| kinase suppressor of ras 1 is not required for the generation of regulatory and memory t cells. | the mammalian target of rapamycin (mtor) kinase is a critical regulator of the differentiation of helper and regulatory cd4+ t cells, as well as memory cd8+ t cells. in this study, we investigated the role of the erk signaling pathway in regulating mtor activation in t cells. we showed that activation of erk following tcr engagement is required for sustained mtor complex 1 (mtorc1) activation. absence of kinase suppressor of ras 1 (ksr1), a scaffold protein of the erk signaling pathway, or inhib ... | 2013 | 23431403 |
| chorioamnionitis caused by listeria monocytogenes: a case report of ultrasound features of fetal infection. | maternal listeriosis is often associated with mild symptoms for the patient, but fetal infection can lead to severe adverse perinatal outcome. the most described antenatal symptoms are reduced fetal movements and an abnormal fetal heart rate trace. we present a case of fetal listeriosis suspected by ultrasound findings of fetal gastrointestinal compromise, neonatal diagnosis and outcome. | 2013 | 23429225 |
| mutants of listeriolysin o for enhanced liposomal delivery of macromolecules. | delivery of macromolecules into the cytosolic space of eukaryotic cells is a pressing challenge in biopharmaceutics. macromolecules are often encapsulated into liposomes for protection and improved distribution, but the their size often induces endocytosis of the vehicle at the target site, leading to degradation of the cargo. listeriolysin o is a key virulence factor of listeria monocytogenes that forms pores in the endosomal membrane, ultimately allowing the bacterium to escape into the cytoso ... | 2013 | 23416330 |
| comprehensive update of dalbavancin activity when tested against uncommonly isolated streptococci, corynebacterium spp., listeria monocytogenes, and micrococcus spp. (1357 strains). | dalbavancin is an investigational lipoglycopeptide having an extended serum elimination half-life allowing once-weekly dosing. data from testing 1357 strains of uncommonly isolated species expand the dalbavancin spectrum details as follows (mic50/90): β-haemolytic streptococcal serogroups c, f, and g (≤0.03/≤0.03 μg/ml), 7 viridans group of streptococci (≤0.03/≤0.03-0.06 μg/ml), 5 corynebacterium spp. (0.06/0.12 μg/ml), listeria monocytogenes (0.06/0.12 μg/ml), and micrococcus spp. (≤0.03/≤0.03 ... | 2013 | 23414746 |
| a potential new pathway for pd-l1 costimulation of the cd8-t cell response to listeria monocytogenes infection. | programmed death ligand-1 (pd-l1) is an important negative regulator of t cell immune responses via interactions with pd-1 and cd80. however, pd-l1 can also act as a positive costimulator, but the relevant counterreceptor is not known. we analyzed the role of pd-l1 in cd8-t cell responses to infection with listeria monocytogenes (lm) or vesicular stomatitis virus (vsv). pd-l1 blockade impaired antigen-specific cd8 effector t cell expansion in response to lm, but not to vsv infection, particularl ... | 2013 | 23409193 |
| complete genome sequence of listeria monocytogenes ll195, a serotype 4b strain from the 1983-1987 listeriosis epidemic in switzerland. | the complete genome sequence of listeria monocytogenes ll195, a serotype 4b clinical strain isolated during the 1983-1987 listeriosis epidemic in switzerland, is presented. | 2013 | 23405339 |
| impact of the listeria monocytogenes protein inlc on infection in mice. | the bacterial pathogen listeria monocytogenes causes serious food-borne illnesses in pregnant women and the immunocompromised. l. monocytogenes promotes its internalization into host epithelial cells and then uses an f-actin-dependent motility process to spread from infected cells to surrounding healthy cells. in cultured enterocytes, efficient spread of l. monocytogenes requires the secreted bacterial protein inlc. inlc promotes dissemination by physically interacting with and antagonizing the ... | 2013 | 23403554 |
| listeriolysin o as a strong immunogenic molecule for the development of new anti-tumor vaccines. | the pore-forming toxin listeriolysin o (llo), which is produced by listeria monocytogenes, mediates bacterial phagosomal escape and facilitates bacterial multiplication during infection. this toxin has recently gained attention because of its confirmed role in the controlled and specific modulation of the immune response. currently, cancer immunotherapies are focused on conquering the immune tolerance induced by poorly immunogenic tumor antigens and eliciting strong, lasting immunological memory ... | 2013 | 23399758 |
| processing of recombinant listeria monocytogenes proteins for mhc class i presentation follows a dedicated, high-efficiency pathway. | cd8(+) t lymphocytes recognize short peptides of ∼8-10 aa bound to mhc class i molecules (pmhc) on the surface of apcs. these peptides can be generated from either endogenous proteins synthesized by the biosynthetic machinery of the presenting cell or from exogenously sourced proteins. because much of the research characterizing the mhc class i processing pathway has focused on endogenously synthesized proteins, it is not known whether differences exist in the processing pathway followed by endo ... | 2013 | 23396941 |
| effects of high hydrostatic pressure and varying concentrations of sodium nitrite from traditional and vegetable-based sources on the growth of listeria monocytogenes on ready-to-eat (rte) sliced ham. | the objective of this study was to determine the effect the source of added nitrite and high hydrostatic pressure (hhp) had on the growth of listeria monocytogenes on ready-to-eat (rte) sliced ham. use of 600mpa hhp for 3min resulted in an immediate 3.9-4.3log cfu/g reduction in l. monocytogenes numbers, while use of 400mpa hhp (3min) provided less than 1log cfu/g reduction. with the 600mpa hhp treatment, sliced ham with a conventional concentration of sodium nitrite (200ppm) was not different i ... | 2013 | 23391864 |
| monoclonal antibodies recognizing the surface autolysin ispc of listeria monocytogenes serotype 4b: epitope localization, kinetic characterization, and cross-reaction studies. | listeria monocytogenes serotype 4b is responsible for a high percentage of fatal cases of food-borne infection. in a previous study, we created 15 monoclonal antibodies (mabs) against a ≈ 77 kda antigen that is associated with the cell surface of live l. monocytogenes serotype 4b cells. here we report an extensive characterization of these mabs to further their development as diagnostic reagents. the ≈ 77 kda target antigen was identified by mass spectrometry and n-terminal sequencing to be ispc ... | 2013 | 23390515 |
| improving efficiency of viability-pcr for selective detection of live cells. | viability pcr (v-pcr) as a method to selectively detect intact live cells has gained considerable interest over the last years with an increasing number of applications. the principle is based on treatment of microbiological samples with a viability dye prior to extraction of genomic dna and its amplification. the dye is selectively taken up by membrane-compromised dead cells resulting in the degradation of their dna upon light exposure and therefore inhibition of amplification. although the tre ... | 2013 | 23389080 |
| sculpting mhc class ii-restricted self and non-self peptidome by the class i ag-processing machinery and its impact on th-cell responses. | it is generally assumed that the mhc class i antigen (ag)-processing (cap) machinery - which supplies peptides for presentation by class i molecules - plays no role in class ii-restricted presentation of cytoplasmic ags. in striking contrast to this assumption, we previously reported that proteasome inhibition, tap deficiency or eraap deficiency led to dramatically altered t helper (th)-cell responses to allograft (hy) and microbial (listeria monocytogenes) ags. herein, we tested whether altered ... | 2013 | 23386199 |
| a defined, glucose-limited mineral medium for the cultivation of listeria spp. | members of the genus listeria are fastidious bacteria with respect to their nutritional requirements, and several minimal media described in the literature fail to support growth of all listeria spp. furthermore, strict limitation by a single nutrient, e.g., the carbon source, has not been demonstrated for any of the published minimal media. this is an important prerequisite for defined studies of growth and physiology, including "omics." based on a theoretical analysis of previously published m ... | 2013 | 23377938 |
| genetic determinants for cadmium and arsenic resistance among listeria monocytogenes serotype 4b isolates from sporadic human listeriosis patients. | in listeria monocytogenes serotype 4b isolates from sporadic listeriosis, heavy metal resistance was primarily encountered in certain clonal groups (eci, ecii, and ecia). all arsenic-resistant isolates harbored the arsenic resistance cassette previously identified in pli100; ecia harbored additional arsenic resistance genes and a novel cadmium resistance determinant in a conserved chromosomal locus. | 2013 | 23377929 |
| the surface proteins inla and inlb are interdependently required for polar basolateral invasion by listeria monocytogenes in a human model of the blood-cerebrospinal fluid barrier. | the gram-positive bacterium listeria monocytogenes can enter the human central nervous system and cause life-threatening meningitis. during this process the pathogen has to invade and cross diverse cellular barriers involving the functions of the surface proteins internalin (inla) and inlb. whereas the internalin-dependent crossing of the intestinal epithelium and the fetoplacental barrier have been subject to intensive investigation, limited research elucidating the crossing of the human blood- ... | 2013 | 23376167 |
| in vivo activity of nisin a and nisin v against listeria monocytogenes in mice. | lantibiotics are post-translationally modified antimicrobial peptides, of which nisin a is the most extensively studied example. bioengineering of nisin a has resulted in the generation of derivatives with increased in vitro potency against gram-positive bacteria. of these, nisin v (containing a met21val change) is noteworthy by virtue of exhibiting enhanced antimicrobial efficacy against a wide range of clinical and food-borne pathogens, including listeria monocytogenes. however, this increased ... | 2013 | 23374279 |
| trajectories of listeria-type motility in two dimensions. | force generated by actin polymerization is essential in cell motility and the locomotion of organelles or bacteria such as listeria monocytogenes. both in vivo and in vitro experiments on actin-based motility have observed geometrical trajectories including straight lines, circles, s-shaped curves, and translating figure eights. this paper reports a phenomenological model of an actin-propelled disk in two dimensions that generates geometrical trajectories. our model shows that when the evolution ... | 2012 | 23367971 |
| listeria monocytogenes in donated platelets: a potential transfusion-transmitted pathogen intercepted through screening. | bacterial contamination of blood components is a potentially life-threatening complication of transfusions. in october 2005, the centers for disease control and prevention (cdc) noted four listeria monocytogenes (lm) isolates cultured from four different units of donated apheresis platelets (plts) among lm isolates sent to the cdc national listeria reference laboratory for subtyping as part of routine surveillance activities. | 2013 | 23362824 |
| rapid detection of several foodborne pathogens by f0f1-atpase molecular motor biosensor. | f0f1-atpase within chromatophore was constructed as a molecular motor biosensor through ε-subunit antibody-biotin-streptavidin-biotin-ac5-sulfo-osu system. based on probe-dna specific binding, dna of several foodborne pathogens listeria monocytogenes, salmonella typhimurium, vibrio parahaemolyticus and vibrio cholerae was specifically captured by f0f1-atpase molecular motor biosensors. loads of dna decreased the rotation rate of f0f1-atpase, and led to the decrease of atp synthesis. the detectio ... | 2013 | 23361046 |
| involvement of toso in activation of monocytes, macrophages, and granulocytes. | rapid activation of immune responses is necessary for antibacterial defense, but excessive immune activation can result in life-threatening septic shock. understanding how these processes are balanced may provide novel therapeutic potential in treating inflammatory disease. fc receptors are crucial for innate immune activation. however, the role of the putative fc receptor for igm, known as toso/faim3, has to this point been unclear. in this study, we generated toso-deficient mice and used them ... | 2013 | 23359703 |
| cd11a regulates effector cd8 t cell differentiation and central memory development in response to infection with listeria monocytogenes. | β2 (cd18) integrins with α-chains cd11a, -b, -c, and -d are important adhesion molecules necessary for leukocyte migration and cellular interactions. cd18 deficiency leads to recurrent bacterial infections and poor wound healing due to reduced migration of leukocytes to inflammatory sites. cd8 t cells also upregulate cd11a, cd11b, and cd11c upon activation. however, the role these molecules play for cd8 t cells in vivo is not known. to determine the function of individual β2 integrins, we examin ... | 2013 | 23357382 |
| lateral-flow enzyme immunoconcentration for rapid detection of listeria monocytogenes. | lateral-flow enzyme immunochromatography coupled with an immunomagnetic step was developed for rapid detection of listeria monocytogenes in food matrices. the target bacteria was first separated and concentrated by magnetic nanoparticles containing the enzyme and directly applied to the assay system to induce an antigen-antibody reaction without any additional steps. the color signals produced by an enzyme-substrate reaction at a specific site on the immunostrip were found to be directly proport ... | 2013 | 23354582 |
| brain infection and activation of neuronal repair mechanisms by the human pathogen listeria monocytogenes in the lepidopteran model host galleria mellonella. | listeria monocytogenes the causative agent of the foodborne disease listeriosis in humans often involves fatal brainstem infections leading to meningitis and meningoencephalitis. we recently established the larvae of the greater wax moth (galleria mellonella) as a model host for the investigation of l. monocytogenes pathogenesis and as a source of peptides exhibiting anti-listeria-activity. here we show that g. mellonella can be used to study brain infection and its impact on larval development ... | 2013 | 23348912 |
| fluorescence amplified fragment length polymorphism compared to pulsed field gel electrophoresis for listeria monocytogenes subtyping. | listeriosis is a severe infection which mainly affects pregnant women, neonates and immuno-compromised adults. anses's laboratory for food safety has been the european union reference laboratory (eurl) for l. monocytogenes in the food chain since 2006. pulsed field gel electrophoresis (pfge) is routinely used in the eurl for the surveillance of l. monocytogenes isolated from foods, animals and the environment. one of the main eurl activities is to evaluate alternative molecular subtyping methods ... | 2013 | 23347599 |
| pathogenesis of listeriosis during pregnancy. | listeria monocytogenes causes several clinical manifestations in humans and domestic animals. this bacterium is a saprophyte in soil and ensiled feeds, which are sources of infection for food producing animals (i.e. ruminants). the most common route of infection for people is via ingestion of contaminated ready-to-eat food products such as produce, soft cheeses and deli meats. in the united states, l. monocytogenes causes relatively few cases of clinical disease compared to other food-borne path ... | 2013 | 23347534 |
| mudpit profiling reveals a link between anaerobic metabolism and the alkaline adaptive response of listeria monocytogenes egd-e. | listeria monocytogenes is a foodborne human pathogen capable of causing life-threatening disease in susceptible populations. previous proteomic analysis we performed demonstrated that different strains of l. monocytogenes initiate a stringent response when subjected to alkaline growth conditions. here, using multidimensional protein identification technology (mudpit), we show that in l. monocytogenes egd-e this response involves an energy shift to anaerobic pathways in response to the extracellu ... | 2013 | 23342094 |
| retraction statement: performance of three culture media commonly used for detecting listeria monocytogenes. | | 2012 | 23339828 |
| survival of food-borne pathogens on natural black table olives after post-processing contamination. | the aim of this study was to evaluate the survival of salmonella, escherichia coli o157:h7, listeria monocytogenes, and staphylococcus aureus on natural black olives during aerobic storage without brine and to conclude about the safety of this fermented food. fermented black olives (ph 3.95; nacl 6.02%) were artificially inoculated with 4 strains of salmonella enterica ser. enteritidis, 4 strains of s. enterica ser. typhimurium, 3 strains of e. coli o157:h7, 3 strains of l. monocytogenes, and 3 ... | 2013 | 23334099 |
| antibacterial activity of veronica montana l. extract and of protocatechuic acid incorporated in a food system. | this study was designed to evaluate the antibacterial activity of the veronica montana l. water extract and its main phenolic compound, protocatechuic acid. the antibacterial activity was determined by microdilution assay against six strains of gram-positive and gram-negative bacteria. listeria monocytogenes was the most sensitive of the tested bacterial species. antibacterial preserving properties of protocatechuic acid were also evaluated after its incorporation in cream cheese, using l. monoc ... | 2013 | 23333716 |
| disruption of lmo1386, a putative dna translocase gene, affects biofilm formation of listeria monocytogenes on abiotic surfaces. | the distribution and survival of listeria monocytogenes (l. monocytogenes) in food processing environment is linked to its ability to form biofilms, however the genetic mechanisms remain unclear. in our previous study, a himar1 mariner-based transposon mutagenesis was performed and 42 mutants were confirmed to have reduced biofilm formation. among the 42 biofilm deficient mutants, two isolates (s25-10c and s55-1d) harbored single insertion in lmo1386, a gene encoding a putative dna translocase. ... | 2013 | 23333341 |
| [invasive listeriosis in valencian community, spain, during the period 2008-2010]. | listeria monocytogenes is an uncommon cause of disease, although in certain age groups (infants, elderly, pregnant women and immunocompromised) may be an important cause of meningoencephalitis, and bacteremia. the aim of this study was to detect the incidence of invasive listeriosis cases found in valencia during a period of 3 years. | 2012 | 23325139 |
| microglial activation and expression of immune-related genes in a rat ex vivo nervous system model after infection with listeria monocytogenes. | a wide variety of microorganisms has previously been identified as causes of brain infection. among them, listeria monocytogenes has a particular tropism for the central nervous system. to gain knowledge about the immune response elicited by l. monocytogenes in the brain, we used a rat ex vivo organotypic nervous system culture as a model for listeria infection. scanning electron microscopy (sem) revealed that activated microglial cells showing a typical amoeboid morphology are quickly recruited ... | 2013 | 23322603 |
| low potential virulence associated with mutations in the inla and prfa genes in listeria monocytogenes isolated from raw retail poultry meat. | packaged raw foods can represent a potential source of listeria monocytogenes contamination when opened at home, and listeriosis is associated with the consumption of undercooked raw foods. the aim of this study was to characterize a group of l. monocytogenes strains isolated from 56 packages of raw chicken meat from a single brand in order to determine the diversity of the strains that dominate in a particular food over time, as well as their pathogenic potential. forty (71%) samples were found ... | 2013 | 23317868 |
| bactericidal activities of health-promoting, food-derived powders against the foodborne pathogens escherichia coli, listeria monocytogenes, salmonella enterica, and staphylococcus aureus. | we evaluated the relative bactericidal activities (ba(50) ) of 10 presumed health-promoting food-based powders (nutraceuticals) and, for comparison, selected known components against the following foodborne pathogens: escherichia coli o157:h7, salmonella enterica, listeria monocytogenes, and staphylococcus aureus. the relative activities were evaluated using quantitative bactericidal activity [(ba(50) value, defined as the percentage of the sample in the assay mixture that resulted in a 50% decr ... | 2013 | 23317422 |
| quantification of the extracellular matrix of the listeria monocytogenes biofilms of different phylogenic lineages with optimization of culture conditions. | the purpose of this study was to quantify the extracellular matrix of listeria monocytogenes biofilm. a preliminary study was carried out to establish a relationship between phylogenetic lineage of 27 strains and their ability to form biofilm in various conditions. | 2013 | 23317349 |
| examination of food chain-derived listeria monocytogenes strains of different serotypes reveals considerable diversity in inla genotypes, mutability, and adaptation to cold temperatures. | listeria monocytogenes strains belonging to serotypes 1/2a and 4b are frequently linked to listeriosis. while inla mutations leading to premature stop codons (pmscs) and attenuated virulence are common in 1/2a, they are rare in serotype 4b. we observed pmscs in 35% of l. monocytogenes isolates (n = 54) recovered from the british columbia food supply, including serotypes 1/2a (30%), 1/2c (100%), and 3a (100%), and a 3-codon deletion (amino acid positions 738 to 740) seen in 57% of 4b isolates fro ... | 2013 | 23315746 |
| development of a random genomic dna microarray for the detection and identification of listeria monocytogenes in milk. | we developed a dna microarray that contains random genomic dna fragments of listeria monocytogenes, validated its diagnostic abilities using cells grown in laboratory media and milk, and established enrichment conditions for detection of a low population of l. monocytogenes in milk. genomic dna of l. monocytogenes strain atcc 19111 was fractionated by agarose gel electrophoresis after being cleaved using several different pairs of restriction enzymes. sixty dna fragments of different sizes were ... | 2013 | 23313851 |
| new data, strategies, and insights for listeria monocytogenes dose-response models: summary of an interagency workshop, 2011. | listeria monocytogenes is a leading cause of hospitalization, fetal loss, and death due to foodborne illnesses in the united states. a quantitative assessment of the relative risk of listeriosis associated with the consumption of 23 selected categories of ready-to-eat foods, published by the u.s. department of health and human services and the u.s. department of agriculture in 2003, has been instrumental in identifying the food products and practices that pose the greatest listeriosis risk and h ... | 2013 | 23311571 |
| antilisterial properties of marinades during refrigerated storage and microwave oven reheating against post-cooking inoculated chicken breast meat. | this study evaluated growth of listeria monocytogenes inoculated on cooked chicken meat with different marinades and survival of the pathogen as affected by microwave oven reheating. during aerobic storage at 7 °c, on days 0, 1, 2, 4, and 7, samples were reheated by microwave oven (1100 w) for 45 or 90 s and analyzed microbiologically. l. monocytogenes counts on nonmarinated (control) samples increased (p < 0.05) from 2.7 ± 0.1 (day-0) to 6.9 ± 0.1 (day-7) log cfu/g during storage. initial (day- ... | 2013 | 23311403 |
| ampkα1: a glucose sensor that controls cd8 t-cell memory. | the adenosine monophosphate-activated protein kinase (ampk) is activated by antigen receptor signals and energy stress in t cells. in many cell types, ampk can maintain energy homeostasis and can enforce quiescence to limit energy demands. we consequently evaluated the importance of ampk for controlling the transition of metabolically active effector cd8 t lymphocytes to the metabolically quiescent catabolic memory t cells during the contraction phase of the immune response. we show that ampkα1 ... | 2013 | 23310952 |
| what is the incubation period for listeriosis? | listeriosis is a foodborne infection with a low incidence but a high case fatality rate. unlike common foodborne diseases, the incubation period can be long. the first incubation periods were documented during a large listeriosis outbreak published in 1987 by linnan and al. in the new england journal of medicine (range: 3 days to 70 days). data on the incubation period of listeriosis are scarce. our study aim was to estimate precisely the incubation period of listeriosis using available data sin ... | 2013 | 23305174 |
| listeria monocytogenes inoculation protects mice against blood-stage plasmodium yoelii infection. | listeria monocytogenes (lm) has been used as the adjuvant or vector for tumor and viral vaccine for its capability of eliciting all aspects of cell-mediated immunity including t cell activation and interferon-gamma (ifn-γ) production. these effector components play critical roles in the protection against plasmodium infection in both human malaria and mouse models. therefore, immune response induced by lm infection may benefit the defense against malaria. to test this hypothesis, we employed blo ... | 2013 | 23303295 |
| extraction of cell wall-bound teichoic acids and surface proteins from listeria monocytogenes. | gram-positive bacteria contain a cell wall consisting of a thick peptidoglycan layer decorated with surface proteins and polysaccharide-based polymers. the latter include the wall teichoic acids (wtas), which are anionic glycopolymers covalently linked to the peptidoglycan matrix. they are constituted by a long backbone containing sugars of various sizes (trioses to hexoses) which can be reduced (polyols as in listeria) or oxidized (uronic acids) and can undergo a variety of species- or often st ... | 2013 | 23299742 |
| multiple abscesses of the left brain hemisphere due to listeria monocytogenes in an immunocompromised patient: a case report. | we describe a case of brain abscesses in a cirrhotic and diabetic 57-year-old woman showing fever, aphasia, right hemiparesis and seizures. neuroradiological investigation revealed unilateral cerebritis evolving in multiple abscesses. from blood and surgical drainage samples listeria monocytogenes grew in pure culture. despite decompressive craniotomy, the patient died two months after hospital admission. | 2012 | 23299068 |
| salt stress-induced invasiveness of major listeria monocytogenes serotypes. | of the 13 serotypes, 4b serotype strains are responsible for the majority of recorded invasive listeriosis outbreaks, although some recent listeriosis outbreaks have been attributed to strains of serotypes 1/2a and 1/2b. virulence and response to osmotic stress in 41 listeria monocytogenes strains representing serotypes 1/2a, 1/2b and 4b was investigated. it was found that serotype 4b and 1/2b strains exhibited highest invasion efficiency and formed largest plaques in ht-29 cell monolayer. invas ... | 2013 | 23294476 |
| exoproteomic analysis of the seca2-dependent secretion in listeria monocytogenes egd-e. | as part of the sec translocase, the accessory atpase seca2 is present in some pathogenic gram-positive bacteria. in listeria monocytogenes, deletion of seca2 results in filamentous cells that form rough colonies and have lower virulence. however, only a few proteins have been identified that are secreted by this pathway. this investigation aims to provide the first exoproteomic analysis of the seca2-dependent secretion in l. monocytogenes egd-e. by using media and temperatures relevant to bacter ... | 2013 | 23291529 |
| inactivation by lemon juice of escherichia coli o157:h7, salmonella enteritidis, and listeria monocytogenes in beef marinating for the ethnic food kelaguen. | lemon juice, a major source of acidulant citric acid, is frequently used in the preparation of ethnic foods. raw or partially cooked meats are marinated with lemon juice in the preparation of a popular chamorro dish called kelaguen, which is, unfortunately, strongly associated with foodborne illness outbreaks in guam. we investigated the efficacy of lemon juice in reducing numbers of escherichia coli o157:h7, salmonella enteritidis, and listeria monocytogenes at stationary phase during marinatio ... | 2013 | 23290245 |
| crig signals induce anti-intracellular bacterial phagosome activity in a chloride intracellular channel 3-dependent manner. | macrophages provide a first line of defense against bacterial infection by engulfing and killing invading bacteria, but intracellular bacteria such as listeria monocytogenes (lm) can survive in macrophages by various mechanisms of evasion. complement receptor of the immunoglobulin (crig), a c3b receptor, binds to c3b on opsonized bacteria and facilitates clearance of the bacteria by promoting their uptake. we found that crig signaling induced by agonistic anti-crig mab enhanced the killing of in ... | 2013 | 23280470 |
| incorporation of listeria monocytogenes strains in raw milk biofilms. | biofilms develop successively on devices of milk production without sufficient cleaning and originate from the microbial community of raw milk. the established biofilm matrices enable incorporation of pathogens like listeria monocytogenes, which can cause a continuous contamination of food processing plants. l. monocytogenes is frequently found in raw milk and non-pasteurized raw milk products and as part of a biofilm community in milk meters and bulk milk tanks. the aim of this study was to ana ... | 2013 | 23279814 |
| severe invasive listeriosis--case report. | listeriosis is a rare food borne infection which, in the invasive form, presents as bloodstream infection, central nervous system infection, materno-fetal infection, or focal infection. certain immunosuppressive conditions have been identified as risk factors for severe invasive disease. the invasive forms of listeriosis are associated with a high case fatality rate. we present the case of a 62-year-old male with an unremarkable medical history admitted to the iasi infectious diseases hospital f ... | 2013 | 23272533 |
| influence of sterilized human fecal extract on the sensitivity of salmonella enterica atcc 13076 and listeria monocytogenes atcc 15313 to enrofloxacin. | there is much debate on whether continuous exposure of commensal bacteria and potential pathogens residing in the human intestinal tract to low levels of antimicrobial agents from treated food animals pose a public health concern. to investigate antimicrobial effects on bacteria under colonic conditions, we studied resistance development in salmonella enterica and listeria monocytogenes exposed to enrofloxacin in the presence of fecal extract. the bacteria were incubated at 37 °c in mueller-hint ... | 2013 | 27029316 |
| spontaneous bacterial peritonitis caused by listeria monocytogenes associated with ascitic fluid lymphocytosis: a case report and review of current empiric therapy. | spontaneous bacterial peritonitis (sbp) is a potentially deadly complication of ascites. we describe a case of sbp caused by listeria monocytogenes in a patient with alcoholic cirrhosis. this was associated with the unusual finding of ascitic fluid lymphocytosis, which previously had only been associated with tuberculoid or malignant ascites. given increasing rates of cefotaxime-resistant sbp alongside the possibility of listeriosis, the use of cefotaxime as first-line therapy in sbp should be r ... | 2013 | 25383227 |
| anti-pd-1 antibody significantly increases therapeutic efficacy of listeria monocytogenes (lm)-llo immunotherapy. | one of the significant tumor immune escape mechanisms and substantial barrier for successful immunotherapy is tumor-mediated inhibition of immune response through cell-to-cell or receptor/ligand interactions. programmed death receptor-1 (pd-1) interaction with its ligands, pd-l1 and pd-l2, is one of the important strategies that many tumors employ to escape immune surveillance. upon pd-ls binding to pd-1, t cell receptor (tcr) signaling is dampened, causing inhibition of proliferation, decreased ... | 2013 | 24829751 |
| comparative lethality kinetic curves and predictive models of f-value for listeria monocytogenes using different sanitizers. | the objective of this work was to evaluate the kinetic of inactivation of listeria monocytogenes using peracetic acid, chlorhexidine, and organic acids as active agent, determining the respective d-, z-, and f-values. from our knowledge, these important results from an industrial view point are not available in the current literature, mainly for organic acids, pointing out the main contribution of the present work. lower d-values were obtained for peracetic acid and chlorhexidine, compared with ... | 2013 | 24804011 |
| effects of growth phase and temperature on σb activity within a listeria monocytogenes population: evidence for rsbv-independent activation of σb at refrigeration temperatures. | the alternative sigma factor σb of listeria monocytogenes is responsible for regulating the transcription of many of the genes necessary for adaptation to both food-related stresses and to conditions found within the gastrointestinal tract of the host. the present study sought to investigate the influence of growth phase and temperature on the activation of σb within populations of l. monocytogenes egd-e wild-type, δsigb, and δrsbv throughout growth at both 4°c and 37°c, using a reporter fusion ... | 2014 | 24734238 |
| of mice and men: dissecting the interaction between listeria monocytogenes internalin a and e-cadherin. | we report a study of the interaction between internalin a (inla) and human or murine e-cadherin (ecad). inla is used by listeria monocytogenes to internalize itself into host cell, but the bacterium is unable to invade murine cells, which has been attributed to the difference in sequence between hecad and mecad. using molecular dynamics simulations, mm/gbsa free energy calculations, hydrogen bond analysis, water characterization and umbrella sampling, we provide a complete atomistic picture of t ... | 2013 | 24688730 |
| how the fly balances its ability to combat different pathogens. | health is a multidimensional landscape. if we just consider the host, there are many outputs that interest us: evolutionary fitness determining parameters like fecundity, survival and pathogen clearance as well as medically important health parameters like sleep, energy stores and appetite. hosts use a variety of effector pathways to fight infections and these effectors are brought to bear differentially. each pathogen causes a different disease as they have distinct virulence factors and niches ... | 2012 | 23271964 |
| occam's razor and probiotics activity on listeria monocytogenes. | | 2013 | 23271812 |
| transfer of cd8+ t cell memory using bcl-2 as a marker. | the processes that regulate t cell memory generation are important for therapeutic design and the immune response to disease. however, what allows a subset of effector t cells to survive the contraction period to become memory cells is incompletely understood. the bcl-2 family is critical for t cell survival, and bcl-2 has been proposed to be important for the survival of memory cells. however, previous studies have relied on double-knockout models, potentially skewing the role of bcl-2, and the ... | 2013 | 23269245 |
| polyphasic characterization and genetic relatedness of low-virulence and virulent listeria monocytogenes isolates. | currently, food regulatory authorities consider all listeria monocytogenes isolates as equally virulent. however, an increasing number of studies demonstrate extensive variations in virulence and pathogenicity of l. monocytogenes strains. up to now, there is no comprehensive overview of the population genetic structure of l. monocytogenes taking into account virulence level. we have previously demonstrated that different low-virulence strains exhibit the same mutations in virulence genes suggest ... | 2012 | 23267677 |
| identification of an optimized panel of variable number tandem-repeat (vntr) loci for listeria monocytogenes typing. | listeria monocytogenes is an important worldwide foodborne pathogen. for listeriosis outbreak investigations, an optimal multiple-locus variable-number-tandem-repeat analysis typing panel was identified from 4 previously reported schemes. the optimal combination of loci consisted of 9 loci (lmv6, lmv1, lmv2, lm11, lm10, lmv7, lm32, lm-tr6, and lm23), which produced the same level of differentiation ability simpson index of 0.9914 as that of all 14 loci combined in the previous 4 reports. this pa ... | 2013 | 23265294 |
| exploration of the role of the non-coding rna sbre in l. monocytogenes stress response. | sbre is a ncrna in listeria monocytogenes, reported to be up-regulated by the alternative sigma factor σb. initial quantitative rt-pcr (qrt-pcr) experiments on parent strains and isogenic δsigb strains demonstrated σb-dependent expression of sbre across the four l. monocytogenes lineages and in l. innocua. microarray and proteomics (mdlc/ms/ms with itraq labeling) experiments with the l. monocytogenes parent strain and an isogenic δsbre strain identified a single gene (lmo0636) and two proteins ... | 2012 | 23263668 |
| nanodispersed eugenol has improved antimicrobial activity against escherichia coli o157:h7 and listeria monocytogenes in bovine milk. | there has been great interest in intervention strategies based on plant essential oils to control pathogens such as escherichia coli o157:h7 and listeria monocytogenes (lm). however, the poor solubility of essential oils in water makes it difficult to disperse evenly in food matrices, impacting food quality and antimicrobial efficacy. in the present study, eugenol was dispersed in nanocapsules prepared with conjugates of whey protein isolate (wpi) and maltodextrin (md, of various chain lengths). ... | 2013 | 23261812 |
| novel epidemic clones of listeria monocytogenes, united states, 2011. | we identified a novel serotype 1/2a outbreak strain and 2 novel epidemic clones of listeria monocytogenes while investigating a foodborne outbreak of listeriosis associated with consumption of cantaloupe during 2011 in the united states. comparative analyses of strains worldwide are essential to identification of novel outbreak strains and epidemic clones. | 2013 | 23260778 |
| listeriosis outbreaks and associated food vehicles, united states, 1998-2008. | listeria monocytogenes, a bacterial foodborne pathogen, can cause meningitis, bacteremia, and complications during pregnancy. this report summarizes listeriosis outbreaks reported to the foodborne disease outbreak surveillance system of the centers for disease control and prevention during 1998-2008. the study period includes the advent of pulsenet (a national molecular subtyping network for outbreak detection) in 1998 and the listeria initiative (enhanced surveillance for outbreak investigation ... | 2013 | 23260661 |
| rhombencephalitis due to listeria monocytogenes: a case study. | | 2014 | 23260449 |
| [meningitis due to listeria monocytogenes in adults]. | listeria monocytogenes is the third most common cause of community-acquired bacterial meningitis in adults. | 2013 | 23250677 |
| murine guanylate binding protein 2 (mgbp2) controls toxoplasma gondii replication. | ifn-γ orchestrates the host response against intracellular pathogens. members of the guanylate binding proteins (gbp) comprise the most abundant ifn-γ-induced transcriptional response. mgbps are gtpases that are specifically up-regulated by ifn-γ, other proinflammatory cytokines, toll-like receptor agonists, as well as in response to listeria monocytogenes and toxoplasma gondii infection. mgbp2 localizes at the parasitophorous vacuole (pv) of t. gondii; however, the molecular function of mgbp2 a ... | 2013 | 23248289 |
| delayed cerebral thrombosis in bacterial meningitis: a prospective cohort study. | to study the incidence and clinical characteristics of delayed cerebral thrombosis in bacterial meningitis patients. | 2013 | 23248040 |
| synergistic combinations of high hydrostatic pressure and essential oils or their constituents and their use in preservation of fruit juices. | this work addresses the inactivation achieved with escherichia coli o157:h7 and listeria monocytogenes egd-e by combined processes of high hydrostatic pressure (hhp) and essential oils (eos) or their chemical constituents (ccs). hhp treatments (175-400 mpa for 20 min) were combined with 200 μl/l of each eo (citrus sinensis l., citrus lemon l., citrus reticulata l., thymus algeriensis l., eucalyptus globulus l., rosmarinus officinalis l., mentha pulegium l., juniperus phoenicea l., and cyperus lo ... | 2013 | 23246609 |
| expanding roles for gilt in immunity. | gamma-interferon-inducible lysosomal thiol reductase (gilt), a thioredoxin-related oxidoreductase, functions in mhc class ii-restricted antigen processing and mhc class i-restricted cross-presentation by reducing disulfide bonds of endocytosed proteins and facilitating their unfolding and optimal degradation. however, recent reports have greatly expanded our understanding of gilt's function. several studies of gilt and antigen processing have shown that the influence of gilt on the peptide reper ... | 2013 | 23246037 |
| il-6 controls the innate immune response against listeria monocytogenes via classical il-6 signaling. | the cytokine il-6 plays a protective role in immune responses against bacterial infections. however, the mechanisms of il-6-mediated protection are only partially understood. il-6 can signal via the il-6r complex composed of membrane-bound il-6rα (mil-6rα) and gp130. owing to the restricted expression of mil-6rα, classical il-6 signaling occurs only in a limited number of cells such as hepatocytes and certain leukocyte subsets. il-6 also interacts with soluble il-6rα proteins and these il-6/solu ... | 2013 | 23241882 |
| (d)-amino acid chemical reporters reveal peptidoglycan dynamics of an intracellular pathogen. | peptidoglycan (pg) is an essential component of the bacterial cell wall. although experiments with organisms in vitro have yielded a wealth of information on pg synthesis and maturation, it is unclear how these studies translate to bacteria replicating within host cells. we report a chemical approach for probing pg in vivo via metabolic labeling and bioorthogonal chemistry. a wide variety of bacterial species incorporated azide and alkyne-functionalized d-alanine into their cell walls, which we ... | 2013 | 23240806 |
| current knowledge and perspectives on biofilm formation: the case of listeria monocytogenes. | listeriosis is a rare, serious, and mainly food-borne infection caused by the bacterium listeria monocytogenes. this food-borne infection primarily affects pregnant women and immunologically compromised individuals. l. monocytogenes is recognized as a problem for the food industry, mainly due to its environmental persistence, attributed in part to its ability to form biofilms. biofilms are microbial communities adhered to biotic or abiotic surfaces coated by self-produced extracellular polymers. ... | 2013 | 23233205 |
| lipid-labeling facilitates a novel magnetic isolation procedure to characterize pathogen-containing phagosomes. | here we describe a novel approach for the isolation and biochemical characterization of pathogen-containing compartments from primary cells: we developed a lipid-based procedure to magnetically label the surface of bacteria and visualized the label by scanning and transmission electron microscopy (sem, tem). we performed infection experiments with magnetically labeled mycobacterium avium, m. tuberculosis and listeria monocytogenes and isolated magnetic bacteria-containing phagosomes using a stro ... | 2013 | 23231467 |
| prevalence and level of listeria monocytogenes and other listeria sp. in ready-to-eat minimally processed and refrigerated vegetables. | minimally processed and refrigerated vegetables can be contaminated with listeria species bacteria including listeria monocytogenes due to extensive handling during processing or by cross contamination from the processing environment. the objective of this study was to examine the microbiological quality of ready-to-eat minimally processed and refrigerated vegetables from supermarkets in osijek, croatia. 100 samples of ready-to-eat vegetables collected from different supermarkets in osijek, croa ... | 2013 | 23225207 |
| listeria monocytogenes cholecystitis: a possible new syndrome. | the u.s. food and drug administration recently added potentially fatal listeria monocytogenes infection to the list of opportunistic infections that can occur in patients who receive tumor necrosis factor inhibitor therapy. in this study, the first reported case of l monocytogenes cholecystitis associated with etanercept use is described. it also appears that tumor necrosis factor inhibitor therapy likely increases the risk for listeria cholecystitis. clinicians need to be aware of this associat ... | 2013 | 23221515 |
| listeria monocytogenes septic arthritis in a patient treated with mycophenolate mofetil for polyarteritis nodosa: a case report and review of the literature. | listeriosis can be a cause of arthritis. here, we present a case of listeria monocytogenes septic arthritis of the right hip in a 66-year-old male treated with mycophenolate mofetil for polyarteritis nodosa. so far, septic arthritis due to this microorganism has not been reported in patients treated with mycophenolate mofetil. we review the literature of l. monocytogenes septic arthritis and discuss the role of mycophenolate mofetil treatment in precipitating listeriosis. | 2013 | 23218550 |
| a multiple-locus variable-number tandem repeat analysis (mlva) of listeria monocytogenes isolated from norwegian salmon-processing factories and from listeriosis patients. | the objective of this study was to characterize listeria monocytogenes isolated from farmed atlantic salmon (salmo salar) and the processing environment in three different norwegian factories, and compare these to clinical isolates by multiple-locus variable-number tandem repeat analysis (mlva). the 65 l. monocytogenes isolates obtained gave 15 distinct mlva profiles. there was great heterogeneity in the distribution of mlva profiles in factories and within each factory. nine of the 15 mlva prof ... | 2013 | 23218175 |
| well-known but rare pathogen in neonates: listeria monocytogenes. | listeria monocytogenes is a very important life-threatening bacteria in certain risk groups such as neonates, pregnant women, elderly people, transplant recipients and others with impaired cell-mediated immunity. however, its infections are very rare in healthy children. reports of listeriosis in newborn period are limited. we report a case of neonatal listeriosis with erythematous rash, intractable convulsions, severe early neonatal sepsis, disseminated intravascular coagulation, multiple organ ... | 2012 | 23090810 |
| evidence of extensive diversity in bacterial adherence mechanisms that exploit unanticipated stainless steel surface structural complexity for biofilm formation. | three protease-resistant bioorganic 304 stainless steel surfaces were created through the reaction of synthetic peptides consisting of the d-enantiomeric isomer (d-k122-4), the retro-inverso d-enantiomeric isomer (ri-k122-4), and a combination of the two peptides (d+ri) of the pseudomonas aeruginosa pila receptor binding domain with steel surfaces. the peptides used to produce the new materials differ only in handedness of their three-dimensional structure, but they reacted with the steel to yie ... | 2013 | 23212080 |
| shelf-life extension and sanitation of fresh pork loin by e-beam treatment. | the usefulness of electron beam (e-beam) irradiation to increase the shelf life of whole fresh pork loin stored at 4°c has been studied. the shelf life was extended from 5 to 11 and 20 days after the application of 1 and 2 kgy, respectively. if a temperature abuse situation were to occur during product distribution (e.g., increase to 8°c), the shelf life would be extended from 3 to 8 and 15 days, respectively, after application of the same doses. when considering listeria monocytogenes from a pu ... | 2012 | 23212015 |
| growth and filamentation of cold-adapted, log-phase listeria monocytogenes exposed to salt, acid, or alkali stress at 3°c. | in canada, there is a zero tolerance for listeria in a 125-g sample of product in which growth of listeria monocytogenes can occur, and a limit of ≤100 cfu/g in ready-to-eat (rte) food products that support limited growth during the stated shelf life and/or rte refrigerated foods with a shelf life of ≤5 days. l. monocytogenes can form filaments in response to ph and osmotic, atmospheric, and temperature stress, which can result in an underestimation of the risk of rte foods as filaments form sin ... | 2012 | 23212010 |
| retrospective analysis of a listeria monocytogenes contamination episode in raw milk goat cheese using quantitative microbial risk assessment tools. | in 2005, the belgian authorities reported a listeria monocytogenes contamination episode in cheese made from raw goat's milk. the presence of an asymptomatic shedder goat in the herd caused this contamination. on the basis of data collected at the time of the episode, a retrospective study was performed using an exposure assessment model covering the production chain from the milking of goats up to delivery of cheese to the market. predictive microbiology models were used to simulate the growth ... | 2012 | 23212008 |
| a comparison of enrichment and direct-plating methods for isolation of listeria monocytogenes from surface water. | listeria monocytogenes is a facultative intracellular pathogen distributed in the environment. reference isolation methods include an enrichment step. we compared 2 protocols: one based on enrichment and the other on direct plating, with respect to the efficiency of isolation of l. monocytogenes from surface water samples. from march to may 2006, 126 surface water samples were collected biweekly from 24 discrete sampling sites located in the south nation river basin (ontario). presumptive lister ... | 2012 | 23210998 |
| antibacterial activity and composition of essential oils from pelargonium graveolens l'her and vitex agnus-castus l. | essential oils are volatile compounds that have been used since middle ages as antimicrobial, anti-inflammatory, sedative, local anesthetic and food flavoring agents. in the current study, essential oils of pelargonium graveolens l'her and vitex agnus-castus l. were analyzed for their antibacterial activities. | 2012 | 23205247 |
| characterization of listeria monocytogenes isolated from ganges water, human clinical and milk samples at varanasi, india. | listeria monocytogenes isolated from ganges water, human clinical and milk samples were characterized by antibiotic susceptibility, serotype identification, detection of virulence genes and eric- and rep-pcr fingerprint analyses. all isolates were uniformly resistant to ampicillin, except two isolates, and showed variable resistance to gentamicin, cotrimoxazole, ofloxacin, rifampicin and tetracycline. of the 20 isolates found positive for pathogens, seven (four human and three water isolates) be ... | 2013 | 23201044 |
| multiplex fiber optic biosensor for detection of listeria monocytogenes, escherichia coli o157:h7 and salmonella enterica from ready-to-eat meat samples. | listeria monocytogenes, escherichia coli o157:h7 and salmonella enterica are the most common foodborne bacterial pathogens and are responsible for many outbreaks. therefore, multiplex detection of these three using a single assay platform is highly desirable. the objective was to develop and optimize a fiber optic sensor for simultaneous detection of these three from food. the streptavidin coated optical waveguides were immobilized with biotinylated polyclonal antibodies and exposed to the bacte ... | 2013 | 23200648 |
| antagonistic effect of pseudomonas graminis cpa-7 against foodborne pathogens in fresh-cut apples under simulated commercial conditions. | recently, we reported that the application of the strain cpa-7 of pseudomonas graminis, previously isolated from apple, could reduce the population of foodborne pathogens on minimally processed (mp) apples and peaches under laboratory conditions. therefore, the objective of the present work was to find an antioxidant treatment and a packaging atmosphere condition to improve cpa-7 efficacy in reducing a cocktail of four salmonella and five listeria monocytogenes strains on mp apples under simulat ... | 2013 | 23200645 |
| two-dimensional fluorescence difference gel electrophoresis analysis of listeria monocytogenes submitted to a redox shock. | the influence of redox alteration on the growth and proteomic pattern of listeria monocytogenes was investigated. a redox shock was induced in cultures by addition of 3mm ferricyanide (fecn) and 6mm dithiothreitol (dtt) to increase or to decrease respectively the redox potential naturally occurring at the beginning of growth. in both conditions, the reducing and oxidizing redox shock had a strong influence, decreasing the maximum growth rate by half compared to a control culture. the proteomic a ... | 2013 | 23195917 |
| antibacterial peptides, probiotic properties and biopreservative efficacy of native bacillus species isolated from different food sources. | in this study, we evaluated the occurrence of antibacterial peptide (abp)-producing bacillus spp. in fermented foods. among 78 isolated cultures, 25 potential abp-producing stains were selected and differentiated genotypically and phenotypically. the 16s rrna gene sequence homology, in combination with morphological, physiological and biochemical characteristics, was used for the identification of the isolates. the isolates exhibited inhibitory activity against both gram-positive and gram-negati ... | 2012 | 26782188 |
| hierarchical shrinkage priors and model fitting for high-dimensional generalized linear models. | abstract genetic and other scientific studies routinely generate very many predictor variables, which can be naturally grouped, with predictors in the same groups being highly correlated. it is desirable to incorporate the hierarchical structure of the predictor variables into generalized linear models for simultaneous variable selection and coefficient estimation. we propose two prior distributions: hierarchical cauchy and double-exponential distributions, on coefficients in generalized linear ... | 2012 | 23192052 |
| comparing organic acids and salt derivatives as antimicrobials against selected poultry-borne listeria monocytogenes strains in vitro. | this article reports on the antilisterial properties of selected organic acids and salt derivatives in order to suggest possible alternatives in food preservation and pathogen control in the poultry meat processing industry. the susceptibility of two listeria monocytogenes isolates was assessed against five organic acids (lactic, acetic, malic, citric, and propionic) and two acid-salt derivatives (sorbic acid [potassium salt] and benzoic acid [sodium salt]) across a series of ph environments. mi ... | 2012 | 23190165 |
| bacterial load and inflammation in fetal tissues is not dependent on il-17a or il-22 in 10-14 day pregnant mice infected with listeria monocytogenes. | in this study, we first assessed the effect of intragastric infection of pregnant mice with listeria monocytogenes on relative expression of select genes associated with t cell subsets. relative gene expression was moderately increased in placental tissues for ifnγ, il-4, il-17a, il-22, cd3, and foxp3. to assess the roles of il-17a and il-22 in resistance to listeriosis during pregnancy, we compared the severity of maternal and fetal infection in il-17a((-/-)), il-22((-/-)), and il-17a((-/-))/il ... | 2013 | 23178254 |
| identification of a ferritin-like protein of listeria monocytogenes as a mediator of β-lactam tolerance and innate resistance to cephalosporins. | the food-borne pathogen listeria monocytogenes is the causative agent of listeriosis. the β-lactam antibiotics penicillin g and ampicillin are the current drugs of choice for the treatment of listerial infections. while isolates of l. monocytogenes are susceptible to these antibiotics, their action is only bacteriostatic and consequently, this bacterium is regarded as tolerant to β-lactams. in addition, l. monocytogenes has a high level of innate resistance to the cephalosporin family of β-lacta ... | 2012 | 23176286 |
| evaluating the use of fatty acid profiles to differentiate human pathogenic and nonpathogenic listeria species. | listeria monocytogenes is a gram-positive human pathogen that is responsible for serious infections in immunocompromised individuals and pregnant women. because of recent epidemics caused by food contaminated with l. monocytogenes, rapid methods for the detection of this pathogen in food are of interest. capillary gas chromatography with flame ionization detection (gc-fid) was used to determine the cellular fatty acid profiles of six species of listeria. the six different species are l. monocyto ... | 2012 | 23175980 |
| listeriosis at a tertiary care hospital in beijing, china: high prevalence of nonclustered healthcare-associated cases among adult patients. | listeriosis is an emerging infectious disease associated with high mortality. there are few published reports from east asia and developing countries. our goal was to describe the clinical characteristics and outcomes of patients diagnosed with listeria monocytogenes at a tertiary care hospital in beijing, china. | 2013 | 23175565 |
| epidemiology of infectious encephalitis, differences between a prospective study and hospital discharge data. | the french epidemiology of infectious encephalitis has been described in a 2007 prospective study. we compared these results with available data (demographic features, causative agents, case-fatality ratio) obtained through the french national hospital discharge 2007 database (pmsi), in order to evaluate it as a surveillance tool for encephalitis. causative agents were identified in 52% of cases in the study, and 38% in pmsi (p < 0·001). the incidence of encephalitis in france in 2007 was estima ... | 2013 | 23168268 |
| the changing pattern of bacterial meningitis in adult patients at a large tertiary university hospital in barcelona, spain (1982-2010). | we conducted a prospective, observational study in barcelona (spain) to determine changes in the spectrum of adult patients with bacterial meningitis (bm) over a 29-year period. | 2013 | 23168216 |