| defining falciparum-malaria-attributable severe febrile illness in moderate-to-high transmission settings on the basis of plasma pfhrp2 concentration. | in malaria-endemic settings, asymptomatic parasitemia complicates the diagnosis of malaria. histidine-rich protein 2 (hrp2) is produced by plasmodium falciparum, and its plasma concentration reflects the total body parasite burden. we aimed to define the malaria-attributable fraction of severe febrile illness, using the distributions of plasma p. falciparum hrp2 (pfhrp2) concentrations from parasitemic children with different clinical presentations. | 2012 | 23136222 |
| defining falciparum-malaria-attributable severe febrile illness in moderate-to-high transmission settings on the basis of plasma pfhrp2 concentration. | in malaria-endemic settings, asymptomatic parasitemia complicates the diagnosis of malaria. histidine-rich protein 2 (hrp2) is produced by plasmodium falciparum, and its plasma concentration reflects the total body parasite burden. we aimed to define the malaria-attributable fraction of severe febrile illness, using the distributions of plasma p. falciparum hrp2 (pfhrp2) concentrations from parasitemic children with different clinical presentations. | 2012 | 23136222 |
| multidrug-resistance and toxic metal tolerance of medically important bacteria isolated from an aquaculture system. | the use of antimicrobials and toxic metals should be considered carefully in aquaculture and surrounding environments. we aimed to evaluate medically relevant bacteria in an aquaculture system and their susceptibility to antimicrobials and toxic metals. selective cultures for enterobacteria (ent), non-fermenting gram-negative rods (nfr) and gram-positive cocci (gpc) were obtained from water samples collected in two different year seasons. the isolated bacteria were biochemically identified and a ... | 2012 | 22972388 |
| bacterial contamination of dental unit waterlines. | safety of patients and dental personnel requires the appropriate microbiological water quality in dental units. during treatment, patients and dental workers are exposed both to direct contact with bacteria-contaminated water in the form of splatter and with contaminated water aerosol emitted during work by unit handpieces, including rotating and ultrasonic instruments. the aim of the study was to determine the qualitative and quantitative contamination of water in dental unit reservoirs with ae ... | 2012 | 22899458 |
| bacterial contamination of dental unit waterlines. | safety of patients and dental personnel requires the appropriate microbiological water quality in dental units. during treatment, patients and dental workers are exposed both to direct contact with bacteria-contaminated water in the form of splatter and with contaminated water aerosol emitted during work by unit handpieces, including rotating and ultrasonic instruments. the aim of the study was to determine the qualitative and quantitative contamination of water in dental unit reservoirs with ae ... | 2012 | 22899458 |
| complete sequence of a novel 178-kilobase plasmid carrying bla(ndm-1) in a providencia stuartii strain isolated in afghanistan. | in response to global concerns over the spread of the new delhi metallo-β-lactamase gene 1, bla(ndm-1), a monthly surveillance program was initiated in september 2010. all carbapenem-resistant gram-negative strains forwarded to our facility are screened for this gene. to date, 321 carbapenem-resistant isolates, encompassing 11 bacterial species, have been tested. in february 2011, two strains of providencia stuartii, submitted from a military hospital in afghanistan, tested positive for bla(ndm- ... | 2012 | 22290972 |
| biodegradation of di-n-butyl phthalate by a newly isolated halotolerant sphingobium sp. | a gram-negative strain (tj) capable of growing aerobically on mixed phthalate esters (paes) as the sole carbon and energy source was isolated from the haihe estuary, tianjin, china. it was identified as belonging to the sphingobium genus on the basis of morphological and physiological characteristics and 16s rrna and gyrb gene sequencing. the batch tests for biodegradation of di-n-butyl phthalate (dbp) by the sphingobium sp. tj showed that the optimum conditions were 30 °c, ph 7.0, and the absen ... | 2013 | 24336064 |
| prevent infection linked to the dialysis water in a hemodialysis center in fez city (morocco). | water treatment systems are a critical variable in dialysis therapy. rigorous control of hemodialysis water quality is particularly important in order to guarantee a better quality of life of the hemodialysis patients. the objective of the study was to evaluate the chemical, microbiological quality and antimicrobial resistance of bacteria isolated from water and dialysate in a public hd center. | 2013 | 24839530 |
| biocatalysis for biobased chemicals. | the design and development of greener processes that are safe and friendly is an irreversible trend that is driven by sustainable and economic issues. the use of biocatalysis as part of a manufacturing process fits well in this trend as enzymes are themselves biodegradable, require mild conditions to work and are highly specific and well suited to carry out complex reactions in a simple way. the growth of computational capabilities in the last decades has allowed biocatalysis to develop sophisti ... | 2013 | 24970192 |
| structure and evolution of chlorate reduction composite transposons. | the genes for chlorate reduction in six bacterial strains were analyzed in order to gain insight into the metabolism. a newly isolated chlorate-reducing bacterium (shewanella algae acdc) and three previously isolated strains (ideonella dechloratans, pseudomonas sp. strain pk, and dechloromarinus chlorophilus nss) were genome sequenced and compared to published sequences (alicycliphilus denitrificans bc plasmid palide01 and pseudomonas chloritidismutans aw-1). de novo assembly of genomes failed t ... | 2013 | 23919996 |
| genome analysis and physiological comparison of alicycliphilus denitrificans strains bc and k601(t.). | the genomes of the betaproteobacteria alicycliphilus denitrificans strains bc and k601(t) have been sequenced to get insight into the physiology of the two strains. strain bc degrades benzene with chlorate as electron acceptor. the cyclohexanol-degrading denitrifying strain k601(t) is not able to use chlorate as electron acceptor, while strain bc cannot degrade cyclohexanol. the 16s rrna sequences of strains bc and k601(t) are identical and the fatty acid methyl ester patterns of the strains are ... | 2013 | 23825601 |
| multilaboratory study of the biomic automated well-reading instrument versus microscan walkaway for reading microscan antimicrobial susceptibility and identification panels. | this study compared the biomic automated well reader results to the microscan walkaway results for reading microscan antimicrobial susceptibility and identification panels at four different sites. routine fresh clinical isolates and quality control (qc) organisms were tested at each study site. a total of 46,176 microscan panel drug-organism combinations were read. the biomic category agreement for 3,117 gram-negative bacteria was 98.4%, with 1.4% minor and 0.2% major discrepancies. the biomic c ... | 2013 | 23486713 |
| prevalence of antibacterial resistant bacterial contaminants from mobile phones of hospital inpatients. | mobile phones contaminated with bacteria may act as fomites. antibiotic resistant bacterial contamination of mobile phones of inpatients was studied. one hundred and six samples were collected from mobile phones of patients admitted in various hospitals in jazan province of saudi arabia. eighty-nine (83.9%) out of 106 mobile phones were found to be contaminated with bacteria. fifty-two (49.0%) coagulase-negative staphylococcus, 12 (11.3%) staphylococcus aureus, 7 (6.6%) enterobacter cloacae, 3 ( ... | 2014 | 25292217 |
| selection and characterization of biofuel-producing environmental bacteria isolated from vegetable oil-rich wastes. | fossil fuels are consumed so rapidly that it is expected that the planet resources will be soon exhausted. therefore, it is imperative to develop alternative and inexpensive new technologies to produce sustainable fuels, for example biodiesel. in addition to hydrolytic and esterification reactions, lipases are capable of performing transesterification reactions useful for the production of biodiesel. however selection of the lipases capable of performing transesterification reactions is not easy ... | 2014 | 25099150 |
| rapid identification of stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization. | the objective of this study was to develop a novel peptide nucleic acid (pna) probe for stenotrophomonas maltophilia identification by fluorescence in situ hybridization (fish). the probe was evaluated using 33 human and veterinary clinical s. maltophilia isolates and 45 reference strains representing common bacterial species in the respiratory tract. the probe displayed 100% sensitivity and 100% specificity on pure cultures and allowed detection in sputum from cystic fibrosis patients. the dete ... | 2014 | 25356348 |
| antimicrobial peptides from fish. | antimicrobial peptides (amps) are found widely distributed through nature, and participate in the innate host defense of each species. fish are a great source of these peptides, as they express all of the major classes of amps, including defensins, cathelicidins, hepcidins, histone-derived peptides, and a fish-specific class of the cecropin family, called piscidins. as with other species, the fish peptides exhibit broad-spectrum antimicrobial activity, killing both fish and human pathogens. they ... | 2014 | 24594555 |
| recent advances in lipase-mediated preparation of pharmaceuticals and their intermediates. | biocatalysis offers an alternative approach to conventional chemical processes for the production of single-isomer chiral drugs. lipases are one of the most used enzymes in the synthesis of enantiomerically pure intermediates. the use of this type of enzyme is mainly due to the characteristics of their regio-, chemo- and enantioselectivity in the resolution process of racemates, without the use of cofactors. moreover, this class of enzymes has generally excellent stability in the presence of org ... | 2015 | 26690428 |
| comparison of serum procalcitonin in respiratory infections and bloodstream infections. | this study observed the relationship between procalcitonin (pct) and results of sputum culture, the relationship between pct and results of blood culture to evaluate and compare the value of pct in respiratory and bloodstream infections. | 2015 | 26885109 |
| genotypic and phenotypic heterogeneity in alicyclobacillus acidoterrestris: a contribution to species characterization. | alicyclobacillus acidoterrestris is the main cause of most spoilage problems in fruit juices and acidic products. since soil borne species often contaminate fruit juices and do not need strict extreme requirements for survival, it is a great concern to investigate whether and how soil species could evolve from their ecological niches in microbial community to new environments as fruit juices. in this study, 23 isolates of thermo-acidophilic, spore-forming bacteria from soil were characterized by ... | 2015 | 26484547 |
| comparative genomics of non-pseudomonal bacterial species colonising paediatric cystic fibrosis patients. | the genetic disorder cystic fibrosis is a life-limiting condition affecting ∼70,000 people worldwide. targeted, early, treatment of the dominant infecting species, pseudomonas aeruginosa, has improved patient outcomes; however, there is concern that other species are now stepping in to take its place. in addition, the necessarily long-term antibiotic therapy received by these patients may be providing a suitable environment for the emergence of antibiotic resistance. to investigate these issues, ... | 2015 | 26401445 |
| evaluation of aerial microbial pollutants in al-haram al-nabawi during pilgrimage of 2013. | al-madinah al-munawwarah is the second holiest site in islam. the possibility of new emerging microbes is valid due to the increased number of pilgrims. the objectives of the current study were to estimate the numbers of fungi and bacteria inside and outside al-haram al-nabawi and to find whether new bacterial and fungal species have emerged compared to previous studies. air samples were collected twice a day from 12 spots and four directions during the pilgrim year of 2013 for four consecutive ... | 2015 | 28053593 |
| evaluation of aerial microbial pollutants in al-haram al-nabawi during pilgrimage of 2013. | al-madinah al-munawwarah is the second holiest site in islam. the possibility of new emerging microbes is valid due to the increased number of pilgrims. the objectives of the current study were to estimate the numbers of fungi and bacteria inside and outside al-haram al-nabawi and to find whether new bacterial and fungal species have emerged compared to previous studies. air samples were collected twice a day from 12 spots and four directions during the pilgrim year of 2013 for four consecutive ... | 2015 | 28053593 |
| aerobic cyanide degradation by bacterial isolates from cassava factory wastewater. | ten bacterial strains that utilize cyanide (cn) as a nitrogen source were isolated from cassava factory wastewater after enrichment in a liquid media containing sodium cyanide (1 mm) and glucose (0.2% w/v). the strains could tolerate and grow in cyanide concentrations of up to 5 mm. increased cyanide levels in the media caused an extension of lag phase in the bacterial growth indicating that they need some period of acclimatisation. the rate of cyanide removal by the strains depends on the initi ... | 2015 | 26413045 |
| microbiologic spectrum and susceptibility of isolates in delayed post-cataract surgery endophthalmitis. | the objective of this study was to evaluate the microbiological spectrum and antimicrobial susceptibility of isolates in delayed post-cataract surgery endophthalmitis. a retrospective review of 33 consecutive patients with culture proven delayed post-cataract surgery endophthalmitis was done from january 2006 to march 2013. there were 22 bacterial and eleven fungal cases. common isolates were streptococci (seven cases), coagulase-negative staphylococci (five), gram-negative bacilli (seven), noca ... | 2015 | 26124631 |
| opportunistic pathogens and elements of the resistome that are common in bottled mineral water support the need for continuous surveillance. | several differences concerning bacterial species, opportunistic pathogens, elements of the resistome as well as variations concerning the cfu/ml counts were identified in some of the five most marketed bottled mineral water from araraquara city, são paulo, brazil. two out of five brands tested were confirmed as potential source of opportunistic pathogens, including mycobacterium gordonae, ralstonia picketti and burkholderia cepacia complex (bcc). a total of one hundred and six isolates were reco ... | 2015 | 25803794 |
| understanding the concept of health care-associated pneumonia in lung transplant recipients. | limited data are available regarding the etiologic impact of health care-associated pneumonia (hcap) in lung transplant recipients. therefore, our aim was to evaluate the microbiologic differences between hcap and hospital-acquired pneumonia (hap)/ventilator-associated pneumonia (vap) in lung transplant recipients with a radiographically confirmed diagnosis of pneumonia. | 2015 | 25742187 |
| chemoenzymatic dynamic kinetic resolution: a powerful tool for the preparation of enantiomerically pure alcohols and amines. | chemoenzymatic dynamic kinetic resolution (dkr) constitutes a convenient and efficient method to access enantiomerically pure alcohol and amine derivatives. this perspective highlights the work carried out within this field during the past two decades and pinpoints important avenues for future research. first, the perspective will summarize the more developed area of alcohol dkr, by delineating the way from the earliest proof-of-concept protocols to the current state-of-the-art systems that allo ... | 2015 | 25730714 |
| selenium hyperaccumulators harbor a diverse endophytic bacterial community characterized by high selenium resistance and plant growth promoting properties. | selenium (se)-rich plants may be used to provide dietary se to humans and livestock, and also to clean up se-polluted soils or waters. this study focused on endophytic bacteria of plants that hyperaccumulate selenium (se) to 0.5-1% of dry weight. terminal restriction fragment length polymorphism (t-rflp) analysis was used to compare the diversity of endophytic bacteria of hyperaccumulators stanleya pinnata (brassicaceae) and astragalus bisulcatus (fabaceae) with those from related non-accumulato ... | 2015 | 25784919 |
| impact of alkyl polyglucosides surfactant lutensol gd 70 on modification of bacterial cell surface properties. | alkyl polyglucosides, due to their low toxicity and environmental compatibility, could be used in biodegradation of hydrophobic compounds. in this study, the influence of lutensol gd 70 on the cell hydrophobicity and zeta potential was measured. the particle size distribution and surfactant biodegradation were also investigated. microbacterium sp. strain e19, pseudomonas stutzeri strain 9, and the same strain cultivated in stress conditions were used in studies. adding surfactant to the diesel o ... | 2015 | 25741049 |
| melioidosis diagnostic workshop, 2013. | melioidosis is a severe disease that can be difficult to diagnose because of its diverse clinical manifestations and a lack of adequate diagnostic capabilities for suspected cases. there is broad interest in improving detection and diagnosis of this disease not only in melioidosis-endemic regions but also outside these regions because melioidosis may be underreported and poses a potential bioterrorism challenge for public health authorities. therefore, a workshop of academic, government, and pri ... | 2015 | 25626057 |
| antibiotic resistance profile of non-fermenting gram-negative bacilli isolated from the blood cultures of cancer patients. | | 2015 | 25722625 |
| prevalence and identification of burkholderia pseudomallei and near-neighbor species in the malabar coastal region of india. | accurate identification of pathogens with biowarfare importance requires detection tools that specifically differentiate them from near-neighbor species. burkholderia pseudomallei, the causative agent of a fatal disease melioidosis, is one such biothreat agent whose differentiation from its near-neighbor species is always a challenge. this is because of its phenotypic similarity with other burkholderia species which have a wide spread geographical distribution with shared environmental niches. m ... | 2016 | 27632353 |
| linking microbial community and catabolic gene structures during the adaptation of three contaminated soils under continuous long-term pollutant stress. | three types of contaminated soil from three geographically different areas were subjected to a constant supply of benzene or benzene/toluene/ethylbenzene/xylenes (btex) for a period of 3 months. different from the soil from brazil (bra) and switzerland (sui), the czech republic (cze) soil which was previously subjected to intensive in situ bioremediation displayed only negligible changes in community structure. bra and sui soil samples showed a clear succession of phylotypes. a rapid response to ... | 2016 | 26850298 |
| solvent-free lipase-catalyzed synthesis of diacylgycerols as low-calorie food ingredients. | problems derived from obesity and overweight have recently promoted the development of fat substitutes and other low-calorie foods. on the one hand, fats with short- and medium-chain fatty acids are a source of quick energy, easily hydrolyzable and hardly stored as fat. furthermore, 1,3-diacylglycerols are not hydrolyzed to 2-monoacylglycerols in the gastrointestinal tract, reducing the formation of chylomicron and lowers the serum level of triacylglycerols by decreasing its resynthesis in the e ... | 2016 | 26904539 |
| avian incubation inhibits growth and diversification of bacterial assemblages on eggs. | microbial infection is a critical source of mortality for early life stages of oviparous vertebrates, but parental defenses against infection are less well known. avian incubation has been hypothesized to reduce the risk of trans-shell infection by limiting microbial growth of pathogenic bacteria on eggshells, while enhancing growth of commensal or beneficial bacteria that inhibit or competitively exclude pathogens. we tested this hypothesis by comparing bacterial assemblages on naturally incuba ... | 2009 | 19225566 |
| first report of an extensively drug-resistant vim-2 metallo-β-lactamase-producing brevundimonas diminuta clinical isolate. | in the literature, only three brevundimonas diminuta environmental isolates carrying metallo-β-lactamase genes were recently published. however, so far, no b. diminuta clinical isolates carrying these carbapenem resistance genes have been described. here we report the first vim-2 metallo-β-lactamase-producing b. diminuta clinical isolate obtained from an immunocompromised patient. | 2012 | 22692741 |
| a treatment plant receiving waste water from multiple bulk drug manufacturers is a reservoir for highly multi-drug resistant integron-bearing bacteria. | the arenas and detailed mechanisms for transfer of antibiotic resistance genes between environmental bacteria and pathogens are largely unclear. selection pressures from antibiotics in situations where environmental bacteria and human pathogens meet are expected to increase the risks for such gene transfer events. we hypothesize that waste-water treatment plants (wwtps) serving antibiotic manufacturing industries may provide such spawning grounds, given the high bacterial densities present there ... | 2013 | 24204801 |
| maldi-tof mass spectrometry and identification of new bacteria species in air samples from makkah, saudi arabia. | during the hajj season, respiratory symptoms are very common among pilgrims. here, we investigated the viable bacterial population in air samples collected around the slaughterhouses used during the hajj. | 2014 | 25491533 |
| new pcr-based open reading frame typing method for easy, rapid, and reliable identification of acinetobacter baumannii international epidemic clones without performing multilocus sequence typing. | antimicrobial resistance issues have become a global health concern. the rapid identification of multidrug-resistant microbes, which depends on microbial genomic information, is essential for overcoming growing antimicrobial resistance challenges. however, genotyping methods, such as multilocus sequence typing (mlst), for identifying international epidemic clones of acinetobacter baumannii are not easily performed as routine tests in ordinary clinical laboratories. in this study, we aimed to dev ... | 2014 | 24899031 |
| dynamics of bacterial populations during bench-scale bioremediation of oily seawater and desert soil bioaugmented with coastal microbial mats. | this study describes a bench-scale attempt to bioremediate kuwaiti, oily water and soil samples through bioaugmentation with coastal microbial mats rich in hydrocarbonoclastic bacterioflora. seawater and desert soil samples were artificially polluted with 1% weathered oil, and bioaugmented with microbial mat suspensions. oil removal and microbial community dynamics were monitored. in batch cultures, oil removal was more effective in soil than in seawater. hydrocarbonoclastic bacteria associated ... | 2016 | 26751253 |
| detection and diversity of expressed denitrification genes in estuarine sediments after reverse transcription-pcr amplification from mrna. | the expression of five denitrification genes coding for two nitrate reductases (narg and napa), two nitrite reductases (nirs and nirk), and nitrous oxide reductase (nosz) was analyzed by reverse transcription (rt)-pcr of mrna extracted from two sediment samples obtained in the river colne estuary (united kingdom), which receives high nitrogen inputs and for which high denitrification rates have been observed. the presence of all five genes in both sediment samples was confirmed by pcr amplificat ... | 2002 | 12324352 |
| spatially oscillating activity and microbial succession of mercury-reducing biofilms in a technical-scale bioremediation system. | mercury-contaminated chemical wastewater of a mercury cell chloralkali plant was cleaned on site by a technical-scale bioremediation system. microbial mercury reduction of soluble hg(ii) to precipitating hg(0) decreased the mercury load of the wastewater during its flow through the bioremediation system by up to 99%. the system consisted of a packed-bed bioreactor, where most of the wastewater's mercury load was retained, and an activated carbon filter, where residual mercury was removed from th ... | 2002 | 11916716 |
| use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. | | 2005 | 16151072 |
| dynamics of an oligotrophic bacterial aquifer community during contact with a groundwater plume contaminated with benzene, toluene, ethylbenzene, and xylenes: an in situ mesocosm study. | an in situ mesocosm system was designed to monitor the in situ dynamics of the microbial community in polluted aquifers. the mesocosm system consists of a permeable membrane pocket filled with aquifer material and placed within a polypropylene holder, which is inserted below groundwater level in a monitoring well. after a specific time period, the microcosm is recovered from the well and its bacterial community is analyzed. using this system, we examined the effect of benzene, toluene, ethylbenz ... | 2005 | 16000793 |
| nitrilase enzymes and their role in plant-microbe interactions. | nitrilase enzymes (nitrilases) catalyse the hydrolysis of nitrile compounds to the corresponding carboxylic acid and ammonia, and have a wide range of industrial and biotechnological applications, including the synthesis of industrially important carboxylic acids and bioremediation of cyanide and toxic nitriles. nitrilases are produced by a wide range of organisms, including plants, bacteria and fungi, but despite their biotechnological importance, the role of these enzymes in living organisms i ... | 2009 | 21255276 |
| the pha depolymerase engineering database: a systematic analysis tool for the diverse family of polyhydroxyalkanoate (pha) depolymerases. | polyhydroxyalkanoates (phas) can be degraded by many microorganisms using intra- or extracellular pha depolymerases. pha depolymerases are very diverse in sequence and substrate specificity, but share a common alpha/beta-hydrolase fold and a catalytic triad, which is also found in other alpha/beta-hydrolases. | 2009 | 19296857 |
| abundances and potential activities of nitrogen cycling microbial communities along a chronosequence of a glacier forefield. | glacier forefields are ideal ecosystems to study the development of nutrient cycles as well as single turnover processes during soil development. in this study, we examined the ecology of the microbial nitrogen (n) cycle in bulk soil samples from a chronosequence of the damma glacier, switzerland. major processes of the n cycle were reconstructed on the genetic as well as the potential enzyme activity level at sites of the chronosequence that have been ice-free for 10, 50, 70, 120 and 2000 years ... | 2010 | 21124490 |
| abundances and potential activities of nitrogen cycling microbial communities along a chronosequence of a glacier forefield. | glacier forefields are ideal ecosystems to study the development of nutrient cycles as well as single turnover processes during soil development. in this study, we examined the ecology of the microbial nitrogen (n) cycle in bulk soil samples from a chronosequence of the damma glacier, switzerland. major processes of the n cycle were reconstructed on the genetic as well as the potential enzyme activity level at sites of the chronosequence that have been ice-free for 10, 50, 70, 120 and 2000 years ... | 2010 | 21124490 |
| use of pseudomonas stutzeri and candida utilis in the improvement of the conditions of artemia culture and protection against pathogens. | to evaluate the effect of two bacterial strains isolated from artemia cysts and yeast (candida utilis) on the survival, growth and total biomass production of its larvae, challenge tests were performed with candida utilis, pseudomonas stutzeri and pasteurella haemolityca. in addition, a pathogenic strain of vibrio alginolyticus was tested for comparative purposes. pseudomonas stutzeri and candida utilis have no impact on survival, but enhance growth and total biomass production of the larvae. ho ... | 2010 | 24031470 |
| occurrence of plasmids in the aromatic degrading bacterioplankton of the baltic sea. | plasmids are mobile genetic elements that provide their hosts with many beneficial traits including in some cases the ability to degrade different aromatic compounds. to fulfill the knowledge gap regarding catabolic plasmids of the baltic sea water, a total of 209 biodegrading bacterial strains were isolated and screened for the presence of these mobile genetic elements. we found that both large and small plasmids are common in the cultivable baltic sea bacterioplankton and are particularly prev ... | 2011 | 24710296 |
| comparative evaluation of bruker biotyper and bd phoenix systems for identification of bacterial pathogens associated with urinary tract infections. | the bruker biotyper and bd phoenix systems were evaluated for identification of 1,024 bacterial urinary tract isolates. the biotyper and phoenix systems correctly identified 99.9% and 99.5% to the genus level and 99.1% and 98.5% to the species level, respectively. both systems provide reliable results, and the biotyper system offers a rapid tool for urine bacterial isolate identification. | 2011 | 21918029 |
| decontamination of mda reagents for single cell whole genome amplification. | single cell genomics is a powerful and increasingly popular tool for studying the genetic make-up of uncultured microbes. a key challenge for successful single cell sequencing and analysis is the removal of exogenous dna from whole genome amplification reagents. we found that uv irradiation of the multiple displacement amplification (mda) reagents, including the phi29 polymerase and random hexamer primers, effectively eliminates the amplification of contaminating dna. the methodology is quick, s ... | 2011 | 22028825 |
| changes in diversity and functional gene abundances of microbial communities involved in nitrogen fixation, nitrification, and denitrification in a tidal wetland versus paddy soils cultivated for different time periods. | in many areas of china, tidal wetlands have been converted into agricultural land for rice cultivation. however, the consequences of land use changes for soil microbial communities are poorly understood. therefore, we investigated bacterial and archaeal communities involved in inorganic nitrogen turnover (nitrogen fixation, nitrification, and denitrification) based on abundances and relative species richness of the corresponding functional genes along a soil chronosequence ranging between 50 and ... | 2011 | 21764972 |
| rapid detection of carbapenemase-producing pseudomonas spp. | a novel biochemical technique, the carba np test, has been evaluated to detect carbapenemase production in pseudomonas spp. this test was specific (100%), sensitive (94.4%), and rapid (<2 h). this cost-effective test, which could be implemented in any microbiology laboratory, offers a reliable technique for identification of carbapenemase-producing pseudomonas spp. | 2012 | 22972829 |
| denitrification and environmental factors influencing nitrate removal in guaymas basin hydrothermally altered sediments. | we measured potential nitrate removal and denitrification rates in hydrothermally altered sediments inhabited by beggiatoa mats and adjacent brown oil stained sediments from the guaymas basin, gulf of california. sediments with beggiatoa maintained slightly higher rates of potential denitrification than did brown sediments at 31.2 ± 12.1 versus 21.9 ± 1.4 µm n day(-1), respectively. in contrast, the nitrate removal rates in brown sediments were higher than those observed in mat-hosting sediments ... | 2012 | 23112796 |
| a culture-independent approach to unravel uncultured bacteria and functional genes in a complex microbial community. | most microorganisms in nature are uncultured with unknown functionality. sequence-based metagenomics alone answers 'who/what are there?' but not 'what are they doing and who is doing it and how?'. function-based metagenomics reveals gene function but is usually limited by the specificity and sensitivity of screening strategies, especially the identification of clones whose functional gene expression has no distinguishable activity or phenotypes. a 'biosensor-based genetic transducer' (bgt) techn ... | 2012 | 23082176 |
| potent antibacterial antisense peptide-peptide nucleic acid conjugates against pseudomonas aeruginosa. | pseudomonas aeruginosa is an opportunistic pathogen causing severe infections in hospital settings, especially with immune compromised patients, and the increasing prevalence of multidrug resistant strains urges search for new drugs with novel mechanisms of action. in this study we introduce antisense peptide-peptide nucleic acid (pna) conjugates as antibacterial agents against p. aeruginosa. we have designed and optimized antisense peptide-pna conjugates targeting the translation initiation reg ... | 2012 | 23030590 |
| genome sequence of a highly efficient aerobic denitrifying bacterium, pseudomonas stutzeri t13. | pseudomonas stutzeri t13 is a highly efficient aerobic denitrifying bacterium. information about the genome of this aerobic denitrifying bacterium has been limited until now. we present the draft genome of p. stutzeri t13. the results could provide further insight into the aerobic denitrification mechanism in strain t13. | 2012 | 23012292 |
| fatty acid biosynthesis in pseudomonas aeruginosa is initiated by the faby class of β-ketoacyl acyl carrier protein synthases. | the prototypical type ii fatty acid synthesis (fas) pathway in bacteria utilizes two distinct classes of β-ketoacyl synthase (kas) domains to assemble long-chain fatty acids, the kasiii domain for initiation and the kasi/ii domain for elongation. the central role of fas in bacterial viability and virulence has stimulated significant effort toward developing kas inhibitors, particularly against the kasiii domain of the β-acetoacetyl-acyl carrier protein (acp) synthase fabh. herein, we show that t ... | 2012 | 22753059 |
| genome sequence of the broad-host-range pseudomonas phage φ-s1. | the broad-host-range lytic pseudomonas phage φ-s1 possess a 40,192 bp double-stranded dna (dsdna) genome of 47 open reading frames (orfs) and belongs to the family podoviridae, subfamily autographivirinae, genus t7likevirus. | 2012 | 22923799 |
| multiple transcription-activating sequences regulate the rsmz regulatory small rna of pseudomonas brassicacearum. | the muts-rpos region is known to be a highly polymorphic segment of the chromosome owing to horizontal gene transfer and evolutionary processes. in pseudomonas, muts-fdxa-rsmz-rpos organization is highly conserved, as well as the promoter region of the rsmz small rna (srna)-encoding gene. one exception to this conservation is in pseudomonas brassicacearum, where a 308-nucleotide (nt) sequence, predicted to form a hairpin structure in single-stranded dna (ssdna), is inserted between the rpos and ... | 2012 | 22773788 |
| membrane vesicle formation as a multiple-stress response mechanism enhances pseudomonas putida dot-t1e cell surface hydrophobicity and biofilm formation. | among the adaptive responses of bacteria to rapid changes in environmental conditions, those of the cell envelope are known to be the most crucial. therefore, several mechanisms with which bacteria change their cell surface and membranes in the presence of different environmental stresses have been elucidated. among these mechanisms, the release of outer membrane vesicles (mv) in gram-negative bacteria has attracted particular research interest because of its involvement in pathogenic processes, ... | 2012 | 22752175 |
| pseudomonas aeruginosa possesses two putative type i signal peptidases, lepb and pa1303, each with distinct roles in physiology and virulence. | type i signal peptidases (spases) cleave signal peptides from proteins during translocation across biological membranes and hence play a vital role in cellular physiology. spase activity is also of fundamental importance to the pathogenesis of infection for many bacteria, including pseudomonas aeruginosa, which utilizes a variety of secreted virulence factors, such as proteases and toxins. p. aeruginosa possesses two noncontiguous spase homologues, lepb (pa0768) and pa1303, which share 43% amino ... | 2012 | 22730125 |
| a novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in escherichia coli and tobacco plants. | a key enzyme in the shikimate pathway, 5-enolpyruvylshikimate-3-phosphate synthase (epsps) is the primary target of the broad-spectrum herbicide glyphosate. identification of new aroa genes coding for epsps with a high level of glyphosate tolerance is essential for the development of glyphosate-tolerant crops. in the present study, the glyphosate tolerance of five bacterial aroa genes was evaluated in the e. coli aroa-defective strain er2799 and in transgenic tobacco plants. all five aroa genes ... | 2012 | 22715408 |
| crystal structures of phosphite dehydrogenase provide insights into nicotinamide cofactor regeneration. | the enzyme phosphite dehydrogenase (ptdh) catalyzes the nad(+)-dependent conversion of phosphite to phosphate and represents the first biological catalyst that has been shown to conduct the enzymatic oxidation of phosphorus. despite investigation for more than a decade into both the mechanism of its unusual reaction and its utility in cofactor regeneration, there has been a lack of any structural data for ptdh. here we present the cocrystal structure of an engineered thermostable variant of ptdh ... | 2012 | 22564171 |
| subtilase sprp exerts pleiotropic effects in pseudomonas aeruginosa. | the open reading frame pa1242 in the genome of pseudomonas aeruginosa pao1 encodes a putative protease belonging to the peptidase s8 family of subtilases. the respective enzyme termed sprp consists of an n-terminal signal peptide and a so-called s8 domain linked by a domain of unknown function (duf). presumably, this duf domain defines a discrete class of pseudomonas proteins as homologous domains can be identified almost exclusively in proteins of the genus pseudomonas. the sprp gene was expres ... | 2013 | 24376018 |
| subtilase sprp exerts pleiotropic effects in pseudomonas aeruginosa. | the open reading frame pa1242 in the genome of pseudomonas aeruginosa pao1 encodes a putative protease belonging to the peptidase s8 family of subtilases. the respective enzyme termed sprp consists of an n-terminal signal peptide and a so-called s8 domain linked by a domain of unknown function (duf). presumably, this duf domain defines a discrete class of pseudomonas proteins as homologous domains can be identified almost exclusively in proteins of the genus pseudomonas. the sprp gene was expres ... | 2013 | 24376018 |
| evolution of probiotics in aquatic world: potential effects, the current status in egypt and recent prospectives. | the increase in the human population in addition to the massive demand for protein of animal origin forced the authorities to seek for additional sources of feed supplies. aquaculture is the world worth coming expansion to compensate the shortage in animal protein. feed in aquaculture plays an important role in the production cycle and exert threshold on both practical and economic aspects. feed additive sectors are expanding day after day to achieve better growth and health for fish and shrimp ... | 2013 | 26644914 |
| evolution of probiotics in aquatic world: potential effects, the current status in egypt and recent prospectives. | the increase in the human population in addition to the massive demand for protein of animal origin forced the authorities to seek for additional sources of feed supplies. aquaculture is the world worth coming expansion to compensate the shortage in animal protein. feed in aquaculture plays an important role in the production cycle and exert threshold on both practical and economic aspects. feed additive sectors are expanding day after day to achieve better growth and health for fish and shrimp ... | 2013 | 26644914 |
| evaluation of a fret-peptide substrate to predict virulence in pseudomonas aeruginosa. | pseudomonas aeruginosa produces a number of proteases that are associated with virulence and disease progression. a substrate able to detect p. aeruginosa-specific proteolytic activity could help to rapidly alert clinicians to the virulence potential of individual p. aeruginosa strains. for this purpose we designed a set of p. aeruginosa-specific fluorogenic substrates, comprising fluorescence resonance energy transfer (fret)-labeled peptides, and evaluated their applicability to p. aeruginosa v ... | 2013 | 24303047 |
| the genome of pseudomonas fluorescens strain r124 demonstrates phenotypic adaptation to the mineral environment. | microbial adaptation to environmental conditions is a complex process, including acquisition of positive traits through horizontal gene transfer or the modification of existing genes through duplication and/or mutation. in this study, we examined the adaptation of a pseudomonas fluorescens isolate (r124) from the nutrient-limited mineral environment of a silica cave in comparison with p. fluorescens isolates from surface soil and the rhizosphere. examination of metal homeostasis gene pathways de ... | 2013 | 23995634 |
| phylogenomic analysis identifies gene gains that define salmonella enterica subspecies i. | comparative methods for analyzing whole genome sequence (wgs) data enable us to assess the genetic information available for reconstructing the evolutionary history of pathogens. we used the comparative approach to determine diagnostic genes for salmonella enterica subspecies i. s. enterica subsp. i strains are known to infect warm-blooded organisms regularly while its close relatives tend to infect only cold-blooded organisms. we found 71 genes gained by the common ancestor of salmonella enteri ... | 2013 | 24204679 |
| influence of root exudates on the bacterial degradation of chlorobenzoic acids. | degradation of chlorobenzoic acids (e.g., products of microbial degradation of pcb) by strains of microorganisms isolated from pcb contaminated soils was assessed. from seven bulk-soil isolates two strains unique in ability to degrade a wider range of chlorobenzoic acids than others were selected, individually and even in a complex mixture of 11 different chlorobenzoic acids. such a feature is lacking in most tested degraders. to investigate the influence of vegetation on chlorobenzoic acids deg ... | 2013 | 24222753 |
| host-microbe interactions in caenorhabditis elegans. | a good understanding of how microbes interact with hosts has a direct bearing on our capability of fighting infectious microbial pathogens and making good use of beneficial ones. among the model organisms used to study reciprocal actions among microbes and hosts, c. elegans may be the most advantageous in the context of its unique attributes such as the short life cycle, easiness of laboratory maintenance, and the availability of different genetic mutants. this review summarizes the recent advan ... | 2013 | 23984180 |
| complete sequence of poz176, a 500-kilobase incp-2 plasmid encoding imp-9-mediated carbapenem resistance, from outbreak isolate pseudomonas aeruginosa 96. | pseudomonas aeruginosa 96 (pa96) was isolated during a multicenter surveillance study in guangzhou, china, in 2000. whole-genome sequencing of this outbreak strain facilitated analysis of its incp-2 carbapenem-resistant plasmid, poz176. the plasmid had a length of 500,839 bp and an average percent g+c content of 57%. of the 618 predicted open reading frames, 65% encode hypothetical proteins. the poz176 backbone is not closely related to any plasmids thus far sequenced, but some similarity to pqb ... | 2013 | 23716048 |
| comparative genomic analysis of four representative plant growth-promoting rhizobacteria in pseudomonas. | some pseudomonas strains function as predominant plant growth-promoting rhizobacteria (pgpr). within this group, pseudomonas chlororaphis and pseudomonas fluorescens are non-pathogenic biocontrol agents, and some pseudomonas aeruginosa and pseudomonas stutzeri strains are pgpr. p. chlororaphis gp72 is a plant growth-promoting rhizobacterium with a fully sequenced genome. we conducted a genomic analysis comparing gp72 with three other pseudomonad pgpr: p. fluorescens pf-5, p. aeruginosa m18, and ... | 2013 | 23607266 |
| new constitutive vectors: useful genetic engineering tools for biocatalysis. | constitutive vectors are useful tools for genetic engineering. two constitutive vectors with high levels of expression and broad host ranges were developed and used in a range of pseudomonas hosts. the vectors showed superior characteristics compared to the inducible vectors as well as the potential to be used as improved genetic tools for biocatalysis. | 2013 | 23416993 |
| crcz and crcx regulate carbon source utilization in pseudomonas syringae pathovar tomato strain dc3000. | small non-coding rnas (ncrnas) are important components of many regulatory pathways in bacteria and play key roles in regulating factors important for virulence. carbon catabolite repression control is modulated by small rnas (crcz or crcz and crcy) in pseudomonas aeruginosa and pseudomonas putida. in this study, we demonstrate that expression of crcz and crcx (formerly designated psr1 and psr2, respectively) is dependent upon rpon together with the two-component system cbrab, and is influenced ... | 2013 | 23353577 |
| recruitment and rearrangement of three different genetic determinants into a conjugative plasmid increase copper resistance in pseudomonas syringae. | we describe the genetic organization of a copper-resistant plasmid containing copg and cuscba genes in the plant pathogen pseudomonas syringae. chromosomal variants of czccba and a plasmid variant of cuscba were present in different p. syringae pathovar strains. transformation of the copper-sensitive pseudomonas syringae pv. syringae ff5 strain with copg or cuscba conferred copper resistance, and quantitative real-time pcr (qrt-pcr) experiments confirmed their induction by copper. | 2013 | 23183969 |
| association with soil bacteria enhances p38-dependent infection resistance in caenorhabditis elegans. | the importance of our inner microbial communities for proper immune responses against invading pathogens is now well accepted, but the mechanisms underlying this protection are largely unknown. in this study, we used caenorhabditis elegans to investigate such mechanisms. since very little is known about the microbes interacting with c. elegans in its natural environment, we began by taking the first steps to characterize the c. elegans microbiota. we established a natural-like environment in whi ... | 2013 | 23230286 |
| the biology of habitat dominance; can microbes behave as weeds? | competition between microbial species is a product of, yet can lead to a reduction in, the microbial diversity of specific habitats. microbial habitats can resemble ecological battlefields where microbial cells struggle to dominate and/or annihilate each other and we explore the hypothesis that (like plant weeds) some microbes are genetically hard-wired to behave in a vigorous and ecologically aggressive manner. these 'microbial weeds' are able to dominate the communities that develop in fertile ... | 2013 | 23336673 |
| influence of organic solvents on catalytic behaviors and cell morphology of whole-cell biocatalysts for synthesis of 5'-arabinocytosine laurate. | a whole-cell based method was developed for the regioselective synthesis of arabinocytosine laurate. among the seven kinds of bacteria strains tested in the acylation reaction, pseudomonas fluorescens gave the highest productivity and a higher 5'-regioselectivity than 99%. compared with pure organic solvents, the use of organic solvent mixtures greatly promoted the yield of the whole-cell catalyzed reaction, but showed little influence on the 5'-regioselectivity. of all the tested solvent mixtur ... | 2014 | 25136983 |
| emergence of vim-2 and imp-15 carbapenemases and inactivation of oprd gene in carbapenem-resistant pseudomonas aeruginosa clinical isolates from lebanon. | we report here the emergence of vim-2 and imp-15 carbapenemases in a series of clinical isolates of carbapenem-resistant pseudomonas aeruginosa in lebanon. we also describe the disruption of the oprd gene by either mutations or insertion sequence (is) elements ispa1328 and ispre2 isoform. our study reemphasizes a rapid dissemination of the vim-2 carbapenemase-encoding gene in clinical isolates of p. aeruginosa in the mediterranean basin. | 2014 | 24913164 |
| prophylaxis with levofloxacin: impact on bacterial susceptibility and epidemiology in a hematopoietic stem cell transplant unit. | the emergence of resistance has been demonstrated in cancer treatment centers where prophylaxis with fluoroquinolone is used. | 2014 | 24624034 |
| pseudomonas aeruginosa genome evolution in patients and under the hospital environment. | pseudomonas aeruginosa is a gram-negative environmental species and an opportunistic microorganism, establishing itself in vulnerable patients, such as those with cystic fibrosis (cf) or those hospitalized in intensive care units (icu). it has become a major cause of nosocomial infections worldwide and a serious threat to public health because of overuse and misuse of antibiotics that have selected highly resistant strains against which very few therapeutic options exist. herein is illustrated t ... | 2014 | 25437802 |
| flavoprotein monooxygenases for oxidative biocatalysis: recombinant expression in microbial hosts and applications. | external flavoprotein monooxygenases comprise a group of flavin-dependent oxidoreductases that catalyze the insertion of one atom of molecular oxygen into an organic substrate and the second atom is reduced to water. these enzymes are involved in a great number of metabolic pathways both in prokaryotes and eukaryotes. flavoprotein monooxygenases have attracted the attention of researchers for several decades and the advent of recombinant dna technology caused a great progress in the field. these ... | 2014 | 24567729 |
| pseudomonas putida csv86: a candidate genome for genetic bioaugmentation. | pseudomonas putida csv86, a plasmid-free strain possessing capability to transfer the naphthalene degradation property, has been explored for its metabolic diversity through genome sequencing. the analysis of draft genome sequence of csv86 (6.4 mb) revealed the presence of genes involved in the degradation of naphthalene, salicylate, benzoate, benzylalcohol, p-hydroxybenzoate, phenylacetate and p-hydroxyphenylacetate on the chromosome thus ensuring the stability of the catabolic potential. moreo ... | 2014 | 24475028 |
| a moderate toxin, grat, modulates growth rate and stress tolerance of pseudomonas putida. | chromosomal toxin-antitoxin (ta) systems are widespread among free-living bacteria and are supposedly involved in stress tolerance. here, we report the first ta system identified in the soil bacterium pseudomonas putida. the system, encoded by the loci pp1586-pp1585, is conserved in pseudomonads and belongs to the higba family. the new ta pair was named grata for the growth rate-affecting ability of grat and the antidote activity of graa. the grata system shares many features common to previousl ... | 2014 | 24163334 |
| retrospective molecular analysis of dim-1 metallo-β-lactamase discovered in pseudomonas stutzeri from india in 2000. | among 220 clinical isolates of gram-negative bacilli collected in india during 2000, 22 strains showing elevated imipenem mics were evaluated for carbapenemase production. one dim-1-producing pseudomonas stutzeri isolate was detected, and no other carbapenemase-encoding genes were identified. this detection of a dim-1-producing p. stutzeri isolate from india predating the finding of this gene in the index dutch strain and the very recent detection of dim-1 in africa suggest an unidentified envir ... | 2014 | 24145536 |
| pseudomonas sp. lz-q continuously degrades phenanthrene under hypersaline and hyperalkaline condition in a membrane bioreactor system. | | 2015 | 27340693 |
| pseudomonas sp. lz-q continuously degrades phenanthrene under hypersaline and hyperalkaline condition in a membrane bioreactor system. | | 2015 | 27340693 |
| isolation and genetic analysis of multidrug resistant bacteria from diabetic foot ulcers. | severe diabetic foot ulcers (dfus) patients visiting sir sunderlal hospital, banaras hindu university, varanasi, were selected for this study. bacteria were isolated from swab and deep tissue of 42 patients, for examining their prevalence and antibiotic sensitivity. dfus of majority of the patients were found infected with enterococcus spp. (47.61%), escherichia coli (35.71%), staphylococcus spp. (33.33%), alcaligenes spp. (30.95%), pseudomonas spp. (30.95%), and stenotrophomonas spp. (30.95%). ... | 2015 | 26779134 |
| isolation and genetic analysis of multidrug resistant bacteria from diabetic foot ulcers. | severe diabetic foot ulcers (dfus) patients visiting sir sunderlal hospital, banaras hindu university, varanasi, were selected for this study. bacteria were isolated from swab and deep tissue of 42 patients, for examining their prevalence and antibiotic sensitivity. dfus of majority of the patients were found infected with enterococcus spp. (47.61%), escherichia coli (35.71%), staphylococcus spp. (33.33%), alcaligenes spp. (30.95%), pseudomonas spp. (30.95%), and stenotrophomonas spp. (30.95%). ... | 2015 | 26779134 |
| degradation of benzene by pseudomonas veronii 1ydbtex2 and 1yb2 is catalyzed by enzymes encoded in distinct catabolism gene clusters. | pseudomonas veronii 1ydbtex2, a benzene and toluene degrader, and pseudomonas veronii 1yb2, a benzene degrader, have previously been shown to be key players in a benzene-contaminated site. these strains harbor unique catabolic pathways for the degradation of benzene comprising a gene cluster encoding an isopropylbenzene dioxygenase where genes encoding downstream enzymes were interrupted by stop codons. extradiol dioxygenases were recruited from gene clusters comprising genes encoding a 2-hydrox ... | 2015 | 26475106 |
| degradation of benzene by pseudomonas veronii 1ydbtex2 and 1yb2 is catalyzed by enzymes encoded in distinct catabolism gene clusters. | pseudomonas veronii 1ydbtex2, a benzene and toluene degrader, and pseudomonas veronii 1yb2, a benzene degrader, have previously been shown to be key players in a benzene-contaminated site. these strains harbor unique catabolic pathways for the degradation of benzene comprising a gene cluster encoding an isopropylbenzene dioxygenase where genes encoding downstream enzymes were interrupted by stop codons. extradiol dioxygenases were recruited from gene clusters comprising genes encoding a 2-hydrox ... | 2015 | 26475106 |
| diversity of pseudomonas genomes, including populus-associated isolates, as revealed by comparative genome analysis. | the pseudomonas genus contains a metabolically versatile group of organisms that are known to occupy numerous ecological niches, including the rhizosphere and endosphere of many plants. their diversity influences the phylogenetic diversity and heterogeneity of these communities. on the basis of average amino acid identity, comparative genome analysis of >1,000 pseudomonas genomes, including 21 pseudomonas strains isolated from the roots of native populus deltoides (eastern cottonwood) trees resu ... | 2015 | 26519390 |
| diversity of pseudomonas genomes, including populus-associated isolates, as revealed by comparative genome analysis. | the pseudomonas genus contains a metabolically versatile group of organisms that are known to occupy numerous ecological niches, including the rhizosphere and endosphere of many plants. their diversity influences the phylogenetic diversity and heterogeneity of these communities. on the basis of average amino acid identity, comparative genome analysis of >1,000 pseudomonas genomes, including 21 pseudomonas strains isolated from the roots of native populus deltoides (eastern cottonwood) trees resu ... | 2015 | 26519390 |
| fleq dna binding consensus sequence revealed by studies of fleq-dependent regulation of biofilm gene expression in pseudomonas aeruginosa. | the transcription factor fleq from pseudomonas aeruginosa derepresses expression of genes involved in biofilm formation when intracellular levels of the second messenger cyclic diguanosine monophosphate (c-di-gmp) are high. fleq also activates transcription of flagellar genes, and the expression of these genes is highest at low intracellular c-di-gmp. fleq thus plays a central role in mediating the transition between planktonic and biofilm lifestyles of p. aeruginosa. previous work showed that f ... | 2015 | 26483521 |
| fleq dna binding consensus sequence revealed by studies of fleq-dependent regulation of biofilm gene expression in pseudomonas aeruginosa. | the transcription factor fleq from pseudomonas aeruginosa derepresses expression of genes involved in biofilm formation when intracellular levels of the second messenger cyclic diguanosine monophosphate (c-di-gmp) are high. fleq also activates transcription of flagellar genes, and the expression of these genes is highest at low intracellular c-di-gmp. fleq thus plays a central role in mediating the transition between planktonic and biofilm lifestyles of p. aeruginosa. previous work showed that f ... | 2015 | 26483521 |
| persistence of nasal colonization with human pathogenic bacteria and associated antimicrobial resistance in the german general population. | the nares represent an important bacterial reservoir for endogenous infections. this study aimed to assess the prevalence of nasal colonization by different important pathogens, the associated antimicrobial susceptibility and risk factors. we performed a prospective cohort study among 1878 nonhospitalized volunteers recruited from the general population in germany. participants provided nasal swabs at three time points (each separated by 4-6 months). staphylococcus aureus, enterobacteriaceae and ... | 2015 | 26862431 |