| effect of cryopreservation by slow cooling and vitrification on viral contamination of ivf embryos experimentally exposed to bovine viral diarrhea virus and bovine herpesvirus-1. | the objective was to determine the effect of cryopreservation by conventional slow controlled cooling (0.5 degrees c/min) and by vitrification on the presence of bovine viral diarrhea virus (bvdv) and bovine herpesvirus-1 (bhv-1) infectivity associated with frozen-thawed day 7 bovine embryos. in this study, day 7 embryos generated by in vitro fertilization (ivf) were exposed in vitro for 1.5h to bvdv (n=393) and bhv-1 (n=242) and subsequently tested before and after cryopreservation for the pres ... | 2009 | 19616294 |
| infertility and venereal disease in cattle inseminated with semen containing bovine herpesvirus type 5. | a group of 20 cows and heifers experienced poor conception rates and probable ovarian dysfunction after being artificially inseminated. when first examined, some showed signs of vulvovaginitis, with pustular, ulcerative lesions consistent with a herpesvirus infection. they had had no contact with bulls during the current breeding season. bovine herpesvirus type 5 (bhv-5) was isolated from samples of frozen semen from the batch that had been used for the artificial insemination programme. bhv-5 w ... | 2009 | 19633324 |
| repertoire of bovine mirna and mirna-like small regulatory rnas expressed upon viral infection. | microrna (mirna) and other types of small regulatory rnas play a crucial role in the regulation of gene expression in eukaryotes. several distinct classes of small regulatory rnas have been discovered in recent years. to extend the repertoire of small rnas characterized in mammals and to examine relationship between host mirna expression and viral infection we used illumina's ultrahigh throughput sequencing approach. we sequenced three small rna libraries prepared from cell line derived from the ... | 2009 | 19633723 |
| [construction and identification of recombinant bhv-1 expressing foot and mouth disease virus vp1 gene]. | in order to construct the recombinant bovine hepervirus-1 (bhv-1) which expressed foot and mouth disease virus (fmdv) vp1 gene, we constructed a bhv-1 ge gene transfer vector by inserting the synthetic vp1 gene of fmdv (o/china/99) under the immediate-early promoter of cytomegalovirus. | 2009 | 19637579 |
| prevalence of brucellosis and infectious bovine rhinotracheitis in organized dairy farms in india. | this study was carried out to investigate the prevalence of bovine brucellosis and infectious bovine rhinotracheitis (ibr) in organized dairy farms with history of abortion in india. elisa and rose bengal plate test (rbpt) were used to detect the seropositive animals and the test results indicated that 22.18% and 13.78% animals were declared as sero-positive by elisa and rbpt, respectively. milk ring test (mrt) was carried out only in one farm and 12.82% of the tested animals were turned positiv ... | 2010 | 19644761 |
| characterization of the nuclear and nucleolar localization signals of bovine herpesvirus-1 infected cell protein 27. | bovine herpesvirus-1 infected cell protein 27 (bicp27) was detected predominantly in the nucleolus. the open reading frame of bicp27 was fused with the enhanced yellow fluorescent protein (eyfp) gene to investigate its subcellular localization in live cells and bicp27 was able to direct monomeric, dimeric or trimeric eyfp exclusively to the nucleolus. by constructing a series of deletion mutants, the putative nuclear localization signal (nls) and nucleolar localization signal (nols) were mapped ... | 2009 | 19682510 |
| exposure to pathogens and incidence of respiratory disease in young bulls on their arrival at fattening operations in france. | the incidence of clinical respiratory disease in 698 young beef bulls kept in 68 pens, and their exposure to respiratory pathogens after their arrival at 51 fattening operations in western france were assessed. antibodies against bovine respiratory syncytial virus (brsv), bovine herpesvirus type 1 (bhv-1), mannheimia haemolytica and mycoplasma bovis were measured by elisa. the incidence risk of respiratory disease was 18.5 per cent during the first six weeks. cases occurred in 37 of the 68 pens, ... | 2009 | 19684344 |
| seroconversion to bovine viral diarrhoea virus and infectious bovine rhinotracheitis virus in dairy herds of michoacan, mexico. | bovine viral diarrhoea virus (bvdv) and infectious bovine rhinotracheitis virus (ibrv) are important viral diseases around the world. the objective of this study was to estimate the incidence of seroconversion to bvdv and ibrv and to identify associated risk factors in dairy herds of michoacan, mexico. the longitudinal study included 62 herds and ran from december 2001 to november 2002. the total number of animals enrolled and completing the study were 392 and 342 animals for bvdv and 925 and 89 ... | 2010 | 19685278 |
| major tegument protein vp8 of bovine herpesvirus 1 is phosphorylated by viral us3 and cellular ck2 protein kinases. | the ul47 gene product, vp8, is one of the major tegument proteins of bovine herpesvirus 1 (bohv-1) and is subject to phosphorylation. analysis of protein bands co-immunoprecipitated with vp8 from bohv-1-infected cells by mass spectroscopy suggested that vp8 interacts with two protein kinases: cellular ck2 and viral us3. ck2 is a highly conserved cellular protein, expressed ubiquitously and known to phosphorylate numerous proteins. the us3 gene product is one of the viral kinases produced by bohv ... | 2009 | 19692545 |
| bovine herpesvirus: what is missing from our understanding of the relationship between bohv-1 and bohv-5? | | 2010 | 19703787 |
| efficacy of a combination viral vaccine for protection of cattle against experimental infection with field isolates of bovine herpesvirus-1. | to determine whether a combination viral vaccine containing a modified-live bovine herpesvirus-1 (bhv-1) would protect calves from infection with virulent field strains of bhv-1 for weeks or months after vaccination. | 2009 | 19719449 |
| comparison of single vaccination versus revaccination with a modified-live virus vaccine containing bovine herpesvirus-1, bovine viral diarrhea virus (types 1a and 2a), parainfluenza type 3 virus, and bovine respiratory syncytial virus in the prevention of bovine respiratory disease in cattle. | objective-to compare effects of administration of a modified-live respiratory virus vaccine once with administration of the same vaccine twice on the health and performance of cattle. design-randomized, controlled trial. animals-612 mixed-breed male cattle with unknown health histories. procedures-cattle were randomly assigned to 1 of 2 treatment groups (single vaccination treatment group [svac group] vs revaccination treatment group [revac group]) during the preconditioning phase of production. ... | 2009 | 19719452 |
| neutralizing antibody responses in preconditioned calves following vaccination for respiratory viruses. | calf preconditioning programs that include vaccinations against common respiratory pathogens are beneficial; however, optimal vaccination protocols have not been determined. in the study described here, calves were placed in a preconditioning program and assigned to one of nine vaccine regimens, each consisting of some combinations of killed or modified-live virus vaccines against bovine herpesvirus-1 (bhv-1), bovine viral diarrhea virus (bvdv), parainfluenza-3 (pi-3) virus, and bovine respirato ... | 2002 | 19750742 |
| response to modified live and killed multivalent viral vaccine in regularly vaccinated, fresh dairy cows. | vaccination programs for viral pathogens in the dairy industry span the full spectrum of possibilities even though few of these have been evaluated in field situations. one such program is the vaccination of fresh cows 30 to 60 days postpartum with modified live viral (mlv) vaccines. the purpose of this study was to evaluate the antibody response to booster vaccinations during this period. the impact of vaccinations on milk production and reproductive performance was also examined. the response ... | 2000 | 19757565 |
| comparison of bovine herpesvirus 1 vaccines for rapid induction of immunity. | the purpose of the study reported here was to compare four different vaccine regimens for their efficacy in protecting calves from challenge with bovine herpesvirus 1 (bhv1) at 5 and 14 days after vaccination. nine experimental groups of five calves each were used to compare four vaccination regimens and a group that received no vaccine. the four vaccine regimens were: a bhv1 intramuscular (im) vaccine containing both modified live virus (mlv) and killed virus (kv); an mlv intranasal (in) vaccin ... | 2000 | 19757568 |
| characterization of micrornas encoded by the bovine herpesvirus 1 genome. | bovine herpesvirus 1 (bohv-1) is a ubiquitous and important pathogen of cattle worldwide. this study reports the identification of 10 microrna (mirna) genes, bhv1-mir-b1-bhv1-mir-b10, encoded by the bohv-1 genome that were processed into 12 detectable mature mirnas as determined by ultra-high throughput sequencing bioinformatics analyses of small rna libraries and expression studies. we found that four of the mirna genes were present as two copies in the bohv-1 genome, resulting in a total of 14 ... | 2010 | 19793906 |
| cloning of bovine herpesvirus type 1 and type 5 as infectious bacterial artifical chromosomes. | bovine herpesviruses type 1 (bohv1) and type 5 (bohv5) are two closely related pathogens of cattle. the identity of the two viruses on the amino acid level averages 82%. despite their high antigenetic similarities the two pathogens induce distinctive clinical signs. bohv1 causes respiratory and genital tract infections while bohv5 leads to severe encephalitis in calves. | 2009 | 19828032 |
| a ul47 gene deletion mutant of bovine herpesvirus type 1 exhibits impaired growth in cell culture and lack of virulence in cattle. | tegument protein vp8 encoded by the u(l)47 gene of bovine herpesvirus type 1 (bhv-1) is the most abundant constituent of mature virions. in the present report, we describe the characterization of u(l)47 gene-deleted bhv-1 in cultured cells and its natural host. the u(l)47 deletion mutant exhibited reduced plaque size and more than 100-fold decrease in intracellular and extracellular viral titers in cultured cells. ultrastructural observations of infected cells showed normal maturation of bhv-1 v ... | 2010 | 19864376 |
| bovine herpesvirus type 1 as a novel oncolytic virus. | oncolytic virotherapy is a promising avenue of cancer gene therapy. current vectors include human viruses that have been engineered to replicate in tumor cells or nonhuman viruses that are naturally oncotropic and preferentially replicate in tumor cells harboring defects in innate immune pathways such as the type 1 interferon (ifn) pathway. bovine herpesvirus type 1 (bhv-1) is a species-specific herpesvirus closely related to the human herpes simplex virus type 1 (hsv-1). although bhv-1 does not ... | 2010 | 19893594 |
| evaluation of safety and efficacy of dna vaccines against bovine herpesvirus-1 (bohv-1) in calves. | four dna vaccines against bohv-1 were evaluated for their efficacy in calves. twelve animals were divided into four groups which were injected with four different dna vaccines: pvax-tgd (vaccine a); pvax-tgd co-immunised with pvax-48cpg (vaccine b); pvax-ubilaci-tgd-l (vaccine c); pvax-ubilaci-tgd-l co-immunised with pvax-48cpg (vaccine d). three additional calves were given the plasmid vector and served as controls. ninety days after the first vaccination all calves were challenge infected with ... | 2011 | 19906427 |
| intracellular trafficking of vp22 in bovine herpesvirus-1 infected cells. | the intracellular trafficking of different vp22-enhanced yellow fluorescent protein (eyfp) fusion proteins expressed by bovine herpesvirus-1 (bhv-1) recombinants was examined by live-cell imaging. our results demonstrate that (i) the fusion of eyfp to the c terminus of vp22 does not alter the trafficking of the protein in infected cells, (ii) vp22 expressed during bhv-1 infection translocates to the nucleus through three different pathways, namely early mitosis-dependent nuclear translocation, l ... | 2010 | 19922972 |
| neutralizing antibodies to bovine herpesvirus types 1 (bohv-1) and 5 (bohv-5) and its subtypes. | this study was carried out to determine whether the sensitivity of serum neutralization (sn) tests would be affected by the use of distinct subtypes of bovine herpesvirus 1 (bohv-1) and 5 (bohv-5) as test challenge viruses. bovine sera collected from a randomized sample (n=287) were tested in a 24h incubation sn against three type 1 viruses (bohv-1.1 strains "los angeles" (la) and "evi 123"; bohv-1.2a strain "sv 265") and three type 5 viruses (bohv-5a strain "evi 88"; bohv-5b strain "a 663" and ... | 2010 | 19926411 |
| inhibition of bovine herpesvirus multiplication in mdbk cells by small interfering rnas. | small interfering rna (sirna) mediated gene silencing is a promising approach in antiviral therapy. to investigate the antiviral effects of sirnas on bovine herpesvirus 1 (bohv-1) multiplication, we designed and in vitro synthesized two sirnas (sirna-1 and sirna-2) targeting the ul25 gene that is essential for bhv-1 multiplication. sirna-1 and sirna-2 inhibited the bohv-1 multiplication in mdbk cells to a different extent, namely by 11% and 40%, respectively, as demonstrated by virus titers (tci ... | 2009 | 19941403 |
| bovine respiratory disease research (1983-2009). | bovine respiratory disease (brd) research has provided significant understanding of the disease over the past 26 years. modern research tools that have been used include monoclonal antibodies, genomics, polymerase chain reaction, immunohistochemistry (ihc), dna vaccines and viral vectors coding for immunogens. emerging/reemerging viruses and new antigenic strains of viruses and bacteria have been identified. methods of detection and the role for cattle persistently infected bovine viral diarrhea ... | 2009 | 20003649 |
| host response to bovine respiratory pathogens. | bovine respiratory disease (brd) involves complex interactions amongst viral and bacterial pathogens that can lead to intense pulmonary inflammation (fibrinous pleuropneumonia). viral infection greatly increases the susceptibility of cattle to secondary infection of the lung with bacterial pathogens like mannheimia haemolytica and histophilus somni. the underlying reason for this viral/bacterial synergism, and the manner in which cattle respond to the virulence strategies of the bacterial pathog ... | 2009 | 20003650 |
| update on viral pathogenesis in brd. | many viruses, including bovine herpesvirus-1 (bhv-1), bovine respiratory syncytial virus (brsv), parainfluenzavirus-3 (pi3), bovine coronavirus, bovine viral diarrhea virus and bovine reovirus, have been etiologically associated with respiratory disease in cattle. this review focuses on the pathogenesis of bhv-1 and brsv, two very different agents that primarily cause disease in the upper and lower respiratory tract, respectively. | 2009 | 20003652 |
| construction of single-chain fv with two possible cdr3h conformations but similar inter-molecular forces that neutralize bovine herpesvirus 1. | bovine herpesvirus 1 (bohv-1) causes respiratory and genital diseases in cattle for which available vaccines do not confer adequate protection. since passive immunization with antibodies permits disease prevention, single-chain fragment variable (scfv), originating from a monoclonal bovine igg1 antibody against bohv-1, were constructed and expressed in pichia pastoris in v(lambda)-v(h) orientation via a flexible seven-amino acid linker. similar to the intact igg, the purified recombinant scfv ne ... | 2010 | 20006385 |
| bovine herpesvirus-1 us3 protein kinase: critical residues and involvement in the phosphorylation of vp22. | the us3 gene product of bovine herpesvirus-1 (bohv-1) is a protein kinase that is expressed early during infection and capable of autophosphorylation. by examining differentially labelled us3 moieties by co-immunoprecipitation, we demonstrated that the protein kinase interacts with itself in vitro, which supports autophosphorylation by us3. based on its homology to other serine/threonine protein kinases, we defined two highly conserved lysines in us3, at position 195 within the atp-binding pocke ... | 2010 | 20016039 |
| a bovine herpesvirus 5 recombinant defective in the thymidine kinase (tk) gene and a double mutant lacking tk and the glycoprotein e gene are fully attenuated for rabbits. | bovine herpesvirus 5 (bohv-5), the agent of herpetic meningoencephalitis in cattle, is an important pathogen of cattle in south america and several efforts have been made to produce safer and more effective vaccines. in the present study, we investigated in rabbits the virulence of three recombinant viruses constructed from a neurovirulent brazilian bohv-5 strain (sv507/99). the recombinants are defective in glycoprotein e (bohv-5gedelta), thymidine kinase (bohv-5tkdelta) and both proteins (bohv ... | 2010 | 20027480 |
| surveys for disease agents in introduced elk in arkansas and kentucky. | surveys for disease agents were conducted in introduced free-ranging elk (cervus elaphus nelsoni) in arkansas and kentucky. elk had been captured in colorado and nebraska and released in arkansas during 1981-1985. from 1997 through 2002 elk were captured in arizona, kansas, north dakota, new mexico, oregon, and utah and released in southeastern kentucky. specimens were collected from 170 hunter-killed elk in arkansas during 1998-2006, and 44 elk in kentucky during 2001-2004. significant findings ... | 2010 | 20090032 |
| critical role of cholesterol in bovine herpesvirus type 1 infection of mdbk cells. | cholesterol is involved in the life cycle of many viruses. here, we examined the role of cholesterol for both viral envelope and target cell membrane for bovine herpesvirus type 1 (bohv-1) infection. cholesterol depletion by pretreatment of madin-darby bovine kidney (mdbk) cells with a cholesterol-sequestering drug methyl-beta-cyclodextrin (mbetacd), inhibited the production of bohv-1 in a dose-dependent manner. this inhibitory effect was partially reversed by cholesterol replenishment, indicati ... | 2010 | 20097021 |
| comparison of the biological and biochemical activities of several members of the alphaherpesvirus icp0 family of proteins. | immediate-early protein icp0 of herpes simplex virus type 1 (hsv-1) is an e3 ubiquitin ligase of the ring finger class that is required for efficient lytic infection and reactivation from latency. other alphaherpesviruses also express icp0-related ring finger proteins, but these have limited homology outside the core ring domain. existing evidence indicates that icp0 family members have similar properties, but there has been no systematic comparison of the biochemical activities and biological f ... | 2010 | 20106921 |
| characterization of the nuclear import and export mechanisms of bovine herpesvirus-1 infected cell protein 27. | in previous study, we have identified a nuclear localization signal (nls) and a nucleolar localization signal (nols) in bovine herpesvirus-1 (bhv-1) infected cell protein 27 (bicp27), which targets predominantly to the nucleolus. furthermore, the c-terminal 300 amino acid residues targets exclusively to the cytoplasm, suggesting that bicp27 might contain a nuclear export signal (nes). amino acid sequence analysis revealed that there is a cluster of leucine-rich residues resembling a nes. heterok ... | 2010 | 20109505 |
| development and statistical validation of a guinea pig model for vaccine potency testing against infectious bovine rhinothracheitis (ibr) virus. | infectious bovine rhinothracheitis (ibr) caused by bovine herpesvirus 1 (bohv-1) infection is distributed worldwide. bohv-1 either alone or in association with other respiratory cattle pathogens causes significant economic losses to the livestock industry. the aim of this work was to validate a guinea pig model as an alternative method to the current bohv-1 vaccine potency testing in calves. guinea pigs were immunized with two doses of vaccine, 21 days apart and sampled at 30 days post vaccinati ... | 2010 | 20123054 |
| serological evidence of bovine herpesvirus-1 (bohv-1) infection in yaks (peophagus grunniens) from the national research centre on yak, india. | bovine herpesvirus-1 (bohv-1) causes a variety of disease syndromes including, respiratory, nervous and reproductive disorders both in domestic as well as wild bovines and the disease is prevalent throughout the world including india. in this study, serum samples of yaks were screened for serological evidence of bohv-1 in a yak farm with history of abortion and kerato-conjuctivitis by competition-elisa. the result of seroprevalence of bohv-1 infections in yaks (poephagus grunniens) revealed that ... | 2010 | 20143159 |
| immunization of cattle with recombinant newcastle disease virus expressing bovine herpesvirus-1 (bhv-1) glycoprotein d induces mucosal and serum antibody responses and provides partial protection against bhv-1. | bovine herpesvirus-1 (bhv-1) is a major cause of respiratory tract diseases in cattle. vaccination of cattle against bhv-1 is a high priority. a major concern of currently modified live bhv-1 vaccines is their ability to cause latent infection and subsequent reactivation resulting in many outbreaks. thus, there is a need for alternative strategies. we generated two recombinant newcastle disease viruses (ndvs) expressing the glycoprotein d (gd) of bhv-1 from an added gene. one recombinant, rlasot ... | 2010 | 20189484 |
| protein display by bovine herpesvirus type 1 glycoprotein b. | glycoprotein b (gb) of bovine herpesvirus 1 (bhv-1), a major component of the viral envelope, is essential for membrane fusion during entry and cell-to-cell spread. it is cleaved in the trans-golgi network by the proprotein convertase furin. integration of the open reading frame (orf) encoding a mutated gb with a second furin cleavage site and mature boifn-alpha as intervening peptide between the amino-terminal (nh(2)) and carboxy-terminal (cooh) gb subunits yielded recombinant bhv-1/gb2fuifn-al ... | 2010 | 20193990 |
| point mutations in bhv-1 us3 gene abolish its ability to induce cytoskeletal changes in various cell types. | the us3 gene is conserved among alphaherpesviruses and codes for a protein kinase, a multifunctional protein involved in many phases of virus infection, like nuclear egress, modulation of apoptosis and modification of the cellular cytoskeleton. bovine herpesvirus (bhv-1), a member of the alphaherpesvirinae, contains an open reading frame homologous to us3 of other herpesviruses, which has been identified as a serine/threonine kinase (takashima, y., tamura, h., xuan, x., otsuka, h., 1999. identif ... | 2010 | 20197221 |
| should the domestic buffalo (bubalus bubalis) be considered in the epidemiology of bovine herpesvirus 1 infection? | only limited information is available on the epidemiology and pathogenesis of bovine herpesvirus 1 (bohv-1) in domestic buffalos. in this study, a virulent bohv-1 field strain isolated from cattle was inoculated into buffaloes to evaluate their susceptibility to the virus and to investigate the establishment of viral latency through clinical, virological and serological investigations. latency was also studied by attempting viral reactivation using pharmacological induction. six of seven male, 5 ... | 2010 | 20206452 |
| bovine herpesvirus 5 bicp0 complements the bovine herpesvirus 1 homolog. | bovine herpesvirus 1 (bohv-1) and bohv-5 are closely related (82% amino acid identity) but differ strongly in neuropathogenesis. the immediate-early gene for bicp0 is less conserved (70% amino acid identity) and may contribute to a dissimilar phenotype. a peculiar difference is a guanosine hexamer in the bicp0-1 gene which aligns with only five guanosines in the bicp0-5 gene and therefore results in a frameshift in the latter open reading frame. thus, the c-terminal amino acid sequence (residues ... | 2010 | 20211531 |
| glycoprotein d of bovine herpesvirus 5 (bohv-5) confers an extended host range to bohv-1 but does not contribute to invasion of the brain. | bovine herpesvirus 1 (bohv-1) and bohv-5 are closely related pathogens of cattle, but only bohv-5 is considered a neuropathogen. we engineered intertypic gd exchange mutants with bohv-1 and bohv-5 backbones in order to address their in vitro and in vivo host ranges, with particular interest in invasion of the brain. the new viruses replicated in cell culture with similar dynamics and to titers comparable to those of their wild-type parents. however, gd of bohv-5 (gd5) was able to interact with a ... | 2010 | 20219909 |
| an indirect elisa for serodiagnosis of cattle footrot caused by fusobacterium necrophorum. | a serodiagnostic elisa (rl-elisa) using recombinant truncated leukotoxin protein pl2 (aa 311-644) of fusobacterium necrophorum as antigen was developed for detection of antibodies against f. necrophorum from cattle footrot. in rl-elisa, the recombinant diagnostic antigen showed no cross-reaction with antisera against bovine foot and mouth disease virus, bovine rhinotracheitis virus, bovine viral diarrhea virus, bovine rotavirus type a, bovine escherichia coli, and bovine salmonella. the rl-elisa ... | 2010 | 20304080 |
| effect of foot-and-mouth disease virus capsid precursor protein and 3c protease expression on bovine herpesvirus 1 replication. | several reports have previously shown that expression of the foot-and-mouth disease virus (fmdv) capsid precursor protein encoding region p1-2a together with the 3c protease (p1-2a/3c) results in correct processing of the capsid precursor into vp0, vp1 and vp3 and formation of fmdv capsid structures that are able to induce a protective immune response against fmdv challenge after immunization using naked dna constructs or recombinant viruses. to elucidate whether bovine herpesvirus 1 (bhv-1) mig ... | 2010 | 20333533 |
| bighorn sheep fetal lung cell line for detection of respiratory viruses. | pneumonia is an important disease of bighorn sheep (bhs) that is primarily responsible for the drastic decline in numbers of these animals in north america. members of the genus mannheimia and pasteurella have frequently been isolated from the pneumonic lungs of bhs. antibodies to several respiratory viruses, including bovine parainfluenza virus 3 (bpiv-3), bovine respiratory syncytial virus (brsv), bovine viral diarrhea virus (bvdv), and bovine herpesvirus 1 (bohv-1), have been detected in herd ... | 2010 | 20357964 |
| immunogenicity of a modified-live virus vaccine against bovine viral diarrhea virus types 1 and 2, infectious bovine rhinotracheitis virus, bovine parainfluenza-3 virus, and bovine respiratory syncytial virus when administered intranasally in young calves. | the immunogenicity of an intranasally-administered modified-live virus (mlv) vaccine in 3-8 day old calves was evaluated against bovine viral diarrhea virus (bvdv) types 1 and 2, infectious bovine rhinotracheitis (ibr) virus, parainfluenza-3 (pi-3) virus and bovine respiratory syncytial virus (brsv). calves were intranasally vaccinated with a single dose of a multivalent mlv vaccine and were challenged with one of the respective viruses three to four weeks post-vaccination in five separate studi ... | 2010 | 20381643 |
| erratum to: serological evidence of bovine herpesvirus-1 (bohv-1) infection in yaks (peophagus grunniens) from the national research centre on yak, india. | | 2010 | 20390352 |
| productive infection and bicp0 early promoter activity of bovine herpesvirus 1 are stimulated by e2f1. | bovine herpesvirus 1 (bohv-1) is an important viral pathogen of cattle. like other members of the subfamily alphaherpesvirinae, bohv-1 establishes latency in sensory neurons and has the potential to reactivate from latency. dexamethasone (dex) treatment of latently infected calves or rabbits consistently leads to reactivation from latency. the bohv-1 transcript encoding the infected cell protein 0 (bicp0) is consistently detected during reactivation from latency, in part because the bicp0 early ... | 2010 | 20410283 |
| small noncoding rnas encoded within the bovine herpesvirus 1 latency-related gene can reduce steady-state levels of infected cell protein 0 (bicp0). | following acute infection in mucosal epithelium, bovine herpes virus 1 (bhv-1) establishes lifelong latency in sensory neurons within trigeminal ganglia. the latency-related rna (lr-rna) is abundantly expressed in sensory neurons of latently infected calves. expression of lr proteins is necessary for the latency reactivation cycle because a mutant virus that does not express lr proteins is unable to reactivate from latency after dexamethasone treatment. lr-rna sequences also inhibit bicp0 expres ... | 2010 | 20410286 |
| a serological survey of antibodies against bovine herpesvirus-1 in yak (poephagus grunniens) in arunachal pradesh in india. | serum samples were collected from 254 yak (poephagus grunniens, presently bos grunniens) in different yak tracts of india. these samples were then screened by virus neutralisation test (vnt) and avidin-biotin enzyme-linked immunosorbent assay (ab-elisa) to study the seroprevalence of antibodies against bovine herpesvirus type 1 (bhv-1). the overall seroprevalence in yak was found to be 41% (105) by vnt and ab-elisa. the sex of the animal, whether it was on a farm or free-ranging and the location ... | 2009 | 20462162 |
| effect of dietary boron on physiological responses in growing steers inoculated with bovine herpesvirus type-1. | thirty-six angus and angus×simmental steers were fed one of three dietary treatments; (1) control (no supplemental b), (2) 5 mg supplemental b/kg, and (3) 15 mg supplemental b/kg for 47 days to determine the effects of dietary boron (b) on disease resistance following an inoculation with bovine herpesvirus type-1 (bhv-1). on day 34 of the study steers were inoculated intranasally with bhv-1. rectal temperatures began to elevate at day 2, and plasma tumor necrosis factor-α concentrations increase ... | 2011 | 20493506 |
| 2,3,7,8-tetrachlorodibenzo-p-dioxin modifies expression and nuclear/cytosolic localization of bovine herpesvirus 1 immediate-early protein (bicp0) during infection. | our previous studies have demonstrated that 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) increases bovine herpesvirus 1 (bhv-1) replication through a dose-dependent increase in cytopathy and increased viral titer. furthermore, tcdd was able to trigger bhv-1-induced apoptosis by up-regulating the activation of initiator caspases 8 and 9, as well as of effector caspase 3. since tcdd activates caspase 3 after 4 h of infection, we have hypothesized an involvement of bhv-1 infected cell protein 0 (bicp ... | 2010 | 20506271 |
| recombinant n-terminal part of bovine herpesvirus 1 icp27 protein: its preparation, purification, and use for raising specific antiserum. | recombinant n-terminal part of bovine herpesvirus-1 (bohv-1) icp27 protein fused with thioredoxin and his-tag ("the recombinant protein") expressed in escherichia coli was purified by the ni2+-nta affinity chromatography and used for the preparation of antiserum by immunization of rabbits. the antiserum recognized the recombinant protein in western blot analysis and was able to detect bohv-1 icp27 in the nucleoli of bohv-1-infected mdbk cells. these results showed that such an antiserum could se ... | 2010 | 20545445 |
| seroepidemiology of bovine herpesvirus 1 (bhv1) infection among estonian dairy herds and risk factors for the spread within herds. | the objectives of this study were to reassess the herd level and within-herd prevalence of bovine herpesvirus 1 (bhv1) infection in estonian dairy cattle, estimate the sensitivity and specificity of the enzyme-linked immunosorbent assay (elisa) for bulk tank milk (btm) testing and determine the risk factors related to high prevalence of the infection in herds. to estimate the herd prevalence, btm samples from each of the 1,205 herds that sell milk to dairy companies were analysed for bhv1 antibo ... | 2010 | 20598386 |
| immunization and challenge experiments with a new modified live bovine herpesvirus type 1 marker vaccine prototype adjuvanted with a co-polymer. | western european control programs against bovine herpesvirus type 1 (bohv-1) infections utilize attenuated bohv-1 marker vaccines with a deletion of the glycoprotein e (ge) encoding gene. however, a recent study demonstrated the potential risk of virulence recovery of ge-deleted bohv-1 marker vaccine strains due to recombination (muylkens et al. [15]). based on an infectious bacterial artificial chromosome clone, a ge- and thymidine kinase (tk)-gene-deleted bohv-1 mutant (bohv-1deltagedeltatk) w ... | 2010 | 20600486 |
| a comparative analysis of envelope and tegument proteins of suid herpesvirus 1, bovine herpesvirus 1 and bovine herpesvirus 5. | the aim of this study was to analyze the disordered regions (drs) in the envelope and tegument proteins of three closely related herpesviruses: bovine herpesvirus 1, bovine herpesvirus 5 and suid herpesvirus 1. tegument proteins showed a greater percentage of drs than the envelope proteins did. regions of disorder were found in the less conserved portions of the proteins, n-terminal and c-terminal regions, and, in a few cases, functionally important sites. the presence of drs is an important fac ... | 2010 | 20607317 |
| bovine herpesvirus type 1 (bhv-1) is an important cofactor in the bovine respiratory disease complex. | bhv-1 is an important pathogen of cattle. because of its ability to induce immune suppression, bhv-1 is an important agent in the multifactorial disorder, bovine respiratory disease complex (brdc). bhv-1 encodes several proteins that inhibit various arms of the immune system suggesting that these proteins are important in the development of brdc. | 2010 | 20619186 |
| validation of an indirect elisa to detect antibodies against bohv-1 in bovine and guinea-pig serum samples using iso/iec 17025 standards. | two elisas to quantify antibodies to bohv-1 in the sera of cattle and immunized guinea pigs were developed and validated using iso/iec 17025 standards. the cut-off value of the assay was established at 20% positivity of a high positive control for screening of cattle. using this threshold, the assay properly classified the oie bovine reference sera eu1, eu2 and eu3. for vaccine potency testing, a cut-off of 40% was selected for both species. the reliability of the assays, given by their diagnost ... | 2010 | 20655331 |
| antibodies to pathogenic livestock viruses in a wild vicuña (vicugna vicugna) population in the argentinean andean altiplano. | serum samples from 128 wild vicuñas (vicugna vicugna) were tested for antibodies (ab) to rotavirus (rv), bovine parainfluenza virus 3 (bpiv-3), bovine herpesvirus-1 (bhv-1), bovine viral diarrhea virus (bvdv-1), foot-and-mouth disease virus (fmdv), bluetongue virus (btv), equine herpesvirus-1 (ehv-1), and influenza a virus equine (eiv). samples were collected in cieneguillas province of jujuy, in northern argentina. feces from 44 vicuñas were also collected to investigate rv shedding. llamas (la ... | 2010 | 20688660 |
| abortifacient vaccines and bovine herpesvirus-1. | | 2010 | 20712064 |
| microbiological and pathological examination of fatal calf pneumonia cases induced by bacterial and viral respiratory pathogens. | the infectious origin of fatal cases of calf pneumonia was studied in 48 calves from 27 different herds on postmortem examination. lung tissue samples were examined by pathological, histological, bacterial culture, virus isolation and immunohistochemical methods for the detection of viral and bacterial infections. pneumonia was diagnosed in 47/48 cases and infectious agents were found in 40/47 (85%) of those cases. the presence of multiple respiratory pathogens in 23/40 (57.5%) cases indicated t ... | 2010 | 20713325 |
| water-extracted polysaccharides from azadirachta indica leaves: structural features, chemical modification and anti-bovine herpesvirus type 1 (bohv-1) activity. | in this study, we have analyzed the pectic arabinogalactan isolated from azadirachta indica and its chemically sulfated derivative. the native polysaccharide had an apparent molecular mass of 80 kda and is made up of (1→5)-/(1→3,5)-linked α-l-arabinosyl, (1→3)-/(1→6)-/(1→3,6)-linked β-d-galactosyl, and terminal-rhamnosyl and α-l-arabinosyl residues. these macromolecules showed activity against bovine herpesvirus type-1. their inhibitory concentration 50% values ranging from 31.12 to 105.25 μg/ml ... | 2010 | 20732349 |
| chitosan based delivery systems for mucosal immunization against bovine herpesvirus 1 (bhv-1). | bovine herpesvirus 1 (bhv-1), is a major pathogen of cattle which causes serious infections, including infectious bovine rhinotracheitis (ibr)/infectious pustular vulvovaginitis (ipv). at present, bhv-1 is still a serious threat to animal health and productivity in turkey, hence to develop a more efficient and economical vaccine system against bhv-1 is certainly an important necessity. a mucosal vaccination strategy would provide both mucosal and systemic immune responses to protect disease prog ... | 2010 | 20800678 |
| in vitro antiviral activity from acanthospermum australe on herpesvirus and poliovirus. | context: the asteraceae family has been of interest to researchers due to the presence of polyphenolic compounds, mainly flavonoids, which demonstrated antiviral activity. objective: the hydroethanol extract of the aerial parts of acanthospermum australe (loefl.) kuntze (asteraceae) and its fractions, were evaluated in vitro for their potential cytotoxic and antiviral activity against bovine herpesvirus and human poliovirus. materials and methods: the sulforhodamine b colorimetric assay were use ... | 2011 | 20819023 |
| the nucleolus and viral infection. | the nucleolus is a subnuclear structure of eukaryocytes. it was thought that nucleolus only participates in the biogenesis and processing of rrna. however, more and more evidence shows that it has many other functions, such as trna precursor processing, stress sensing and it is also involved in gene silencing, senescence and cell cycle regulation. here, we summarize the recent understandings about the nucleolar functions, the regulation of nucleolar localization of proteins and the role that the ... | 2010 | 20960288 |
| herpes virus reactivation by low-intensity diode and co₂ lasers. | the herpes virus enters into latency after symptomatic or asymptomatic herpetic infection. during latency, the virus has no impact on infected cells. however, internal or external stimuli, including certain lasers, can induce virus reactivation. | 2010 | 20969439 |
| cd8+ t cell responses against tap-inhibited cells are readily detected in the human population. | target cell recognition by ctls depends on the presentation of peptides by hla class i molecules. tumors and herpes viruses have adopted strategies to greatly hamper this peptide presentation at the important bottleneck, the peptide transporter tap. previously, we described the existence of a cd8(+) ctl subpopulation that selectively recognizes such tap-deficient cells in mouse models. in this study, we show that the human counterpart of this ctl subset is readily detectable in healthy subjects. ... | 2010 | 20980626 |
| study of the kinetics of antibodies titres against viral pathogens and detection of rotavirus and parainfluenza 3 infections in captive crias of guanacos (lama guanicoe). | a longitudinal study was conducted to investigate the presence of antibodies (ab) to rotavirus (rv), parainfluenza-3 virus (pi-3), bovine herpesvirus-1 (bohv-1), bovine viral diarrhoea virus (bvdv-1) and bluetongue virus (btv) in eleven guanaco's crias (chulengos) relocated from rio negro to buenos aires province (argentina) and reared in captivity for a year in an experimental field. serum samples were collected periodically to detect the evidence of viral infections. faecal samples were collec ... | 2010 | 21062425 |
| a bovine herpesvirus type 1 mutant virus with truncated glycoprotein e cytoplasmic tail has defective anterograde neuronal transport in rabbit dorsal root ganglia primary neuronal cultures in a microfluidic chamber system. | bovine herpesvirus type 1 (bhv-1) is an important component of the bovine respiratory disease complex (brdc) in cattle. following primary intranasal and ocular infection of cattle, bhv-1 establishes lifelong latent infection in trigeminal ganglia (tg). upon reactivation from latency, the virus is transported from neuronal cell bodies in the tg to projected nerve endings in nose and cornea of latently infected cattle where the virus shedding occurs. this property of bhv-1 plays a significant role ... | 2010 | 21080783 |
| [infectious bovine rhinotracheitis (ibr) in the canton of jura: an epidemiological outbreak investigation]. | following an abortion in a beef herd in the summer of 2009, three outbreaks of infectious bovine rhinotracheitis (ibr) were diagnosed in the cantons of jura and neuchatel. an epidemiological outbreak investigation was conducted with the aims to identify the source of introduction of the bovine herpes virus 1 (bohv-1) into the affected herds and to prevent further spread of the disease. the attack rates in the three outbreak farms were 0.89, 0.28 and 0, respectively. bohv-1 could be isolated from ... | 2010 | 21104629 |
| swine adipose stromal cells loaded with recombinant bovine herpesvirus 4 virions expressing a foreign antigen induce potent humoral immune responses in pigs. | increasingly effective vaccination strategies are needed to counteract the high incidence of contagious diseases associated with intensive swine breeding. recombinant viral vaccines are a promising new avenue in this direction. key features of viral vectors suitable for immunoprophylaxis are safety, ease of manipulation and the ability to replicate in a variety of hosts. most of the above requirements are met by bovine herpesvirus 4 (bohv-4), a non-pathogenic dsdna virus capable of infecting a b ... | 2010 | 21115049 |
| quebec: detection of bovine lymphotropic herpesvirus dna in tissues of a bovine aborted fetus. | | 2010 | 21119872 |
| multiplex real-time rt-pcr detection of three viruses associated with the bovine respiratory disease complex. | bovine respiratory disease complex (brdc) is considered the most significant illness associated with feedlot cattle in north america and possibly worldwide. brdc is a multi-factorial disease with environmental conditions interacting with multiple viral and bacterial pathogens to produce severe respiratory illness. bovine herpesvirus 1, bovine viral diarrhoea virus and bovine parainfluenza virus 3 are three of the major viruses associated with brdc. in this study, a multiplex real-time rt-pcr usi ... | 2010 | 21126537 |
| seroprevalence of infectious bovine rhinotracheitis and bovine viral diarrhea virus type 1 and type 2 in non-vaccinated cattle herds in the pacific region of central costa rica. | the objectives of this cross-sectional study were to estimate the seroprevalence of infectious bovine rhinotracheitis (ibr, bhv-1) and bovine viral diarrhea virus (bvdv) in a population of non-vaccinated, double purpose, dairy and beef herds in the pacific region of central costa rica. blood samples were collected from a total of 496 animals from 35 herds. sera were tested for antibodies against bhv-1(ibr) and bvdv types 1 and 2 using serum neutralization test. the average number of animals test ... | 2010 | 21128116 |
| withdrawn: corrigendum to "characterization of the nuclear import and export mechanisms of bovine herpesvirus-1 infected cell protein 27" [virus research 149 (2010) 95-103]. | this article has been withdrawn at the request of the authors. the publisher apologizes for any inconvenience this may cause. the full elsevier policy on article withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. | 2010 | 21130511 |
| withdrawn: corrigendum to "characterization of the nuclear and nucleolar localization signals of bovine herpesvirus-1 infected cell protein 27" [virus res. 145 (2009) 312-320]. | this article has been withdrawn at the request of the authors. the publisher apologizes for any inconvenience this may cause. the full elsevier policy on article withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. | 2010 | 21134698 |
| [testing for btv, bvdv and bhv-1 in blood samples of new world camelids kept in middle germany]. | the susceptibility of camelids for infectious agents which may result in severe economic losses or which are strictly regulated for epidemiological reasons in farm animals potentially causes a mutual risk of transmission. this study aimed to investigate the presence of antibodies against bovine herpesvirus 1 (bhv-1), bluetongue virus (btv) and bovine viral diarrhoea virus (bvdv) as well as the presence of pestivirus antigen in new world camelids in central germany. therefore 107 serum samples fr ... | 2010 | 21141278 |
| serological evidences of bovine herpesvirus-1 infection in bovines of organized farms in india. | bovine herpesvirus type-1 (bhv-1) is an important pathogen of cattle causing a variety of clinical signs, including the upper respiratory tract infection, infectious bovine rhinotracheitis (ibr). infectious bovine rhinotracheitis, a highly infectious disease of cattle and buffaloes, occurs throughout the world including india. the present study based on micro-serum neutralization test reports the sero-epidemiology of bhv-1 infection in cattle and buffaloes from different parts of india. serum sa ... | 2010 | 21156033 |
| the capacity of ul49.5 proteins to inhibit tap is widely distributed among members of the genus varicellovirus. | the lifelong infection by varicelloviruses is characterized by a fine balance between the host immune response and immune evasion strategies used by these viruses. virus-derived peptides are presented to cytotoxic t lymphocytes by major histocompatibility complex (mhc) class i molecules. the transporter associated with antigen processing (tap) transports the peptides from the cytosol into the endoplasmic reticulum, where the loading of mhc-i molecules occurs. the varicelloviruses bovine herpesvi ... | 2010 | 21159875 |
| a protein (orf2) encoded by the latency-related gene of bovine herpesvirus 1 interacts with notch1 and notch3. | like other alphaherpesvirinae subfamily members, bovine herpesvirus 1 (bhv-1) establishes latency in sensory neurons. the latency-related rna (lr-rna) is abundantly expressed in latently infected sensory neurons. an lr mutant virus with stop codons at the amino terminus of the first open reading frame (orf) in the lr gene (orf2) does not reactivate from latency, in part because it induces higher levels of apoptosis in infected neurons. orf2 is not the only viral product expressed during latency, ... | 2010 | 21191019 |
| regulation of promyelocytic leukemia (pml) protein levels and cell morphology by bovine herpesvirus 1 infected cell protein 0 (bicp0) and mutant bicp0 proteins that do not localize to the nucleus. | bhv-1 is an important pathogen of cattle. the infected cell protein 0 (bicp0) encoded by bhv-1 is an important regulatory protein because it is constitutively expressed and can activate all viral promoters. the mechanism by which bicp0 activates viral promoters is not well understood because bicp0 does not appear to be a sequence specific binding protein. a c(3)hc(4) zinc ring (really interesting novel gene) motif at the n-terminus of bicp0 has e3 ubiquitin ligase activity, which is important fo ... | 2011 | 21215282 |
| detection of bovine herpesvirus 1 and 5 in semen from brazilian bulls. | bovine herpesvirus 1 (bohv-1) and 5 (bohv-5) are important pathogens of the respiratory and genital tract of cattle and may also affect the central nervous system and cause meningoencephalitis. both virus types are estimated to be widely distributed in southern brazil. in the present study, bohv-1 and/or bohv-5 dna were detected in bovine semen samples from two states of brazil by two species-specific nested polymerase chain reactions (npcrs). these npcrs were used to assay 53 samples of fresh s ... | 2011 | 21247624 |
| prevalence of pasteurella multocida and other respiratory pathogens in the nasal tract of scottish calves. | the prevalence of pasteurella multocida, a cause of bovine respiratory disease, was studied in a random sample of beef suckler and dairy farms throughout scotland, by means of a cross-sectional survey. a total of 637 calves from 68 farms from six geographical regions of scotland were sampled between february and june 2008. deep nasal swabs were taken, and samples that were culture-positive for p multocida were confirmed by pcr. prevalence of p multocida was 17 per cent (105 of 616 calves); 47 pe ... | 2010 | 21257416 |
| indications of a relationship between buying-in policy and infectious diseases on dairy farms in wales. | during the period february to may 2008, bulk milk samples were collected from 57 dairy farms throughout wales in the framework of a voluntary somatic cell count project. bulk milk samples were tested for antibodies to bovine viral diarrhoea virus (bvdv), bovine herpesvirus type 1 (bhv-1) and leptospira hardjo, and samples were also tested for the presence of bvdv antigen by pcr. a questionnaire was used to determine whether the herd was open or closed, what the vaccination status was, and to obt ... | 2010 | 21257463 |
| development and evaluation of a luminex multiplex serology assay to detect antibodies to bovine herpes virus 1, parainfluenza 3 virus, bovine viral diarrhoea virus, and bovine respiratory syncytial virus, with comparison to existing elisa detection methods. | detection of circulating antibodies to bovine herpes virus 1 (bhv-1), parainfluenza 3 virus (pi3v), bovine viral diarrhoea virus (bvdv) and bovine respiratory syncytial virus (brsv) using elisa is widely used for veterinary diagnostics and surveillance. in this paper, the potential of a multiplex serology test based on luminex technology, where all antibodies are simultaneously detected in a single assay was investigated. the performance of "in-house" separate elisas which use relatively crude l ... | 2011 | 21277304 |
| inhibition of mouse tap by immune evasion molecules encoded by non-murine herpesviruses. | herpesviruses escape elimination by cytotoxic t lymphocytes through specific interference with the antigen-presenting function of mhc class i (mhc i) molecules. the transporter associated with antigen processing (tap) forms a bottleneck in the mhc i antigen presentation pathway. the fact that multiple viruses, especially herpesviruses, encode molecules blocking tap function is a case in point. the action of these viral immuno evasins is usually potent and very specific, making these proteins val ... | 2011 | 21292324 |
| detection of bovine herpesvirus 1 sequences in yaks (bos grunniens) with keratoconjunctivitis, using a highly sensitive nested polymerase chain reaction. | thirty-seven yaks (bos grunniens) with keratoconjunctivitis and 22 healthy yaks were used to investigate the role of bovine herpesvirus 1 (bohv-1) in keratoconjunctivitis in yaks. nucleic acid sequences of bohv-1 glycoproteins b and e were detected in conjunctival swabs from all yaks with keratoconjunctivitis using a nested polymerase chain reaction (pcr). in 21 yaks, bohv-1 sequences were detected along with moraxella bovis (m. bovis) and neisseria spp. the amplified bohv-1 sequences were ident ... | 2010 | 21309468 |
| infection of cultured bovine cells with bovine herpesvirus 1 (bhv-1) or sendai virus induces different beta interferon subtypes. | in contrast to mice or humans, cattle contain three beta interferon (ifn-ß) genes with distinct transcriptional promoters suggesting ifn-ß gene expression is not stimulated the same by different viruses. to test this hypothesis, we compared expression of the three ifn-ß subtypes after infection with a rna virus, sendai, versus a large dna virus, bovine herpesvirus 1 (bhv-1). infection of low passage bovine kidney (bk) or established bovine kidney cells (crib) with sendai virus has consistently l ... | 2011 | 21316405 |
| comparative analysis of replication characteristics of bohv-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment. | abstract: in general, members of the alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. bovine herpesvirus type 1 (bohv-1) may replicate at both sites and cause two major clinical entities designated as infectious bovine rhinotracheitis (ibr) and infectious pustular vulvovaginitis/balanoposthitis (ipv/ipb) in cattle. it has been hypothesized that subtype 1.1 invades preferentially the upper respiratory mucosa wherea ... | 2011 | 21324115 |
| induction of specific cytotoxic activity for bovine herpesvirus-1 by dna immunization with different adjuvants. | it is well documented that adjuvants improve the immune response generated by traditional viral vaccines; however, less is known about their effects on the immune response elicited by dna vaccines. in this study, we have investigated the use of adjuvants, and have analyzed the humoral and cellular specific immune responses elicited by dna vaccines based on the bohv-1 glycoprotein d (secreted version) in pcineo vector with and without montanide isa25 (o/w), isa206 vg (seppic) and cliptox™ (natura ... | 2011 | 21443903 |
| development and validation of a triplex real-time pcr assay for the rapid detection and differentiation of wild-type and glycoprotein e-deleted vaccine strains of bovine herpesvirus type 1. | two important components of control programs for bovine herpesvirus type 1 (bohv-1), a major pathogen of cattle, are the detection of outbreaks and vaccination with glycoprotein e (ge)-deleted marker vaccines. in addition to serology, rapid and accurate investigation of bohv-1 and genetic differentiation of vaccine and wild-type strains are also important methods. therefore, a triplex quantitative real-time polymerase chain reaction (qpcr) for testing bohv-1 was developed. apart from a bohv-1 sp ... | 2011 | 21458493 |
| biphasic activation of pi3k/akt and mapk/erk1/2 signaling pathways in bovine herpesvirus type 1 infection of mdbk cells. | abstract: many viruses have been known to control key cellular signaling pathways to facilitate the virus infection. the possible involvement of signaling pathways in bovine herpesvirus type 1 (bohv-1) infection is unknown. this study indicated that infection of mdbk cells with bohv-1 induced an early-stage transient and a late-stage sustained activation of both phosphatidylinositol 3-kinase (pi3k)/akt and mitogen activated protein kinases/extracellular signal-regulated kinase 1/2 (mapk/erk1/2) ... | 2011 | 21492439 |
| seroprevalence and risk factors associated to bovine herpesvirus 1 (bhv-1) infection in non-vaccinated dairy and dual purpose cattle herds in ecuador. | a cross-sectional study was carried out to determine the seroprevalence and risk factors associated to bovine herpesvirus 1 (bhv-1) infection in non-vaccinated dairy and dual purpose cattle herds from ecuador. a total of 2367 serum samples from 346 herds were collected from june 2008 through february 2009. a questionnaire, which included variables related to cattle, health, management measures and environment was filled out in each herd. a commercial indirect elisa test was used to determine the ... | 2011 | 21501883 |
| detection and differentiation of bovine herpesvirus 1 and 5 using a multiplex real-time polymerase chain reaction. | a multiplex real-time pcr was developed for the detection and differentiation of two closely related bovine herpesviruses 1 (bohv-1) and 5 (bohv-5). the multiplex real-time pcr combines a duplex real-time pcr that targets the dna polymerase gene of bohv-1 and bohv-5 and a real-time pcr targeting mitochondrial dna, as a house-keeping gene, described previously by cawthraw et al. (2009). the assay correctly identified 22 bohv-1 and six bohv-5 isolates from the biosecurity sciences laboratory virus ... | 2011 | 21540057 |
| in vitro-generated interspecific recombinants between bovine herpesviruses 1 and 5 show attenuated replication characteristics and establish latency in the natural host. | abstract: background: interspecific recombinant viruses r1deltagc and r2deltagi were isolated after in vitro co-infection with bohv-1 and bohv-5, two closely related alphaherpesviruses that infect cattle. the genetic characterization of r1deltagc and r2deltagi showed that they are composed of different sections of the parental genomes. the aim of this study was the characterization of the in vivo behavior of these recombinants in the natural host. results: four groups of four 3-month-old calves ... | 2011 | 21592326 |
| protection from persistent infection with a bovine viral diarrhea virus (bvdv) type 1b strain by a modified-live vaccine containing bvdv types 1a and 2, infectious bovine rhinotracheitis virus, parainfluenza 3 virus and bovine respiratory syncytial virus. | recent studies showed that bvdv-1b subgenotype is dominant in north and south american field bvdv isolates. however, nearly all commercially available bvdv-1 vaccines contain bvdv-1a strains. in order to study the efficacy of bvdv-1a vaccine against bvdv-1b infection, this study was designed to evaluate a modified-live vaccine (mlv) containing bvdv-1a and bvdv-2 strains for its efficacy in prevention of persistent infection of fetuses against bvdv-1b strain, when the heifers were vaccinated prio ... | 2011 | 21596076 |
| analysis of the cell cycle regulatory protein (e2f1) after infection of cultured cells with bovine herpesvirus 1 (bhv-1) or herpes simplex virus type 1 (hsv-1). | the e2f family of cellular transcription factors controls cell cycle progression and cell death. during cell cycle progression, activated cyclin-dependent kinases phosphorylate the retinoblastoma (rb) protein, causing the release and activation of e2f family members. previous studies demonstrated that bovine herpes virus 1 (bhv-1) productive infection increases e2f1 protein levels, the bicp0 early promoter is activated more than 100 fold by e2f1 or e2f2, and silencing e2f1 reduced the efficiency ... | 2011 | 21624405 |
| the bovine herpesvirus type 1 envelope protein us9 acidic domain is crucial for anterograde axonal transport. | in this study, we examined the functional role of bovine herpesvirus type 1 (bhv-1) us9 acidic domain residues 83-90 in the anterograde axonal transport of the virus in calves (natural host), rabbits, and in cultured neurons. a mutant virus strain lacking us9 residues 83-90 (bhv-1 us9 δ83-90) and the rescued virus (bhv-1 us9 r83-90) replicated efficiently in the nasal and ocular epithelium during primary infection and established latency in the trigeminal ganglia (tg). however, upon reactivation ... | 2011 | 21640524 |
| preliminary association analysis of tlr9 gene polymorphisms and immune parameters in an italian holstein calves population. | this preliminary study was aimed at evaluating the association between single nucleotide polymorphisms (snps) on toll like receptor 9 (tlr9) gene and some immunological parameters in a population of italian holstein calves. | 2011 | 21645300 |
| comparative study on the in vitro and in vivo properties of two bovine herpesvirus-5 reference strains. | bovine herpesvirus 5 (bohv-5) is an alphaherpesvirus responsible for meningoencephalitis in young cattle and it is antigenically and genetically related to bovine herpesvirus 1. bohv-5 outbreaks are sporadic and restricted in their geographical distribution, being mostly detected in the southern hemisphere. the n569 and a663 strains are prototypes of the "a" and "b" subtypes of bohv-5, however, scarce information about their in vitro and in vivo properties is currently available. | 2011 | 21651813 |
| development of a sandwich elisa for the detection of bovine herpesvirus type 1. | to develop a standard enzyme-linked immunosorbent assay (elisa) for the detection of bovine herpesvirus type 1 (bhv-1). | 2011 | 21771677 |