cassava interspecific hybrids with increased protein content and improved amino acid profiles. | cassava (manihot esculenta) is a principal food for large populations of poor people in the tropics and subtropics. its edible roots are poor in protein and lack several essential amino acids. interspecific hybrids may acquire high protein characteristics from wild species. we analyzed 19 hybrids of m. esculenta with its wild relative, m. oligantha, for crude protein, amino acid profile, and total cyanide. some hybrids produced roots with high protein content of up to 5.7%, while the common cult ... | 2013 | 23661446 |
quantitative trait loci and candidate genes associated with starch pasting viscosity characteristics in cassava (manihot esculenta crantz). | starch pasting viscosity is an important quality trait in cassava (manihot esculenta crantz) cultivars. the aim here was to identify loci and candidate genes associated with the starch pasting viscosity. quantitative trait loci (qtl) mapping for seven pasting viscosity parameters was carried out using 100 lines of an f1 mapping population from a cross between two cassava cultivars huay bong 60 and hanatee. starch samples were obtained from roots of cassava grown in 2008 and 2009 at rayong, and i ... | 2014 | 23614826 |
visualizing metabolite distribution and enzymatic conversion in plant tissues by desorption electrospray ionization mass spectrometry imaging. | in comparison with the technology platforms developed to localize transcripts and proteins, imaging tools for visualization of metabolite distributions in plant tissues are less well developed and lack versatility. this hampers our understanding of plant metabolism and dynamics. in this study, we demonstrate that desorption electrospray ionization mass spectrometry imaging (desi-msi) of tissue imprints on porous teflon may be used to accurately image the distribution of even labile plant metabol ... | 2013 | 23551340 |
geographic differences in patterns of genetic differentiation among bitter and sweet manioc (manihot esculenta subsp. esculenta; euphorbiaceae). | | 2013 | 23548671 |
synthesis of periclinal chimera in cassava. | we provide the first report on the synthesis of a very productive interspecific periclinal chimera of cassava, with large and edible roots. the epidermal tissue of the chimera was formed by the cultivated species manihot esculenta (e), and the subepidermis and internal tissue were formed by the wild species, manihot fortalezensis (f). we used cytogenetics and morphological analyses to determine the origins of all tissues. these results may offer potential for the development of new lines for cro ... | 2013 | 23512678 |
relative contribution of biotic and abiotic factors to the population density of the cassava green mite, mononychellus tanajoa (acari: tetranychidae). | the cassava green mite, mononychellus tanajoa, is a key pest of cassava, manihot esculenta crantz (euphorbiaceae), and it may be kept in check by naturally occurring predatory mites of the family phytoseiidae. in addition to predatory mites, abiotic factors may also contribute to regulate pest mite populations in the field. here, we evaluated the population densities of both m. tanajoa and the generalist predatory mite euseius ho deleon (acari: phytoseiidae) over the cultivation cycle (11 months ... | 2013 | 23417702 |
molecular and supra-molecular structure of waxy starches developed from cassava (manihot esculenta crantz). | the aim of this work was to characterize the amylopectin of low amylose content cassava starches obtained from transgenesis comparatively with a natural waxy cassava starch (wxn) discovered recently in ciat (international center for tropical agriculture). macromolecular features, starch granule morphology, crystallinity and thermal properties of these starches were determined. m¯(w) of amylopectin from the transgenic varieties are lower than wxn. branched and debranched chain distributions analy ... | 2013 | 23399176 |
development of model for barrier and optical properties of tapioca starch based edible films. | the film forming solutions composed of tapioca (cassava) starch (1-3 g), glycerol (0.5-1.0 ml), agar (0.5-1.0 g) and span 80 (0.1-0.5 ml) were prepared according to a three-level, four-factor box-behnken response surface experimental design. the films were obtained by casting method and they are homogenous and transparent. the influence of film composition (tapioca starch, glycerol, agar and span80) on the barrier and optical properties of the tapioca starch based edible films was evaluated. the ... | 2013 | 23399163 |
comparative morphology, biology and histology of reproductive development in three lines of manihot esculenta crantz (euphorbiaceae: crotonoideae). | cassava (manihot esculenta), a major food staple in the tropics and subtropics, thrives even in environments undergoing threatening climate change. to satisfy the increasing demand for crop improvement and overcome the limitations of conventional breeding, the introduction of inbreeding techniques such as the production of doubled haploid lines via androgenesis or gynogenesis offers advantages. however, comprehensive studies on cassava flower bud biology or structural development are lacking and ... | 2013 | 23346343 |
enhanced reactive oxygen species scavenging by overproduction of superoxide dismutase and catalase delays postharvest physiological deterioration of cassava storage roots. | postharvest physiological deterioration (ppd) of cassava (manihot esculenta) storage roots is the result of a rapid oxidative burst, which leads to discoloration of the vascular tissues due to the oxidation of phenolic compounds. in this study, coexpression of the reactive oxygen species (ros)-scavenging enzymes copper/zinc superoxide dismutase (mecu/znsod) and catalase (mecat1) in transgenic cassava was used to explore the intrinsic relationship between ros scavenging and ppd occurrence. transg ... | 2013 | 23344905 |
effects of dietary fibers with different fermentation characteristics on feeding motivation in adult female pigs. | dietary fibers can be fermented in the colon, resulting in production of short-chain fatty acids (scfa) and secretion of satiety-related peptides. fermentation characteristics (fermentation kinetics and scfa-profile) differ between fibers and could impact their satiating potential. we investigated the effects of fibers with varying fermentation characteristics on feeding motivation in adult female pigs. sixteen pair-housed pigs received four diets in four periods in a latin square design. starch ... | 2013 | 23313406 |
effects of starches on the textural, rheological, and color properties of surimi-beef gels with microbial tranglutaminase. | in order to evaluate effects of starches (corn starch, potato starch, and tapioca starch) on the characteristics of surimi-beef gels with microbial transglutaminase, the cooking loss, gel strength, color and rheological properties of samples were investigated. results demonstrated that starches gave negative effects on the cooking loss of surimi-beef gels. the gel with corn starch had the highest cooking loss while that with tapioca starch showed the lowest value. the gel with potato starch obta ... | 2013 | 23273461 |
[x-ray diffraction study of high hydrostatic pressure on crystalline structure of different type starches]. | crystalline changes of different type starches after high hydrostatic pressure treated under 300, 450, 600 mpa were studied by x-ray diffraction. waxy maize (a type, 100% amylopectin), hylon vii (b type, 30% amylopectin) and tapioca starch (c type, 83% amylopectin) were chosen. the results indicated that for waxy maize starch, annealing effect was observed at 300 mpa, disappearance of crystalline structure happened at 450 mpa and retrogradation at 600 mpa. the results proved that the granule und ... | 2012 | 23240443 |
characterization of carotenoid-protein complexes and gene expression analysis associated with carotenoid sequestration in pigmented cassava (manihot esculenta crantz) storage root. | carotenoid-protein complex (cpc) was isolated from chromoplast-enriched suspensions of cassava storage root (csr) using size exclusion chromatography and characterized. peptide sequences (lc_ms/ms spectrum) obtained from cpc and their corresponding proteins were obtained using publically available databases. small heat shock proteins (shsps) were the most abundant proteins identified in the cpc. western blot analysis showed that fribrillin and or-protein were present in chromoplast-enriched susp ... | 2012 | 23230451 |
effects of lipids on enzymatic hydrolysis and physical properties of starch. | this study aimed to understand effects of lipids, including corn oil (co), soy lecithin (sl), palmitic acid (pa), stearic acid (sa), oleic acid (oa), and linoleic acid (la), on the enzymatic hydrolysis and physical properties of normal corn (ncs), tapioca (tps), waxy corn (wcs), and high-amylose corn (ha7) starch, and to elucidate mechanisms of interactions between the starches and lipids. after cooking with the lipids (10%, w/w, dsb), ncs, tps, and ha7 showed significant decreases in enzymatic ... | 2013 | 23218274 |
effect of resistant starch on the cooking quality of yam (dioscorea spp.) and cassava (manihot esculenta) based paste products. | total starch (ts) and resistant starch (rs) contents in pasty edible product of mealy and hard cooking tubers of three yam varieties and four cassava varieties were determined to evaluate their contribution in their cooking quality. ts and rs contents appeared as the main components in determining yam cooking quality. mealy cooking yam varieties were characterized by a significant higher ts content (75.2 ± 7.7 g/100 g d.m.) and lower rs content (13.8 ± 3.4 g/100 g d.m.) than hard cooking yam var ... | 2013 | 23215529 |
effect of harvesting frequency, variety and leaf maturity on nutrient composition, hydrogen cyanide content and cassava foliage yield. | the experiment studied the effect of harvesting frequencies and varieties on yield, chemical composition and hydrogen cyanide content in cassava foliage. foliage from three cassava varieties, k94 (very bitter), k98-7 (medium bitter) and a local (sweet), were harvested in three different cutting cycles, at 3, 6 and 9 months; 6 and 9 months and 9 months after planting, in a 2-yr experiment carried out in hanoi, vietnam. increasing the harvesting frequency increased dry matter (dm) and crude protei ... | 2012 | 25049534 |
cassava starch as a stabilizer of soy-based beverages. | soy-based beverages are presented as healthy food alternatives for human nutrition. cassava (manihot esculenta, crantz) starch is relatively inexpensive, widely available in brazil and is broadly used by the food industry due to its desired properties that result from pasting. the objective of this study was to develop soy-based beverages with good sensory quality using native cassava starch as a stabilizer and maintaining the nutritional value that makes this product a functional food. the deve ... | 2012 | 23144242 |
cloning and characterization of a tuberous root-specific promoter from cassava (manihot esculenta crantz). | in order to obtain a tuberous root-specific promoter to be used in the transformation of cassava, a 1,728 bp sequence containing the cassava granule-bound starch synthase (gbssi) promoter was isolated. the sequence proved to contain light- and sugar-responsive cis elements. part of this sequence (1,167 bp) was cloned into binary vectors to drive expression of the firefly luciferase gene. cassava cultivar adira 4 was transformed with this construct or a control construct in which the luciferase g ... | 2012 | 23132522 |
hev b 5: latex allergen implicated in clinically relevant cross-reactivity with manioc. | | 2012 | 23101196 |
mercury in the surface soil and cassava, manihot esculenta (flesh, leaves and peel) near goldmines at bogoso and prestea, ghana. | mercury amalgamation is used indiscriminately in the recovery of gold by small-scale native gem winners in ghana. mercury is released into the environment in the form of wastewater, tailing and vapor from the roasting of amalgam to separate gold. the study looked at the levels of total mercury concentration in surface soil and cassava crop from farms located within the vicinities of bogoso and prestea goldmines. the surface soil total mercury concentrations ranged between 125.29 and 352.52 μg/kg ... | 2012 | 23052587 |
starch addition in renneted milk gels: partitioning between curd and whey and effect on curd syneresis and gel microstructure. | milk gels were made by renneting and acidifying skim milk containing 5 different starches, and then compressed by centrifugation to express whey and simulate curd syneresis during the manufacture of low-fat cheese. a series of 17 starches were examined, with 5 starches being selected for in-depth analysis: a modified waxy corn starch (wc), a waxy rice starch (wr), an instant tapioca starch (it), a modified tapioca starch (mt), and dextrin (dx). milks containing wc, wr, and dx were given a 72°c h ... | 2012 | 23040026 |
iron biofortification and homeostasis in transgenic cassava roots expressing the algal iron assimilatory gene, fea1. | we have engineered the tropical root crop cassava (manihot esculenta) to express the chlamydomonas reinhardtii iron assimilatory gene, fea1, in its storage roots with the objective of enhancing the root nutritional qualities. iron levels in mature cassava storage roots were increased from 10 to 36 ppm in the highest iron accumulating transgenic lines. these iron levels are sufficient to meet the minimum daily requirement for iron in a 500 g meal. significantly, the expression of the fea1 gene in ... | 2012 | 22993514 |
first report of infestation of cassava fruit, manihot esculenta, by neosilba perezi (romero & ruppell) (lonchaeidae) in brazil. | | 2012 | 22990837 |
retraction: transgenic biofortification of the starchy staple cassava (manihot esculenta) generates a novel sink for protein. | | 2012 | 22970110 |
convergent adaptations: bitter manioc cultivation systems in fertile anthropogenic dark earths and floodplain soils in central amazonia. | shifting cultivation in the humid tropics is incredibly diverse, yet research tends to focus on one type: long-fallow shifting cultivation. while it is a typical adaptation to the highly-weathered nutrient-poor soils of the amazonian terra firme, fertile environments in the region offer opportunities for agricultural intensification. we hypothesized that amazonian people have developed divergent bitter manioc cultivation systems as adaptations to the properties of different soils. we compared bi ... | 2012 | 22952727 |
preparation and characterization of gelatinized granular starches from aqueous ethanol treatments. | in order to modify the properties of native starch granules, the formation of gelatinized granular forms (ggs) from normal, waxy, and high amylose maize, as well as potato and tapioca starches was investigated by treating granules with aqueous ethanol at varying starch:water:ethanol ratios and then heating in a rotary evaporator to remove ethanol. the modified starches were characterized using bright field, polarized and electron microscopy. short/long range molecular order and enthalpic transit ... | 2012 | 22944420 |
occurrence of lace bug vatiga illudens and vatiga manihotae (hemiptera: tingidae) in mato grosso do sul, midwestern brazil. | nymphs and adults of the lace bug (hemiptera: tingidae) have been found in cassava crops (manihot esculenta) in mato grosso do sul state, brazil. the insects were collected in the field and taken to the laboratory where they were identified based on some morphological traits of the species vatiga manihotae (drake) and v. illudens (drake), which are first reported in the aforementioned state. | 2012 | 22886161 |
research on motor neuron diseases konzo and neurolathyrism: trends from 1990 to 2010. | konzo (caused by consumption of improperly processed cassava, manihot esculenta) and neurolathyrism (caused by prolonged overconsumption of grass pea, lathyrus sativus) are two distinct non-infectious upper motor neurone diseases with identical clinical symptoms of spastic paraparesis of the legs. they affect many thousands of people among the poor in the remote rural areas in the central and southern parts of africa afflicting them with konzo in ethiopia and in the indian sub-continent with neu ... | 2012 | 22860149 |
effects of pulsed electric field treatments on some properties of tapioca starch. | tapioca starch-water dispersions (8.0%, w/w) were subject to pulsed electric fields (pef) at 30, 40 and 50 kv cm(-1), respectively. the effect of the intense energy input (q) of pef treatment (over 28.85 j g(-1)) on the functionality of tapioca starch was investigated. scanning electron microscopy analysis revealed the dissociation and damage of pef treated tapioca starch granules. x-ray diffraction analysis demonstrated that tapioca starch tended to transform from crystalline to amorphous struc ... | 2012 | 24750906 |
formamide-based rna isolation at above zero temperatures from high starch cassava tubers. | cassava (manihot esculenta) tubers are a main source of carbohydrate for a large percentage of people in the tropics. however, obtaining rna from such high starch tubers proves problematic because gelation occurs during standard isolation procedures. in preliminary studies, formamide was used to homogenise tubers at room temperature and no gelation was observed. | 2012 | 22826189 |
preparation of hybrid hydrogel containing ag nanoparticles by a green in situ reduction method. | in this article, large and uniform ag nanoparticle-containing hybrid hydrogels were prepared by in situ reduction of ag ions in cross-linked tapioca dialdehyde starch (das)-chitosan hydrogels. in the hybrid hydrogels, chitosan was chosen as a macromolecular cross-linker because of its abundant source and good biocompatibility. the hybrid hydrogel showed good water-swelling properties, which could be controlled by varying the ratio of chitosan to tapioca das in the hydrogel. the reductive aldehyd ... | 2012 | 22770209 |
empowering biotechnology in southern africa: establishment of a robust transformation platform for the production of transgenic industry-preferred cassava. | knowledge and technology transfer to african laboratories and farmers is an important objective for achieving food security and sustainable crop production on the sub-saharan african continent. cassava (manihot esculenta crantz) is a vital source of calories for more than a billion people in developing countries, and its potential industrial use for starch and bioethanol in the tropics is increasingly being recognized. however, cassava production remains constrained by the susceptibility of the ... | 2013 | 22683498 |
utilization of smoked salmon trim in extruded smoked salmon jerky. | during smoked salmon processing, the dark meat along the lateral line is removed before packaging; this by-product currently has little economic value. in this study, the dark meat trim was incorporated into an extruded jerky. three formulations were processed: 100% smoked trim, 75% : 25% smoked trim : fresh salmon fillet, and 50% : 50% smoked trim : fresh salmon blends (w/w basis). the base formulation contained salmon (approximately 83.5%), tapioca starch (8%), pregelatinized potato starch (3% ... | 2012 | 22591245 |
bio-ethanol production from non-food parts of cassava (manihot esculenta crantz). | global climate issues and a looming energy crisis put agriculture under pressure in sub-saharan africa. climate adaptation measures must entail sustainable development benefits, and growing crops for food as well as energy may be a solution, removing people from hunger and poverty without compromising the environment. the present study investigated the feasibility of using non-food parts of cassava for energy production and the promising results revealed that at least 28% of peels and stems comp ... | 2012 | 22535425 |
violet diode laser-induced chlorophyll fluorescence: a tool for assessing mosaic disease severity in cassava (manihot esculenta crantz) cultivars. | violet diode laser-induced chlorophyll fluorescence was used in agronomical assessment (disease severity and average yield per plant). because cassava (manihot esculenta crantz) is of economic importance, improved cultivars with various levels of affinity for cassava mosaic disease were investigated. fluorescence data correlated with cassava mosaic disease severity levels and with the average yield per plant. | 2012 | 22519123 |
variations in the chemical composition of cassava ( manihot esculenta crantz) leaves and roots as affected by genotypic and environmental variation. | the purpose of this study was to assess the quality of cassava cultivars, in terms of cyanogenic potential and composition of macro- and micronutrients, sampled from different locations in rural mozambique. total cyanide concentrations in fresh cassava tissues were measured using portable cyanide testing kits, and elemental nutrients were later analyzed from dried plant tissue. variation in cyanogenic potential and nutrient composition occurred both among cultivars and across locations. the majo ... | 2012 | 22515684 |
retention of total carotenoid and β-carotene in yellow sweet cassava (manihot esculenta crantz) after domestic cooking. | over the last decade, considerable efforts have been made to identify cassava cultivars to improve the vitamin a nutritional status of undernourished populations, especially in northeast brazil, where cassava is one of the principal and essentially only nutritional source. | 2012 | 22468142 |
reducing the fat content in ground beef without sacrificing quality: a review. | americans are becoming more health conscious in their food choices and many are interested in reducing dietary fat intake. fat replacers can affect meat flavor both by adding flavors of their own, by reducing the original aroma-generating substrate (fat) and by altering release of aroma compounds. when fat is removed from meat, water is generally added to replace it. water-binding compounds can be added to prevent the added water from cooking out or evaporating and to prevent patty shrinkage. fa ... | 2012 | 22444664 |
an est-derived snp and ssr genetic linkage map of cassava (manihot esculenta crantz). | cassava (manihot esculenta crantz) is one of the most important food security crops in the tropics and increasingly being adopted for agro-industrial processing. genetic improvement of cassava can be enhanced through marker-assisted breeding. for this, appropriate genomic tools are required to dissect the genetic architecture of economically important traits. here, a genome-wide snp-based genetic map of cassava anchored in ssrs is presented. an outbreeder full-sib (f1) family was genotyped on tw ... | 2012 | 22419105 |
digestibility, fermentability, and energy value of highly cross-linked phosphate tapioca starch in men. | the objective of this study was to determine glycemic and breath hydrogen responses in 10 healthy men in response to highly cross-linked starch phosphate (hxls), made of tapioca starch (ts). plasma glucose concentration was analyzed at baseline and at 30, 60, 90, 120, 150, and 180 min postprandially. in addition, breath hydrogen excretion was measured at baseline and at hourly intervals, over 10 h, after test substance challenge. when compared with unmodified ts easily digested, the area under t ... | 2011 | 22417501 |
computational identification of micrornas and their targets in cassava (manihot esculenta crantz.). | micrornas (mirnas) are a newly discovered class of noncoding endogenous small rnas involved in plant growth and development as well as response to environmental stresses. mirnas have been extensively studied in various plant species, however, only few information are available in cassava, which serves as one of the staple food crops, a biofuel crop, animal feed and industrial raw materials. in this study, the 169 potential cassava mirnas belonging to 34 mirna families were identified by computat ... | 2013 | 22388699 |
iris melanoma: features and prognosis in 317 children and adults. | to evaluate iris melanoma in children versus adults. | 2012 | 22370659 |
molecular cloning and characterization of a novel ring zinc-finger protein gene up-regulated under in vitro salt stress in cassava. | cassava (manihot esculenta crantz) is one of the world's most important food crops. it is cultivated mainly in developing countries of tropics, since its root is a major source of calories for low-income people due to its high productivity and resistance to many abiotic and biotic factors. a previous study has identified a partial cdna sequence coding for a putative ring zinc finger in cassava storage root. the ring zinc finger protein is a specialized type of zinc finger protein found in many o ... | 2012 | 22307786 |
relationship between population growth of the red flour beetle tribolium castaneum and protein and carbohydrate content in flour and starch. | the effects of eight diets (atta flour, wheat flour, self-rising flour, rice flour, custard powder, corn flour, tapioca starch, and potato starch) on the development of the red flour beetle, tribolium castaneum (herbst), reared at 29-31 degrees c and 66-70% rh were assessed. five pairs of male and female t. castaneum were reared on the respective diets for 28 d before the experimental setup was dismantled and adult counts were recorded. in another experiment, the insects were allowed to mate and ... | 2011 | 22299375 |
hydroxypropylated distarch phosphate versus unmodified tapioca starch: fat oxidation and endurance in c57bl/6j mice. | an rs4-type resistant starch is a chemically modified starch that shows reduced availability in comparison to the corresponding unmodified starch. hydroxypropylated distarch phosphate (hdp) is an rs4-type resistant starch that increases energy expenditure and prevents high-fat diet-induced obesity through increased hepatic fatty acid oxidation. the aim of this study was to clarify the acute effects of hdp from tapioca starch (hpdtsp) on physical performance in mice. male c57bl/6j mice were used ... | 2012 | 22270482 |
genetic structure of traditional varieties of bitter manioc in three soils in central amazonia. | manioc is the most important food crop that originated in amazonia. many studies have increased our understanding of its evolutionary dynamics under cultivation. however, most of them focused on manioc cultivation in environments with low soil fertility, generally oxisols. recent ethnobotanical observations showed that bitter manioc also performs well in high fertility soils, such as amazonian dark earths (ade) and the floodplain. we used 10 microsatellite loci to investigate the genetic diversi ... | 2011 | 22228136 |
iron and protein biofortification of cassava: lessons learned. | over two hundred and fifty million africans rely on the starchy root crop cassava (manihot esculenta) as their primary source of calories. cassava roots, however, have the lowest protein:energy ratio of all the world's major staple crops. furthermore, a typical cassava-based diet provides less than 10-20% of the required amounts of iron, zinc, vitamin a and vitamin e. the biocassava plus program employed modern biotechnologies to improve the health of africans through development and delivery of ... | 2012 | 22226461 |
informal "seed" systems and the management of gene flow in traditional agroecosystems: the case of cassava in cauca, colombia. | our ability to manage gene flow within traditional agroecosystems and their repercussions requires understanding the biology of crops, including farming practices' role in crop ecology. that these practices' effects on crop population genetics have not been quantified bespeaks lack of an appropriate analytical framework. we use a model that construes seed-management practices as part of a crop's demography to describe the dynamics of cassava (manihot esculenta crantz) in cauca, colombia. we quan ... | 2011 | 22174952 |
antioxidant phenolic compounds of cassava (manihot esculenta) from hainan. | an activity-directed fractionation and purification process was used to isolate antioxidant components from cassava stems produced in hainan. the ethyl acetate and n-butanol fractions showed greater dpph˙and abts·+ scavenging activities than other fractions. the ethyl acetate fraction was subjected to column chromatography, to yield ten phenolic compounds: coniferaldehyde (1), isovanillin (2), 6-deoxyjacareubin (3), scopoletin (4), syringaldehyde (5), pinoresinol (6), p-coumaric acid (7), ficuso ... | 2011 | 22157579 |
apomixis in different ploidy levels of cassava. | two polyploid hybrids between cassava (manihot esculenta) cultivar 307-2 and its wild relatives m. glaziovii and m. anomala, were studied to examine the relationship between ploidy level and the production of seeds without fertilization. a clearing method was applied to assess ovule sizes as an indication of multiembryony. the diploid cultivar 307-2 had regular 18 bivalents at meiotic metaphase 1 while the polyploid types showed chromosome configurations varying from 3 to 4 quadrivalents and 28 ... | 2011 | 22150824 |
preparation and characterization of new and improved soluble-starches, -amylose, and -amylopectin by reaction with benzaldehyde/zinc chloride. | seven different starches from potato, rice, maize, waxymaize, amylomaize-vii, shoti, and tapioca, and potato amylose and potato amylopectin have been reacted with benzaldehyde, catalyzed by zncl(2), to give new water-soluble starches and water soluble-amylose and soluble-amylopectin. in contrast to the native starches, aqueous solutions of the modified starches could not be precipitated with 2-, 3-, or 4-volumes of ethanol. β-amylase gave no reaction with the modified starches, in contrast to th ... | 2011 | 22055814 |
marriage exchanges, seed exchanges, and the dynamics of manioc diversity. | the conservation of crop genetic resources requires understanding the different variables-cultural, social, and economic-that impinge on crop diversity. in small-scale farming systems, seed exchanges represent a key mechanism in the dynamics of crop genetic diversity, and analyzing the rules that structure social networks of seed exchange between farmer communities can help decipher patterns of crop genetic diversity. using a combination of ethnobotanical and molecular genetic approaches, we inv ... | 2011 | 22042843 |
a new diterpene from the stems of manihot esculenta. | a new diterpene, named yucalexin p-23 (1), together with three known compounds, yucalexin p-15 (2), protocatechuic acid (3), and catalpinic acid (4), was isolated from the stems of manihot esculenta. their structures were determined by means of physicochemical evidence and spectral analysis. compounds 3 and 4 exhibited antimicrobial activity. | 2011 | 21972813 |
a web accessible resource for investigating cassava phenomics and genomics information: biogen base. | the goal of our research is to establish a unique portal to bring out the potential outcome of the research in the casssava crop. the biogen base for cassava clearly brings out the variations of different traits of the germplasms, maintained at the tapioca and castor research station, tamil nadu agricultural university. phenotypic and genotypic variations of the accessions are clearly depicted, for the users to browse and interpret the variations using the microsatellite markers. database (bioge ... | 2011 | 21904428 |
allergic reactions to manioc (manihot esculenta crantz): identification of novel allergens with potential involvement in latex-fruit syndrome. | | 2011 | 21855976 |
physicochemical properties of monosodium glutamate-compounded tapioca starch exceeds those of simple heat-moisture treated starch. | monosodium glutamate (gluna)-compounded starch was prepared by heat-moisture treating a mixture of tapioca starch and gluna. gluna-compounded starch exhibited a higher gelatinization temperature and reduced swelling and solubility, essentially lower hardness of the granule center, and paste viscosity than those of the heat-moisture treated tapioca starch and the untreated starch. however, its appearance, unit chain length distribution, and α-amylase digestibility were similar to those of the hea ... | 2011 | 21806607 |
isolation and partial characterization of a root-specific promoter for stacking multiple traits into cassava (manihot esculenta crantz). | cassava can be cultivated on impoverished soils with minimum inputs, and its storage roots are a staple food for millions in africa. however, these roots are low in bioavailable nutrients and in protein content, contain cyanogenic glycosides, and suffer from a very short post-harvest shelf-life, and the plant is susceptible to viral and bacterial diseases prevalent in africa. the demand for improvement of cassava with respect to these traits comes from both farmers and national agricultural inst ... | 2011 | 21710453 |
anatomic changes due to interspecific grafting in cassava (manihot esculenta). | cassava rootstocks of varieties unb 201 and unb 122 grafted with scions of manihot fortalezensis were prepared for anatomic study. the roots were cut, stained with safranin and alcian blue, and examined microscopically, comparing them with sections taken from ungrafted roots. there was a significant decrease in number of pericyclic fibers, vascular vessels and tyloses in rootstocks. they exhibited significant larger vessels. these changes in anatomic structure are a consequence of genetic effect ... | 2011 | 21710451 |
genomic clustering of cyanogenic glucoside biosynthetic genes aids their identification in lotus japonicus and suggests the repeated evolution of this chemical defence pathway. | cyanogenic glucosides are amino acid-derived defence compounds found in a large number of vascular plants. their hydrolysis by specific β-glucosidases following tissue damage results in the release of hydrogen cyanide. the cyanogenesis deficient1 (cyd1) mutant of lotus japonicus carries a partial deletion of the cyp79d3 gene, which encodes a cytochrome p450 enzyme that is responsible for the first step in cyanogenic glucoside biosynthesis. the genomic region surrounding cyp79d3 contains genes en ... | 2011 | 21707799 |
transfer factor of the radionuclides in food crops from high-background radiation area of south west india. | it is necessary to obtain the transfer factor (tf) of long-lived radionuclides because soil type and vegetation can affect tf. we studied the food crops commonly consumed by the general public of kanyakumari district of south india. the main focus was on rice, fruits, vegetables and tapioca because the consumption of these is high. the soil to rice tf for the radionuclides, (226)ra, (232)th, (238)u and (40) k are 8.8×10(-2), 14.2×10(-2), 5.8×10(-2) and 6.3×10(-2), respectively. the tf of tapioca ... | 2012 | 21680604 |
a survey of total hydrocyanic acid content in ready-to-eat cassava-based chips obtained in the australian market in 2008. | cassava (manihot esculenta crantz) is a widely consumed food in the tropics that naturally contains cyanogenic glycosides (cyanogens, mainly composed of linamarin, acetone cyanohydrin, and hydrocyanic acid). if cassava is not adequately processed to reduce the level of cyanogens prior to consumption, these compounds can lead to the formation of hydrocyanic acid in the gut. exposure to hydrocyanic acid can cause symptoms ranging from vomiting and abdominal pain to coma and death. in 2008, a surve ... | 2011 | 21669076 |
a genome scan for quantitative trait loci affecting cyanogenic potential of cassava root in an outbred population. | cassava (manihot esculenta crantz) can produce cyanide, a toxic compound, without self-injury. that ability was called the cyanogenic potential (cn). this project aimed to identify quantitative trait loci (qtl) associated with the cn in an outbred population derived from 'hanatee' × 'huay bong 60', two contrasting cultivars. cn was evaluated in 2008 and in 2009 at rayong province, and in 2009 at lop buri province, thailand. cn was measured using a picrate paper kit. qtl analysis affecting cn was ... | 2011 | 21609492 |
changes in scopoletin concentration in cassava chips from four varieties during storage. | the use of the root crop cassava (manihot esculenta crantz) is constrained by its rapid deterioration after harvesting. chemical and spectroscopic examination earlier revealed the accumulation of the four hydroxycoumarins esculetin, esculin, scopolin and scopoletin derived from the phenylpropanoid pathway, during the time course of postharvest deterioration. in this investigation the scopoletin level in parenchymal samples of four cassava cultivars used in benin, i.e. kpaki kpika, kpaki soan, lo ... | 2011 | 21604276 |
cassava genetic transformation and its application in breeding. | as a major source of food, cassava (manihot esculenta crantz) is an important root crop in the tropics and subtropics of africa and latin america, and serves as raw material for the production of starches and bioethanol in tropical asia. cassava improvement through genetic engineering not only overcomes the high heterozygosity and serious trait separation that occurs in its traditional breeding, but also quickly achieves improved target traits. since the first report on genetic transformation in ... | 2011 | 21564542 |
nutrition in transition: dietary patterns of rural amazonian women during a period of economic change. | the goal of this study was to understand the relationship between economic change (wage labor, retirement, and the bolsa família program) and dietary patterns in the rural amazon and to determine the extent to which these changes followed the pattern of the nutrition transition. | 2011 | 21538648 |
moisture-pressure combination treatments for cyanide reduction in grated cassava. | several cyanide-associated health disorders have been linked with frequent consumption of mildly toxic cassava (manihot esculenta crantz) products in individuals on a low-protein diet. production of bread from cassava often involves application of prolonged physical pressure (pressing) to the freshly grated root for several hours. this study aimed to determine effects of pressure and wetting on grated cassava. six treatments were applied: confining pressure for 12 h, wetting for 4 h at 25 °c, 2 ... | 2013 | 21535726 |
effect of powder and target properties on wrap around effect during coating. | tapioca starch, nacl (28, 135, and 378 μm), corn starch, cocoa powder, soy protein isolate, cheese powder, wheat protein, modified starch, nacho cheese, and sugar were coated at 0 kv for nonelectrostatic and at 25 kv for electrostatic coating onto metal, wood, unoiled paper, oiled paper, unoiled plastic, oiled plastic, fresh bread, and dry bread. powders and targets were allowed to naturally tribocharge, or all charge was removed before coating. powder particle size, flowability, resistivity, an ... | 2010 | 21535493 |
the biocassava plus program: biofortification of cassava for sub-saharan africa. | more than 250 million africans rely on the starchy root crop cassava (manihot esculenta) as their staple source of calories. a typical cassava-based diet, however, provides less than 30% of the minimum daily requirement for protein and only 10%-20% of that for iron, zinc, and vitamin a. the biocassava plus (bc+) program has employed modern biotechnologies intended to improve the health of africans through the development and delivery of genetically engineered cassava with increased nutrient (zin ... | 2011 | 21526968 |
primary anal mucinous adenocarcinoma: a case series. | primary mucinous adenocarcinoma is a rare malignancy of the anus (3% of anal cancers), the pathogenesis of which is better understood by immunohistochemistry. we reviewed cases of primary mucinous adenocarcinoma of the anus from 2000 to 2009 in the hepatogastroenterology department of the university hospital centre of marrakech. three cases were identified who were males with the mean age of 68years, and presented with a chronic anal fistula. two cases had anal pain. clinical examination reveale ... | 2011 | 21429457 |
leaf proteomic analysis in cassava (manihot esculenta, crantz) during plant development, from planting of stem cutting to storage root formation. | tuberization in cassava (manihot esculenta crantz) occurs simultaneously with plant development, suggesting competition of photoassimilate partitioning between the shoot and the root organs. in potato, which is the most widely studied tuber crop, there is ample evidence suggesting that metabolism and regulatory processes in leaf may have an impact on tuber formation. to search for leaf proteins putatively involved in regulating tuber generation and/or development in cassava, comparative proteomi ... | 2011 | 21327816 |
neurological disorders associated with cassava diet: a review of putative etiological mechanisms. | tropical ataxic neuropathy (tan) and epidemic spastic paraparesis (konzo) are two neurological disorders associated with the consumption of cassava (manihot esculenta) in several african countries. tan is characterized by sensory polyneuropathy, sensory ataxia, bilateral optic atrophy and bilateral sensori-neural deafness. it occurs in elderly individuals subsisting on a monotonous cassava diet with minimal protein supplementation. konzo is a syndrome of symmetrical spastic paraparesis with a pr ... | 2011 | 21327546 |
transgenic biofortification of the starchy staple cassava (manihot esculenta) generates a novel sink for protein. | although calorie dense, the starchy, tuberous roots of cassava provide the lowest sources of dietary protein within the major staple food crops (manihot esculenta crantz). (montagnac ja, davis cr, tanumihardjo sa. (2009) compr rev food sci food saf 8:181-194). cassava was genetically modified to express zeolin, a nutritionally balanced storage protein under control of the patatin promoter. transgenic plants accumulated zeolin within de novo protein bodies localized within the root storage tissue ... | 2011 | 21283593 |
ssr and est-ssr-based genetic linkage map of cassava (manihot esculenta crantz). | simple sequence repeat (ssr) markers provide a powerful tool for genetic linkage map construction that can be applied for identification of quantitative trait loci (qtl). in this study, a total of 640 new ssr markers were developed from an enriched genomic dna library of the cassava variety 'huay bong 60' and 1,500 novel expressed sequence tag-simple sequence repeat (est-ssr) loci were developed from the genbank database. to construct a genetic linkage map of cassava, a 100 f(1) line mapping pop ... | 2011 | 21222095 |
cassava: constraints to production and the transfer of biotechnology to african laboratories. | knowledge and technology transfer to african institutes is an important objective to help achieve the united nations millennium development goals. plant biotechnology in particular enables innovative advances in agriculture and industry, offering new prospects to promote the integration and dissemination of improved crops and their derivatives from developing countries into local markets and the global economy. there is also the need to broaden our knowledge and understanding of cassava as a sta ... | 2011 | 21212961 |
analysis of heterogeneity of copia-like retrotransposons in the genome of cassava (manihot esculenta crantz). | retrotransposons are ubiquitous in eukaryotic genomes and now proving to be useful genetic tools for genetic diversity and phylogenetic analyses, especially in plants. in order to assess the diversity of ty1/copia-like retrotransposons of cassava, we used pcr primers anchored on the conserved domains of reverse transcriptases (rts) to amplify cassava ty1/copia-like rt. the pcr product was cloned and sequenced. sequences analysis of the clones revealed the presence of 69 families of ty1/copia-lik ... | 2011 | 22547180 |
resistance to the whitefly, aleurotrachelus socialis, in wild populations of cassava, manihot tristis. | the levels of resistance in the wild species of cassava, manihot tristis muell-arg. (malpighiales: euphorbiaceae), to the whitefly, aleurotrachelus socialis bondar (hemiptera: alelyrodidae), the most important economic pest in cassava, manihot esculenta crantz (malpighiales: euphorbiaceae) crops in south america, were estimated under glasshouse conditions. the parameters of the life history of a. socialis were studied on tst-26 and tst-18 accessions of the wild parent and compared with the susce ... | 2010 | 21062208 |
evaluation of bioenergy recovery processes treating organic residues from ethanol fermentation process. | this study evaluates a two-stage bioprocess for recovering bioenergy in the forms of hydrogen and methane while treating organic residues of ethanol fermentation from tapioca starch. a maximum hydrogen production rate of 0.77 mmol h(2)/g vss/h can be achieved at volumetric loading rate (vlr) of 56 kg cod/m(3)/day. batch results indicate that controlling conditions at s(0)/x(0)=12 with x(0)=4000 mg vss/l and ph 5.5-6 are important for efficient hydrogen production from fermentation residues. hydr ... | 2011 | 21055919 |
quantification of organic and nitrogen removal in downflow hanging sponge (dhs) systems as a post-treatment of uasb effluent. | the aim of this research was to investigate the nature and composition of organic substrate in two down-flow hanging sponge (dhs) systems using mixed fungal (fdhs) and bacterial (bdhs) cultures treatment for uasb effluent of tapioca starch wastewater, evaluated by cod fractionations and two material balances. the random type dhs reactors were operated as modular columns consisting of four identical segments connected vertically. results of the wastewater characterization showed that carbonaceous ... | 2010 | 21045340 |
tapioca starch graft copolymers and dome matrix® modules ii. effect of modules assemblage on riboflavin release kinetics. | this paper studies the riboflavin release from systems made of assembled modules of dome matrix® technology using tapioca starch-ethylmethacrylate (tsema) and tapioca hydroxypropylstarch-ethylmethacrylate (thsema) graft copolymers produced by two different drying methods. two different shape modules were manufactured for this study, i.e., female and male modules, in order to facilitate their assemblage in "void configuration", a system with an internal void space. drug release studies on void co ... | 2011 | 20974252 |
an ordered est catalogue and gene expression profiles of cassava (manihot esculenta) at key growth stages. | a cdna library was constructed from the root tissues of cassava variety huanan 124 at the root bulking stage. a total of 9,600 cdna clones from the library were sequenced with single-pass from the 5'-terminus to establish a catalogue of expressed sequence tags (ests). assembly of the resulting est sequences resulted in 2,878 putative unigenes. blastn analysis showed that 62.6% of the unigenes matched with known cassava ests and the rest had no 'hits' against the cassava database in the integrati ... | 2010 | 20957510 |
cassava: an appraisal of its phytochemistry and its biotechnological prospects. | the present state of knowledge of the phytochemistry of small molecules isolated from the roots and leaves of cassava, manihot esculenta crantz (euphorbiaceae), is reviewed. cassava roots are an important source of dietary and industrial carbohydrates, mainly eaten as a source of starch, forming the staple food to over 500 million; additionally, the roots have value as a raw material for industrial starch production and for animal feed giving the crop high economic value, but it suffers markedly ... | 2010 | 20943239 |
hydrolysis/dehydration/aldol-condensation/hydrogenation of lignocellulosic biomass and biomass-derived carbohydrates in the presence of pd/wo3-zro2 in a single reactor. | hydrolysis/dehydration/aldol-condensation/hydrogenation of lignocellulosic-biomass (corncobs) and biomass-derived carbohydrates (tapioca flour) to produce water-soluble c5-c15 compounds was developed in a single reactor system. wo3-zro2 efficiently catalyzed the hydrolysis/dehydration of these feedstocks to 5-hydroxymethylfurfural and furfural, while the impregnation of wo3-zro2 with pd allowed sequential aldolcondensation/hydrogenation of these furans to c5-c15 compounds. the highest c5-c15 yie ... | 2011 | 20934873 |
application of microbial α-amylase in industry - a review. | amylases are one of the main enzymes used in industry. such enzymes hydrolyze the starch molecules into polymers composed of glucose units. amylases have potential application in a wide number of industrial processes such as food, fermentation and pharmaceutical industries. α-amylases can be obtained from plants, animals and microorganisms. however, enzymes from fungal and bacterial sources have dominated applications in industrial sectors. the production of α-amylase is essential for conversion ... | 2010 | 24031565 |
kinetics of thermal softening of cassava tubers and rheological modeling of the starch. | cassava or tapioca (manihot esculenta crantz) tubers having high amount of carbohydrate are utilized after boiling or processing into starch and flour. textural properties of raw and cooked tubers depend on variety, maturity, growing environment, physico-chemical and starch properties. starch is used in food preparations as gelling and thickening agent, stabilizer and texture modifier. this study aims at analyzing and modeling the textural, dynamic rheological and gelatinization properties of se ... | 2010 | 23572679 |
movement behaviour within and beyond perceptual ranges in three small mammals: effects of matrix type and body mass. | 1. for animal species inhabiting heterogeneous landscapes, the tortuosity of the dispersal path is a key determinant of the success in locating habitat patches. path tortuosity within and beyond perceptual range must differ, and may be differently affected by intrinsic attributes of individuals and extrinsic environmental factors. understanding how these factors interact to determine path tortuosity allows more accurate inference of successful movements between habitat patches. 2. we experimenta ... | 2010 | 20707824 |
etiology of konzo, epidemic spastic paraparesis associated with cyanogenic glycosides in cassava: role of thiamine deficiency? | konzo is a syndrome of symmetrical, non-progressive, non-remitting spastic paraparesis occurring in epidemic and endemic forms in several countries in africa, invariably associated with monotonous consumption of inadequately processed bitter cassava roots (manihot esculenta) with very minimal protein supplementation. despite numerous epidemiological, clinical and biochemical studies by authors in several countries aimed at elucidating the etiological mechanisms of konzo, the etiology remains unk ... | 2010 | 20619859 |
senescence-inducible expression of isopentenyl transferase extends leaf life, increases drought stress resistance and alters cytokinin metabolism in cassava. | cassava (manihot esculenta crantz) sheds its leaves during growth, especially within the tropical dry season. with the production of sag12-ipt transgenic cassava we want to test the level of leaf retention and altered cytokinin metabolism of transgenic plants via the autoregulatory senescence inhibition system. after confirmation of transgene expression by molecular analysis and phenotype examination in greenhouse plants, two transgenic plant lines, 529-28 and 529-48, were chosen for further inv ... | 2010 | 20590995 |
aflp-based transcript profiling for cassava genome-wide expression analysis in the onset of storage root formation. | cassava (manihot esculenta crantz) is a root crop that accumulates large quantities of starch, and it is an important source of carbohydrate. study on gene expressions during storage root development provides important information on storage root formation and starch accumulation as well as unlock new traits for improving of starch yield. cdna-amplified fragment length polymorphism (aflp) was used to compare gene expression profiles in fibrous and storage roots of cassava cultivar kasetsart 50. ... | 2010 | 20536786 |
alterations of reproduction system in a polyploidized cassava interspecific hybrid. | the objective of this research was to examine how much polyploidy may affect seed and root formation in cassava interspecific hybrids manihot esculenta crantz xm. oligantha pax. a polyploid type was induced by colchicine treatment to lateral buds followed by propagating vegetatively arising stems. cytogenetic and anatomical analyses were made on both polyploid and diploid types. the polyploid type showed extensive chromosome pairing and pollen viability. multiembryonic ovule frequency increased ... | 2010 | 20536543 |
effect of different binders on the physico-chemical, textural, histological, and sensory qualities of retort pouched buffalo meat nuggets. | the functional properties of 4 binders, namely corn starch, wheat semolina, wheat flour, and tapioca starches, were evaluated to improve the quality of buffalo meat nuggets processed in retort pouches at f(0) 12.13. incorporation of corn starch in buffalo meat nuggets produced more stable emulsion than other binders used. product yield, drip loss, and ph did not vary significantly between the products with different binders. shear force value was significantly higher for product with corn starch ... | 2016 | 20492199 |
sequestering of cu(ii) from aqueous solution using cassava peel (manihot esculenta). | cassava peel is a prospective cheap biosorbent for metal ions sequestration. in this research, the ability of cassava peel to remove cu(ii) from aqueous solution was evaluated. its physical characteristics were probed by nitrogen adsorption measurements and scanning electron microscopy while its biosorption mechanism was studied by fourier transform infrared (ftir), scanning electron microscopy/energy dispersive using x-ray analysis-(sem/edx), x-ray mapping and x-ray photoelectron spectroscopy ( ... | 2010 | 20471169 |
cassava root diet induces low pyruvate levels. | the high cyanogenic-glucoside carbohydrate of the cassava root (manihot esculenta) has special properties that make it an ideal therapeutic food for lowering nicotinamide adenine dinucleotide reduced form (nadh) and inducing sirtuin (sirt) gene overexpression when eaten in an exclusive mono-food diet regime. the author, using himself as the sole test subject, repeatedly induced low pyruvate levels (reflective of nadh levels) after being on the diet for 1-2 weeks at a time. the possible influence ... | 2013 | 20462383 |
enhancer/suppressor mutator (en/spm)-like transposable elements of cassava (manihot esculenta) are transcriptionally inactive. | transposable elements contribute to the size, structure, variation, and diversity of the genome and have major effects on gene function. sequencing projects have revealed the diversity of transposable elements in many organisms and have shown that they constitute a high percentage of the genome. pcr-based techniques using degenerate primers designed from conserved enzyme domains of transposable elements can provide quick and extensive surveys, making study of diversity and abundance and their ap ... | 2010 | 20449796 |
genetic, embryonic and anatomical study of an interspecific cassava hybrid. | a molecular, anatomical and cytogenetic study of an interspecific hybrid between manihot esculenta (cassava) and the wild species m. oligantha was carried out. cytogenetics revealed relatively complete chromosome pairing and high viability of the pollen grains. ovule structure examined by the clearing method showed polyembryony in 2.7% of the ovules. doubling of the chromosome number resulted in an increase in polyembryony of up to 18% and a reduction in pollen viability. multivalent formation w ... | 2010 | 20391337 |
expression pattern conferred by a glutamic acid-rich protein gene promoter in field-grown transgenic cassava (manihot esculenta crantz). | a major constraint for incorporating new traits into cassava using biotechnology is the limited list of known/tested promoters that encourage the expression of transgenes in the cassava's starchy roots. based on a previous report on the glutamic-acid-rich protein pt2l4, indicating a preferential expression in roots, we cloned the corresponding gene including promoter sequence. a promoter fragment (cp2; 731 bp) was evaluated for its potential to regulate the expression of the reporter gene gusplu ... | 2010 | 20336312 |
the evolutionary ecology of clonally propagated domesticated plants. | while seed-propagated crops have contributed many evolutionary insights, evolutionary biologists have often neglected clonally propagated crops. we argue that widespread notions about their evolution under domestication are oversimplified, and that they offer rich material for evolutionary studies. the diversity of their wild ancestors, the diverse ecologies of the crop populations themselves, and the intricate mix of selection pressures, acting not only on the parts harvested but also on the pa ... | 2010 | 20202131 |
proteome characterization of cassava (manihot esculenta crantz) somatic embryos, plantlets and tuberous roots. | proteomics is increasingly becoming an important tool for the study of many different aspects of plant functions, such as investigating the molecular processes underlying in plant physiology, development, differentiation and their interaction with the environments. to investigate the cassava (manihot esculenta crantz) proteome, we extracted proteins from somatic embryos, plantlets and tuberous roots of cultivar sc8 and separated them by sodium dodecyl sulfate polyacrylamide gel electrophoresis ( ... | 2010 | 20187967 |
constituents and secondary metabolite natural products in fresh and deteriorated cassava roots. | a phytochemical analysis of cassava (manihot esculenta crantz) fresh roots and roots suffering from post-harvest physiological deterioration (ppd) has been carried out. the first isolation and identification of galactosyl diacylglycerides from fresh cassava roots is reported, as well as beta-carotene, linamarin, and beta-sitosterol glucopyranoside. the hydroxycoumarin scopoletin and its glucoside scopolin were identified from cassava roots during ppd, as well as trace quantities of esculetin and ... | 2010 | 20137795 |
a reliable and efficient method for total rna isolation from various members of spurge family (euphorbiaceae). | it is prerequisite and crucial to extract rna with high quality and integrity in order to carry out molecular biology studies in any plant species of a family. euphorbiaceae members are known for high levels of their waxes, oils with polysaccharides, polyphenolics and secondary metabolites. these conditions are recognised to interfere unfavourably with various methodologies of rna isolation. | 2012 | 20135710 |