| clinical value of the measurement of mycobacterium tuberculosis specific antibody in pulmonary tuberculosis. | a serological test that could help to diagnose tuberculosis, especially smear negative disease, would contribute to patient management. | 1992 | 1585290 |
| a search for mycobacterial dna in granulomatous tissues from patients with sarcoidosis using the polymerase chain reaction. | we have used the polymerase chain reaction as a tool to detect the presence of mycobacterial dna from organisms of the mycobacterium tuberculosis complex and other species of mycobacteria in samples from patients with sarcoidosis. using systems based on the amplification of a fragment of the gene coding for the 65-kd mycobacterial antigen, which were demonstrated to detect approximately 20 mycobacterial genomes/microgram total dna, dna from m. tuberculosis was reproducibly identified in dna extr ... | 1992 | 1586060 |
| pyrazinamide sterilizing activity in vitro against semidormant mycobacterium tuberculosis bacterial populations. | previously, we reported that pyrazinamide has very poor bactericidal activity against m. tuberculosis growing in broth at ph 5.6. in the present study, cultivation at ph 4.8 to 5.0 in 7h12 broth prevented an increase in the number of viable bacteria, but the cultures remained metabolically active. the presence of 50 micrograms/ml pyrazinamide in semidormant cultures led to a sharp decline in the number of viable bacteria, by more than 1,000-fold. this unfavorable environment probably made the ba ... | 1992 | 1586071 |
| purification and characterization of an unusually large fatty acid synthase from mycobacterium tuberculosis var. bovis bcg. | fatty acid synthase was purified from mycobacterium tuberculosis var. bovis bcg. the method developed gave a 23% yield of the synthase and also yielded purified mycocerosic acid synthase. the fatty acid synthase is of unusually large size and composed of two 500-kda monomers. the amino acid composition of the two synthases was not identical; the n-terminus of the fatty acid synthase was blocked, whereas that of the mycocerosic acid synthase was not. western blot analysis of crude mycobacterial e ... | 1992 | 1586161 |
| cloning and sequencing of pseudomonas genes determining sodium dodecyl sulfate biodegradation. | the nucleotide sequences of two genes involved in sodium dodecyl sulfate (sds) degradation, by pseudomonas, have been determined. one of these, sdsa, codes for an alkyl sulfatase (58,957 da) and has similarity (31.8% identity over a 201-amino acid stretch) to the n terminus of a predicted protein of unknown function from mycobacterium tuberculosis. the other gene, sdsb, codes for a positive activator protein (33,600 da) that has extensive similarity with the lysr family of helix-turn-helix dna-b ... | 1992 | 1587481 |
| alterations in the superoxide dismutase gene of an isoniazid-resistant strain of mycobacterium tuberculosis. | genetic analysis of a set of six mycobacterium tuberculosis strains differing in virulence for the guinea pig revealed an altered restriction enzyme fragmentation pattern associated with the superoxide dismutase (sod) gene in a low-virulence, isoniazid-resistant strain. in addition, it was found that the sod enzyme produced by the isoniazid-resistant strain differed in its electrophoretic mobility from the sod of other m. tuberculosis strains. detailed analysis of these strain-specific differenc ... | 1992 | 1587584 |
| structure and function of a 40,000-molecular-weight protein antigen of mycobacterium tuberculosis. | a gene encoding a protein antigen from mycobacterium tuberculosis with a molecular weight of 40,000 has been sequenced. on the basis of sequence homology and functional analyses, we demonstrated that the protein is an l-alanine dehydrogenase (ec 1.4.1.1). the enzyme was demonstrated in m. tuberculosis and mycobacterium marinum but not in mycobacterium bovis bcg. the enzyme may play a role in cell wall synthesis because l-alanine is an important constituent of the peptidoglycan layer. although no ... | 1992 | 1587598 |
| recovery of adrenocortical function following treatment of tuberculous addison's disease. | we report two cases of addison's disease occurring in association with proven mycobacterium tuberculosis infection. both were treated with modern chemotherapeutic agents and were shown to have developed normal adrenal cortical function after several years. we suggest that adrenal function should be tested after modern anti-tuberculous therapy of this disease. | 1992 | 1589379 |
| tuberculosis rebounding. | | 1992 | 1589784 |
| [treatment of urogenital tuberculosis with ofloxacin. preliminary study]. | ofloxacin is a new fluoride quinolone which has been shown to be active both in vitro and in vivo against mycobacterium tuberculosis. this prospective study presents the results obtained with a new protocol for the treatment of genito-urinary tuberculosis. the comparison drugs were ofloxacin 200 mg every 12 hours for 6 months, rifampicin 600 mg every 24 for 3 months and isoniazid 300 mg every 24 hours for 3 months. the results, were clinically and microbiologically good. it is concluded that thi ... | 1992 | 1590076 |
| evaluation of conventional and radiometric fecal culture and a commercial dna probe for diagnosis of mycobacterium paratuberculosis infections in cattle. | radiometric (rcm) and conventional fecal culture (hey) and a commercial polymerase chain reaction/dna probe were evaluated as diagnostic tests for subclinical paratuberculosis in dairy cattle using fecal specimens from a repository of paratuberculosis specimens. the case definition of subclinical bovine paratuberculosis was isolation of mycobacterium paratuberculosis, by conventional or radiometric culture, from fecal samples or internal organs of dairy cattle without diarrhea or chronic weight ... | 1992 | 1591658 |
| tuberculosis in apparently healthy milch cows. | screening for mycobacteria was carried out on samples of normal milk, supramammary lymph nodes (slns) and udder tissue from apparently healthy milch cows, including the milk from those suspected of tuberculous mastitis. milk samples from the slaughtered and live fulani cows which were apparently healthy yielded 5% and 7% of tubercle bacilli, respectively, while suspected tuberculous milk from slaughtered cows and live cows yielded 8% and 10% of the bacilli, respectively. tubercle bacilli were is ... | 1992 | 1593961 |
| tuberculosis and the haemopoietic system. | | 1992 | 1596588 |
| spectrum of tuberculosis in patients with hiv infection in british columbia: report of 40 cases. | to review the clinical features, treatment and outcome of all known cases of tuberculosis in patients with human immunodeficiency virus (hiv) infection in british columbia between 1984 and 1990. | 1992 | 1596841 |
| detection of mycobacteria using the polymerase chain reaction. | | 1992 | 1597210 |
| tuberculosis: a global overview of the situation today. | the overall tuberculosis situation in the world in 1990 and its recent trends are reviewed by an analysis of the case notifications to who and tuberculosis mortality reports. estimates of the prevalence of tuberculosis infection and the incidence of tuberculosis disease and deaths predicted in 1990 were carried out with simple epidemiological models. approximately one third of the world's population is infected with mycobacterium tuberculosis. in the past decade, an average of 2.5 to 3.2 million ... | 1992 | 1600578 |
| pulmonary eosinophilia in pulmonary tuberculosis. | three radiologically and bacteriologically confirmed pulmonary tuberculosis patients had eosinophilic pneumonia, as demonstrated by bal. in two patients, pulmonary eosinophilia was present only at the site of the lesion and the third had eosinophilia in both peripheral blood and lung. there was complete elimination of the eosinophilic inflammatory process in two patients who had successfully completed antituberculosis treatment. | 1992 | 1600796 |
| mycobacterial heat-shock proteins as carrier molecules. ii: the use of the 70-kda mycobacterial heat-shock protein as carrier for conjugated vaccines can circumvent the need for adjuvants and bacillus calmette guérin priming. | in a recent work, we have shown that mycobacterial heat-shock proteins (hsp) of 65-kda (groel-type) and 70-kda (dnak-type) acted as carrier molecules in mice, previously primed with mycobacterium tuberculosis var. bovis (bacillus calmette-guérin, bcg), for the induction of high and long-lasting titers of igg against the repetitive malaria synthetic peptide (nanp)40. anti-peptide antibodies were induced when the malaria peptide, conjugated to the mycobacterial hsp, was given in the absence of any ... | 1992 | 1601031 |
| secretion of interleukin-8 following phagocytosis of mycobacterium tuberculosis by human monocyte cell lines. | tuberculosis is a major cause of mortality worldwide and incidence is increasing as a result of the aids epidemic. cytokines such as tumor necrosis factor (tnf) are important in the host response to mycobacterium tuberculosis. tnf is involved in both granuloma formation and has direct anti-mycobacterial activity. this study investigated the secretion of interleukin (il)-8 following phagocytosis of m. tuberculosis by a human monocytic cell line and by a more phenotypically mature macrophage-like ... | 1992 | 1601032 |
| bcg vaccination in malnourished child population. | the efficacy of bcg vaccination is still a subject of controversy. in the present study the protective role of bcg vaccination, the influence of nutritional status and justification for revaccination in children were investigated. of the 504 preschool children suffering from tuberculosis who were registered for the study, 345 children did not receive bcg vaccine while the others had it during early infancy. vaccinated children showed a significantly greater tendency to localise the tubercular le ... | 1992 | 1601494 |
| rapid diagnosis of tuberculous meningitis. | | 1992 | 1602154 |
| assessment of the diagnostic value of the native pgltb1, its synthetic neoglycoconjugate pgltb0 and the sulfolipid iv antigens for the serodiagnosis of tuberculosis. | a major phenolic glycolipid (pgltb1) from mycobacterium tuberculosis, that resembles the phenolic glycolipid-i (pgl-i) from m. leprae, and its synthetic terminal diglycosyl conjugate (pgltb0) were reported and raised the prospects of a specific serodiagnostic test for tuberculosis (tb). the diagnostic use of a sulfolipid, namely the sliv, was also reported. the objective of this investigation was to assess the relative sensitivity, specificity, and predictive values of three elisas using the pgl ... | 1992 | 1602185 |
| does previous bcg vaccination interfere with the serodiagnosis of tuberculosis using mycobacterium tuberculosis-specific glycolipid antigens? | | 1992 | 1602200 |
| [rapid diagnosis of tuberculosis by amplification of mycobacterial dna in blood diseases]. | in patients with malignant blood disease, infection is the most serious complication. the prompt differential diagnosis of tuberculosis leads to an appropriate treatment. a method based on dna amplification and hybridization for the rapid detection of mycobacterium tuberculosis was used to test 10 clinical specimens (sputum, gastric aspirate and pleural effusion) from blood disease patients in whom tuberculosis was suspected. mycobacterium tuberculosis dna was detected in 3 specimens, including ... | 1992 | 1602603 |
| [analysis of chronic excretors of mycobacterium bacilli in aichi prefecture]. | among the patients diagnosed as having pulmonary tuberculosis who were newly admitted to six major tuberculosis centers in aichi prefecture during the period from january 1, 1982 to december 31, 1986, 73 patients were chronic excretors of mycobacterium bacilli (chronics), whose sputum cultures tested positive at both 11 and 12 months after admission. in this study, those 73 patients were analyzed in march 1988. if the patient was still hospitalized at that time, the patient was re-evaluated six ... | 1992 | 1602726 |
| [tuberculous meningitis with atypical presentation in a patient with human immunodeficiency virus infection]. | a 32 years old male patient is described with infection by the human immunodeficiency virus (hiv) on stage iv c1 and with positive ag p24 who developed tuberculous meningitis of atypical presentation. a persistent liquoral neutrophilia and low adenosindeaminase values were observed in cerebrospinal fluid of purulent appearance. the patient responded badly to tuberculostatic treatment and died. in the antibiogram carried out resistance to mycobacterium tuberculosis was observed to rifampicine and ... | 1992 | 1602855 |
| [2 complications of primary pulmonary tuberculosis in children]. | | 1992 | 1603151 |
| [effects of low-intensity garnet laser irradiation on microorganisms and wounds]. | the influence of low-intensity garnet laser radiation a wavelength of 0.53 microns (green spectrum) on mycobacterium tuberculosis (mt), nonspecific microflora and regeneration of aseptic and purulent wounds has been studied experimentally for the first time. irradiation of mt and nonspecific microflora inoculations has shown that this type of laser radiation fails to stimulate growth of mt and nonspecific microflora and has an inhibiting action on mt manifested by delayed mt growth, a lower numb ... | 1992 | 1603794 |
| m. tuberculosis-complex specific t-cell stimulation and dth reactions induced with a peptide from the 38-kda protein. | an immunodominant t-cell-stimulatory epitope located near the carboxy terminus of the 38-kda antigen from m. tuberculosis (38.g, residues 350-369) was found to be m. tuberculosis-complex specific. this was demonstrated by the presence of proliferative and delayed type hypersensitivity (dth) responses in mice immunized with mycobacterium tuberculosis and mycobacterium bovis bcg, whereas mice immunized with m. avium or other non-tuberculous species of mycobacteria showed no such responses. peptide ... | 1992 | 1604242 |
| tuberculosis in the 1990's: resurgence, regimens, and resources. | physicians in the united states must maintain vigilance for the 25,000 annual new cases of tuberculosis, concentrated in the elderly, in immigrants, in migrant and minority populations, and in immunosuppressed patients. tuberculosis rates in the south remain above the national average. physicians diagnosing tuberculosis may also treat the disease, working with health departments, which can assist with drugs, follow-up tests, and contact investigation. powerful short-course regimens have been sta ... | 1992 | 1604386 |
| primary tuberculosis of the glans penis. | | 1992 | 1607396 |
| secreted antigens of mycobacterium tuberculosis: characterization with t lymphocytes from patients and contacts after two-dimensional separation. | little is known about t cell antigens involved in immunity against mycobacterium tuberculosis. most model systems use in vitro culture of human t lymphocytes with bacterial lysates or secreted proteins as antigens. in this study, proteins from 3-week-old m. tuberculosis culture filtrates were separated by two-dimensional page and subsequently transferred into soluble phase. the resulting 480 fractions were screened with t lymphocytes from tuberculosis patients and healthy contacts. t cells from ... | 1992 | 1607694 |
| tuberculin skin test reactivity among adults infected with human immunodeficiency virus. | infection with the human immunodeficiency virus type 1 (hiv-1) results in decreased cell-mediated immunity, which includes decreased delayed hypersensitivity to skin test antigens. hiv-1 seropositivity and skin test reactivity to purified protein derivative (ppd) were determined among 2042 healthy haitian adults with normal chest radiographs. among hiv-1-seropositive individuals, 52.3% (146/279) had ppd reactions greater than or equal to 10 mm compared with 67.2% (1184/1763) of the seronegative ... | 1992 | 1607696 |
| usefulness of skin testing with mycobacterial antigens in children with cervical lymphadenopathy. | one hundred twenty-three children with chronic cervical lymphadenopathy were skin-tested with purified protein derivative (ppd)-b (mycobacterium intracellulare), ppd-y (mycobacterium kansasii), ppd-g (mycobacterium scrofulaceum) (nontuberculous mycobacterial antigens (ntmags)) and ppd-t (mycobacterium tuberculosis). children with culture-confirmed mycobacterial disease had significantly larger reactions to ntmags and were 6 times more likely to have ppd-b responses of greater than or equal to 10 ... | 1992 | 1608681 |
| in vitro properties of the newer quinolones. | table 5 summarizes the activity of the newer quinolones against various bacteria including intracellular bacteria and the other microorganisms. in this table, the overall mic ranges of nflx, enx, oflx, and cpfx, for susceptible isolates of each bacteria are schematically presented. the newer quinolones are considered to have sufficient activity against gram-negative enteric bacteria, n. gonorrhoeae, and h. influenzae and legionella spp. since oflx and cpfx show only moderate activity against sta ... | 1992 | 1609115 |
| human immunodeficiency virus and infected aneurysm of the abdominal aorta: report of three cases. | three patients who were seropositive for human immunodeficiency virus underwent surgery for infected aneurysm of the abdominal aorta. fever and abdominal pain were the principal presenting clinical features. none of the patients had any opportunistic infections or endocarditis. in two cases, a ruptured aneurysm was demonstrated radiographically. in the remaining case, sonograms were diagnostic. the organisms responsible were salmonella, hemophilus influenzae, and mycobacterium tuberculosis. in t ... | 1992 | 1610655 |
| phosphate starvation enhances expression of the immunodominant 38-kilodalton protein antigen of mycobacterium tuberculosis: demonstration by immunogold electron microscopy. | in this work, we grew mycobacterium tuberculosis in an enriched proskauer-beck-youmans culture medium in the presence and in the absence of phosphate salts. immunoblot analysis of sonic extracts showed overexpression of the 38-kda protein antigen by bacilli grown in the medium without phosphate. these observations were confirmed by immunogold electron microscopy, which showed that the number of gold particles was significantly higher in bacilli grown in medium without phosphate than in bacilli g ... | 1992 | 1612766 |
| adjuvant composition determines the induction of type ii collagen-induced arthritis. | in this study we have investigated the influence of adjuvant composition on the development of collagen-induced arthritis and of anti-collagen type ii specific b- and t-cell responses following immunization with type ii collagen. dba/l mice immunized with bovine collagen type ii emulsified in complete freund's adjuvant (cfa) containing mycobacterium tuberculosis strain h37ra developed footpad swelling indicative of arthritis. animals immunized with collagen type ii plus cfa containing mycobacter ... | 1992 | 1615283 |
| an outbreak of tuberculosis caused by multiple-drug-resistant tubercle bacilli among patients with hiv infection. | to evaluate a nosocomial outbreak of tuberculosis caused by multiple-drug-resistant bacilli among patients with tuberculosis and hiv infection. | 1992 | 1616211 |
| clinical presentation and outcome of patients with hiv infection and tuberculosis caused by multiple-drug-resistant bacilli. | to determine the clinical manifestations of patients with human immunodeficiency virus (hiv) infection and tuberculosis caused by multiple-drug-resistant bacilli compared with those with single-drug-resistant or susceptible bacilli. | 1992 | 1616212 |
| cutaneous tuberculosis: a rare presentation of malignancy. | an 88-year-old woman presented with fever, a neck ulcer, and multiple subcutaneous nodules on her upper extremities and thorax. her condition was initially diagnosed as malignancy associated with metastatic disease to the skin. a subcutaneous nodule was aspirated. a gram stain of the aspirate revealed weakly gram-positive bacilli, and a stain for acid-fast bacilli was positive. mycobacterium tuberculosis was isolated from a culture of a specimen of the skin lesion. | 1992 | 1617055 |
| heat shock proteins and arthritis. | | 1992 | 1617333 |
| tuberculous abscess of the pancreas. case report and review of the literature. | | 1992 | 1618064 |
| high risk of active tuberculosis in hiv-infected drug users with cutaneous anergy. | to determine the incidence of active tuberculosis in human immunodeficiency virus (hiv)-seropositive and hiv-seronegative drug injectors with cutaneous anergy and to examine the effectiveness of isoniazid chemoprophylaxis in preventing tuberculosis among drug injectors with positive tuberculin test results. | 1992 | 1619742 |
| risk stratification for dissemination of tuberculosis in hiv-infected patients. | the location of tuberculosis (tb) early in the course of hiv-induced immunosuppression was located, and an attempt was made to determine the correlation between the degree of immune suppression and prognostic variables to stratify the risk for dissemination of tb in hiv-infected patients. clinical and laboratory characteristics were reviewed in 73 hiv-infected patients with tb admitted between 1984 and 1990. the presence of mycobacterium tuberculosis was investigated in different clinical sample ... | 1992 | 1620815 |
| [antibodies against mycobacterial antigens in patients with non-tuberculous pathology]. | antibodies (type igg) against the a 60 antigen (mycobacterium bovis, bcg) and an antigen of mycobacterium tuberculosis were studied by elisa in 79 patients with not tuberculous disease: 16 lung carcinoma, 16 hepatic cirrhosis and 47 patients with pulmonary disease. 59.5% and 50.6% of the patients showed antibodies against antigen a 60 and m. tuberculosis antigen respectively; more 25% were strongly positive against antigen a 60; in the patients with lung carcinoma this results were 62.5%. it is ... | 1992 | 1620920 |
| antagonism between isoniazid and the combination pyrazinamide-rifampin against tuberculosis infection in mice. | mice that had been inoculated intravenously with 6.30 log10 mycobacterium tuberculosis h37rv 14 days earlier were administered one of three combinations of drugs, i.e., isoniazid (inh)-rifampin (rmp)-pyrazinamide (pza), inh-rmp, and rmp-pza, during an initial 2-month period to mimic the initial phase of chemotherapy for human tuberculosis and during a later 4-month period to mimic the continuation phase of chemotherapy. at the end of the initial phase, all three combined regimens were found to h ... | 1992 | 1622164 |
| genetic control of immune responses to the 18-kda protein of mycobacterium leprae. different th1 subsets may be involved in proliferative and delayed-type hypersensitivity responses. | in vitro and in vivo responses to the 18-kda protein of mycobacterium leprae have been analysed in different strains of mice. lymphocytes from balb/cj (h-2d), balb.b (h-2b), b10.br (h-2k), and b10.m (h-2f) mice primed with 18-kda protein yielded high t cell proliferative responses, while those from c57bl/10j (h-2b) mice yielded lower responses. both h-2 and non-h-2 genes contributed to the magnitude of responsiveness. f1 mice from high and low responder strains showed high responsiveness to the ... | 1992 | 1623551 |
| the role of bronchoscopy in the diagnosis of pulmonary tuberculosis in patients at risk for hiv infection. | the present study was undertaken to clarify the role of bronchoalveolar lavage (bal) and transbronchial biopsy (tbb) in the diagnosis of pulmonary tuberculosis in patients at risk for human immunodeficiency virus (hiv) infection. we retrospectively identified 31 patients at risk for hiv who proved to have mycobacterium tuberculosis on culture of at least one pulmonary specimen. all had pulmonary symptoms but initial sputum smears negative for acid-fast bacilli (afb). all underwent fiberoptic bro ... | 1992 | 1623742 |
| purification of sputum samples through sucrose improves detection of mycobacterium tuberculosis by polymerase chain reaction. | a method is described for the routine preparation of sputum samples for detection of mycobacterium tuberculosis by using polymerase chain reaction amplification. liquefaction of sputum samples with naoh and subsequent removal of inhibitors of the polymerase reaction with a 50% sucrose centrifugation step (5 min) in a desktop centrifuge allow direct amplification of a 123-bp repetitive region of the m. tuberculosis genome. we have evaluated the sensitivity and specificity of the sucrose method wi ... | 1992 | 1624570 |
| cytokine production induced by mycobacterium tuberculosis lipoarabinomannan. relationship to chemical structure. | lipoarabinomannan (lam), a major cell wall component of mycobacterium tuberculosis, exhibits a wide spectrum of immunoregulatory effects. to identify cytokines produced by human pbmc in response to lam, we used pcr amplification to detect cytokine mrna. lam-induced transcription of mrna for cytokines characteristically produced by macrophages, including tnf, granulocyte-macrophage-csf, il-1 alpha, il-1 beta, il-6, il-8, and il-10. in contrast, lam did not induce transcription of mrna for cytokin ... | 1992 | 1624801 |
| [detection of mycobacteria by polymerase chain reaction]. | the polymerase chain reaction (pcr) was used to detect mycobacterial dna sequences in the cultured or the clinical specimens. four oligonucleotide primers derived from the sequence of a gene coding 65-kilodalton antigen of mycobacterium tuberculosis amplified dna samples of all the 11 species of mycobacteria tested. serial dilution of m. bovis bcg showed that dna extracted from only 12 bacilli was enough for the detection by pcr method. however, mycobacteria in sputum were detected by pcr when m ... | 1992 | 1625404 |
| osteoarticular tuberculosis of the symphysis pubis presenting as a hypogastric cystic mass in a woman with primary sjögren's syndrome. | a 79-year-old woman with primary sjögren's syndrome and immune thrombocytopenia presented with a hypogastric cystic mass. twenty-three months previously she received a 7-month course of prednisone at moderate doses to control the thrombocytopenia. a computed tomography showed a cystic mass, destruction of the left pubis with involvement of the symphysis and erosion of the right pubis. the cystic mass was surgically removed. epithelioid granulomata were found on pathological examination and mycob ... | 1992 | 1628172 |
| [rapid diagnosis of tuberculous meningitis by polymerase chain reaction (pcr)]. | using polymerase chain reaction (pcr), a rapid and highly sensitive method was developed for the diagnosis of tuberculous meningitis. the mycobacterium tuberculosis (m. tuberculosis) genome was detected in csf of 5 of 6 patients with clinically diagnosed tuberculous meningitis, but not present in csf from 10 patients with other types of meningitis and 10 normal controls. furthermore, we detected m. tuberculosis in csf of 2 of 24 patients with clinically possible tuberculous meningitis. this is h ... | 1992 | 1628444 |
| influence of h-2 genes on growth of mycobacterium tuberculosis in the lungs of chronically infected mice. | mice infected by intraperitoneal injection of mycobacterium tuberculosis were studied over a 23-week period. they showed progressive infection in the lung (with increasing microbial count and granuloma size) whereas viable bacillary counts remained largely stationary in the spleen and in the liver. the influence of h-2 genes on the progression of the lung infection was studied in four congenic strains of animals with b10 and three congenic strains of animals with balb backgrounds. h-2k mice had ... | 1992 | 1628890 |
| tubercular colitis in a patient with systemic lupus erythematosus: diagnosis by stool examination. | | 1992 | 1629130 |
| transmission of multidrug-resistant tuberculosis among immunocompromised persons in a correctional system--new york, 1991. | during 1990 and 1991, nosocomial transmission of multidrug-resistant tuberculosis (mdr-tb) was documented in four hospitals in new york and florida. subsequently, additional mdr-tb outbreaks have been investigated by cdc. this report summarizes preliminary results of an investigation of transmission of mdr-tb in a correctional facility in new york. | 1992 | 1630427 |
| [abdominal tuberculosis today. a review of 46 cases]. | the clinical features, radiological and therapeutic response of 46 cases of abdominal tuberculosis (at) seen at a university hospital are presented. diagnosis was anatomopathologic in 39 cases (85%) and clinical with response to tuberculostatic in 7 cases (15%). most of the patients did not have history nor exposition to tuberculosis. both sexes were similar affected, mean age 43 years old, between 11 and 79. clinical manifestations were no specific, the most frequent fever (65%), abdominal pain ... | 1992 | 1631355 |
| [multifocal tubercular osteitis in a female patient infected with the human immunodeficiency virus]. | we discuss the case of a 22-year-old female, carrying of antibodies to against human immunodeficiency virus (hiv), who developed several tumors on different bone localizations, which corresponded to plurifocal bone tuberculosis. we reviewed multifocal bone tuberculosis reports in the literature. the exceptionally of this case in underlined and the relationship between this atypical clinical presentation and immunodeficiency in discussed. | 1992 | 1631360 |
| superinfection of chronic necrotizing pulmonary aspergillosis by mycobacterium tuberculosis. | a 63-year-old woman with chronic necrotizing pulmonary aspergillosis complicated by active pulmonary tuberculosis is reported. a small infiltrative shadow appeared, but no definite diagnosis was made. six years later the shadow was found to have increased in size; chest ct revealed a fungus ball, while a transbronchial lung biopsy revealed aspergillus hyphae. the intrabronchial inoculation of amphotericin b proved ineffective, and a lobectomy was performed. histopathologic findings showed necrot ... | 1992 | 1633366 |
| survey of mycobacteria isolated from urine and the genitourinary tract in south-east england from 1980 to 1989. | between 1980 and 1989, the public health laboratory service regional tuberculosis centre, dulwich, received cultures of mycobacteria isolated from urine and the genitourinary tract of 1392 new patients: 803 isolates were members of the tuberculosis complex (753 m. tuberculosis, 45 m. bovis, 4 m. africanum) and 589 were various species of environmental mycobacteria. the incidence of the latter isolations varied by region and by year and, with 17 exceptions (13 from endometrial curettings, 2 from ... | 1992 | 1638350 |
| monoclonal antibodies specific for elongation factor tu and complete nucleotide sequence of the tuf gene in mycobacterium tuberculosis. | monoclonal antibodies against mycobacterial antigens were produced by immunizing lou/c rats with live mycobacterium bovis bcg. the antibodies were characterized by an enzyme-linked immunosorbent assay and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by western blotting (immunoblotting). one antibody, mamb 2, reactive with a 47-kda protein was used to screen a lambda gt11 m. tuberculosis gene library (r. a. young, b. r. bloom, c. m. grosskinsky, j. ivanji, d. thomas, and ... | 1992 | 1639483 |
| mycobacterium haemophilum causing perihilar or cervical lymphadenitis in healthy children. | we describe five apparently immunologically normal children with infection caused by mycobacterium haemophilum and resulting in perihilar or cervical lymphadenitis. clinical distinction from mycobacterium tuberculosis infection could be made by culture only, because differential mantoux test results suggested tuberculosis. treatment of these children involved chemotherapy or surgery or both, and outcome in all cases has been excellent. we believe that m. haemophilum should be added to the list o ... | 1992 | 1640283 |
| from the centers for disease control. transmission of multidrug-resistant tuberculosis among immunocompromised persons, correctional system--new york, 1991. | | 1992 | 1640597 |
| fatty acid-sensitive acid phosphatase activity of tubercle bacilli. | in a whole cell assay system with p-nitrophenyl phosphate as substrate, strains of mycobacterium tuberculosis and m. bovis were identical in the ph-activity pattern of acid phosphatase. it was a one-peak curve with a ph optimum at 6.2 and sharp symmetrical slopes. the enzymatic activity did not reflect the virulence. when the cells were subjected to mechanical fractionation, the major part of the enzymatic activity was found in a particulate fraction and a minor portion in supernatant and cell w ... | 1992 | 1640647 |
| national action plan to combat multidrug-resistant tuberculosis. | at no time in recent history has tuberculosis (tb) been as great a concern as it is today. tb cases are on the increase, and the most serious aspect of the problem is the recent occurrence of outbreaks of multidrug-resistant (mdr) tb, which pose an urgent public health problem and require rapid intervention. a task force composed of representatives of many federal agencies has developed a national action plan for addressing this problem. the task force identified a number of objectives to be met ... | 1992 | 1640920 |
| management of persons exposed to multidrug-resistant tuberculosis. | recent outbreaks of multidrug-resistant tuberculosis (mdr-tb) have posed challenges for the management of exposed persons. this report offers suggestions for evaluating and managing persons (i.e., contacts) who have been exposed to patients with infectious mdr-tb (tb due to strains of mycobacterium tuberculosis resistant to both isoniazid [inh] and rifampin [rif]), provides background information on alternative preventive therapy regimens with drugs other than inh or rif, and presents considerat ... | 1992 | 1640921 |
| the increasing prevalence of resistance to antituberculosis chemotherapeutic agents: implications for global tuberculosis control. | | 1992 | 1642799 |
| susceptibility of strains of the mycobacterium tuberculosis complex to fusidic acid. | the activity of fusidic acid was studied in 40 strains of m. tuberculosis (of which 20 strains were mono- or multiresistant to standard antituberculosis drugs) and 10 strains of m. bovis. minimum inhibitory concentration (mic) was determined by the radiometric (bactec) broth method. the mic for the 50 strains varied between 8 and 32 mg/l, with a mic90 of 16 mg/l for m. tuberculosis and a mic90 of 32 mg/l for m. bovis. minimal bactericidal concentration (mbc, defined as the lowest concentration o ... | 1992 | 1642855 |
| a comparative serological study of antigenic glycolipids from mycobacterium tuberculosis. | two fractions of antigenic diacyl trehaloses (dat1 and dat2) were isolated from the type strain of mycobacterium tuberculosis (h37rv). phenolic glycolipid (pgl) and polar glycolipid antigens (c1-c4) were isolated from an unusual smooth so-called 'canetti' strain of m. tuberculosis. these lipids were analyzed by enzyme-linked immunosorbent assay (elisa) using antisera against a range of mycobacteria and sera from 50 tuberculosis patients and 25 healthy blood donors. all the lipids gave strong rea ... | 1992 | 1643294 |
| primary and acquired drug resistance in adult black patients with tuberculosis in south africa: results of a continuous national drug resistance surveillance programme involvement. | drug resistance is a major problem in the treatment of tuberculosis, and monitoring programmes are essential in control of this disease. the extent of primary resistance in a community is an important indication of the effectiveness of treatment schedules. since 1965 the tuberculosis research institute (tbri) of the south african medical research council has performed 25 annual surveys of drug resistance in adult black tuberculosis patients where the disease is most prevalent. methodology for pa ... | 1992 | 1643295 |
| high rate of rifampicin resistance of mycobacterium tuberculosis in the taif region of saudi arabia. | this retrospective study was undertaken to determine the prevalence and pattern of resistance to antituberculosis drugs among patients with sputum-proven pulmonary tuberculosis who were seen in taif chest hospital over 24 months (between june 1986 and may 1988). the overall prevalence was 22.6% and the majority (53%) were resistant to two drugs. resistance to streptomycin was most frequent (16%) followed by rifampicin (15%). resistance to isoniazid was surprisingly low (6.5%). 23.3% of the resis ... | 1992 | 1643296 |
| activity of two long-acting rifamycins, rifapentine and fce 22807, in experimental murine tuberculosis. | the efficacy of the long-acting rifamycins, rifapentine (rpe) and fce 22807 (fce) in experimental murine tuberculosis was studied by counting viable bacilli in spleens. at 2 weeks after infection with mycobacterium tuberculosis, strain h37rv, treatment with isoniazid 25 mg/kg, rifampicin 10 mg/kg and pyrazinamide 150 mg/kg was given daily for 6 weeks. the mice were then divided into groups given rpe or fce at intervals of 1, 2 or 3 weeks with spleen counts after 18 and 24 weeks of chemotherapy. ... | 1992 | 1643297 |
| patterns of contrast enhancement of tuberculous lymph nodes demonstrated by computed tomography. | the purpose of this study was to evaluate the contrast-enhanced ct characteristics of pathologic thoraco-abdominal lymph nodes in 38 patients infected with mycobacterium tuberculosis (30 without aids and eight with aids). unenhanced scans of chest, abdomen and pelvis were also done in all cases. the ct attenuation values and the enhancement characteristics of the largest node or group of lymph nodes were evaluated in a dynamic sequence over a period of 10 min. on unenhanced ct the nodes were of ... | 1992 | 1643775 |
| tuberculosis in patients with human immunodeficiency virus infection. how often does it mimic pneumocystis carinii pneumonia? | adjunctive corticosteroid therapy is recommended for selected human immunodeficiency virus (hiv)-infected patients with presumed pneumocystis carinii pneumonia. because corticosteroids may exacerbate undiagnosed tuberculosis, we evaluated the frequency with which tuberculosis in hiv-infected patients mimics p carinii pneumonia. over a 12-month period, we identified 105 hiv-infected patients with pleuropulmonary tuberculosis and 84 patients with p carinii pneumonia who were sufficiently hypoxemic ... | 1992 | 1643927 |
| [mycobacteria tuberculosis bacteremia]. | mycobacterial bacteremia is common in patients with acquired immunodeficiency syndrome, and mycobacterium avium-intracellulare is isolated most frequently. although bacteremia due to mycobacterium tuberculosis was described as early as 1917, there are few recent reports regarding this phenomenon. however, mycobacterium tuberculosis infection is relative rare in those developed countries as compared to ours. it is estimated about 12,000 newly diagnosed tuberculous patients in taiwan area every ye ... | 1991 | 1646680 |
| monocyte responses to sulfatide from mycobacterium tuberculosis: inhibition of priming for enhanced release of superoxide, associated with increased secretion of interleukin-1 and tumor necrosis factor alpha, and altered protein phosphorylation. | in monocytes, sulfatide, a lipid from mycobacterium tuberculosis, blocked priming for enhanced release of superoxide (o2-) by the macrophage activating factors lipopolysaccharide, gamma interferon, interleukin-1 beta (il-1 beta), tumor necrosis factor alpha (tnf-alpha), and muramyl dipeptide. sulfatide, in the presence of lipopolysaccharide, also caused increased secretion of il-1 beta and tnf-alpha into monocyte culture medium. sulfatide altered the pattern of phosphorylation of monocyte protei ... | 1991 | 1649796 |
| insertion element is987 from mycobacterium bovis bcg is located in a hot-spot integration region for insertion elements in mycobacterium tuberculosis complex strains. | most strains of the mycobacterium tuberculosis complex carry multiple copies of an is3-like element, and these strains are highly polymorphic with regard to the site of integration in the chromosome. in contrast, mycobacterium bovis bcg contains a single copy of the insertion element, and in all strains this copy is integrated at the same site in the chromosome. in this study, we determined the sequence of the single-copy insertion element from m. bovis bcg, is987, and its flanking regions. the ... | 1991 | 1649798 |
| increase of t-cell receptor gamma/delta-bearing t cells in cord blood of newborn babies obtained by in vitro stimulation with mycobacterial cord factor. | cord blood t lymphocytes proliferated in vitro in response to mycobacterial organisms but did not proliferate in the presence of tuberculin purified protein derivative. components recognized by cord blood t cells were resistant to protease digestion. in contrast, t lymphocytes derived from tuberculin-positive adult peripheral blood proliferated when stimulated by the protease-sensitive component of mycobacterial organisms or purified protein derivative, confirming that adult t cells respond to p ... | 1991 | 1652559 |
| detection of mannophosphoinositide antigens in sputum of tuberculosis patients by dot enzyme immunoassay. | a simple and economical dot elisa for the detection of mannophosphoinositide antigen in sputum samples of tuberculosis patients has been developed using affinity-purified antibodies. this test is able to detect free as well as bound antigen. sputum samples from 94 patients suffering from tuberculosis and 30 non-tuberculosis patients were screened and an overall sensitivity and specificity of 89% and 93.3%, respectively, was obtained. the sensitivity of the test among the different groups of tube ... | 1991 | 1652678 |
| fiberoptic bronchoscopic diagnosis of pulmonary disease in 151 hiv-infected patients with pneumonitis. | in a prospective study the efficacy of fiberoptic bronchoscopy was evaluated in the diagnosis of infections with opportunistic pathogens, kaposi's sarcoma and nonspecific interstitial pneumonitis in 171 episodes of pneumonitis in 151 hiv-infected patients. samples were collected by suction through the inner aspiration channel of the bronchoscope (n = 164), telescoping plugged catheter (n = 117) and transbronchial lung biopsy (n = 82). a high incidence of infections with pyogenic bacteria (12%), ... | 1991 | 1655432 |
| tuberculosis takes a turn for the worse. | | 1991 | 1655874 |
| [comparison of bactec radiometric system and lowenstein-jensen medium for detection of mycobacterium tuberculosis and the susceptibility of isolates to antituberculosis drugs]. | tuberculosis is a chronic, durable but curable disease. active cases are usually contagious and spread easily to the close intimates, especially in children causing catastrophic complications. thereafter, early diagnosis and adequate treatment is critical for the control of the disease. bactec 460 12b medium (bactec system) provides a rapid, accurate and reliable method for the detection and differentiation of mycobacterium tuberculosis. it is better than the traditional method, lowenstein-jense ... | 1991 | 1657338 |
| the t cell receptors of human gamma delta t cells reactive to mycobacterium tuberculosis are encoded by specific v genes but diverse v-j junctions. | the observation that gamma delta t lymphocytes react to mycobacteria has provided an important model for investigation of these cells in the immune response to infection. one important question regarding human gamma delta t cells is the breadth of the t cell repertoire in response to specific pathogens. the present study was undertaken to characterize, in molecular terms, the mycobacterium-specific gamma delta tcr repertoire. mononuclear cells were isolated from the peripheral blood and pleural ... | 1991 | 1658146 |
| a predominance of the t cell receptor v gamma 2/v delta 2 subset in human mycobacteria-responsive t cells suggests germline gene encoded recognition. | little is known about the nature of ag recognition by the tcr-gamma delta. the recent observation that gamma delta t cells preferentially recognize mycobacterial ag provides a model to examine the molecular basis of gamma delta-tcr recognition. here, examination of the mycobacteria-stimulated peripheral blood t cells with tcr-specific mab revealed a predominance of t cells bearing v gamma 2/v delta 2 gene products. pcr cloning and sequence analysis of the tcr chains demonstrated extensive juncti ... | 1991 | 1658147 |
| [antimycobacterial activities of a new quinolone, sparfloxacin]. | sparfloxacin (spfx), a new quinolone, was studied its in vitro and in vivo activities against various mycobacteria, especially mycobacterium intracellulare. spfx exhibited a potent in vitro activity against m. tuberculosis, m.kansasii and m.fortuitum with mic90 values of 0.2, 6.25 and 1.6 micrograms/ml, respectively, and the potency of spfx was higher than that of ofloxacin (oflx). m.marinum, m.scrofulaceum, m.avium, m.intracellulare, m.chelonae (subsp. abscessus and chelonae) were resistant to ... | 1991 | 1660089 |
| in vitro and in vivo activities of sparfloxacin (at-4140) against mycobacterium tuberculosis. | the in vitro and in vivo activities of sparfloxacin (at-4140) against m. tuberculosis are reported. the mics of sparfloxacin for 50% and 90% of 18 clinical isolates were, respectively, 0.25 and 0.5 mg/l, one or two dilutions lower than that of ciprofloxacin and ofloxacin. in mice infected intravenously with 0.1 mg m. tuberculosis h37rv strain, the minimal effective dosage of sparfloxacin, as assessed by survival rate, spleen enlargement and gross lung lesions, was 12.5 mg/kg. the activities of v ... | 1991 | 1663283 |
| chromosomal dna fingerprint patterns produced with is6110 as strain-specific markers for epidemiologic study of tuberculosis. | mycobacterium tuberculosis isolates were studied by comparing chromosomal dna fingerprint patterns produced by digestion of chromosomal dna with bamhi, followed by agarose electrophoresis and hybridization with radiolabeled probes of insertion sequence is6110. dna fingerprints of 14 isolates from separate members of five households or closely associated individuals were compared. marked differences were observed when unrelated isolates were compared. there were no or minimal differences in the r ... | 1991 | 1663520 |
| identification of an insertion sequence, is1081, in mycobacterium bovis. | an insertion sequence, is1081, in the genome of mycobacterium bovis has been identified and sequenced. it is 1324 bp long with 15 bp inverted repeat ends and contains a large orf. there are six copies of is1081 in the genome of m. bovis and the element is also present in mycobacterium tuberculosis. is1081 is not closely related to other dna elements described in actinomycetes but its putative transposase bears some resemblance to that of is256 from staphylococcus aureus. is1081 may be useful for ... | 1991 | 1663885 |
| comparison of dna fragment patterns between the phenolic glycolipid-tb producers and non-producers of mycobacterium tuberculosis. | differences in ability to produce the specific phenolic glycolipid-tb (pgl-tb) antigen among mycobacterium tuberculosis strains have been reported. one of the explanations would be the genotypic variation between the strains. in this study, we compared the dna fragment patterns after digestion of dna with various restriction enzymes between the pgl-tb producing and non-producing strains of m. tuberculosis. three clinical isolates of m. tuberculosis producing the pgl-tb antigen detectable by thin ... | 1991 | 1664164 |
| calmodulin-like activity in mycobacteria. | calmodulin-like activity has been reported for the first time in mycobacterial species, namely mycobacterium tuberculosis bcg and m. smegmatis atcc 14468. the activity was mainly located in the soluble fraction of the mycobacterial cells, radioimmunoassay revealed maximum levels of calmodulin in young growing cells (early logarithmic phase of growth). calmodulin-dependent phosphodiesterase activation assay revealed low activity (22%) of partially purified calmodulin either due to insufficient am ... | 1991 | 1667396 |
| guinea-pig alveolar macrophage killing of mycobacterium tuberculosis, in vitro, does not require hydrogen peroxide or hydroxyl radical. | alveolar macrophages from the lungs of guinea-pigs that had been vaccinated, boosted and then intravenously challenged with mycobacterium bovis bcg, killed both a hydrogen-peroxide-resistant and a hydrogen-peroxide-sensitive strain of m. tuberculosis, in vitro. pretreatment of the alveolar macrophages, in vitro, with catalase or mannitol, agents which remove hydrogen peroxide and the hydroxyl radical, respectively, did not decrease the extent of killing of either strain. in contrast, pretreatmen ... | 1991 | 1667538 |
| enhancement of monocyte antimycobacterial activity by diethyldithiocarbamate (dtc). | diethyldithiocarbamate (dtc) has been recently reported to significantly reduce the incidence of opportunistic infections in hiv-infected patients. the present study addresses the question whether dtc is capable of stimulating antimycobacterial activity of mononuclear phagocytes. we found that peripheral blood mononuclear cells (pbmc) of healthy subjects preincubated in vitro with 100-1000 ng/ml of dtc and thereafter infected with mycobacterium tuberculosis h37rv or mycobacterium avium-intracell ... | 1991 | 1667649 |
| [pulmonary tuberculosis today. the findings over a 5-year period in a ward for women]. | the authors studied the cases of pulmonary tuberculosis they had the opportunity to observe during a five-year period from 1985 to 1989 carrying out the phenomenon. as a result they pointed out that the worst affected subjects belonged to the age bands included between the second and the third decade, followed by the fifth decade and over. they concluded that the 234 cases they examined do not represent the real number of affected subjects and that elderly patients to the most serious cases. | 1990 | 1669275 |
| [pulmonary tuberculosis in an immigrant population: the epidemiological aspects and clinical picture]. | the number of extra european subjects coming to our country, in the last twenty years, is increased. this fact has contributed to enlarge the number of new cases of tuberculosis. in the present work we analyzed 54 extra european and 116 italian patients with pulmonary tuberculosis observed in the last six years in our institute. the diagnosis was established by finding acid-fast bacilli in sputum and by clinical and radiographic patterns. we found that extra european subjects show disseminated a ... | 1990 | 1669276 |
| a study of different parameters of cell-mediated immunity in patients with active and chronic tuberculosis. | a study was carried out on cell-mediated immunity in healthy persons and patients with tuberculosis in order to extend the diagnostic capacity in tuberculosis. we studied the relation between baciloscopy and the state of specific cell-mediated immunity in vivo (mantoux) and in vitro (leukocyte migration inhibition assay with ppd-rt 23 to evaluate specific cell-mediated immunity and pha to evaluate nonspecific immunity) in 131 patients with active tuberculosis, in 63 patients with chronic tubercu ... | 1991 | 1669579 |
| cellular and humoral reactivity pattern to the mycobacterial heat shock protein hsp65 in adjuvant arthritis susceptible and resistant wistar rats. | we have analyzed the cellular and humoral immunity to the mycobacterial 65 kda heat shock protein (hsp65) in a group of freund's adjuvant-immunized rats with a limited susceptibility to adjuvant arthritis. according to the arthritis indices during the period of study (35 days), two different groups of rats could be distinguished; a) autoimmune adjuvant arthritic rats (aa), and b) non-arthritic animals (na), including both rats which did not display any disease symptoms and rats suffering mild tr ... | 1991 | 1669842 |
| the pathology of severe morbidity and mortality caused by hiv infection in africa. | aids is the leading cause of death among adults in at least two capital cities in africa: abidjan, cote d'ivoire, and kinshasa, zaire. knowledge of the causes of morbidity and mortality in hiv-positive people in africa is, however, less than complete due to limited postmortem diagnostic facilities, difficulties in obtaining consent for postmortem examinations, and a general lack of pathologists. although hiv affects all organs, either directly or by facilitating opportunistic infections and tu ... | 1991 | 1669911 |
| [description of 2 new enzymatic bio-varieties of mycobacterium tuberculosis]. | biovarieties of m. tuberculosis were studied in 736 isolates. most of them were classified into the well known varieties but two were considered as new varieties nominated h and i. variety h has valine and cystine aminopeptidases, is active also against trypine, and contains alpha glucosides. variety i contains the enzymes plus alfa glucosidase. | 1991 | 1670259 |
| [tuberculosis in the cervical spine of a young woman]. | | 1991 | 1670514 |