| increased risk of transmission of hepatitis c in open heart surgery compared with vascular and pulmonary surgery. | we report a case of patient-to-surgeon transmission of hepatitis c virus (hcv), and the subsequent transmission of hcv to surgical patients. | 2010 | 20971233 |
| ultrasound echo is related to stress and strain in tendon. | the mechanical behavior of tendons has been well studied in vitro. a noninvasive method to acquire mechanical data would be highly beneficial. elastography has been a promising method of gathering in vivo tissue mechanical behavior, but it has inherent limitations. this study presents acoustoelasticity as an alternative ultrasound-based method of measuring tendon stress and strain by reporting a relationship between ultrasonic echo intensity (b-mode ultrasound image brightness) and mechanical be ... | 2010 | 21030024 |
| specificity and kinetics of norovirus binding to magnetic bead-conjugated histo-blood group antigens. | to characterize the specificity and effect of ph and ionic strength on the kinetics of virus binding to histo-blood group antigens (hbga)-conjugated magnetic beads. | 2010 | 21040268 |
| a crucial role for b and t lymphocyte attenuator in preventing the development of cd4+ t cell-mediated herpetic stromal keratitis. | to investigate the effect of the b and t lymphocyte attenuator (btla; cd272) on cluster of differentiation (cd)4(+) t cell-mediated corneal immunopathology during murine herpetic stromal keratitis (hsk). | 2010 | 21042564 |
| aspartate 141 is the fourth ligand of the oxygen-sensing [4fe-4s]2+ cluster of bacillus subtilis transcriptional regulator fnr. | the bacillus subtilis redox regulator fnr controls genes of the anaerobic metabolism in response to low oxygen tension. an unusual structure for the oxygen-sensing [4fe-4s](2+) cluster was detected by a combination of genetic experiments with uv-visible and mössbauer spectroscopy. asp-141 was identified as the fourth iron-sulfur cluster ligand besides three cys residues. exchange of asp-141 with ala abolished functional in vivo complementation of an fnr knock-out strain by the mutagenized fnr ge ... | 2010 | 21068385 |
| oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of chlorella kessleri. | we studied the toxicity of a glyphosate formulation and provide evidence of metabolic alterations due to oxidative stress caused in a chlorella kessleri tolerant strain by exposure to the herbicide. after 96h of exposure to increasing concentrations of the herbicide (0-70mgl(-1)) with alkylaryl polyglycol ether surfactant, growth was inhibited (ec50-96h 55.62mgl(-1)). glyphosate increased protein and malondialdehyde content which was significantly higher than in the control at 50-70mgl(-1). supe ... | 2010 | 21074853 |
| a statistical approach for the enhanced production of alkaline protease showing fibrinolytic activity from a newly isolated gram-negative bacillus sp. strain as-s20-i. | an alkaline-fibrinolytic protease-producing bacterial strain (as-s20-i) isolated from a soil sample in assam was a gram-negative rod and grown at temperatures ranging from 25 to 55 °c, and ph 6.5 to 11.0. taxonomic identification of isolated strain by polyphasic approach (phenotypic characterization, chemotaxonomic properties, and ribotyping data of the strain) suggested that it belongs to the genus bacillus, for which the name bacillus sp. strain as-s20-i (mtcc 8961) was proposed. the initial s ... | 2010 | 21078421 |
| derepression of a baker's yeast strain for maltose utilization is associated with severe deregulation of hxt gene expression. | we undertook to improve an industrial saccharomyces cerevisiae strain by derepressing it for maltose utilization in the presence of high glucose concentrations. | 2010 | 21091593 |
| loss of heterozygosity at an unlinked genomic locus is responsible for the phenotype of a candida albicans sap4δ sap5δ sap6δ mutant. | the diploid genome of the pathogenic yeast candida albicans exhibits a high degree of heterozygosity. genomic alterations that result in a loss of heterozygosity at specific loci may affect phenotypes and confer a selective advantage under certain conditions. such genomic rearrangements can also occur during the construction of c. albicans mutants and remain undetected. the sap2 gene on chromosome r encodes a secreted aspartic protease that is induced and required for growth of c. albicans when ... | 2010 | 21097666 |
| [virus detection and viral location in brain tissue of sulking mice infected recombinant rabies virus by frozen section]. | to observe the existence and location of the recombinant rabies virus in the hippocampus of the sulking mice infected recombinant rabies virus. | 2010 | 21110420 |
| [preparation of vero cell-adapted influenza h5n1 virus strain by genetic reassortment]. | to prepare vero cell-adapted influenza h5n1 virus strain by genetic reassortment and produce influenza h5n1 vaccine using vero cell as a substrate. | 2010 | 21110431 |
| molecular epidemiology of vancomycin-resistant enterococcus faecium bloodstream infections among patients with neutropenia over a 6-year period in south korea. | over a 6-year period (march 2000-february 2006), there were 60 vancomycin-resistant enterococcus faecium (vref) bloodstream infections (bsis) in a hematology unit, accounting for 83.3% of all vref bsis in the hospital. we investigated 49 vref isolates causing bsis in patients with neutropenia to understand the molecular epidemiology at this unit. all isolates had the vana genotype. pulsed-field gel electrophoresis typing revealed high clonal diversity (23 types with nine clusters comprising 35 i ... | 2010 | 21128837 |
| cofactor trapping, a new method to produce flavin mononucleotide. | we have purified flavin mononucleotide (fmn) from a flavoprotein-overexpressing escherichia coli strain by cofactor trapping. this approach uses an overexpressed flavoprotein to trap fmn, which is thus removed from the cascade regulating fmn production in e. coli. this, in turn, allows the isolation of highly pure fmn. | 2010 | 21131527 |
| [knockout and function analysis of pqql gene in escherichia coli]. | to confirm the involvement of pqql gene of escherichia coli in pqq biosynthesis, a pqql deletion mutant of e. coli dh5alpha was constructed and investigated. | 2010 | 21141474 |
| nasopharyngeal colonization by neisseria lactamica and induction of protective immunity against neisseria meningitidis. | natural immunity to neisseria meningitidis may result from nasopharyngeal carriage of closely related commensals, such as neisseria lactamica. | 2011 | 21148522 |
| identification, activity and disulfide connectivity of c-di-gmp regulating proteins in mycobacterium tuberculosis. | c-di-gmp, a bacterial second messenger plays a key role in survival and adaptation of bacteria under different environmental conditions. the level of c-di-gmp is regulated by two opposing activities, namely diguanylate cyclase (dgc) and phosphodiesterase (pde-a) exhibited by ggdef and eal domain, respectively in the same protein. previously, we reported a bifunctional ggdef-eal domain protein, msdgc-1 from mycobacterium smegmatis showing both these activities (kumar and chatterji, 2008). in this ... | 2010 | 21151497 |
| characterization by restriction fragment length polymorphism and sequence analysis of field and vaccine strains of infectious laryngotracheitis virus involved in severe outbreaks. | at the end of 2002 and throughout 2003, there was a severe outbreak of infectious laryngotracheitis (ilt) in an intensive production area of commercial hens in the sao paulo state of brazil. ilt virus was isolated from 28 flocks, and 21 isolates were genotyped by polymerase chain reaction and restriction fragment length polymorphism (pcr-rflp) using four genes and eight restriction enzymes, and by partial sequencing of the infected cell protein 4 (icp4) and thymidine kinase (tk) genes. three gro ... | 2010 | 21154050 |
| varicella zoster disease of the central nervous system: epidemiological, clinical, and laboratory features 10 years after the introduction of the varicella vaccine. | since the introduction of live attenuated varicella zoster virus (vzv) vaccine in 1995 there has been a significant reduction in varicella incidence and its associated complications, but the impact on vzv-associated central nervous system (cns) disease has not been assessed. | 2010 | 21177308 |
| synergistic effects of ultraviolet a/riboflavin and glucose on corneal collagen cross-linking. | to investigate the possible synergistic effect of ultraviolet a (uva)/riboflavin and glucose on corneal collagen cross-linking (cxl) by means of the mechanical properties and thermal shrinkage temperatures. | 2010 | 21188957 |
| high frequency of development of b cell lymphoproliferation and diffuse large b cell lymphoma in dbl knock-in mice. | dbl is the prototype of a large family of gdp-gtp exchange factors for small gtpases of the rho family. in vitro, dbl is known to activate rho, rac, and cdc42 and to induce a transformed phenotype in murine fibroblasts. we previously reported that dbl-null mice are viable and fertile but display defective dendrite elongation of distinct subpopulations of cortical neurons, suggesting a role of dbl in controlling dendritic growth. to gain deeper insights into the role of dbl in development and dis ... | 2011 | 21221514 |
| isolation and complete nucleotide sequence of the measles virus imb-1 strain in china. | the complete nucleotide sequence of the measles virus strain imb-1, which was isolated in china, was determined. as in other measles viruses, its genome is 15,894 nucleotides in length and encodes six proteins. the full-length nucleotide sequence of the imb-1 isolate differed from vaccine strains (including wild-type edmonston strain) by 4%-5% at the nucleotide sequence level. this isolate has amino acid variations over the full genome, including in the hemagglutinin and fusion genes. this repor ... | 2010 | 21221916 |
| [increase in glucoamylase productivity of aspergillus awamori strain by combination of radiating mutagenesis and plasmid transformation methods]. | increase in the level of amylolytic genes activator protein encoded by amyr gene was shown to result in enhancement of glucoamylase productivity of a. awamori strain by 30%. however, the same effect equal to 30% increase can be achieved by introduction of extra copies of gla gene encoding glucoamylase. these two effects were not additive, which gave the possibility to suggest an additional limitation in the egulation mechanism of glucoamylase gene expression in aspergillus family strains while i ... | 2010 | 21261079 |
| evolution of major milk proteins in mus musculus and mus spretus mouse species: a genoproteomic analysis. | due to their high level of genotypic and phenotypic variability, mus spretus strains were introduced in laboratories to investigate the genetic determinism of complex phenotypes including quantitative trait loci. mus spretus diverged from mus musculus around 2.5 million years ago and exhibits on average a single nucleotide polymorphism (snp) in every 100 base pairs when compared with any of the classical laboratory strains. a genoproteomic approach was used to assess polymorphism of the major mi ... | 2011 | 21276224 |
| identification and functional characterization of small non-coding rnas in xanthomonas oryzae pathovar oryzae. | small non-coding rnas (srnas) are regarded as important regulators in prokaryotes and play essential roles in diverse cellular processes. xanthomonas oryzae pathovar oryzae (xoo) is an important plant pathogenic bacterium which causes serious bacterial blight of rice. however, little is known about the number, genomic distribution and biological functions of srnas in xoo. | 2011 | 21276262 |
| lactate dehydrogenase a promotes communication between carbohydrate catabolism and virulence in bacillus cereus. | the diarrheal potential of a bacillus cereus strain is essentially dictated by the amount of secreted nonhemolytic enterotoxin (nhe). expression of genes encoding nhe is regulated by several factors, including the metabolic state of the cells. to identify metabolic sensors that could promote communication between central metabolism and nhe expression, we compared four strains of the b. cereus group in terms of metabolic and nhe expression capacities. we performed growth performance measurements, ... | 2011 | 21296961 |
| influence of the lipopolysaccharide structure of salmonella enterica serovar enteritidis on interactions with pig neutrophils. | the key process for immune response development is the recognition of bacteria by the immune system of the host based on the sensing of pathogen-associated molecular patterns (pamp). one of the most important pamp is the lipopolysaccharide (lps) molecule, a complex molecule present in the outer membrane of gram negative bacteria. in this study we were interested in how different parts of the lps of salmonella enterica serovar enteritidis are recognized by porcine neutrophils. to this aim, we con ... | 2011 | 21300486 |
| immunisation with recombinant pfemp1 domains elicits functional rosette-inhibiting and phagocytosis-inducing antibodies to plasmodium falciparum. | rosetting is a plasmodium falciparum virulence factor implicated in the pathogenesis of life-threatening malaria. rosetting occurs when parasite-derived p. falciparum erythrocyte membrane protein one (pfemp1) on the surface of infected erythrocytes binds to human receptors on uninfected erythrocytes. pfemp1 is a possible target for a vaccine to induce antibodies to inhibit rosetting and prevent severe malaria. | 2011 | 21305024 |
| aldose reductase-mediated induction of epithelium-to-mesenchymal transition (emt) in lens. | cataract is a key factor in the morbidity associated with diabetes. while the pathogenesis of diabetic cataract formation is poorly understood, previous research has identified aldose reductase (alr2) as a key player. to elucidate a potential role for this enzyme in diabetic cataract formation, we created a series of transgenic mice designed for expression of human alr2 (akr1b1) in epithelial and outer cortical fiber cells of the lens. one of the founder lines, designated par39, developed an ear ... | 2011 | 21329682 |
| continuous control of the flow in biochemical pathways through 5' untranslated region sequence modifications in mrna expressed from the broad-host-range promoter pm. | the inducible pm promoter integrated into broad-host-range plasmid rk2 replicons can be fine-tuned continuously between the uninduced and maximally induced levels by varying the inducer concentrations. to lower the uninduced background level while still maintaining the inducibility for applications in, for example, metabolic engineering and synthetic (systems) biology, we report here the use of mutations in the pm dna region corresponding to the 5' untranslated region of mrna (utr). five utr var ... | 2011 | 21335387 |
| inhibition of severe acute respiratory syndrome coronavirus replication in a lethal sars-cov balb/c mouse model by stinging nettle lectin, urtica dioica agglutinin. | urtica dioica agglutinin (uda) is a small plant monomeric lectin, 8.7 kda in size, with an n-acetylglucosamine specificity that inhibits viruses from nidovirales in vitro. in the current study, we first examined the efficacy of uda on the replication of different sars-cov strains in vero 76 cells. uda inhibited virus replication in a dose-dependent manner and reduced virus yields of the urbani strain by 90% at 1.1 ± 0.4 µg/ml in vero 76 cells. then, uda was tested for efficacy in a lethal sars-c ... | 2011 | 21338626 |
| comparative genomics of lactobacillus sakei with emphasis on strains from meat. | lactobacillus sakei is a lactic acid bacterium important in food microbiology mainly due to its ability to ferment and preserve meat. the genome sequence of l. sakei strain 23k has revealed specialized metabolic capacities that reflect the bacterium's adaption to meat products, and that differentiate it from other lab. an extensive genomic diversity analysis was conducted to elucidate the core features of the species, and to provide a better comprehension of niche adaptation of the organism. her ... | 2011 | 21369871 |
| a synthetic dna and fusion pcr approach to the ectopic expression of high levels of the d1 protein of photosystem ii in synechocystis sp. pcc 6803. | a hybrid approach involving synthetic dna, fusion pcr, and ectopic expression has been used to genetically manipulate the expression of the d1 protein of photosystem ii (psii) in the model cyanobacterium synechocystis sp. pcc6803. due to the toxicity of the full-length psba gene in e. coli, a chimeric psba2 gene locus was commercially synthesised and cloned in two halves. high-fidelity fusion pcr utilizing sequence overlap between the two synthetic gene halves allowed the production of a dna fra ... | 2011 | 21377372 |
| enhanced thermotolerance for ethanol fermentation of saccharomyces cerevisiae strain by overexpression of the gene coding for trehalose-6-phosphate synthase. | the effect of overexpression of the trehalose-6-phosphate (t6p) synthase gene (tps1) on ethanol fermentation of saccharomyces cerevisiae has been studied at 30 and 38-¦c. the activity of t6p synthase and the accumulation of trehalose during ethanol fermentation were significantly improved by overexpression of tps1, and especially at 38-¦c. ethanol produced by transformants with and without tps1 gene overexpression at 38-¦c was approx. 60 and 37-ág/l, respectively. the fermentation efficiency of ... | 2011 | 21380777 |
| b cell-deficient mice display markedly enhanced resistance to the intracellular bacterium brucella abortus. | brucella species are facultative intracellular bacteria that cause lifelong infections in humans and livestock. | 2011 | 21451002 |
| characterization of a circulating prrsv strain by means of random pcr cloning and full genome sequencing. | abstract: prrs is a pig disease of major economic importance that causes respiratory and reproductive problems in pigs. over the last years it has become clear that prrsv heterogeneity is increasing. consequently, this has a potential impact on diagnosis and strategies to counter this disease. the use of sequence-independent pcr techniques for the detection and characterization of prrsv could be useful to bypass problems associated with the heterogeneity of this virus.a random pcr cloning approa ... | 2011 | 21477345 |
| the major autolysin of streptococcus gordonii is subject to complex regulation and modulates stress tolerance, biofilm formation, and extracellular-dna release. | a gene, designated atls, encoding a major autolysin from streptococcus gordonii, was identified and characterized. the predicted atls protein is 1,160 amino acids and 127 kda and has a conserved β1,4-n-acetylmuramidase domain. zymographic analysis of wild-type s. gordonii revealed peptidoglycan hydrolase activities with molecular masses of 130 and 90 kda that were absent in an atls deletion mutant. western blotting revealed that the 90-kda band was derived from the 130-kda protein. inactivation ... | 2011 | 21478346 |
| biological characterization of russian mycoplasma gallisepticum field isolates. | an earlier study on commercial chickens and turkeys with a history of respiratory disease established mycoplasma gallisepticum infection rates on 164 poultry farms of the russian federation. forty-seven (29%) of these poultry farms were m. gallisepticum-positive by polymerase chain reaction but isolation of the mycoplasma was successful only on 10 farms. five field isolates from different farms were selected for pathogenicity studies in specific pathogen-free chicks. clinical signs, seroconversi ... | 2011 | 21500042 |
| molecular characterization of lapinized classical swine fever vaccine strain by full-length genome sequencing and analysis. | the complete genome of a lapinized classical swine fever virus (csfv) vaccine strain was amplified into nine overlapping fragments by rt-pcr, and nucleotide sequences were determined. complete genome sequence alignment and phylogenetic analysis indicated 92.6-98.6% identities at the nucleotide level with other reported csfv strains and could be grouped into subgroup 1.1 along with other attenuated strains of csfv. the 5'-utr demonstrated >97.0% nucleotide similarity with most of vaccine csfv str ... | 2011 | 21500112 |
| activation of the immune response against infectious bursal disease virus after intramuscular inoculation of an intermediate strain. | infectious bursal disesase is a highly contagious, wide spread immunosuppressive chicken disease caused by the infectious bursal disease virus (ibdv). ibdv is a two segmented double-strand rna virus, member of the birnaviridae family. in order to study the interaction between ibdv and the immune system, chickens were exposed to an intermediate ibdv strain by intramuscular route, and using real time pcr the expression of a panel of avian cytokines and chemokines in duodenum, spleen and bursa of f ... | 2011 | 21514000 |
| invited review: are adaptations present to support dairy cattle productivity in warm climates? | environmental heat stress, present during warm seasons and warm episodes, severely impairs dairy cattle performance, particularly in warmer climates. it is widely viewed that warm climate breeds (zebu and sanga cattle) are adapted to the climate in which they evolved. such adaptations might be exploited for increasing cattle productivity in warm climates and decrease the effect of warm periods in cooler climates. the literature was reviewed for presence of such adaptations. evidence is clear for ... | 2011 | 21524505 |
| post-exposure therapy of inhalational anthrax in the common marmoset. | the aim of this study was to compare the pharmacokinetics and efficacy of ciprofloxacin as post-exposure therapy against inhalational anthrax in the common marmoset (callithrix jacchus) with other non-human primate models in order to determine whether the marmoset is a suitable model to test post-exposure therapies for anthrax. pharmacokinetic (pk) and efficacy studies with ciprofloxacin were performed in the marmoset. ciprofloxacin plasma pharmacokinetics were determined in six animals in separ ... | 2011 | 21530184 |
| co-evolutionary analysis enables rational deregulation of allosteric enzyme inhibition in corynebacterium glutamicum for lysine production. | product feedback inhibition of allosteric enzymes is an essential issue for developing highly efficient microbial strains for bioproduction. here we use aspartokinase from corynebacterium glutamicum (cgak), a key enzyme controlling the biosynthesis of industrially important aspartate family amino acids, as a model to demonstrate a fast and efficient approach to deregulate allostery. in the last fifty years many researchers and companies have made considerable efforts to deregulate this enzyme fr ... | 2011 | 21531824 |
| subtractive hybridization analysis of gastric diseases-associated helicobacter pylori identifies peptidyl-prolyl isomerase as a potential marker for gastric cancer. | helicobacter pylori, a microaerophilic gram-negative bacterium, is known to cause chronic gastritis, peptic ulcer and gastric cancer. genes that are present in certain isolates may determine strain-specific traits such as disease association and drug resistance. in order to understand the pathogenic mechanisms of gastric diseases, identify molecular markers of the diseases associated with h. pylori strains and provide clues for target treatment of h. pylori-related diseases, a subtracted dna lib ... | 2011 | 21535099 |
| generation of a listeria vaccine strain by enhanced caspase-1 activation. | the immunostimulatory properties conferred by vaccine adjuvants require caspase-1 for processing of il-1â and il-18. caspase-1 is activated in response to a breach of the cytosolic compartment by microbes and the process is initiated by intracellular pattern recognition receptors within inflammasomes. listeria monocytogenes is detected in the cytosol by the nlrc4, nlrp3 and aim2 inflammasomes. nlrc4 is activated by flagellin, and l. monocytogenes evades nlrc4 by repressing flagellin expression. ... | 2011 | 21538346 |
| a mechanism for extremely weak spap-expression in streptococcus mutans strain z1. | streptococcus mutans surface-protein antigen (spap, pac, or antigen i/ii) has been well known to play an important role in initial attachment to tooth surfaces. however, strains with weak spap-expression were recently reported to be found in natural populations of s. mutans. the s. mutans gbpc-negative strain z1, which we previously isolated from saliva and plaque samples, apparently expresses relatively low levels of spap protein compared to s. mutans strains mt8148 or ua159. | 2011 | 21541094 |
| cloning and expression of highly pathogenic avian influenza virus full-length nonstructural gene in pichia pastoris. | avian influenza (ai) is a highly contagious and rapidly evolving pathogen of major concern to the poultry industry and human health. rapid and accurate detection of avian influenza virus is a necessary tool for control of outbreaks and surveillance. the ai virus a/chicken/malaysia/5858/2004 (h5n1) was used as a template to produce dna clones of the full-length ns1 genes via reverse transcriptase synthesis of cdna by pcr amplification of the ns1 region. products were cloned into pcr2.0 topo ta pl ... | 2011 | 21541235 |
| mechanical loading-related changes in osteocyte sclerostin expression in mice are more closely associated with the subsequent osteogenic response than the peak strains engendered. | osteocyte sclerostin is regulated by loading and disuse in mouse tibiae but is more closely related to subsequent local osteogenesis than the peak strains engendered. introduction: the purpose of this study was to assess the relationship between loading-related change in osteocyte sclerostin expression, local strain magnitude, and local bone modeling/remodeling. methods: the right tibiae of 19-week-old female c57bl/6 mice were subjected to non-invasive, dynamic axial loading and/or to sciatic ne ... | 2011 | 21573880 |
| protection from persistent infection with a bovine viral diarrhea virus (bvdv) type 1b strain by a modified-live vaccine containing bvdv types 1a and 2, infectious bovine rhinotracheitis virus, parainfluenza 3 virus and bovine respiratory syncytial virus. | recent studies showed that bvdv-1b subgenotype is dominant in north and south american field bvdv isolates. however, nearly all commercially available bvdv-1 vaccines contain bvdv-1a strains. in order to study the efficacy of bvdv-1a vaccine against bvdv-1b infection, this study was designed to evaluate a modified-live vaccine (mlv) containing bvdv-1a and bvdv-2 strains for its efficacy in prevention of persistent infection of fetuses against bvdv-1b strain, when the heifers were vaccinated prio ... | 2011 | 21596076 |
| phylogenetic analysis of puumala virus subtype bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein. | puumala virus (puuv) is the predominant hantavirus species in germany causing large numbers of mild to moderate cases of haemorrhagic fever with renal syndrome (hfrs). during an outbreak in south-east germany in 2004 a novel puuv subtype designated bavaria was identified as the causative agent of hfrs in humans [1]. here we present a molecular characterization of this puuv strain by investigating novel partial and almost entire nucleocapsid (n) protein-encoding small (s-) segment sequences and p ... | 2011 | 21598005 |
| within plant distribution of potato virus y in hairy nightshade (solanum sarrachoides): an inoculum source affecting pvy aphid transmission. | potato virus y (pvy) is vectored by several potato-colonizing and non-colonizing aphid species in a non-persistent manner and has a wide host range. it occurs naturally in several plant families. myzus persicae and macrosiphum euphorbiae are the most efficient potato-colonizing aphid vectors of pvy. rhopalosiphum padi, a cereal aphid that migrates in large numbers through potato fields during the middle of the growing season, does not colonize potato plants but can transmit pvy. hairy nightshade ... | 2011 | 21601597 |
| establishment of an in vitro cell model system to study human prostate carcinogenesis. | a positive family history of prostate cancer is a risk factor for this disease, suggesting that alterations of certain genes may play an important role in the development and progression of prostate cancer. however, genetic alterations responsible for initiation and acquisition of metastatic phenotypes by prostate cancer are not well defined. we have observed a consistent change in chromosome 5 in an in vitro cell model of human prostate carcinogenesis in which the near-diploid cells from the su ... | 1996 | 21544441 |
| iso-migrastatin titer improvement in the engineered streptomyces lividans sb11002 strain by optimization of fermentation conditions. | the heterologous production of iso-migrastatin (iso-mgs) was successfully demonstrated in an engineered s. lividans sb11002 strain, which was derived from s. lividans k4-114, following introduction of pbs11001, which harbored the entire mgs biosynthetic gene cluster. however, under similar fermentation conditions, the iso-mgs titer in the engineered strain was significantly lower than that in the native producer - streptomyces platensis nrrl 18993. to circumvent the problem of low iso-mgs titers ... | 2010 | 21625393 |
| a definition for influenza pandemics based on historial records. | objective: to analyse the records of past influenza outbreaks to determine a definition for pandemics. methods: analysis of publications of large outbreaks of influenza which have occurred since 1889/90, and to match the results against the current definitions of an influenza pandemic. results: according to the general understanding of a pandemic, nine outbreaks of influenza since 1889/90 satisfy the definition; however, for two of these, occurring in 1900 and 1933, the data are limited. the spe ... | 2011 | 21632115 |
| synthesis and characterization of chimeric proteins based on cellulase and xylanase from an insect-gut bacterium. | insects living on wood and plants harbor a large variety of bacterial flora in their guts for degrading biomass. we isolated a paenibacillus strain, designated as icgeb2008, from the gut of a cotton bollworm, on the basis of its ability to secrete a variety of plant hydrolyzing enzymes. in this study, we cloned, expressed and characterized two enzymes, β-1,4-endoglucanase (endo5a) and β-1,4-endoxylanase (xyl11d), from the icgeb2008 strain and synthesized recombinant bifunctional enzymes based on ... | 2011 | 21642416 |
| a unique transcription mode of rabies virus high egg passage-flury strain detected in the infected baby hamster kidney-21 cells. | the transcription mode of rabies virus high egg passage-flury (hep) strain was examined and compared with that of evelyn rokitniki abelseth (era) strain by northern blot analysis using rabies virus gene-specific probes. the era strain was shown exclusively to produce monocistronic mrnas in transcription. while all combinations of multicisronic transcripts including five monocistronic mrnas were detected in the viral rna transcripts of hep strain. it was concluded that the unique transcription mo ... | 2011 | 21645051 |
| monitoring horizontal antibiotic resistance gene transfer in a colonic fermentation model. | the human microbiota is suggested to be a reservoir of antibiotic resistance (abr) genes, which are exchangeable between transient colonizers and residing bacteria. in this study, the transfer of abr genes from enterococcus faecalis to listeria monocytogenes and to commensal bacteria of the human gut microbiota was demonstrated in a colonic fermentation model. in the first fermentation, an e. faecalis donor harboring the marked 50-kb conjugative plasmid pre25(*) and a chromosomal marker was co-i ... | 2011 | 21658089 |
| multistate outbreak of listeria monocytogenes associated with mexican-style cheese made from pasteurized milk among pregnant, hispanic women. | listeriosis is a severe infection caused by listeria monocytogenes. since 2004, the centers for disease control and prevention has requested that listeriosis patients be interviewed using a standardized listeria initiative (li) questionnaire. in january 2009, states and the centers for disease control and prevention began investigating a multistate outbreak of listeriosis among pregnant, hispanic women. we defined a case as an illness occurring between october 2008 and march 2009 with an l. mono ... | 2011 | 21669072 |
| enhanced squalene production by wild-type saccharomyces cerevisiae srains using safe chemical means. | increasing is the interest in establishing renewable sources for squalene, a functional lipid, as the conventional ones are limited. in the present study, squalene production was achieved in wild-type laboratory saccharomyces cerevisiae strain by two safe chemical means, terbinafine (0.05 - 0.55 mm) and methyl jasmonate (mj) (0-1.00 mm). bioprocess kinetics optimized by response surface methodology and monitored by hplc revealed clear dependence of growth, squalene content (sqc) and yield (sqy) ... | 2011 | 21806066 |
| mechanisms controlling virulence thresholds of mixed viral populations. | the propensity of rna viruses to revert attenuating mutations contributes to disease and complicates vaccine development. despite the presence of virulent revertant viruses in some live attenuated vaccines, disease from vaccination is rare. this suggests that in mixed viral populations attenuated viruses may limit pathogenesis of virulent viruses, thus establishing a virulence threshold. here we examined virulence thresholds using mixtures of virulent and attenuated viruses in a transgenic mouse ... | 2011 | 21795346 |
| comparison of real-time pcr with conventional pcr and culture to assess the efficacy of a live attenuated salmonella enterica serovar typhimurium vaccine against salmonella enterica serovar enteritidis in commercial leghorn chicks vaccinated under field and laboratory conditions. | the efficacy of a live attenuated salmonella typhimurium megan vac 1 vaccine (mv1) was evaluated against salmonella enteritidis in chicken pullets with the use of pcr and culture methods. two hundred hyline w-32 white leghorn chicks were obtained from a local hatchery and divided into four treatment groups. two of the groups served as positive and negative controls. the mv1 vaccine was administered to the chicks in the remaining two groups at 1 and 35 days old by either the coarse spray (field) ... | 2011 | 21793441 |
| auxotrophic mutant of staphylococcus aureus interferes with nasal colonization by the wild type. | staphylococcus aureus nasal carriage is a risk factor for infection in humans, particularly in the hospital setting. bacterial interference was used as an alternative strategy for the prevention of upper respiratory, urogenital and gastrointestinal tract infections. this study was designed to assess if the administration of a live-attenuated aroa mutant of s. aureus is useful as a potential approach to prevent transient staphylococcal nasal carriage by virulent strains. we constructed an aroa mu ... | 2011 | 21784166 |
| cytokine profile in murine toxoplasmosis. | to investigate which cytokines are produced after acute infection of mice with toxoplasma gondii (t. gondii) rh strain. | 2011 | 21771408 |
| analysis of the resistome of a multidrug-resistant ndm-1-producing escherichia coli by high-throughput genome sequencing. | the resistome of the multidrug-resistant escherichia coli strain 271 carrying the plasmid-mediated carbapenemase gene bla(ndm-1) was analyzed by high throughput genome sequencing. the p271a plasmid carrying the bla(ndm-1) gene was 35.9 kb in-size and possessed an incn-type backbone that harbored a novel replicase gene. acquisition of the bla(ndm-1) gene on plasmid p271a had been likely the result of a cointegration event involving the transposase of tn5403. the expression of bla(ndm-1) was assoc ... | 2011 | 21746951 |
| packaging signals in the 5'-ends of influenza virus pa, pb1, and pb2 genes as potential targets to develop nucleic-acid based antiviral molecules. | in a previous study a 15-mer phosphorothioate oligonucleotide (s-on) derived from the packaging signal in the 5' end of segment 1 (pb2) of influenza a virus (designated 5-15b) proved markedly inhibitory to virus replication. here we investigated whether analogous inhibitory s-ons targeting the 5' end of segments 2 (pb1) and 3 (pa) could be identified and whether viral resistance to s-ons can be developed. similar to our earlier result, 20-mer s-ons reproducing the 5' ends of segments 2 or 3 (com ... | 2011 | 21741410 |
| inactivation of the extracytoplasmic function sigma factor sig6 stimulates avermectin production in streptomyces avermitilis. | the role of the extracytoplasmic function (ecf) ¤â factor sig6 (sav663) in avermectin production by streptomyces avermitilis was investigated by gene-deletion, complementation and over-expression experiments. inactivation of sig6 had no major effect on growth, stress responses, or morphology. avermectin yield was increased 2- to 2.7-fold (~680-á++g/ml) relative to the wild-type strain by deletion of the sig6 gene, and was restored to the wild-type level by introduction of a single copy of sig6. ... | 2011 | 21691838 |
| creation of a cellooligosaccharide-assimilating escherichia coli strain by displaying active beta-glucosidase on the cell surface via a novel anchor protein. | we demonstrated direct assimilation of cellooligosaccharide using escherichia coli displaying beta-glucosidase (bgl). bgl from thermobifida fusca yx (tfu0937) was displayed on the e. coli cell surface using a novel anchor protein named blc. this strain was grown successfully on 0.2% cellobiose, and the optical density at 600 nm (od(600)) was 1.05 after 20 h. | 2011 | 21742905 |
| preliminary investigation of a mice model of klebsiella pneumoniae subsp. ozaenae induced pneumonia. | in the present study, we comparatively assessed the pathophysiological mechanisms developed during lung infection of balb/c female mice infected by an original wild type klebsiella pneumoniae subsp. ozaenae strain (ch137) or by a referent subspecies k. pneumoniae. subsp. pneumoniae strain (atcc10031). the mice infected with 2.10(6)-ácfu k. p. subsp. pneumoniae (n-á=-á10) showed transient signs of infection and all of them recovered. all of those infected with 1.10(6)-ácfu k. p. subsp. ozaenae (n ... | 2011 | 21723409 |
| reduced airborne transmission of oseltamivir-resistant pandemic a/h1n1 virus in ferrets. | the h275y neuraminidase mutation conferring oseltamivir resistance has been reported in several pandemic a/h1n1 (ph1n1) isolates. we sought to evaluate transmission of this mutant virus through the direct contact and the airborne (aerosol and droplet) routes in the ferret model. | 2011 | 21817200 |
| mapping genetic loci that interact with myostatin to affect growth traits. | myostatin, or gdf8, is an inhibitor of skeletal muscle growth. a non-functional myostatin mutation leads to a double muscling phenotype in some species, for example, mice, cattle and humans. previous studies have indicated that there are loci in the genome that interact with myostatin to control backfat depth and other complex traits. we now report a quantitative trait loci (qtl) mapping study designed to identify loci that interact with myostatin to impact growth traits in mice. body weight and ... | 2011 | 21694733 |
| the context preexposure facilitation effect in mice: a dose-response analysis of pretraining scopolamine administration. | the context preexposure facilitation effect (cpfe) is an elaboration of contextual fear conditioning and refers to enhanced contextual conditioning resulting from preexposure to the context prior to a separate, brief context-shock episode. a version of the cpfe developed by rudy and colleagues in rats has demonstrated greater sensitivity to pre-training hippocampal insult relative to standard contextual fear conditioning preparations. our aim was to adapt the rudy cpfe procedures to mice. in exp ... | 2011 | 21827794 |
| prevalence of plasmid-mediated quinolone resistance genes in uropathogenic escherichia coli isolated in a teaching hospital of northern italy. | a retrospective study was conducted to determine the prevalence of plasmid-mediated quinolone resistance (pmqr) determinants in uropathogenic escherichia coli isolated from inpatients and outpatients in a teaching hospital of northern italy. the presence of qnra, qnrb, qnrs, aac(6')-ib-cr, and qepa was evaluated in 76 and 72 nalidixic acid-resistant e. coli, isolated in 2004 and 2006, respectively. positivity for the aac(6')-ib-cr gene was demonstrated in 3 of 76 (3.9%) and 8 of 72 (11%) isolate ... | 2011 | 21711147 |
| elicitation of anti-1918 influenza virus immunity early in life prevents morbidity and lower levels of lung infection by 2009 pandemic h1n1 influenza virus in aged mice. | the spanish influenza virus pandemic of 1918 was responsible for 40 million to 50 million deaths and is antigenically similar to the swine lineage 2009 pandemic influenza virus. emergence of the 2009 pandemic from swine into humans has raised the possibility that low levels of cross-protective immunity to past shared epitopes could confer protection. in this study, influenza viruslike particles (vlps) were engineered to express the hemagglutinin (ha) and genes from the 1918 influenza virus to ev ... | 2011 | 22130546 |
| methods of evaluating protective clothing relative to heat and cold stress: thermal manikin, biomedical modeling, and human testing. | personal protective equipment (ppe) refers to clothing and equipment designed to protect individuals from chemical, biological, radiological, nuclear, and explosive hazards. the materials used to provide this protection may exacerbate thermal strain by limiting heat and water vapor transfer. any new ppe must therefore be evaluated to ensure that it poses no greater thermal strain than the current standard for the same level of hazard protection. this review describes how such evaluations are typ ... | 2011 | 21936698 |
| The 2009 pandemic H1N1 D222G hemagglutinin mutation alters receptor specificity and increases virulence in mice but not in ferrets. | The D222G (H1 numbering) hemagglutinin (HA) mutation within the receptor-binding site was detected with higher frequencies in severe cases of 2009 pandemic H1N1 (pH1N1) infections. We investigated the impact of this mutation in vitro and in animal models using recombinant pH1N1 viruses. | 2011 | 21881115 |
| Infection with Seasonal Influenza Virus Elicits CD4 T Cells Specific for Genetically Conserved Epitopes That Can Be Rapidly Mobilized for Protective Immunity to Pandemic H1N1 Influenza Virus. | In recent years, influenza viruses with pandemic potential have been a major concern worldwide. One unresolved issue is how infection or vaccination with seasonal influenza virus strains influences the ability to mount a protective immune response to novel pandemic strains. In this study, we developed a mouse model of primary and secondary influenza infection by using a widely circulating seasonal H1N1 virus and the pandemic strain of H1N1 that emerged in Mexico in 2009, and we evaluated several ... | 2011 | 21976658 |
| A duck enteritis virus-vectored bivalent live vaccine provides fast and complete protection against H5N1 avian influenza virus infection in ducks. | Ducks play an important role in the maintenance of highly pathogenic H5N1 avian influenza viruses (AIVs) in nature, and the successful control of AIVs in ducks has important implications for the eradication of the disease in poultry and its prevention in humans. The inactivated influenza vaccine is expensive, labor-intensive, and usually needs 2 to 3 weeks to induce protective immunity in ducks. Live attenuated duck enteritis virus (DEV; a herpesvirus) vaccine is used routinely to control lethal ... | 2011 | 21865383 |
| A Dynamically Cultured Collagen/Cells-Incorporated Elastic Scaffold for Small-Diameter Vascular Grafts. | There is an essential demand for tissue-engineered autologous small-diameter vascular grafts, which offer temporary supports and guides for vascular tissue organization, repair and remodeling. This study reports on the effect of collagen/smooth muscle cells (SMCs) mixtures under dynamic cultures and SMC-endothelial cell (ECs) co-culture on cell proliferation, uniform cell distribution, extracellular matrix deposition, and endothelial cells monolayer formation in tissue-engineered tubular arteria ... | 2011 | 21943800 |
| sequencing of bovine herpesvirus 4 v.test strain reveals important genome features. | bovine herpesvirus 4 (bohv-4) is a useful model for the human pathogenic gammaherpesviruses epstein-barr virus and kaposi's sarcoma-associated herpesvirus. although genome manipulations of this virus have been greatly facilitated by the cloning of the bohv-4 v.test strain as a bacterial artificial chromosome (bac), the lack of a complete genome sequence for this strain limits its experimental use. | 2011 | 21846388 |
| [detection and analysis of gene polymorphism in hepatitis b virus c region]. | objective: to explore the variations of gene c in hepatitis b viruses between hepatitis b patients and healthy carriers, and provide experimental evidences for analysis of virus gene mutations acting on the virus material science and response of the body to the virus.methods: the virus dna load in hepatitis b patients and healthy blood donors was investigated by real-time polymerase chain reaction (pcr). gene sequence analysis was taken to detect gene polymorphism, and all the success samples we ... | 2011 | 22097597 |
| sociability and brain development in balb/cj and c57bl/6j mice. | sociability-the tendency to seek social interaction-propels the development of social cognition and social skills, but is disrupted in autism spectrum disorders (asd). balb/cj and c57bl/6j inbred mouse strains are useful models of low and high levels of juvenile sociability, respectively, but the neurobiological and developmental factors that account for the strains' contrasting sociability levels are largely unknown. we hypothesized that balb/cj mice would show increasing sociability with age b ... | 2011 | 22178318 |
| histomorphometric characteristics of immune cells in small intestine of pigs perorally immunized with vaccine candidate f18ac nonenterotoxigenic e. coli strain. | colidiarrhea and colienterotoxemia caused by f4(+) and/or f18(+) enterotoxigenic e. coli (etec) strains are the most prevalent infections of suckling and weaned pigs. here we tested the immunogenicity and protective effectiveness of attenuated f18ac(+) non-etec vaccine candidate strain against challenge infection with f4ac(+) etec strain by quantitative phenotypic analysis of small intestinal leukocyte subsets in weaned pigs.we also evaluated levamisole as an immune response modifier (irm) and i ... | 2009 | 22073355 |
| induction of albuminuria and kidney damage in shr by transfer of chromosome 8 from munich wistar fromter rats. | inbred munich wistar frömter [mwf/fubrkb (rgd:724569), mwf] rats develop progressive albuminuria with age that is under polygenetic influence. we previously identified a major albuminuria quantitative trait locus (qtl) on rat chromosome (rno)8 in mwf. to test the independent role of qtl(s) for albuminuria development on rno8, we generated a consomic shr-chr 8(mwf)/rkb (shr-8(mwf)) strain by transferring rno8 from mwf into the albuminuria-resistant background of the spontaneously hypertensive rat ... | 2011 | 22108208 |
| Properties of a high malic acid-producing strains of Saccharomyces cerevisiae isolated from sake mash. | We characterized high malic acid-producing strains of Saccharomyces cerevisiae isolated from sake mash. We compared the gene expression of these strains with those of the parental strain by DNA microarray, and found that stress response genes, such as HSP12, were commonly upregulated in the high malate-producing strains, whereas thiamine synthesis genes, such as THI4 and SNZ2, were downregulated in these strains. | 2011 | 21979083 |
| epstein-barr virus induces erosive arthritis in humanized mice. | epstein-barr virus (ebv) has been implicated in the pathogenesis of rheumatoid arthritis (ra) on the basis of indirect evidence, such as its presence in affected joint tissues, antigenic cross reactions between ebv and human proteins, and elevated humoral and cellular anti-ebv immune responses in patients. here we report development of erosive arthritis closely resembling ra in humanized mice inoculated with ebv. human immune system components were reconstituted in mice of the nod/shi-scid/il-2r ... | 2011 | 22028930 |
| mur regulates the gene encoding the manganese transporter mnth in brucella abortus 2308. | mnth is the only high-affinity manganese transporter identified in brucella. a previous study showed that mnth is required for the wild-type virulence of brucella abortus 2308 in mice (anderson es, et al., infect. immun. 77:3466-3474, 2009) and indicated that the mnth gene is regulated in a manganese-responsive manner in this strain by a mur homolog. in the study presented here, the transcriptional start site for mnth in b. abortus 2308 was determined by primer extension analysis. specific inter ... | 2011 | 22101848 |
| ribm from streptomyces davawensis is a riboflavin/roseoflavin transporter and may be useful for the optimization of riboflavin production strains. | the bacterium bacillus subtilis, which is not a natural riboflavin overproducer, has been converted into an excellent production strain by classical mutagenesis and metabolic engineering. to our knowledge, the enhancement of riboflavin excretion from the cytoplasm of overproducing cells has not yet been considered as a target for (further) strain improvement. here we evaluate the flavin transporter ribm from streptomyces davawensis with respect to improvement of a riboflavin production strain. | 2011 | 22136195 |
| infection with conditionally virulent streptococcus pneumoniae δpab strains induces antibody to conserved protein antigens but does not protect against systemic infection with heterologous strains. | avirulent strains of a bacterial pathogen could be useful tools for investigating immunological responses to infection and potentially effective vaccines. we have therefore constructed an auxotrophic tigr4 δpab strain of streptococcus pneumoniae by deleting the pabb gene sp_0665. the tigr4 δpab strain grew well in complete medium but was unable to grow in serum unless it was supplemented with para-aminobenzoic acid (paba). the tigr4 δpab strain was markedly attenuated in virulence in mouse model ... | 2011 | 21947774 |
| ucma is not necessary for normal development of the mouse skeleton. | ucma (upper zone of growth plate and cartilage matrix associated protein) is a highly conserved tyrosine-sulphated secreted protein of mw 17kda, which is expressed by juvenile chondrocytes. to evaluate the physiological function of this novel cartilage protein, we generated a ucma-deficient mouse strain by introducing a lacz/neor-cassette into the first exon of the ucma gene. this mutation results in the complete loss of ucma mrna and protein expression. surprisingly, however, although previous ... | 2011 | 22155508 |
| pseudomonas zeshuii sp. nov., isolated from contaminated soil. | a gram-negative, rod-shaped, non-motile, non-spore-forming bacterium, designated strain by-1t, was isolated from a soil sample from the city of qiqihar in heilongjiang province, china. strain by-1t grew optimally at ph 7.0 and 30-35°c in the presence of 0.5% (w/v) nacl. an analysis of 16s rrna gene sequences revealed that strain by-1t fell within the radiation of the genus pseudomonas, as it showed highest sequence similarities to pseudomonas luteola iam 13000t (99.5 %) and pseudomonas duriflava ... | 2011 | 22199212 |
| comparative analysis of the complete genome sequences of two australian origin live attenuated vaccines of infectious laryngotracheitis virus. | infectious laryngotracheitis virus (iltv) is an alphaherpesvirus that causes acute respiratory disease in poultry. live attenuated iltv vaccines have been used extensively to help control outbreaks of disease. two australian-origin attenuated vaccine strains, sa2 and a20 iltv, are commercially available and are in frequent use in australia. both these vaccines are of chicken embryo origin (ceo). the a20 iltv strain was developed from the sa2 iltv strain by sequential passage of sa2 iltv in tissu ... | 2011 | 22044743 |
| genetic comparison among dolphin morbillivirus in the 1990-1992 and 2006-2008 mediterranean outbreaks. | in 1990, dolphin morbillivirus (dmv) killed thousands of striped dolphins in the mediterranean. subsequently, the prevalence of the infection declined in this species. in 2006-2008, the virus killed not only numerous striped dolphins but also long-finned pilot whales. all partial sequences of the phosphoprotein and nucleoprotein genes obtained thus far from different host species during the 2006-2008 outbreak show 100% identity, suggesting that a single virus was involved, and these sequences ar ... | 2011 | 21888991 |
| methodologies for salmonella enterica subsp. enterica subtyping: gold standards and alternatives. | for more than 80 years, subtyping of salmonella enterica has been routinely performed by serotyping, a method in which surface antigens are identified based on agglutination reactions with specific antibodies. the serotyping scheme, which is continuously updated as new serovars are discovered, has generated over time a data set of the utmost significance, allowing long-term epidemiological surveillance of salmonella in the food chain and in public health control. conceptually, serotyping provide ... | 2011 | 21856826 |
| point mutations in the inc antisense rna gene are associated with increased plasmid copy number, expression of blacmy-2 and resistance to piperacillin/tazobactam in escherichia coli. | high-level expression of ampc β-lactamase genes is associated with increased resistance to β-lactam antibiotics. bla(cmy-2) is the most prevalent plasmid-encoded ampc gene found in escherichia coli worldwide, and the gene is often found on plasmids of the inci1 replicon type. replication of inci1 plasmids is controlled by antisense rna transcribed from the gene inc, and nucleotide changes in the hairpin loop region of inc have been associated with increased plasmid copy number of inci1 mini-plas ... | 2011 | 22117029 |
| SnO(2) Nanowire Logic Devices on Deformable Nonplanar Substrates. | Logic inverters consisting of n-type FETs and resistors with SnO(2) nanowire channels were fabricated on films of the elastomer polydimethylsiloxane, prestrained and flattened into planar sheets from initial, preformed hemispherical shapes. Upon release, thin and narrow interconnects between individual devices in the arrays absorb induced strain by buckling into nonplanar sinusoidal shapes, to allow full recovery of the surfaces to their original convex geometries. The same physics allows deform ... | 2011 | 22084941 |
| Enhanced incorporation of 3-hydroxy-4-methylvalerate unit into biosynthetic polyhydroxyalkanoate using leucine as a precursor. | ABSTRACT: Ralstonia eutropha PHB-4 expressing Pseudomonas sp. 61-3 polyhydroxyalkanoate (PHA) synthase 1 (PhaC1Ps) synthesizes PHA copolymer containing 3-hydroxybutyrate (3HB) and a small amount (0.5 mol%) of 3-hydroxy-4-methylvalerate (3H4MV) from fructose as a carbon source. In this study, enhanced incorporation of 3H4MV into PHA was investigated using branched amino acid leucine as a precursor of 3H4MV. Leucine has the same carbon backbone as 3H4MV and is expected to be a natural and self-pro ... | 2011 | 21906338 |
| first report of a trichinella papuae infection in a wild pig (sus scrofa) from an australian island in the torres strait region. | multiple trichinella species are reported from the australasian region although mainland australia has never confirmed an indigenous case of trichinella infection in humans or animals. wildlife surveys in high-risk regions are essential to truly determine the presence or absence of trichinella, but in mainland australia are largely lacking. in this study, a survey was conducted in wild pigs from mainland australia's cape york peninsula and torres strait region for the presence of trichinella, gi ... | 2011 | 22030374 |
| overexpression of (his)6-tagged human arginase i in saccharomyces cerevisiae and enzyme purification using metal affinity chromatography. | arginase (ec 3.5.3.1; l-arginine amidinohydrolase) is a key enzyme of the urea cycle that catalyses the conversion of arginine to ornithine and urea, which is the final cytosolic reaction of urea formation in the mammalian liver. the recombinant strain of the yeast saccharomyces cerevisiae that is capable of overproducing arginase i (rharg1) from human liver under the control of the efficient copper-inducible promoter cup1, was constructed. the (his)(6)-tagged rharg1 was purified in one step fro ... | 2012 | 21945700 |
| rapid identification and validation of specific molecular targets for detection of escherichia coli o104:h4 outbreak strain by use of high-throughput sequencing data from nine genomes. | | 2011 | 21880963 |
| impact of spores on the comparative efficacies of five antibiotics for the treatment of bacillus anthracis in an in vitro hollow fiber pharmacodynamic model. | background: bacillus anthracis (ba), the bacterium that causes anthrax, is an agent of bioterrorism. the most effective antimicrobial therapy for ba infections is unknown. methods: an in vitro pharmacodynamic model of ba was used to compare the efficacies of simulated clinically-prescribed regimens of moxifloxacin, linezolid, and meropenem with the "gold standards," doxycycline and ciprofloxacin. treatment outcomes for isogenic spore-forming and non-spore-forming strains of ba were compared. res ... | 2011 | 22155821 |
| Metabolic Engineering of Nocardia sp. CS682 for Enhanced Production of Nargenicin A(1). | A number of secondary metabolites having therapeutic importance have been reported from the genus Nocardia. One of the polyketide antibiotic compounds isolated from Nocardia is nargenicin A(1). Recently, nargenicin A(1) has been isolated from Nocardia sp. CS682, a new Nocardia strain isolated from soil in Jeonnam, South Korea. It possesses strong antibacterial activity against methicillin-resistant Staphylococcus aureus. In this study, we applied a metabolic engineering approach based on recombi ... | 2011 | 22161261 |