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yersinia terminal ileitis: sonographic findings in eight patients.to determine the sonographic features of yersinia terminal ileitis, we analyzed the sonograms of eight patients with acute terminal ileitis. bacteriologic or serologic confirmation of yersinia enterocolitica infection was available in six patients. in the other two, the clinical course and radiologic findings were compatible with the diagnosis. radiographs and endoscopy showed edematous mucosa with small elevations in the terminal ileum in all the patients. sonograms showed thickening of the wal ...19912017961
[yersinia enterocolitica infection. apropos of a case with cervical localization].the authors report a case of cervical abscess due to yersinia enterocolitica. this is a currently exceptional localization of an enteropozoonosis which is little-known, but whose incidence is increasing throughout the world.19912018279
role of the yersinia outer membrane protein yada in adhesion to rabbit intestinal tissue and rabbit intestinal brush border membrane vesicles.the yersinia virulence plasmid confers on strains of yersinia pseudotuberculosis and y. enterocolitica an adhesive potential superior to the one encoded by the chromosome alone. we have evaluated the role of the plasmid-encoded outer membrane protein yada (formerly called yopl) in adhesion. insertional inactivation of the yada gene (formerly called yopa), which encodes yada, led to a reduction in the capacity of plasmid-carrying strains of y. pseudotuberculosis 0:iii and y. enterocolitica 0:9 to ...19912018636
yersinia enterocolitica in food hygiene.yersinia enterocolitica and yersinia enterocolitica-like bacteria constitute a fairly heterogenous group of bacteria which includes both well-established pathogens and a range of environmental strains which are ubiquitous in terrestrial and freshwater ecosystems. pathogenic significance in man is mainly associated with a few serogroups (o:3, o:9, o:8, o:5,27). the pathogenic serogroups show different geographical distributions. the development of isolation procedures which clearly differentiate ...19912018706
test detects/recovers harmful foodborne bacterium. 19912026519
risk of yersinia infection among butchers.the purpose of this study was to evaluate the risk of yersinia infection among butchers. serum samples were collected from 146 abattoir workers, stratified into 3 groups according to exposure to swine throats and intestines. 100 healthy blood donors were used as controls. antibodies against yersinia enterocolitica o:3 and o:9 and y. pseudotuberculosis i and iii were measured using elisa. symptoms associated with yersiniosis were recorded in a questionnaire. antibodies against y. enterocolitica o ...19912028228
[pork meat as a source of pathogenic yersinia enterocolitica].during a period of eight months, samples of carcasses, tonsils, tongues and rectal swabs were taken in four pig slaughter halls. porcine head meat was sampled in a meat products factory and samples of minced pork were taken in butcher's shops. pathogenic strains of yersinia enterocolitica (serological groups o:3, o:9 and o:5.27) were isolated from 36 (42 per cent) of 86 porcine tonsils, 8 (20 per cent) of 40 tongues, 17 (17 per cent) of 100 rectal swabs and from 4 (1 per cent) of 400 pork sample ...19912028456
suppurative cervical lymphadenitis after yersinia enterocolitica bacteremia.yersinia enterocolitica is increasingly recognized as a pathogen causing diverse complications. we have reported the case of a man with fever, abdominal tenderness, and y enterocolitica bacteremia. after antibiotic therapy, his condition improved initially, but later, suppurative cervical lymphadenitis developed. this suggests that the hematogenous spread of y enterocolitica to a distal lymphatic focus of infection is a possible complication of y enterocolitica bacteremia.19912035093
purification of lipopolysaccharide from strains of yersinia enterocolitica belonging to serogroups 03 and 09.lipopolysaccharide (lps) was purified from strains of yersinia enterocolitica belonging to serogroups 03 and 09, by three methods, and analysed by sds-page and silver staining for carbohydrate. sds-page of lps prepared from whole-cells by digestion with proteinase-k, produced profiles containing high molecular mass lps and a lower molecular mass region migrating as discrete bands. lps prepared from strains belonging to serogroup 03, using a hot-phenol procedure alone was found to contain cellula ...19912037238
[yersinia and campylobacter food poisoning]. 19912038688
evaluation of a kit system for identification of yersinia species of animal origin. 19912039797
aaem case report #21: hemifacial spasm: preoperative diagnosis and intraoperative management.a 75-year-old man developed progressive involuntary hemifacial spasm. electrophysiologic evidence of abnormal cross-transmission between neurons of the facial nerve was demonstrated. electrodiagnostic studies were used to confirm the diagnosis preoperatively and determine the adequacy of vascular decompression of the facial nerve intraoperatively.19912041543
yersinia species isolated from sheep with enterocolitis.in 40 submissions to the regional veterinary laboratory (rvl) wagga wagga from sheep in southern new south wales from 1981 to 1989, 53 isolates of yersinia sp were recovered from 45 sheep in 37 flocks. of 53 isolates, 26 were identified as y. pseudotuberculosis, 20 as y. enterocolitica, 5 as y. intermedia and 2 as y. frederiksenii. twelve isolates of y. pseudotuberculosis tested in the slide agglutination test all belonged to serotype iii. the 20 y. enterocolitica isolates were categorised bioch ...19912043083
sir frederick hobday memorial lecture. all wind and water: some progress in the study of equine gut motility. 19912044514
fumonisin mycotoxins and equine leukoencephalomalacia. 19912045319
the economics of spaying and neutering: market forces and owners' values affecting pet population control. 19912045338
cloning of chromosomal beta-lactamase genes from yersinia enterocolitica.two beta-lactamase genes present in the chromosome of yersinia enterocolitica have been cloned individually into the plasmid pacy184 and expressed in escherichia coli. the gene for broad-spectrum beta-lactamase i ('a') was cloned from a strain belonging to the o:3 serotype, and the gene for (cephalosporinase) beta-lactamase ii ('b') was cloned from a strain of the o:5b serotype. the properties of the beta-lactamases expressed in e. coli are similar to those previously described in y. enterocolit ...19912045777
[histiocytic necrotizing lymphadenitis; kikuchi's disease]. 19912046797
evasion of yersinia enterocolitica serotype 03 from complement-mediated killing.using isogenic strains of y. enterocolitica serotype 03 (isolate 75) differing in lps side chains (s and r), plasmid content (p+ and p-) or selective failure of yop1 expression (yop1-) we observed comparable c9 consumption via the alternative pathway by all strains. differences became apparent in the bactericidal assays in which the 75s p+ strain was resistant whereas the plasmid-negative s and r strains were killed. increased but submaximal resistance was observed with the 75r p+ and 75s yop1- ...19912049037
effects of high hydrostatic pressure on characteristics of pork slurries and inactivation of microorganisms associated with meat and meat products.pork slurries inoculated with various test microorganisms were prepared and subjected to high hydrostatic pressure at 1000 to 6000 atm for 10 min at 25 degrees c to examine for the pressure effects on characteristics of the slurries and the inactivation of the microorganisms associated with meat and meat products. pressure treatment at higher than 3000 atm caused coagulation and discoloration of the pork slurries. harder and more white coagulants were obtained by increasing the pressure. pressur ...19912049285
[yersinia enterocolitica--an infection with many camouflages]. 19912056816
[the characteristics of the course of yersiniosis against a background of rabies vaccination]. 19912063554
[yersinia in the soil of the fields irrigated with waste water from the swine breeding farms]. 19912065983
inhibition of plasmid-encoded adhesion of yersinia enterocolitica by non-immunoglobulin fraction of human milk.the inhibitory effect of human milk on plasmid-encoded adhesion of yersinia enterocolitica to rabbit ileal brush border membrane vesicles immobilized on polystyrene was evaluated. adhesion was reduced to about 50% after incubation of equal volumes of bacteria and undiluted human milk, but a significant reduction was also detectable after incubation with milk samples at a final 1:10 dilution. removal of immunoglobulins from human milk by means of immunoadsorption did not change the inhibitory eff ...19912069808
treatment of yersinia infection with tetracyclines. 19902076082
[microbiology and epidemiology of yersinia infections].eleven species are actually recognized within the genus yersinia of which three--y. pestis, y. pseudotuberculosis, and certain serovars of y. enterocolitica--are important infectious organisms for humans and warm-blooded animals. the causative agents of yersiniosis, y. pseudotuberculosis and y. enterocolitica, occur world-wide in areas of moderate and subtropical climate. asymptomatically infected warm-blooded animals, causing environmental contamination, are the most important factors for the e ...19902076900
[enteropathogenic yersinias: pathogenicity factors and new diagnostic methods].a limited number of serotypes of yersinia enterocolitica and y. pseudotuberculosis is enteropathogenic for humans. during the last decade molecular biology contributed significantly to the understanding of the pathogenicity of these enteric agents. all human pathogenic yersiniae harbour a 70-kilobase plasmid which is essential for virulence expression. presently, thirteen plasmid-encoded polypeptides have been identified. two of them have been identified as true outer membrane proteins and at le ...19902076901
[clinical aspects, diagnosis and therapy of yersinia enterocolitica infections].the predominant form (65% of all patients) of yersiniosis is mesenteric adenitis, resulting in enteritis, pseudo-appendicular syndromes, ileitis or colitis. the severity of this form depends to some degree on the age. extra-mesenteric forms (in 20-25%), focal infections by dissemination, the septic form and the lymphadenopathy syndrome may occur after or without previous enteritis. persistence of y. enterocolitica in lymphnodes and the galt is responsible for chronic and recurrent forms. seconda ...19902076902
antibodies to yersinia enterocolitica serotype 3 in autoimmune thyroid diseases.the prevalence of increased titres of antibodies to yersinia enterocolitica (serotype 3) has been studied in sera from patients with various thyroid diseases. in contrast to the low prevalences of the antibodies in healty subject (24.3%), titres (greater than 10) of anti-yersinia enterocolitica (anti-yersinia) were found more frequently in patients with thyroidal disorders, especially in graves' disease (70.0%). furthermore, high titres of the antibodies (greater than or equal to 160) were found ...19902083529
a permanent endoparasite of man. 1. the silent zoogleal/symplasm/l-form phase. 19902084495
[serological studies of human yersinia enterocolitica infections].sera of 1283 patients from hospitals and medical centers of wrocław were examined for y. enterocolitica antibodies. in 29% examined sera anti-y.enterocolitica antibodies of titer 1:40 and greater were noted. the agglutinins against serotype ia(3) were in 82.5%, against v(9) in 8.6% and against both serotypes mentioned above in 8.8%. in the group of sera positive for serotype ia 70.2% of sera gave positive reaction using antigen made from the humans strains and 9.1% using antigen prepared from sw ...19902084801
[role of yersinia infection in the pathogenesis of diffuse toxic goiter].antibodies to yersinia enterocolitica were tested in 87 patients with diffuse toxic goiter. they were detected in 32.1% of the patients and only 4.8% of the control group patients (p less than 0.01). the role of yersinia infection in breakdown of natural tolerance to autologous thyroid tissue is discussed.19902084934
[value of microbiologic studies for diagnosis of post-enteritis reactive arthritis].reactive arthritis may develop within a period of some days until upto 3 weeks after infections with yersinia enterocolitica, yersinia pseudotuberculosis, campylobacter jejuni/coli, shigella and salmonella. intestinal infections with klebsiella pneumoniae, clostridium perfringens, clostridium difficile, cryptosporidium, strongyloides stercoralis, taenia saginata and schistosoma mansoni are, in some cases, considered to be responsible for reactive arthritis. detection of pathogenic bacteria in fe ...19902085058
enteritis in sheep and goats due to yersinia enterocolitica infection.yersinia enterocolitica biotype 5, serotype 02,3 was isolated from the intestine of 38 sheep and 8 goats submitted to the laboratory for disease diagnosis. infected animals were usually young, had diarrhoea and were in poor condition or emaciated. a number were moribund or dead when submitted. characteristic microabscesses were demonstrated in the intestine of 5 of 38 sheep and 3 of 8 goats and no alternative cause of morbidity or mortality was established in these animals. of the 33 sheep and 5 ...19902085292
in vitro antimicrobial susceptibilities of yersinia enterocolitica and related species isolated from slaughtered pigs and pork products.the mics for and disk susceptibilities of 80 strains of yersiniae isolated from swine and pork products were determined. the most effective antimicrobial agents in the in vitro tests were the aminoglycosides, cephalosporins (cefotaxime, ceftazidime, and ceftriaxone), imipenem, ticarcillin-clavulanic acid, aztreonam, ciprofloxacin, norfloxacin, and trimethoprim-sulfamethoxazole. there was a high percentage of resistance among the strains to penicillinase-sensitive penicillins, erythromycin, clind ...19902088198
[a 3-year follow-up study of the incidence of campylobacteriosis in a pediatric population].the authors investigated the incidence of campylobacteriosis in the population of five paediatric health communities of the jihlava policlinic (5831 children) for a period of three years. a total of 2408 specimens faeces from 1501 subjects were examined. campylobacter jejuni was detected in 151 subjects (10.1%), salmonella sp. in 47 (3.1%), shigella sp. in 18 (1.2%), yersinia enterocolitica in 12 (0.8%) and enteropathogenic escherichia coli in five subjects (0.3%). the total incidence of campylo ...19902092913
department of health and human services. recombinant dna research; proposed actions under the guidelines.this notice sets forth proposed actions to be taken under the national institutes of health (nih) guidelines for research involving recombinant dna molecules. interested parties are invited to submit comments concerning these proposals. these proposals will be considered by the recombinant dna advisory committee (rac) at its meeting on october 16, 1990. after consideration of these proposals and comments by the rac, the director of the national institutes of health will issue decisions in accord ...19902093950
intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study.light and electron microscopy were used to analyze nine therapy-resistant and asymptomatic human periapical lesions, which were removed as block biopsies during surgical treatment of the affected teeth. the cases that required surgery represented about 10% of all of the cases which received endodontic treatment and root fillings during the period 1977 to 1984. these cases revealed periapical lesions when they were examined 4 to 10 yr after treatment. the biopsies were processed for correlated li ...19902094761
the roles of social work in psycho-biological research.social workers have an opportunity to utilize their skills in the arena of psychiatric research. traditional areas of social work functions, including working with patients, families, groups, complicated systems, and interdisciplinary teams, as advocate, therapist, mediator, and manager, are all necessary for the successful implementation of psychiatric research projects with human subjects. the repertoire of social work skills can enhance the successful operation of research involving bio-psych ...19902094962
[enteritis and erythema nodosum caused by yersinia enterocolitica serogroup 09]. 19902095273
[sweet's syndrome and yersinia enterocolitica infection]. 19902099706
delivery of the cholera toxin b subunit by using a recombinant yersinia enterocolitica strain as a live oral carrier.the gene ctxb encoding the cholera toxin b subunit was subcloned to design its production by yersinia enterocolitica. it was joined in two ways to yoph, a gene of the virulence plasmid pyv specific to this genus. this gene encodes one of the major yop proteins (yoph) secreted by bacteria incubated at 37 degrees c in a ca(2+)-deprived medium. in a first construction, an operon fusion was obtained between ctxb and yoph so that ct-b and a truncated yoph protein were produced. the recombinant ct-b f ...19902101483
secretor status and infection in patients with graves' disease.we have demonstrated that the inability to secrete the water soluble glycoprotein form of the abo blood group antigens into saliva is significantly more common in patients with graves' disease than control subjects (40% vs 27%: p less than 0.025) but not among those with hashimoto's thyroiditis or spontaneous primary atrophic hypothyroidism. non-secretion is associated with increased susceptibility to infection and to asymptomatic carriage of some microorganisms. although yersinia enterocolitica ...19902102769
yersinia enterocolitica and thyroid autoimmunity.the concordance rate of graves' disease in pairs of identical twins of 30 to 60% points to the influence of environmental factors, and infections have often been incriminated in the pathogenesis. more than 15 years ago we demonstrated an increased frequency of antibodies to yersinia enterocolitica (y. ent.) serotype 3, which later was confirmed by others, suggesting a link between infection with y. ent. and autoimmune thyroid diseases.19902102770
autoimmune thyroid diseases and enteropathogenic yersinia enterocolitica. 19902102771
antibody activity against lipopolysaccharides, lipid a and proteins from enterobacteriaceae in patients with chronic inflammatory liver diseases.these studies are concerned with detection of circulating antibodies against various defined enterobacterial antigens in patients with chronic inflammatory liver diseases such as chronic hepatitis type b (n = 46), chronic active hepatitis (cah) of autoimmune type (n = 10), alcoholic cirrhosis (n = 24) and primary biliary cirrhosis (pbc) (n = 24) as well as in healthy individuals (n = 39). anti-lps and anti-lipid a were determined by hemolytic and hemagglutination assay. immunoblot technique was ...19902102772
yersinia enterocolitica: mechanisms of microbial pathogenesis and pathophysiology of diarrhoea. 19902103396
[an analysis of the mechanisms of interpopulation interaction of yersinia with tetrahymena pyriformis infusorians at the cellular and subcellular levels].the mechanisms of interaction between the populations of yersinia and t. pyriformis have been analyzed on the cellular and subcellular levels. as shown in this investigation, yersinia, when phagocytized by t. pyriformis, may undergo morphological changes, remain unchanged and also multiply, destroying the host cell in the process.19902109915
listeria isolations from feces of patients with diarrhea and from healthy food handlers.a study was undertaken on the presence and frequency of listeria sp. in feces from 1,000 patients suffering from diarrheal diseases and from 2,000 healthy persons. furthermore, the feces of patients were examined for other well-documented enteropathogens such as campylobacter, salmonella, shigella, staphylococcus aureus, yersinia enterocolitica, protozoa and rotavirus as well as for organisms of questionable enteropathogenic potency such as fungi, i.e. candida. finally, in continuation of previo ...19902110118
the serological response of cattle immunized with yersinia enterocolitica o:9 or o:16 to yersinia and brucella abortus antigens in enzyme immunoassays.sera from calves immunized with yersinia enterocolitica serotypes o:9 or o:16 were tested by indirect enzyme linked immunosorbent assay (elisa) using lipopolysaccharide (lps) preparations from brucella abortus or y. enterocolitica o:9 or o:16 for their antibody content of the igg1 or igg2 subclasses. high igg1 responses were present with the three antigens in both groups although some individual variations between animals were noted. the igg2 responses were modest and in some cases not above bac ...19902111058
zinc binding and its trapping by allosteric transition in glucosamine-6-phosphate deaminase from escherichia coli.glucosamine-6-phosphate isomerase deaminase from escherichia coli, a typical allosteric enzyme, becomes less cooperative and 50% inhibited when treated with zinc. this metal cation behaving as a tight-bound and slow partial inhibitor. modification of a pair of vicinal reactive thiols with some sulfhydryl reagents mimics this effect. on the other hand, sulfhydryl reactivity disappears in the presence of saturating concentrations of zn2+, which does not modify the kinetics of s-methylated enzyme, ...19902111170
acp broadsheet 124: may 1990. examination of faeces for bacterial pathogens. 19902115050
role of enterobacteria and hla-b27 in spondyloarthropathies: studies with transgenic mice.several mhc (major histocompatibility complex) genes are associated with increased incidence of disease. the strongest association is between the class i gene, hla-b27, and ankylosing spondylitis (as). hla-b27 is also highly associated with reiter's syndrome. as not all subjects with hla-b27 develop as or reiter's syndrome, environmental factors may have a key role in the pathogenesis of these arthritic diseases. several studies have implicated klebsiella in the development of as, whereas reiter ...19902115764
growth of pathogenic yersinia enterocolitica strains in minced meat with and without protective gas with consideration of the competitive background flora.the growth of two pathogenic yersinia enterocolitica strains (o:3, o:9 at 1 degree c, 4 degrees c, 10 degrees c and 15 degrees c) in minced meat produced under sterile conditions and in minced meat with a normal background flora was investigated. in addition, the influence of treatment with a protective gas (20% co2, 80% o2) was tested. in minced meat produced under sterile conditions and incubated under normal atmospheric conditions, y. enterocolitica increased in numbers by only 1 log at 1 deg ...19902118793
resonance raman study on mutant cytochrome p-450 obtained by site-directed mutagenesis.resonance raman spectra were observed for the threonine-301 to serine or valine mutant as well as the wild type of rabbit liver microsomal cytochrome p-450 [laurate(omega-1)-hydroxylase] (p-450(omega-1], which were prepared through site-directed mutagenesis. the high-spin marker resonance raman (rr) bands became similarly stronger for all the p-450s examined in the oxidized form upon addition of laurate, and the rr spectra in the higher frequency region of the oxidized, reduced and co-adduct for ...19902119227
yersinia enterocolitica infections during the holidays in black families--georgia. 19902122217
acidity and intestinal bacteria: an in-vitro assessment of the bactericidal activity of hydrochloric acid on intestinal pathogens.the bactericidal activity of hydrochloric acid (hcl) on different intestinal bacteria was evaluated at various ph values and time intervals. the effect of hcl was highest at ph 2 and ph 3 on strains of campylobacter jejuni, aeromonas hydrophila, plesiomonas shigelloides, yersinia enterocolitica, salmonella spp. and shigella spp., producing complete killing after 60 min incubation. however, at higher ph values (ph 4 and above), these organisms not only survived but they also showed evidence of ra ...19902127996
influence of recombinant interferon-gamma qn the expression of mhc class i and class ii antigens on four human colonic carcinoma cell lines.the occurrence of mhc class i and class ii antigens on four human colonic carcinoma cell lines and the effect of recombinant interferon-gamma (rifng) on the expression of these antigens was investigated by immunofluorescent flow cytometry. the concentration of rifng which resulted in the largest increase in expression of class i and class ii antigens was determined. changes in the amount of mhc antigen on the membrane were indicated by a shift in the mean fluorescence intensity (mfi) of the cell ...19902129402
secretion of yop proteins by yersiniae.upon incubation at 37 degrees c in the absence of ca2+ ions, pathogenic strains of the genus yersinia cease growing and produce large amounts of a series of plasmid-encoded proteins involved in pathogenicity. these proteins, called yops (for yersinia outer membrane proteins), are detected in both the outer membrane fraction and the culture supernatant. we present here the nucleotide sequence of genes yop20 and yop25 from yersinia enterocolitica o:9. protein yop25 is very similar to yope, the cor ...19902129533
recombinant erythropoietin reverses polymorphonuclear granulocyte dysfunction in iron-overloaded dialysis patients.iron overload increases the risk of bacterial infection in dialysis patients, partly by impairing functions of the polymorphonuclear granulocytes (pmns). pmn defence was studied sequentially in haemodialysis patients with transfusional haemosiderosis, treated for 6 +/- 1.5 months (n = 8) to 13 +/- 1.7 months (n = 4) with recombinant human erythropoietin (rhuepo). over this period, signs of iron overload (increased serum ferritin and serum iron) improved, and stainable iron disappeared in pmns. s ...19902130296
[isolation of yersinia enterocolitica o3 by an enrichment procedure at room temperature]. 19902131974
[natural anaplasma marginale infection in 2 cattle herds with different levels of boophilus microplus tick infestation].the dynamics of natural infections by anaplasma marginale was studied in two adjacent dairy farms with different levels of boophilus microplus infestation. the farms were located in the enzootic area of bovine anaplasmosis of the northwest of argentina. the study was carried out in 35 calves from birth in march-august 1985 to march 1986. the infection rate by a. marginale was evaluated by the observation of blood films and by determination of specific antibodies. the degree of infestation by b. ...19902132783
equine culicoides hypersensitivity in florida: biting midges aspirated from horses.nine species of culicoides were aspirated from horses in florida during the second phase of a study of equine culicoides hypersensitivity (chs). approximately 90% of the 2933 midges were culicoides insignis lutz, 4% were c.stellifer (coquillett), 3% were c.niger root and hoffman, 2% were c.alachua jamnback and wirth and the remaining 1% included c.venustus hoffman, c.scanloni wirth and hubert, c.lahillei iches (= c.debilipalpis lutz), c.pusillus lutz, and c.edeni wirth and blanton. culicoides we ...19902133005
[mixed culture of enterococcus faecium sf 68 with pathogenic intestinal bacterial strains].an antagonistic activity of enterococcus faecium sf 68 towards plesiomonas shigelloides, aeromonas sp., enteropathogenic escherichia coli and yersinia enterocolitica has been studied and demonstrated.19902133323
a new prepatory method of thermically inactivated leptospira patoc antigen for rapid slide agglutination used as serosurvey test for human leptospiroses.830 sera from leptospirosis-suspect patients and 133 sera from investigations were analysed by the new test--rapid slide agglutination with patoc antigen, inactivated at 60 degrees c for 15 minutes, in parallel with two standard tests--cft with merthiolated patoc antigen and mar with 17-23 live pathogenic antigens. the new serotest proved efficient for selecting the cases of disease or leptospira infection, acute or recent form, not older than 2 years, both by its sensitivity and specificity and ...19902134149
[electron microscopic observation for periodontal tissue regeneration after implantation of atelocollagen membrane].the purpose of the present study was to clarify wound healing process of periodontal tissue following experimental flap surgery, when applied the guided tissue regeneration (gtr) technique using a cross-linked atelocollagen membrane (am). mucoperiosteal flaps were made on the palatal gingiva of maxillary first molars of rats. the cementum was removed by curettage in order to expose the dentin surface. an am was implanted into the site of dissection in the experimental group, while the control gr ...19902134270
[the sensitivity of yersinia to bacteriophage mu].various representatives of the genus yersinia were found to differ in their sensitivity to the lytic action of bacteriophage mu cts62, which could serve as an auxiliary test for the differentiation of y. pestis and y. pseudotuberculosis. among the strains under study, the causative agents of plague (34 strains) were sensitive to phage mu cts62, while the causative agents of enteric yersiniosis (42 strains) and pseudotuberculosis (73 strains), except 3 strains with the properties of y. pestis, we ...19902146845
delay of puberty and impairment of growth in female rats given a non competitive antagonist of nmda receptors.reportedly, excitatory amino acids are involved in the control of gonadotropin secretion of rats and non-human primates. the aim of this study was to investigate the effect of chronic blockade of nmda (n-methyl-d-aspartic acid) receptors by the non competitive receptor antagonist mk-801 on gonadotropin secretion and the onset of puberty in female rats. moreover, since in humans alterations of the timing of puberty frequently coexist with disturbances of body growth, suggesting a common etiology ...19902147050
[systemic yersinia infection of cutaneous origin]. 19902147504
[acute appendicitis in patients with yersinia infection].the article is the result of the joint work of a surgeon and a specialist in infectious diseases and is based on the results of treatment of 307 patients with the abdominal form of yersinia infection acute appendicitis was diagnosed in 73 (23.8%) of them. the abdominal form of yersinia infection is encountered frequently among children. the authors analyse the clinical picture of acute appendicitis in patients with yersinia infection, the time between the onset of the disease and the patient's c ...19902149395
[yersinia in the water of wells near an area of irrigation with the effluents from a swine-breeding farm complex].water taken from wells situated in the zone of irrigation with sewage water from a swine-breeding complex was studied. in 25.4% of samples y. enterocolitica and y. intermedia were detected. in winter and spring months twice as many strains were isolated in comparison with the number of strains isolated in summer and autumn. no yersinia were isolated from control water samples taken from wells remote from the irrigation zone. the epidemiological relationship between a case of yersinia infection a ...19902150143
[occurrence of yersinia enterocolitica, particularly serovar 03, biovar 4 in pork and beef].the paper deals with the incidence of yersiniae in pork and beef, focused in particular on the prevalence of yersinia enterocolitica, serovar 3, biovar 4. the author uses for detection the method of cold shock, alkalization of the sample, taking advantage of the tolerance of yersinias of diluted alkalis and suppressing the contaminating microflora, and the the selective medium cin agar with a locally manufactured supplement. cultivation was either direct or after propagation in phosphate buffer ...19902150608
[the spread of the causative agent of yersiniosis in the regions of sochi and adjacent areas].yersinia enterocolitica sensu stricto, y. kristensenii, y. aldovae and y. intermedia strains were for the first time isolated in the sochi districts from people, synanthropic rodents and washings from vegetables. in the adjacent alpine and subalpine areas isolates were obtained from wild rodents. 86.7% of the isolated strains were assigned to the known antigenic variants; o16; o5; o6.31; o7.8 and o6.30 serovars were predominant. all the strains isolated from people belonged to biovar 1 and o5; o ...19902150687
evidence for regulation of the autoimmune anti-erythrocyte response by idiotype-specific suppressor t cells in nzb mice.the present study demonstrates that specific cd8+, cd4- suppressor t cells (ts) actively regulate the autoimmune anti-mouse red blood cell (mrbc) antibody response in spleen cell populations of young, coombs-negative nzb mice. these ts appear to bind a monoclonal nzb autoantibody (g-8 mab) to unmodified mrbc which expresses a dominant idiotype (id) in the spontaneous anti-mrbc autoantibody response of nzb mice. treatment of normally nonauto-responsive spleen cells from young nzb mice with the g- ...19902150924
evidence for regulation of the autoimmune anti-erythrocyte response by idiotype-specific suppressor t cells in nzb mice.the present study demonstrates that specific cd8+, cd4- suppressor t cells (ts) actively regulate the autoimmune anti-mouse red blood cell (mrbc) antibody response in spleen cell populations of young, coombs-negative nzb mice. these ts appear to bind a monoclonal nzb autoantibody (g-8 mab) to unmodified mrbc which expresses a dominant idiotype (id) in the spontaneous anti-mrbc autoantibody response of nzb mice. treatment of normally nonauto-responsive spleen cells from young nzb mice with the g- ...19902150924
[yersinia in plants].plants collected on the plots of land formerly irrigated with sewage water from a swine breeding complex (in sewage and the soil yersinia had been earlier detected) were studied. in 4.9% of plant samples yersinia were detected (y. enterocolitica and y. intermedia). the isolated strains were biochemically and serologically identical to those detected in sewage and the soil. no yersinia were detected in washings from the surface of these plants, as well as in the study of control plants. these fac ...19902151495
simultaneous primary infection with hiv and cmv leading to severe pancytopenia, hepatitis, nephritis, perimyocarditis, myositis, and alopecia totalis.simultaneous primary infection with hiv and cmv in an 18-year-old woman led to an acute cytotoxic reaction, manifesting as pancytopenia, hepatitis, nephritis, perimyocarditis, and myositis. within 14 days parameters indicating acute cell damage reverted to normal. two weeks later transient alopecia totalis developed. initially, hiv-antigen but no hiv antibodies were present. within 3 weeks hiv-igg antibodies appeared while hiv antigen disappeared. anti-cmv-igm was clearly and anti-cmv-igg questi ...19902156107
the lcre gene is part of an operon in the lcr region of yersinia enterocolitica o:3.the low-calcium response (lcr) region of the virulence plasmid of yersinia pestis, yersinia pseudotuberculosis, and yersinia enterocolitica has been associated with calcium-dependent growth of bacteria. mutations in the previously identified lcre locus within the lcr region lack the repressor control of production of the lcr specific proteins, yersinia outer membrane proteins (yops) and v and w antigens. we sequenced a 3.3-kilobase-pair bamhi-clai fragment of the lcre locus of pyvo3, the virulen ...19902160939
serological cross-reactions against yersinia enterocolitica in patients infected with other arthritis-associated microbes.in order to investigate arthritis-triggering, serologically cross-reactive antigens, sera from patients infected with arthritis-associated microbes, salmonellae, chlamydia trachomatis, and sindbis-like alphavirus, were reacted against sds-page separated and immunoblotted yersinia enterocolitica 0:3 antigens. these sera reacted with yersinia to the same extent as did the control sera taken from patients with streptococcal, staphylococcal and bordetella pertussis infections or from healthy blood d ...19902161307
antagonizing effects of phorbol 12-myristate 13-acetate on hormonally stimulated gluconeogenesis in isolated rat hepatocytes involve activity changes of pyruvate kinase.the tumor-promoting phorbol ester phorbol 12-myristate 13-acetate partially neutralized the stimulatory effects of epinephrine (alpha 1-adrenergic actions), glucagon, and dibutyryl-camp on gluconeogenesis in isolated hepatocytes of fasted rats, when lactate or dihydroxyacetone was used as the substrate. by constructing metabolic crossover plots and by comparing rates of lactate production from dihydroxyacetone with k0.5 values of extracted pyruvate kinase for phosphoenolpyruvate, we obtained evi ...19902168154
kinetics of the reaction of prostaglandin h synthase compound ii with ascorbic acid.the reduction of prostaglandin h synthase compound ii by ascorbic acid in the presence of diethyldithiocarbamate was studied in 0.1 m phosphate buffer (ph 8.0) at 4.0 +/- 0.5 degrees c, by rapid scan spectrometry and transient state kinetics. a saturation effect and nonzero intercept were observed in the plot of pseudo-first-order rate constant versus ascorbic acid concentration. the saturation behavior suggests formation of a complex between prostaglandin h synthase compound ii and ascorbic aci ...19902168160
long-term inhibition of dna synthesis and the persistence of trapped topoisomerase ii complexes in determining the toxicity of the antitumor dna intercalators mamsa and mitoxantrone.the cytotoxic actions of several classes of antitumor dna intercalators are thought to result from some disturbance to dna metabolism following trapping of the nuclear enzyme dna topoisomerase ii as a covalent complex on dna. here we have studied topoisomerase ii trapping and dna synthesis patterns in relation to the acute cytotoxic actions of 4'-(9-acridinylamino)methanesulfon-m-anisidide (mamsa) or mitoxantrone on sv40 transformed human fibroblasts. these two dna intercalators differed signifi ...19902168281
accuracy and precision of computer-simulated tissue temperatures in individual human intracranial tumours treated with interstitial hyperthermia.accurate knowledge of tissue temperature is necessary for effective delivery of clinical hyperthermia in the treatment of malignant tumours. this report compares computer-predicted versus measured intratumoral temperatures in 11 human subjects with intracranial tumours, treated with a conceptually simple 'conductive' interstitial hyperthermia system. interstitial hyperthermia was achieved by the use of parallel arrays of implanted, electrically heated catheters. the tissue was warmed by thermal ...19902168460
determination of bhv1 specific immune reactivity in naturally infected and vaccinated animals by lymphocyte proliferation assays.the in vitro bhv1-specific lymphocyte stimulation assay was used to investigate immune reactivity of cattle after natural infection or vaccination with bhv1. proliferative responses to live virus were shown in tests with peripheral blood lymphocytes of seropositive field virus-infected animals and of vaccinated animals. nineteen out of 36 seropositive field virus-infected animals did not show in vitro responses. nine out of 12 animals showed, at least transient, responsiveness after vaccination. ...19902168602
effect of particle size and temperature on the conformation and physiological behavior of apolipoprotein e bound to model lipoprotein particles.the effect of particle size and structural order/disorder of the lipid domain on the conformation and physiological behavior of lipid-associated apolipoprotein e (apoe) was evaluated. circular dichroic (cd) spectra of apoe bound to large (lme) and small (sme) microemulsion particles, composed of dimyristoylphosphatidylcholine (dmpc) and cholesteryl oleate (co), and to dmpc disks revealed that at 4 degrees c, where all of the lipid constituents were in an ordered state, apoe bound to lme displaye ...19902168737
import into mitochondria of precursors containing hydrophobic passenger proteins: pretreatment of precursors with urea inhibits import.we have studied the import into isolated yeast mitochondria of three hydrophobic passenger proteins attached to the n-terminal cleavable presequence of mitochondrial atpase subunit 9 from neurospora crassa. one natural precursor (pn9) contained n. crassa subunit 9; two chimaeric precursors, n9l/y8-1 and n9l/y9-2, respectively contained yeast mitochondrial atpase subunits 8 and 9. in the absence of urea, pn9 and n9l/y8-1 are imported efficiently but n9l/y9-2 is not imported. after pretreatment of ...19902168755
[yersinia infection and hemoglobin disorder. apropos of 4 cases].the authors report 4 cases of yersinia enterocolitica (ye) infection in patients with hemoglobin disorders (3 beta thalassemia major, 1 sickle cell anemia). patients with hemoglobin disorders are particularly vulnerable to ye infection. predisposing factors consist mainly of iron overload and deferoxamine therapy; superinfection with hiv may be an additional factor. the diagnosis is performed by identifying patients at risk and by bacteriologic tests (blood and stool cultures, antibody titers). ...19902170907
outer membrane permeability and porin proteins of yersinia enterocolitica.in intact cells of yersinia enterocolitica, loss of the yompf protein reduced the outer membrane permeability parameters for two beta-lactam compounds, cephaloridine and nitrocefin. additional loss of the outer membrane yompc protein resulted in further reductions in the permeability parameters. we conclude that the outer membrane proteins yompc and yompf of y. enterocolitica are porin proteins.19902172075
biphasic effects of polarizing current on adenosine-sensitive generation of cyclic amp in rat cerebral cortex.cyclic amp accumulation elicited by adenosine was investigated in cortical slices of rats following the application of a weak anodal direct current (anodal polarization) to the unilateral sensorimotor cortex. anodal polarization with 3.0 microa for 30 min caused an increase in the adenosine-elicited accumulation of cyclic amp in the polarized cortex, in which case the increase in the polarized cortical region was highlighted by repeated applications of the currents. polarization with 0.3 microa ...19902173816
[quantitative and qualitative studies of yersinia species in the waste water of a purification plant].during a long term investigation (october 1986 october 1987) yersinia spp. were isolated from sewage in 5 different stages of a sewage treatment plant in kiel (bülk), schleswig-holstein, frg. methods applied were mpn-procedure, cold enrichment in cin-broth and finally incubation on cin-agar (cefsulodin-irgasan-novobiocin). yersinia enterocolitica were specified according to their serotypes (o:3/o:9) and biotypes. results (median/100 ml): sewage influent 90,000 kbe (log 4.95), aerated grit remova ...19902180421
yersinia enterocolitica and related species isolated in the pesaro and urbino area (italy) from 1981 to 1986.a total of 23 strains of yersinias, y. enterocolitica (17), y. frederiksenii (5) and y. intermedia (1) characterized according to bio-serogroup and phage type, were isolated from human, animal and environmental samples during a 5-year period. it appears that in the pesaro-urbino area yersinia spp. are infrequent and the strains of y. enterocolitica belong to environmental and rarely to human pathogenic bioserogroups.19902180887
evaluation of different techniques for detection of virulence in yersinia enterocolitica. 19902185270
a family of positive regulators related to the pseudomonas putida tol plasmid xyls and the escherichia coli arac activators.the xyls family consists of a least 8 different transcriptional regulators. six of these proteins are positive regulators for the catabolism of carbon sources (benzoate and sugars) in escherichia coli, pseudomonas putida and erwinia carotovora, and two of them are involved in pathogenesis in escherichia coli and yersinia enterocolitica. based on protein alignments, the members of this family exhibit a long stretch of homology at the c-terminal end. the regulators involved in the catabolism of ca ...19902186376
effect of acute yersinia enterocolitica infection on small intestinal ultrastructure.the purpose of this study was to assess the jejunal and ileal brush border injury caused by yersinia enterocolitica and to correlate these alterations with functional abnormalities. weanling rabbits infected with 10(10) organisms of a human pathogenic y. enterocolitica strain were compared with control and pair-fed, sham-treated animals. on day 6, infection resulted in a diffuse decrease in brush border enzyme activities in the small intestine and villus atrophy and crypt hyperplasia in the ileu ...19902186949
reversible leukopenia related to ciprofloxacin therapy.in general, ciprofloxacin is well tolerated. the most common adverse effects are nausea, vomiting, diarrhea, dizziness, nervousness, and rash. reversible leukopenia can be associated with the use of oral ciprofloxacin, as shown by our case. leukopenia should be watched for as the use of this drug increases.19902188377
psittacosis presenting with erythema-marginatum-like lesions--a case report and a historical review.a 14-year-old boy with psittacosis who developed skin lesions consistent with erythema marginatum is reported. this association has not been described previously.19902194716
genetic evidence that the yopa gene-encoded yersinia outer membrane protein yop1 mediates inhibition of the anti-invasive effect of interferon.hep-2 cell monolayers were challenged with genetic variants of yersinia pseudotuberculosis ypiii(pib1) and yersinia enterocolitica w22708(pyl4). both strains were represented by (i) variants harboring the 70-kilobase virulence plasmid, (ii) their isogenic plasmid-cured derivatives, and (iii) two transposon mutants constructed by insertional inactivation of the plasmid genes encoding outer membrane protein yop1 and ca2+ dependency in strains ypiii(pib1) and w22708(pyl4). when the hep-2 cells were ...19902194966
serum and tissue cage fluid concentrations of ciprofloxacin after oral administration of the drug to healthy dogs.ciprofloxacin, a fluoroquinolone antimicrobial agent, was administered orally to 4 healthy dogs at dosage of approximately 11 and 23 mg/kg of body weight, every 12 hours for 4 days, with a 4-week interval between dosing regimens. serum and tissue cage fluid (tcf) concentrations of ciprofloxacin were measured after the first and seventh dose of each dosing regimen. the peak concentration was greatest in the serum after multiple doses of 23 mg/kg (mean +/- sem; 5.68 +/- 0.54 micrograms/ml) and lea ...19902195927
aluminum, a neurotoxin which affects diverse metabolic reactions.experimental evidence is summarized to support the hypothesis that chronic exposure to low levels of aluminum may lead to neurological disorders. these disorders result from defective phosphorylation--dephosphorylation reactions, reduced glucose utilization and site-specific damage inflicted by free radicals produced by altered iron metabolism. the brain is a highly compartmentalized organ. therefore, a co-localization of critical mass of metabolic errors rather than a single event may be essent ...19902198876
comparison of restriction endonuclease analysis and phenotypic typing methods for differentiation of yersinia enterocolitica isolates.restriction endonuclease analysis of chromosomal dna (reac) was used to study polymorphism in restriction fragment patterns among yersinia enterocolitica isolates belonging to serogroups o3, o5,27, o8, o9, o13, and o21. using the enzyme haeiii and electrophoresis on thin (0.75-mm) vertical 5% polyacrylamide gels, we were able to distinguish at least 22 dna fragment patterns among the 72 strains examined. the method showed the greatest discriminatory power with regard to serogroup o8, within whic ...19902199484
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