| optimization of cold methanol quenching for quantitative metabolomics of penicillium chrysogenum. | a sampling procedure for quantitative metabolomics in penicillium chrysogenum based on cold aqueous methanol quenching was re-evaluated and optimized to reduce metabolite leakage during sample treatment. the optimization study included amino acids and intermediates of the glycolysis and the tca-cycle. metabolite leakage was found to be minimal for a methanol content of the quenching solution (qs) of 40% (v/v) while keeping the temperature of the quenched sample near -20°c. the average metabolite ... | 2011 | 22833711 |
| statistical optimization of cellulases production by penicillium chrysogenum qml-2 under solid-state fermentation and primary application to chitosan hydrolysis. | solid-state fermentation conditions for cellulases production by a newly isolated penicillium chrysogenum qml-2 were investigated using statistical methods. at first, significant variables for cellulases production including (nh(4))(2)so(4), initial ph and inoculum size were screened by using plackett-burman design. then the optimal regions of the significant variables were investigated by using the method of steepest ascent. finally, central composite design and response surface analysis were a ... | 2011 | 22805837 |
| fleming's penicillin producing strain is not penicillium chrysogenum but p. rubens. | penicillium chrysogenum is a commonly occurring mould in indoor environments and foods, and has gained much attention for its use in the production of the antibiotic penicillin. phylogenetic analysis of the most important penicillin producing p. chrysogenum isolates revealed the presence of two highly supported clades, and we show here that these two clades represent two species, p. chrysogenum and p. rubens. these species are phenotypically similar, but extrolite analysis shows that p. chrysoge ... | 2011 | 22679592 |
| [influence of metal nanoparticles on some microorganisms and microflora of unpasteurized beer]. | the influence of nanoparticles of gold, silver, dioxide of cerium and zirconium in low concentration (0.5-7.5 mg/l) on pure cultures of escherichia coli iem-1, bacillus subtilis bt-2, candida scottii kb-2, saccharomyces cerevisiae ob-3, aspergillus niger r-3, fusarium culmorum t-7 and penicillium chrysogenum f-7 has been investigated. silver nanoparticles, which caused a decrease of the amount of s. cerevisiae ob-3 cells by 90% after one hour of exposition, and led to almost complete death of ve ... | 2011 | 22308746 |
| penicilliosis in an african grey parrot (psittacus erithacus). | penicillium mycosis, or penicilliosis, is rare in animals. this report describes the clinical signs and pathological, immunohistochemical, and biomolecular findings in a case of avian penicilliosis in a congo african grey parrot. on gross necropsy, pyogranulomatous changes were identified in the lung, liver, and kidney. histopathology demonstrated periodic acid schiff-(pas) and grocott-positive hyphae in these tissues. immunohistochemistry confirmed the genus of the fungus and polymerase chain r ... | 2011 | 22946411 |
| microbiota found in protective athletic mouthguards. | protective athletic mouthguards (pam) produce oral mucosal injuries and may be associated with other systemic conditions. | 2011 | 23016013 |
| comparative study on production, purification of penicillin by penicillium chrysogenum isolated from soil and citrus samples. | to explore various unexplored locations where penicillium spp. would be available and study the production of penicillin from the isolated penicillium spp. in different media with altered carbohydrate source. | 2011 | 23569718 |
| experimental and theoretical investigations of the adhesion time of penicillium spores to cedar wood surface. | in this study, the adhesion of 4 penicillium strains (penicillium granulatum, penicillium crustosum, penicillium commune and penicillium chrysogenum) on cedar wood was examined qualitatively and quantitatively by using the extended dlvo (xdlvo) approach and the environmental scanning electronic microscopy (esem) technique. a comparison between the xdlvo theories and the esem technique was also investigated. the adhesion tests revealed that p. chrysogenum was not able to adhere on the cedar wood ... | 2012 | 23827572 |
| new penicillin-producing penicillium species and an overview of section chrysogena. | species classified in penicillium sect. chrysogena are primary soil-borne and the most well-known members are p. chrysogenum and p. nalgiovense. penicillium chrysogenum has received much attention because of its role in the production on penicillin and as a contaminant of indoor environments and various food and feedstuffs. another biotechnologically important species is p. nalgiovense, which is used as a fungal starter culture for the production of fermented meat products. previous taxonomic st ... | 2012 | 23606767 |
| in vitro susceptibility of filamentous fungi to copper nanoparticles assessed by rapid xtt colorimetry and agar dilution method. | metal nanoparticles and their uses in various aspects have recently drawn a great deal of attention. one of the major applications is that it can be used as an antimicrobial agent. they can be considered in approaches targeted to decrease the harms caused by microorganisms, specifically fungi, threatening the medical and industrial areas. the aim of this study was to investigate the antifungal activity of synthesized copper nanoparticles (cunps) against four filamentous fungi including alternari ... | 2012 | 23518166 |
| antibacterial and antimycotic activities of slovenian honeys. | in the present study, slovenian honey samples produced from different floral sources are evaluated for their antibacterial and antifungal properties. the peroxide contribution to antibacterial activity is also determined. minimum inhibitory concentration (mic) of the honeys was assessed against four bacterial species (escherichia coli, enterococcus faecalis, pseudomonas aeruginosa and staphylococcus aureus) and against eight fungal species (aspergillus niger, aureobasidium pullulans, candida alb ... | 2012 | 23304790 |
| in vitro antifungal activity of dehydrozingerone and its fungitoxic properties. | the efficacy of dehydrozingerone (dz; dehydroderivative of zingerone) as an antifungal agent and its mode of action against food spoilage fungal pathogens was studied and presented. dz is a constituent of ginger (zingiber officinale rhizomes) and structural half analogue of curcumin. its efficacy against aspergillus oryzae, aspergillus flavus, aspergillus niger, aspergillus ochraceus, fusarium oxysporum and penicillium chrysogenum was evaluated. effect of dz on the growth and sporulation of a. o ... | 2012 | 23278709 |
| members of the penicillium chrysogenum velvet complex play functionally opposing roles in the regulation of penicillin biosynthesis and conidiation. | a velvet multisubunit complex was recently detected in the filamentous fungus penicillium chrysogenum, the major industrial producer of the β-lactam antibiotic penicillin. core components of this complex are p. chrysogenum vela (pcvela) and pclaea, which regulate secondary metabolite production, hyphal morphology, conidiation, and pellet formation. here we describe the characterization of pcvelb, pcvelc, and pcvosa as novel subunits of this velvet complex. using yeast two-hybrid analysis and bim ... | 2012 | 23264641 |
| evaluation of aerial microbial pollutants in al-haram al-nabawi during pilgrimage of 2013. | al-madinah al-munawwarah is the second holiest site in islam. the possibility of new emerging microbes is valid due to the increased number of pilgrims. the objectives of the current study were to estimate the numbers of fungi and bacteria inside and outside al-haram al-nabawi and to find whether new bacterial and fungal species have emerged compared to previous studies. air samples were collected twice a day from 12 spots and four directions during the pilgrim year of 2013 for four consecutive ... | 2015 | 28053593 |
| selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (gtg)5-pcr fingerprinting. | the aim of the study was a detail evaluation of genetic diversity among the lactic acid bacteria (lab) strains having an advantage of a starter culture in order to select genotypically diverse strains with enhanced antimicrobial effect on some harmfull and pathogenic microorganisms. antimicrobial activity of lab was performed by the agar well diffusion method and was examined against the reference strains and foodborne isolates of bacillus cereus, listeria monocytogenes, escherichia coli, staphy ... | 2014 | 26139877 |
| single-step fermentative production of the cholesterol-lowering drug pravastatin via reprogramming of penicillium chrysogenum. | the cholesterol-lowering blockbuster drug pravastatin can be produced by stereoselective hydroxylation of the natural product compactin. we report here the metabolic reprogramming of the antibiotics producer penicillium chrysogenum toward an industrial pravastatin production process. following the successful introduction of the compactin pathway into the β-lactam-negative p. chrysogenum ds50662, a new cytochrome p450 (p450 or cyp) from amycolatopsis orientalis (cyp105as1) was isolated to catalyz ... | 2015 | 25691737 |
| evaluation of a parchment document, the 13th century incorporation charter for the city of krakow, poland, for microbial hazards. | the literature of environmental microbiology broadly discusses issues associated with microbial hazards in archives, but these publications are mainly devoted to paper documents. there are few articles on historical parchment documents, which used to be very important for the development of literature and the art of writing. these studies present a broad spectrum of methods for the assessment of biodeterioration hazards of the parchment document in question. they are based on both conventional m ... | 2016 | 26896133 |
| comparative sequence analysis and mutagenesis of ethylene forming enzyme (efe) 2-oxoglutarate/fe(ii)-dependent dioxygenase homologs. | ethylene is one of the most used chemical monomers derived from non-renewable sources and we are investigating the possibility of producing it in yeast via the ethylene forming enzyme (efe) from pseudomonas syringae. to enable engineering strategies to improve the enzyme, it is necessary to identify the regions and amino acid residues involved in ethylene formation. | 2014 | 25278273 |
| functional characterization of a penicillium chrysogenum mutanase gene induced upon co-cultivation with bacillus subtilis. | microbial gene expression is strongly influenced by environmental growth conditions. comparison of gene expression under different conditions is frequently used for functional analysis and to unravel regulatory networks, however, gene expression responses to co-cultivation with other microorganisms, a common occurrence in nature, is rarely studied under laboratory conditions. to explore cellular responses of the antibiotic-producing fungus penicillium chrysogenum to prokaryotes, the present stud ... | 2014 | 24884713 |
| a natural anopheles-associated penicillium chrysogenum enhances mosquito susceptibility to plasmodium infection. | whereas studies have extensively examined the ability of bacteria to influence plasmodium infection in the mosquito, the tripartite interactions between non-entomopathogenic fungi, mosquitoes, and plasmodium parasites remain largely uncharacterized. here we report the isolation of a common mosquito-associated ascomycete fungus, penicillium chrysogenum, from the midgut of field-caught anopheles mosquitoes. although the presence of pe. chrysogenum in the anopheles gambiae midgut does not affect mo ... | 2016 | 27678168 |
| species-specific pcr to describe local-scale distributions of four cryptic species in the penicillium chrysogenum complex. | penicillium chrysogenum is a ubiquitous airborne fungus detected in every sampled region of the earth. owing to its role in alexander fleming's serendipitous discovery of penicillin in 1928, the fungus has generated widespread scientific interest; however its natural history is not well understood. research has demonstrated speciation within p. chrysogenum, describing the existence of four cryptic species. to discriminate the four species, we developed protocols for species-specific diagnostic p ... | 0 | 24179477 |
| penicillium chrysogenum dsoa associated with marine sponge (tedania anhelans) exhibit antimycobacterial activity. | a strain of penicillium chrysogenum was isolated from tedania anhelans (marine sponge) collected from indian ocean (8°22'30″n latitude and 76°59'16″ longitude) and deposited in culture collection centers. the strain subjected to different culture conditions for production of extrolites were extracted using ethyl acetate and chloroform. when both extracts were subjected for antibacterial activity, latter had high activity. minimum inhibitory concentration of chloroform extract ranged from 31.25-1 ... | 2016 | 26717859 |
| efficient gene targeting in penicillium chrysogenum using novel agrobacterium-mediated transformation approaches. | the industrial production of β-lactam antibiotics by penicillium chrysogenum has increased tremendously over the last decades, however, further optimization via classical strain and process improvement has reached its limits. the availability of the genome sequence provides new opportunities for directed strain improvement, but this requires the establishment of an efficient gene targeting (gt) system. recently, mutations affecting the non-homologous end joining (nhej) pathway were shown to incr ... | 2013 | 23994321 |
| synthesis and antimicrobial properties of 4-acylaminobenzenethiosulfoacid s-esters. | a series of esters of 4-acetyl, 4-trifluoroacetyl- and 4-(3-chloropropionyl)aminobenzenethiosulfoacids (twenty-four compounds) were synthesized and characterized by elemental analysis, (1)h nmr and ir spectroscopy. the antibacterial activity of the novel candidates has been screened using the agar diffusion or serial dilution methods against representative gram-positive (staphylococcus aureus, bacillus subtilis, bacillus mesentericus, mycobacterium sp., mycobacterium luteum), gram-negative (aero ... | 2017 | 28344478 |
| specific antibodies for the detection of alternaria allergens and the identification of cross-reactive antigens in other fungi. | the mould alternaria alternata is an important source of respiratory allergens. a. alternata extracts show great variations regarding allergenic potency. the aim of this study was to generate antibody probes specific for important alternaria allergens and to use them to study allergen expression, depending on different culture conditions, as well as to search for cross-reactive allergens in other mould species. | 2016 | 27780168 |
| how to diagnose mould allergy? comparison of skin prick tests with specific ige results. | diagnosis of mould allergy is complicated due to the heterogeneity of the test material and the decrease in the number of commercial mould skin test solutions that are currently available. | 2016 | 27027397 |
| influence of the silver nanoparticles on microbial community in different environments. | the aim of this study was to assess the influence of silver nanoparticles (agnps) on the number and diversity of microbial community in different environments (soil extract, water, sewage), and to determine whether the environment inhibits or accelerates the influence of agnps on moulds. agnps (45 ppm) present in the environment decreased bacterial (91%) and fungal (33-85%) numbers, and eliminated some strains, e.g., alternaria alternata and cryptococcus laurentii. based on the biomass growth of ... | 2015 | 26610310 |
| common airborne fungi induce species-specific effects on upper airway inflammatory and remodelling responses. | whilst the exact cause of chronic rhinosinusitis (crs) remains elusive, it is clear that both inflammation and remodelling are key disease processes. environmental fungi have been linked to airway inflammation in crs; however, their role in the pathogenesis of this condition remains controversial. the current consensus suggests that whilst fungi may not be directly causative, it is likely that crs patients have deficits in their innate and potentially acquired immunity, which in turn may modify ... | 2016 | 26501134 |
| the effect of saprotrophic fungi on the development and hatching of fasciola hepatica eggs. | the aim of this study was to determine the effect of 6 common soil fungi species: alternaria alternata (fr.) keissl., aspergillus candidus link, penicillium chrysogenum thom, p. commune thom, trichothecium roseum (pers.) link and ulocladium sp., on the hatching of miracidia, i.e., free living larvae of liver fluke (fasciola hepatica). to this end, the eggs of f. hepatica were incubated in water in the presence of one of the aforementioned fungi species and in tap water (control) at a temperature ... | 2014 | 25134345 |
| identification accuracy and diversity reproducibility associated with internal transcribed spacer-based fungal taxonomic library preparation. | this study investigated analytical parameters that are inherently relevant to identifying and quantifying fungal communities based on polymerase chain reaction amplicons. specifically, we evaluated the accuracy of the blastn-based identification for internal transcribed spacer (its) sequences generated from pure cultures, and quantified the reproducibility of relative abundances as well as α and β diversity measurements using duplicated environmental samples. the blastn-based method produced acc ... | 2014 | 24237760 |
| biochemical and immunological analysis of mould skin prick test solution: current status of standardization. | sensitization prevalence to moulds reached from less than 10% in the general population to more than 25% in atopic and/or asthmatic subjects. to diagnose ige-mediated mould sensitization, skin prick test (spt) and specific ige (sige) measurement are recommended. however, concordance of spt and sige results is often less than 50% and standardization of the extracts is required to achieve reliable test results. | 2013 | 24152161 |
| in vitro antioxidant and antifungal properties of essential oils obtained from aromatic herbs endemic to the southeast of spain. | the aim of this work was to determine (i) the antioxidant capacity of thymus moroderi, thymus piperella, santolina chamaecyparissus, and sideritis angustifolia essential oils (eos) by means of four different antioxidant tests (the 2,2'-diphenyl-1-picrylhydrazyl radical scavenging method, the ferrous ion-chelating ability assay, the ferric reducing antioxidant power test, and the thiobarbituric acid reactive species test) and (ii) the antifungal activity against molds and yeast of these eos by th ... | 2013 | 23834797 |
| fungal food choices of dermatophagoides farinae affect indoor fungi selection and dispersal. | house dust mite (hdm) feces and molds are the main allergens involved in allergic asthma. differences exist between the housing fungal biome of allergic patients and standard or unhealthy housing. house dust mite (hdm) feed off spores and transport them on their bodies, but do they have fungal food preferences? we observed dermatophagoïdes farinae in vitro with 16 mold menus and repeated the experiment 10 times. this observation led us to define alternaria alternata, cladosporium sphaerospermum, ... | 2013 | 22774849 |
| occupational exposure to airborne fungi in two croatian sawmills and atopy in exposed workers. | airborne fungi were collected over a one year period at 2-month intervals at 2 sawmills in croatia (sm 1 and sm 2) processing mainly beech wood and oak wood. a questionnaire concerning respiratory symptoms and skin prick test (spt) with common inhalatory allergens and moulds cladosporium herbarum, alternaria alternata, aspergillus niger, penicillium notatum, and rhizopus nigricans were performed in 96 workers from the same sawmills. average concentrations of airborne fungi were 1,696-7,316 cfu/m ... | 2012 | 22742790 |
| some chronic rhinosinusitis patients have elevated populations of fungi in their sinuses. | to measure the populations of 36 fungi in the homes and sinuses of chronic rhinosinusitis (crs) and non-crs patients. | 2012 | 22532289 |
| ecological and physiological studies on soil fungi at western region, libya. | sixty three species and 5 varieties belonging to 30 fungal genera were collected from 75 soil samples. cultivated (29 genera and 58 species + 5 var.), desert (22 and 35 + 2 var.) and saline soil (21 and 41 + 1 var.) fungi were recovered on glucose-, cellulose- and 50% sucrose-czapek's agar at 28℃. the most common genera were alternaria, aspergillus, emericella, fusarium, mycosphaerella, nectria and penicillium. the most prevalent species from the three types of soils on the three types of media ... | 2008 | 23997599 |
| aerobic microorganisms associated with alfalfa leafcutter bees (megachile rotundata). | characterization of microorganisms associated with alfalfa leaf-cutter bee (megachile rotundata) nectar, pollen, provisions, larval guts, and frass (excreta) in alberta demonstrated a varied aerobic microflora. yeasts were isolated frequently from nectar, pollen, and provisions but rarely from guts or frass. the most prevalent yeast taxa were: candida bombicola, cryptococcus albidus, metschnikowia reukaufii, and rhodotorula glutinis. although few filamentous fungi were found in nectar, they were ... | 1993 | 24190009 |
| recurrence of stachybotrys chartarum during mycological and toxicological study of bioaerosols collected in a dairy cattle shed. | agricultural occupations associated with animal breeding and the processing of animal materials in confinement systems could potentially lead to bioaerosol exposures. moulds and mycotoxins could be constituents of bioaerosols and should be studied because of their possible involvement in respiratory diseases and cancers. in order to characterize the fungal contamination of the indoor air in a dairy barn, bioaerosols were collected during 20 days in a cattle farm located in normandy (france). myc ... | 2012 | 22462447 |
| purification and characterization of a novel antifungal protein secreted by penicillium chrysogenum from an arctic sediment. | a fungal strain, penicillium chrysogenum a096, was isolated from an arctic sediment sample. its culture supernatant inhibited mycelial growth of some plant pathogenic fungi. after saturation of p. chrysogenum a096 culture supernatant with ammonium sulfate and ion exchange chromatography, a novel antifungal protein (pc-arctin) was purified and identified by matrix assisted laser desorption ionization-time of flight-time of flight-mass spectrometry (maldi-tof-tof-ms). the gene encoding for pc-arct ... | 2013 | 23474616 |
| control of household mycoflora in fermented sausages using phenolic fractions from olive mill wastewaters. | biopreservation using polyphenols represents an alternative to chemical molecules for improving food safety. in this work, we evaluated the antifungal activity of polyphenols extracted from olive mill wastewater (omwwp) to reduce or eliminate the growth of undesired fungi on the surface of dry fermented sausages. antagonism against penicillium expansum dsmz 1282, penicillium verrucosum dsmz 12639, penicillium nalgiovense ms01, aspergillus ochraceus dsmz 63304, cladosporium cladosporioides ms12, ... | 2015 | 25996624 |
| effect of storage conditions (relative humidity, duration, and temperature) on the germination time of aspergillus carbonarius and penicillium chrysogenum. | fungal conidia are disseminated, often in the air, for a certain period of time, prior to contaminating food products. the objective of this study was to examine the effects of the relative humidity, rh (%), time (day), and temperature (°c) during this period of time, called "storage", on the germination time, τ (h), of aspergillus carbonarius and penicillium chrysogenum. a doehlert design was used in the range, 20-100% rh, 2-28 days, and 5-25 °c. as compared to un-stored conidia, the germinatio ... | 2012 | 23141649 |
| genomic characteristics comparisons of 12 food-related filamentous fungi in trna gene set, codon usage and amino acid composition. | filamentous fungi are widely exploited in food industry due to their abilities to secrete large amounts of enzymes and metabolites. the recent availability of fungal genome sequences has provided an opportunity to explore the genomic characteristics of these food-related filamentous fungi. in this paper, we selected 12 representative filamentous fungi in the areas of food processing and safety, which were aspergillus clavatus, a. flavus, a. fumigatus, a. nidulans, a. niger, a. oryzae, a. terreus ... | 2012 | 22305983 |
| a penicillium chrysogenum-based expression system for the production of small, cysteine-rich antifungal proteins for structural and functional analyses. | small, cysteine-rich and cationic antifungal proteins (aps) from filamentous ascomycetes, such as nfap from neosartorya fischeri and paf from penicillium chrysogenum, are promising candidates for novel drug development. a prerequisite for their application is a detailed knowledge about their structure-function relation and mode of action, which would allow protein modelling to enhance their toxicity and specificity. technologies for structure analyses, such as electronic circular dichroism (ecd) ... | 2016 | 27835989 |
| insight into the antifungal mechanism of neosartorya fischeri antifungal protein. | small, cysteine-rich, highly stable antifungal proteins secreted by filamentous ascomycetes have great potential for the development of novel antifungal strategies. however, their practical application is still limited due to their not fully clarified mode of action. the aim of this work was to provide a deep insight into the antifungal mechanism of neosartorya fischeri antifungal protein (nfap), a novel representative of this protein group. within a short exposure time to nfap, reduced cellular ... | 2015 | 25994413 |
| sequencing of mitochondrial genomes of nine aspergillus and penicillium species identifies mobile introns and accessory genes as main sources of genome size variability. | the genera aspergillus and penicillium include some of the most beneficial as well as the most harmful fungal species such as the penicillin-producer penicillium chrysogenum and the human pathogen aspergillus fumigatus, respectively. their mitochondrial genomic sequences may hold vital clues into the mechanisms of their evolution, population genetics, and biology, yet only a handful of these genomes have been fully sequenced and annotated. | 2012 | 23234273 |
| biochemical characterization of a gh53 endo-β-1,4-galactanase and a gh35 exo-β-1,4-galactanase from penicillium chrysogenum. | an endo-β-1,4-galactanase (pcgal1) and an exo-β-1,4-galactanase (pcgalx35c) were purified from the culture filtrate of penicillium chrysogenum 31b. pcgal1 and pcgalx35c cdnas encoding pcgal1 and pcgalx35c were isolated by in vitro cloning. the deduced amino acid sequences of pcgal1 and pcgalx35c are highly similar to a putative endo-β-1,4-galactanase of aspergillus terreus (70% amino acid identity) and a putative β-galactosidase of neosartorya fischeri (72%), respectively. pfam analysis revealed ... | 2013 | 22584433 |
| antimicrobial potential of consolidation polymers loaded with biological copper nanoparticles. | biodeterioration of historic monuments and stone works by microorganisms takes place as a result of biofilm production and secretion of organic compounds that negatively affect on the stone matrix. | 2016 | 27400968 |
| lactobacillus plantarum with broad antifungal activity: a promising approach to increase safety and shelf-life of cereal-based products. | cereal-based fermented products are worldwide diffused staple food resources and cereal-based beverages represent a promising innovative field in the food market. contamination and development of spoilage filamentous fungi can result in loss of cereal-based food products and it is a critical safety concern due to their potential ability to produce mycotoxins. lactic acid bacteria (lab) have been proposed as green strategy for the control of the moulds in the food industry due to their ability to ... | 2017 | 27240933 |
| in vitro screening of mucus and solvent extracts of eisenia foetida against human bacterial and fungal pathogens. | earthworms are macro invertebrate and have been widely used as therapeutic drugs for thousands of years. in the current research, experiments viz., the antibacterial, antifungal and antioxidant activity of mucus and solvent extracts of eisenia foetida were conducted to investigate for the first time in pakistan against human infectious pathogens. antimicrobial activity of e. foetida against human pathogens underwent investigation through an agar disc diffusion method while an abts(•+) free radic ... | 2016 | 27166541 |
| role of nutrients and illuminance in predicting the fate of fungal mediated petroleum hydrocarbon degradation and biomass production. | biodegradation and biomass production are affected by numerous environmental factors including ph, oxygen availability and presence of pollutants. the present study, for the first time, elucidated the effects of nutrients and light on mycodegradation of petroleum hydrocarbons in diesel oil. seven fungal strains (aspergillus terreus fa3, aspergillus niger fa5, aspergillus terreus fa6, penicillium chrysogenum fp4, aspergillus terreus fp6, aspergillus flavus fp10, and candida sp. fg1) were used for ... | 2016 | 27039364 |
| occurrence of moulds from bee pollen in central italy--a preliminary study. | the present study aimed to preliminary evaluate the occurrence of fungi in 40 specimens of trapped pollen collected from april-september 2015 in 40 apiaries from tuscany (central italy). cultural and microscopical examinations allowed the recognition of cladosporium sp., alternaria sp., humicola sp. mucoraceae and acremonium sp. penicillium brevicompactum, penicillium chrysogenum, aspergillus flavus , aspergillus nidulans, aspergillus niger and aspergillus terreus were also identified. yeasts an ... | 2016 | 27010410 |
| evidence for synergistic activity of plant-derived essential oils against fungal pathogens of food. | the antifungal activities of eight essential oils (eos) namely basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree and thyme were evaluated for their ability to inhibit growth of aspergillus niger, aspergillus flavus, aspergillus parasiticus and penicillium chrysogenum. the antifungal activity of the eos was assessed by the minimum inhibitory concentration (mic) using 96-well microplate analysis. the interactions between different eo combinations were done by the checkerboard te ... | 2016 | 26678126 |
| lactic acid bacteria as functional probiotic isolates for inhibiting the growth of aspergillus flavus, a. parasiticus, a. niger and penicillium chrysogenum. | the aim of this study was to assess the potential of lactic acid bacteria (lab) such as lactobacillus acidophilus, l. rhamnosus, l. casei, l. paracasei and bifidobacterium bifidum to inhibit the outgrowth of some common food-spoiling fungi including aspergillus niger, a. flavus, a. parasiticus and penicillium chrysogenum. | 2015 | 26597145 |
| the effect of anatase and rutile crystallites isolated from titania p25 photocatalyst on growth of selected mould fungi. | antifungal properties of anatase and rutile crystallites isolated from commercial titania p25 photocatalyst were investigated by mycelium growth in the dark and under indoor light. investigated fungi, i.e., pseudallescheria boydii, scedosporium apiospermum, pseudallescheria ellipsoidea, scedosporium aurantiacum, aspergillus versicolor, aspergillus flavus, stachybotrys chartarum, penicillium chrysogenum, aspergillus melleus, were isolated from air and from moisture condensed on walls. anatase and ... | 2015 | 26177513 |
| impact of the antifungal protein pgafp from penicillium chrysogenum on the protein profile in aspergillus flavus. | antifungal proteins produced by molds are generally small, highly basic, and cysteine-rich. the best known effects of these proteins include morphological changes, metabolic inactivation, and membrane perturbation on sensitive fungi. reactive oxygen species (ros) generation leads to apoptosis, with g -protein playing a key role in transduction of cell death signals. the antifungal protein pgafp from penicillium chrysogenum inhibits growth of some toxigenic molds. here we analyzed the effect of t ... | 2015 | 26078108 |
| cytotoxic and antifungal activities of melleolide antibiotics follow dissimilar structure-activity relationships. | the fungal genus armillaria is unique in that it is the only natural source of melleolide antibiotics, i.e., protoilludene alcohols esterified with orsellinic acid or its derivatives. this class of natural products is known to exert antimicrobial and cytotoxic effects. here, we present a refined relationship between the structure and the antimicrobial activity of the melleolides. using both agar diffusion and broth dilution assays, we identified the δ(2,4)-double bond of the protoilludene moiety ... | 2014 | 24906293 |
| screening of indigenously isolated fungi for lovastatin production and its in vivo evaluation. | seven indigenously isolated fungal strains (aspergillus niger, aspergillus flavus, aspergillus terreus, penicillium citrinum, penicillium notatum, pleurotus ostreatus and trichoderma viradae) were tested for their potential to produce cholesterol lowering drug lovastatin by using different agro-industrial wastes (corn cobs, corn stover, banana stalk, wheat straw, wheat bran, bagasse) in submerged as well as solid state fermentation. aspergillus terreus showed maximum production of 18.74 mg/100 m ... | 2014 | 24894549 |
| microwave assisted synthesis of dihydrobenzo[4,5]imidazo[1,2-a]pyrimidin-4-ones; synthesis, in vitro antimicrobial and anticancer activities of novel coumarin substituted dihydrobenzo[4,5]imidazo[1,2-a]pyrimidin-4-ones. | the present article describes the synthesis of dihydrobenzo[4,5]imidazo[1,2-a]pyrimidin-4-one (2a-h) under microwave irradiation. the product was obtained in excellent yield (74-94%) in a shorter reaction time (2 min). these molecules (2a, b) further reacted with various substituted 4-bromomethylcoumarins (3a-f) to yield a new series of coumarin substituted dihydrobenzo[4,5]imidazo[1,2-a]pyrimidin-4-ones (4a-h). the structure of all the synthesized compounds were confirmed by spectral studies an ... | 2013 | 24056147 |
| a preliminary survey on the occurrence of mycotoxigenic fungi and mycotoxins contaminating red rice at consumer level in selangor, malaysia. | red rice is a fermented product of monascus spp. it is widely consumed by malaysian chinese who believe in its pharmacological properties. the traditional method of red rice preparation disregards safety regulation and renders red rice susceptible to fungal infestation and mycotoxin contamination. a preliminary study was undertaken aiming to determine the occurrence of mycotoxigenic fungi and mycotoxins contamination on red rice at consumer level in selangor, malaysia. fifty red rice samples wer ... | 2013 | 23242851 |
| mycobiota and mycotoxins (aflatoxins and ochratoxin) associated with some saudi date palm fruits. | this study aimed to determine the mycological profile of the retail date fruits distributed in different markets at taif, saudi arabia. the presence of aflatoxins and ochratoxin a was also measured. twenty-two fungal species belonging to 12 genera were isolated from 50 different date samples. aspergillus flavus, a. niger, penicillium chrysogenum, and rhizopus stolonifer were the most prevalent species among isolated fungi. eighty isolates of a. flavus and 36 of a. niger were detected for their a ... | 2012 | 22568750 |
| inhibitory effects of gossypol, gossypolone, and apogossypolone on a collection of economically important filamentous fungi. | racemic gossypol and its related derivatives gossypolone and apogossypolone demonstrated significant growth inhibition against a diverse collection of filamentous fungi that included aspergillus flavus, aspergillus parasiticus, aspergillus alliaceus, aspergillus fumigatus, fusarium graminearum, fusarium moniliforme, penicillium chrysogenum, penicillium corylophilum, and stachybotrys atra. the compounds were tested in a czapek agar medium at a concentration of 100 μg/ml. racemic gossypol and apog ... | 2012 | 22324794 |
| effect of some food preservatives on the lipolytic activity of beef luncheon fungi. | beef luncheon meat is one of the most popular meals in several countries in the world including egypt. thirty one fungal species and 3 species varieties were recovered from 30 samples of beef luncheon meat collected from different supermarkets in qena. alternaria, aspergillus, emericella, mucor, mycosphaerella, penicillium and rhizopus were the most common genera on the two types of media. from the above genera, the most prevalent species were alternaria alternate, aspergillus flavus, a. fumigat ... | 2008 | 23997619 |
| application of a low molecular weight antifungal protein from penicillium chrysogenum (paf) to treat pulmonary aspergillosis in mice. | paf, a small antifungal protein from penicillium chrysogenum, inhibits the growth of several pathogenic filamentous fungi, including members of the aspergillus genus. paf has been proven to have no toxic effects in vivo in mice by intranasal application. to test its efficacy against invasive pulmonary aspergillosis (ipa), experiments were carried out in mice suffering from ipa. adult mice were immunosuppressed and then infected with aspergillus fumigatus. after stable infection, the animals were ... | 2016 | 27826143 |
| the relationship between biomarkers of fungal allergy and lung damage in asthma. | immunological biomarkers are the key to the diagnosis of allergic bronchopulmonary aspergillosis (abpa) and fungal sensitisation, but how these relate to clinically relevant outcomes is unclear. | 2017 | 27805757 |
| efficacy of aqueous and methanolic extracts of rheum spiciformis against pathogenic bacterial and fungal strains. | rheum spiciformis is a newly identified edible medicinal plant of genus rheum. the plant is used to treat various diseases on traditional levels in kashmir valley, india. | 2016 | 27790424 |
| biodegradative activities of selected environmental fungi on a polyester polyurethane varnish and polyether polyurethane foams. | polyurethane (pu) is widely used in many aspects of modern life because of its versatility and resistance. however, pu waste disposal generates large problems, since it is slowly degraded, there are limited recycling processes, and its destruction may generate toxic compounds. in this work, we isolated fungal strains able to grow in mineral medium with a polyester pu (ps-pu; impranil dln) or a polyether pu (pe-pu; poly lack) varnish as the only carbon source. of the eight best impranil-degrading ... | 2016 | 27316963 |
| profile and morphology of fungal aerosols characterized by field emission scanning electron microscopy (fesem). | fungal aerosols consist of spores and fragments with diverse array of morphologies; however, the size, shape, and origin of the constituents require further characterization. in this study, we characterize the profile of aerosols generated from aspergillus fumigatus, a. versicolor, and penicillium chrysogenum grown for 8 weeks on gypsum boards. fungal particles were aerosolized at 12 and 20 l min(-1) using the fungal spore source strength tester (fssst) and the stami particle generator (spg). co ... | 2017 | 26855468 |
| the mycobiota of air inside and outside the meju fermentation room and the origin of meju fungi. | the fungi on meju are known to play an important role as degrader of macromolecule of soybeans. in order to elucidate the origin of fungi on traditional meju, mycobiota of the air both inside and outside traditional meju fermentation rooms was examined. from 11 samples of air collected from inside and outside of 7 meju fermentation rooms, 37 genera and 90 species of fungi were identified. in outside air of the fermentation room, cladosporium sp. and cladosporium cladosporioides were the dominant ... | 2015 | 26539041 |
| probiotic potential of lactobacillus strains with antifungal activity isolated from animal manure. | the aim of the study was to isolate and characterize the lactic acid bacteria (lab) from animal manure. among the thirty lab strains, four strains, namely, kcc-25, kcc-26, kcc-27, and kcc-28, showed good cell growth and antifungal activity and were selected for further characterization. biochemical and physiology properties of strains confirmed that the strains are related to the lactobacillus sp.; further, the 16s rrna sequencing confirmed 99.99% sequence similarity towards lactobacillus planta ... | 2015 | 26167534 |
| an evaluation of antifungal agents for the treatment of fungal contamination in indoor air environments. | fungal contamination in indoor environments has been associated with adverse health effects for the inhabitants. remediation of fungal contamination requires removal of the fungi present and modifying the indoor environment to become less favourable to growth. this may include treatment of indoor environments with an antifungal agent to prevent future growth. however there are limited published data or advice on chemical agents suitable for indoor fungal remediation. the aim of this study was t ... | 2015 | 26042369 |
| in-vitro assessment of the probiotic potential of lactobacillus plantarum kcc-24 isolated from italian rye-grass (lolium multiflorum) forage. | the aim of the present study was to determine the probiotic potential of the lactic acid bacteria lactobacillus plantarum kcc-24 (l. plantarum kcc-24), that was isolated and characterized from italian ryegrass (lolium multiflorum) forage. the following experiments were performed to assess the probiotic characteristics such as antifungal activity, antibiotic susceptibility, resistance to low ph, stimulated gastric juice and bile salts, proteolytic activity, auto-aggregation, cell surface hydropho ... | 2015 | 25576238 |
| submicronic fungal bioaerosols: high-resolution microscopic characterization and quantification. | submicronic particles released from fungal cultures have been suggested to be additional sources of personal exposure in mold-contaminated buildings. in vitro generation of these particles has been studied with particle counters, eventually supplemented by autofluorescence, that recognize fragments by size and discriminate biotic from abiotic particles. however, the fungal origin of submicronic particles remains unclear. in this study, submicronic fungal particles derived from aspergillus fumiga ... | 2014 | 25217010 |
| concentration of bioaerosols in composting plants using different quantification methods. | bioaerosols (organic dusts) containing viable and non-viable microorganisms and their metabolic products can lead to adverse health effects in exposed workers. standard quantification methods of airborne microorganisms are mainly based on cultivation, which often underestimates the microbial burden. the aim of the study was to determine the microbial load in german composting plants with different, mainly cultivation-independent, methods. second purpose was to evaluate which working areas are as ... | 2014 | 24759376 |
| exposure assessment of lovastatin in pu-erh tea. | this paper reports the results of an extensive survey on the levels of lovastatin in pu-erh tea samples. the microbial source of lovastatin was assessed by testing the ability of fungi with higher isolation frequency in the pu-erh tea samples to produce lovastatin on czapek yeast extract agar (cya). lovastatin was not detected in any of the raw pu-erh tea samples without storage but was found in almost all the ripe pu-erh tea samples, with lovastatin contents ranging from 20.61 ng/gdw to 226.38 ... | 2013 | 23587710 |
| volatile composition and antimicrobial activity of the essential oil of artemisia absinthium growing in western ghats region of north west karnataka, india. | artemisia absinthium l. (asteraceae) is an aromatic, herbaceous, perennial plant commonly known as wormwood. artemisia absinthium is traditionally used as an anthelmintic, antiseptic, antispasmodic and for bacillary dysentery, cancers and neurodegenerative diseases. | 2013 | 23570523 |
| broad-spectrum antifungal-producing lactic acid bacteria and their application in fruit models. | a large-scale screen of some 7,000 presumptive lactic acid bacteria (lab), isolated from animal, human, or plant origin, identified 1,149 isolates with inhibitory activity against the food-spoilage mould penicillium expansum. in excess of 500 lab isolates were subsequently identified to produce a broad spectrum of activity against p. expansum, penicillium digitatum, penicillium notatum, penicillium roqueforti, rhizopus stolonifer, fusarium culmorum, aspergillus fumigatus and rhodotorula mucilagi ... | 2013 | 23160868 |
| development and application of mold antigen-specific enzyme-linked immunosorbent assays (elisa) to quantify airborne antigen exposure. | the aim of our study was to develop specific enzyme-linked immunosorbent assays (elisas) and apply these to assess mold antigen exposure in composting plants. sandwich elisas based on polyclonal antibodies to aspergillus fumigatus (af), penicillium chrysogenum (pc), and cladosporium herbarum (ch) antigens were developed and validated. reactivity to 18 different mold species was tested. to optimize extraction procedure, inhalable dust samples taken by a parallel sampler were extracted with or wit ... | 2012 | 22994572 |
| identification of ochratoxin a producing fungi associated with fresh and dry liquorice. | the presence of fungi on liquorice could contaminate the crop and result in elevated levels of mycotoxin. in this study, the mycobiota associated with fresh and dry liquorice was investigated in 3 producing regions of china. potential toxigenic fungi were tested for ochratoxin a (ota) and aflatoxin b1 (afb1) production using liquid chromatography/mass spectrometry/mass spectrometry. based on a polyphasic approach using morphological characters, β-tubulin and rna polymerase ii second largest subu ... | 2013 | 24205182 |
| d-galactose catabolism in penicillium chrysogenum: expression analysis of the structural genes of the leloir pathway. | in this study, we analyzed the expression of the structural genes encoding the five enzymes comprising the leloir pathway of d-galactose catabolism in the industrial cell factory penicillium chrysogenum on various carbon sources. the genome of p. chrysogenum contains a putative galactokinase gene at the annotated locus pc13g10140, the product of which shows strong structural similarity to yeast galactokinase that was expressed on lactose and d-galactose only. the expression profile of the galact ... | 2016 | 27630054 |
| key role of laea and velvet complex proteins on expression of β-lactam and pr-toxin genes in penicillium chrysogenum: cross-talk regulation of secondary metabolite pathways. | penicillium chrysogenum is an excellent model fungus to study the molecular mechanisms of control of expression of secondary metabolite genes. a key global regulator of the biosynthesis of secondary metabolites is the laea protein that interacts with other components of the velvet complex (vela, velb, velc, vosa). these components interact with laea and regulate expression of penicillin and pr-toxin biosynthetic genes in p. chrysogenum. both laea and vela are positive regulators of the penicilli ... | 2016 | 27565675 |
| generation and characterization of indoor fungal aerosols for inhalation studies. | in the indoor environment, people are exposed to several fungal species. evident dampness is associated with increased respiratory symptoms. to examine the immune responses associated with fungal exposure, mice are often exposed to a single species grown on an agar medium. the aim of this study was to develop an inhalation exposure system to be able to examine responses in mice exposed to mixed fungal species aerosolized from fungus-infested building materials. indoor airborne fungi were sampled ... | 2016 | 26921421 |
| penicillium antifungal protein (paf) is involved in the apoptotic and autophagic processes of the producer penicillium chrysogenum. | paf, which is produced by the filamentous fungus pencicillium chrysogenum, is a small antifungal protein, triggering ros-mediated apoptotic cell death in aspergillus nidulans. in this work, we provide information on the function of paf in the host p. chrysogenum considering that carbon-starving cultures of the δpaf mutant strain showed significantly reduced apoptosis rates in comparison to the wild-type (wt) strain. moreover, the addition of paf to the δpaf strain resulted in a twofold increase ... | 2014 | 25261948 |
| direct involvement of the crea transcription factor in penicillin biosynthesis and expression of the pcbab gene in penicillium chrysogenum. | the transcription factor crea is the main regulator responsible for carbon repression in filamentous fungi. crea is a wide domain regulator that binds to regulatory elements in the promoters of target genes to repress their transcription. penicillin biosynthesis and the expression of penicillin biosynthetic genes are subject to carbon repression. however, evidence of the participation of crea in this regulation is still lacking, and previous studies on the promoter of the pcbc gene of aspergillu ... | 2014 | 24818689 |
| extra- and intracellular lactose catabolism in penicillium chrysogenum: phylogenetic and expression analysis of the putative permease and hydrolase genes. | penicillium chrysogenum is used as an industrial producer of penicillin. we investigated its catabolism of lactose, an abundant component of whey used in penicillin fermentation, comparing the type strain nrrl 1951 with the high producing strain as-p-78. both strains grew similarly on lactose as the sole carbon source under batch conditions, exhibiting almost identical time profiles of sugar depletion. in silico analysis of the genome sequences revealed that p. chrysogenum features at least five ... | 2014 | 24690910 |
| morphological changes induced by class iii chitin synthase gene silencing could enhance penicillin production of penicillium chrysogenum. | chitin synthases catalyze the formation of β-(1,4)-glycosidic bonds between n-acetylglucosamine residues to form the unbranched polysaccharide chitin, which is the major component of cell walls in most filamentous fungi. several studies have shown that chitin synthases are structurally and functionally divergent and play crucial roles in the growth and morphogenesis of the genus aspergillus although little research on this topic has been done in penicillium chrysogenum. we used blast to find the ... | 2013 | 23179625 |
| expression of fungal genes involved in penicllin biosynthesis. | carbon catabolite repression and ph regulation are regulatory circuits with a wide domain of action in the plectomycetes. penicillin biosynthesis is one of the pathways which are under their control. the conclusions obtained so far, which are based on studies of the genetic and molecular regulation of the penicillin pathway of aspergillus nidulans, would have been much harder to produce using an organism such as penicillium chrysogenum (the industrial penicillin producer). however, a. nidulans a ... | 1993 | 24420113 |
| evaluating the effects of microwave-assisted hydrodistillation on antifungal and radical scavenging activities of oliveria decumbens and chaerophyllum macropodum essential oils. | in this study, radical scavenging and antifungal activities of chaerophyllum macropodum and oliveria decumbens essential oils (eos) extracted with microwave-assisted hydrodistillation (mahd) were evaluated in comparison with the same eos extracted by conventional hydrodistillation (hd). the final eo yields that were obtained using hd (after 150 min) and mahd (after 45 min) were 1.72 and 1.67% for c. macropodum and 8.10 and 7.91% for o. decumbens, respectively. there were no significant differenc ... | 2017 | 28371590 |
| effect of magnesium acetate on the antimold activity of lactobacillus. | the antimold activity of lactic acid bacteria (lab) is used in food biopreservation. the aim of this study was to evaluate the effect of magnesium acetate added to de man rogosa sharpe (mrs) medium on the antimold activity of three lab strains ( lactobacillus plantarum , lactobacillus brevis , and lactobacillus fermentum ) against molds contaminating food ( aspergillus oryzae , aspergillus niger , penicillium chrysogenum , fusarium avenaceum , and rhizopus arrhizus ) and their ability to produce ... | 2017 | 28221876 |
| can fungi compete with marine sources for chitosan production? | chitosan, a β-1,4-linked glucosamine polymer is formed by deacetylation of chitin. it has a wide range of applications from agriculture to human health care products. chitosan is commercially produced from shellfish, shrimp waste, crab and lobster processing using strong alkalis at high temperatures for long time periods. the production of chitin and chitosan from fungal sources has gained increased attention in recent years due to potential advantages in terms of homogenous polymer length, high ... | 2017 | 28143744 |
| synthesis, structure and antimicrobial properties of novel benzalkonium chloride analogues with pyridine rings. | quaternary ammonium compounds (qacs) are a group of compounds of great economic significance. they are widely used as emulsifiers, detergents, solubilizers and corrosion inhibitors in household and industrial products. due to their excellent antimicrobial activity qacs have also gained a special meaning as antimicrobials in hospitals, agriculture and the food industry. the main representatives of the microbiocidal qacs are the benzalkonium chlorides (bacs), which exhibit biocidal activity agains ... | 2017 | 28098790 |
| silver nanoparticles: a mechanism of action on moulds. | silver nanoparticles (agnps) are widely used in all branches of industry. however, their mechanisms of action towards moulds have not been studied yet. thus we conducted this study in which we have used laser desorption/ionization time-of-flight mass spectrometry (ldi-tof-ms) analysis to determine metabolomic changes, and microscopic analysis (transmission electron microscopy, fluorescent microscopy) to observe changes in mould cells. the agnp treatment caused the downregulation of 162 (15 ppm) ... | 2016 | 27896349 |
| antifungal, anticancer and aminopeptidase inhibitory potential of a phenazine compound produced by lactococcus bsn307. | a bioactive compound was purified from the culture medium of a new strain of lactococcus bsn307 by solvent extraction followed by chromatographic techniques. this bioactive compound was identified to belong to phenazine class of compounds by ms, nmr and ftir. the phenazine compound showed antifungal activity against aspergillus niger, penicillium chrysogenum as well as fusarium oxysporum by disc diffusion assay in addition to antioxidant potential as demonstrated by dpph scavenging assay. the co ... | 2016 | 27784936 |
| winery biomass waste degradation by sequential sonication and mixed fungal enzyme treatments. | to increase the efficiency of winery-derived biomass biodegradation, grape pomace was ultrasonicated for 20min in the presence of 0.25m, 0.5mand1.0mkoh and 1.0mnaoh. this was followed by treatment with a 1:1 (v/v) mix of crude enzyme preparation derived from phanerochaete chrysosporium and trametes versicolor for 18h and a further 18h treatment with a 60:14:4:2 percent ratio combination of enzymes derived from aspergillus niger: penicillium chrysogenum: trichoderma harzianum: p. citrinum, repsec ... | 2016 | 27599392 |
| shedding light on aspergillus niger volatile exometabolome. | an in-depth exploration of the headspace content of aspergillus niger cultures was performed upon different growth conditions, using a methodology based on advanced multidimensional gas chromatography. this volatile fraction comprises 428 putatively identified compounds distributed over several chemical families, being the major ones hydrocarbons, alcohols, esters, ketones and aldehydes. these metabolites may be related with different metabolic pathways, such as amino acid metabolism, biosynthes ... | 2016 | 27264696 |
| transcriptomic responses of mixed cultures of ascomycete fungi to lignocellulose using dual rna-seq reveal inter-species antagonism and limited beneficial effects on cazyme expression. | gaining new knowledge through fungal monoculture responses to lignocellulose is a widely used approach that can lead to better cocktails for lignocellulose saccharification (the enzymatic release of sugars which are subsequently used to make biofuels). however, responses in lignocellulose mixed cultures are rarely studied in the same detail even though in nature fungi often degrade lignocellulose as mixed communities. using a dual rna-seq approach, we describe the first study of the transcriptio ... | 2016 | 27150814 |
| antimicrobial potentials of different solvent extracted samples from physalis ixocarpa. | the present study investigates the antimicrobial activities of different solvent extracted samples isolated from different parts of physalis ixocarpa through disc diffusion assay using three different concentrations. statistical analysis of the data revealed that different parts of the plant showed varying degree of inhibition against different bacteria at different concentrations. different solvent extracted samples from the calyx showed inhibitory activity against most of the bacteria under st ... | 2016 | 27087074 |
| antimicrobial properties of black grape (vitis vinifera l.) peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds. | black grape peel possesses a substantial amount of polyphenolic antimicrobial compounds that can be used for controlling the growth of pathogenic microorganisms. the purpose of this study was to assess antibacterial and antifungal activity of black grape peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds, respectively. | 2017 | 26729960 |
| new promoters for strain engineering of penicillium chrysogenum. | filamentous fungi such as aspergillus and penicillium are widely used as hosts for the industrial products such as proteins and secondary metabolites. although filamentous fungi are versatile in recognizing transcriptional and translational elements present in genes from other filamentous fungal species, only few promoters have been applied and compared in performance so far in penicillium chrysogenum. therefore, a set of homologous and heterologous promoters were tested in a reporter system to ... | 2016 | 26701309 |
| microwave assisted one-pot catalyst free green synthesis of new methyl-7-amino-4-oxo-5-phenyl-2-thioxo-2,3,4,5-tetrahydro-1h-pyrano[2,3-d]pyrimidine-6-carboxylates as potent in vitro antibacterial and antifungal activity. | an efficiently simple protocol for the synthesis of methyl 7 amino-4-oxo-5-phenyl-2-thioxo-2, 3, 4,5-tetrahydro-1h-pyrano[2,3-d]pyrimidine-6-carboxylates via one-pot three component condensation pathway is established via microwave irradiation using varied benzaldehyde derivatives, methylcyanoacetate and thio-barbituric acid in water as a green solvent. a variety of functionalized substrates were found to react under this methodology due to its easy operability and offers several advantages like ... | 2015 | 26644932 |
| proteomic analysis of anti-cancerous scopularide production by a marine microascus brevicaulis strain and its uv mutant. | the marine fungus microascus brevicaulis strain lf580 is a non-model secondary metabolite producer with high yields of the two secondary metabolites scopularides a and b, which exhibit distinct activities against tumour cell lines. a mutant strain was obtained using uv mutagenesis, showing faster growth and differences in pellet formation besides higher production levels. here, we show the first proteome study of a marine fungus. comparative proteomics were applied to gain deeper understanding o ... | 2015 | 26460745 |