| effects of aureomycin on listeria monocytogenes and the production of encephalitic symptoms in rabbits. | | 1952 | 24542095 |
| pathological findings in the red fox (vulpes vulpes), stone marten (martes foina) and raccoon dog (nyctereutes procyonoides), with special emphasis on infectious and zoonotic agents in northern germany. | anthropogenic landscape changes contributed to the reduction of availability of habitats to wild animals. hence, the presence of wild terrestrial carnivores in urban and peri-urban sites has increased considerably over the years implying an increased risk of interspecies spillover of infectious diseases and the transmission of zoonoses. the present study provides a detailed characterisation of the health status of the red fox (vulpes vulpes), stone marten (martes foina) and raccoon dog (nyctereu ... | 2017 | 28399176 |
| pregnancy-related listeriosis. | about one in seven cases of listeriosis occurs in pregnant women, and, although listeriosis is rare, it is the third leading cause of death from food-borne infections. pregnancy-related listeriosis increases the risk for fetal and neonatal mortality by approximately 21%. during pregnancy, infections are more likely to occur in the third trimester (66%) than the first trimester (3%). however, fetal and neonatal adverse effects are less common as gestational age increases or with older gestational ... | 2017 | 28398675 |
| impact of acanthamoeba cysts on stress resistance of salmonella enterica serovar typhimurium, yersinia enterocolitica 4/o:3, listeria monocytogenes 1/2a, and escherichia coli o:26. | the formation of robust resting cysts enables acanthamoeba to resist harsh environmental conditions. this study investigated to what extent these cysts are resistant to physical and chemical stresses as applied in food industry cleaning and disinfection procedures. moreover, it was assessed whether certain intracystic meat-borne bacterial pathogens are more stress resistant than free-living bacterial monocultures and if intracystic passage and subsequent association with trophozoites induces cro ... | 2017 | 28526786 |
| uv-curable contact active benzophenone terminated quaternary ammonium antimicrobials for applications in polymer plastics and related devices. | a series of uv active benzophenone ([c6h5coc6h4-o-(ch2)n-n+me2r][x-], 4: r = c12h25, n = 3, x- = br- , 5a-c: r = c18h37, n = 3, x- = cl-, br-, i-; 6a-c: r = c18h37, n = 4, x- = cl-, br-, i- ; 7a-c: r = c18h37, n = 6, x- = cl-, br-, i-) terminated c12 and c18 quaternary ammonium salts (qacs) were prepared by thermal or microwave driven menshutkin protocols of the appropriate benzophenone alkyl halide (1a-c, 2a-c, 3a-c) with the corresponding dodecyl- or octadecyl n,n-dimethylamine. all new compou ... | 2017 | 28777541 |
| diversity of endophytic bacteria in a fern species dryopteris uniformis (makino) makino and evaluation of their antibacterial potential against five foodborne pathogenic bacteria. | the fern plant dryopteris uniformis has traditionally been used in herbal medicine and possesses many biological activities. this study was conducted to explore the endophytic bacterial diversity associated with d. uniformis and evaluate their antibacterial potential against foodborne pathogenic bacteria (fpb). among 51 isolated endophytic bacteria (eb), 26 eb were selected based on their morphological characteristics and identified by 16s rrna gene analysis. the distribution of eb was diverse i ... | 2017 | 28418717 |
| molecular characterization of gluten hydrolysing bacillus sp. and their efficacy and biotherapeutic potential as probiotics using caco-2 cell line. | to isolate and characterize indigenous gluten hydrolysing bacteria from wheat sourdough and curd samples and further evaluation of their probiotic potentiality. | 2017 | 28637081 |
| chemical constituents of helichrysum italicum (roth) g. don essential oil and their antimicrobial activity against gram-positive and gram-negative bacteria, filamentous fungi and candida albicans. | the aerial parts of helichrysum italicum (roth) g. don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. the essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). the most o ... | 2017 | 28725151 |
| development and characterization of bioactive edible films from spider crab (maja crispata) chitosan incorporated with spirulina extract. | active food packaging films based on crab chitosan and spirulina extract (se) were developed. the effects of the se incorporation at different levels on physical (color, opacity water vapor and oxygen permeability) and mechanical (tensile strength and elongation at break) properties of chitosan films were investigated. ftir was carried out to observe the potential modifications of the chitosan films when incorporated with se. the obtained results suggested that incorporation of se into chitosan ... | 2017 | 28705500 |
| identification and growth optimization of a marine bacillus dk1-sa11 having potential of producing broad spectrum antimicrobial compounds. | control of harmful bacteria in food, aquaculture, pharmaceuticals, agriculture, hospitals and recreation water pools are of great global concern. marine bacteria are an enormous source of bio-controlling agents. the aim of this study was to identify and optimize the growth conditions including effect of different biotic and abiotic factors on antimicrobial activity of strain dk1-sa11 isolated from qingdao bay of china yellow sea. microscopic characterization, api® 20e and 50 chb kit base carbohy ... | 2017 | 28653930 |
| yvck, a protein required for cell wall integrity and optimal carbon source utilization, binds uridine diphosphate-sugars. | in bacillus subtilis, listeria monocytogenes and in two mycobacteria, it was previously shown that yvck is a gene required for normal cell shape, for optimal carbon source utilization and for virulence of pathogenic bacteria. here we report that the b. subtilis protein yvck binds to uridine diphosphate-sugars like uridine diphosphate-glucose (udp-glc) and uridine diphosphate-n-acetylglucosamine (udp-glcnac) in vitro. using the crystal structure of bacillus halodurans yvck, we identified residues ... | 2017 | 28646159 |
| antimicrobial effect of okcel® h-d prepared from oxidized cellulose. | the antimicrobial effect of okcel® h-d, a topical, absorbable hemostatic textile prepared from oxidized cellulose, was tested. testing by dilution and diffusion methods was conducted on a spectrum of 27 select microorganisms, including also antibiotic-resistant strains. okcel® h-d showed inhibitory effects on nearly all tested bacteria. in testing using the dilution suspension method, the majority of bacteria showed decrease in cell density by 7-8 orders of magnitude after just 6 h of exposure. ... | 2017 | 28597345 |
| investigation into the antimicrobial action and mechanism of a novel endogenous peptide β-casein 197 from human milk. | a novel endogenous peptide cleaved from 197-213 aa of β-casein, named β-casein 197, was identified by tandem mass spectrometry. β-casein 197 constituted a significant proportion of the peptide content in preterm milk. this study investigated the antibacterial effects and mechanisms against common pathogenic bacteria. six bacterial strains were selected for this study: escherichia coli, staphylococcus aureus, yersinia enterocolitica, listeria monocytogenes, klebsiella pneumonia and bacillus subti ... | 2017 | 28591979 |
| two complementary approaches to quantify variability in heat resistance of spores of bacillus subtilis. | realistic prediction of microbial inactivation in food requires quantitative information on variability introduced by the microorganisms. bacillus subtilis forms heat resistant spores and in this study the impact of strain variability on spore heat resistance was quantified using 20 strains. in addition, experimental variability was quantified by using technical replicates per heat treatment experiment, and reproduction variability was quantified by using two biologically independent spore crops ... | 2017 | 28478320 |
| development of an isothermal amplification-based assay for the rapid visual detection of salmonella bacteria. | the efficient and timely detection of pathogens is a major concern worldwide. the aim of this study was to establish a rapid detection method for salmonella bacteria in food samples to facilitate timely treatment. widely used detection methods currently include culture-based methods and pcr-based methods. the former are time consuming, requiring 2 to 3 d, whereas the latter have higher accuracy but are typically complicated, requiring expertise and expensive instruments. in this study, a sensiti ... | 2017 | 28711269 |
| a rapid salmonella detection method involving thermophilic helicase-dependent amplification and a lateral flow assay. | salmonella is a major foodborne pathogen that is widespread in the environment and can cause serious human and animal disease. since conventional culture methods to detect salmonella are time-consuming and laborious, rapid and accurate techniques to detect this pathogen are critically important for food safety and diagnosing foodborne illness. in this study, we developed a rapid, simple and portable salmonella detection strategy that combines thermophilic helicase-dependent amplification (thda) ... | 2017 | 28526589 |
| inactivation of viruses and bacteria on strawberries using a levulinic acid plus sodium dodecyl sulfate based sanitizer, taking sensorial and chemical food safety aspects into account. | the efficacy of levulinic acid (lva) in combination with sodium dodecyl sulfate (sds) in removal of foodborne viruses, enteric bacterial pathogens and their surrogates on fresh strawberries was investigated. inoculated strawberries were treated with potable water, sodium hypochlorite solution (50ppm), 0.5% lva plus 0.5% sds solution, and 5% lva plus 2% sds solution respectively for 2min, followed by spray-rinsing with potable water. water washing removed at least 1.0-log of the tested viral and ... | 2017 | 28668727 |
| emerging microbiota during cold storage and temperature abuse of ready-to-eat salad. | introduction: ready-to-eat (rte) leafy vegetables have a natural leaf microbiota that changes during different processing and handling steps from farm to fork. the objectives of this study were (i) to compare the microbiota of rte baby spinach and mixed-ingredient salad before and after seven days of storage at 8°c or 15°c; (ii) to explore associations between bacterial communities and the foodborne pathogens listeria monocytogenes, pathogenic yersinia enterocolitica, and pathogen model organism ... | 2017 | 28649305 |
| slightly acidic electrolyzed water combined with chemical and physical treatments to decontaminate bacteria on fresh fruits. | effect of sequential combination of slightly acidic electrolyzed water (saew) with chemical and physical treatments on bacterial decontamination on fruits was investigated in this study. effect of treatments on microbial and sensory quality was also analyzed after subsequent storage at 4 °c and room temperature (rt, 23 ± 0.15 °c). whole apple and tomato fruits were inoculated with cocktail strains of escherichia coli o157:h7 and listeria monocytogenes. uninoculated and inoculated fruits were was ... | 2017 | 28648298 |
| inactivation of bacterial biofilms by visible light-activated unmodified zno nanorods. | various zinc oxide (zno) nanostructures are widely used for photocatalytic antibacterial applications. since zno possess a wide bandgap, it is believed that only uv light may efficiently assist bacterial inactivation and diverse crystal lattice modifications should be applied in order to narrow the bandgap for efficient visible light absorption. in this work we show that even unmodified zno nanorods grown by aqueous chemical growth technique were found to possess intrinsic defects which can be a ... | 2017 | 28628486 |
| inactivation of nonpathogenic escherichia coli, escherichia coli o157:h7, salmonella enterica typhimurium, and listeria monocytogenes in ice using a uvc light-emitting diode. | ice, widely used in the food industry, is a potential cause of food poisoning resulting from microbial contamination. direct microbial inactivation of ice is necessary because microorganisms may have been present in the source water used to make it and/or may have been introduced due to poor hygiene during production or handling of the ice. nonthermal and nondestructive microbial inactivation technologies are needed to control microorganisms in ice. we evaluated the applicability of a uvc light- ... | 2017 | 28621585 |
| sensitive detection of staphylococcus aureus with vancomycin-conjugated magnetic beads as enrichment carriers combined with flow cytometry. | a novel sandwich strategy was designed to detect staphylococcus aureus. the strategy is based on an antibacterial agent that captures bacterial cells and a fluorescein-labeled antibody that acts as the signal-output probe. vancomycin (van), which exerts a strong antibacterial effect on gram-positive bacteria, was utilized as a molecular recognition agent to detect pathogenic bacteria. to effectively concentrate s. aureus, we used bovine serum albumin (bsa) as the amplification carrier to modify ... | 2017 | 28590745 |
| bactericidal effect of 266 to 279nm wavelength uvc-leds for inactivation of gram positive and gram negative foodborne pathogenic bacteria and yeasts. | recently, uvc-led technology has been validated as an alternative to irradiation with conventional mercury uv lamps. in this study, we sought to determine primary factors affecting reduction trends shown in several microorganisms. four major foodborne pathogens (escherichia coli o157:h7, salmonella spp. listeria monocytogenes, staphylococcus aureus) and spoilage yeasts (saccharomyces pastorianus, pichia membranaefaciens), important to the brewing industry, were inoculated onto selective and non- ... | 2017 | 28578053 |
| evaluation of different solvents to extract antibacterial compounds from jalapeño peppers. | chili peppers (capsicum spp.) may possess antibacterial properties and have potential to be used in foods as antimicrobial. the complete chili pepper extract should be evaluated to determine which compounds are responsible for the antimicrobial activity. extraction of compounds from the pepper is completed using a solvent. the type of solvent used for extraction influences which compounds are isolated, therefore the best extraction method needs to be determined. the purpose of this study was to ... | 2017 | 28572934 |
| production and characterization of a new biodegradable fenugreek seed gum based active nanocomposite film reinforced with nanoclays. | in the present work, fenugreek seed gum (fsg)/clay nanocomposite films were prepared with nanoclays (na(+) montmorillonite [mmt], halloysite [hnt] and nanomer(®) i.44 p [nm]) at different amounts (0, 2.5, 5.0 and 7.5g clay/100g fsg) by solution casting method and characterized. increasing amount of nanoclay significantly (p<0.05) improved oxygen barrier and thermal properties of the biodegradable films. agar diffusion tests revealed that fsg based nanocomposite films exhibited strong antimicrobi ... | 2017 | 28536016 |
| investigation of optimum ohmic heating conditions for inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes in apple juice. | control of foodborne pathogens is an important issue for the fruit juice industry and ohmic heating treatment has been considered as one of the promising antimicrobial interventions. however, to date, evaluation of the relationship between inactivation of foodborne pathogens and system performance efficiency based on differing soluble solids content of apple juice during ohmic heating treatment has not been well studied. this study aims to investigate effective voltage gradients of an ohmic heat ... | 2017 | 28525985 |
| development of a gold nanoparticle-based universal oligonucleotide microarray for multiplex and low-cost detection of foodborne pathogens. | bacterial foodborne diseases remain major threats to food safety and public health, especially in developing countries. in this study a novel assay, combining gold nanoparticle (gnp)-based multiplex oligonucleotide ligation-pcr and universal oligonucleotide microarray technology, was developed for inexpensive, specific, sensitive, and multiplex detection of eight common foodborne pathogens, including shigella spp., campylobacter jejuni, bacillus cereus, escherichia coli o157:h7, listeria monocyt ... | 2017 | 28505584 |
| potential of cinnamon oil emulsions as alternative washing solutions of carrots. | the objective of this study was to evaluate the potential of cinnamon oil emulsions as alternative washing solutions to improve the microbial safety of carrots. whey protein concentrate (wpc), gum arabic (ga), lecithin, and their combinations were used to prepare cinnamon oil emulsions. the emulsions were characterized for their hydrodynamic diameter (dh) during 7 days of storage and their antimicrobial activity against cocktails of salmonella enterica , escherichia coli o157:h7, and listeria mo ... | 2017 | 28467186 |
| molecular characterization of silver-stearate nanoparticles (agstnps): a hydrophobic and antimicrobial material against foodborne pathogens. | in this study, silver-stearate nanoparticles (agstnps) have been produced from silver nitrate solutions by replacing the nitrate by stearate ions and then reducing by thermal treatment. agstnps were characterized by particle size, zeta-potential, atr-ftir, contact angle and sem (scanning electron microscopy) analyses. the mean particle size and zeta potential of agstnps were determined to be 69.22±7.30nm and +8.02±0.88mv. atr-ftir analysis showed characteristic ir bands of stearate, revealing th ... | 2015 | 28455024 |
| capability of exopolysaccharide-producing lactobacillus paraplantarum bgcg11 and its non-producing isogenic strain nb1, to counteract the effect of enteropathogens upon the epithelial cell line ht29-mtx. | the putative protective role of the exopolysaccharide (eps)-producing lactobacillus paraplantarum bgcg11, and its non-eps-producing isogenic strain nb1, was tested upon ht29-mtx monolayers challenged with seven opportunistic pathogens. the probiotic strain lactobacillus rhamnosus lmg18243 (gg) was used as a reference bacterium. tested lactobacilli were able to efficiently reduce the attachment to ht29-mtx of most pathogens. lb. paraplantarum nb1 and lb. rhamnosus gg were more efficient reducing ... | 2015 | 28411984 |
| the first report on listeria monocytogenes producing siderophores and responds positively to n-acyl homoserine lactone (ahl) molecules by enhanced biofilm formation. | listeria monocytogenes are gram-positive well-known emerging food-borne pathogens causing listeriosis in humans. in the present study, we have isolated biofilm-forming listeria sp. from utensils used by a local milk collection dairy society at usgao goa, which collects milk for goa dairy. through biochemical tests and 16s rrna sequence analysis, the bacterium was confirmed to be l. monocytogenes and designated as strain bn3, having genbank accession number mf095110. we report for the first time ... | 2017 | 28762063 |
| next generation sequencing-based multigene panel for high throughput detection of food-borne pathogens. | contamination of food by chemicals or pathogenic bacteria may cause particular illnesses that are linked to food consumption, commonly referred to as foodborne diseases. bacteria are present in/on various foods products, such as fruits, vegetables and ready-to-eat products. bacteria that cause foodborne diseases are known as foodborne pathogens (fbps). accurate detection methods that are able to reveal the presence of fbps in food matrices are in constant demand, in order to ensure safe foods wi ... | 2017 | 28578266 |
| two novel roles of buffalo milk lactoperoxidase, antibiofilm agent and immunomodulator against multidrug resistant salmonella enterica serovar typhi and listeria monocytogenes. | the increasing occurrence of multidrug resistant bacteria causing bacteremia infection, constitutes a major health problem, difficult-to-treat bacteremia due to its ability to form biofilm. buffalo milk lactoperoxidase (bmlpo) is effective and safe to use as bacteriostatic agent. the mic of bmlpo and amikacin were used to evaluate the antibiofilm activity against resistant l. monocytogenes and s. typhi. prophylactic effects of bmlpo against l. monocytogenes and s. typhi bacteremia in vivo have b ... | 2017 | 28587762 |
| antibacterial effects of lactobacillus isolates of curd and human milk origin against food-borne and human pathogens. | this study was undertaken to assess the antibacterial efficacy of lactobacilli isolated from curd and human milk samples. identities of thirty-one different lactobacilli (20 from curd and 11 from human milk) were confirmed by genus-specific pcr and 16s rrna-based sequencing. these strains belonged to five species, lactobacillus casei, l. delbrueckii, l. fermentum, l. plantarum, and l. pentosus. antibacterial activities of cell-free supernatants (cfss) of all the lactobacillus isolates were estim ... | 2017 | 28401466 |
| characterization of antimicrobial properties of salmonella phage felix o1 and listeria phage a511 embedded in xanthan coatings on poly(lactic acid) films. | beyond simply providing a barrier between food and external contaminants, active packaging technologies aim to inhibit pathogen survival and growth within the packaged environment. bacteriophages have a proven track record as targeted antimicrobials but have yet to be successfully integrated in active packaging without serious loss of activity. we have developed two bacteriophage based xanthan coatings on poly(lactic acid) (pla) film which significantly inhibits salmonella typhimurium and lister ... | 2017 | 28576360 |
| isolation and characterization of listeria monocytogenes from the black-headed gull feces in kunming, china. | listeria monocytogenes is among the most important foodborne pathogens, which can be isolated from bird feces. the aim of this study was to examine the prevalence, genotypes, virulence factors and drug-resistance characteristics of l. monocytogenes isolated from the black-headed gull in kunming, china. serotypes and virulence genes were identified by polymerase chain reaction. pulsed-field gel electrophoresis (pfge) and multi-locus sequence typing (mlst) methods were used to describe the genetic ... | 2017 | 28473260 |
| efficacy of radiant catalytic ionization to reduce bacterial populations in air and on different surfaces. | air contamination by biological agents is often observed in medical or veterinary facilities and industrial plants. bioaerosols may sediment and pose the surface contamination. microorganisms present on them may become a source of infections among humans and food contamination. this study determined the use of oxidative gases, including ozone and peroxide, generated by the radiant catalytic ionization (rci) cell for the inactivation of acinetobacter baumannii, escherichia coli, enterococcus faec ... | 2017 | 28803189 |
| microorganisms of the intestinal microbiota of oncorhynchus mykiss produce antagonistic substances against bacteria contaminating food and causing disease in humans. | the objective was to analyse the antibacterial ability of members of the intestinal microbiota of oncorhynchus mykiss specimens on several bacterial target strains that contaminate food and cause disease in humans. bacterial colonies from an intestinal portion of the 20 specimens of o. mykiss were obtained in different culture media. several of the colonies showed antibacterial action on different target strains. the bacterial species with the highest antagonistic capacity were hafnia alvei and ... | 2017 | 28713788 |
| the antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human hel 12469 cells. | six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. antimicrobial activity was shown against pathogenic (escherichia coli, salmonella typhimurium, yersinia enterocolitica, staphylococcus aureus, listeria monocytogenes, and enterococcus faecalis) and environmental bacteria (bacillus cereus, arthrobacter protophormiae, pseudomonas fragi) and fungi (chaetomium globosum, penicillium chrysogenum, cladospori ... | 2017 | 28811611 |
| production of recombinant antimicrobial polymeric protein beta casein-e 50-52 and its antimicrobial synergistic effects assessment with thymol. | accelerating emergence of antimicrobial resistance among food pathogens and consumers' increasing demands for preservative-free foods are two contemporary challenging aspects within the food industry. antimicrobial packaging and the use of natural preservatives are promising solutions. in the present study, we used beta-casein-one of the primary self-assembly proteins in milk with a high polymeric film production capability-as a fusion partner for the recombinant expression of e 50-52 antimicrob ... | 2017 | 28561787 |
| antimicrobial activity and chemical composition of brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide. | brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. however, studies on the antimicrobial activity of brunfelsia uniflora flower oleoresin have not been found yet. this study aimed to evaluate the chemical composition and antimicrobial activity of b. uniflora flower oleoresin obtained by supercritical carbon dioxide. oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition ... | 2017 | 28407186 |
| biogenic fabrication of cunps, cu bioconjugates and in vitro assessment of antimicrobial and antioxidant activity. | in the present study, the authors synthesised copper nanoparticles (cunps) by using extract of zingiber officinale (ginger) and later the nps were bioconjugated with nisin, which shows antimicrobial activity against food spoilage microorganisms. cunps and its bioconjugate were characterised by ultraviolet-vis spectroscopy, np tracking analysis, zetasizer, transmission electron microscopy analysis, x-ray diffraction and fourier transform infra-red (ftir) spectroscopy. zeta potential of cunps and ... | 2017 | 28745291 |
| fungal chitosan and lycium barbarum extract as anti-listeria and quality preservatives in minced catfish. | listeria monocytogenes is a foodborne bacterial pathogen that causes serious health risks. chitosan (ch) is a bioactive polymer that could be effectively applied for foodstuffs biopreservation. lycium barbarum (goji berry) is ethnopharmaceutical fruit that have diverse health protecting benefits. chitosan was produced from a. niger and employed with l. barbarum extract (lbe) as blends for listeria control and quality biopreservation of african catfish mince (clarias gariepinus). chitosan could u ... | 2017 | 28655659 |
| holographic deep learning for rapid optical screening of anthrax spores. | establishing early warning systems for anthrax attacks is crucial in biodefense. despite numerous studies for decades, the limited sensitivity of conventional biochemical methods essentially requires preprocessing steps and thus has limitations to be used in realistic settings of biological warfare. we present an optical method for rapid and label-free screening of bacillus anthracis spores through the synergistic application of holographic microscopy and deep learning. a deep convolutional neur ... | 2017 | 28798957 |
| phenolic acids and flavonoids of peanut by-products: antioxidant capacity and antimicrobial effects. | peanut skin (ps) and meal from dry-blanched peanuts (mdbp) were evaluated as sources of phenolic compounds. ps rendered the highest total phenolic content, antioxidant capacity towards abts radical cation, dpph and hydroxyl radicals as well as reducing power. phenolic acids were present in ps and mdbp whereas proanthocyanidins and monomeric flavonoids were found only in ps as identified by hplc-dad-esi-ms(n). procyanidin-rich extracts prevented oxidation in non-irradiated and gamma-irradiated fi ... | 2017 | 28764032 |
| detection of eight foodborne bacterial pathogens by oligonucleotide array hybridization. | simultaneous and rapid detection of multiple foodborne bacterial pathogens is important for the prevention of foodborne illnesses. | 2017 | 28713514 |
| implications of neuroinvasive bacterial peptides on rodents behaviour and neurotransmission. | neuroinvasive microbes are capable of applying their influences on the autonomic nervous system (ans) of the host followed by the involvement of central nervous system (cns) by releasing extracellular metabolites that may cause alterations in the biochemical and neurophysiological environment. consequently synaptic, neuroendocrine, peripheral immune, neuro-immune, and behavioural responses of the host facilitate the progression of infection. the present study was designed to extrapolate the effe ... | 2017 | 28671588 |
| isolation and preliminary characterization of a bacteriocin-producer bacillus strain inhibiting methicillin resistant staphylococcus aureus. | in a multivalent approach to discover new antimicrobial substance, a total of 160 bacilli were isolated from termitarium soil, characterized on the basis of their morphological and physiological characters and screened for their antimicrobial activity by agar well diffusion method against certain drug resistant pathogenic bacteria such as staphylococcus aureus, methicillin resistant staphylococcus aureus and common food contaminating bacteria listeria monocytogenes. after preliminary screening, ... | 2017 | 28605978 |
| use of whey as a culture medium for bacillus clausii for the production of protein hydrolysates with antimicrobial and antioxidant activity. | the use of whey as a natural culture medium to produce hydrolysates with antimicrobial from bacillus clausii is the objective of this research. the fermentation process was carried out at 25 ℃ for five days, where proteolytic activity, soluble peptides quantification, and antimicrobial activity using the disc diffusion method were determined every day. the fermented whey reached values of proteolytic activity between 90 and 260 u ml(-1) and a production of soluble peptides between 100 and 2070 ... | 2017 | 28816527 |
| utilization of industrial waste for the production of bio-preservative from bacillus licheniformis me1 and its application in milk and milk-based food products. | the bio-preservative efficacy of a partially purified antibacterial peptide (ppabp) produced by bacillus licheniformis me1 in an economical medium developed using agro-industry waste was evaluated by direct application in milk and milk-based food products. the addition of ppabp in milk samples stored at 4 ± 2 °c and 28 ± 2 °c resulted in the growth inhibition of pathogens listeria monocytogenes scott a, micrococcus luteus atcc 9341, and staphylococcus aureus fri 722. the shelf life of milk sampl ... | 2017 | 28842843 |
| lactolisterin bu, a novel class ii broad spectrum bacteriocin from lactococcus lactis subsp. lactis bv. diacetylactis bgbu1-4. | lactococcus lactis subsp. lactis bv. diacetylactis bgbu1-4 produces a novel bacteriocin, lactolisterin bu, with strong antimicrobial activity against many species of gram-positive bacteria, including important food spoilage and food-borne pathogens such as listeria monocytogenes, staphylococcus aureus, bacillus sp. and streptococci. lactolisterin bu was extracted from the cell surface of bgbu1-4 by propan-2-ol and purified to homogeneity by c18 solid phase extraction and reversed phase hplc. the ... | 2017 | 28842543 |
| in vitro antimicrobial activity screening of xylaria hypoxylon. | fungi have a potential of using both as nutritive and medicinal food stuff. because of containing several therapeutic agents, they are reported to be used for hundreds of years to treat several diseases caused by bacteria, fungi, viruses and parasites. the aim of this study is to determine the in vitro antimicrobial activity of xylaria hypoxylon, which were collected from yomra, trabzon, turkey. | 2016 | 28852719 |
| chemical composition of mentha pulegium and rosmarinus officinalis essential oils and their antileishmanial, antibacterial and antioxidant activities. | the aim of the study was the determination of the chemical composition of mentha pulegium l. and rosmarinus officinalis l. essential oils and the evaluation of their antileishmanial, antibacterial and antioxidant activities. essential oils (eos) were isolated using steam distillation and the chemical composition was determined using gc-ms analysis. the antibacterial activity was tested against ten pathogenic strains using the diffusion method, the minimum inhibitory concentration (mic) and the m ... | 2017 | 28821401 |
| replication-transcription conflicts generate r-loops that orchestrate bacterial stress survival and pathogenesis. | replication-transcription collisions shape genomes, influence evolution, and promote genetic diseases. although unclear why, head-on transcription (lagging strand genes) is especially disruptive to replication and promotes genomic instability. here, we find that head-on collisions promote r-loop formation in bacillus subtilis. we show that pervasive r-loop formation at head-on collision regions completely blocks replication, elevates mutagenesis, and inhibits gene expression. accordingly, the ac ... | 2017 | 28802046 |
| probiotic characteristics of lactobacillus curvatus dn317, a strain isolated from chicken ceca. | the probiotic characteristics of lactobacillus curvatus dn317, a strain isolated from chicken ceca, were evaluated. this strain was selected for study from the isolated lactobacillus strains because it has specific anti-microbial activity against campylobacter jejuni atcc 33560, camp. jejuni nctc 11168, listeria monocytogenes atcc 7644, and bacillus subtilis atcc 8633. lact. curvatus dn317 showed an auto-aggregation percentage of 72% and presented the highest co-aggregation with lact. monocytoge ... | 2017 | 28741151 |
| imipenem/cilastatin : a reappraisal of its antibacterial activity, pharmacokinetic properties and therapeutic efficacy. | imipenem is an antibacterial agent of the carbapenem class of β-lactams, with a very broad spectrum of activity that includes most gram-negative and gram-positive pathogens, aerobes and anaerobes, and with marked activity against species producing β-lactamases. it is coadministered with cilastatin, a renal dehydropeptidase inhibitor that prevents renal metabolism of imipenem. as initial monotherapy, imipenem/cilastatin provides effective and well-tolerated treatment of moderate to severe infecti ... | 1992 | 28634918 |
| getting "inside" type i ifns: type i ifns in intracellular bacterial infections. | type i interferons represent a unique and complex group of cytokines, serving many purposes during innate and adaptive immunity. discovered in the context of viral infections, type i ifns are now known to have myriad effects in infectious and autoimmune disease settings. type i ifn signaling during bacterial infections is dependent on many factors including whether the infecting bacterium is intracellular or extracellular, as different signaling pathways are activated. as such, the repercussions ... | 2017 | 28529959 |
| irrigation water sources and time intervals as variables on the presence of campylobacter spp. and listeria monocytogenes on romaine lettuce grown in muck soil. | irrigation water has been identified as a possible source of vegetable contamination by foodborne pathogens. risk management for pathogens such as campylobacter spp. and listeria monocytogenes in fields can be influenced by the source of the irrigation water and the time interval between last irrigation and harvest. plots of romaine lettuce were irrigated with manure-contaminated water or aerated pond water 21, 7, or 3 days prior to harvesting, and water and muck soil samples were collected at e ... | 2017 | 28604176 |
| postchill antimicrobial treatments to control salmonella, listeria, and campylobacter contamination on chicken skin used in ground chicken. | ground poultry products are frequently contaminated with foodborne pathogens. with the potential for increased regulatory scrutiny, it is important to use sufficient intervention strategies to control pathogen levels effectively. a large proportion of the bacteria introduced to ground chicken are likely to come from broiler skin, which is added to achieve target fat content and maintain product texture and taste. in this research, antimicrobials, including 50 ppm of chlorine and 1,200 ppm of per ... | 2017 | 28414258 |
| rnaseq reveals complex response of campylobacter jejuni to ovine bile and in vivo gallbladder environment. | colonization of the gallbladder by enteric pathogens such as salmonella typhi, listeria monocytogenes, and campylobacter jejuni is thought to play a key role in transmission and persistence of these important zoonotic agents; however, little is known about the molecular mechanisms that allow for bacterial survival within this harsh environment. recently, a highly virulent c. jejuni sheep abortion (sa) clone represented by the clinical isolate ia3902 has emerged as the dominant cause for sheep ab ... | 2017 | 28611744 |
| protein kinase c-delta (pkcδ), a marker of inflammation and tuberculosis disease progression in humans, is important for optimal macrophage killing effector functions and survival in mice. | we previously demonstrated that protein kinase c-δ (pkcδ) is critical for immunity against listeria monocytogenes, leishmania major, and candida albicans infection in mice. however, the functional relevance of pkcδ during mycobacterium tuberculosis (mtb) infection is unknown. pkcδ was significantly upregulated in whole blood of patients with active tuberculosis (tb) disease. lung proteomics further revealed that pkcδ was highly abundant in the necrotic and cavitory regions of tb granulomas in mu ... | 2017 | 28832027 |
| purification of leucocin a for use on wieners to inhibit listeria monocytogenes in the presence of spoilage organisms. | the aims of this study were to improve the method for purification of leucocin a to increase yield of peptide and to evaluate the efficacy of leucocin a and an analogue of leucocin a (leucocin n17l) to inhibit the growth of listeria monocytogenes on wieners in the presence of spoilage organisms. leucocin a was produced by leuconostoc gelidum ual187 and purified with a five-fold increase in yield; leucocin n17l was synthesized replacing asparagine at residue 17 with leucine. five strains of l. mo ... | 2017 | 28570918 |
| tpl2 promotes innate cell recruitment and effector t cell differentiation to limit citrobacter rodentium burden and dissemination. | tumor progression locus 2 (tpl2) is a serine-threonine kinase that regulates th1 differentiation, secretion of the inflammatory cytokine ifnγ, and host defense against the intracellular pathogens toxoplasma gondii, listeria monocytogenes, and mycobacterium tuberculosis however, there is relatively little known about the contribution of tpl2 to th17 differentiation and immune cell function during infection with an extracellular pathogen. the goal of this study was to determine whether tpl2 influe ... | 2017 | 28760932 |
| selection of surrogate bacteria for use in food safety challenge studies: a review. | nonpathogenic surrogate bacteria are prevalently used in a variety of food challenge studies in place of foodborne pathogens such as listeria monocytogenes, salmonella, escherichia coli o157:h7, and clostridium botulinum because of safety and sanitary concerns. surrogate bacteria should have growth characteristics and/or inactivation kinetics similar to those of target pathogens under given conditions in challenge studies. it is of great importance to carefully select and validate potential surr ... | 2017 | 28805457 |
| vaginal self-sampling as a diagnosis tool in low-income countries and potential applications for exploring the infectious causes of miscarriage. | gynecological health is a challenge in low-income countries. personal opposition to perineal examination has been overcome by the use of vaginal self-sampling. here, we review the use of this procedure notably in low-income countries and the main infectious causes of miscarriage. vaginal self-sampling was mainly used for human papillomavirus detection but also to detect microorganisms causing sexually transmitted infections or bacterial vaginosis. 58 studies have been performed in low-resource c ... | 2017 | 28604063 |
| exploitation of spr to investigate the importance of glycan chains in the interaction between lactoferrin and bacteria. | bovine lactoferrin (lf) has been shown to prevent adhesion to and invasion of mammalian cell lines by pathogenic bacteria, with evidence for direct bacterial binding by the milk glycoprotein. however, the glycosylation pattern of lf changes over the lactation cycle. in this study, we aim to investigate the effect that this variation has on the milk glycoprotein's ability to interact with pathogens. surface plasmon resonance technology was employed to compare the binding of lf from colostrum (ear ... | 2017 | 28653977 |
| the epidemiology, management, and outcomes of bacterial meningitis in infants. | the pathogens that cause bacterial meningitis in infants and their antimicrobial susceptibilities may have changed in this era of increasing antimicrobial resistance, use of conjugated vaccines, and maternal antibiotic prophylaxis for group b streptococcus (gbs). the objective was to determine the optimal empirical antibiotics for bacterial meningitis in early infancy. | 2017 | 28600447 |
| clec5a is a critical receptor in innate immunity against listeria infection. | the c-type lectin member 5a (clec5a) is a pattern recognition receptor for members of the flavivirus family and has critical functions in response to dengue virus and japanese encephalitis virus. here we show that clec5a is involved in neutrophil extracellular trap formation and the production of reactive oxygen species and proinflammatory cytokines in response to listeria monocytogenes. inoculation of clec5a (-/-) mice with l. monocytogenes causes rapid bacterial spreading, increased bacterial ... | 2017 | 28824166 |
| characterization of enterococcus durans 152 bacteriocins and their inhibition of listeria monocytogenes in ham. | listeria monocytogenes is a nonfastidious, widely occurring foodborne pathogen that is a major challenge to the food industry. enterococcus durans 152, a confirmed l. monocytogenes-control microorganism, was isolated from floor drain samples from a food processing facility. in this study, the two bacteriocins produced by e. durans 152 were characterized and identified as dur 152a (an enterocin l50a derivative with two amino acid substitutions of i→m) and enterocin l50b. the bacteriocins were the ... | 2017 | 28800831 |
| characterization and application of antilisterial enterocins on model fresh cheese. | enterococcus faecalis strains isolated from an artisanal cheese were selected based on enterocin production against listeria monocytogenes. the strains formed biofilms and presented high hydrophobic character and good autoaggregation and coaggregation capacity with l. monocytogenes. strains l3a21m3 and l3b1k3 presented high survival under gastrointestinal conditions, were able to adhere to human intestinal cells (caco-2 and ht-29), and blocked the adhesion and invasion of l. monocytogenes. the a ... | 2017 | 28703625 |
| signal biosynthesis inhibition with ambuic acid as a strategy to target antibiotic-resistant infections. | there has been major interest by the scientific community in antivirulence approaches against bacterial infections. however, partly due to a lack of viable lead compounds, antivirulence therapeutics have yet to reach the clinic. here we investigate the development of an antivirulence lead targeting quorum sensing signal biosynthesis, a process that is conserved in gram-positive bacterial pathogens. some preliminary studies suggest that the small molecule ambuic acid is a signal biosynthesis inhi ... | 2017 | 28607020 |
| testing commercial biopreservative against spoilage microorganisms in map packed ricotta fresca cheese. | ricotta fresca cheese is susceptible to secondary contamination and is able to support the growth of pathogens or spoilage psychotrophic bacteria during storage. the aim of the present study was to evaluate which among three commercial biopreservatives was suitable to be used to control the growth of spoilage microorganisms in sheep's milk map ricotta fresca cheese. 144 ricotta fresca cheese samples were inoculated either with the bioprotective culture lyofast fpr 2 (including enterococcus faeci ... | 2017 | 28576375 |
| bacteriocin-producing enterococcus faecium lcw 44: a high potential probiotic candidate from raw camel milk. | bacterial isolates from raw camel milk were screened for antibacterial activity using the agar diffusion assay. ten isolates selected for their inhibition of gram-positive bacteria were identified by 16s sequencing as enterococcus faecium or durans. an isolate named e. faecium lcw 44 exhibited the broadest antibacterial spectrum with an inhibitory activity against several gram-positive strains belonging to the genera clostridium, listeria, staphylococcus, and lactobacillus. e. faecium lcw 44 was ... | 2017 | 28572793 |
| application of nanocompostie chitosan and carboxymethyl cellulose films containing natural preservative compounds in minced camel's meat. | in the present study, novel films based on nanomontmorillonite-chitosan (mmt-ch) and nanomontmorillonite-carboxymethyl cellulose (mmt-cmc) incorporated with different concentrations of ziziphora clinopodioides essential oil (zeo; 0.5, 1 and 2%) alone and in combination with ficus carica extract (fch; 1%) were investigated as active packaging materials for minced camel's meat to increase the shelf life (microbial, chemical and sensory properties) and inhibit the growth of listeria monocytogenes a ... | 2017 | 28847602 |
| impact of the reusing of food manufacturing wastewater for irrigation in a closed system on the microbiological quality of the food crops. | in order to evaluate if the reuse of food industry treated wastewater is compatible for irrigation of food crops, without increased health risk, in the present study a cropping system, in which ground water and treated wastewater were used for irrigation of tomato and broccoli, during consecutive crop seasons was monitored. water, crop environment and final products were monitored for microbial indicators and pathogenic bacteria, by conventional and molecular methods. the microbial quality of th ... | 2017 | 28843124 |
| in vitro characterization of lactobacillus strains isolated from fruit processing by-products as potential probiotics. | nine wild lactobacillus strains, namely lactobacillus plantarum 53, lactobacillus fermentum 56, l. fermentum 60, lactobacillus paracasei 106, l. fermentum 250, l. fermentum 263, l. fermentum 139, l. fermentum 141, and l. fermentum 296, isolated from fruit processing by-products were evaluated in vitro for a series of safety, physiological functionality, and technological properties that could enable their use as probiotics. considering the safety aspects, the resistance to antibiotics varied amo ... | 2017 | 28836171 |
| simultaneous detection of escherichia coli o157:h7, salmonella enteritidis, and listeria monocytogenes at a very low level using simultaneous enrichment broth and multichannel spr biosensor. | detection of foodborne pathogens at very low levels is still a challenge. a custom-built multichannel surface plasmon resonance (spr) biosensor and simultaneous enrichment broth (seb) were used to develop a simultaneous detection method for 3 important foodborne pathogens, escherichia coli o157:h7 (o157:h7), salmonella enteritidis, and listeria monocytogenes, at a very low level. these 3 foodborne pathogens at a very low level (14, 6, and 28 cfu/25 g (ml) for o157:h7, s. enteritidis, and l. mono ... | 2017 | 28833106 |
| synthesis and antimicrobial studies of new antibacterial azo-compounds active against staphylococcus aureus and listeria monocytogenes. | some novel (phenyl-diazenyl)phenols (4a-m) were designed and synthesized to be evaluated for their antibacterial activity. starting from an active previously-synthesized azobenzene chosen as lead compound, we introduced some modifications and optimization of the structure, in order to improve solubility and drug conveyance. structures of all newly-synthesized compounds were confirmed by ¹h nuclear magnetic resonance (nmr), mass spectrometry, and uv-vis spectroscopy. antibacterial activity of the ... | 2017 | 28825625 |
| comparison of the microbiological quality and safety between conventional and organic vegetables sold in malaysia. | given the remarkable increase of public interest in organic food products, it is indeed critical to evaluate the microbiological risk associated with consumption of fresh organic produce. organic farming practices including the use of animal manures may increase the risk of microbiological contamination as manure can act as a vehicle for transmission of foodborne pathogens. this study aimed to determine and compare the microbiological status between organic and conventional fresh produce at the ... | 2017 | 28824567 |
| a suicidal strain of listeria monocytogenes is effective as a dna vaccine delivery system for oral administration. | in this study we determined the in vivo activity of model ovalbumin vaccines delivered by direct intramuscular delivery of plasmid dna or oral delivery using a recombinant suicidal listeria monocytogenes strain (rsδ2). in a previous report we described how rsδ2 is capable of delivering luciferase, as protein or dna, in vitro, into non-dividing intestinal epithelial cells (kuo et al., 2009). this is achieved by engineering a dual expression shuttle vector, pdulx-luc, that replicates in e. coli an ... | 2017 | 28822642 |
| preparation, characterization and antibacterial activity of oxidized κ-carrageenan. | the oxidized κ-carrageenans with different oxidation levels were prepared through the hydrogen peroxide and copper sulfate redox system. the oxidation level of oxidized κ-carrageenan was successfully controlled by adjusting the dosage of hydrogen peroxide. the results showed that the microtopography of oxidized κ-carrageenan changed from rough granules to smooth flakes, mainly resulting from the easily melting property of oxidized κ-carrageenan induced by introduced carboxyl and aldehyde groups. ... | 2017 | 28821027 |
| short communication: detection of stx2 and elt genes in bovine milk by using a multiplex pcr system. | the aim of this study was to detect 2 important toxin genes from diarrheagenic escherichia coli (dec) in bovine milk using a new multiplex pcr. to standardize the multiplex pcr, the stx2 and elt genes were investigated for the detection of shiga toxin-producing escherichia coli (stec) and enterotoxigenic e. coli (etec), respectively. the dna template was prepared with a thermal procedure (boiling) and a commercial kit. samples consisted of uht and pasteurized milk, both skimmed, and stec and ete ... | 2017 | 28803013 |
| comparative analysis of the composition and active property evaluation of certain essential oils to assess their potential applications in active food packaging. | the antifungal, antibacterial, and antioxidant activity of four commercial essential oils (eos) (thyme, clove, rosemary, and tea tree) from romanian production were studied in order to assess them as bioactive compounds for active food packaging applications. the chemical composition of the oils was determined with the folin-ciocâlteu method and gas chromatography coupled with mass spectrometry and flame ionization detectors, and it was found that they respect the afnor/iso standard limits. the ... | 2017 | 28772407 |
| synthesis and in vitro evaluation of peracetyl and deacetyl glycosides of eugenol, isoeugenol and dihydroeugenol acting against food-contaminating bacteria. | essential oils, as well as their separate components, have shown promise as alternatives to synthetic preservatives. therefore, it would be interesting to optimize the effect of these compounds and to evaluate their applicability as additives in food. to this end, six peracetyl and deacetyl glycosides were synthesized from eugenol, isoeugenol and dihydroeugenol. all of the glycosides were characterized by ir and nmr. the synthesized compounds and their aglycones were evaluated to determine their ... | 2017 | 28763946 |
| anti-adhesion and anti-biofilm potential of organosilane nanoparticles against foodborne pathogens. | nowadays, modification of surfaces by nanoparticulate coatings is a simple process that may have applications in reducing the prevalence of bacterial cells both on medical devices and food processing surfaces. to this direction, biofilm biological cycle of salmonella typhimurium, listeria monocytogenes, escherichia coli o157:h7, staphylococcus aureus, and yersinia enterocolitica on stainless steel and glass surfaces, with or without nanocoating was monitored. to achieve this, four different comm ... | 2017 | 28744277 |
| development of antimicrobial packaging film made from poly(lactic acid) incorporating titanium dioxide and silver nanoparticles. | polylactide (pla)/nano-tio₂ and pla/nano-tio₂/nano-ag blends films were prepared by a solvent volatilization method. compared to pure pla film, the nano-blend films have low water vapor permeability (wvp) and a poor transparency. with the increase of the nps in the pla, the tensile strength (ts) and elastic modulus (em) decreased, while the elongation at break (ε) increased. sem analysis indicated a rougher cross-section of the nano-blend films. according to the ftir analysis, no new chemical bo ... | 2017 | 28703753 |
| prevalence and antibiotic resistance profiles of cerebrospinal fluid pathogens in children with acute bacterial meningitis in yunnan province, china, 2012-2015. | acute bacterial meningitis is still considered one of the most dangerous infectious diseases in children. to investigate the prevalence and antibiotic resistance profiles of cerebrospinal fluid (csf) pathogens in children with acute bacterial meningitis in southwest china, csf samples from 179 meningitis patients (3 days to 12 years old) with positive culture results were collected from 2012 to 2015. isolated pathogens were identified using the vitek-32 system. gram stain results were used to gu ... | 2017 | 28662145 |
| effects of heat treatment on the antioxidative and anti-inflammatory properties of orange by-products. | this study investigated the changes in the functional components, antioxidative activities, antibacterial activities, anti-inflammatory activities of orange (citrus sinensis (l.) osbeck) by-products (obp) on heat treatment at 50 and 100 °c (hereafter denoted 50d and 100d extracts, respectively). optimal heating conditions were also investigated. the total phenolic content, flavonoid content and antioxidative activities of obp extracts significantly increased on heat treatment. the lag time of cu ... | 2017 | 28653733 |
| tolerance development in listeria monocytogenes-escherichia coli dual-species biofilms after sublethal exposures to pronase-benzalkonium chloride combined treatments. | this study was designed to assess the effects that sublethal exposures to pronase (prn) and benzalkonium chloride (bac) combined treatments have on listeria monocytogenes-escherichia coli dual-species biofilms grown on stainless steel in terms of tolerance development (td) to these compounds. additionally, fluorescence microscopy was used to observe the changes of the biofilm structure. prn-bac exposure was carried out using three different approaches and td was evaluated treating biofilms with ... | 2017 | 28648294 |
| removal of foodborne pathogen biofilms by acidic electrolyzed water. | biofilms, which are complex microbial communities embedded in the protective extracellular polymeric substances (eps), are difficult to remove in food production facilities. in this study, the use of acidic electrolyzed water (aew) to remove foodborne pathogen biofilms was evaluated. we used a green fluorescent protein-tagged escherichia coli for monitoring the efficiency of aew for removing biofilms, where under the optimal treatment conditions, the fluorescent signal of cells in the biofilm di ... | 2017 | 28638370 |
| bacterial meningitis complicating the course of liver cirrhosis. | meningitis is rarely reported in studies investigating bacterial infections in patients affected by liver cirrhosis. we investigated the findings of bacterial meningitis in patients affected by liver cirrhosis referred to our department in a 16-year period. | 2017 | 28616745 |
| predicting and modelling the growth of potentially pathogenic bacteria in coalho cheese. | coalho is a semihard medium- to high-moisture cheese produced in various states in the northeastern region of brazil. this study was conducted to predict the growth kinetics (maximum growth rate, grmax) of escherichia coli, listeria monocytogenes, salmonella, and staphylococcus aureus using the combase predictor with various combinations of temperature, ph, and water activity (aw) in commercial coalho cheese samples. the growth of two antibiotic-resistant derivative strains of l. monocytogenes ( ... | 2017 | 28604174 |
| efficacy of combined sous vide-microwave cooking for foodborne pathogen inactivation in ready-to-eat chicory stems. | there is a variety of different food processing methods, which can be used to prepare ready-to-eat foods. however, the need to preserve the freshness and nutritional qualities leads to the application of mild technologies which may be insufficient to inactivate microbial pathogens. in this work, fresh chicory stems were packed under a vacuum in films, which were transparent to microwaves. these were then exposed to microwaves for different periods of time. the application of sous vide microwave ... | 2017 | 28597952 |
| presence of human pathogens in produce from retail markets in northern germany. | two hundred fresh produce samples (cucumber, carrots, herbs, leaf lettuce, and ready-to-eat mixed salad leaves) were obtained from retail in northern germany in 2015. these were investigated for microbial contamination and the presence of foodborne pathogens, including listeria monocytogenes, salmonella serovars, presumptive bacillus (b.) cereus, and shiga toxin-producing escherichia coli using culture-dependent (enrichment, plating on selective media) and -independent (real-time polymerase chai ... | 2017 | 28594569 |
| microrna-21 limits uptake of listeria monocytogenes by macrophages to reduce the intracellular niche and control infection. | mirnas are important post-transcriptional regulators of gene expression. mirna expression is a crucial part of host responses to bacterial infection, however there is limited knowledge of their impact on the outcome of infections. we investigated the influence of mir-21 on macrophage responses during infection with listeria monocytogenes, which establishes an intracellular niche within macrophages. mir-21 is induced following infection of bone marrow-derived macrophages (bmdms) with listeria. mi ... | 2017 | 28589100 |
| inhibitor-assisted high-pressure inactivation of bacteria in skim milk. | the combined inactivation effects of high hydrostatic pressure (hhp) and antimicrobial compounds (potassium sorbate and ε-polylysine [ε-pl]) on 4 different bacterial strains present in skim milk and the effect of these treatments on milk quality were investigated in this study. hhp treatment at 500 mpa for 5 min reduced the populations of escherichia coli, salmonella enterica typhimurium, listeria monocytogenes, and staphylococcus aureus from 6.5 log colony-forming units (cfus) or higher to less ... | 2017 | 28556370 |
| incorporating zataria multiflora boiss. essential oil and sodium bentonite nano-clay open a new perspective to use zein films as bioactive packaging materials. | active zein films with different levels of zataria multiflora boiss. essential oil were produced successfully. to enhance properties of this biopolymer for food packaging applications, sodium bentonite clay was used at two levels (2 and 4%). the results indicated that the addition of z. multiflora boiss. essential oil caused a reduction in tensile strength and young's modulus and slight increase in the percent of elongation at break of the films. maximum solubility in water and water vapor perme ... | 2017 | 28549407 |
| surfactin-loaded polyvinyl alcohol (pva) nanofibers alters adhesion of listeria monocytogenes to polystyrene. | surfactin-loaded polyvinyl alcohol (pva) nanofibers were spun using gravity electrospinning. scanning electron microscopy (sem) images showed that nanofibers spun with surfactin are free from bead formation and uniform in diameter. the average nanofiber diameters were decreased (273±39nm, 259±39nm and 217±33nm) with increasing levels of surfactin (0.5, 1.0 and 1.5%, w/v) into pva (10%, w/v). the 10% (w/v) pva had average fiber diameter of 303±33nm. atomic force microscopy (afm) analysis showed t ... | 2017 | 28532029 |
| water for agriculture: the convergence of sustainability and safety. | agricultural water is a precious and limited resource. increasingly more water types and sources are being explored for use in irrigation within the united states and across the globe. as outlined in this chapter, the produce safety rule (psr) in the food safety and modernization act (fsma) provide irrigation water standards for application of water to fruits and vegetables consumed raw. these rules for production and use of water will continue to develop and be required as the world experiences ... | 2017 | 28527182 |
| outbreak-related disease burden associated with consumption of unpasteurized cow's milk and cheese, united states, 2009-2014. | the growing popularity of unpasteurized milk in the united states raises public health concerns. we estimated outbreak-related illnesses and hospitalizations caused by the consumption of cow's milk and cheese contaminated with shiga toxin-producing escherichia coli, salmonella spp., listeria monocytogenes, and campylobacter spp. using a model relying on publicly available outbreak data. in the united states, outbreaks associated with dairy consumption cause, on average, 760 illnesses/year and 22 ... | 2017 | 28518026 |