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the expanded spectrum of bartonellosis in children.bartonella spp cause various clinical syndromes immunocompetent and immunocompromised hosts. domestic cats are the natural reservoir, and vectors of b henselae. b henselae infection usually occurs early in childhood, is generally asymptomatic, and in most cases revolves spontaneously. it may, however, produce a wide spectrum of clinical symptoms, the most frequent feature being cat-scratch disease. disseminated atypical b. henselae infection may follow cat-scratch disease alter a symptom-free pe ...200516102656
diagnosis of cat scratch disease with bartonella henselae infection in formalin-fixed paraffin-embedded tissues by two different pcr assays.cat scratch disease (csd) is commonly caused by bartonella henselae infection. clinical history and histologic findings are often insufficient to establish a definitive diagnosis of csd. we retrospectively studied formalin-fixed, paraffin-embedded (ffpe) lymph nodes from 35 patients with histologically suspected csd by 2 different pcr assays and immunohistochemistry (ihc). the first primer pair amplified a 163-bp fragment of the 16s rrna gene in 19 of the 35 cases (54%). the second primer pair a ...200516106195
characterization of the genome composition of bartonella koehlerae by microarray comparative genomic hybridization profiling.bartonella henselae is present in a wide range of wild and domestic feline hosts and causes cat-scratch disease and bacillary angiomatosis in humans. we have estimated here the gene content of bartonella koehlerae, a novel species isolated from cats that was recently identified as an agent of human endocarditis. the investigation was accomplished by comparative genomic hybridization (cgh) to a microarray constructed from the sequenced 1.93-mb genome of b. henselae. control hybridizations of labe ...200516109957
[diagnostic tests: cat-scratch disease]. 200516111236
interaction of bartonella henselae with endothelial cells promotes monocyte/macrophage chemoattractant protein 1 gene expression and protein production and triggers monocyte migration.bacillary angiomatosis (ba), one of the many clinical manifestations resulting from infection with the facultative intracellular bacterium bartonella henselae, is characterized by angiogenic lesions. macrophages have been identified as important effector cells contributing to the angiogenic process during b. henselae infection by infiltrating ba lesions and secreting vascular endothelial growth factor. monocyte-macrophage chemoattractant protein 1 (mcp-1) recruits macrophages to sites of inflamm ...200516113290
the transcriptional response of human endothelial cells to infection with bartonella henselae is dominated by genes controlling innate immune responses, cell cycle, and vascular remodelling.the bacterial pathogen bartonella henselae (bh) is responsible for a broad range of clinical manifestations, including the formation of vascular tumours as the result of pathogen-triggered vasoproliferation. in vitro, the interaction of bh with human umbilical vein endothelial cells (huvec) involves (i) cytoskeletal rearrangements in conjunction with bacterial internalization, (ii) nuclear factor kappab (nfkappab)-dependent proinflammatory activation, (iii) the inhibition of apoptosis, and (iv) ...200516113825
characterization and expression analysis of the groesl operon of bartonella bacilliformis.the groesl operon of bartonella bacilliformis, a facultative intracellular, gram-negative bacterium and etiologic agent of oroya fever, was characterized. sequence analysis revealed an operon containing two genes of 294 (groes) and 1632 nucleotides (groel) separated by a 55-nt intergenic spacer. the operon is preceded by a 72-nt orf (orf1) that encodes a hypothetical protein with homology to a portion of the hrca repressor for groesl. a divergent fumarate hydratase c (fumc) gene lies further ups ...200516126349
killed in action: microbiologists and clinicians as victims of their occupation. part 2: yellow fever and bartonellosis. 200516128394
cat-scratch disease in elderly patients.cat-scratch disease (csd) is mostly contracted by children and young adults. to our knowledge, csd in elderly patients has never been characterized, and it may be underrecognized in this age group.200516142661
potential limitations of the 16s-23s rrna intergenic region for molecular detection of bartonella species. 200516145180
[zoonotic diseases caused by bacteria of the genus bartonella genus: new reservoirs ? new vectors?].domestic animals and wildlife represent a large reservoir for bartonellae, at least eight species or subspecies of which have been reported to cause zoonotic infections. in addition, numerous orphan clinical syndromes are now being attributed to bartonella henselae infection. many mammalian species, including cats, dogs, rodents and ruminants are the main bartonellae reservoirs. cats are the main reservoir for b. henselae. it appears that domestic dogs, at least in non tropical regions, are more ...200516149211
molecular characterization of haemoparasites infecting bats (microchiroptera) in cornwall, uk.the presence of haemoparasites from the order piroplasmida and the genera bartonella and trypanosoma was assessed in the blood of 60 bats, belonging to 7 species, inhabiting sites across cornwall in southwest england. dna extracted from macerated heart tissue was incorporated into taxon-specific polymerase chain reactions (pcrs) and amplification products were sequenced as a means of identifying, or assigning an identity, to detected haemoparasites. a piroplasmida species was detected in 6 pipis ...200516174413
role of dendritic cell-derived cxcl13 in the pathogenesis of bartonella henselae b-rich granuloma.dendritic cells (dcs) initiate adaptive immunity and regulate the inflammatory response by producing inflammatory chemokines. this study was aimed to elucidate their role in the pathogenesis of the suppurative granuloma induced by bartonella henselae infection, which characterizes cat scratch disease (csd). in vitro dc infection by b. henselae results in internalization of bacteria, phenotypic maturation with increased expression of hla-dr and cd86, and induction of cd83, cd208, and ccr7. in com ...200616189275
bartonella spp. seroprevalence in healthy swedish blood donors.serum samples were collected from healthy blood donors in 5 regions in sweden in 1999, i.e. from the local blood centres (collecting facilities) in boden, jönköping, lund, skövde, and uppsala. in total, 498 serum samples (63% males, 37% females) were received and tested by immunofluorescence assay for antibodies against b. elizabethae, b. grahamii, b. henselae (houston-1), b. henselae (marseille), b. quintana, and b. vinsonii subsp. vinsonii. an overall bartonella spp. seroprevalence of 16.1% wa ...200516191889
case records of the massachusetts general hospital. case 30-2005. a 56-year-old man with fever and axillary lymphadenopathy. 200516192484
use of shell-vial cell culture assay for isolation of bacteria from clinical specimens: 13 years of experience.the shell-vial culture assay is performed routinely in our laboratory. recently we revisited our experience of using the shell-vial culture assay for the isolation of microorganisms from various clinical samples. over a 13-year period, we have isolated 580 bacterial strains (5%) from 11,083 clinical samples tested. over the same period, 285 isolates of rickettsiae, bartonellae, or coxiella burnetii were cultured from a total of 7,102 samples tested. these isolates include 55 rickettsia sp. isola ...200516207953
contribution of systematic serological testing in diagnosis of infective endocarditis.despite progress with diagnostic criteria, the type and timing of laboratory tests used to diagnose infective endocarditis (ie) have not been standardized. this is especially true with serological testing. patients with suspected ie were evaluated by a standard diagnostic protocol. this protocol mandated an evaluation of the patients according to the modified duke criteria and used a battery of laboratory investigations, including three sets of blood cultures and systematic serological testing f ...200516207989
antibiotic therapy for nontuberculous mycobacterial cervicofacial lymphadenitis.to evaluate the efficacy of antibiotic treatment of nontuberculous mycobacterial (ntm) cervicofacial lymphadenitis, both as an alternative and as adjuvant to surgical excision.200516222188
detection of bartonella henselae dna by polymerase chain reaction in a patient with cat scratch disease: a case report.we report a case of cat scratch disease caused by bartonella henselae in korea. a 25-yr-old woman developed left cervical lymphadenopathy with history of contact with a dog. the cervical lymphadenopathy persisted for 1 month and resolved gradually and spontaneously. serologic test was not done during the acute stage of the disease. immunofluorescent antibody test performed during the convalescent stage was positive for b. henselae. to confirm b. henselae infection, polymerase chain reaction (pcr ...200516224169
isolation of bacteriophages from bartonella vinsonii subsp. berkhoffii and the characterization of pap31 gene sequences from bacterial and phage dna.bacteriophages enhance bacterial survival, facilitate bacterial adaptation to new environmental conditions, assist in the adaptation to a new host species, and enhance bacterial evasion or inactivation of host defense mechanisms. we describe the detection and purification of a novel tailed bacteriophage from bartonella vinsonii subsp. berkhoffii, which was previously described as a bacteriophage-negative species. we also compare b. vinsonii subsp. berkhoffi pap31 bacteriophage gene sequences to ...200516254445
cat-scratch disease-associated arthropathy.to characterize the articular manifestations of cat-scratch disease (csd) and to evaluate the long-term clinical outcome of those manifestations.200516255053
images in emergency medicine. parinaud's oculoglandular syndrome. 200516271667
functional interactions between type iv secretion systems involved in dna transfer and virulence.this paper reports an analysis of the functional interactions between type iv secretion systems (t4ss) that are part of the conjugative machinery for horizontal dna transfer (ct4ss), and t4ss involved in bacterial pathogenicity (pt4ss). the authors' previous work showed that a conjugative coupling protein (t4cp) interacts with the virb10-type component of the t4ss in order to recruit the protein-dna complex to the transporter for conjugative dna transfer. this study now shows by two-hybrid analy ...200516272374
bartonella quintana endocarditis in east africa.bartonella quintana endocarditis is characterized by sub-acute evolution and severe valvular damage, and is associated with homelessness, alcoholism, and lice infestation. we present a case of b. quintana endocarditis in an ethiopian immigrant without known risk factors for disease acquisition. this is the first case of b. quintana endocarditis reported from east africa.200516275550
evaluation of a western blot method for the detection of yersinia antibodies: evidence of serological cross-reactivity between yersinia outer membrane proteins and borrelia burgdorferi.yersinia enterocolitica and yersinia pseudotuberculosis have been identified as causative organisms of reactive arthritis in humans. we evaluated a western blot assay which uses yersinia outer membrane proteins as antigens for the detection of yersinia antibodies as a replacement for the complement fixation (cf) assay. clinical agreement, sensitivity, and specificity were determined by testing 19 positive and 21 negative serum samples by the cf assay, western blot assay, and enzyme-linked immuno ...200516275939
prevalence of bartonella species, haemoplasma species, ehrlichia species, anaplasma phagocytophilum, and neorickettsia risticii dna in the blood of cats and their fleas in the united states.ctenocephalides felis were killed and collected from 92 cats in alabama, maryland, and texas. the fleas and blood from the corresponding cat were digested and assessed in polymerase chain reaction assays that amplify dna of ehrlichia species, anaplasma phagocytophilum, neorickettsia risticii, mycoplasma haemofelis, 'candidatus m haemominutum' and bartonella species. dna consistent with b henselae, b clarridgeiae, m haemofelis, or 'candidatus m haemominutum' was commonly amplified from cats (60.9 ...200616290092
detection of bartonella species from ticks, mites and small mammals in korea.we investigated the prevalence of bartonella infections in ticks, mites and small mammals (rodents, insectivores and weasels) collected during 2001 through 2004, from various military installations and training sites in korea, using pcr and sequence analysis of 16s rrna, 23s rrna and groel heat shock protein genes. the prevalence of bartonella spp. was 5.2% (n = 1,305 sample pools) in ticks, 19.1% (n = 21) in mesostigmatid mites and 13.7% (n = 424 individuals) in small mammals. the prevalence wi ...200516293997
[determination of antibody titer to bartonella henselae by indirect fluorescence antibody assay using b. henselae from domestic cats as antigen]. 200516296330
codon and amino acid usage in two major human pathogens of genus bartonella--optimization between replicational-transcriptional selection, translational control and cost minimization.intra-genomic variation in synonymous codon and amino acid usage in two human pathogens bartonella henselae and b. quintana has been carried out through multivariate analysis. asymmetric mutational bias, coupled with replicational-transcriptional selection, has been identified as the prime selection force behind synonymous codon selection--a characteristic of the genus bartonella, not exhibited by any other alpha-proteobacterial genome. distinct codon usage patterns and low synonymous divergence ...200516303741
is pyogenic granuloma associated with bartonella infection?lobular capillary hemangioma and bacillary angiomatosis due to bartonella infection share several clinical and histopathologic characteristics. we sought to determine whether lobular capillary hemangioma is caused by the same agent as bacillary angiomatosis. forty-five pathology specimens with a histologic diagnosis of lobular capillary hemangioma obtained from patients with the same clinical diagnosis were tested by immunohistochemistry and polymerase chain reaction for the presence of dna elem ...200516310070
prevalence of human pathogens in cat and dog fleas in new zealand.to provide further information on the prevalence of rickettsia felis, bartonella hensela, and b. clarridgeiae in cat and dog fleas in new zealand and their distribution in the country.200516311612
cat scratch disease encephalopathy in an immunocompetent patient.cat scratch disease (csd) is typically a self-limited regional lymphadenopathy in children and young adults that is caused by bartonella henselae. the majority of csd cases resolve spontaneously; however, many systemic complications have been described. we report an unusual case of csd presenting as an epitrochlear arm mass and complicated by encephalopathy. identification of b. henselae dna in the affected lymph node and cerebrospinal fluid confirmed the diagnosis of csd. systemic antibiotic th ...200516314249
polymerase chain reaction (pcr) survey for rickettsias and bartonellas in ticks from new zealand. 200516317451
tickborne pathogen detection, western siberia, russia.ixodes persulcatus (n = 125) and dermacentor reticulatus (n = 84) ticks from western siberia, russia, were tested for infection with borrelia, anaplasma/ehrlichia, bartonella, and babesia spp. by using nested polymerase chain reaction assays with subsequent sequencing. i. persulcatus ticks were infected with borrelia burgdorferi sensu lato (37.6% +/- 4.3% [standard deviation]), anaplasma phagocytophilum (2.4% +/- 1.4%), ehrlichia muris (8.8% +/- 2.5%), and bartonella spp. (37.6% +/- 4.3%). d. re ...200516318722
bartonella quintana and rickettsia felis in gabon.we detected rickettsia felis dna in ctenocephalides felis and bartonella quintana dna in 3 pulex irritans fleas taken from a pet cercopithecus cephus monkey in gabon, sub-saharan africa. this is the first report of b. quintana in the human flea.200516318727
evidence for louse-transmitted diseases in soldiers of napoleon's grand army in vilnius.many soldiers in napoleon's grand army died of infectious diseases during its retreat from russia. because soldiers were commonly infested with body lice, it has been speculated that louse-borne infectious diseases, such as epidemic typhus (caused by rickettsia prowazekii), were common.200616323139
pattern visual evoked potentials in eyes with disc swelling due to cat scratch disease-associated neuroretinitis.to evaluate optic nerve function by pattern visual evoked potentials (veps) in eyes with optic disc swelling due to neuroretinitis associated with cat scratch disease (csd).200516328936
aortic valve endocarditis with bartonella quintana - a rare entity.endocarditis caused by bartonella species is difficult to diagnose and still remains a rare entity. therefore, a young male patient undergoing aortic valve replacement for culture-negative endocarditis is reported in whom the diagnosis of a bartonella quintana infection was made with a great delay postoperatively.200516331372
[detection of the bartonella dna by the method of nested pcr in patients after tick bites in novosibirsk region].arthropod-borne bacterial pathogen bartonella dna was detected in human blood cells after tick bites in summer 2003 and 2004 in novosibirsk region by nested pcr with primers specific to groel gene of hsp60 protein. comparative assay of 190 p.b. of long pcr fragment revealed that the nucleotide sequences might belong to bartonella henselae and bartonella quintana.200516334219
bacterial 16s ribosomal dna in house dust mite cultures.allergen extracts prepared from dermatophagoides farinae contain significantly more endotoxin than dermatophagoides pteronyssinus extracts, and extracts from both mite extracts contain more endotoxin than pollen extracts. attempts to culture bacteria from mite cultures have failed to establish the sources of the endotoxin.200516337462
disseminated cat scratch disease with vertebral osteomyelitis and epidural abscess.a 5-year-old boy with cat scratch disease presented with fever of unknown origin and osteomyelitis of the thoracic spine and epidural abscess. he did not have localizing signs or symptoms. computed tomography of the abdomen, which was initially negative, showed hepatosplenic disease. cat scratch disease has variable systemic presentations and should be included in the differential diagnosis of fever of unknown origin if an epidemiologic risk factor is present.200516351038
plasmacytoid monocytes in cat scratch disease with special reference to the histological diversity of suppurative lesions.it has been suggested that plasmacytoid monocytes (pmos) play an essential role in t-cell-dependent immune response. indeed, numerous pmos are found in close topographical association with epithelioid cell granulomas in hypersensitivity-type granulomas, such as tuberculosis and sarcoidosis. the key pathologic process in cat scratch disease (csd) usually involves a b-cell-associated granulomatous reaction. histologically, csd appears to exhibit a histopathologic diversity, including suppurative l ...200616352403
[cat-scratch disease as cause of lymphadenitis colli].cat-scratch disease as cause of lymphadenitis colli. cat-scratch disease is a frequent cause of lymphadenitis colli. it mainly affects children and adolescents younger than 21 years. since the clinical picture is not characteristic, a history of contact to cats or kittens is highly valuable for diagnosing the disease. major aspects of the disease concerning epidemiology, diagnostic procedures, clinical presentation and therapy are discussed.200516358204
[inguinal mass as presentation of cat-scratch disease]. 200516358433
comparative proteomic analysis of b. henselae houston and b. henselae marseille by two-dimensional gel electrophoresis.to compare the protein difference between b. henselae houston and b. henselae marseille by two-dimensional gel electrophoresis.200516370318
[cat-scratch disease].to study the etiology, epidemiology, clinical and pathological features, diagnose and treatment of cat-scratch disease(csd).200516375109
clinicopathologic findings in dogs seroreactive to bartonella henselae antigens.to assess the potential clinical relevance of seroreactivity to bartonella henselae antigens in dogs.200516379647
[prevalence of serum antibodies against bartonella ssp. in a health population from the south area of the seville province].to know the prevalence of serum antibodies against bartonella spp. in a healthy population from south of spain.200516381097
bartonella quintana endocarditis with positive serology for coxiella burnetii.both bartonella quintana and coxiella burnetii are known to cause of blood culture negative endocarditis. in such case, the diagnosis is usually established by serology. a case of bartonella quintana endocarditis is described where the serology was falsely positive for coxiella burnetii. this case demonstrates the difficulty in distinguishing these two etiologic agents by routine serologic testing.200616386308
antiangiogenic effect of erythromycin: an in vitro model of bartonella quintana infection.bartonella quintana, the etiological agent of bacillary angiomatosis (ba), causes endothelial cell proliferation. erythromycin has dramatic effects on ba, and the effects are largely unexplained by the compound's bacteriostatic properties. our aim here was to evaluate the possibility that erythromycin alters angiogenesis.200616388485
effects of cow age and pregnancy on bartonella infection in a herd of dairy cattle.bartonella spp. are small hemotropic bacteria infecting mammals. four bartonella species have been recently described in cattle and wild ruminants. to date, the biology and possible pathogenic role of bartonella species isolated from ruminants are poorly understood. therefore, a dairy herd of 448 cows and heifers was surveyed in order to establish the prevalence of bartonella bovis and b. chomelii infections, the level of bacteremia, and the relationship between bacteremia and age or pregnancy s ...200616390945
first isolation of bartonella alsatica from a valve of a patient with endocarditis.we report the first documented case of endocarditis in a man infected with bartonella alsatica, which causes bacteremia in healthy wild rabbits. b. alsatica was identified by serology and culture and by pcr of an aortic valve specimen. b. alsatica should be added to the list of zoonotic agents of blood culture-negative endocarditis.200616390990
bartonella henselae infection associated with guillain-barre syndrome.this is the first report of guillain-barré syndrome (gbs) related to bartonella henselae infection. a 10-year-old girl had difficulty walking and marked myalgia. the search for all causes known to trigger gbs was negative. she was treated with intravenous immunoglobulins and recovered. because she lived in a rural area and had a history of kitten contact, a specific serology for b. henselae infection was performed and confirmed an ongoing infection. she did not show any clinical typical feature ...200616395116
[multifocal chorioretinitis, papillitis, and recurrent optic neuritis in cat-scratch disease].to evaluate the causative factor for multifocal chorioretinitis, papillitis, and recurrent optic neuritis. a 41-year-old patient presenting multifocal choroiditis, papillitis, and recurrent optic neuritis was evaluated with funduscopy, angiography (fa), optical coherence tomography (oct), visual evoked potentials (vep), and numerous blood laboratory tests. fa and oct showed multifocal pigment epithelial detachments. vep showed typical changes for optic neuritis and papillitis. indirect fluoresce ...200516395190
[abrupt visual loss in children. three case studies of ocular bartonellosis].to evaluate the severity and progression of neuroretinitis in children caused by bartonella.200516395224
purification of the yada membrane anchor for secondary structure analysis and crystallization.non-fimbrial adhesins, such as yersinia yada, moraxella uspa1 and a2, haemophilus hia and hsf, or bartonella bada, represent an important class of molecules by which pathogenic proteobacteria adhere to their hosts. they form trimeric surface structures with a head-rod-anchor architecture. whereas their head and rod domains may be of heterologous origin, their anchor domains are homologous and display the properties of autotransporters. conflicting topology models exist for these membrane anchors ...200616405993
dna examination of ancient dental pulp incriminates typhoid fever as a probable cause of the plague of athens.until now, in the absence of direct microbiological evidence, the cause of the plague of athens has remained a matter of debate among scientists who have relied exclusively on thucydides' narrations to introduce several possible diagnoses. a mass burial pit, unearthed in the kerameikos ancient cemetery of athens and dated back to the time of the plague outbreak (around 430 bc), has provided the required skeletal material for the investigation of ancient microbial dna.200616412683
rodent-associated bartonella in saskatchewan, canada.six species of wild rodents were sampled at 10 sites in 2002 and 2003 to determine the prevalence of bartonella infections in rodent communities near saskatoon, saskatchewan, canada. isolates were characterized genotypically and compared with isolates found at other locations. of 104 wild rodents examined, 57% were infected with bartonella and prevalence within species varied from 49% for richardson's ground squirrels (spermophilus richardsonii) to 90% for franklin's ground squirrels (s. frankli ...200516417436
kikuchi's disease or kikuchi's syndrome? 200616421809
transient monoclonal gammopathy in a patient with bartonella quintana endocarditis.monoclonal gammopathy has been reported rarely in association with infectious diseases. viral infection has been the most frequently reported. we report a case of bartonella quintana endocarditis in a 45-year-old homeless male associated with a monoclonal igg kappa gammopathy. the gammopathy disappeared after 8 months of antibiotics while the bartonella antibody titre was decreasing. this correlation suggests a causative role for b. quintana for the monoclonal gammopathy. to the best of our know ...200616432867
serological survey of vector-borne zoonotic pathogens in pet cats and cats from animal shelters and feral colonies.although cats and their arthropod parasites can sometimes be important sources of zoonotic diseases in humans, the extent of exposure among various cat populations to many potential zoonotic agents remains incompletely described. in this study, 170 domestic cats living in private homes, feral cat colonies, and animal shelters from california and wisconsin were evaluated by serology to determine the levels of exposure to a group of zoonotic vector-borne pathogens. serological positive test result ...200616434226
confirmation of diagnosis of cat scratch disease by immunohistochemistry. 200616434905
[diagnostic imaging (257). a boy with a swollen inguinal gland].in a 4-year-old boy with left-sided inguinal lymphadenopathy and a papule on his left lower leg situated at the end of a scar of a probable cat scratch, cat scratch disease was confirmed serologically.200616440563
prevalence of selected infectious disease agents in cats from arizona.the objective of this study was to use polymerase chain reaction (pcr) assays to determine the prevalence of ehrlichia species, anaplasma phagocytophilum, mycoplasma haemofelis, 'candidatus mycoplasma haemominutum' and bartonella species from feral and relinquished cats in phoenix and nogales, arizona. dna from one or more of the organisms was amplified from 31 of 112 blood samples (27.7%). dna consistent with bartonella clarridgeiae 15 (13.4%), bartonella henselae 14 (12.5%), 'candidatus m haem ...200616443383
antibodies to rickettsia rickettsii, rickettsia typhi, coxiella burnetii, bartonella henselae, bartonella quintana, and ehrlichia chaffeensis among healthy population in minas gerais, brazil.rickettsial diseases except those belonging to spotted fever group rickettsioses are poorly studied in south america particularly in brazil where few epidemiological reports have been published. we describe a serosurvey for rickettsia rickettsii, r. typhi, coxiella burnetii, bartonella henselae, b. quintana, and ehrlichia chaffeensis in 437 healthy people from a brazilian rural community. the serum samples were tested by indirected micro-immunoflourescence technique and a cutoff titer of 1:64 wa ...200616444416
[presence of bartonella henselae in cats: natural reservoir quantification and human exposition risk of this zoonoses in chile].the availability of a serologic test for cat scratch disease in humans has allowed the diagnosis of an increasing number of cases of this disease in chile.200616446874
hepatic peliosis (bacillary angiomatosis) in aids: ct findings. 200616447083
bartonella quintana associated neuroretinitis.we report an observational case of bartonella quintana-associated neuroretinitis. the patient had a positive igm ifa titer for bartonella quintana early in the disease. after treatment, the neuroretinitis and igm resolved. given the patient's history, symptoms, response to treatment, and igm course, we believe his neuroretinitis was secondary to bartonella quintana.200616449005
bacillary angiomatosis associated with pseudoepitheliomatous hyperplasia.bacillary angiomatosis is an opportunistic bacterial infection caused by either bartonella henselae or b. quintana. the classic histologic presentation of bacillary angiomatosis involves three components: a lobular proliferation of capillaries with enlarged endothelial cells, neutrophilic debris, and clumps of finely granular material identified as bacteria with staining techniques. pseudoepitheliomatous hyperplasia is a histologic reaction pattern characterized by epithelial proliferation in re ...200616456322
ocular manifestation of cat-scratch disease in hiv-positive patients.to characterize ocular manifestations of cat-scratch disease in hiv-positive patients.200616458711
a bartonella vinsonii berkhoffii typing scheme based upon 16s-23s its and pap31 sequences from dog, coyote, gray fox, and human isolates.since the isolation of bartonella vinsonii subspecies berkhoffii from a dog with endocarditis in 1993, this organism has emerged as an important pathogen in dogs and as an emerging pathogen in people. current evidence indicates that coyotes, dogs and gray foxes potentially serve as reservoir hosts. based upon sequence differences within the 16s-23s its region and pap31 gene, we propose a classification scheme that divides b. vinsonii subsp. berkhoffii isolates into four distinct types. two conse ...200616460911
antimicrobial susceptibility of bartonella henselae using etest methodology.bartonella henselae is a fastidious slow growing pathogen which is seldom cultured in the laboratory. previous descriptions of antimicrobial susceptibility have been largely limited to feline isolates and/or laboratory reference strains, with no accounting for genotypic or phenotypic diversity.200616464897
a serological study of exposure to arthropod-borne pathogens in dogs from northeastern spain.there is limited information regarding the prevalence of many vector borne pathogens in europe and especially in spanish dogs. we investigated 206 sick and 260 clinically healthy dogs from three different regions in northeastern spain for antibodies to rickettsia conorii (rc), ehrlichia canis (ec), anaplasma phagocytophilum (ap), bartonella henselae (bh), bartonella vinsonii subsp. berkhoffii (bvb), leishmania infantum (li) and borrelia burgdorferi (bb) and for antigen of dirofilaria immitis (di ...200616472522
antibodies to bartonella vinsonii subsp. berkhoffii in moroccan dogs.bartonella vinsonii subsp. berkhoffii is a fastidious microorganism that has been recognized as an emerging human and canine pathogen. we report for the first time on the prevalence of antibodies to b. vinsonii subsp. berkhoffii in domestic dogs from morocco. the overall seroprevalence was 38% (56 of 147 dogs tested). most of the seropositive dogs were stray dogs from rabat (36%, 8 of 22) and khenifra (47%, 47 of 101). antibodies against b. vinsonii subsp. berkhoffii were found infrequently amon ...200616474074
bartonellae as elegant hemotropic parasites.bartonella species are hemotropic bacterial parasites of a wide range of mammals that occasionally cause disease in humans. the low prevalence of clinical manifestations compared to the high prevalence of infection underlines the elegance of these parasites that carefully exploit their hosts in a manner that optimizes their transmission. recent research efforts have begun to determine the strategies involved in this exploitation, and significant progress has been made in unraveling an unusually ...200516481527
production of recombinant protein pap31 and its application for the diagnosis of bartonella bacilliformis infection.tropical bartonellosis is a highly fatal epidemic and endemic infectious disease that occurs throughout the communities of the andes mountains in south america. the disease is caused by the facultative intracellular bacteria, bartonella bacilliformis. the emergence of bartonellosis in new geographic areas and an increase in the number of reported cases suggest the need for a rapid test for epidemiologic study and investigation of the disease burden. the objective of this research is to develop a ...200516481528
bartonella bacilliformis groel: effect on growth of human vascular endothelial cells in infected cocultures.bartonella are the only bacteria known to induce angioproliferative lesions of the human vasculature and liver during infection. previous work from our lab suggests that groel participates in the mitogenic response observed in huvec cultures supplemented with the soluble fraction of bartonella bacilliformis. work in this study shows that exposure to high concentrations of the fraction is actually cytotoxic for huvecs. to analyze this phenomenon, live b. bacilliformis-huvec cocultures were employ ...200516481529
occurrence of bartonella henselae and bartonella quintana among human immunodeficiency virus-infected patients.the purpose of this study was to determine the seroprevalence against bartonella henselae and bartonella quintana among a risk group, patients with hiv infection, and to identify the epidemiological factors involved. our data indicate that the prevalence of bartonella infection among hiv-infected patients is much greater than that in the healthy population of the same area and that bartonella infection should be considered in the differential diagnosis for patients with hiv disease.200516481530
bacillary angiomatosis caused by bartonella quintana.bacillary angiomatosis is a disorder of neovascular proliferation involving skin and lymph nodes of immunosuppressed patients. bartonella henselae or bartonella quintana have been inculpated as causative by direct culture or pcr amplification of dna sequences. here, we report the clinical evolution of a patient infected with human immunodeficiency virus (hiv) whose b. quintana infection was diagnosed by pcr.200516481531
molecular screening of bartonella species in rodents from the russian far east.a potentially broad-range pcr system for glta gene amplification has been designed. a nearly full (992 bp) sequence of the glta gene was amplified in two steps. dna was extracted from organs (spleen and liver) of four mostly spread species of rodents: apodemus agrarius and apodemus peninsulae mice; clethrionomys rufocanus and microtus fortis voles. all amplicons have been sequenced. the incidence of bartonella species in rodents was 73%, 60%, 60%, and 83% for the above-mentioned rodents, respect ...200516481532
characterization of genes involved in long-term bacteremia in mice by bartonella birtlesii. 200516481533
bartonella henselae in porpoise blood.we report detection of bartonella henselae dna in blood samples from 2 harbor porpoises (phocoena phocoena). by using real-time polymerase chain reaction, we directly amplified bartonella species dna from blood of a harbor porpoise stranded along the northern north carolina coast and from a pre-enrichment blood culture from a second harbor porpoise. the second porpoise was captured out of habitat (in a low-salinity canal along the northern north carolina coast) and relocated back into the ocean. ...200516485476
bartonella quintana in cynomolgus monkey (macaca fascicularis).we identified a bartonella quintana strain by polymerase chain reaction amplification, cloning, and sequencing of dna extracted from lysed erythrocytes and cultured colonies grown from peripheral blood collected from a captive-bred cynomolgus monkey (macaca fascicularis). this report describes naturally acquired b. quintana infection in a nonhuman primate.200516485482
production of recombinant bartonella henselae 17-kda protein for antibody-capture enzyme-linked immunosorbent assay.the bartonella henselae 17-kda protein was expressed in a prokaryotic expression system as a histidine-tagged fusion protein and was purified. the target gene was cloned into a recombinant expression construct, ptri-17kd. the expressed protein was purified to near homogeneity by a nickel-agarose column chromatography. protein recovery was estimated to be 2.9 mg from 100 ml of bacterial culture. the purified 17-kda protein was recognized by serum from patients infected with b. henselae and barton ...200616490335
bartonella quintana characteristics and clinical management.bartonella quintana, a pathogen that is restricted to human hosts and louse vectors, was first characterized as the agent of trench fever. the disease was described in 1915 on the basis of natural and experimental infections in soldiers. it is now recognized as a reemerging pathogen among homeless populations in cities in the united states and europe and is responsible for a wide spectrum of conditions, including chronic bacteremia, endocarditis, and bacillary angiomatosis. diagnosis is based on ...200616494745
epidemiological studies on bartonella quintana infections among homeless people in tokyo, japan.in an epidemiological investigation of trench fever in japan, we compared the seroprevalence of bartonella quintana in homeless people and in the general population. in homeless rescue outreach programs held in tokyo from may 2001 to march 2003, 151 blood samples were taken from non-hospitalized homeless people. the prevalence of igm and igg antibodies against b. quintana in these people was compared with that in 200 healthy blood donors using a commercially available indirect fluorescent antibo ...200616495631
localized bacillary angiomatosis in the oral cavity: observations about a neoplasm with atypical behavior. description of a case and review of the literature.bacillary angiomatosis is a rather frequent infectious pathology appearing mainly in the skin but can also affect the liver, spleen, heart, bones, lungs, muscles, central nervous system and other organs. the localization of the lesion in the oral cavity is rather rare, as it is evident in the literature. bacillary angiomatosis can be clinically similar to the kaposi's sarcoma and histologically confused with angiosarcoma, epitheloid hemangioma and pyogenic granuloma. a case of bacillary angiomat ...200616495874
first molecular evidence of bartonella quintana in pediculus humanus capitis (phthiraptera: pediculidae), collected from nepalese children.trench fever is a body louse-borne disease caused by bartonella quintana brenner. the recent status of louse infestation in nepalese children is not well known. we collected head and body lice, pediculus humanus capitis de geer and pediculus humanus humanus l., respectively, from 30 children, including 11 cases of double infestation with both head and body lice. detection of b. quintana in both louse species identified was carried out by polymerase chain reaction (pcr). pcr products with b. quin ...200616506456
bartonella henselae bacilli detected in vitrectomy aspirates in a patient with massive vitreous opacity with total retinal detachment.to present endophthalmitis-like massive vitreous opacity as a manifestation of cat-scratch disease.200616507491
do dogs harbour risk factors for human breast cancer?we ask consulting patients regularly whether they keep pets in order to identify zoonotic factors. it became apparent that patients with breast carcinoma (n=69) owned significantly more often dogs but not cats compared to age matched female controls. we compared the frequencies of dog and pet ownership with data from public available statistics on women (n=1320) of the same age group in bavaria. the most striking result was that more than twice the number of patients kept dogs permanently in the ...200616516398
brucella outer membrane protein omp31 is a haemin-binding protein.the expression of haemin-binding proteins (hbps) in the outer membrane is one of the strategies used by gram-negative bacteria to obtain iron from the host. no hbp has been described in brucella spp. we investigated whether omp31, an outer membrane protein from brucella with homology to hbps from bartonella quintana, is an hbp. soluble recombinant omp31 bound specifically to haemin-agarose, while an unrelated brucella protein (sura) did not. a similar experiment showed that native omp31 found in ...200616517201
molecular detection of bartonella quintana, b. elizabethae, b. koehlerae, b. doshiae, b. taylorii, and rickettsia felis in rodent fleas collected in kabul, afghanistan.the prevalences of bartonella spp. and rickettsia spp. were investigated using molecular methods in 77 rodent fleas collected in november 2002 by the french forces detachment in kabul, afghanistan. overall, bartonella dna was detected in 15.5% of gerbil fleas and 40.5% of rat fleas, whereas rickettsia felis was found in 9% of gerbil fleas. we described for the first time in this country bartonella quintana, b. koehlerae, b. taylorii, and rickettsia felis in fleas from the gerbil species meriones ...200616525103
type iv secretion systems and their effectors in bacterial pathogenesis.type iv secretion systems (t4sss) are membrane-associated transporter complexes used by various bacteria to deliver substrate molecules to a wide range of target cells. t4sss are involved in horizontal dna transfer to other bacteria and eukaryotic cells, in dna uptake from or release into the extracellular milieu, in toxin secretion and in the injection of virulence factors into eukaryotic host target cells by several mammalian pathogens. rapid progress has been made towards defining the structu ...200616529981
etiology of tick-borne febrile illnesses in adult residents of north-eastern poland: report from a prospective clinical study.febrile tick-borne diseases can be caused by different pathogens. the study objective was to prospectively determine the etiology of infection among adults exposed to tick bite and to detect acute human granulocytic anaplasmosis (hga), a recently emerging infection, in north-eastern poland. we evaluated 68 patients (34 males and 34 females, mean age 44.2+/-15 years) who had fever within 4 weeks after a tick bite. paired sera were tested for antibodies against tick-borne encephalitis virus, borre ...200616530481
predominant t helper 2 immune responses against bartonella henselae in naturally infected cats.this study was conducted to explicate the mechanism of long-term bacteremia in bartonella henselae-infected cats by the examining host immune responses. blood samples were collected from three naturally infected cats and the igg antibody titers and the cytokine responses in peripheral blood mononuclear cells (pbmc) were examined by quantitative reverse transcriptase-polymerase chain reactions (rt-pcr). relapsing bacteremia was found in two of the three cats during the examination period. the qua ...200616547414
anti-francisella tularensis dna aptamers detect tularemia antigen from different subspecies by aptamer-linked immobilized sorbent assay.aptamers are powerful candidates for molecular detection of targets due to their unique recognition properties. these affinity probes can be used to recognize and bind to their targets in the various types of assays that are currently used to detect and capture molecules of interest. they are short single-stranded (ss) oligonucleotides composed of dna or rna sequences that are selected in vitro based on their affinity and specificity for the target. using combinatorial oligonucleotide libraries, ...200616550191
pet ownership in immunocompromised children--a review of the literature and survey of existing guidelines.pet ownership has been associated with both emotional and physical health benefits. however, owning pets may also pose health risks to immunocompromised patients through zoonotic transmission of disease. our initial impression was that there is a lack of any evidence base in information given by health care professionals regarding these risks. we therefore aimed to produce evidence-based guidelines addressing this issue. a pubmed search was undertaken and a variety of literature on zoonoses revi ...200616581294
co-detection of bartonella henselae, borrelia burgdorferi, and anaplasma phagocytophilum in ixodes pacificus ticks from california, usa.presence of bartonella dna was explored in 168 questing adult ixodes pacificus ticks from santa cruz county, california. bartonella henselae type i dna was amplified from 11 ticks (6.55%); previously, two (1.19%) were found to be infected with borrelia burgdorferi and five (2.98%) with anaplasma phagocytophilum. detection of b. henselae was not dependent on co-infection. the present study offers additional evidence that ixodes spp. ticks may act as hosts and possibly vectors for b. henselae.200616584332
molecular detection of microbes in nasal tissue of dogs with idiopathic lymphoplasmacytic rhinitis.lymphoplasmacytic rhinitis (lpr) is a common histologic finding in dogs with chronic nasal disease; however, potential etiologies of this disorder have not been examined. we investigated the hypothesis that specific microbes contribute to clinical disease in dogs with lpr. paraffin-embedded nasal biopsies were obtained from 19 dogs with lpr, 10 dogs with nasal neoplasia, and 10 dogs with nasal aspergillosis. nucleic acids were extracted from paraffin blocks, and real-time quantitative polymerase ...200616594580
rickettsial agents from parasitic dermanyssoidea (acari: mesostigmata).mites are often overlooked as vectors of pathogens, but have been shown to harbor and transmit rickettsial agents such as rickettsia akari and orientia tsutsugamushi. we screened dna extracts from 27 mites representing 25 species of dermanyssoids for rickettsial agents such as anaplasma, bartonella, rickettsia, and wolbachia by pcr amplification and sequencing. dna from anaplasma spp., a novel bartonella sp., spiroplasma sp., wolbachia sp., and an unclassified rickettsiales were detected in mite ...200616596351
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