean nematodes. the d. immitis mt genome is 13814 bp in size and contains 36 of the 37 genes typical of metazoan organisms, and lacks the atp synthetase subunit 8 gene. all of the genes are transcribed in the same direction. for the entire genome, the nucleotide contents ar ... | 2003 | 12885187 |
| some new aspects of the pathology, pathogenesis, and aetiology of disseminated lung lesions in slaughter pigs. | from 40 pigs rejected for human consumption at slaughter due to an apparent presence of pyemic lung lesions (defined as disseminated processes containing pus and/or necrotic material), the lungs, spleen, liver, and kidneys were subjected to an extended macroscopic examination. several lung lesions were sampled from each animal for histological and bacteriological examination. samples from the kidneys and spleens were also subjected to bacteriological examination. at gross level, four groups of l ... | 2003 | 12887504 |
| [studies on the comparative migration patterns of ascaris suum larvae between primary and re-infected mice] | in the present study, the effect of primary infection to reinfection with ascaris suum larvae was experimented in mouse model. mice were challenged with l,000 infective stage eggs of ascaris suum. the embryonated eggs were directly introduced into stomach of mice. reinfection was performed at 50 days after the primary infection with same method as primary infection. mice were sacrificed 3, 5, 7, 10, 15 and 20 days after infection in both groups respectively. larvae collected from livers and lung ... | 1985 | 12888668 |
| ige production is involved in butyrate-enhanced nk cell activity in vivo. | it has been demonstrated that patients with asthma have a large number of nk cells and show a stronger nk activity. these results indicate that nk cell activity may be related to total ige level in serum in healthy subjects. previously, we have found that sodium butyrate (nabu) markedly enhanced the il-4-induced ige production in the lps-stimulated murine splenocytes in vitro, and inductive rat ige production in vivo, and enhanced the nk cell activity ex vivo. we hypothesized that the ige produc ... | 2001 | 12901494 |
| [a comparative analysis of various parts of ascaris suum with respect to their protein composition] | for the purpose of making a comparative study of protein compositions in ascaris suum by sexes and body parts, extracts were prepared from whole bodies, body walls, genital organs, digestive organs and body fluid, of both sexes. and electrophoretic analysis was conducted using polyacrylamide slab gel in the presence of sodium dodecyl sulfate. the results were as follows: 1. in this study, protein bands of each part were separated in the largest number and most clearly under 8%~12 % (10 %) gradie ... | 1981 | 12902713 |
| [an epidemiological study on zoonoses in korea] | helminthic zoonoses are of significant importance to the public health and to the socioeconomic consequences especially in lost-meat, animal products and animal labor. for past several decades in korea, it has been recognized that endoparasitic infections among domestic animals are extremely common and many of the helminth parasites of man have been acquired from, or are shared with domestic animals. however, there was no survey of a nationwide scale on the prevalence of animal helminthiasis; an ... | 1981 | 12902720 |
| [immunoelectrophoretic studies of several helminths] | in an attempt to investigate the antigen-antibody relations and the value of immunodiagnosis for several helminths, ouchterlony tests and immunoelectrophoreses were carried out. taenia saginata, cysticercus sp. of cestodes, clonorchis sinensis, fasciola hepatica and paramphistomum sp. of trematodes,and ascaris suum of nematodes were used as antigens. on the other hand, antisera were obtained by injecting 0.5 ml each of the above antigens and the same amount of complete freund's adjuvant into rab ... | 1979 | 12902755 |
| ascaris suum nad-malic enzyme is activated by l-malate and fumarate binding to separate allosteric sites. | the kinetic mechanism of activation of the mitochondrial nad-malic enzyme from the parasitic roundworm ascaris suum has been studied using a steady-state kinetic approach. the following conclusions are suggested. first, malate and fumarate increase the activity of the enzyme in both reaction directions as a result of binding to separate allosteric sites, i.e., sites that exist in addition to the active site. the binding of malate and fumarate is synergistic with the k(act) decreasing by >or=10-f ... | 2003 | 12911313 |
| [a study on the lipids of ascaris suum ova] | the present study was undertaken to observe the quality and quantity of lipid constituents by the developing ascaris suum eggs. the collected eggs from the uterus of a. suum were classified into 3 groups, i.e., single cell stage, morula stage and embryonated eggs, and were subjected to analyse their lipid fractions. to obtain the morula stage eggs, 10 to 11 incubation days at 30 degree c were needed and for the embryonated eggs, 30 to 31 days were lasted. at the time of experiment, their indices ... | 1977 | 12913434 |
| diagnosis of parasitic zoonoses by immunoenzymatic assays--analysis of cross-reactivity among the excretory/secretory antigens of fasciola hepatica, toxocara canis, and ascaris suum. | several parasitic infections such fasciolosis, toxocariosis or ascariosis are important zoonoses. during the infection with fasciola hepatica, toxocara canis and ascaris suum, an important intraorganic phase in their hosts takes place, releasing antigens responsible for a humoral immune response, which enables the diagnosis of that parasitosis. a study to identify the existence of cross-reactivity among the excretory/ secretory antigens of f. hepatica, t. canis and a. suum was developed. one gro ... | 2003 | 12916704 |
| mice intranasally immunized with a recombinant 16-kilodalton antigen from roundworm ascaris parasites are protected against larval migration of ascaris suum. | protective immunity to the pig roundworm, ascaris suum, has been demonstrated by immunization of pigs with antigens derived from the parasite's larval stages. we identified a protective antigen commonly expressed in the human and pig ascaris infections as a 16-kda protein (as16), which has no similarity at the amino acid level to mammalian proteins but has some similarity to those of the filarial parasites and caenorhabditis elegans gene product. localization analysis revealed that the native as ... | 2003 | 12933879 |
| [frequency of seropositivity to toxocara canis in children of different socioeconomic strata]. | frequency of seropositivity for toxocara in children from different socioeconomic strata in the city of brasilia (brazil) was measured. six hundred and two children of both sexes, aged one to 12 years were distributed in two socioeconomically distinct groups. the samples of sera of the first group were obtained from blood drawn for routine tests in the laboratory of a public hospital attending children from low-income families. samples from the second group were obtained from private laboratorie ... | 2003 | 12937730 |
| purification and characterization of acid trehalase from muscle of ascaris suum (nematoda). | acid trehalase (ec 3.2.1.28) was isolated from muscle of ascaris suum by fractionating with ammonium sulfate, acetone and column chromatography on deae-cellulose and phenyl sepharose cl-4b. the purified homogeneous preparation of native acid trehalase exhibited a molecular mass of 76 kda and of 38 kda on sds-page. the enzyme has the optimum ph 4.9, pi 4.3, km of 6.6 mm and vmax=34.5 nm min(-1) x mg(-1). besides trehalose, it hydrolyses sucrose, isomaltose and maltose and, to a lesser degree mele ... | 2003 | 12941639 |
| wild boar helminths: risks in animal translocations. | the helminth populations found in a group of wild boars collected in central spain were compared to those in a group of animals imported from a french game farm that produces boars for restocking. eleven helminth species, including ten nematodes and one acanthocephalan, were found. gongylonema pulchrum and macracanthorhynchus hirundinaceus were only detected in autochthonous wild boars, while oesophagostomum dentatum, ascaris suum, and trichuris suis were detected in imported animals only. autoc ... | 2003 | 12944047 |
| specific systemic igg1, igg2 and igm responses in pigs immunized with infective eggs or selected antigens of ascaris suum. | a total of 35 pigs aged 15 weeks old, and 21 pigs aged 8 weeks old were divided into 7 groups. groups 1 and 2 were uninfected and challenge control groups, respectively. groups 3 and 4 were infected weekly with 6 increasing doses of ascaris suum eggs, and group 4 was additionally treated with pyrantel. groups 5, 6, and 7 were immunized weekly with the 14, 42, or 97 kda fractions from adult worms, respectively. animals of groups 2-7 were challenged with 10000 a. suum eggs 7 days after the last in ... | 2003 | 12964832 |
| [effect of ethereal oils and of certain of their fractions on dehydrogenase activity in paramecium and on ascaris suum]. | | 1955 | 13256796 |
| the effect of piperazine-hcl on migrating larvae of ascaris suum goeze 1782. | | 1955 | 13259047 |
| the enhancement of virus pneumonia of pigs by the migration of ascaris suum larvae. | | 1957 | 13416082 |
| the influence of diet on the development of ascaris suum in the small intestines of pigs. | | 1958 | 13533764 |
| the affect of ascaris suum migration on the severity of swine influenza. | | 1958 | 13575318 |
| [studies on ascaris invasions in pigs. i. conditions favorable for incubation of ascaris suum goeze, 1782 in the external environment; preliminary communication]. | | 1958 | 13604198 |
| [studies on radio-sensitivity of ascaris lumbricoides and ascaris suum eggs and invasive capacity of developing larvae]. | | 1958 | 13622052 |
| the effect of hygromycin b on the migrating larvae of ascaris suum. | | 1959 | 13641085 |
| cyclooctadepsipeptides--an anthelmintically active class of compounds exhibiting a novel mode of action. | there are three major classes of anthelmintics for veterinary use: the benzimidazoles/prebenzimidazoles, the tetrahydropyrimidines/imidazothiazoles, and the macrocyclic lactones. in nematodes, there are five targets for the existing anthelmintics: the nicotinergic acetylcholine receptor which is the target of tetrahydropyrimidines/imidazothiazoles and indirectly that of the acetylcholineesterase inhibitors; the gaba receptor which is the target of piperazine, the glutamate-gated chloride channel ... | 2003 | 13678839 |
| [a contribution to the problem of the pathogenicity of ascaris suum for man]. | | 1961 | 13764621 |
| immunization of guineapigs against ascaris suum. | | 1960 | 13774786 |
| [effect of actinomycetes, fungi and bacteria isolated from the soil in areas irrigated with sewage on ascaris suum eggs]. | | 1959 | 13805793 |
| serum transaminase and aldolase during migration of larval ascaris suum in swine. | | 1961 | 13861497 |
| fluorescent antibody studies in vitro on ascaris suum goeze, 1782. | | 1962 | 13918915 |
| the in vitro action of immune pig serum on second and third-stage ascaris suum larvae. | | 1961 | 13918917 |
| the passive transfer of immunity in guinea-pigs infected with the nematode ascaris suum goeze, 1782. | | 1961 | 13918918 |
| [on the physiological basis of the rhythmic muscle activity of ascaris suum goeze]. | | 1962 | 13935754 |
| [studies on the antigenic structure of the eggs of ascaris suum. i]. | | 1962 | 13939151 |
| [proteins in the cavitary fluid of ascarides in swine (ascaris suum goeze, 1758) in conditions of maintaining the parasites on a protein-free diet]. | | 1962 | 13967392 |
| [further study on chemotherapeutic action on ascaris suum larvae during their migratory stage]. | | 1963 | 13972864 |
| trypsin and chymotrypsin inhibitors from ascaris suum. | | 1963 | 13981974 |
| cuticular lesions of ascaris suum caused by pseudomonas sp. | | 1963 | 13984127 |
| [properties of free amino acids in cavitary fluids of ascarides in swine (ascaris suum goeze, 1782) and their change in cultivation of the ascarides in conditions of protein deficiency]. | | 1962 | 13991135 |
| in vitro uptake of co60-vitamin b12 by ascaris suum. | | 1963 | 14003145 |
| larvicidal activity of dithiazanine iodide administered in feed to pigs with artificial infections of ascaris suum. | | 1963 | 14015696 |
| the effects of different atmospheres and various supplements on the in vitro survival and growth of ascaris suum larvae. | | 1963 | 14021657 |
| immunological studies on experimental infection of pigs with ascaris suum goeze, 1782. i. an introduction with a review of the literature and the demonstration of complement-fixing antibodies in the serum. | | 1961 | 14039611 |
| trypsin and chymotrypsin inhibitors from ascaris suum. | | 1963 | 14042869 |
| [effect of the preparation "vapam" on ascaris lumbricoides and ascaris suum eggs in vitro]. | | 1963 | 14051080 |
| immunological studies on experimental infection of pigs with ascaris suum goeze, 1782. ii. the use of the conglutinating complement absorption test for the demonstration of ascaris antibody. | | 1963 | 14054391 |
| in vitro fluorescent antibody studies on ascaris lumbricoides and ascaris suum. | | 1963 | 14062271 |
| immunological studies on experimental infection of pigs with ascaris suum goeze, 1782. iii. the antibody response and acquired immunity. | | 1964 | 14125098 |
| immunological studies on experimental infection of pigs with ascaris suum goeze, 1782. iv. the primary antibody response. | | 1964 | 14125099 |
| immunological studies on experimental infection of pigs with ascaris suum goeze, 1782. v. the antibody response to the oral administration of third- and fourth-stage larvae. | | 1964 | 14125100 |
| [mitochondria in muscle tissue of ascaris suum goeze, 1758. i. preparation of an isolated suspension and study of the oxidative activity]. | | 1963 | 14159556 |
| [on the possibility of infecting man with ascaris suum]. | | 1964 | 14167011 |
| electron microscope studies on the intestinal epithelium of ascaris suum. | | 1964 | 14169530 |
| acquired immunity to migrating larvae of ascaris suum induced in pigs by repeated oral inoculations in infective eggs. | | 1964 | 14206469 |
| studies in helminth enzymology. 3. malic dehydrogenases of ascaris suum. | | 1964 | 14213221 |
| in vivo studies of ascaris suum larvae planted in diffusion chambers in immune and nonimmune mice. | | 1964 | 14215493 |
| [effect of gibberelin on the embryonal development of ascaris suum]. | | 1964 | 14219162 |
| fluorescent antibody staining of ascaris suum larvae in the liver and lung of an infected guinea-pig by means of the freeze-substitution technique. | | 1964 | 14249020 |
| immunological studies on experimental infection of guineapigs and rabbits with ascaris suum goeze, 1782. i. the minimum immunizing dose (m.i.d.) of ascaris eggs in guineapigs. | | 1964 | 14250817 |
| immunological studies on experimental infection of guineapigs and rabbits with ascaris suum goeze, 1782. ii. antibody production and the further demonstration of acquired resistance in guineapigs. | | 1964 | 14250818 |
| immunological studies on experimental infection of guineapigs and rabbits, with ascaris suum goeze, 1782. 3. the antibody response in rabbits demonstrated by means of the complement fixation and conglutinating complement absorption tests. | | 1964 | 14250819 |
| trehalose synthesis and hydrolysis in ascaris suum. | | 1965 | 14259486 |
| [muscle tissue mitochondria of ascaris suum goeze, 1758. ii. effect of alcopar and some of its derivatives on the oxidation in various krebs cycle components]. | | 1964 | 14321580 |