| growth of listeria monocytogenes in refrigerated ready-to-eat foods in japan. | the ability of l. monocytogenes to grow in a series of japanese ready-to-eat (rte) foods, including boiled baby sardine and japanese pickle, was tested at two different refrigeration temperatures. in rte foods in which l. monocytogenes can grow, growth was significantly higher at 10°c than that at 4°c during their shelf lives and growth patterns varied extensively among the different types of foods. however, growth did not occur at 4°c within the shelf life of certain rte foods, such as broiled ... | 2013 | 23697335 |
| mechanical and water barrier properties of agar/κ-carrageenan/konjac glucomannan ternary blend biohydrogel films. | multicomponent hydrogel films composed of agar, κ-carrageenan, konjac glucomannan powder, and nanoclay (cloisite(®) 30b) were prepared and their mechanical and water barrier properties such as water vapor permeability (wvp), water contact angle (ca), water solubility (ws), water uptake ratio (wur), water vapor uptake ratio (wvur) were determined. mechanical, water vapor barrier, and water resistance properties of the ternary blend film exhibited middle range of individual component films, howeve ... | 2013 | 23688456 |
| a differential fluorescence-based genetic screen identifies listeria monocytogenes determinants required for intracellular replication. | listeria monocytogenes is a gram-positive, facultative intracellular pathogen capable of causing severe invasive disease with high mortality rates in humans. while previous studies have largely elucidated the bacterial and host cell mechanisms necessary for invasion, vacuolar escape, and subsequent cell-to-cell spread, the l. monocytogenes factors required for rapid replication within the restrictive environment of the host cell cytosol are poorly understood. in this report, we describe a differ ... | 2013 | 23687268 |
| prevalence and quantification of listeria monocytogenes in chicken offal at the retail level in malaysia. | a total of 216 chicken offal samples (chicken liver = 72; chicken heart = 72; chicken gizzard = 72) from wet markets and hypermarkets in selangor, malaysia, were examined for the presence and density of listeria monocytogenes by using a combination of the most probable number and pcr method. the prevalence of l. monocytogenes in 216 chicken offal samples examined was 26.39%, and among the positive samples, the chicken gizzard showed the highest percentage at 33.33% compared with chicken liver (2 ... | 2013 | 23687164 |
| truncated form of tgf-βrii, but not its absence, induces memory cd8+ t cell expansion and lymphoproliferative disorder in mice. | inflammatory and anti-inflammatory cytokines play an important role in the generation of effector and memory cd8(+) t cells. we used two different models, transgenic expression of truncated (dominant negative) form of tgf-βrii (dntgfβrii) and cre-mediated deletion of the floxed tgf-βrii to examine the role of tgf-β signaling in the formation, function, and homeostatic proliferation of memory cd8(+) t cells. blocking tgf-β signaling in effector cd8(+) t cells using both of these models demonstrat ... | 2013 | 23686479 |
| prevalence of the lmo0036-0043 gene cluster encoding arginine deiminase and agmatine deiminase systems in listeria monocytogenes. | arginine deiminase and agmatine deiminase systems are involved in acid tolerance, and their encoding genes form the cluster lmo0036-0043 in listeria monocytogenes. while lmo0042 and lmo0043 were conserved in all l. monocytogenes strains, the lmo0036-0041 region of this cluster was identified in all lineages i and ii, and the majority of lineage iv (83.3%) strains, but absent in all lineage iii and a small fraction of lineage iv (16.7%) strains, suggesting that the presence of the complete lmo003 ... | 2013 | 23686125 |
| oral transmission of listeria monocytogenes in mice via ingestion of contaminated food. | l. monocytogenes are facultative intracellular bacterial pathogens that cause food borne infections in humans. very little is known about the gastrointestinal phase of listeriosis due to the lack of a small animal model that closely mimics human disease. this paper describes a novel mouse model for oral transmission of l. monocytogenes. using this model, mice fed l. monocytogenes-contaminated bread have a discrete phase of gastrointestinal infection, followed by varying degrees of systemic sprea ... | 2013 | 23685758 |
| initial adhesion of listeria monocytogenes to fine polished stainless steel under flow conditions is determined by prior growth conditions. | listeria monocytogenes is a food-borne pathogen known to persist in food production environments, where it is able to attach and form biofilms, potentially contaminating food products ready for consumption. in this study the first step in the establishment of l. monocytogenes in a food-processing environment was examined, namely the initial adhesion to stainless steel under specific dynamic flow conditions. it was found that the intrinsic ability of l. monocytogenes to adhere to solid surfaces u ... | 2013 | 23685728 |
| combined effect of benzalkonium chloride and ultrasound against listeria monocytogenes biofilm on plastic surface. | this study was performed to evaluate the effectiveness of benzalkonium chloride combined with ultrasound in eliminating listeria monocytogenes biofilm from polystyrene surface. the test strain of l. monocytogenes, previously classified as strong biofilm producer, was grown to form biofilm in tryptic soy broth at 20°c for 6 days in polystyrene specimen containers. the biofilms formed on surface of containers were treated with ultrasound alone, benzalkonium chloride (100 and 400 mg l(-1)) alone an ... | 2013 | 23682619 |
| cd11c(high)cd8+ regulatory t cell feedback inhibits cd4 t cell immune response via fas ligand-fas pathway. | regulatory t cells can restrict the uncontrolled immune response and inflammation, avoiding pathologic immune injury to the host and thus playing important roles in the maintenance of immune homeostasis. until recently, many subsets of cd4 and cd8 regulatory t cells have been reported. in this study, we identified cd11c(high)cd8(+) t cells as a new subset of cd8(+) regulatory t cells. during listeria monocytogenes and staphylococcus aureus infection, two subsets of cd8 t cells were classified ac ... | 2013 | 23677464 |
| characterization of two resuscitation promoting factors of listeria monocytogenes. | in actinobacteria, resuscitation promoting factor (rpf) proteins have been described as having the ability to increase the viable count of dormant cultures and stimulate growth of vegetative cells through lag phase reduction. recently, it was suggested that proteins lmo0186 and lmo2522 of listeria monocytogenes are equivalent to rpf proteins based on their genomic context and conserved domain architecture. it was proposed that they have evolved through non-orthologous displacement of the rpf dom ... | 2013 | 23676438 |
| critical roles of a dendritic cell subset expressing a chemokine receptor, xcr1. | dendritic cells (dcs) consist of various subsets that play crucial roles in linking innate and adaptive immunity. in the murine spleen, cd8α(+) dcs exhibit a propensity to ingest dying/dead cells, produce proinflammatory cytokines, and cross-present ags to generate cd8(+) t cell responses. to track and ablate cd8α(+) dcs in vivo, we generated xcr1-venus and xcr1-dtrvenus mice, in which genes for a fluorescent protein, venus, and a fusion protein consisting of diphtheria toxin receptor and venus ... | 2013 | 23670193 |
| crystal structures of bifunctional penicillin-binding protein 4 from listeria monocytogenes. | penicillin-binding proteins (pbps), which catalyze the biosynthesis of the peptidoglycan chain of the bacterial cell wall, are the major molecular target of bacterial antibiotics. here, we present the crystal structures of the bifunctional peptidoglycan glycosyltransferase (gt)/transpeptidase (tp) pbp4 from listeria monocytogenes in the apo-form and covalently linked to two β-lactam antibiotics, ampicillin and carbenicillin. the orientation of the tp domain with respect to the gt domain is disti ... | 2013 | 23669378 |
| survey of listeria monocytogenes, salmonella spp. and escherichia coli o157:h7 in raw ingredients and ready-to-eat products by commercial real-time pcr kits. | real-time pcr (rtipcr) assays including enrichment stage were evaluated for the rapid detection of listeria monocytogenes, salmonella spp. and escherichia coli o157:h7 in raw ingredients and ready-to-eat products using molecular beacon probes available as commercial kits (warnex genevision, canada & aes chemunex detection system, france). the accuracy of the assays was evaluated analyzing 1032 naturally contaminated food samples in combination to the conventional cultural methods. presence/absen ... | 2013 | 23664258 |
| live-attenuated listeria-based immunotherapy. | for decades listeria monocytogenes has been used as a model of host-disease immunology, and a considerable body of knowledge has been amassed regarding the complex immune response to l. monocytogenes. attenuated strains of l. monocytogenes are currently being assessed as therapeutic bacterial vectors to present tumor-associated antigens to the immune system for the clinical treatment of cancer. l. monocytogenes immunotherapy utilizes many synchronous and disparate action mechanisms that stimulat ... | 2013 | 23659298 |
| occurrence of mutations impairing sigma factor b (sigb) function upon inactivation of listeria monocytogenes genes encoding surface proteins. | bacteria of the genus listeria contain the largest family of lpxtg surface proteins covalently anchored to the peptidoglycan. the extent to which these proteins may function or be regulated cooperatively is at present unknown. because of their unique cellular location, we reasoned that distinct lpxtg proteins could act as elements contributing to cell wall homeostasis or influencing the stability of other surface proteins bound to peptidoglycan. to test this hypothesis, we used proteomics to ana ... | 2013 | 23657685 |
| age-dependent differences in systemic and cell-autonomous immunity to l. monocytogenes. | host defense against infection can broadly be categorized into systemic immunity and cell-autonomous immunity. systemic immunity is crucial for all multicellular organisms, increasing in importance with increasing cellular complexity of the host. the systemic immune response to listeria monocytogenes has been studied extensively in murine models; however, the clinical applicability of these findings to the human newborn remains incompletely understood. furthermore, the ability to control infecti ... | 2013 | 23653659 |
| prfa led to reduced biofilm formation and contributed to altered gene expression patterns in biofilm-forming listeria monocytogenes. | the foodborne pathogen listeria monocytogenes has the ability to develop biofilm in the food-processing environment, which becomes a major concern for food safety. prfa, a key transcriptional activator that regulates most of the known listerial virulence gene expression, has been shown to promote l. monocytogenes biofilm formation. in this study, the whole-genome microarray was used to identify differentially expressed genes associated with the putative interaction between biofilm formation and ... | 2013 | 23652633 |
| survival of listeria monocytogenes cells and the effect of extended frozen storage (-20°c) on the expression of its virulence gene. | listeria monocytogenes is the main bacteria incriminated in contaminating cold storage food and ready-to-eat products. survival of four l. monocytogenes food isolates was examined during freezing and cold storage. slices of artisanal cheese were inoculated with listeria strains and incubated at -20 °c. their survival rate was recorded during the storage period. then, transcript levels of four virulence genes (hlya, iap, fri, and flaa) were evaluated using a semiquantitative reverse transcriptase ... | 2013 | 23649307 |
| a comparative study of fine polished stainless steel, tin and tin/ag surfaces: adhesion and attachment strength of listeria monocytogenes as well as anti-listerial effect. | magnetron sputtering was used to produce nanocomposite tin and tin/ag coatings on stainless steel surfaces. the surface chemistry (edx), physicochemical properties (contact angles), topography and roughness parameters (wlp and afm) of the fine polished stainless steel (fpss), tin and tin/8.6 at.% ag surfaces were examined. real-time initial adhesion of two listeria monocytogenes strains (egde and 64) to the three surfaces was determined under flow conditions, and their attachment strength after ... | 2013 | 23643915 |
| growth potential of salmonella and listeria monocytogenes in ready-to-eat lettuce and collard greens packaged under modified atmosphere and in perforated film. | this study was aimed at determining the effects of different storage scenarios on the growth potential of salmonella strains and listeria monocytogenes in ready-to-eat (rte) mixes of iceberg and crisp lettuces (lactuca sativa) and collard greens (brassica oleracea). vegetables were submitted to minimal processing, experimentally contaminated to achieve 10(1) and 10(2) cfu/g, packed under modified atmosphere and in perforated film, and submitted to the following storage scenarios: i = 100 % of th ... | 2013 | 23643135 |
| implementation of statistical tools to support identification and management of persistent listeria monocytogenes contamination in smoked fish processing plants. | listeria monocytogenes persistence in food processing plants is a key source of postprocessing contamination of ready-to-eat foods. thus, identification and elimination of sites where l. monocytogenes persists (niches) is critical. two smoked fish processing plants were used as models to develop and implement environmental sampling plans (i) to identify persistent l. monocytogenes subtypes (ecori ribotypes) using two statistical approaches and (ii) to identify and eliminate likely l. monocytogen ... | 2013 | 23643121 |
| 4-1bb signal stimulates the activation, expansion, and effector functions of γδ t cells in mice and humans. | we show here that the expression of 4-1bb is rapidly induced in γδ t cells following antigenic stimulation in both mice and humans, and ligation of the newly acquired 4-1bb with an agonistic anti-4-1bb augments cell division and cytokine production. we further demonstrate that γδ rather than αβ t cells protect mice from listeria monocytogenes (lm) infection and 4-1bb stimulation enhances the γδ t-cell activities in the acute phase of lm infection. ifn-γ produced from γδ t cells was the major sol ... | 2013 | 23640752 |
| myeloid-derived suppressor cells have a central role in attenuated listeria monocytogenes-based immunotherapy against metastatic breast cancer in young and old mice. | myeloid-derived suppressor cells (mdscs) are present in large numbers in blood of mice and humans with cancer, and they strongly inhibit t-cell and natural killer (nk) cell responses, at young and old age. we found that a highly attenuated bacterium listeria monocytogenes (listeria(at))-infected mdsc and altered the immune-suppressing function of mdsc. | 2013 | 23640395 |
| effect of combination of essential oils and bacteriocins on the efficacy of gamma radiation against salmonella typhimurium and listeria monocytogenes. | using essential oils (eo) alone or combined eo with nisin to enhance the lethality of salmonella typhimurium and listeria monocytogenes to γ-irradiation treatment. | 2013 | 23631567 |
| molecular subtyping and virulence gene analysis of listeria monocytogenes isolates from food. | a total of 67 listeria monocytogenes isolates from 698 raw meat samples were characterized for molecular serogroup identification and antimicrobial susceptibility. approximately one third (32.8%) of the isolates belonged to molecular serogroup 1/2a, 3a, followed by 1/2c, 3c (26.9%), 1/2b, 3b, 7 (22.4%), 4b, 4d, 4e (16.4%) and 4a, 4c (1.5%). most of the l. monocytogenes isolates were susceptible to 14 antimicrobials tested but several were resistant to tetracycline, ciprofloxacin and nitrofuranto ... | 2013 | 23628615 |
| persistent listeria monocytogenes subtypes isolated from a smoked fish processing facility included both phage susceptible and resistant isolates. | contamination of ready-to-eat foods with listeria monocytogenes can typically be traced back to post-processing contamination from environmental sources; contamination is often linked to subtypes that persist in food associated environments. although phage-based biocontrol strategies have been proposed for controlling this pathogen, information on the efficacy of phage treatment against diverse l. monocytogenes subtypes from food associated environments is still limited. we identified subtypes t ... | 2013 | 23628613 |
| acid shock of listeria monocytogenes at low environmental temperatures induces prfa, epithelial cell invasion, and lethality towards caenorhabditis elegans. | the saprophytic pathogen listeria monocytogenes has to cope with a variety of acidic habitats during its life cycle. the impact of low-temperature coupled with ph decrease for global gene expression and subsequent virulence properties, however, has not been elucidated. | 2013 | 23622257 |
| the effect of ozone and open air factor on surface-attached and biofilm environmental listeria monocytogenes. | the effects of gaseous ozone and open air factor (oaf) on environmental listeria monocytogenes attached to three common food contact surfaces were investigated. | 2013 | 23621101 |
| refinement of the listeria monocytogenes σb regulon through quantitative proteomic analysis. | σ(b) is an alternative σ factor that regulates stress response and virulence genes in the foodborne pathogen listeria monocytogenes. to gain further insight into σ(b)-dependent regulatory mechanisms in l. monocytogenes, we (i) performed quantitative proteomic comparisons between the l. monocytogenes parent strain 10403s and an isogenic δsigb mutant and (ii) conducted a meta-analysis of published microarray studies on the 10403s σ(b) regulon. a total of 134 genes were found to be significantly po ... | 2013 | 23618998 |
| the effect of vaccines based on ovalbumin coupled to gas-filled microbubbles for reducing infection by ovalbumin-expressing listeria monocytogenes. | gas-filled microbubbles (mb) are a very promising alternative to the currently evaluated lipid- or polymer-based particulate ag delivery systems. we recently demonstrated the ability of mb to deliver associated ag to dc, to activate them and thereby induce both humoral and cellular immune responses. we now extended the characterization of mb as antigen-delivery system by appraising the efficiency of mb-associated ovalbumin (ova-mb) at protecting mice against pathogen infection. ultrasound-mediat ... | 2013 | 23618628 |
| influence of internalin a murinisation on host resistance to orally acquired listeriosis in mice. | the bacterial surface protein internalin (inla) is a major virulence factor of the food-born pathogen listeria monocytogenes. it plays a critical role in the bacteria crossing the host intestinal barrier by a species-specific interaction with the cell adhesion molecule e-cadherin. in mice, the interaction of inla with murine e-cadherin is impaired due to sequence-specific binding incompatibilities. we have previously used the approach of 'murinisation' to establish an oral listeriosis infection ... | 2013 | 23617550 |
| ovalbumin-related protein x is a heparin-binding ov-serpin exhibiting antimicrobial activities. | ovalbumin family contains three proteins with high sequence similarity: ovalbumin, ovalbumin-related protein y (ovay), and ovalbumin-related protein x (ovax). ovalbumin is the major egg white protein with still undefined function, whereas the biological activity of ovax and ovay has not yet been explored. similar to ovalbumin and ovay, ovax belongs to the ovalbumin serine protease inhibitor family (ov-serpin). we show that ovax is specifically expressed by the magnum tissue, which is responsible ... | 2013 | 23615912 |
| stereotactic biopsy and drainage of a brainstem abscess caused by listeria monocytogenes. | listerial rhombencephalitis and brain abscesses are rare, but potentially life-threatening conditions. early initiation of antibiotic therapy is crucial, but establishing the diagnosis of listerial brainstem abscess can be difficult. stereotactic biopsy and drainage of space-occupying abscesses of the brainstem should be considered especially in cases of rapid clinical deterioration. we successfully performed stereotactic biopsy and drainage of a listerial brainstem abscess in a 42-year-old male ... | 2013 | 23615421 |
| [effects of the deletion of rsbv gene on virulence of listeria monocytogenes]. | to study the effect of rsbv gene deletion on the virulence of listeria monocytogenes. | 2013 | 23614241 |
| evaluation of characteristics of pediococcus spp. to be used as a vaginal probiotic. | the aim of our research was to select, identify and characterize an isolate of lactic acid bacteria to be considered as a vaginal probiotic. | 2013 | 23611355 |
| nontoxic radioactive listeria(at) is a highly effective therapy against metastatic pancreatic cancer. | no significant improvement in therapy of pancreatic cancer has been reported over the last 25 y, underscoring the urgent need for new alternative therapies. here, we coupled a radioisotope, (188)rhenium, to an attenuated (at) live listeria monocytogenes (listeria(at)) using listeria-binding antibodies, thus creating a unique radioactive listeria(at) (rl). we then demonstrated in a highly metastatic pancreatic mouse tumor model (panc-02) that rl delivered radioactivity to the metastases and less ... | 2013 | 23610422 |
| virulence aspects of listeria monocytogenes lo28 high pressure-resistant variants. | high pressure treatment is a novel food processing approach for reducing pathogens in foods and food ingredients. however, relatively little is known about the pathogenic potential of organisms that survive the treatment. twelve previously isolated and characterized variants of listeria monocytogenes lo28 obtained after a high pressure treatment were assessed for their virulence potential and antibiotic susceptibility. ten variants showed attenuated virulence while two variants retained full vir ... | 2013 | 23603274 |
| listeriosis infection of an abdominal aortic aneurysm in a diabetic patient. | a rare case of an abdominal aortic aneurysm (aaa) infected by listeria monocytogenes in a 72-year-old male diabetic farmer, is reported. our patient had a history of a recent pneumonia that could have been caused by listeria too. aneurysm infection was manifested by fever and abdominal and back pain, which prompted investigation with ct scanning that revealed a 4.9 cm aaa with typical signs of infection. he underwent urgent aaa repair with aortobifemoral bypass grafting and had an uneventful cou ... | 2013 | 23599616 |
| the tat pathway is prevalent in listeria monocytogenes lineage ii and is not required for infection and spread in host cells. | listeria monocytogenes, a foodborne pathogenic bacterium, remains a serious public health concern due to its frequent occurrence in food products coupled with a high mortality rate. bacterial pathogenicity depends greatly on the ability to secrete virulence factors to or beyond the bacterial cell surface. the tat pathway, one of the secretion systems present in l. monocytogenes, was until now only investigated in silico. in l. monocytogenes strain egde two genes constitute this pathway, tatc(lmo ... | 2013 | 23595063 |
| weissellicin l, a novel bacteriocin from sian-sianzih-isolated weissella hellenica 4-7. | to identify and characterize novel bacteriocins from weissella hellenica 4-7. | 2013 | 23594122 |
| genomic and enzymatic results show bacillus cellulosilyticus uses a novel set of lpxta carbohydrases to hydrolyze polysaccharides. | alkaliphilic bacillus species are intrinsically interesting due to the bioenergetic problems posed by growth at high ph and high salt. three alkaline cellulases have been cloned, sequenced and expressed from bacillus cellulosilyticus n-4 (bcell) making it an excellent target for genomic sequencing and mining of biomass-degrading enzymes. | 2013 | 23593409 |
| listeria monocytogenes sequence type 6 and increased rate of unfavorable outcome in meningitis: epidemiologic cohort study. | we analyzed clinical characteristics, treatment, genetic diversity, and outcome of 92 adults with listeria monocytogenes meningitis included in 2 prospective nationwide cohort studies. | 2013 | 23592828 |
| predictive model for the reduction of heat resistance of listeria monocytogenes in ground beef by the combined effect of sodium chloride and apple polyphenols. | we investigated the combined effect of three internal temperatures (57.5, 60, and 62.5°c) and different concentrations (0 to 3.0 wt/wt.%) of sodium chloride (nacl) and apple polyphenols (app), individually and in combination, on the heat-resistance of a five-strain cocktail of listeria monocytogenes in ground beef. a complete factorial design (3×4×4) was used to assess the effects and interactions of heating temperature, nacl, and app. all 48 combinations were tested twice, to yield 96 survival ... | 2013 | 23587714 |
| an improved susceptibility test based on amberlite reveals the potential antilisterial activity of fosfomycin in vitro. | listeria monocytogenes is resistant to fosfomycin in vitro but is susceptible in vivo due to increased expression of positive regulator factor a (prfa) and its dependent factor, hexose phosphate transporter (hpt), upon infection of host cells. amberlite, a polymeric adsorbent resin, could induce prfa-dependent gene expression and thus, in theory, improve the sensitivity of l. monocytogenes to fosfomycin in vitro. in the current study, an improved susceptibility test based on amberlite was develo ... | 2013 | 23586749 |
| fibronectin-binding protein, fbpa, is the adhesin responsible for pathogenesis of listeria monocytogenes infection. | the role of fibronectin binding protein a (fbpa) in listeria monocytogenes infection and its pathogenesis were studied in vivo and in vitro by constructing a fbpa-deficient mutant of l. monocytogenes (δfbpa). in vivo, δfbpa was less pathogenic in mutant mice than was wild-type l. monocytogenes. fbpa did not affect the amounts of various virulence-determining factors, including internalin b and listeriolysin o. however, adherence to, and invasion of, mouse hepatocytes by the δfbpa mutant were red ... | 2013 | 23586629 |
| an internalin a probe-based genosensor for listeria monocytogenes detection and differentiation. | internalin a (inla), a protein required for listeria monocytogenes virulence, is encoded by the inla gene, which is only found in pathogenic strains of this genus. one of the best ways to detect and confirm the pathogenicity of the strain is the detection of one of the virulence factors produced by the microorganism. this paper focuses on the design of an electrochemical genosensor used to detect the inla gene in listeria strains without labelling the target dna. the electrochemical sensor was o ... | 2013 | 23586053 |
| host and bacterial factors that regulate lc3 recruitment to listeria monocytogenes during the early stages of macrophage infection. | listeria monocytogenes is a bacterial pathogen that can escape the phagosome and replicate in the cytosol of host cells during infection. we previously observed that a population (up to 35%) of l. monocytogenes strain 10403s colocalize with the macroautophagy marker lc3 at 1 h postinfection. this is thought to give rise to spacious listeria-containing phagosomes (slaps), a membrane-bound compartment harboring slow-growing bacteria that is associated with persistent infection. here, we examined t ... | 2013 | 23584039 |
| optimized multilocus variable-number tandem-repeat analysis assay and its complementarity with pulsed-field gel electrophoresis and multilocus sequence typing for listeria monocytogenes clone identification and surveillance. | populations of the food-borne pathogen listeria monocytogenes are genetically structured into a small number of major clonal groups, some of which have been implicated in multiple outbreaks. the goal of this study was to develop and evaluate an optimized multilocus variable number of tandem repeat (vntr) analysis (mlva) subtyping scheme for strain discrimination and clonal group identification. we evaluated 18 vntr loci and combined the 11 best ones into two multiplexed pcr assays (mlva-11). a c ... | 2013 | 23576539 |
| diagnostic exercise: suppurative rhombencephalitis and meningitis in a goat. | a 1-year-old female boer goat was presented for necropsy following spontaneous death and history of acute recumbency, nystagmus, and sialorrhea. a swollen area was grossly observed in the brainstem at the level of the pons. on cut surface, the right cerebellar peduncles were expanded by a focal, pale, poorly demarcated, slightly depressed, and soft area of malacia. microscopically, this area contained diffuse edema and necrosis, with microabscesses, neuronal necrosis, neuronophagia, axonal spher ... | 2014 | 23576239 |
| incidence of listeria monocytogenes and listeria spp. in a small-scale mushroom production facility. | listeria monocytogenes is a foodborne pathogen of significant concern to the agricultural and food processing industry because of its ability to grow and persist in cool and moist environments and its association with listeriosis, a disease with a very high mortality rate. although there have been no listeriosis outbreaks attributed to fresh mushrooms in the united states, retail surveys and recalls are evidence that l. monocytogenes contamination of mushrooms (agaricus bisporus) can occur. the ... | 2013 | 23575122 |
| listeriosis in patients receiving biologic therapies. | the evolution of inflammatory diseases has radically changed since the introduction of biologic therapies, such as tumour necrosis factor alpha inhibitors (anti-tnfα). they, therefore, represent a widely used therapeutic modality. nevertheless, post-marketing studies reveal an increased risk of infection in patients taking these drugs, especially granulomatous infections such as listeriosis. we aimed to evaluate the reported cases of listeriosis in patients treated with biologic treatments. we u ... | 2013 | 23568606 |
| nation-wide study of the occurrence of listeria monocytogenes in french soils using culture-based and molecular detection methods. | soil is a potential reservoir of human pathogens and a possible source of contamination of animals, crops and water. in order to study the distribution of listeria monocytogenes in french soils, a real-time pcr taqman assay targeting the phosphoribosylpyrophosphate synthetase (prs) gene of l. monocytogenes was developed for the specific detection and quantification of this bacterium within a collection of 1315 soil dnas originated from the french soil quality monitoring network. the prs real-tim ... | 2013 | 23566826 |
| mutations in gltb and gltc reduce oxidative stress tolerance and biofilm formation in listeria monocytogenes 4b g. | the foodborne pathogen listeria monocytogenes has the capability to persist on surfaces in food-processing environments, and the organism is resistant to environmental stresses. in this study, a tn917 insertion mutant of l. monocytogenes 4b g showing reduced biofilm formation and sensitivity to oxidative stress was identified and characterized. the transposon insertion site within the gltb gene was identified by inverse pcr. the gltc gene is located upstream and is reported to be transcribed div ... | 2013 | 23562858 |
| molluskan fasciclin-1 domain-containing protein: molecular characterizationand gene expression analysis of fasciclin 1-like protein from disk abalone (haliotis discus discus). | cell-to-cell contacts play a key role in multicellular systems and organisms. fasciclin-1 (fas-1) is a lipid-linked membrane associated glycoprotein that is a member of a newly recognized family of cell adhesion molecules sharing features with the immunoglobulins, cadherins, integrins, and selectins. here, we report the identification and molecular characterization of a novel fas-1 domain-containing cdna from disk abalone (haliotis discus discus), including its gene expression profile and immune ... | 2013 | 23562785 |
| ligands affecting silver antimicrobial efficacy on listeria monocytogenes and salmonella enterica. | although silver is being extensively used in food or other applications as the key component to control microbial proliferation, many factors affecting its real potential are still unknown. in the present work, the presence of specific ligands or the contents in organic matter was correlated with silver speciation and its antibacterial performance. silver was found to be only active in form of free silver ions (fsi). the presence of chloride ions produced an equilibrium of stable silver chloride ... | 2013 | 23561107 |
| effect of a combination of low level ozone and metal ions on reducing escherichia coli o157:h7 and listeria monocytogenes. | ozonated water has been used as a strong antimicrobial agent against foodborne pathogens. in this study, the combined effect of low level ozonated water and different added components, including 0.2% starch and metal ions (1 mm cucl2·2h2o and 0.1 mm agno3), on inactivation of escherichia coli o157:h7 and listeria monocytogenes was investigated. treatment with 0.4 ppm ozonated water for 30 min resulted in a maximum log reduction in e. coli o157:h7 and l. monocytogenes compared to initial bacteria ... | 2013 | 23558542 |
| inactivation of listeria monocytogenes on frankfurters by plant-derived antimicrobials alone or in combination with hydrogen peroxide. | listeria monocytogenes is a significant foodborne pathogen associated with outbreaks involving contaminated ready-to-eat (rte) products, including frankfurters. the usda-fsis has established a zero tolerance policy for l. monocytogenes in rte products, thereby warranting effective post-processing interventions to control the pathogen on these foods. in the present study, the antilisterial activity of gras (generally recognized as safe)-status plant-derived antimicrobials (pdas), namely β-resorcy ... | 2013 | 23558194 |
| listeria monocytogenes dairy isolates show a different proteome response to sequential exposure to gastric and intestinal fluids. | the gastrointestinal system poses different stresses to the foodborne pathogen, listeria monocytogenes, including the low ph of the stomach and the presence of bile and the high osmolality of the intestinal fluid. the present study evaluated how previous exposure of three l. monocytogenes dairy isolates (c882 and t8, serovar 4b isolates and a9 serovar 1/2a or 3b isolate) to a cheese-simulated medium (p h5.5 and 3.5% nacl [w/v], adapted cultures) affected subsequent survival in a simulated gastro ... | 2013 | 23558187 |
| connexin43 is dispensable for phagocytosis. | macrophages that lack connexin43 (cx43), a gap junction protein, have been reported to exhibit dramatic deficiencies in phagocytosis. in this study, we revisit these findings using well-characterized macrophage populations. cx43 knockout (cx43(-/-)) mice die soon after birth, making the harvest of macrophages from adult cx43(-/-) mice problematic. to overcome this obstacle, we used several strategies: mice heterozygous for the deletion of cx43 were crossed to produce cx43(+/+) (wild type [wt]) a ... | 2013 | 23554311 |
| developing the potential of using galleria mellonella larvae as models for studying brain infection by listeria monocytogenes. | | 2013 | 23552811 |
| listeria monocytogenes pleuritis: report of a geriatric case. | | 2013 | 23551348 |
| cd30l/cd30 is critical for maintenance of il-17a-producing γδ t cells bearing vγ6 in mucosa-associated tissues in mice. | cd30 ligand (cd30l, cd153), a member of the tumor necrosis factor (tnf) superfamily, and its receptor cd30 are important for differentiation and activation of cd4(+) t helper type 17 (th17) cells. in this report, we demonstrate that the interleukin 17a (il-17a)-producing γδ t cells normally developed in the fetal thymus, whereas vγ1(-)vγ4(-) γδ t cells expressed vγ6/vδ1 gene transcript selectively decreased in mucosa-associated tissues in naive cd30ko or cd30lko mice. moreover, cd30 and cd30l we ... | 2013 | 23549449 |
| modeling the growth of listeria monocytogenes in mold-ripened cheeses. | this study presents possible applications of predictive microbiology to model the safety of mold-ripened cheeses with respect to bacteria of the species listeria monocytogenes during (1) the ripening of camembert cheese, (2) cold storage of camembert cheese at temperatures ranging from 3 to 15°c, and (3) cold storage of blue cheese at temperatures ranging from 3 to 15°c. the primary models used in this study, such as the baranyi model and modified gompertz function, were fitted to growth curves. ... | 2013 | 23548297 |
| physical-mechanical and antimicrobial properties of nanocomposite films with pediocin and zno nanoparticles. | this work aimed to develop nanocomposite films of methyl cellulose (mc) incorporated with pediocin and zinc oxide nanoparticles (nanozno) using the central composite design and response surface methodology. this study evaluated film physical-mechanical properties, including crystallography by x-ray diffraction, mechanical resistance, swelling and color properties, microscopy characterization, thermal stability, as well as antimicrobial activity against staphylococcus aureus and listeria monocyto ... | 2013 | 23544529 |
| magnetic nano-beads based separation combined with propidium monoazide treatment and multiplex pcr assay for simultaneous detection of viable salmonella typhimurium, escherichia coli o157:h7 and listeria monocytogenes in food products. | we developed a rapid and reliable technique for simultaneous detection of salmonella typhimurium, escherichia coli o157:h7 and listeria monocytogenes that can be used in food products. magnetic nano-beads (mnbs) based immunomagnetic separation (ims) was used to separate the target bacterial cells while multiplex pcr (mpcr) was used to amplify the target genes. to detect only the viable bacteria, propidium monoazide (pma) was applied to selectively suppress the dna detection from dead cells. the ... | 2013 | 23541211 |
| antimicrobial resistance and co-selection phenomenon in listeria spp. recovered from food and food production environments. | antimicrobial resistance (amr), co-selection phenomenon, and the relationship between reduced susceptibility (rsc) to ciprofloxacin (cip) and resistance to other antimicrobials in listeria spp. (n = 103) recovered from food processing environments (fpe) and food were investigated. resistance of listeria monocytogenes and other listeriae, respectively, to cefoxitin (fox; 98% vs. 88%), cip (7% vs. 4%), clindamycin (cli; 33% vs. 59%) and tetracycline (6% vs. 8%) was observed, as was rsc to cip (67% ... | 2013 | 23541198 |
| the application of high-concentration short-time chlorine dioxide treatment for selected specialty crops including roma tomatoes (lycopersicon esculentum), cantaloupes (cucumis melo ssp. melo var. cantaloupensis) and strawberries (fragaria×ananassa). | the effects of high-concentration short-time chlorine dioxide (clo2) gas treatment on food-borne pathogens inoculated onto the surface of tomatoes, cantaloupes, and strawberries were studied. produce were spot-inoculated with a mixture of salmonella enterica (serotypes montevideo, javiana and baildon), escherichia coli o157:h7 (serotypes 204 p, edl 933 and c792) or listeria monocytogenes (serotypes scott a, f 5069 and lcdc 81-861), and treated with clo2 gas at 10 mg/l for 180 s. after clo2 gas t ... | 2013 | 23541196 |
| combination use of pfge and drug-resistant analysis in the epidemiological investigation of listeria monocytogenes. | | 2013 | 23534473 |
| [cerebral listeria abscess in a patient with gastric cancer]. | history and presentation on admission: a 70-year-old woman with a history of gastric cancer and palliative chemotherapy was admitted with disturbed consciousness. | 2013 | 23533043 |
| comparative analysis of the exoproteomes of listeria monocytogenes strains grown at low temperatures. | this article presents the major differences in the exoproteomes of listeria monocytogenes strains grown at 11°c and 20°c, and their comparison to 37°c, the optimal temperature of growth of this foodborne pathogenic bacteria. a set of four strains previously characterized and representing the genetic diversity of the species was used. two were virulent, of which one was persistent, and two were low virulent strains. the proteins secreted by the strains grown in minimal medium were separated by so ... | 2013 | 23531123 |
| listeria monocytogenes arca contributes to acid tolerance. | the foodborne pathogen listeria monocytogenes is able to colonize the human and animal intestinal tracts and subsequently crosses the intestinal barrier, causing systemic infection. for successful establishment of infection, l. monocytogenes must survive and adapt to the low ph environment of the stomach. gene sequence analysis indicates that lmo0043, an orthologue of arca, encodes a protein containing conserved motifs and critical active amino acids characteristic of arginine deiminase that med ... | 2013 | 23518652 |
| cross-recognition of aspergillus galactomannan caused by listeria monocytogenes infection. | we report a case of listeria monocytogenes bacteremia in a leukemic patient having a positive assay for aspergillus galactomannan (gm), although no evidence of aspergillosis was found. supernatant obtained from l. monocytogenes strain suspension was reactive with gm-assay. l. monocytogenes produces a soluble antigen that is cross-reactive with aspergillus gm. | 2013 | 23518185 |
| fas-mediated inflammatory response in listeria monocytogenes infection. | the molecular mechanisms of fas (cd95/apo-1)-mediated apoptosis are increasingly understood. however, the role of fas-mediated production of proinflammatory cytokines such as il-18 and il-1β in bacterial infection is unclear. we demonstrate the importance of fas-mediated signaling in il-18/il-1β production postinfection with listeria monocytogenes without the contribution of caspase-1 inflammasome. il-18/il-1β production in l. monocytogenes-infected peritoneal exudate cells from fas-deficient mi ... | 2013 | 23509366 |
| structural and dynamic features of cold-shock proteins of listeria monocytogenes, a psychrophilic bacterium. | cold-shock proteins (csps), proteins expressed when the ambient temperature drops below the growth-supporting temperature, bind to single-stranded nucleic acids and act as rna chaperones to regulate translation. listeria monocytogenes is a psychrophilic food-borne pathogen that is problematic for the food industry. structures of csps from psychrophilic bacteria have not yet been studied. despite dramatic differences in the thermostability of csps of various thermophilic microorganisms, these pro ... | 2013 | 23506337 |
| cellular-flip, raji isoform (c-flip r) modulates cell death induction upon t-cell activation and infection. | dysregulation of apoptosis caused by an imbalance of pro- and anti-apoptotic protein expression can lead to cancer, neurodegenerative, and autoimmune diseases. cellular-flip (c-flip) proteins inhibit apoptosis directly at the death-inducing signaling complex of death receptors, such as cd95, and have been linked to apoptosis regulation during immune responses. while the isoforms c-flipl and c-flips are well characterized, the function of c-flipr remains poorly understood. here, we demonstrate th ... | 2013 | 23505065 |
| gene expression profiling of a nisin-sensitive listeria monocytogenes scott a ctsr deletion mutant. | listeria monocytogenes is a food-borne pathogen of significant threat to public health. nisin is the only bacteriocin that can be used as a food preservative. due to its antimicrobial activity, it can be used to control l. monocytogenes in food; however, the antimicrobial mechanism of nisin activity against l. monocytogenes is not fully understood. the ctsr (class iii stress gene repressor) protein negatively regulates the expression of class iii heat shock genes. a spontaneous pressure-tolerant ... | 2013 | 23494707 |
| susceptibility of aged guinea pigs to repeated daily challenge with listeria monocytogenes. | epidemiological data suggests that certain groups such as the elderly are more susceptible to listeriosis than the general population. repeated exposure to low levels of listeria monocytogenes may increase the probability of infection. increased susceptibility to infection in the elderly has been attributed in part to reduced activity of t cells. we investigated the impact of consecutive daily intragastric challenge with l. monocytogenes on the development of listeriosis within an aged guinea pi ... | 2013 | 23489051 |
| rapid and sensitive immunodetection of listeria monocytogenes in milk using a novel piezoelectric cantilever sensor. | listeria monocytogenes (lm), an important food-borne pathogen that has a high mortality rate (≈ 30%), was successfully detected within an hour at the infection dose limit of 10(3)/ml, both in buffer and milk. lm detection was demonstrated using a novel asymmetrically anchored cantilever sensor and a commercially available antibody. sensor responses were confirmed using a secondary antibody binding step, similar to the sandwich elisa assays, as a means of signal amplification that also reduced th ... | 2013 | 23475140 |
| preservative action of 11s (glycinin) and 7s (β-conglycinin) soy globulin on bovine raw milk stored either at 4 or 25 °c. | considerable inhibitory antibacterial actions were exerted by the soybean 11s subunit comparable with nisin on the proliferation of total viable count, pseudomonas count and enterobacteriaceae count in bovine milk stored at 4 or 25 °c for 30 d and 48 h, while 7s and lysozyme were much less effective. the maximum magnitudes of bacterial reduction by 11s and nisin were in the range 2-4 log cfu/ml. the proliferation of 3 pathogenic bacteria (listeria monocytogenes, salmonella enteritidis and escher ... | 2013 | 23473420 |
| the central role of arginine catabolism in t-cell dysfunction and increased susceptibility to infection after physical injury. | to explore the hypothesis that decreased arginine availability by myeloid-derived suppressor cells (mdscs) is a cause of t-cell dysfunction after physical injury (pi). | 2014 | 23470573 |
| successful vaccination of immune suppressed recipients using listeria vector hiv-1 vaccines in helminth infected mice. | vaccines for hiv, malaria and tb remain high priorities, especially for sub-saharan populations. the question is: will vaccines currently in development for these diseases function in populations that have a high prevalence of helminth infection? infection with helminth parasites causes immune suppression and a cd4+ th2 skewing of the immune system, thereby impairing th1-type vaccine efficacy. in this study, we conduct hiv vaccine trials in mice with and without chronic helminth infection to mim ... | 2013 | 23470236 |
| antimicrobial efficacy of poly (dl-lactide-co-glycolide) (plga) nanoparticles with entrapped cinnamon bark extract against listeria monocytogenes and salmonella typhimurium. | nanoencapsulation of active compounds using poly-(d,l-lactide-co-glycolide) (plga) is commonly used in the pharmaceutical industry for drug delivery and may have important applications in the food industry. control of growth of foodborne bacteria with the goals of reducing the number of foodborne illness outbreaks, assuring consumers a safer food supply remains a priority in the food industry. natural antimicrobials are an excellent way to eliminate pathogens without introducing chemical preserv ... | 2013 | 23464835 |
| antimicrobial activity of nanodispersed thymol in tryptic soy broth. | food safety is a continuing challenge for the food industry due to sporadic illness outbreaks caused by foodborne pathogens. plant essential oils have been studied extensively as natural antimicrobials to control foodborne pathogens. however, their hydrophobic nature makes application in foods difficult because of their low water solubility, adverse impact on sensory quality, and binding with food components, which can interfere with antimicrobial efficacy. the objective of this study was to cha ... | 2013 | 23462081 |
| frequency of salmonella and listeria monocytogenes in five commercial brands of chicken eggs using a combined method of enrichment and nested-pcr. | eggs or egg-based foods, either raw or undercooked, have been identified as vehicles of salmonella outbreaks. the low numbers of salmonella organisms in eggs makes it difficult to detect them in frequency studies. the nested-pcr (n-pcr) technique shows more sensitivity and specificity than bacteriological culture methods (bcms). a preenrichment method followed by enrichment and n-pcr is a good alternative for the investigation of salmonella and listeria monocytogenes in eggs. a total of 2,650 ch ... | 2013 | 23462079 |
| commercial thermal process for inactivating salmonella poona on surfaces of whole fresh cantaloupes. | outbreaks of salmonellosis by salmonella poona and listeriosis by listeria monocytogenes have been associated with the consumption of cantaloupes. commercial washing processes for cantaloupes are limited in their ability to inactivate and/or remove this human pathogen. our objective was to develop a commercial-scale surface pasteurization process for enhancing microbiological safety of cantaloupes. whole cantaloupes, surface inoculated with salmonella poona rm 2350, were stored at 32°c for 24 h ... | 2013 | 23462078 |
| fda-irisk--a comparative risk assessment system for evaluating and ranking food-hazard pairs: case studies on microbial hazards. | stakeholders in the system of food safety, in particular federal agencies, need evidence-based, transparent, and rigorous approaches to estimate and compare the risk of foodborne illness from microbial and chemical hazards and the public health impact of interventions. fda-irisk (referred to here as irisk), a web-based quantitative risk assessment system, was developed to meet this need. the modeling tool enables users to assess, compare, and rank the risks posed by multiple food-hazard pairs at ... | 2013 | 23462073 |
| adaptation of listeria monocytogenes in a simulated cheese medium: effects on virulence using the galleria mellonella infection model. | the aim of this study was to evaluate the effect of the acid and salt adaptation in a cheese-based medium on the virulence potential of listeria monocytogenes strains isolated from cheese and dairy processing environment using the galleria mellonella model. four l. monocytogenes strains were exposed to a cheese-based medium in conditions of induction of an acid tolerance response and osmotolerance response (ph 5·5 and 3·5% w/v nacl) and injected in g. mellonella insects. the survival of insects ... | 2013 | 23461530 |
| listeria monocytogenes alters mast cell phenotype, mediator and osteopontin secretion in a listeriolysin-dependent manner. | whilst mast cells participate in the immune defence against the intracellular bacterium listeria monocytogenes, there is conflicting evidence regarding the ability of l. monocytogenes to infect mast cells. it is known that the pore-forming toxin listeriolysin (llo) is important for mast cell activation, degranulation and the release of pro-inflammatory cytokines. mast cells, however, are a potential source of a wide range of cytokines, chemokines and other mediators including osteopontin, which ... | 2013 | 23460827 |
| different assembly of acid and salt tolerance response in two dairy listeria monocytogenes wild strains. | a lack on the association between acid tolerance response (atr) and osmotolerance response (otr) among listeria monocytogenes dairy isolates was found. in order to evaluate how wild l. monocytogenes isolates mount tolerance responses under a sub-lethal ph and a low sodium chloride concentration (ph 5.5 and 3.5 % [w/v] nacl), a proteomic approach was used. the atr and otr of two l. monocytogenes cheese dairy isolates (strain t8, serotype 4b and a9, serotype 1/2b or 3b) were determined. the proteo ... | 2013 | 23455450 |
| ruminant rhombencephalitis-associated listeria monocytogenes strains constitute a genetically homogeneous group related to human outbreak strains. | listeriosis is a disease that causes significant economic losses at the farm level because of high morbidity and mortality in ruminants. this study was performed to investigate the role of ruminants in the epidemiology of listeriosis in northern italy and the possible association of animal-adapted strains of listeria monocytogenes with strains associated with human disease. twenty ruminant rhombencephalitis isolates previously confirmed as l. monocytogenes by bacteriology and pcr were characteri ... | 2013 | 23455337 |
| characterizing uncertainty when evaluating risk management metrics: risk assessment modeling of listeria monocytogenes contamination in ready-to-eat deli meats. | this report illustrates how the uncertainty about food safety metrics may influence the selection of a performance objective (po). to accomplish this goal, we developed a model concerning listeria monocytogenes in ready-to-eat (rte) deli meats. this application used a second order monte carlo model that simulates l. monocytogenes concentrations through a series of steps: the food-processing establishment, transport, retail, the consumer's home and consumption. the model accounted for growth inhi ... | 2013 | 23454818 |
| survival of listeria monocytogenes and salmonella enteritidis in sea bream (sparus aurata) fillets packaged under enriched co2 modified atmospheres. | the effect of packaging of fresh sea bream fillets under different modified atmospheres (high levels of co2 combined with n2) on the behaviour of listeria monocytogenes and salmonella enteritidis was studied. two different strains of each pathogen were spread over the sea bream fillets stored at either 0 °c or 4 °c for 16 days under standard supermarket lighting conditions (14 h a day). the atmospheres investigated were 60% co2/40% n2, 70% co2/30% n2, 80% co2/20% n2 and air as control. mesophili ... | 2013 | 23454810 |
| peptide-bacteria interactions using engineered surface-immobilized peptides from class iia bacteriocins. | specificity of the class iia bacteriocin leucocin a (leua), an antimicrobial peptide active against gram-positive bacteria, including listeria monocytogenes , is known to be dictated by the c-terminal amphipathic helical region, including the extended hairpin-like structure. however, its specificity when attached to a substrate has not been investigated. exploiting properties of leua, we have synthesized two leua derivatives, which span the amphipathic helical region of the wild-type leua, consi ... | 2013 | 23445325 |
| fate of mycobacterium tuberculosis in peroxidase-loaded resting murine macrophages. | myeloperoxidase (mpo), in the presence of hydrogen peroxide and a halide represent an efficient microbicidal mechanism of phagocytic cells. mpo is abundant in neutrophils which also respond to infection by producing large amounts of reactive oxygen species (ros). mpo, ros and halide constitute a very toxic antimicrobial system (called the klebanoff system or ks). resting mature macrophages do not contain granular mpo and thus are unable to kill pathogenic mycobacteria and some other microorganis ... | 2013 | 26785781 |
| proteomic analysis shows that individual listeria monocytogenes strains use different strategies in response to gastric stress. | ingestion of contaminated dairy products, in particular soft cheese, is one of the major routes of infection by the human pathogen listeria monocytogenes. during cheese processing, this foodborne pathogen is exposed to sublethal acid and osmotic stress conditions, which may induce tolerance responses and influence subsequent survival in the gastric tract. the aim of the current study was to evaluate the impact on a l. monocytogenes cheese isolate (serotype 4b) and two cheese dairy isolates (t8, ... | 2013 | 23441912 |
| genome sequencing identifies two nearly unchanged strains of persistent listeria monocytogenes isolated at two different fish processing plants sampled 6 years apart. | listeria monocytogenes is a food-borne human-pathogenic bacterium that can cause infections with a high mortality rate. it has a remarkable ability to persist in food processing facilities. here we report the genome sequences for two l. monocytogenes strains (n53-1 and la111) that were isolated 6 years apart from two different danish fish processers. both strains are of serotype 1/2a and belong to a highly persistent dna subtype (random amplified polymorphic dna [rapd] type 9). we demonstrate us ... | 2013 | 23435887 |
| food microbe tracker: a web-based tool for storage and comparison of food-associated microbes. | large amounts of molecular subtyping information are generated by the private sector, academia, and government agencies. however, use of subtype data is limited by a lack of effective data storage and sharing mechanisms that allow comparison of subtype data from multiple sources. currently available subtype databases are generally limited in scope to a few data types (e.g., mlst. net) or are not publicly available (e.g., pulsenet). we describe the development and initial implementation of food m ... | 2013 | 23433376 |
| tracking cross-contamination transfer dynamics at a mock retail deli market using glogerm. | ready-to-eat (rte) deli meats are considered a food at high risk for causing foodborne illness. deli meats are listed as the highest risk rte food vehicle for listeria monocytogenes. cross-contamination in the retail deli market may contribute to spread of pathogens to deli meats. understanding potential cross-contamination pathways is essential for reducing the risk of contaminating various products. the objective of this study was to track cross-contamination pathways through a mock retail del ... | 2013 | 23433375 |
| prevalence and characteristics of listeria monocytogenes in bovine colostrum in japan. | this study was conducted to determine the prevalence and characteristics of listeria monocytogenes in bovine colostrum in japan. we collected bovine colostrum samples from 210 dams from 21 dairy farms in hokkaido prefecture (japan) between march and june 2009. l. monocytogenes was detected in samples from 6 (28.6%) of the 21 farms. of the 210 samples, 16 (7.6%) were positive for l. monocytogenes. we recovered 80 l. monocytogenes isolates; 44 (55%) isolates were classified as serotype 1/2b and 36 ... | 2013 | 23433372 |