| [experimental infection in mastomys natalensis (smith 1834) with trypanosoma species of the lewisi-congolense and brucei-evansi groups]. | | 1971 | 4946451 |
| ciliated fibroblasts in rabbit ear chambers. | | 1971 | 4947889 |
| [demonstration of antibodies and quantitative determination of serum igm in experimental trypanosomiasis of the guinea pig]. | | 1971 | 5003797 |
| [immunotaxonomic interrelations between t. brucei, t. rhodesiense, and t. gambiense]. | | 1971 | 5005128 |
| [culture of trypanosoma brucei in the biphasic media with human and animal blood]. | | 1971 | 5005863 |
| bovine trypanosomiasis and lymphocytosis parallel studies. | | 1970 | 5006224 |
| immunosuppression during trypanosomiasis. | | 1972 | 5014242 |
| trypanosoma brucei: fluxes of the morphological variants in intact and x-irradiated mice. | | 1972 | 5016599 |
| the potential for physiological variation in strains of trypanosoma evansi in relation to the state of the kinetoplast. | | 1972 | 5021571 |
| the febrile response to treatment in gambian sleeping sickness. | | 1972 | 5021574 |
| reduction in the ability of trypanosoma brucei to react with antibodies after long exposure to immune mechanisms in vivo. | | 1972 | 5022505 |
| isolation, partial purification, and properties of thymidine kinase from trypanosoma (trypanozoon) brucei rhodesiense. | | 1972 | 5022867 |
| the cryopreservation of trypanosoma brucei in polythene tubing. | | 1972 | 5045628 |
| trypanosoma brucei brucei: polycations and the release of alanine aminotransferase. | | 1972 | 5046890 |
| trypanosoma brucei in the haemoceole of glossina. | | 1972 | 5048098 |
| experiences with glossina morsitans from the langford colony during trypanosome infection experiments with tsetse flies in tororo, uganda. | | 1972 | 5048801 |
| a high rate of infection of wild glossina fuscipes with trypanosoma brucei subgroup trypanosome. | | 1972 | 5048832 |
| developmental changes in ultrastructure and physiology of trypanosoma brucei. | | 1972 | 5048840 |
| factors influencing the development of trypanosomes in tsetse flies. | | 1972 | 5048842 |
| the establishment and initial survival of trypanosoma brucei in the gut of glossina pallidipes. | | 1972 | 5048843 |
| a histochemical investigation of fibrin deposits in the tissues of rabbits infected with trypanosoma brucei. | | 1972 | 5048847 |
| investigation of changes in the physiology of the cerebrospinal fluid in rabbits infected with trypanosoma brucei. | | 1972 | 5048848 |
| trypanosoma brucei infections in mice: studies on the anaemia. | | 1972 | 5048849 |
| non-trypanosome specific components of the elevated igm levels in rabbit trypanosomiasis. | | 1972 | 5048853 |
| sugar composition of trypanosome brucei subgroup 4s (surface) antigens. | | 1972 | 5048855 |
| immunization of cattle with x-irradiated african trypanosomes. | | 1972 | 5048857 |
| the antigenicity of the alanine aminotransferase of trypanosome brucei brucei. | | 1972 | 5048858 |
| the satellite dna's of salivarian trypanosomes. | | 1972 | 5048860 |
| observations on the isolation, purification and partial characterization of high molecular weight satellite dna from trypanosoma brucei. | | 1972 | 5048861 |
| trypanosomal meningo-encephalomyelitis with localization of t. brucei in the brain of a dog. | | 1972 | 5048863 |
| indirect haemagglutination test with sera of animals experimentally infected with trypanosoma brucei and t. rhodesiense. | | 1972 | 5050539 |
| a high infection rate of trypanosoma brucei subgroup in clossina fuscipes. | | 1972 | 5053038 |
| influence of ambient temperature on the course of experimental trypanosomiasis in mice. | | 1972 | 5063170 |
| purification of the 4s antigens of brucei subgroup trypanosomes. | | 1972 | 5065571 |
| multiplication of blood-stream forms of trypanosoma brucei in vitro at 37 degrees c. in the presence of mouse blood. | | 1972 | 5071049 |
| inhibition of cyanide insensitive respiration in trypanosoma brucei. | | 1972 | 5071071 |
| cryostorage in polythene tubing. | | 1972 | 5071073 |
| development of trypanosoma brucei in the gut of glossina morsitans. | | 1972 | 5071084 |
| lysosomes in trypanosoma brucei. | | 1972 | 5071085 |
| detection of low concentrations of trypanosomes in blood by column-separation and membrane-filtration. | | 1972 | 5071091 |
| trypanosoma brucei infection in the haemocoel of tsetse flies. | | 1972 | 5071092 |
| the influence of type of diet on trypanosoma brucei infection in rats. | | 1972 | 5071723 |
| cryopreservation of pathogenic african trypanosomes in situ: metacyclic and bloodstream forms. | | 1972 | 5074424 |
| the indirect haemagglutination test in the study of trypanosoma vivax infections of sheep. | | 1971 | 5096375 |
| the lactic dehydrogenase activity of trypanosomes and platelets isolated from ray rat blood. | | 1971 | 5120308 |
| relationship between the species vectors of tsetse flies and the pathogenicity and antigenic characteristics of the brucei-group trypanosomes. | | 1971 | 5138873 |
| the effect of suramin on blood and tissue forms of trypanosoma brucei and trypanosoma rhodesiense. | | 1971 | 5145111 |
| experimental infections of the chimpanzee (pan troglodytes) with trypanosoma brucei brucei and trypanosoma brucei rhodesiense. | | 1971 | 5145112 |
| cell-mediated hypersensitivity in rabbits infected with trypanosoma brucei and trypanosoma rhodesiense. | animals infected with strains of trypanosoma brucei and t. rhodesiense exhibited cutaneous hypersensitivity to intradermal administration of antigen. this reactivity was of two types, an arthus-type, antibody-mediated reaction which reached maximum intensity 4 hr after injection and a delayed-type, cell-mediated reaction which reached maximum intensity 24 hr after injection. this delayed-type hypersensitivity appeared in rabbits not earlier than 3 weeks after onset of infection. it did not occur ... | 1971 | 5154901 |
| the occult visceral phase of mammalian trypanosomes with special reference to the life cycle of trypanosoma (trypanozoon) brucei. | | 1971 | 5157436 |
| an amastigote phase of the sleeping sickness trypanosome. | | 1971 | 5157437 |
| the propagation and survival of trypanosoma brucei in vitro at 37 degrees c. | | 1971 | 5159142 |
| the use of human plasma in the blood incubation infectivity test to differentiate trypanosoma brucei and trypanosoma rhodesiense. | | 1971 | 5159409 |
| clinicopathological study on experimental trypanosma brucei infections in horses. 2. histopathological findings in the nervous system and other organs of treated and untreated horses reacting to nagana. | | 1971 | 5164810 |
| clinicopathological study on experimental trypanosoma brucei infections in horses. 1. development of clinically recognizable nervous symptoms in nagana-infected horses treated with subcurative doses of antrypol and berenil. | | 1971 | 5164811 |
| relationship between eperythrozoon coccoides and trypanosoma (trypanozoon) brucei brucei in experimentally infected mice. | | 1970 | 5500106 |
| topological analysis of antigenic determinants on a variant surface glycoprotein of trypanosoma brucei. | nine monoclonal antibodies specific for the variant surface glycoprotein (vsg) of trypanosoma brucei mitat 1.6 have been produced and characterised as part of a coordinated project to define the three dimensional structure and immunological profile of this and other vsg molecules. competition radioimmunoassays using all combinations of these antibodies identified five distinct antigenic determinants, showing varying degrees of overlap, on the mitat 1.6 vsg molecule. a map has been constructed of ... | 1984 | 6083452 |
| the istat 1.3 vsg multigene family in trypanosoma brucei: retention of the expression linked copy through multiple antigenic switches. | in the istar 1 serodeme of t. brucei the 3 variant surface glycoprotein (vsg) gene family contains about 10 members, one of which has a telomeric location on a minichromosome. the expression linked copy (elc) of the 3 vsg gene which occurs in an antigenic variant expressing the 3 vsg, also has a telomeric location but unlike the minichromosomal 3 vsg gene has restriction sites upstream from the 5' barren region. this elc is retained on the same telomere in a subsequent variant that expresses a t ... | 1984 | 6083548 |
| mapping of antigenic determinants within peptides of a variant surface glycoprotein of trypanosoma brucei. | the location of antigenic determinants in the primary amino acid sequence of the variant surface glycoprotein of trypanosoma brucei mitat 1.6 was investigated using monoclonal antibodies in conjunction with the known cyanogen bromide and tryptic cleavage patterns of this antigen. the cyanogen bromide digestion fragments of the antigen were purified and used to raise polyclonal antisera, which were specific for the appropriate cyanogen bromide fragment and partial digestion products, as well as r ... | 1984 | 6084814 |
| the inactivation and reactivation of an expression-linked gene copy for a variant surface glycoprotein in trypanosoma brucei. | we have previously shown that the gene for variant surface glycoprotein 118 of trypanosoma brucei (strain 427) is activated by a duplicative transposition to a telomeric expression site. in chronically-infected animals, this expression-linked copy is lost when the 118 gene is replaced at the expression site by another variant surface glycoprotein gene. we show here that expression of the 118 gene can also be switched off without loss of the extra expression-linked copy. in two variants, called 1 ... | 1984 | 6086319 |
| possible dna modification in gc dinucleotides of trypanosoma brucei telomeric sequences; relationship with antigen gene transcription. | polymorphism in restriction site cleavage (psti, sphi, pvuii, hindiii) has been noticed in several occasions in the telomeric sequences harbouring trypanosome variant-specific antigen genes (1, 2, 3). this polymorphism has been further investigated and seems best interpreted as due to partial dna modification in gc dinucleotides. the actively transcribed telomeric genes do not exhibit such a polymorphism; furthermore, in at least three independent cases, gene inactivation is linked to the appear ... | 1984 | 6087278 |
| trypanosome mrnas share a common 5' spliced leader sequence. | a 5'-terminal leader sequence of 35 nucleotides was found to be present on multiple trypanosome rnas. based on its representation in cdna libraries, we estimate that many, if not all, trypanosome mrnas contain this leader. this same leader was originally identified on mrnas encoding the molecules responsible for antigenic variation, variant surface glycoproteins. studies of selected cdnas containing this leader sequence revealed that leader-containing transcripts can be stage-specific, stage-reg ... | 1984 | 6088073 |
| apparent discontinuous transcription of trypanosoma brucei variant surface antigen genes. | the repeated mini-exon sequence that encodes the first 35 base pairs of all variant surface antigen mrnas of trypanosoma brucei directs the synthesis of a discrete 137-nucleotide transcript. it thus seems that variant surface antigen mrnas are transcribed discontinuously, and we present two alternative models for how this might occur. | 1984 | 6090933 |
| identification of a small rna containing the trypanosome spliced leader: a donor of shared 5' sequences of trypanosomatid mrnas? | the 35 nucleotide spliced leader (sl) sequence is found on the 5' end of numerous trypanosome mrnas, yet the tandemly organized reiteration units encoding this leader are not detectably linked to any of these structural genes. here we report the presence of a class of discrete small sl rna molecules that are derived from the genomic sl reiteration units of trypanosoma brucei, trypanosoma cruzi, and leptomonas collosoma. these small sl rnas are 135, 105, and 95 nucleotides, respectively, and cont ... | 1984 | 6091897 |
| antigenic variation in african trypanosomes by gene replacement or activation of alternate telomeres. | we have analyzed antigenic variants with a known lineage and show that there are several telomeres on which variant surface glycoprotein (vsg) genes can be expressed. these telomeres have similar restriction maps 5' to the barren region. in addition, the same vsg gene was expressed on different telomeres. some antigenic switches in the lineage were accomplished by duplicative replacement of one vsg gene with another. other switches occurred without duplication by transcriptional activation of an ... | 1984 | 6091912 |
| the sequence of the gene for cytochrome c oxidase subunit i, a frameshift containing gene for cytochrome c oxidase subunit ii and seven unassigned reading frames in trypanosoma brucei mitochondrial maxi-circle dna. | a 9.2 kb segment of the maxi-circle of trypanosoma brucei mitochondrial dna contains the genes for cytochrome c oxidase subunits i and ii (coxi and coxii) and seven unassigned reading frames ("urfs"). the genes for coxi and coxii display considerable homology at the aminoacid level (38 and 25%, respectively) to the corresponding genes in fungal and mammalian mtdna, the only striking point of divergence being an unusually high cysteine content (about 4.5%). the reading frame coding for cytochrome ... | 1984 | 6093040 |
| translocation alters the activation rate of a trypanosome surface antigen gene. | we report here the characterization of the gene coding for antat 1.13, a very late variable antigen type (vat) from trypanosoma b. brucei. this gene is chromosome-internal and it is activated by the duplicative mechanism. like in another case of late vat expression (1), its expression-linked copy (elc) is flanked by "companion" sequences. it was possible to convert the late expression of this vat into an early one, by changing the location of the gene in the genome. this has been achieved by sel ... | 1984 | 6095196 |
| sequences of six genes and several open reading frames in the kinetoplast maxicircle dna of leishmania tarentolae. | the dna sequence of approximately 80% of the transcribed region of the kinetoplast maxicircle dna of leishmania tarentolae was obtained, and structural genes were localized by comparison of the translated amino acid sequences with those of known mitochondrial genes from other organisms. by this method, the genes for cytochrome oxidase subunits i, ii, and iii, cytochrome b, and human mitochondrial unidentified reading frames 4 and 5 were identified. by comparing the amino acid sequences of the pu ... | 1984 | 6096360 |
| responsiveness of the rat vas deferens to catecholamines and electrical stimulation during an infection with trypanosoma brucei. | the effect of an acute infection with trypanosoma brucei on the reactivity of the rat vas deferens to noradrenaline, tyramine and field stimulation was studied. the vas deferens isolated from infected animals was more responsive to field stimulation than control rat vas deferens. the effect was more noticeable at lower frequencies of stimulation suggesting an increased sympathetic discharge from presynaptic nerve endings. also both noradrenaline and tyramine were more effective on the infected v ... | 1984 | 6099973 |
| activation of trypanosome surface glycoprotein genes involves a duplication-transposition leading to an altered 3' end. | expression of the genes for variant surface glycoproteins 117 and 118 in trypanosoma brucei is accompanied by the appearance of an extra copy of these genes, the expression-linked copy, which differs in the surrounding restriction enzyme sites from the corresponding basic copy of the genes. we present direct evidence that the expression-linked copy is the one used for messenger rna synthesis. by s1-nuclease-protection experiments we show that cloned basic-copy genes contain the nucleotide sequen ... | 1981 | 6101223 |
| antigenic variation in clones of animal-infective trypanosoma brucei derived and maintained in vitro. | eighteen clones of variable antigen type 052 of trypanosoma brucei stock s.427 were derived and maintained as animal-infective bloodstream forms in vitro for up to 60 days of cultivation. the antigenic composition of such clones was monitored weekly by immunofluorescent analysis of viable trypanosomes, using antisera raised to isolated variant-specific surface glycoproteins of both 052 and variable antigen type (221) which consistently appeared in the first relapse population of type 052 in vitr ... | 1980 | 6102753 |
| colonization of trypanosoma brucei gambiense within transplanted ehrlich's tumors of microtus montanus. | trypanosoma brucei gambiense has been observed growing in extravascular sites throughout a solid ehrlich ascites tumor (eat) in microtus montanus. these trypanosomes appear in clusters in the tumor. the possible importance of this observation to the host-parasite relationship is discussed. | 1980 | 6104421 |
| detection and quantification of variant specific antigen in the plasma of rats and mice infected with trypanosoma brucei brucei. | variant specific antigen (vsa), the principal constituent of the surface coat of salivarian trypanosomes, was detected by gel immunoassays in the plasma of rats and mice infected with trypanosoma brucei brucei. the quantity of vsa in plasma was measured in radial immunodiffusion tests using a monospecific antiserum and purified vsa as a standard. during the first peak of parasitemia, a statistically significant, linear relationship was determined between the number of parasites in the blood (in ... | 1980 | 6104691 |
| the sequential cellular changes in the local skin reaction produced in goats by glossina morsitans morsitans infected with trypanosoma (trypanozoon) brucei. | sequential biopsies of the skin reaction elicited in goats by glossina morsitans morsitans infected with trypanosoma (trypanozoon) brucei were examined histologically to identify and to quantify the cellular populations involved in the reaction. the peak of the tissue response occurred 7-8 days after challenge with infected tsetse and preceded the initial detection of parasitaemia by 4-5 days. microscopically, the cellular reaction was characterized initially by a marked infiltration of polymorp ... | 1980 | 6106349 |
| mechanoreceptor--trypanosome interactions in the labrum of glossina: fluid mechanics. | the fluid mechanics of the flow of blood through the labrum of glossina is described in uninfected and trypanosome infected flies. the flow is characterised by the reynolds number, and a frequency parameter for the pulsating flow and non-newtonian viscosity effects are considered. the effects of colonies of trypanosomes on the flow rate in the labrum and the interactions between colonies of trypanosoma (nannomonas) congolense and trypanosoma (trypanozoon) brucei and mechanoreceptors in the proxi ... | 1980 | 6106350 |
| studies on the mechanisms supporting the continuous growth of trypanosoma (trypanozoon) brucei as bloodstream-like form in vitro. | the conditions which enable growth of trypanosoma (t.) brucei stock s 427 as bloodstream-like form over bovine fibroblast-like cells (bhf) were analyzed. the experiments with different bhf conditioned media indicated that there is no stable factor produced by the bhf which can trigger growth as bloodstreamlike form. only living, actively metabolizing bhf could support continuous growth. the intercellular localization of trypanosomes in the bhf feeder-layer was a main feature of an intact culture ... | 1980 | 6106356 |
| an improved method for the estimation of suramin in plasma and trypanosome samples. | an improved method for the chemical estimation of suramin is described in which the aromatic amines released from the drug by acid hydrolysis are diazotised and then coupled to n-(1-naphthyl)-ethylenediamine to form a pink coloured product (emm545nm267 /+- 5) provided certain precautions are followed, the assay method is highly reproducible (+/- 2%) and sufficiently sensitive to measure 2.5 nmol; suramin in mixtures with plasma (0.5 ml) or trypanosomes (200 mg wet wt.). | 1980 | 6108509 |
| uptake of the trypanocidal drug suramin by bloodstream forms of trypanosoma brucei and its effect on respiration and growth rate in vivo. | after a single intravenous injection of suramin the rate of removal of the drug from the plasma into other tissue compartments of the rat is independent of initial concentration. the data can be fitted to the sum of two exponential functions, consistent with a two-compartment, open model system. trypanosomes take up only small amounts of suramin in vivo and do not actively concentrate the drug within the cell. uptake is apparently by a non-saturable process that decreases with time and is depend ... | 1980 | 6108510 |
| trypanosoma brucei: host parasite interaction in parasite destruction by salicylhydroxamic acid and glycerol in mice. | | 1981 | 6109641 |
| attachment of trypanosoma brucei to rabbit peritoneal exudate cells. | | 1981 | 6112207 |
| macrophages as primary target cells and mediators of immune dysfunction in african trypanosomiasis. | african trypanosomiasis is accompanied by profound general immunosuppression. the experiments described here were designed to characterize the contribution of macrophages to the immune pathology of this disease. we used peptone-stimulated, uninfected mice and injected them intraperitoneally with lethally irradiated and 35s-labeled trypanosoma brucei and parasite-specific antisera. peritoneal macrophages were thus induced to take up in vivo a defined number of trypanosomes. after the phagocytosis ... | 1981 | 6114926 |
| infectivity of monomorphic and pleomorphic trypanosoma brucei stocks cultivated at 28 c with various tsetse fly tissues. | noninfective procyclic forms of trypanosoma brucei stocks derived from the pleomorphic eve 10 were cultivated at 28 c in cunningham's liquid medium in the presence of head-salivary gland, alimentary tract, and abdominal body wall explants of glossina morsitans morsitans. after 8 to 10 days of cultivation some of the procyclic forms transformed into metacyclic stages infective for mice. infectivity persisted for varying periods up to 66 days, when the experiments were terminated. only 10 explants ... | 1981 | 6115002 |
| variation in the sensitivity of successive variable antigen types in a trypanosoma (trypanozoon) brucei subspecies clone to some african game animal sera. | a trypanosoma brucei subspecies clone was passaged in rats at ten-day intervals and the sensitivity/resistance to a variety of mammalian sera, of the successive variable antigen types (vats) produced, was examined sequentially in a modified version of the blood incubation infectivity test (biit). a proven homogeneous vat was used to initiate this series of tests, in which seven successive and different vats were each exposed in vitro at 38.5 degrees c for 2 h to standard samples of pooled rat se ... | 1981 | 6115551 |
| resistance to trypanosoma brucei in mice following inoculation of ntg-treated trypanosomes. | the alkylating agent n-methyl-n'-nitro-n-nitrosoguanidine (ntg) was tested as an attenuating agent for trypanosoma brucei. parasites were treated with 4 mcg/ml ntg and injected into mice at intervals of 7-14 days. those mice surviving four injections of ntg-treated trypanosomes were later challenged and showed a 100% resistance to infection. a corresponding group of control mice showed a 6% resistance to infection. | 1981 | 6116437 |
| non-specific immune responses in cba/n mice infected with trypanosoma brucei. | cba/n mice carry a genetic defect which causes a maturational arrest of normal b lymphocyte development, and results in an inability to mount igm antibody responses against certain t lymphocyte-independent antigens. these mice were found to survive an experimental infection with trypanosoma brucei two to three times longer than conventional mice. this enhanced survival does not appear to be related to the level of infection of the host since parasitaemias were similar in all strains tested. in a ... | 1981 | 6117821 |
| surface coat variant antigen of trypanosoma brucei brucei: its clearance from blood and concentration in organs of normal, infected, and immune mice. | experiments were conducted to determine the fate of variant antigen once it was shed from the surface of trypanosoma brucei brucei. radioiodinated variant antigen was administered intravenously to normal mice, mice immunized to the homologous variant antigen, and mice infected with an antigenically dissimilar (heterologous) t. brucei brucei variant. the variant antigen was cleared slightly faster in infected and immune animals. though over 80% of the variant antigen was cleared in all animals wi ... | 1982 | 6119292 |
| biosynthesis of trypanosoma brucei variant surface glycoprotein. i. synthesis, size, and processing of an n-terminal signal peptide. | an n-terminal signal peptide has been identified on a precursor of the variant surface glycoprotein (vsg) of trypanosoma brucei, synthesised both in vitro and in vivo. an ordered cyanogen bromide (cnbr) cleavage map was constructed for both mature vsg and for precursor vsg, and this allowed a size estimate of the signal peptide to be made by comparison of the n-terminal cnbr peptides produced from each. the signal peptide contains 30-40 amino acids. analysis of the precursor vsgs produced when n ... | 1981 | 6120455 |
| trypanosoma brucei: a quick method for separating blood-stream trypomastigotes from infected blood by differential osmotic lysis. | 1. the degree of rat erythrocyte lysis and immobilization of trypanosoma brucei in infected blood by buffered hypotonic solutions of sodium chloride and sources was studied. 2. at 0.3% sodium chloride solution 98% hemolysis of erythrocytes was achieved while 95% of the original bloodstream trypomastigotes survived and were found to be motile and viable for biochemical study. 3. further increase in the concentration of sodium chloride above 0.3% revealed an increase in the immobilization of trypa ... | 1982 | 6121651 |
| regulation of glycolysis in trypanosoma brucei: hexokinase and phosphofructokinase activity. | the kinetic properties of the glycosomal hexokinase (hg)2 and phosphofructokinase (pfk) from trypanosoma brucei bloodstream forms were investigated. hexokinase has a very high affinity for glucose (km = 17 microm) and exhibits a broad ph optimum with a maximum at ph 7.8. no indications have been found for regulation of hk activity. phosphofructokinase behaves as an allosteric protein with respect to its substrate, fructose-6-phosphate. 5'-amp acts as a positive allosteric effector. the apparent ... | 1982 | 6122364 |
| the use of aerolysin toxin as an aid for visualization of low numbers of african trypanosomes in whole blood. | a method was developed for detection of low numbers of african trypanosomes in whole blood. the method is based on selective lysis of erythrocytes and leukocytes by aerolysin, a toxin produced by the bacterium aeromonas hydrophila. african trypanosomes do not bind the toxin and their viability and motility are therefore unaffected by this treatment. | 1982 | 6122365 |
| early course of infection in susceptible and resistant strains of mice, using [3h]uridine-labeled trypanosoma brucei subsp. brucei. | a radioisotopic technique utilizing [3h]uridine was developed to determine the tissue distribution of intravenously inoculated trypanosoma brucei spp. brucei in susceptible (swiss-webster) and resistant (deer) strains of mice. the reliability of the technique was tested by using unincorporated [3h]uridine and heat-killed (labeled) trypanosomes in parallel experiments. the inoculations with viable (labeled) organisms showed that during the initial 24 h t. brucei subsp. brucei was cleared from the ... | 1982 | 6123484 |
| in vivo and in vitro activity by diverse chelators against trypanosoma brucei brucei. | a system of prescreens and screen has been developed to select chelators as potential drugs against trypanosoma brucei brucei eatro 110. the chelators tested were nearly all commercially available, low molecular, and having moderate to high affinity for fe(iii). we prescreened 70 compounds showing heme-sparing or inhibitory activity in a crithidia fasciculata growth system having excess fe and minimal hemin. of these, 45 were highly trypanocidal for suspensions of bloodstream t. b. brucei; crite ... | 1982 | 6123596 |
| relapse of monomorphic and pleomorphic trypanosoma brucei infections in the mouse after chemotherapy. | infections in mice were initiated with trypomastigotes from two lines of trypanosoma brucei derived from the same primary isolated. infections with one line were initiated by inoculation of metacyclic trypomastigotes from infected tsetse flies and the resulting infections were pleomorphic. the other line had been passaged 32 times in rodents and inoculation of bloodstream trypomastigotes gave rise to monomorphic infections. in both infections there were high levels of parasitaemia until death up ... | 1982 | 6126053 |
| the biosynthesis of trypanosoma brucei variant surface glycoproteins--in vitro processing of signal peptide and glycosylation using heterologous rough endoplasmic reticulum vesicles. | the ability of stripped microsomal dog pancreas vesicles to process precursors of the variant glycoprotein of trypanosoma brucei has been investigated. the vesicles were shown to be capable of cleaving a peptide precursor variant surface glycoprotein, identified as the n-terminal signal peptide in the case of one variant. the vesicles were also shown to add carbohydrate to precursor variant surface glycoprotein. this carbohydrate, which was sensitive to the action of endo-h was located within a ... | 1982 | 6127629 |
| involvement of lysosomes in the uptake of macromolecular material by bloodstream forms of trypanosoma brucei. | to investigate whether the lysosomes of trypanosoma brucei are capable of uptake of macromolecules after internalization by the cell, we used triton wr-1339, a non-digestible macromolecular compound, which is known to cause a marked decrease in the density of hepatic lysosomes due to massive intralysosomal storage. intraperitoneal administration of 0.4 g/kg triton wr-1339 to rats infected with t. brucei led to the development of a large vacuole in the trypanosomes between nucleus and kinetoplast ... | 1982 | 6127630 |
| chemotherapy of trypanosoma brucei in mice with diminazene aceturate. variation in the aparasitaemic period over 5 years. | | 1982 | 6127848 |
| a new in vitro test for human serum resistance of trypanosoma (t.) brucei. | | 1982 | 6128897 |
| antitrypanosomal activity of sinefungin. | sinefungin, a naturally occurring antifungal antibiotic nucleoside containing an ornithine residue, linked by a c-c bond to c-5' of adenosine, cures mice infected with trypanosoma brucei brucei, t. congolense, or t. vivax; the effect of the drug is more pronounced towards t. congolense. anti-trypanosomal activity of sinefungin could be the result of the inhibition of transmethylation reactions or of polyamine biosynthesis--or both--in parasites. | 1983 | 6130709 |
| the transmission of mixed infections of pathogenic trypanosoma species to susceptible hosts by glossina morsitans morsitans. | | 1982 | 6131589 |
| a comparative evaluation of the parasitological techniques currently available for the diagnosis of african trypanosomiasis in cattle. | the parasitological techniques currently in use for the diagnosis of african trypanosomiasis were compared in a series of experiments for their capacity to detect trypanosoma congolense, t. vivax and t. brucei in the blood of cattle. the darkground/phase contrast buffy coat method proved to be more sensitive than the haematocrit centrifugation technique, thick, thin and wet blood films in detecting t. congolense and t. vivax. on the other hand with t. brucei, mouse inoculation was the most sensi ... | 1982 | 6131590 |