| heat resistance of listeria monocytogenes in semi-skim milk supplemented with vanillin. | the kinetics of destruction of listeria monocytogenes scott a in semi-skim milk heated at 55, 58, 60 and 62°c without and with addition of 900, 1400 and 1800 ppm of vanillin was studied. survival curves displayed an initial shoulder phase followed by an accelerating killing phase. addition of vanillin to semi-skim milk heated between 55 and 62°c reduced the heat resistance of l. monocytogenes, effect that was more evident at the lowest temperatures. two kinetic inactivation models were used to f ... | 2012 | 22633800 |
| influence of flow direction and flow rate on the initial adhesion of seven listeria monocytogenes strains to fine polished stainless steel. | the effects of flow direction and shear stress on the adhesion of different strains of listeria monocytogenes to fine polished stainless steel under liquid flow conditions were investigated. furthermore, the relationship between cell surface properties and cell size and the initial adhesion rate (iar) was studied. a method, including fluorescence microscopy and a flow perfusion system, was developed and used to examine the real-time initial cell adhesion of different l. monocytogenes species in ... | 2012 | 22633798 |
| listeria monocytogenes: no spreading without no. | in infectious disease immunology, inducible nitric oxide (no) synthase-derived no is believed to function primarily as an antimicrobial and immunoregulatory molecule. in this issue of immunity, cole et al. (2012) show that no helps the intracellular bacteria listeria monocytogenes to spread. | 2012 | 22633455 |
| lack of pparγ in myeloid cells confers resistance to listeria monocytogenes infection. | the peroxisomal proliferator-activated receptor γ (pparγ) is a nuclear receptor that controls inflammation and immunity. innate immune defense against bacterial infection appears to be compromised by pparγ. the relevance of pparγ in myeloid cells, that organize anti-bacterial immunity, for the outcome of immune responses against intracellular bacteria such as listeria monocytogenes in vivo is unknown. we found that listeria monocytogenes infection of macrophages rapidly led to increased expressi ... | 2012 | 22629382 |
| listeria infection in patients on anti-tnf treatment: report of two cases and review of the literature. | listeria monocytogenes is an aerobic gram positive intracellular bacillus, predominantly affecting pregnant women, immunocompromised patients and old individuals. invasive listeriosis, meningitis and meningoencephalitis, bacteraemia with or without joint, eye or heart focalization are clinical manifestations of the disease. anti-tnf-α drugs blocking the host's response against various microorganisms, particularly intracellular agents like listeria monocytogenes, increase the risk of disease. we ... | 2013 | 22626505 |
| listeria monocytogenes sepsis in ulcerative colitis. | | 2012 | 22622754 |
| cellular pharmacokinetics and intracellular activity against listeria monocytogenes and staphylococcus aureus of chemically modified and nanoencapsulated gentamicin. | the aim of this study was to investigate different hydrophobic gentamicin formulations [gentamicin-bis(2-ethylhexyl) sulfosuccinate (gen-aot), microstructured gen-aot (pca gen-aot) and gen-aot-loaded poly(lactide-co-glycolide) acid (plga) nanoparticles (nps)] in view of improving its therapeutic index against intracellular bacteria. the intracellular accumulation, subcellular distribution and intracellular activity of gen-aot and nps in different monocytic-macrophagic cell lines were studied. | 2012 | 22615297 |
| listeria monocytogenes growth dynamics on produce: a review of the available data for predictive modeling. | several listeriosis outbreaks have been linked to the consumption of fresh or processed produce in recent years. one major determinant of the listeriosis risk is the ability of a food to support growth of listeria monocytogenes during storage. however, data regarding the ability to support growth of l. monocytogenes are scarce or nonexisting for many produce commodities. here we synthesize the available data regarding growth behavior of l. monocytogenes on produce, compare the growth data with l ... | 2012 | 22612229 |
| notch-rbp-j signaling regulates the transcription factor irf8 to promote inflammatory macrophage polarization. | emerging concepts suggest that the functional phenotype of macrophages is regulated by transcription factors that define alternative activation states. we found that rbp-j, the main nuclear transducer of signaling via notch receptors, augmented toll-like receptor 4 (tlr4)-induced expression of key mediators of classically activated m1 macrophages and thus of innate immune responses to listeria monocytogenes. notch-rbp-j signaling controlled expression of the transcription factor irf8 that induce ... | 2012 | 22610140 |
| screening and in situ synthesis using crystals of a nad kinase lead to a potent antistaphylococcal compound. | making new ligands for a given protein by in situ ligation of building blocks (or fragments) is an attractive method. however, it suffers from inherent limitations, such as the limited number of available chemical reactions and the low information content of usual chemical library deconvolution. here, we describe a focused screening of adenosine derivatives using x-ray crystallography. we discovered an unexpected and biocompatible chemical reactivity and have simultaneously identified the mode o ... | 2012 | 22608967 |
| plant-derived antimicrobials reduce listeria monocytogenes virulence factors in vitro, and down-regulate expression of virulence genes. | listeria monocytogenes (lm) is a major foodborne pathogen causing septicemia, meningitis and death in humans. lm infection is preceded by its attachment to and invasion of human intestinal epithelium followed by systemic spread. the major virulence factors in lm include motility, hemolysin and lecithinase production. reducing lm attachment to and invasion of host tissue and production of virulence factors could potentially control listeriosis in humans. this study investigated the efficacy of su ... | 2012 | 22608657 |
| prevalence, characterization and sources of listeria monocytogenes in blue crab (callinectus sapidus) meat and blue crab processing plants. | seven blue crab processing plants were sampled to determine the prevalence and sources of listeria spp. and listeria monocytogenes for two years (2006-2007). a total of 488 raw crabs, 624 cooked crab meat (crab meat) and 624 environmental samples were tested by standard methods. presumptive listeria spp. were isolated from 19.5% of raw crabs, 10.8% of crab meat, and 69.5% of environmental samples. l. monocytogenes was isolated from 4.5% of raw crabs, 0.2% of crab meat, and 2.1% of environmental ... | 2012 | 22608232 |
| survey of raw milk cheeses for microbiological quality and prevalence of foodborne pathogens. | cheese may be manufactured in the united states using raw milk, provided the cheese is aged for at least 60 days at temperatures not less than 35°f (1.7°c). there is now increased concern among regulators regarding the safety of raw milk cheese due to the potential ability of foodborne pathogens to survive the manufacturing and aging processes. in this study, 41 raw milk cheeses were obtained from retail specialty shops, farmers' markets, and on-line sources. the cheeses were then analyzed for t ... | 2012 | 22608218 |
| bayesian inference for quantifying listeria monocytogenes prevalence and concentration in minced pork meat from presence/absence microbiological testing. | the purpose of this work was to estimate the prevalence and concentration of listeria monocytogenes in minced pork meat by the application of a bayesian modeling approach. samples (n = 100) collected from local markets were tested for l. monocytogenes using in parallel the palcam, aloa and rapid'l.mono selective media. presence of the pathogen was confirmed through biochemical and molecular tests. independent experiments (n = 10) for validation purposes were performed. no l. monocytogenes was en ... | 2012 | 22608217 |
| clinical development of listeria monocytogenes-based immunotherapies. | active immunotherapy targeting dendritic cells (dcs) has shown great promise in preclinical models and in human clinical trials for the treatment of malignant disease. sipuleucel-t (provenge, dendreon, seattle, wa), which consists of antigen-loaded dendritic cells (dcs), recently became the first targeted therapeutic cancer vaccine to be approved by the us food and drug administration (fda). however, ex vivo therapies such as provenge have practical limitations and elicit an immune response with ... | 2012 | 22595054 |
| invasive listeriosis in the foodborne diseases active surveillance network (foodnet), 2004-2009: further targeted prevention needed for higher-risk groups. | listeriosis can cause severe disease, especially in fetuses, neonates, older adults, and persons with certain immunocompromising and chronic conditions. we summarize us population-based surveillance data for invasive listeriosis from 2004 through 2009. | 2012 | 22572660 |
| essential oil of the leaves of eugenia uniflora l.: antioxidant and antimicrobial properties. | essential oil (eo) of the leaves of eugenia uniflora l. (brazilian cherry tree) was evaluated for its antioxidant, antibacterial and antifungal properties. the acute toxicity of the eo administered by oral route was also evaluated in mice. the eo exhibited antioxidant activity in the dpph, abts and frap assays and reduced lipid peroxidation in the kidney of mice. the eo also showed antimicrobial activity against two important pathogenic bacteria, staphylococcus aureus and listeria monocytogenes, ... | 2012 | 22583648 |
| ciap1 and ciap2 limit macrophage necroptosis by inhibiting rip1 and rip3 activation. | cellular inhibitor of apoptosis proteins (ciaps) have emerged as important anti-cell death mediators, particularly in cancer. although they are known to be expressed in immune tissue, their specific immune function remains unclear. we observed that degradation of ciaps with smac mimetic (sm) results in death of primary bone-marrow-derived macrophages. sm-induced death of macrophages occurred by programmed necrosis (necroptosis), which was dependent on tnf receptor expression. consistent with nec ... | 2012 | 22576661 |
| precise detection of l. monocytogenes hitting its highly conserved region possessing several specific antibody binding sites. | listeria monocytogenes, a facultative intracellular fast-growing gram-positive food-borne pathogen, can infect immunocompromised individuals leading to meningitis, meningoencephalitis and septicaemias. from the pool of virulence factors of the organism, acta, a membrane protein, has a critical role in the life cycle of l. monocytogenes. high mortality rate of listeriosis necessitates a sensitive and rapid diagnostic test for precise identification of l. monocytogenes. we used bioinformatic tools ... | 2012 | 22575546 |
| fusion of the fc part of human igg1 to cd14 enhances its binding to gram-negative bacteria and mediates phagocytosis by fc receptors of neutrophils. | microbial resistance to antimicrobial drugs is promoting a search for new antimicrobial agents that target highly conservative structures of pathogens. human cd14 - a known pattern recognition receptor (prr) which recognizes multiple ligands from different microbes might be a worthy candidate. the aim of our work was to create a cd14/fc dimer protein and evaluate its whole bacteria binding and opsonizing capabilities. fusion of cd14 with the fragment crystallisable (fc) part of human igg1 could ... | 2012 | 22575527 |
| prevalence and antibiotic resistance of campylobacter, salmonella, and l. monocytogenes in ducks: a review. | campylobacter, salmonella, and listeria monocytogenes are important bacterial pathogens associated with gastroenteritis. the consumption of poultry meat and their products is considered as a major and leading source of human infection. while surveys of chicken meat and products, and its association with foodborne pathogens are widely available, such information on ducks is scarce. this survey examines the prevalence and antibiotic resistance of campylobacter, salmonella and l. monocytogenes isol ... | 2012 | 22571641 |
| effects of irradiation dose and o(2) and co(2) concentrations in packages on foodborne pathogenic bacteria and quality of ready-to-cook seasoned ground beef product (meatball) during refrigerated storage. | combined effects of gamma irradiation and concentrations of o(2) (0, 5, 21%) and co(2) (0, 50%) on survival of escherichia coli o157:h7, salmonella enteritidis, listeria monocytogenes, lipid oxidation, and color changes in ready-to-cook seasoned ground beef (meatball) during refrigerated storage were investigated. ground beef seasoned with mixed spices was packaged in varying o(2) and co(2) levels and irradiated at 2 and 4 kgy. irradiation (4 kgy) caused about 6 log inactivation of the inoculate ... | 2012 | 22566763 |
| global gene expression of listeria monocytogenes to salt stress. | outbreaks of listeriosis caused by the ingestion of listeria-contaminated ready-to-eat foods have been reported worldwide. many ready-to-eat foods, such as deli meat products, contain high amounts of salt, which can disrupt the maintenance of osmotic balance within bacterial cells. to understand how listeria monocytogenes adapts to salt stress, we examined the growth and global gene expression profiles of l. monocytogenes strain f2365 under salt stress using oligonucleotide probe-based dna array ... | 2012 | 22564940 |
| growth of listeria monocytogenes in different retail delicatessen meats during simulated home storage. | delicatessen meats are reported to be the leading vehicle of foodborne listeriosis in the united states. listeria monocytogenes can reach high numbers in these products during storage, and the growth rate is largely dictated by product formulation and storage temperature. to assess the impact of product age on listeria growth, five commercial brands each of cured and uncured turkey breast, ham, and roast beef (three lots per brand) were sliced (approximately 25 g per slice) at the beginning of t ... | 2012 | 22564939 |
| prevalence and distribution of listeria monocytogenes serotypes and pulsotypes in sows and fattening pigs in farrow-to-finish farms (france, 2008). | this study was undertaken to acquire new data on the prevalence of listeria monocytogenes in sows and fattening pigs in farrow-to-finish pig farms, and to analyze distribution of serotypes and genotypes of the bacterium within farms. detection of l. monocytogenes was carried out on 730 pooled feces samples from sows in 73 pig farms and on 172 pooled feces samples from fattening pigs in 43 of these farms. isolates were serotyped and typed by pulsed-field gel electrophoresis. for sows, 46% of the ... | 2012 | 22564938 |
| genes encoding putative dead-box rna helicases in listeria monocytogenes egd-e are needed for growth and motility at 3°c. | quantitative rt-pcr revealed that transcripts of all four putative dead-box rna helicase genes of the psychrotrophic pathogen listeria monocytogenes egd-e are found at higher levels in organisms grown at 3°c than at 37°c. at 3°c, growth of the three corresponding gene deletion mutants δlmo0866, δlmo1450 and δlmo1722 was clearly restricted. the minimum growth temperatures of the three mutants were also higher than that of the wild-type egd-e. in addition to inability to grow at 3°c, growth of δlm ... | 2012 | 22564273 |
| antimicrobial activities of therapeutic herbal plants against listeria monocytogenes and the herbal plant cytotoxicity on caco-2 cell. | this study investigated the antimicrobial effect of various therapeutic herbal plants on listeria monocytogenes, and their cytotoxicity effect on mammalian cells. | 2012 | 22563627 |
| inhibition of calpain blocks the phagosomal escape of listeria monocytogenes. | listeria monocytogenes is a gram-positive facultative intracellular bacterium responsible for the food borne infection listeriosis, affecting principally the immunocompromised, the old, neonates and pregnant women. following invasion l. monocytogenes escapes the phagosome and replicates in the cytoplasm. phagosome escape is central to l. monocytogenes virulence and is required for initiating innate host-defence responses such as the secretion of the cytokine interleukin-1. phagosome escape of l. ... | 2012 | 22563421 |
| genetic organization of ascb-dape internalin cluster serves as a potential marker for listeria monocytogenes sublineages iia, iib, and iic. | listeria monocytogenes is an important foodborne pathogen that comprises four genetic lineages: i, ii, iii, and iv. of these, lineage ii is frequently recovered from foods and environments and responsible for the increasing incidence of human listeriosis. in this study, the phylogenetic structure of lineage ii was determined through sequencing analysis of the ascb-dape internalin cluster. fifteen sequence types proposed by multilocus sequence typing based on nine housekeeping genes were grouped ... | 2012 | 22561849 |
| growth potential of salmonella spp. and listeria monocytogenes in nine types of ready-to-eat vegetables stored at variable temperature conditions during shelf-life. | growth potential (δ) is defined as the difference between the population of a microorganism at the end of shelf-life of specific food and its initial population. the determination of δ of salmonella and listeria monocytogenes in rte vegetables can be very useful to determine likely threats to food safety. however, little is known on the behavior of these microorganisms in several rte vegetables. therefore, the aim of this study was to determine the δ of both pathogens in nine different types of ... | 2012 | 22561064 |
| adaptor protein-3 in dendritic cells facilitates phagosomal toll-like receptor signaling and antigen presentation to cd4(+) t cells. | effective major histocompatibility complex-ii (mhc-ii) antigen presentation from phagocytosed particles requires phagosome-intrinsic toll-like receptor (tlr) signaling, but the molecular mechanisms underlying tlr delivery to phagosomes and how signaling regulates antigen presentation are incompletely understood. we show a requirement in dendritic cells (dcs) for adaptor protein-3 (ap-3) in efficient tlr recruitment to phagosomes and mhc-ii presentation of antigens internalized by phagocytosis bu ... | 2012 | 22560444 |
| effects of x-ray treatments on pathogenic bacteria, inherent microflora, color, and firmness on whole cantaloupe. | inactivation of inoculated escherichia coli o157:h7, listeria monocytogenes, salmonella enterica and shigella flexneri on whole cantaloupes using x-ray at different doses (0.1, 0.5, 1.0, 1.5, and 2.0 kgy) was studied. the effect of x-ray on quality parameters (color and texture) of untreated and treated whole cantaloupes was instrumentally determined. the effect of x-ray on microflora counts (mesophilic counts, psychrotrophic counts and yeast and mold counts) of untreated and treated whole canta ... | 2012 | 22560020 |
| risk-based sampling plan to control listeria monocytogenes in a hospital food service operation. | | 2012 | 22558755 |
| effect of liposome-encapsulated nisin and bacteriocin-like substance p34 on listeria monocytogenes growth in minas frescal cheese. | the efficacy of liposome-encapsulated nisin and bacteriocin-like substance (bls) p34 to control growth of listeria monocytogenes in minas frescal cheese was investigated. nisin and bls p34 were encapsulated in partially purified soybean phosphatidylcholine (pc-1) and pc-1-cholesterol (7:3) liposomes. pc-1 nanovesicles were previously characterized. pc-1-cholesterol encapsulated nisin and bls p34 presented, respectively, 218 nm and 158 nm diameters, zeta potential of -64 mv and -53 mv, and entrap ... | 2012 | 22554928 |
| prevalence and risk factors for listeria monocytogenes in broiler flocks in shiraz, southern iran. | listeria monocytogenes has been identified as an important foodborne pathogen in recent years. in humans, it most commonly affects pregnant women, neonates, children, elderly people, and persons with a suppressed immune system. it could contaminate both raw and cooked meat and poultry products. studies regarding prevalence and risk factors of l. monocytogenes in broilers flocks are limited. therefore, the present study was conducted to determine the prevalence and risk factors for l. monocytogen ... | 2012 | 22551072 |
| sanitation of selected ready-to-eat intermediate-moisture foods of animal origin by e-beam irradiation. | to optimize the sanitation treatment of ready-to-eat (rte) intermediate-moisture foods (imf), the behavior of listeria monocytogenes scott a (cip 103575), l. innocua (ntc 11288), salmonella enterica serovar typhimurium (cect 443), and escherichia coli o157:h7 (cect 4972) following treatment with electron-beam irradiation has been studied. as food matrixes, three rte vacuum-packed products (iberian dry-cured ham, dry beef [cecina], and smoked tuna) were used. although an irradiation treatment is ... | 2012 | 22551071 |
| listeria weihenstephanensis sp. nov., isolated from the water plant lemna trisulca taken from a freshwater pond. | the phylogenetic position and phenotypic characteristics of two non-spore-forming bacilli similar to members of the genus listeria were studied. the gram-reaction-positive, slightly motile, facultatively anaerobic strains were isolated from the water plant lemna trisulca sampled from a freshwater pond in bavaria, germany. although no identification was possible employing the api listeria test (biomérieux), 16s rrna sequence analysis confirmed a close phylogenetic similarity to listeria rocourtia ... | 2013 | 22544790 |
| [listeria monocytogenes and herpes simplex type 1, associated in a meningoencephalitis episode in an immunodepressed patient]. | | 2012 | 22542878 |
| development and optimization of an egfp-based reporter for measuring the general stress response in listeria monocytogenes. | a characteristic of the food-borne pathogen listeria monocytogenes is its tolerance to the harsh conditions found both in minimally processed foods and the human gastrointestinal tract. this trait is partly under the control of the alternative sigma factor sigma b (σ(b)). to study the mechanisms that trigger the activation of σ(b) , and hence the development of stress tolerance, we have developed a fluorescent reporter fusion that allows the real-time activity of σ(b) to be monitored. the report ... | 2015 | 22539028 |
| [liver abscess due to listeria monocytogenes]. | | 2013 | 22537897 |
| comparative spatiotemporal analysis of the intrathecal immune response in natural listeric rhombencephalitis of cattle and small ruminants. | this study examined the spatiotemporal immune response in listeric rhombencephalitis of ruminants in situ. our data support the view that astrocytes facilitate the containment of infectious lesions. results on the natural disease recapitulate observations in experimental rodent models and suggest that the mounted adaptive lymphocytic response of ruminants is effective in eliminating listeria monocytogenes (lm). however, our data indicate earlier participation of the adaptive immune response, a s ... | 2012 | 22537479 |
| single-cell growth probability of listeria monocytogenes at suboptimal temperature, ph, and water activity. | the single-cell growth probability of listeria monocytogenes was characterized in tryptone soy broth supplemented with 0.6% yeast extract at temperature ranging from 5 to 25°c, ph ranging from 4.4 to 6.5, and water activity ranging from 0.919 to 0.989. growth was monitored visually in 96-microwell plates and the growth probability was deduced from concentrations estimated with the mpn calculation. models were proposed to describe the increase of the probability from 0 to 1 with increasing values ... | 2012 | 22536203 |
| clinical, pathological, and genetic characterization of listeria monocytogenes causing sepsis and necrotizing typhlocolitis and hepatitis in a foal. | listeria monocytogenes was isolated from the blood, lungs, and liver of a 5-week-old american quarter horse filly that presented with a 2-day history of fever, lethargy, ataxia, and seizure activity. the foal was born on a well-managed breeding facility to a multiparous mare with no periparturient complications. at 8 hr of age, the foal had an adequate passive transfer of immunity (immunoglobulin g > 2,000 mg/dl). since the time of birth, the foal reportedly had mild, intermittent diarrhea that ... | 2012 | 22529130 |
| carbon catabolite control is important for listeria monocytogenes biofilm formation in response to nutrient availability. | the foodborne pathogen listeria monocytogenes has the ability to develop biofilm in food-processing environment, which becomes a major concern for the food safety. the biofilm formation is strongly influenced by the availability of nutrients and environmental conditions, and particularly enhanced in poor minimal essential medium (mem) containing glucose rather than in rich brain heart infusion (bhi) broth. to gain better insight into the conserved protein expression profile in these biofilms, th ... | 2012 | 22526570 |
| listeria fleischmannii sp. nov., isolated from cheese. | a study was performed on three isolates (lu2006-1(t), lu2006-2 and lu2006-3), which were sampled independently from cheese in western switzerland in 2006, as well as a fourth isolate (a11-3426), which was detected in 2011, using a polyphasic approach. the isolates could all be assigned to the genus listeria but not to any known species. phenotypic and chemotaxonomic data were compatible with the genus listeria and phylogenetic analysis based on 16s rrna gene sequences confirmed that the closest ... | 2013 | 22523164 |
| re-evaluation of the significance of penicillin binding protein 3 in the susceptibility of listeria monocytogenes to β-lactam antibiotics. | penicillin binding protein 3 (pbp3) of l. monocytogenes has long been thought of as the primary lethal target for β-lactam antibiotics due to the excellent correlation between the mics of different β-lactams and their affinity for this protein. the gene encoding pbp3 has not yet been directly identified in this gram-positive bacterium, but based on in silico analysis, this protein is likely to be encoded by lmo1438. however, studies examining the effects of mutations in genes encoding known and ... | 2012 | 22513233 |
| [microbiological characterisation of listeria monocytogenes isolates from human cases in andalusia]. | the aim of this study was to perform a retrospective study by genotyping 154 isolates from human listeriosis cases occurred in the region of andalusia (southern spain) in the period 2005-2009. | 2012 | 22513092 |
| listeria monocytogenes strain-specific impairment of the tetr regulator underlies the drastic increase in cyclic di-amp secretion and beta interferon-inducing ability. | among a number of laboratory strains of listeria monocytogenes used in experimental infection, strain lo28 is highly capable of inducing robust beta interferon (ifn-β) production in infected macrophages. in this study, we investigated the molecular mechanism of the ifn-β-inducing ability of lo28 by comparing it with that of strain egd, a low-ifn-β-inducing strain. it was found that lo28 secretes a large amount of ifn-β-inducing factor, which turned out to be cyclic di-amp. the secretion of cycli ... | 2012 | 22508860 |
| estimating the diagnostic accuracy of three culture-dependent methods for the listeria monocytogenes detection from a bayesian perspective. | the objective of this study was to estimate the test accuracy measures (classification probabilities [cps], predictive values [pvs], likelihood ratios [lrs] and area under receiving operating characteristic curve [auc]) of three different culture-dependent methods, commonly used during routine analysis for the detection of the foodborne pathogen listeria monocytogenes, from a bayesian perspective. data from a previous study by andritsos et al. (2010) were used to define measures of accuracy for ... | 2012 | 22507629 |
| purine biosynthesis mutants (pura and purb) of serotype 4b listeria monocytogenes are severely attenuated for systemic infection in intragastrically inoculated a/j mice. | in this study, we demonstrate that pura and purb transposon mutants of serotype 4b listeria monocytogenes were severely impaired in their ability to colonize the gastrointestinal tract and cause systemic infection of the spleen, liver, and gallbladder following intragastric inoculation of a/j mice. the mutant strains were also impaired in their ability to multiply within caco-2 human intestinal epithelial cells. neither mutant was affected in resistance to synthetic gastric fluid (ph 4.5). these ... | 2012 | 22506772 |
| purification, crystallization and preliminary crystallographic studies of a pacl homologue from listeria monocytogenes. | ca(2+)-atpases are members of a large family of membrane proteins that maintain the selective movement of cations across biological membranes. a putative listeria monocytogenes ca(2+)-atpase (lmo0818) was crystallized in an unknown functional state. the crystal belonged to space group p2(1)2(1)2(1) and a complete data set was collected to 3.2 å resolution. the molecular-replacement solution obtained revealed that lmo0818 is likely to adopt an e2-like state mimicking the phosphorylated intermedia ... | 2012 | 22505411 |
| structural analysis of a 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase with an n-terminal chorismate mutase-like regulatory domain. | 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (dahps) catalyzes the first step in the biosynthesis of a number of aromatic metabolites. likely because this reaction is situated at a pivotal biosynthetic gateway, several dahps classes distinguished by distinct mechanisms of allosteric regulation have independently evolved. one class of dahpss contains a regulatory domain with sequence homology to chorismate mutase-an enzyme further downstream of dahps that catalyzes the first committed ste ... | 2012 | 22505283 |
| hmgr, a key enzyme in the mevalonate pathway for isoprenoid biosynthesis, is essential for growth of listeria monocytogenes egde. | isoprenoids may be synthesized via one of two pathways, the classical mevalonate pathway or the alternative 2-c-methyl-d-erythritol 4-phosphate (mep) pathway. while the majority of bacteria utilize a single pathway for isoprenoid biosynthesis, listeria monocytogenes is unusual in possessing the complete set of genes for both pathways. here, we utilized new molecular tools to create precise gene deletions in selected genes encoding enzymes of both pathways, gcpe, lytb (encoding proteins in the me ... | 2012 | 22504435 |
| effect of anolyte on background microflora, salmonella, and listeria monocytogenes on catfish fillets. | near-neutral electrolyzed water (anolyte), having a ph of 6.0 to 6.5 ± 0.02, oxidation reduction potential of greater than 700 mv, and a residual chlorine level of 10 to 200 ppm, was reported to have a potential use to decontaminate food surfaces. an electrolyzing cell was developed that is capable of producing neutral electrolyzed water containing a chlorine level of greater than 700 ppm in the form of hypochlorous acid (anolyte). anolyte with a chlorine level of 300 ppm was used to determine i ... | 2012 | 22488069 |
| heat shock and cold shock treatments affect the survival of listeria monocytogenes and salmonella typhimurium exposed to disinfectants. | the foodborne pathogens listeria monocytogenes and salmonella typhimurium were subjected to heat shock at 48°c for 10 and 30 min, respectively, and then cold shocked at 15°c for 3 h. the effect of these shocks on the viability of test organisms exposed to chlorine dioxide and quaternary ammonium compounds was then determined. after exposure to the disinfectants, the viable population of each test organism, regardless of heat shock or cold shock treatment, decreased as the exposure period was ext ... | 2012 | 22488057 |
| lm-llo-based immunotherapies and hpv-associated disease. | hpv infection is a direct cause of neoplasia and malignancy. cellular immunologic activity against cells expressing hpv e6 and e7 is sufficient to eliminate the presence of dysplastic or neoplastic tissue driven by hpv infection. live attenuated listeria monocytogenes- (lm-) based immunotherapy (adxs11-001) has been developed for the treatment of hpv-associated diseases. adxs11-001 secretes an antigen-adjuvant fusion (lm-llo) protein consisting of a truncated fragment of the lm protein listeriol ... | 2012 | 22481930 |
| effects of nisin, edta and salts of organic acids on listeria monocytogenes, salmonella and native microflora on fresh vacuum packaged shrimps stored at 4 °c. | nisin (500 iu ml⁻¹), edta (0.02 m), potassium sorbate (ps) (3%, w/v), sodium benzoate (sb) (3%, w/v) or sodium diacetate (sd) (3%, w/v); alone or in combination were used to dip uninoculated shrimps and shrimps inoculated with listeria monocytogenes or salmonella (∼4.0-5.0 log cfu g⁻¹). shrimps were then drip-dried, vacuum packaged and stored at 4 °c for 7 days. untreated shrimps were used as a control. numbers of l. monocytogenes, salmonella and native background microflora were determined on u ... | 2012 | 22475941 |
| photodynamic inactivation of methylene blue and tungsten-halogen lamp light against food pathogen listeria monocytogenes. | the aim of this study was to verify the bactericidal effect and the damage of photodynamic inactivation (pdi) using methylene blue (mb) and tungsten-halogen lamp over listeria monocytogenes via atomic force microscopy, absorption spectrophotometry, agarose gel electrophoresis, real-time pcr and sds-page. the obtained data indicated that the viability of l. monocytogenes was ca 7-log reduced by illumination with 10 min tungsten-halogen lamp light under the presence of 0.5 μg ml(-1) mb, and this b ... | 2013 | 22469298 |
| effective inactivation of food pathogens listeria monocytogenes and salmonella enterica by combined treatment of hypericin-based photosensitization and high power pulsed light. | the aim of this study was to evaluate the inactivation efficiency of listeria monocytogenes atc(l3) c 7644 and salmonella enterica serovar typhimurium strain ds88 by combined treatment of hypericin (hyp)-based photosensitization and high power pulsed light (hppl). | 2012 | 22469030 |
| biotecon diagnostics foodproof listeria monocytogenes detection kit, 5' nuclease in combination with the foodproof shortprep ii kit. | a method was developed for the detection of listeria monocytogenes in food. the method is based on real-time pcr using hydrolysis probes (5' nuclease). this advanced pcr method was designed to reduce the time necessary to achieve results from pcr reactions and to enable the user to monitor the amplification of the pcr product simultaneously, in real-time. after dna isolation using the biotecon foodproof shortprep ii kit designed for the rapid preparation of l. monocytogenes dna for direct use in ... | 2012 | 22468346 |
| efficacy of octenidine hydrochloride for reducing escherichia coli o157:h7, salmonella spp., and listeria monocytogenes on cattle hides. | the efficacy of octenidine hydrochloride (oh; 0.025, 0.15, and 0.25%) for inactivating escherichia coli o157:h7, salmonella spp., and listeria monocytogenes on cattle hides was investigated at 23°c in the presence and absence of bovine feces. all tested concentrations of oh were effective in decreasing more than 5.0 log cfu of bacteria/cm(2) in 5 min (p < 0.01). the results suggest that oh could be used to decontaminate cattle hides; however, further studies under commercial settings are necessa ... | 2012 | 22467506 |
| validation of direct plating of a stool sample as a method for listeria monocytogenes detection. | the aim of current studies was to validate the direct plating of a stool sample for listeria monocytogenes detection, using selective medium palcam agar with palcam selective supplement. validation was performed using stool samples collected from healthy humans inoculated with listeria sp. strains. stool samples were frozen to determine the influence of freezing on method robustness. the presented research defines the listeria monocytogenes limit of detection (lod) as 10(3) cfu/g of stools for f ... | 2012 | 22462448 |
| pcr-based screening of targeted mutants for the fast and simultaneous identification of bacterial virulence factors. | understanding the strategies used by pathogens to infect, survive, and proliferate in their hosts requires the identification of virulence factors. we developed pcr-based screening of targeted mutants to facilitate quick, simultaneous detection of multiple novel bacterial virulence genes. based on direct pcr screening of pooled targeted mutants, this approach provides a fast and sensitive measure of virulence attenuation while significantly reducing the number of animals and time required. we de ... | 2012 | 26307257 |
| yogi berra, forrest gump, and the discovery of listeria actin comet tails. | in 1988, eminent cell biologist lew tilney and newly appointed assistant professor of microbiology dan portnoy met at a picnic and initiated a collaboration that led to a groundbreaking paper published in journal of cell biology entitled "actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, listeria monocytogenes." the paper has been cited more than 800 times, the most of any publication in the careers of both investigators. using an electron microscope f ... | 2012 | 22461646 |
| high density microarray analysis reveals new insights into genetic footprints of listeria monocytogenes strains involved in listeriosis outbreaks. | listeria monocytogenes, a foodborne bacterial pathogen, causes invasive and febrile gastroenteritis forms of listeriosis in humans. both invasive and febrile gastroenteritis listeriosis is caused mostly by serotypes 1/2a, 1/2b and 4b strains. the outbreak strains of serotype 1/2a and 4b could be further classified into several epidemic clones but the genetic bases for the diverse pathophysiology have been unsuccessful. dna microarray provides an important tool to scan the entire genome for genet ... | 2012 | 22457724 |
| a comparison of methods used to extract bacterial dna from raw milk and raw milk cheese. | in this study, we compare seven different methods which have been designed or modified to extract total dna from raw milk and raw milk cheese with a view to its subsequent use for the pcr of bacterial dna. | 2012 | 22452460 |
| development of a single-gene, signature-tag-based approach in combination with alanine mutagenesis to identify listeriolysin o residues critical for the in vivo survival of listeria monocytogenes. | listeriolysin o (llo) is a pore-forming toxin of the cholesterol-dependent cytolysin (cdc) family and a primary virulence factor of the intracellular pathogen listeria monocytogenes. llo mediates rupture of phagosomal membranes, thereby releasing bacteria into the growth-permissive host cell cytosol. several unique features of llo allow its activity to be precisely regulated in order to facilitate phagosomal escape, intracellular growth, and cell-to-cell spread. to improve our understanding of t ... | 2012 | 22451517 |
| listeria monocytogenes: generalised maculopapular rash may be the clue. | | 2013 | 22447990 |
| listeria monocytogenes grown at 7° c shows reduced acid survival and an altered transcriptional response to acid shock compared to l. monocytogenes grown at 37° c. | survival of the food-borne pathogen listeria monocytogenes in acidic environments (e.g., in the human stomach) is vital to its transmission. refrigerated, ready-to-eat foods have been sources of listeriosis outbreaks. the purpose of this study was to determine whether growth at a low temperature (i.e., 7°c) affects l. monocytogenes survival or gene transcription after exposure to a simulated gastric environment (i.e., acid shock at 37°c). l. monocytogenes cells grown at 7°c were less resistant t ... | 2012 | 22447604 |
| reduction in listeria monocytogenes and spoilage bacteria on smoked catfish using x-ray treatments. | to determine the efficacy of x-ray processes in inactivating l. monocytogenes levels in smoked catfish during storage at 5°c and to determine the effects of x-ray doses on controlling the growth of spoilage bacteria on smoked catfish during storage at 5°c for up to 5 weeks. | 2012 | 22429153 |
| linkage of bacterial protein synthesis and presentation of mhc class i-restricted listeria monocytogenes-derived antigenic peptides. | the processing and mhc class i-restricted presentation of antigenic peptides derived from the p60 protein of the facultative intracellular bacterium listeria monocytogenes is tightly linked to bacterial protein synthesis. we used non-linear regression analysis to fit a mathematical model of bacterial antigen processing to a published experimental data set showing the accumulation and decay of p60-derived antigenic peptides in l. monocytogenes-infected cells. two alternative models equally descri ... | 2012 | 22428021 |
| capillary electrophoresis separation of protein composition of γ-irradiated food pathogens listeria monocytogenes and staphylococcus aureus. | a capillary electrophoresis method using uv detection was developed to analyse protein composition of the lysates of two foodborne pathogens, listeria monocytogenes and staphylococcus aureus which were previously treated at different irradiation doses. | 2012 | 22427846 |
| inhibition of listeria monocytogenes on bologna sausages by an antimicrobial film containing mustard extract or sinigrin. | the ability of listeria (l.) monocytogenes to convert glucosinolates into antimicrobial isothiocyanates was investigated. mustard glucosinolates in pure (sinigrin) or extract forms (sinigrin, oriental; sinalbin, yellow mustard) were used in broth media and in a polyvinyl polyethylene glycol graft copolymer (ppg) packaging film with bologna to examine their value as antimicrobial precursors for the control of l. monocytogenes viability and extension of bologna shelf-life at 4 °c. during broth tes ... | 2012 | 22424932 |
| shigella flexneri utilize the spectrin cytoskeleton during invasion and comet tail generation. | the spectrin cytoskeleton is emerging as an important host cell target of enteric bacterial pathogens. recent studies have identified a crucial role for spectrin and its associated proteins during key pathogenic processes of listeria monocytogenes and salmonella typhimurium infections. here we investigate the involvement of spectrin cytoskeletal components during the pathogenesis of the invasive pathogen shigella flexneri. | 2012 | 22424399 |
| probabilistic models for the prediction of target growth interfaces of listeria monocytogenes on ham and turkey breast products. | this study developed growth/no growth models for predicting growth boundaries of listeria monocytogenes on ready-to-eat cured ham and uncured turkey breast slices as a function of lactic acid concentration (0% to 4%), dipping time (0 to 4 min), and storage temperature (4 to 10 °c). a 10-strain composite of l. monocytogenes was inoculated (2 to 3 log cfu/cm²) on slices, followed by dipping into lactic acid and storage in vacuum packages for up to 30 d. total bacterial (tryptic soy agar plus 0.6% ... | 2011 | 22417516 |
| rapid detection of listeria monocytogenes in raw milk with loop-mediated isothermal amplification and chemosensor. | loop-mediated isothermal amplification (lamp) allows a rapid amplification of nucleic acids under isothermal conditions. it can be combined with a rhodamine-based dual chemosensor for much more efficient, field-friendly detection of listeria monocytogenes. in this report, lamp was performed at 63 °c for 10 min, followed by a rapid reaction of dna amplification and the byproduct, pyrophosphate ion, with a rhodamine-based dual chemosensor and cu(2+) is visualized as a disappearance of red color. t ... | 2014 | 22416713 |
| inhibition of listeria monocytogenes by exposure to a combination of nisin and cold-pressed terpeneless valencia oil. | listeria monocytogenes is an important foodborne pathogen and its control in foods is a significant challenge. this study evaluated the effectiveness of nisin and cold-pressed terpeneless valencia oil (cptvo) on limiting l. monocytogenes growth. disk diffusion assays were performed to determine the effects of cptvo and nisin individually and in combination. together, these antimicrobials produced a zone of inhibition that was significantly larger (p < 0.05) than zones correlating to cptvo or nis ... | 2012 | 22416711 |
| critical issues in detecting viable listeria monocytogenes cells by real-time reverse transcriptase pcr. | rapid and specific detection of viable listeria monocytogenes cells, particularly in processed foods, is a major challenge in the food industry. to assess the suitability of using rna-based detection methods to detect viable cells, several sets of pcr primers and florescent probes were designed targeting the 16s rrna, internalin a, and ribosomal protein l4 genes. one-step real-time reverse transcriptase (rt) pcr assays were conducted using rnas extracted from control and heat-treated l. monocyto ... | 2012 | 22410225 |
| monitoring transmission routes of listeria spp. in smoked salmon production with repetitive element sequence-based pcr techniques. | various techniques have been used for tracing the transmission routes of listeria species and for the assessment of hygiene standards in food processing plants. the potential of repetitive element sequence-based pcr (rep-pcr) methods (gtg₅ and repi + ii) for the typing of listeria isolates (n = 116), including listeria monocytogenes (n = 46), was evaluated in a particular situation arising from the relocation of a company producing cold-smoked salmon. pulsed-field gel electrophoresis (pfge) usin ... | 2012 | 22410224 |
| characterization of the intracellular glutamate decarboxylase system: analysis of its function, transcription, and role in the acid resistance of various strains of listeria monocytogenes. | the glutamate decarboxylase (gad) system is important for the acid resistance of listeria monocytogenes. we previously showed that under acidic conditions, glutamate (glt)/γ-aminobutyrate (gaba) antiport is impaired in minimal media but not in rich ones, like brain heart infusion. here we demonstrate that this behavior is more complex and it is subject to strain and medium variation. despite the impaired glt/gaba antiport, cells accumulate intracellular gaba (gaba(i)) as a standard response agai ... | 2012 | 22407692 |
| listeriolysin o is strongly immunogenic independently of its cytotoxic activity. | the presentation of microbial protein antigens by major histocompatibility complex (mhc) molecules is essential for the development of acquired immunity to infections. however, most biochemical studies of antigen processing and presentation deal with a few relatively inert non-microbial model antigens. the bacterial pore-forming toxin listeriolysin o (llo) is paradoxical in that it is cytotoxic at nanomolar concentrations as well as being the source of dominant cd4 and cd8 t cell epitopes follow ... | 2012 | 22403645 |
| listeria monocytogenes and diet during pregnancy; balancing nutrient intake adequacy v. adverse pregnancy outcomes. | to evaluate the impact of adherence to public health recommendations on listeria monocytogenes food safety to limit exposure to potential food sources on micronutrient intakes of pregnant women and whether more frequent consumption of 'high-risk' foods increases risk for adverse pregnancy outcomes. | 2012 | 22397868 |
| enhanced inhibition of listeria monocytogenes by a combination of cold pressed terpeneless valencia orange oil and antibiotics. | this study was designed to evaluate the ability of cold pressed terpeneless valencia orange oil (cptvo) to enhance the effectiveness of antibiotics against 10 strains of listeria monocytogenes. disc diffusion assays were performed to determine the effects of cptvo and two antibiotics with different mechanisms of action (i.e., penicillin and chloramphenicol) individually and in combination with cptvo. cptvo alone produced zones ranging from 16.5 to 19.9 mm. penicillin at 2 or 10 units produced zo ... | 2012 | 22394025 |
| extracellular superoxide dismutase inhibits innate immune responses and clearance of an intracellular bacterial infection. | reactive oxygen species and reactive nitrogen species play important roles during immune responses to bacterial pathogens. extracellular superoxide dismutase (ecsod) regulates extracellular concentrations of reactive oxygen species and reactive nitrogen species and contributes to tissue protection during inflammatory insults. the participation of ecsod in immune responses seems therefore intuitive, yet is poorly understood. in the current study, we used mice with varying levels of ecsod activity ... | 2012 | 22393157 |
| [listeria monocytogenes--occurrence in food]. | | 2011 | 22389934 |
| interaction of listeria monocytogenes autolysin amidase with glycosaminoglycans promotes listerial adhesion to mouse hepatocytes. | adherence to the cell surface is a key event during infection of pathogenic microorganisms. we have previously reported that autolysin amidase (ami) of listeria monocytogenes promotes an efficient listerial adherence to mouse hepatocytes and requires for listerial pathogenicity. cell wall anchoring (cwa) domain of ami has been shown to bind lipoteichoic acid on listerial cell wall but the binding of ami to host cell surface molecules remains to be determined. in this study, we present evidence h ... | 2012 | 22386869 |
| gold nanoparticles/horseradish peroxidase encapsulated polyelectrolyte nanocapsule for signal amplification in listeria monocytogenes detection. | bioconjugate nanocapsules were fabricated by using polystyrene sulfonate (pss) to encapsulate gold nanoparticles (aunps) bearing adsorbed horseradish peroxidase (hrp). the average size of nanocapsule was in a range 150-400 nm. the efficiency of the capsules to enhance signals in an immunoassay was demonstrated by using an enzyme linked immunosorbent assay (elisa) to detect the food-borne pathogen -listeria monocytogenes. the antibody adsorbed onto the pss shell of the nanocapsules provided the r ... | 2012 | 22386483 |
| the microbiological conditions of carcasses from large game animals in italy. | this study investigates the microbiological conditions of large game animal carcasses following evisceration. carcasses of animals (n=291) hunted in the upper susa valley (italian alps) were analysed for ph, aerobic viable count (avc), enterobacteriaceae, yersinia spp., listeria monocytogenes and salmonella spp. after shooting, evisceration occurred within 60 min in 90.7% of animals and sampling within 90 min in 88.3% of animals. mean ph values (5.97: ruminants; 5.77: wild boar) were similar to ... | 2012 | 22386324 |
| [comparison of growth models of listeria monocytogenes in chilled pork]. | to investigate the growth of listeria monocytogenes (lm) in chilled pork at four temperature and to evaluate the accuracy of three predictive models- gp (growth predictor), pmp (pathogen modeling programme) and cp (combase predictor) in predicting lm growth in chilled pork. | 2011 | 22379803 |
| detection of listeria monocytogenes in ready-to-eat food by step one real-time polymerase chain reaction. | the aim of this study was to follow contamination of ready-to-eat food with listeria monocytogenes by using the step one real time polymerase chain reaction (pcr). we used the prepseq rapid spin sample preparation kit for isolation of dna and microseq® listeria monocytogenes detection kit for the real-time pcr performance. in 30 samples of ready-to-eat milk and meat products without incubation we detected strains of listeria monocytogenes in five samples (swabs). internal positive control (ipc) ... | 2012 | 22375593 |
| investigation of the listeria monocytogenes scott a acid tolerance response and associated physiological and phenotypic features via whole proteome analysis. | the global proteomic responses of the foodborne pathogen listeria monocytogenes strain scott a, during active growth and transition to the stationary growth phase under progressively more acidic conditions, created by addition of lactic acid and hcl, were investigated using label-free liquid chromatography/tandem mass spectrometry. approximately 56% of the scott a proteome was quantitatively assessable, and the data provides insight into its acquired acid tolerance response (atr) as well as the ... | 2012 | 22372944 |
| sweet buttermilk intake reduces colonisation and translocation of listeria monocytogenes in rats by inhibiting mucosal pathogen adherence. | the bovine milk fat globule membrane (mfgm) contains several antimicrobial components with proven efficacy in vitro, but in vivo evidence is scarce. the present study was performed to determine the efficacy of the bovine mfgm in vivo. rats were fed diets based on bovine skimmed milk powder (low in mfgm) or bovine sweet buttermilk powder (high in mfgm). after dietary adaptation, rats were orally infected with salmonella enteritidis or listeria monocytogenes. whereas sweet buttermilk powder did no ... | 2012 | 22370235 |
| enhancement of protective immunity against intracellular bacteria using type-1 polarized dendritic cell (dc) vaccine. | the development of effective vaccine strategies for intracellular bacteria, including tuberculosis, is one of the major frontiers of medical research. our previous studies showed that dendritic cell (dc) vaccine is a promising approach for eliciting protective immunity against intracellular bacteria. however, it has been reported that standard fully mature dcs show reduced ability to produce il-12p70 upon subsequent interaction with antigen (ag)-specific t cells, limiting their in vivo performan ... | 2012 | 22365841 |
| studies on the pathogenesis and survival of different culture forms of listeria monocytogenes to pulsed uv-light irradiation after exposure to mild-food processing stresses. | the effects of mild conventional food-processing conditions on listeria monocytogenes survival to pulsed uv (puv) irradiation and virulence-associated characteristics were investigated. specifically, this study describes the inability of 10 strains representative of 3 different culture forms or morphotypes of l. monocytogenes to adapt to normally lethal levels of puv-irradiation after exposure to sub-lethal concentrations of salt (7.5% (w/v) nacl for 1 h), acid (ph 5.5 for 1 h), heating (48 °c f ... | 2012 | 22365345 |
| microbial pattern recognition causes distinct functional micro-rna signatures in primary human monocytes. | micro-rnas (mirnas) are short, non-coding rnas that regulate gene expression post transcriptionally. several studies have demonstrated the relevance of mirnas for a wide range of cellular mechanisms, however, the current knowledge on how mirnas respond to relevant external stimuli, e.g. in disease scenarios is very limited. to generate a descriptive picture of the mirna network associated to inflammatory responses, we quantified the levels of 330 mirnas upon stimulation with a panel of pro-infla ... | 2012 | 22363568 |
| a putative p-type atpase required for virulence and resistance to haem toxicity in listeria monocytogenes. | regulation of iron homeostasis in many pathogens is principally mediated by the ferric uptake regulator, fur. since acquisition of iron from the host is essential for the intracellular pathogen listeria monocytogenes, we predicted the existence of fur-regulated systems that support infection. we examined the contribution of nine fur-regulated loci to the pathogenicity of l. monocytogenes in a murine model of infection. while mutating the majority of the genes failed to affect virulence, three mu ... | 2012 | 22363518 |
| sale of raw milk in northern italy: food safety implications and comparison of different analytical methodologies for detection of foodborne pathogens. | the safety of raw milk sold in northern italy was investigated in relation to hygiene quality parameters and presence of salmonella spp., listeria monocytogenes, thermotolerant campylobacter, and verocytotoxin producing escherichia coli o157:h7. the performance of different analytical methods used-official culture method (iso), modified bacteriological analytical manual cultural method (mbam), and polymerase chain reaction (pcr)-was evaluated. the presence of mycobacterium avium subsp. paratuber ... | 2012 | 22360646 |
| development of freeze-dried bacteriocin-containing preparations from lactic acid bacteria to inhibit listeria monocytogenes and staphylococcus aureus. | there has been a recent movement to produce and consume "minimally processed" and more "natural" foods through the use of fewer chemical preservatives. the shift to more "natural" foods has resulted in a great interest in the use of bacteriocins from lactic acid bacteria as natural biopreservatives. the objective of this comparative study was to identify bacteriocins that can be produced in low-cost or no-cost dairy-based media (dbm), concentrated using freeze-drying, and applied to cheddar chee ... | 2012 | 26781734 |
| mechanism of nisin, pediocin 34, and enterocin fh99 resistance in listeria monocytogenes. | nisin-, pediocin 34-, and enterocin fh99-resistant variants of listeria monocytogenes atcc 53135 were developed. in an attempt to clarify the possible mechanisms underlying bacteriocin resistance in l. monocytogenes atcc 53135, sensitivity of the resistant strains of l. monocytogenes atcc 53135 to nisin, pediocin 34, and enterocin fh99 in the absence and presence of different divalent cations was assessed, and the results showed that the addition of divalent cations significantly reduced the inh ... | 2012 | 26781732 |
| lack of functional selectin ligand interactions compromises long term tumor protection by cd8+ t cells. | central memory cd8(+) t cells expressing the adhesion molecule cd62l (l-selectin) are potent mediators of anti-cancer immunity due to their ability to proliferate extensively upon antigen re-stimulation. the interaction of selectin with its ligands mediates leukocyte rolling along high endothelial venules. mice deficient in α(1,3) fucosyltransferase iv and vii (ftdko) lack functional l, p and e selectin ligands. thus, we addressed whether the lack of selectin ligand interactions alters tumor pro ... | 2012 | 22359671 |