[effect of free fatty acids on propionibacterium acnes and staphylococcus epidermidis]. | | 1986 | 3795558 |
histamine production by propionibacterium acnes in batch and continuous culture. | histamine production by propionibacterium acnes was studied using batch and continuous culture. in batch culture, the culture ph and the production of histamine closely paralleled the pattern of growth, increasing as the growth increased. continuous culture studies using a chemostat and varying culture ph showed that highest levels of growth of p. acnes occurred at ph 6.0 and that two clear 'peaks' of histamine production were apparent at ph 4.5 and at ph 7.5. the levels measured at ph 7.5 were ... | 1986 | 3796303 |
canine oral melanoma: comparison of surgery versus surgery plus corynebacterium parvum. | eighty-nine dogs with malignant oral melanoma were selected for study. all dogs were clinically staged and treated with either surgical excision alone or surgery plus c. parvum immunotherapy. there was no difference in survival time between the two treatment groups. however, in dogs with advanced disease (stages ii, iii) there was a statistical difference between surgery alone versus surgery plus c. parvum (p = 0.01). dogs with stage i disease (tumor less than 2 cm diameter) had a statistically ... | 1986 | 3801954 |
pharmacological analysis of guinea-pig macrophage chemiluminescence responses to platelet activating factor and opsonized zymosan. | the luminol-dependent chemiluminescence (cl) response in vitro of guinea-pig c. parvum-activated peritoneal macrophages to platelet activating factor (paf) has been compared with that to opsonized zymosan (opz). the response to paf (5 x 10(-6) mol/l.) reached a peak within 1 min, that to opz (0.17 mg/ml) within 10-20 min. peak responses to both stimuli were dose-dependently inhibited in a similar manner by p-hydroxymercuribenzoate (10(-5) - 10(-3) mol/l), sodium benzoate (10(-5) - 10(-3) mol/l.) ... | 1986 | 3804536 |
distribution of metronidazole susceptibility factors in obligate anaerobes. | the distribution of metronidazole susceptibility factors in clinically important bacteria was studied to determine why obligate anaerobes were susceptible to metronidazole and facultative ones were not. with obligate and facultative anaerobes, nitroreductase activity, metronidazole uptake, pyruvate:ferredoxin oxidoreductase activity and drug susceptibility were estimated. nitroreductase activity was distributed in the obligate anaerobes and some facultative ones such as streptococcus faecalis. p ... | 1986 | 3804881 |
activated macrophage hybridomas secreting a cytotoxic factor. | stable activated macrophage hybridomas were generated by somatic cell fusion between propionibacterium acnes-induced peritoneal exudate cells and ns-1 myeloma cells. five cell lines were obtained and each was cloned by limiting dilution; 59 clones were obtained. the cells of 2 clones (mp4-4 and mp4-8) which adhered to the culture dishes were selected for further analysis. these hybridomas exhibited non-specific esterase and beta-galactosidase intracellularly, and asialo gm1, mac-1, ia antigens a ... | 1986 | 3805745 |
local interferon induction by bacterial lipopolysaccharide in mice after pretreatment with corynebacterium parvum. | c57bl/6 mice were injected intraperitoneally (i.p.) with corynebacterium parvum and subsequently, after an interval of 7-10 days, i.p. with lipopolysaccharide (lps). the peritoneal wash-fluid was recovered at various times after injection of lps. marked interferon (ifn) titers were observed between 2 and 10 h after injection of lps, whereas no ifn was detected in mice injected with either c. parvum or lps alone. very low doses of lps (0.1 microgram/mouse) were sufficient to cause ifn production ... | 1986 | 3805784 |
superiority of intravesical immunotherapy with corynebacterium parvum and allium sativum in control of murine bladder cancer. | intravesical immunotherapy with bacillus calmette-guerin (bcg), corynebacterium parvum (cp), keyhole limpet hemocyanin (klh), and an extract of allium sativum (as) was studied in mice transplanted intravesically with mouse bladder tumor cells (mbt-2). female c3h/he mice were anesthetized with sodium pentobarbital. two x 10(5) mbt-2 cells were delivered into the bladder transurethrally using a small catheter, immediately after the posterior wall of the bladder had been electrocauterized. bladder ... | 1987 | 3806842 |
[osteitis due to anaerobic corynebacteria in children. apropos of 4 cases]. | the authors have seen four instances in children of osteitis due to a mycobacterium. the disease was subacute and situated in the tibia, the lumbar spine, the talus and the femur. in two cases the diagnosis was eventually mistakenly oriented towards tuberculosis or a malignant tumour. the correct diagnosis was obtained after a bone biopsy which demonstrated a granulomatous osteitis. in such cases there is an indication for a full biopsy and bacteriological examination with culture in aerobic and ... | 1986 | 3809649 |
a 31p-nmr study of propionibacterium acnes, including effects caused by near-ultraviolet irradiation. | 161.8 mhz 31p-nmr spectra were recorded from the light sensitive skin bacterium propionibacterium acnes. the cells were grown anaerobically on synthetic phosphate-buffered eagle's medium or on a complex yeast extract medium. the spectra showed a large accumulation of polyphosphates when grown on eagles medium. a splitting of the inorganic phosphate peak indicated a difference between internal and external ph of the cells. addition of glucose to the cell suspension gave rise to a change in the ph ... | 1987 | 3814624 |
head-space gas-liquid chromatographic analysis for presumptive identification of bacteria in blood cultures. | a total of 445 strains of common aerobic and anaerobic bacteria were analyzed for their volatile microbial metabolites by head-space gas-liquid chromatography. the bacteria were incubated for 24 or 48 h in blood-containing trypticase soy or lombard-dowell glucose broth before being analyzed by automatic head-space injection. from the chromatographic pattern obtained, the majority of klebsiella pneumoniae, enterobacter cloacae, serratia marcescens, escherichia coli, pseudomonas aeruginosa, morgan ... | 1986 | 3816354 |
two new vitamin b-12 factors from sewage sludge containing 2-methylsulfinyladenine or 2-methylsulfonyladenine as base component. | two hitherto unknown vitamin b-12 factors were isolated from sewage sludge. they were degraded with cerous hydroxide to cobinamide and the corresponding nucleoside. the nucleosides were further split with dilute hydrochloric acid to the bases and d-ribose. the structure of the two bases was found to be 2-methylsulfinyladenine and 2-methylsulfonyladenine. this was revealed by mass, infrared and nuclear magnetic resonance spectroscopy, and by comparison with the synthetic compounds. on addition of ... | 1987 | 3816796 |
proliferative glomerulonephritis and chronic active hepatitis with cirrhosis associated with corynebacterium parvum immunotherapy in a dog. | | 1987 | 3818425 |
propionibacterium acnes osteomyelitis: case report and review of the literature. | propionibacterium acnes, a part of the skin flora, caused vertebral osteomyelitis in a man who had recently undergone a microdiskectomy. the identifying characteristics and antibiotic susceptibility pattern are presented for this unusual human pathogen. the relationship of p. acnes to bone infection is summarized for the first time. a typical patient with p. acnes osteomyelitis is an adult male who has had prior surgery. most patients respond favorably to prolonged antibiotic therapy. | 1987 | 3818921 |
response of bacterial antigen in palmoplantar pustulosis. | motilities of leukocytes in response to bacterial antigens or sera were examined in tissues from patients with palmoplantar pustulosis (ppp). accelerated migration towards bacterial antigens was detected in the case of staphylococcus epidermidis in 9 of 19 patients, to propionibacterium acnes in 5 of 19, to proteus mirabilis and staphylococcus aureus in 3 of 19, while no acceleration was found in neutrophils from the controls. a significantly accelerated migration of normal lymphocytes in respon ... | 1986 | 3820277 |
[effect of cobalamin derivatives on dna synthesis in cells of propionibacterium freudenreichii subsp. shermanii]. | the increase of dna-synthesis rate (according incorporation [8-14c]adenine) in b12-deficient cells propionibacterium shermanii as a result of different cobalamines adding into the cell suspension including metoxyethyladenile analog of adenozilcobalamin and some components of vitamin b12 molecule has been found. the dna-synthesis rate in b12-deficient cells is nearly twice lower as compared with one in b12-normal cells. considerable stimulative effect (80-100%) was provided with coenzyme forms of ... | 1987 | 3828409 |
induction of the plateau phase and rescue of mopc 104e by nonspecific immunization. | the enhancement of mopc 104e myeloma growth by nonspecific immunostimulation with c. parvum or immunization with sheep red blood cells and levan mixture was evaluated in balb/c mice. we observed increased levels of serum mopc 104e igm and early mortality in the tumor-bearing mice following treatment with c. parvum or antigenic stimulation. the enhancement of tumor growth observed was due to the production of lymphokine mitogenic factors produced in response to the nonspecific immunostimulation. | 1986 | 3828861 |
[a case of infectious propionibacterium acnes endocarditis]. | | 1985 | 3830541 |
[clinical experience with aztreonam in the field of obstetrics and gynecology]. | aztreoman (sq 26,776, azt), a synthetic monobactam antibiotic, was applied clinically in the field of obstetrics and gynecology. azt was administered by intravenous drip infusion for 6 to 8 days at a daily dose of 2 g divided in 2 times to 5 cases. klebsiella in 1 case with puerperal endometritis, enterococcus, propionibacterium and bacteroides in each 1 case with pyometra was isolated. the clinical effect of klebsiella was excellent. bacteroides in 1 not-examined case was good. enterococcus and ... | 1985 | 3834157 |
immunogenicity of corynebacterium pseudotuberculosis and the effect of adjuvants in mice. | intraperitoneal inoculation of cf1 mice with 100 micrograms, 200 micrograms, or 300 micrograms whole cell (wc) or 250 micrograms, 500 micrograms, or 1000 micrograms cell wall (cw) of corynebacterium pseudotuberculosis induced varying degrees of protection after intravenous challenge of immunity with 7.2 x 10(4) cfu of c. pseudotuberculosis. generally, the degree of protection increased with the dose of wc or cw. however, intraperitoneal inoculation of mice with 100 micrograms, 200 micrograms, or ... | 1985 | 3837788 |
[antitumoral activity of various doses of corynebacterium parvum as a function of the age of mice]. | the anti-tumor activity of corynebacterium parvum against two different tumours, a mammary carcinoma grafted in c3h mice and a lymphosarcoma grafted in xvii mice, was lower in young and old mice, than in adult ones. in young mice (25 days) of both lines, low doses of c. parvum were more efficient than higher doses. in 12-month old xvii mice a higher survival rate was obtained by increasing the dose of c. parvum administered, whereas in old c3h mice, this phenomenon was not observed. sublethal do ... | 1985 | 3840664 |
local immunotherapy with propionibacterium granulosum kp-45 in advanced breast cancer. | 50 patients with advanced metastatic breast cancer were matched into 25 "twin" pairs. in each pair, one "twin" received chemotherapy (fac) and the other received chemoimmunotherapy (fac + intratumorally propionibacterium granulosum kp-45 (pg)). the therapeutic effects of this 2-year follow-up study were carefully documented and analysed. the mean survival time of fac + pg-treated patients was about 17 months as compared to 8.5 months in fac controls. pg-therapy responders showed increasing value ... | 1985 | 3840665 |
a sustained increase of microsomal heme oxygenase activity following treatment of rats with bacillus calmette-guerin and corynebacterium parvum: its possible relation to the decrease of cytochrome p-450 content. | the alterations of various enzymes responsible for drug metabolism and heme metabolism were examined in the liver of female rats treated with bacillus calmette-guerin (bcg) and corynebacterium parvum (cp). hepatic drug metabolizing enzyme activities and microsomal cytochrome p-450 and b5 content were significantly decreased for up to 15 and 10 d by a single i.v. administration of bcg and cp, respectively. in contrast, microsomal heme oxygenase activity was markedly increased after bcg and cp tre ... | 1985 | 3841363 |
development of hyporesponsiveness of natural killer cells to augmentation of activity after multiple treatments with biological response modifiers. | four biological response modifiers (brms), mve-2 (maleic anhydride divinyl ether), corynebacterium parvum (c. parvum), polyiclc (polyinosinic:polycytidylic acid stabilized with poly-l-lysine), and mouse alpha beta-interferon (alpha beta-ifn), were tested to assess whether repeated treatments would repeatedly induce or sustain augmented levels of natural killer (nk) cell activity and/or macrophage (m0)-mediated inhibition of tumor cell growth. in contrast to a significant increase in splenic nk a ... | 1985 | 3845849 |
evaluation of in vivo and in vitro effectivity of immune defense against a spontaneously arising, nonlymphoid rat tumor. i. analysis of natural immune defense. | possible mechanisms of natural defense against a nonimmunogenic, nonlymphoid rat tumor were evaluated in vitro and examined for effectivity in vivo, using bsp6s, the subcutaneously grown transplantation line of a spontaneously arising fibrosarcoma in the bdx rat strain, which is highly susceptible to natural killer (nk) cells and macrophages (mø). the role of nonspecific immune defense in vivo was demonstrated by eliminating nk cells by irradiation and mø by silica treatment. especially after de ... | 1985 | 3847288 |
tumour necrosis factor and the lysosomal enzymes of macrophages or macrophage-like cell line. | the relationship between tumour necrosis factor (tnf) and macrophages or macrophage-like cell line, especially the lysosomal enzymes was investigated. the serum lysosomal enzymes and ldh activities were increased in proportion to the tnf production even in different strains of mice. lysosomal enzymes and tnf activity were released into the supernatant of the culture medium of macrophage-enriched peritoneal exudate cells (pec) or spleen cells derived from propionibacterium acnes-primed mice after ... | 1985 | 3851696 |
natural killer inhibitory substance produced by the peritoneal cells of patients with ovarian cancer. | peritoneal cells obtained from 8 patients with minimal residual ovarian cancer produced a substance during in vitro culture that markedly inhibited the expression of natural killer (nk) cell-mediated lysis. its molecular weight was less than 2,000, the same size as the nk-inhibiting substance (nk-is), a similar nk-suppressive molecule produced by the peritoneal cells of rats. human nk-is suppressed the expression of antibody-dependent cell cytotoxicity as well as nk lysis, but it had no effect o ... | 1985 | 3856049 |
stimulation of proliferation in mixed cultures of mouse tumor cells and nonimmune peritoneal cells. i. occurrence of stimulation and cyclical variation in tumor cell activity. | mixtures of mouse tumor cells and resident peritoneal cells (pc) from nonimmune mice frequently incorporated [methyl-3h]thymidine [( 3h]dthd) in culture to a far greater extent than did either cell population alone. with pc-to-tumor cell ratios of 10:1, stimulation indices of up to 21 were recorded. syngeneic, semisyngeneic, and allogeneic mixtures were active. pc elicited by thioglycollate or proteose-peptone could participate in the reaction. pc from mice injected with corynebacterium parvum a ... | 1985 | 3856066 |
immunomodulation of nk and adcc by corynebacterium parvum in acute myeloid leukaemia patients. | natural killer (nk)- and antibody dependent cellular cytotoxicity assays were performed using cryopreserved effector cells in aml patients receiving i.c. and s.c. injections of corynebacterium parvum. a dose related increase in nk could be demonstrated with peaks in nk at day 1 with full cp dosage and at day 14 with 50% doses. this increase was attributable to the cp vaccine since normal donors receiving tetanus toxoid or pneumococcal polysaccharide and aml patients randomized not to receive cp ... | 1985 | 3857405 |
predominant obligate anaerobes in human carious dentin. | with the adoption of an anaerobic glove box system, we isolated and identified the predominant micro-organisms in lesions of carious dentin from four permanent third molars. the overwhelming majority of the micro-organisms isolated from both shallow and deep layers of carious dentin were obligate anaerobes, suggesting that conditions in the dentin were strictly anaerobic. the predominant obligate anaerobes were gram-positive rods which were identified as members of the following genera: propioni ... | 1985 | 3861648 |
immunologic control of a retrovirus-associated murine adenocarcinoma. vi. augmentation of antibody-dependent killing following quantitative and qualitative changes in host peritoneal cells. | attempts were made to augment the antibody-dependent killing of the ascitic ad755a tumor in vivo to protect c57bl/6j mice against the outgrowth of larger tumor burdens. the lethal dose for this tumor is less than 100 cells, and antibodies contained in a hyperimmune antitumor serum (his) were found to suppress the outgrowth of a maximum of about 5 x 10(5) cells. thioglycollate injected ip increased the number of peritoneal macrophages, potential effectors for antibody-dependent cell-mediated cyto ... | 1985 | 3862902 |
immunologic control of a retrovirus-associated murine adenocarcinoma. viii. corynebacterium parvum-activated natural killer cells as potent antibody-dependent cell-mediated cytotoxicity effectors. | the antibody-dependent lytic activity of corynebacterium parvum-induced peritoneal exudate cells was examined in vitro by utilizing ad755a tumor targets and a homologous anti-ad755a hyperimmune serum. maximum antibody-dependent cell-mediated cytolysis (adcc) of tumor targets was achieved within 4 hours of incubation. adcc activity was found primarily in the plastic nonadherent cell population and was greatly enriched following removal of phagocytic cells by carbonyl iron. phenotypically, the cel ... | 1985 | 3862904 |
reduction of serum interleukin-1-like activity after treatment with dexamethasone. | the ability of steroids to modulate the appearance of interleukin-1(il-1) in vivo was evaluated in a model of endotoxin shock. high levels of il-1 were found in serum from a/j mice which were sensitized with p. acnes and challenged with bacterial lipopolysaccharide (lps). the factor appeared in the serum 2-4 hours after lps challenge and was dependent on the period of p. acnes sensitization and the dose of lps. treating the mice with dexamethasone prior to lps challenge resulted in significantly ... | 1985 | 3871468 |
adoptive immunotherapy of newly induced murine sarcomas. | two newly induced methylcholanthrene sarcomas of c57bl/6 mouse origin were selected for studies of the adoptive immunotherapy of established tumors. the mca 105 and mca 106 tumors, used during their first five transplant generations, possessed weakly immunogenic tumor-associated transplantation antigens as revealed by failure to elicit immunity to reject 10(6) tumor cells by tumor growth and excision. specific immunity to reject a 10(6) tumor cell challenge could be elicited in less than 50% of ... | 1985 | 3872168 |
the effect of treatment with corynebacterium parvum on the development and growth of experimental hematogenic metastases of schwannoma in the rat. | single i.v. administration of corynebacterium parvum 5 days before i.v. injection of 10(6) tissue cultured syngeneic schwannoma cells in lewis rats resulted in extension of survival time (p less than 0.05). there was a significant decrease in metastatic tumor incidence for lung, heart, and kidney and decreased lung tumor growth with approximately 50% of the lung tumor burden of untreated controls (p less than 0.05). rats treated similarly with c. parvum 10 days after tumor cell injection showed ... | 1985 | 3876679 |
[functional roles of macrophages in the immunological response to propionibacterium acnes]. | | 1985 | 3879588 |
[effect of intratumor administration of propionibacterium granulosum in patients with advanced breast carcinoma on the clinical course of the disease and the behavior of certain indices of immune resistance]. | | 1985 | 3880373 |
in vitro generation of procoagulant activity by corynebacterium parvum-stimulated mononuclear leukocytes. | thromboembolic complications are known in cancer patients after i.v. administration of corynebacterium parvum. we examined the ability of this organism to induce production of procoagulant activity by human blood mononuclear leukocytes in vitro. after 4 hours incubation corynebacterium parvum was an effective stimulant for mononuclear leukocytes, behaving in the same way as the typical gram-negative bacterium escherichia coli, whereas mononuclear cells incubated with staphylococcus aureus were n ... | 1985 | 3880801 |
increased sensitivity of corynebacterium parvum-treated mice to toxic effects of indomethacin and lipopolysaccharide. | female balb/c and c3h/hej mice develop increased sensitivity to the toxic effects of indomethacin after injection of nonviable corynebacterium parvum. the increased sensitivity developed within 4 days of intraperitoneal injection of the organisms and started to resolve 14 days after injection. the development of increased sensitivity was dependent on the quantity of organisms injected and the concentration of indomethacin utilized. the effect was not observed when c. parvum-treated animals were ... | 1985 | 3881348 |
limited reliability of the "negative" two-dimensional echocardiogram in the evaluation of infectious vegetative endocarditis: diagnostic and surgical implications. | three patients with vegetative endocarditis involving a native valve, a mechanical prosthesis and a bioprosthetic valve respectively are presented. each underwent emergency surgical exploration and prosthetic valve replacement based on clinical evidence of vegetative endocarditis after an initial delay following a report of a negative two-dimensional echocardiographic study. these cases emphasize the fact that within the clinical setting in which vegetative endocarditis is strongly suspected a n ... | 1985 | 3881454 |
adjuvant corynebacterium parvum immunotherapy for squamous cell epitheliomas of the oral cavity, pharynx, and larynx. | patients with primary squamous cell epitheliomas of the oral cavity, pharynx, and larynx were stratified according to stage and site and randomized to receive either intratumoral immunotherapy with corynebacterium parvum followed 2 weeks later by surgery and postoperative c parvum for 2 years or surgery alone. there were 209 patients entered into the trial and 176 were fully evaluable. all prognostic variables were similar between the two groups. there was no difference in disease-free survival ... | 1985 | 3881630 |
postmortem bacteriology of cadaver tissue donors: an evaluation of blood cultures as an index of tissue sterility. | microbiological cultures were performed on the blood and bone marrow of 239 cadaver bone donors and 58 "beating heart cadaver" organ donors who had been asymptomatic of sepsis. the incidence of positive blood cultures was significantly lower among the "beating heart cadaver" donors (8.6%) as compared to other donors from whom tissues were excised up to 30 hr postmortem (38%). microorganisms were isolated from the bones of 82 of 148 (55.4%) bone donors as well as from 36 of 53 (67.9%) "beating he ... | 1985 | 3888506 |
relationship of hypoglycemia to tumor necrosis factor production and antitumor activity: role of glucose, insulin, and macrophages. | the role of hypoglycemia in tumor necrosis factor (tnf) production was examined. tnf was produced from sera of animals presensitized with reticuloendothelial system stimulants after lipopolysaccharide (lps) challenge. blood glucose was strongly reduced during tnf production. glucose administration to presensitized mice (before lps challenge) caused inhibition of tnf production. exogenous insulin injection inhibited tnf production in a dose-related manner. peritoneal exudate cells (pec) from prop ... | 1985 | 3889459 |
macrophage functions in biozzi mice. | the faster degradation of antigen by macrophages in biozzi low (l) responder mice, compared to biozzi high (h) responder mice, is thought to be responsible for their lower antibody response. we have measured four functions associated with macrophages to see whether macrophages from l mice were generally more active than those from h mice. peritoneal macrophages obtained from normal mice were compared with those from groups of mice given mycobacterium bovis bcg or propionibacterium acnes. cells f ... | 1985 | 3894222 |
analysis of protective mechanisms against infection by serratia marcescens. | the present paper is concerned with an experimental study of effects of some agents on parameters pertinent to host resistance to infection of serratia marcescens (s. marcescens) which was isolated from a patient. the results obtained are the following: in the control mice injected intravenously with s. marcescens, most of the bacteria were trapped in the liver, spleen and lung, the so-called reticuloendothelial system (res), and the number of bacteria decreased gradually with time. in the kidne ... | 1985 | 3894671 |
production of tumor necrosis factor in germfree mice. | | 1985 | 3895238 |
quantitative measurements of humoral immune response in mice to a fanft induced bladder tumor. | using unfixed mouse bladder tumor cells (mbt) as target cells and a modified avidin-biotin-complex (abc) method made it possible to detect a humoral immune response in c3h mice with growing mbt tumors. the rise of the serum levels is significant (p less than 0.005) when compared to control animals and correlates with the tumor size. mice with recurrences after surgical removal of the primary tumor had significantly (p less than 0.05) higher serum values than animals without recurrence. purulent ... | 1985 | 3895707 |
adjuvant therapy of cutaneous malignant melanoma: a critical review. | the emergence of revised definitions for the high-risk patient with cutaneous malignant melanoma prompts us to re-examine the current status of adjuvant therapy in this disease. we wish to address the question, "once a cutaneous melanoma is surgically removed and the patient is currently free of disease but at high risk for metastases, what can be done to prevent recurrence"? | 1985 | 3897817 |
cloning and expression of the 1.3s biotin-containing subunit of transcarboxylase. | we have cloned the gene coding for the 1.3s biotin-containing subunit of transcarboxylase (ec 2.1.3.1) from propionibacterium shermanii. transcarboxylase is a well-characterized enzyme composed of 30 polypeptides of three different types: twelve 1.3s biotinyl subunits, six 5s dimeric outer subunits, and one 12s hexameric central subunit. in propionic acid fermentation, the enzyme catalyzes the transfer of a carboxyl group from methylmalonyl-coa to pyruvate in two partial reactions. the 1.3s subu ... | 1985 | 3898065 |
effects of dilution rate on biomass and extracellular enzyme production by three species of cutaneous propionibacteria grown in continuous culture. | propionibacterium acnes, p. avidum and p. granulosum were grown in continuous culture at a range of dilution rates on a semi-synthetic medium. dilution rates were chosen to allow the bacteria to grow at the same relative growth rates as compared to their respective mumax values. the steady-state levels and production rates of biomass and extracellular enzymes were determined. the lipase and hyaluronate lyase of p. granulosum and the proteolytic activity of p. acnes and p. avidum were growth link ... | 1985 | 3900281 |
evaluation of the rapid-ana system for identification of anaerobic bacteria of veterinary origin. | this study evaluated the ability of the rapid-ana system (innovative diagnostic systems, inc., atlanta, ga.) to accurately identify a spectrum of freshly isolated veterinary anaerobes. a total of 183 isolates were tested and included 7 actinomyces spp., 53 bacteroides spp., 32 clostridium spp., 2 eubacterium spp., 65 fusobacterium spp., 1 peptococcus spp., 22 peptostreptococcus spp., and 1 propionibacterium spp. all isolates were initially identified by conventional biochemical testing and gas-l ... | 1985 | 3905854 |
fluorine nmr studies on stereochemical aspects of reactions catalyzed by transcarboxylase, pyruvate kinase, and enzyme i. | the stereochemistry of the transcarboxylase-catalyzed carboxylation of 3-fluoropyruvate has been studied by using fluorine nmr of unpurified reaction mixtures. when the product 3-fluorooxaloacetate was trapped by using malate dehydrogenase, only the 2r,3r diastereomer of 3-fluoromalate was formed. the fluoromethyl group of fluoropyruvate does not take up deuterium label from the solvent during the reaction. these results confirm and extend those obtained previously by walsh and co-workers [golds ... | 1985 | 3910092 |
cytotoxic lymphocytes in rat tumor in situ: effect of intraperitoneal injections of propionibacterium avidum. | in vitro cytotoxicity against tumor cells of lymphocytes in sc implanted bc47 bladder tumor of aci/n rats with or without propionibacterium avidum (p. avidum) treatment was studied. tumor-associated lymphoid cells (tal) were obtained from tumor tissues by mechanical treatment (ndi fraction) and by enzymatic treatment with dispase i, a proteolytic enzyme (di fraction), followed by passage through glass wool columns to deplete tumor cells. ndi fraction of tal from p. avidum-treated animals showed ... | 1985 | 3918910 |
protective effect of lactobacillus casei on pseudomonas aeruginosa infection in mice. | the protective effect of heat-killed lactobacillus casei yit9018 (lc 9018) against pseudomonas aeruginosa infection in mice was compared with that of corynebacterium parvum. survival of mice after intraperitoneal (i.p.) infection with p. aeruginosa was augmented in mice that had been pretreated i.p. with lc 9018 5 days previously. similar treatment of mice with c. parvum, however, was not effective at all. moreover, mice became more susceptible to infection with p. aeruginosa after such treatmen ... | 1985 | 3921464 |
t cell involvement in production of tumor necrosis factor: reconstitution experiments with nude mice. | in order to investigate the role of t cells in the production of tumor necrosis factor (tnf), a reconstitution experiment was performed with nude mice (balb/c, nu/nu). the results obtained were as follows: 1) the cytotoxic activity of tumor necrosis serum (tns) from balb/c, nu/nu mice treated with propionibacterium acnes-lps was 1/22 of that from balb/c, nu/+ mice. 2) tnf activity increased 14 times in reconstituted nude mice as compared to balb/c, nu/nu mice. 3) the production of the cytotoxic ... | 1985 | 3924713 |
drug influences on rat hepatic macrophage enzyme production and release in vitro. | drug influences on hepatic macrophage enzyme release have been investigated using a rat model of macrophage recruitment and activation. n-acetyl-glucosaminidase (nag), a lysosomal enzyme, and plasminogen activator (pa), a cytosolic enzyme, have been measured in both cell lysates and supernatants after 24 h in culture. 6-mercaptopurine (6-mp) and azathioprine significantly decreased (p less than 0.03) the enhanced production of nag by recruited macrophages following stimulation in vitro (total na ... | 1985 | 3926509 |
[effect of c. parvum-activated macrophages on tumor cells: a microcinematographic study]. | | 1985 | 3928111 |
decreased production of interferon alpha and interferon gamma in leucocyte cultures of schizophrenic patients. | in a first approach to measure the activity of the interferon system in schizophrenic patients, leucocyte cultures of schizophrenic patients and normal control individuals were set up using a whole blood assay. in this system both lymphoproliferation and the induction of interferon was tested. the lymphoproliferation (lp) test was performed with one bacterial recall antigen (ppd) and four different mitogens (phytohemagglutinin (pha), concanavalin a (con a), pokeweed mitogen (pwm) and a novel mit ... | 1985 | 3929565 |
cytotoxic factor production by kupffer cells elicited with lactobacillus casei and corynebacterium parvum. | the ability of kupffer cells, spleen macrophages, pulmonary macrophages, and peritoneal macrophages (pm) to produce cytotoxic factor (ctf) was investigated in vitro. the production of ctf by kupffer cells elicited with corynebacterium parvum (cp) or lactobacillus casei yit9018 (lc9018) was higher than that of spleen, pulmonary macrophages, or pm. in addition, oxygen radical (or) production by kupffer cells or pm was measured. the production of or by kupffer cells or pm was significantly augmente ... | 1985 | 3930066 |
the effect of combination treatment with alpha-difluoromethylornithine and corynebacterium parvum on b16 melanoma growth and tumoricidal effector cell generation in vivo. | the objective of the present investigation was to establish whether a known lymphoreticular-stimulating agent corynebacterium parvum would augment the established antitumor activity of alpha-difluoromethylornithine in vivo. furthermore, since c. parvum is known to boost cell mediated cytotoxicity, the effect of dfmo (dl-alpha-difluoromethylornithine x hcl x h2o) treatment was evaluated on macrophage and natural killer (nk) cell tumoricidal activity. dfmo administered alone, 1% or 2% in drinking ... | 1985 | 3933819 |
[experimental studies on the amplification of anti-tumor effects using bacterial dna-treated cancer cells, immunoactivators and interferon]. | | 1985 | 3934297 |
evaluation of the sterility and stability of insulin from multidose vials used for prolonged periods. | diabetic patients frequently use their insulin vials for prolonged periods, even though antibacterial preservatives used in multidose insulin vials are not required to be effective beyond 28 days. for this reason, we evaluated the antibacterial activity present in multidose insulin vials for up to 50 days. multidose lente insulin vials were inoculated with s. aureus and p. aeruginosa. vials incubated at room temperature (21 degrees c) were sterile by 48 hours, whereas when they were incubated at ... | 1985 | 3935593 |
arthropathic properties of cell wall polymers from normal flora bacteria. | peptidoglycan-polysaccharide (pg-ps) fragments were purified from cell walls of group d streptococci (streptococcus faecium, strains atcc 9790 and f-24) with a protocol which minimizes autolytic activity and tested for ability to induce arthritis in rats. pg-ps fragments from cell walls of other normal flora bacteria (peptostreptococcus productus, and propionibacterium acnes), group a streptococci, and pseudomurein-ps fragments from cell walls of methanobacterium formicicum, were similarly purif ... | 1986 | 3940996 |
ability of oral bacteria to degrade fibronectin. | the fibronectin-degrading ability of 116, mainly oral, strains was assayed by using plasma-derived fibronectin adsorbed to a polystyrene surface. ability to degrade fibronectin was revealed in strains of bacteroides gingivalis, bacteroides intermedius, bacteroides loeschii, staphylococcus aureus, staphylococcus epidermidis, peptococcus prevotii, clostridium sporogenes, and propionibacterium acnes. the fibronectinolytic activity of subgingival bacteriological samples was found to be related to th ... | 1986 | 3943910 |
the microbial flora of the continent cecal urinary reservoir, its stoma and the peristomal skin. | the bacteria occurring in reservoir urine, on the cutaneous stoma and on the abdominal skin were studied in 9 patients who had undergone urinary diversion via a continent cecal reservoir. aerobic and/or facultatively anaerobic bacteria were isolated persistently from reservoir urine in 3 patients but only occasionally in the remainder. these organisms were isolated in pure culture. anaerobic bacteria also were found, although infrequently and only in conjunction with facultative anaerobes. most ... | 1986 | 3944854 |
an action spectrum for blue and near ultraviolet inactivation of propionibacterium acnes; with emphasis on a possible porphyrin photosensitization. | | 1986 | 3952162 |
bacterial lipases and chronic blepharitis. | eyelids and conjunctivae of 36 normal individuals and 60 patients from six clinical groups of chronic blepharitis were cultured for aerobic and anaerobic bacteria. the most common species isolated were coagulase-negative staphylococci (c-ns) and propionibacterium acnes. all strains of these species, and all staphylococcus aureus strains isolated were tested for the ability to break down triglycerides, cholesterol esters, and fatty waxes. each strain was incubated independently with appropriate s ... | 1986 | 3957566 |
origin and differentiation of natural killer cells. ii. functional and morphologic studies of purified nk-1.1+ cells. | cells bearing the nk-specific marker nk-1.1 were purified from mouse spleens by utilizing a monoclonal anti-nk-1.1 antibody and cell sorting. in normal adult mice, all of the splenic nk activity against yac-1 cells was found in the nk-1.1+ fraction, whereas nk-1.1- cells were depleted of nk activity. the nk activity of sorted nk-1.1+ cells was enriched 15- to 30-fold over unfractionated spleen cells. light and electron microscopic studies of purified nk-1.1+ cells showed a homogeneous population ... | 1986 | 3958492 |
suppressive effects of 3-methylcholanthrene on the in vitro antitumor activity of naturally cytotoxic cells. | transient suppression of splenic natural killer (nk), natural cytotoxic (nc), and peritoneal macrophage cytotoxicity was observed following a single injection of 3-methylcholanthrene (3-mc) into c3h/hen mice. natural killer cell activity was depressed by 30-60% 4-6 d after injection of 1.0 mg 3-mc. levels of nk reactivity returned to normal 8 d post 3-mc injection, and no suppression of natural killing was seen when tested 6 wk after 3-mc treatment. 3-mc did not affect propionibacterium acnes au ... | 1986 | 3959116 |
adjuvant and antitumour activities of synthetic lipid a analogues. | the biological activities of synthetic glycolipids, which were chemically synthesized and based on the structure of lipid a of the lipopolysaccharide (lps) from escherichia coli, were examined with special reference to their adjuvant activity on the induction of delayed-type hypersensitivity, activity on the induction of tumour necrotic factor (tnf) and tumour regressive activity on line 10 hepatoma in strain 2 guinea pigs. among them, a compound structurally corresponding to free e. coli lipid ... | 1986 | 3962449 |
determination of protein in polysaccharide-antibody complexes. | we describe a simple and sensitive method for determining antibody protein complexed with polysaccharide using binding of amido-black to antigen-antibody complexes and bovine serum albumin as protein standard. the method, which is a modification of the method used for the determination of total nitrogen or the protein concentration in milk, has shown a consistent quantitative relationship with the lowry procedure and has been developed for its particular application to serological precipitates. | 1986 | 3963752 |
imbalanced accumulation of ribosomal rna in macrophages activated in vivo or in vitro to a cytolytic stage. | previous studies have shown that peritoneal murine macrophages activated in vivo and in vitro to a tumoricidal stage have a depressed rate of rna synthesis. in attempting to clarify the differences in rna metabolism between noncytotoxic and tumoricidal macrophages, we have studied the relative accumulation of various species of rna in macrophages activated in vivo and in vitro with the use of agarose gel electrophoresis. macrophages activated in vitro to a cytotoxic stage with supernatants conta ... | 1985 | 3965571 |
modulation of hepatocyte protein synthesis during co-cultivation with macrophage-rich peritoneal cells in vitro. | the etiology of hepatic failure associated with the multiple-system organ failure syndrome is poorly understood. because of indirect evidence suggesting that macrophages or kupffer's cells may play a role in this phenomenon, macrophage-rich peritoneal cells were co-cultured with isolated rat hepatocytes. following co-culture, the rate of hepatocyte protein synthesis, quantitated by counts per minute of tritiated leucine incorporated into protein, was significantly diminished. this modulation of ... | 1985 | 3977588 |
enhanced thermal response of a rat sarcoma by corynebacterium parvum. | corynebacterium parvum was investigated in the response of rat mc7 sarcoma to local waterbath hyperthermia. heat treatment of 1-1.5 cm3 foot tumors at 43 degrees c for 2 h resulted in complete regression of 71% of the tumors. the mc7 cure was reduced to 31% when the tumors were heated at 43 degrees c for 1.5 h. c. parvum (700 micrograms, i.v.) when given 1-3 days before tumor heating at 43 degrees c for 1.5 h increased the host phagocytic activity, and the tumor regression from 31% to 65% (p les ... | 1985 | 3978610 |
treatment of malignant pleural effusions with intrapleural corynebacterium parvum or tetracycline. | thirty two patients with malignant pleural effusion were randomly allocated to treatment with intrapleural corynebacterium parvum or tetracycline hydrochloride in an attempt to prevent symptomatic recurrence of pleural fluid. success in preventing recurrence of fluid at one month, using up to 2 doses of each drug, was 14 of 16 cases for corynebacterium parvum, 5 of 9 for tetracycline given via an intercostal needle, and 6 of 7 for tetracycline given through an intercostal tube. these difference ... | 1985 | 3979476 |
prevention of tumor metastasis after surgical removal of primary tumor by using in vitro activated macrophages. | in vitro activation of peritoneal macrophages by propionibacterium acnes and its immunotherapeutic potential for inhibiting metastasis was investigated. c3h/hen mouse adherent peritoneal exudate cells as a source of macrophages were treated in vitro with p. acnes. these p. acnes-activated macrophages were tested for tumoricidal activity in vitro and antimetastatic activity in vivo. in vitro cytotoxicity tests revealed that they had potent cytotoxicity. surgical resection of x5563 tumors in synge ... | 1985 | 3981817 |
comparative in vitro activity of cefbuperazone against anaerobic bacteria isolated from community hospitals. | the activity of cefbuperazone against 266 strains of anaerobic bacteria was determined by the agar dilution method and compared with cefoxitin, moxalactam, piperacillin, and clindamycin. all strains were recent clinical isolates from community hospitals. all agents tested showed good activity against bacteroides fragilis, fusobacterium spp., propionibacterium spp., clostridium septicum, clostridium perfringens, and the anaerobic, gram-positive cocci and gram-negative cocci. cefbuperazone, cefoxi ... | 1985 | 3985600 |
[conditions for porphyrin formation by propionic acid bacteria]. | porphyrin production by seven species of propionic acid bacteria (propionibacterium shermanii, its mutant p. shermanii m-82, p. technicum, p. vannielii, p. rubrum, p. thoenii and p. jensenii) was being studied. all the bacteria were cultivated on a glucose-peptone medium. a positive correlation between the amount of the produced porphyrins and the vitamin b12-synthetizing activity was observed for the most of species. exogenous delta-aminolevulinic acid stimulated the porphyrin accumulation, but ... | 1985 | 3991478 |
photodestruction of propionibacterium acnes porphyrins. | the fluorescence spectra of colonies of propionibacterium acnes were studied under various experimental conditions. the spectra contained peaks at 580 nm and 620 nm. these bands were due to two different components; the 580 nm component was likely to be a metalloporphyrin, and there are indications that the 620 nm component could be a coproporphyrin. the 580 nm fluorescence was destroyed by the combined action of light and oxygen (no destruction under strict anaerobic conditions). a dark period ... | 1985 | 3993179 |
carbohydrate-specific adhesion of bacteria to thin-layer chromatograms: a rationalized approach to the study of host cell glycolipid receptors. | conditions have been adapted for the binding of intact bacteria to glycosphingolipids in a thin-layer chromatogram. bacteria labeled externally with 125i or metabolically with other isotopes are layered on the plate and after repeated washing the bound bacteria are detected by autoradiography. using this technique several kinds of bacteria have been shown to adhere to the plate in a carbohydrate-specific way with practically no background binding. among the advantages of the method is the possib ... | 1985 | 3993927 |
corynebacterium parvum augments antibody production in splenectomized mice and mice with sham operations. | the antibody response to a variety of antigens, including pneumococcal polysaccharides, is diminished in splenectomized (splx) mice. we investigated the capacity for the biological response modifier corynebacterium parvum to augment antibody production in splx and sham-splx mice inoculated with pneumococcal polysaccharides and tetanus toxoid. as expected, antibody response to tetanus toxoid was similar in both splx mice and sham-splx mice. c. parvum augmented anti-tetanus toxoid antibody in both ... | 1985 | 3997248 |
production of tumour necrosis factor in listeria monocytogenes-infected animals. | both mice and rabbits injected intravenously with viable listeria monocytogenes and challenged with bacterial lipopolysaccharide release tumour necrosis factor (tnf) into the blood. optimal conditions for production of murine tnf using listeria were established. the cell-kill efficacy of listeria-tnf and of corynebacterium parvum-tnf are comparable. also, the two sera have similar spectra of activity; a wide variety of murine and human tumour cell lines are vulnerable while others are not affect ... | 1985 | 3997317 |
the quantity and function of pulmonary alveolar macrophages after splenectomy and corynebacterium parvum. | we have previously shown that corynebacterium parvum (c. parvum), a nonspecific immunomodulator, partially protects splenectomized and nonsplenectomized mice when challenged with aerosolized pneumococci. we report here the effects of both splenectomy and c. parvum on the phagocytic function of the lavageable pulmonary alveolar macrophage (pam). groups of young adult male sprague dawley rats underwent splenectomy or sham operation 3 weeks before injection of c. parvum 1.5 mg ip (or saline) per an ... | 1985 | 3999160 |
role of lipid a of endotoxin in the production of tumour necrosis factor. | bacterial lipopolysaccharide (lps) induces the release of tumour necrosis factor (tnf) into serum of mice previously infected with listeria monocytogenes or immunized with formalin-killed corynebacterium parvum. this release is greatly reduced by neutralisation of lipid a of lps with the antibiotic polymyxin b sulfate. the effect is dose-dependent. base-hydrolysed lps, which is devoid of lipid a, cannot induce tnf release. crude lipid a still retains its ability to induce tnf release but is sign ... | 1985 | 4000132 |
can technetium-labelled millimicrospheres be used to measure kupffer-cell function? an experimental study. | it has been suggested that sodium pertechnetate 99mtc millimicrospheres can be used to measure kupffer-cell function. we studied animals and humans to show whether the clearance and catabolism of 99mtc-labelled millimicrospheres can be used as a measure of kupffer-cell function. comparison with albumin 125i-microaggregates clearance of human serum albumin failed to demonstrate that they can be used for this purpose. we suggest that their blood clearance is mainly an expression of liver blood flo ... | 1985 | 4006979 |
[solubilizing effect of anionic detergents on cobalamin-protein compounds in propionibacterium shermanii cells]. | the effect of some anionic detergents on propionibacterium shermanii cells was studied. the correlation was investigated between detergent concentrations and the content of solubilized protein in cell-free extracts. sodium dodecyl sulfate, sodium deoxycholate and sodium cholate were shown to be capable of extracting cobalamin-protein and flavin-protein compounds from p. shermanii cells. | 1985 | 4010549 |
ganglioside alterations in stimulated murine macrophages. | a two-dimensional thin-layer chromatographic technique has been used to separate and display gangliosides from murine peritoneal macrophages in different functional states. resident macrophages have a relatively simple ganglioside pattern with about 15 resorcinol-positive spots. gangliosides from resident cells contained mostly (90%) n-glycolylneuraminic acid. thioglycolate-elicited and corynebacterium parvum-activated macrophages have much more complex patterns with about 40 resorcinol-positive ... | 1985 | 4016115 |
[studies on hepatocellular injury in experimentally induced acute hepatic failure--i. possible involvement of the activated adherent cells]. | | 1985 | 4021165 |
bacteria in the female breast. | subclinical infection may play a role in capsular formation around silicone breast implants. to assess the possibility of antibiotic prophylaxis for prevention of capsular formation, knowledge of bacteria present in the female breast tissue and the resistance pattern of these bacteria is needed. samples were taken from 25 patients (49 breasts) peroperatively during reduction mammaplasty with an impression pad method. the samples were placed in agar plates and incubated both aerobically and anaer ... | 1985 | 4023644 |
synergistic effects of combination sequential immunotherapies in a murine ovarian cancer model. | the antitumor effects of corynebacterium parvum in a murine ovarian teratocarcinoma model depend upon a sequential activation of neutrophils and macrophages within the peritoneal cavity. we studied the sequential administration of biological response modifiers that independently activate each phase of the response. tumor-challenged mice treated by i.p. injection of a pyridine-extracted fraction of cell-free propionibacterium acnes (pa-pe, 1400 micrograms) demonstrated prolonged survival in less ... | 1985 | 4028011 |
rejection of murine ovarian cancer following treatment with regional immunotherapy: correlations with a neutrophil-mediated activation of cytostatic macrophages. | rejection of the murine ovarian teratocarcinoma (mot) in c3heb/fej mice, following intraperitoneal (ip) treatment with corynebacterium parvum (c. parvum), is abrogated by injections of silica. we, therefore, investigated whether c. parvum-elicited macrophages affect mot targets in vitro. tumor-cytostatic, but not cytolytic, macrophages were detected in normal and tumor-challenged mice treated with c. parvum. the dose responsiveness and kinetics of macrophage activation strongly correlated with t ... | 1985 | 4028167 |
purification and partial amino acid sequence of rabbit tumor necrosis factor. | good production of tumor necrosis factor (tnf) in the rabbit was obtained using propionibacterium acnes iid 912 as a priming agent and subsequent administration of lipopolysaccharide. the physicochemical characteristics of rabbit tnf were very similar to those of murine tnf. the molecular weight of rabbit tnf was 39,000 as estimated by gel filtration, and 18,000 by sds-page. the isoelectric point was determined as ph 4.0 by isoelectric focusing. rabbit tnf was stable within the ph range of 5.5 t ... | 1985 | 4030139 |
repression of lymphatic metastasis by a second implant of the same tumor. | previous work in the rat d-12 fibrosarcoma model has shown that the incidence of macroscopic metastasis, located primarily in regional lymph nodes, is markedly increased after surgical removal of a primary subcutaneous tumor implant, in all probability by facilitating the outgrowth of already established micrometastases. the present work shows that a second implant of live d-12 tumor cells into a remote site effectively represses the outgrowth of macroscopic metastases. for the manifestation of ... | 1985 | 4030246 |
structure of acidic polysaccharide from cell wall of propionibacterium acnes strain c7. | the structure of polysaccharide prepared by lysozyme digestion from the cell wall of propionibacterium acnes strain c7 was examined. the polysaccharide fraction was composed of glucose, galactose, mannose, galactosamine, and diaminomannuronic acid in a molar ratio of 1:1:0.3:1:2. by smith degradation of the polysaccharide, diaminouronic acid-containing fractions were obtained, and the configuration of diaminouronic acid was identified as 2,3-diacetamido-2,3-dideoxymannuronic acid [man(nac)2a] by ... | 1985 | 4030744 |
experimental photoallergy to systemic drugs. | we have induced photoallergy in mice to systemically administered drugs, specifically sulfanilamide and chlorpromazine. mice were photosensitized to systemic sulfanilamide or chlorpromazine by i.p. administration of drug followed by uvb and uva irradiation of shaved flank skin, on two consecutive days. control mice received i.p. drug with no irradiation. in some experiments cyclophosphamide pretreatment, or intradermal corynebacterium parvum (propionibacterium acnes), was administered as an immu ... | 1985 | 4031536 |
[proliferative response of murine lymphocytes caused by the activity of amphiphilic molecules from propionibacterium acnes]. | splenic lymphocytes from mice treated with propionibacterium acnes cells as well as with their cell walls were found to be variably active on the lymphoproliferative responsiveness. furthermore, the effect of these bacterial agents on the ex vivo con a response of the lymphocytes showed a certain stimulation that was higher with oral treatments. in the same conditions the influence of these agents on the lps lymphocytes stimulation was almost without any statistical significance. in vitro blasto ... | 1985 | 4035056 |
further experience of corynebacterium parvum in malignant pleural effusion. | the outcome of drug induced pleurodesis has been evaluated in a non-randomised retrospective study of 67 patients with recurrent malignant pleural effusions treated during 1976-83. fourteen died within 30 days of treatment. of the remaining 53 patients, 26 studied during 1976-80 were treated with intrapleural mustine hydrochloride, while 27 studied during 1978-83 were treated with intrapleural corynebacterium parvum. there was no significant difference between the groups in age, sex, or tumour t ... | 1985 | 4035618 |
disappearance and reappearance of resident macrophages: importance in c. parvum-induced tumoricidal activity. | we have investigated the role of resident macrophages in the early tumoricidal response to c. parvum. the bacteria were labeled with fitc and resident cells were labeled in situ with blue fluorescent covaspheres to enable subsequent monitoring of cellular changes by flow cytometry. macrophages disappeared within 5 hr of administration of bacteria. at 24 hr, fibrinous adhesions containing double labeled macrophages were observed at numerous sites on the peritoneum. macrophages associated with lar ... | 1985 | 4038473 |
[alveolar lymphocyte proliferation in sarcoidosis patients induced by propionibacterium acnes]. | | 1985 | 4046241 |
[treatment of neoplastic pleural effusion by intrapleural administration of corynebacterium parvum vaccine]. | | 1985 | 4047949 |