| polyarteritis nodosa and extrahepatic manifestations of hbv infection: the case against autoimmune intervention in pathogenesis. | numerous extrahepatic manifestations have been reported in patients with both acute and chronic hepatitis b (arthralgias or arthritis, skin rashes, glomerulonephritis and neuritis), all of which are present in polyarteritis nodosa (pan) which is the most unique and spectacular extrahepatic manifestation. in the 1970s, the frequency of pan due to the hepatitis b (hbv) reached 30%. immunization programs explain the decrease and it is now down to 7%. pan usually occurs within 6 months of infection. ... | 2001 | 11334492 |
| [congenital aplastic anemia caused by parvovirus b19 infection]. | human parvovirus b19 causes the fifth disease (erythema infectiosum) in childhood. intrauterine infection by parvovirus in immunocompromised fetus can lead to the severe congenital aplastic anemia. here we report the case of 2.5-year-old girl with congenital infection with parvovirus b19. the girl was born as a pre-term baby with hydrops, congenital aplastic anemia, and low level of immunoglobulins igg and igm. she depended on the repeated blood transfusions and she did not respond to erythropoi ... | 2001 | 11347208 |
| treatment of parvovirus b19-associated polyarteritis nodosa with intravenous immune globulin. | | 2001 | 11357850 |
| out of sight, but not out of mind. | clinicians have had some success in treating or preventing several rarely discussed opportunistic infections. the author discusses seven infections, outlining the disease and possible treatments. aspergillosis, a fungal infection found in the lungs and sinuses, can be treated with intravenous amphotericin b. however, researchers are studying oral itraconazole as an alternative treatment. b-19 parvovirus is a viral infection that may cause severe anemia, a decrease in red blood cell count or hemo ... | 1995 | 11362370 |
| cellular transcription factors that interact with p6 promoter elements of parvovirus b19. | all transcripts of the human parvovirus b19 identified so far are regulated by a single promoter at map unit 6 of the viral genome, the so-called p6 promoter. this promoter is active in a wide variety of different cells. in order to identify cellular transcription factors involved in regulating promoter activity, we performed gel-retardation and supershift assays using the parts of the p6 promoter sequence shown previously to be protected in footprint experiments. thereby, binding was demonstrat ... | 2001 | 11369893 |
| quantitation of viral dna by real-time pcr applying duplex amplification, internal standardization, and two-color fluorescence detection. | a real-time pcr method was developed to quantitate viral dna that includes duplex amplification, internal standardization, and two-color fluorescence detection without the need to generate an external standardization curve. applied to human parvovirus b19 dna, the linear range was from 10(2) to at least 5 x 10(6) copies per ml of sample. the coefficient of variation was 0.29 using a run control of 2,876 copies per ml. the method reduces the risk of false-negative results, yields high precision, ... | 2001 | 11375203 |
| monoclonal anti-cd52 antibodies: a potential mode of therapy for parvovirus b(19) hepatitis. | | 2001 | 11377483 |
| frequency of human parvovirus b19 infection in intrauterine fetal death. | parvovirus b19 is known to cause fetal death in the second trimester, mainly in combination with hydrops fetalis. however, the frequency of parvovirus-b19-associated non-hydropic fetal loss in the late second and third trimester has not been thoroughly investigated. we aimed to investigate the frequency of parvovirus b19 infection in unselected cases of intrauterine fetal death and to assess the sensitivity of different diagnostic procedures. | 2001 | 11377602 |
| association of parvovirus b19 infection with idiopathic collapsing glomerulopathy. | collapsing glomerulopathy (cg), a disorder with severe glomerular and tubular involvement, occurs either as an idiopathic lesion or in some patients with human immunodeficiency virus (hiv) infection known as hiv-associated nephropathy (hivan). we previously reported a renal transplant recipient with de novo cg and red cell aplasia in association with persistent parvovirus b19 (pvb19) infection. this prompted us to look for an association between pvb19 infection and cg. | 2001 | 11380814 |
| [scapula alata as a complication of infection with human parvovirus b19 (erythema infectiosum)]. | this article reports a case of a 38-year-old woman suffering from erythema infectiosum (slap-cheek disease) complicated by arthritis and severe brachial plexus neuritis resulting in scapula winging. | 2001 | 11386110 |
| anti-vp1 and anti-vp2 antibodies detected by immunofluorescence assays in patients with acute human parvovirus b19 infection. | acute human parvovirus b19 infection is followed by an antibody response to the structural proteins of the viral capsid (vp1 and vp2). we used 80 sera collected from 58 erythema infectiosum and 6 transient aplastic crisis patients to test igm and igg antibodies against these two proteins in an immunofluorescence assay (ifa) using sf9 cells infected with recombinant baculovirus expressing either vp1 or vp2 antigen. although less sensitive than igm capture enzyme immunoassay using native antigen ( ... | 2001 | 11391423 |
| identification of an immunodominant peptide in the parvovirus b19 vp1 unique region able to elicit a long-lasting immune response in humans. | the immune response against parvovirus b19 is mainly directed against the two structural proteins, vp1 and vp2. the amino terminal half of the vp1 unique region has been shown to elicit a dominant immune response in humans, more effective than other linear epitopes and also it has been seen to contain significant neutralizing linear epitopes. three overlapping recombinant peptides corresponding to amino acids 2-40 (vp1-a), amino acids 32-71 (vp1-b), and amino acids 60-100 (vp1-c) of the vp1 uniq ... | 2001 | 11398810 |
| seroprevalence of known and putative hepatitis markers in united states blood donors with alt levels at least 120 iu per l. | alt testing of blood donors was initiated as a surrogate marker for non-a, non-b hepatitis. increased sensitivity of subsequent hbv and hcv tests used for standard donor screening make any residual value of alt testing questionable. | 2001 | 11399814 |
| [generalized petechial exanthema]. | | 2001 | 11412413 |
| baculovirus expression of parvovirus b19 (b19v) ns1: utility in confirming recent infection. | the presence of anti-parvovirus b19 (b19v) igm against viral capsid proteins (vp1 and vp2) has long been used to detect recent infection. the utility of antibodies directed against b19v ns1 protein has received less attention as a serological indicator of recent infection, although anti-b19v ns1 igg has been associated with persistent infection. | 2001 | 11418353 |
| diagnosis of human parvovirus b19 infections by detection of epitope-type-specific vp2 igg. | in the b19 vp2 molecule an immunodominant heptapeptide epitope has been detected, recently for which igg antibodies are synthesized exclusively in the acute phase of b19 infection. using this acute-phase-specific epitope (kyvtgin) a 2(nd)-generation epitope-type eia was developed, which compares serum igg activity for native vp2 capsids exhibiting conformational vp2 epitopes with igg activity for the kyvtgin epitope. in this study the diagnostic performance (clinical sensitivity and specificity) ... | 2001 | 11424127 |
| a comparison of oral fluid collection devices for use in the surveillance of virus diseases in children. | three oral fluid collection devices (orasure, omni-sal and oracol) were compared in terms of the quality of oral fluid collected by each device for antibody testing and their acceptability to participants. participants (143 children aged 3.5-5 y from north hertfordshire, uk, who had recently received dtap and mmr vaccination) were randomised to use one of the three types of collection device. oral fluid was collected by a parent who completed a short questionaire recording information on ease of ... | 2001 | 11429716 |
| congenital lymphocytic choriomeningitis virus infection: decade of rediscovery. | lymphocytic choriomeningitis virus (lcmv) is an underdiagnosed fetal teratogen. this diagnosis should be considered for infants and children with unexplained hydrocephalus, micro- or macrocephaly, intracranial calcifications, chorioretinitis, and nonimmune hydrops. the immunofluorescent antibody test is the only reasonable, commercially available, screening diagnostic tool. the differential diagnosis of congenital lcmv infection includes toxoplasmosis, rubella, cytomegalovirus, herpes simplex vi ... | 2001 | 11438904 |
| successful treatment of severe aplastic anemia associated with human parvovirus b19 and epstein-barr virus in a healthy subject with allo-bmt. | several reports have noted pancytopenia associated with human parvovirus b19 (pvb19) or ebstein-barr virus (ebv) infections in patients who have no history of immunodeficiency. to our knowledge, we report the first case of severe aplastic anemia associated with both ebv and pvb19 infections in a previously healthy 22-year-old man. he was admitted to our hematology service due to anemia and thrombocytopenia. he had no symptoms or signs of infections of these viruses. his bone marrow biopsy reveal ... | 2001 | 11443638 |
| screening of patients with complex regional pain syndrome for antecedent infections. | this study was designed to investigate whether complex regional pain syndrome type i (crps i) could be linked to any previous infection. | 2001 | 11444711 |
| lymphocyte recognition of human parvovirus b19 non-structural (ns1) protein: associations with occurrence of acute and chronic arthropathy? | immune recognition of recombinant parvovirus b19 non-structural (rns1) protein was studied by immunoblot and lymphoproliferative assays in blood from the following b19 seropositive groups: b19 infected (n = 14), b19 exposed but non-infected (n = 16), other illness with rash (n = 3), chronic arthropathy of unknown aetiology (n = 4) and healthy controls (n = 7). sera from 11 b19 seronegative subjects were also studied. sera collected at initial diagnosis or at the time of accidental b19 exposure i ... | 2001 | 11444773 |
| etiology and outcome of hydrops fetalis. | to identify the etiology and pregnancy outcome of hydrops fetalis in a cohort of pregnancies referred to a tertiary maternal fetal medicine center in the uk. these data allow the review of a large series of pregnancies affected by hydrops fetalis and emphasize the importance of investigation and then treatment of individual cases. this provides parents with improved information and especially specific prognostic information. | 2001 | 11444786 |
| [papular purpuric "gloves and socks" syndrome secondary to parvovirus b19 infection]. | | 2001 | 11447916 |
| association of parvovirus b19 with plasma cell-rich myocardial infiltrates after heart transplantation. | in this report we describe the development of plasma cell-rich myocardial infiltrates in association with a parvovirus b19 infection in a heart transplant patient. we hypothesize that the virus, either alone or in association with the cardiac allograft, may polarize the immune response in the direction of t helper 2 (th2) cells rather than the expected th1 cells. this favors the development of a humoral immune response and infiltration of the graft with plasma cells. | 2001 | 11448804 |
| papular-purpuric "gloves and socks" syndrome due to parvovirus b19: report of a case with unusual features. | we present a case of papular-purpuric "gloves and socks" syndrome (ppgss) in an adult male with acute parvovirus b19 infection. the patient displayed the classical features of fever, oral lesions, and purpura on hands and feet, but the purpuric lesions on the feet evolved to superficial skin necrosis, a feature not previously described in this syndrome. we believe this is the first reported case of ppgss occurring in brazil. | 2001 | 11452327 |
| acute parvovirus b19 infection during anti-retroviral therapy. | human parvovirus b19 (b19) has been described as a causative agent of chronic anemia in human immunodeficiency virus type-1 (hiv-1)-infected patients. we report an hiv-1 infected patient who had been receiving anti-retroviral therapy who showed sudden pancytopenia. primary b19 infection was confirmed by the detection of plasma viremia and seroconversion. although clearance required a prolonged period of time, the patient eventually cleared the b19 viral dna from the plasma. more than likely, hig ... | 2001 | 11455501 |
| human parvovirus b19 infection in hiv-positive patients. | parvovirus b19 infects predominantly erythroid cells, leading to transient inhibition of erythropoiesis. immunocompromised patients may be unable to produce neutralizing antibodies and may develop severe chronic anemia. epidemiological studies done on niterói population showed that b19 infection occurs periodically in late spring and summer. we report a study from 55 hiv infected patients attending an infectious diseases outpatient clinic in this city during a 5-month period in which b19 circula ... | 2001 | 11460208 |
| human parvovirus b19 induces cell cycle arrest at g(2) phase with accumulation of mitotic cyclins. | human parvovirus b19 infects specifically erythroid progenitor cells, which causes transient aplastic crises and hemolytic anemias. here, we demonstrate that erythroblastoid ut7/epo cells infected with b19 virus fall into growth arrest with 4n dna, indicating g(2)/m arrest. these b19 virus-infected cells displayed accumulation of cyclin a, cyclin b1, and phosphorylated cdc2 and were accompanied by an up-regulation in the kinase activity of the cdc2-cyclin b1 complex, similar to that in cells tre ... | 2001 | 11462027 |
| parvovirus b19 infection in a child with acute lymphoblastic leukemia during induction therapy. | immunocompromised children, including those undergoing chemotherapy treatment of malignant disease, are at particular risk for infection with parvovirus b19. however, these patients' attenuated immune responses may obscure the serologic and clinical manifestations of the infection. the authors describe a patient undergoing induction therapy for acute lymphoblastic leukemia whose parvovirus b19 infection was identified by the incidental detection of giant pronormoblasts and absence of normal matu ... | 2001 | 11464989 |
| induction of an invasive phenotype by human parvovirus b19 in normal human synovial fibroblasts. | to investigate the possible role of human parvovirus b19 as an etiologic agent in rheumatoid arthritis (ra), with particular emphasis on its ability to induce invasiveness in human synovial fibroblasts. | 2001 | 11465709 |
| guidance for control of parvovirus b19 infection in healthcare settings and the community. | interventions for parvovirus b19 infection need to balance the low risk of infection at a population level with the potential for serious adverse outcome for particular groups, notably the fetus, people with haemoglobinopathies and the immunocompromised. this guidance aims to assist the local decision-making process to be as evidence-based as the available evidence allows. | 1999 | 11469368 |
| parvovirus b19 infection in behçet's disease. | | 2001 | 11482311 |
| [five cases of erythema infectiosum in adults]. | the similarities between clinical features of erythema infectiosum and collagen disease or other viral infections prompted us to investigate clinical manifestations and laboratory data of parvovirus b19 (b19) infection in adults. we diagnosed all five patients as acute b19 infection by antibody assays. the age of patients ranged from 18 to 39 years old (mean 29), and all patients were female. all five patients showed high fever, arthralgia and edema of the extremities. four of the five patients ... | 2001 | 11494563 |
| [establishment of in situ hybridization technique for detection of parvovirus b19 and its clinical application]. | to establish in situ hybridization (ish) technique to detect parvovirus b19 and to know the viral location in cardiac tissue of congenital heart diseases (chd). | 2000 | 11498695 |
| nucleic acid testing: the international status of nat. | | 2001 | 11499968 |
| yield and future issues of nucleic acid testing. | despite the much lower actual yield than that estimated for hepatitis c virus (hcv) and human immunodeficiency virus (hiv) nucleic acid testing (nat)-only positives in the usa and germany, look-back procedures have revealed that no hcv transmission has occurred in germany since the introduction of nat. this indicates sufficient sensitivity of the pool-pcr approach. the slow ramp-up of hepatitis b virus (hbv) however, may require a different approach. it has been shown in germany that the pooling ... | 2001 | 11499978 |
| [the localization of parvovirus b19 in cardiac biopsy tissue of congenital heart disease]. | to explore the relationship between parvovirus b19 infection and congenital heart disease (chd), and distribution of parvovirus b 19 gene in cardiac tissue. | 2000 | 11503056 |
| the risk of infectious diseases among child care providers. | more than 1.1 million people were employed in out-of-home child care facilities in the united states in 1998, and this number is expected to increase over the next 10 years. this group consists primarily of women with a median age of 35. this review of the literature provides convincing evidence that these women have an excess risk of infection with a number of diseases, including cytomegalovirus, parvovirus b19, and, possibly, giardia. these workers also face an epidemic risk of such other infe ... | 2001 | 11506146 |
| [acute transitory intrafamilial erythroblastopenia and hereditary spherocytosis: role of parvovirus b19]. | acute parvovirus b19 infection induces a transient inhibition of erythroid cell formation, which may induce an erythroblastopenia crisis in patients suffering from chronic hemolytic anemia. | 2001 | 11508161 |
| detection of human parvovirus b19 in a patient with hepatitis. | parvovirus b19 has been associated by some investigators with cases of severe hepatitis. the aim of the present study was to determine the presence of active parvovirus b19 infection among 129 brazilian patients with non-a-e hepatitis. the patients were assayed for antibodies against parvovirus b19, igm class, by elisa. in igm-positive cases, parvovirus b19 dna was assayed by pcr in serum and liver tissue and parvovirus vp1 antigen in liver tissue was assayed by immunohistochemistry. antibodies ... | 2001 | 11514836 |
| novel pcr assay for differential detection and screening of erythrovirus b19 and erythrovirus v9. | diagnosis of erythrovirus b19 relies on serology and the detection of viral dna. these techniques were believed to detect all field isolates because erythrovirus b19 has been known to undergo little genetic variation (1-2%). recently, a distinct erythrovirus isolate termed v9, markedly different from erythrovirus b19 (>11% nucleotide disparity), was isolated from a child in france suffering from transient aplastic anemia. standard pcr assays and serological tests failed to demonstrate an acute e ... | 2001 | 11536245 |
| evidence for persistence of parvovirus b19 dna in livers of adults. | recent studies have suggested a pathogenic role of human parvovirus b19 (b19) in the development of acute fulminant liver failure in children. the hypothesis was based on the detection of b19 dna in 8 of 10 explanted livers of children requiring liver transplantation. in the present study, explanted livers from 43 adults selected at random undergoing orthotopic liver transplantation for various reasons were examined. pre-transplant sera were available from 40 patients of whom 35 (88%) were anti- ... | 2001 | 11536250 |
| persistence of parvovirus b19 dna in synovium of patients with haemophilic arthritis. | a progressive arthropathy develops commonly in haemophiliacs and its pathogenesis is not fully understood. human parvovirus b19 has been associated with several diseases including acute and chronic arthropathy and some studies suggest its implication in chronic inflammatory diseases of the joints such as rheumatoid arthritis. in haemophiliacs parvovirus b19 infection occurs very frequently because of its transmission with plasma derivatives. in order to assess a role of b19 virus in haemophilic ... | 2001 | 11536251 |
| parvovirus-associated arthritis. | | 2001 | 11545096 |
| association of human parvovirus b19 infection with acute meningoencephalitis. | to find out the incidence and clinical presentation of parvovirus b19 meningoencephalitis, we tested samples of cerebrospinal fluid from 162 patients (one from each patient) with undiagnosed meningoencephalitis, who presented between march, 1997, and march, 1998 (an outbreak period) using nested pcr for b19 genes. seven patients were positive; an incidence of 4.3%. five additional cases of meningoencephalitis were detected from other years. three patients with underlying disorders (haemophagocyt ... | 2001 | 11551584 |
| human parvovirus b19 in cord blood of premature infants. | we investigated whether intrauterine parvovirus b19 infection is associated with premature birth by evaluating parvovirus b19 antibodies and dna in umbilical cord blood from 76 premature infants. we performed enzyme-linked immunoadsorbent (elisa) and polymerase chain reaction (pcr) assays to detect b19-specific igm antibodies and parvovirus dna. no parvovirus dna was detected in cord blood sera, and no sample was positive for anti-parvovirus b19 igm antibodies. parvovirus appears unlikely to lea ... | 2001 | 11552176 |
| evidence on the infectious etiology of childhood leukemia: the role of low herd immunity (greece). | acute lymphoblastic leukemia (all) among children may be a rare outcome of a delayed non-specific infection in situations of overall low herd immunity. we evaluated the hypothesis as to whether newly diagnosed all cases, compared to their controls, are characterized by lower herd immunity, as reflected in a more seronegative spectrum to several agents, with the exception of a strongly positive response to a single infectious agent, assumed to trigger all. | 2001 | 11552712 |
| prediction of fetal anemia with doppler measurement of the middle cerebral artery peak systolic velocity in pregnancies complicated by maternal blood group alloimmunization or parvovirus b19 infection. | to confirm the relationship between the middle cerebral artery peak systolic velocity (mca psv) and hemoglobin values in fetuses at risk for anemia (due to maternal blood group alloimmunization or parvovirus b19 infection) and to investigate the clinical value of this method in the management of these pregnancies regardless of previous transfusions. | 2001 | 11555452 |
| fetal parvovirus b19 infection. | parvovirus b19 infection during pregnancy causes up to 27% cases of non-immune hydrops in anatomically normal fetuses. the virus is believed to cause arrest of maturation of red blood cell precursors at the late normoblast stage and also causes a decrease in the number of platelets. fetal anemia is presently thought to be responsible for the development of skin edema and effusions. myocarditis leading to heart failure may contribute to the development of fetal hydrops. we reviewed the literature ... | 2001 | 11555463 |
| parvovirus b19 infection in pediatric solid-organ and bone marrow transplantation. | the clinical significance of parvovirus b19 infection in pediatric solid-organ and bone marrow transplanted patients is unclear. the overall prevalence of parvovirus b19 infection in these patients is about 1-2% during the first year after transplantation. the most common symptom is anemia, but leukopenia and thrombocytopenia have also been observed. rare cases of hepatic dysfunction, myocarditis, vasculitis and respiratory failure have also been reported. whereas serology is of limited value ar ... | 2001 | 11560750 |
| cerebrovascular complications and parvovirus infection in homozygous sickle cell disease. | human parvovirus b19 infection causes most clinically defined aplastic crises in homozygous sickle cell (ss) disease. with transfusion support, the outcome is generally benign; however, cerebrovascular complications in close temporal association with b19-induced aplastic crises have been described. we carried out a retrospective review, between 1978 and 1999, of 346 aplastic crises in patients with ss disease attending the sickle cell clinic of the university hospital of the west indies, kingsto ... | 2001 | 11562626 |
| transient pancytopenia preceding acute lymphoblastic leukaemia (pre-all) precipitated by parvovirus b19. | a preleukaemic phase, typified by pancytopenia and bone marrow (bm) hypoplasia, is an uncommon but well-documented prelude to acute lymphoblastic leukaemia (pre-all) in children. parvovirus b19 (b19) exhibits a marked tropism to human bm and replicates only in erythroid progenitor cells acting as a confounding, but treatable agent in immunocompromised patients. we present the first case of b19-associated pre-all characterized by severe and recurring transient pancytopenia in a child who develope ... | 2001 | 11564067 |
| [severe infections in children with sickle cell disease: clinical aspects and prevention]. | sickle-cell disease (scd) is associated with frequent and often severe infections as a result of immune function impairment and functional asplenia. also, infection can trigger a vasoocclusive crisis. pneumonococcal bacteremia and meningitis due to s. pneumoniae are often lethal and justify the penicillin prophylaxis, which has provided a dramatic decrease in early mortality bacterial pneumonia is common in patients younger than four years, with most cases being due to s. pneumoniae, h. influenz ... | 2001 | 11582920 |
| status epilepticus due to human parvovirus b19 encephalitis in an immunocompetent adult. | human parvovirus (hpv) b19 is a rare cause of encephalitis in children and immunocompromised adults. the authors describe a case of an otherwise healthy woman who developed encephalitis complicated by prolonged status epilepticus. human parvovirus b19 infection was suggested by a prodromal, malar rash and established by the presence of specific igm and igg antibodies in the patient's serum. this is the first reported case of hpv b19 encephalitis in an immunocompetent adult. | 2001 | 11591864 |
| expression of p53 and ki-67 antigen in bone marrow giant proerythroblasts associated with human parvovirus b19 infection. | giant proerythroblasts are hallmarks of human parvovirus b19 infection. we attempted to characterize these cells in 5 patients with parvovirus b19-induced pure red cell aplasia using immunostaining of paraffin-embedded bone marrow sections with antibodies against erythroid-lineage-specific proteins, viral capsid antigen vp-1, and apoptosis- and cell-cycle-related proteins. giant proerythroblasts are immunohistochemically consistent with early erythroid precursors of cells in the differentiation ... | 2001 | 11594514 |
| human parvovirus b19 and fetal death. | | 2001 | 11597699 |
| human parvovirus b19 and fetal death. | | 2001 | 11597700 |
| renal involvement induced by human parvovirus b19 infection. | in an attempt to clarify the renal involvement induced by human parvovirus b19 (hpb19) infection, we investigated 6 adult patients with transient urinary abnormalities followed by erythema infectiosum. all patients had hpb19-specific igm antibody and showed mild proteinuria of 0.2-1.2 g/day with or without microscopic hematuria. in 5 patients a decrease of complement was present, and in 2 the circulating immune complex levels were elevated. all patients showed mild or moderate endocapillary prol ... | 2001 | 11598390 |
| [diagnosis of parvovirus b19 infection in hematological patients with partial red cell aplasia of the bone marrow]. | to assess diagnosis of parvovirus b19 infection (pi) in patients with aplastic crises by combined use of polymerase chain reaction (pcr) and enzyme immunoassay (eia) of specific igm and igg. | 2001 | 11599268 |
| antibodies to beta 2-glycoprotein i and cardiolipin with symptoms suggestive of systemic lupus erythematosus in parvovirus b19 infection. | parvovirus b 19 infection may mimic systemic lupus erythematosus (sle) in its clinical presentation. serological abnormalities during acute infection have been reported, including positive antinuclear antibodies and anti-doublestranded dna antibodies. we describe a patient who, in addition to the clinical and laboratory findings suggestive of sle, was found to have antibodies to cardiolipin and beta2 glycoprotein i. the symptoms resolved and the antibodies disappeared over a 9 month period. this ... | 2001 | 11669178 |
| [papular-purpuric gloves and socks syndrome associated with parvovirus b19 in an adult female]. | | 2001 | 11675926 |
| increase in b19 viral load prior to relapse of anaemia in an aids patient with persistent b19 infection. | anaemia caused by persistent parvovirus b19 infection can be remitted by intravenous immunoglobulin (ivig) treatment. however, maintenance ivig therapy is required in some aids patients as well as patients with combined immuno-deficiency because of relapse of anaemia. we used a simple semi-quantitative polymerase chain reaction (pcr) method to trace plasma b19 viral load after administration of ivig. we noted that the rebound of b19 viral load occurred 6 weeks prior to detection of anaemia. this ... | 2001 | 11676524 |
| persistent erythrovirus b19 urinary tract infection in an hiv-positive patient. | we report a urinary tract infection (uti) with erythrovirus b19 in an hiv-1-positive homosexual man persisting for more than 7 months after the decline of viremia after a primary infection. during the course of the uti, the patient complained of soreness in the kidney region and suffered from transient episodes of edema and hematuria. proteinuria and elevated serum concentrations of creatinine further substantiated the hypothesis of a renal focus of a persistent erythrovirus b19 infection. | 2001 | 11678936 |
| nucleic acid testing: update and applications. | nucleic acid testing (nat) holds the promise of closing the window of infectiousness for hepatitis c virus (hcv) and the human immunodeficiency virus (hiv) in the general blood supply. pioneering work by the source plasma industry with nat for hepatitis a virus (hav), hepatitis b virus (hbv), and parvovirus b19 suggests that, in the future, the risk of other viral infections may be reduced using similar technology. the european commission decree that, by july 1999, all source plasma for fraction ... | 2001 | 11685721 |
| human parvovirus b19 infection mimicking systemic lupus erythematosus in an adult patient. | we report a case of widespread immune activation with moderate cytopenia during acute infection with human parvovirus b19 in an adult female patient, in whom five criteria for the diagnosis of systemic lupus erythematosus were present at disease onset. our case is unusual due to the presence of a cutaneous rash mimicking leukocytoclastic vasculitis at presentation, moderate leukopenia with thrombocytopenia and the presence of a broad array of autoantibodies. diagnosis was established on the grou ... | 2001 | 11688361 |
| parvovirus arthropathy masquerading as the arthritis of behçet's disease. | | 2001 | 11688492 |
| a case of fetal parvovirus b19 myocarditis, terminal cardiac heart failure, and perinatal heart transplantation. | we report a case of fetal cardiomegaly secondary to myocarditis as a result of intrauterine parvovirus b19 infection. the fetus was delivered through caesarean section because of increasing deterioration of cardiac function at 33 + 3 weeks with reverse flow in the ductus venosus. four weeks later, a cardiac transplantation was carried out because of therapy-resistant dilative cardiomyopathy. this case shows that fetal parvovirus b19 infection may occur without anemia and myocarditis and does not ... | 2001 | 11694751 |
| maternal infections and subsequent psychosis among offspring. | we tested the hypothesis that maternal infections during pregnancy are associated with the subsequent development of schizophrenia and other psychoses in adulthood. | 2001 | 11695949 |
| parvovirus vectors for human gene therapy. | it has become increasingly clear that parvovirus-based vectors are a potentially safe and useful alternative to the more commonly used retroviral and adenoviral vectors. parvovirus vectors have been successfully used in phase i clinical trials for gene therapy of cystic fibrosis and hemophilia b, and several salient features of these vectors provide further support to the suggestion that their use in potential gene therapy of a wide variety of human diseases is imminent and perhaps well justifie ... | 2001 | 11699894 |
| new insights into the pathophysiology of acquired cytopenias. | this review addresses three related bone marrow failure diseases, the study of which has generated important insights in hematopoiesis, red cell biology, and immune-mediated blood cell injury. in section i, dr. young summarizes the current knowledge of acquired aplastic anemia. in most patients, an autoimmune mechanism has been inferred from positive responses to nontransplant therapies and laboratory data. cytotoxic t cell attack, with production of type i cytokines, leads to hematopoietic stem ... | 2000 | 11701533 |
| seropositivity of cytomegalovirus, parvovirus and rubella in pregnant women and recurrent aborters in leningrad county, russia. | the objectives of this paper were to assess the prevalence of different viral infections in relation to late abortions, stillbirths, and congenital malformations in sera from russian pregnant women and recurrent aborters in order to establish basic knowledge for future pregnancy care. | 2001 | 11703200 |
| lupus-like presentation of parvovirus b19 infection. | | 2001 | 11705435 |
| reactive haemophagocytic syndrome in children with inflammatory disorders. a retrospective study of 24 patients. | the reactive haemophagocytic syndrome (rhs) is a little-known life-threatening complication of rheumatic diseases in children. it reflects the extreme vulnerability of these patients, especially those with systemic-onset juvenile chronic arthritis (jca). this immunohaematological process may be triggered by events such as herpes virus infection and non-steroidal anti-inflammatory drug therapy. treatment has not been standardized. | 2001 | 11709613 |
| circulating tumour necrosis factor-alpha and interferon-gamma are detectable during acute and convalescent parvovirus b19 infection and are associated with prolonged and chronic fatigue. | to investigate whether cytokine responses may have a bearing on the symptoms and outcome of parvovirus b19 infection, circulating cytokines were measured during acute infection (n=51), follow-up of acute infection (n=39) and in normal healthy controls (n=50). at acute b19 virus infection (serum anti-b19 igm-positive), patients ranged in age from 4 to 54 years, with a mean age of 28.2 years. the male:female ratio was 1:4.1 and symptoms were rash (n=15), arthralgia (n=31), fatigue (n=8), lymphaden ... | 2001 | 11714978 |
| investigation of parvovirus b19 in cardiac tissue from patients with congenital heart disease. | to explore the correlation between human parvovirus b19 (hpv-b19) and congenital heart (chd) and to investigate the teratogenic effect of hpv-b19. | 1999 | 11721483 |
| new lightcycler pcr for rapid and sensitive quantification of parvovirus b19 dna guides therapeutic decision-making in relapsing infections. | detection of parvovirus b19 dna offers diagnostic advantages over serology, particularly in persistent infections of immunocompromised patients. a rapid, novel method of b19 dna detection and quantification is introduced. this method, a quantitative pcr assay, is based on real-time glass capillary thermocycling (lightcycler [lc]) and fluorescence resonance energy transfer (fret). the pcr assay allowed quantification over a dynamic range of over 7 logs and could quantify as little as 250 b19 geno ... | 2001 | 11724854 |
| fatal myocarditis associated with acute parvovirus b19 and human herpesvirus 6 coinfection. | we report on the case of a healthy young boy who developed a fulminant myocarditis due to acute coinfection with erythrovirus (parvovirus b19) and human herpesvirus 6 (hhv-6) in the absence of an antiviral immune response. we suggest that the hhv-6-induced immunosuppression enhanced dissemination of parvovirus b19, which led to fatal myocarditis. | 2001 | 11724892 |
| fetal and embryonic hemoglobins in erythroblasts from fetal blood and fetal cells enriched from maternal blood in fetal anemia. | to determine whether there is a delay or reversal in switch mechanisms from embryonic (e and z) to fetal (g) hemoglobins accompanying the erythroblastosis of anemic fetuses and whether an increased erythroblast count in fetal blood is associated with an increase in feto-maternal cell trafficking. | 2001 | 11726318 |
| asymmetric periflexural exanthem of childhood: who are you? | | 2001 | 11730033 |
| juvenile papular-purpuric gloves and socks syndrome. | papular-purpuric gloves and socks syndrome (ppgss) occurs mainly in young adults, but it should also be considered in the differential diagnosis of childhood exanthems. the case presented here is the youngest patient hitherto reported with ppgss and supports the concept of a viral genesis. | 2001 | 11730048 |
| asymmetric periflexural exanthem of childhood in an adult patient with parvovirus b19. | | 2001 | 11730065 |
| fibrosing cholestatic hepatitis possibly related to persistent parvovirus b19 infection in a renal transplant recipient. | | 2001 | 11733638 |
| haematological response to parvovirus b19 infection in homozygous sickle-cell disease. | infection with human parvovirus b19 is known to cause aplastic crises in patients with homozygous sickle-cell disease. we studied the haematological consequences of parvovirus b19 infection in 280 such patients who had been followed up from birth in jamaica. evidence of seroconversion was routinely sought with a baculovirus-based, enzyme immunoassay in serum samples taken during aplastic crises and in all stored annual serum samples. 70% of patients had seroconverted by age 20 years; of 177 infe ... | 2001 | 11734237 |
| prevention and management of infection in children with sickle cell anaemia. | sickle cell anaemia (sca) predisposes a child to infections for various reasons, including increased bone marrow turnover, poor perfusion and functional asplenia leading to decreased opsonisation of polysaccharide encapsulated organisms. bacteria and viruses that most frequently cause serious infections in children with sickle cell disease are streptococcus pneumoniae, haemophilus influenzae type b, salmonella spp., escherichia coli, staphylococcus aureus, mycoplasma pneumoniae, chlamydia pneumo ... | 2001 | 11735665 |
| detection of cytomegalovirus infection in a patient with febrile ulceronecrotic mucha-habermann's disease. | febrile ulceronecrotic mucha-habermann's disease (fumhd) is a severe and very rare variant of pityriasis lichenoides et varilioformis acuta, which is characterized by large coalescing, and ulceronecrotic maculopapules or plaques. morphological changes of the skin accompanied by persistent high fever and several constitutional symptoms have suggested virus infection in patients with fumhd. however, the available information of viral origin is limited. in this study we investigated the relationshi ... | 2001 | 11737435 |
| high level expression of recombinant mumps nucleoprotein in saccharomyces cerevisiae and its evaluation in mumps igm serology. | to develop improved reagents for mumps serology a high-level yeast expression system was employed to produce recombinant mumps nucleoprotein (rnp). the rnp was purified by cscl gradient centrifugation and yielded approximately 15 mg/l of yeast culture. electron microscopy of the rnp revealed characteristic herring-bone structures. the electrophoretic mobility of rnp in yeast cells was similar to native np in sds-page. monoclonal antibodies to rnp reacted with native mumps virus nucleoprotein by ... | 2002 | 11748668 |
| prophylaxis of hepatitis b reactivation using lamivudine in a patient receiving rituximab. | a 53-year-old man who had a history of fluminant hepatitis caused by precore mutant hepatitis b virus (hbv) was admitted to our hospital for the treatment of relapsed non-hodgkin's lymphoma in july 2000. at admission, serum levels of aspartate aminotransferase and alanine aminotransferase were normal, but he tested positive for hbs antigen. the titer was 64-fold by radioimmunoassay. we initiated lamivudine at a daily dose of 75 mg to prevent hbv proliferation during chemotherapy. by september 20 ... | 2001 | 11754421 |
| [a case of diffuse panbronchiolitis with sle complicated by fungal infection]. | we report a patient who developed sle during the course of diffuse panbronchiolitis (dpb) and had candidiasis later. the patient fulfilled the criteria for diagnosis of sle after appearance of fever and general peripheral arthritis. regarding serum virus antibody values at the time of sle diagnosis, igg and igm of human parvovirus b19 (b19) were positive by the eia test and also by the serum pcr test. for continuously pseudomonas aeruginozae in sputum cultures because of existing dpb, immunosupp ... | 2001 | 11766380 |
| simultaneous detection of measles virus, rubella virus, and parvovirus b19 by using multiplex pcr. | we describe here a multiplex reverse transcription-pcr (rtmnpcr) assay designed to detect and differentiate measles virus, rubella virus, and parvovirus b19. serial dilution experiments with vaccine strains that compared cell culture isolation of measles in b95 cells and rubella in rk13 cells showed sensitivity rates of 0.004 50% tissue culture infective dose (tcid(50)) for measles virus and 0.04 tcid(50) for rubella virus. this rtmnpcr can detect as few as 10 molecules for measles virus and rub ... | 2002 | 11773102 |
| diagnosing human parvovirus b19 infection: guidelines for test selection. | human parvovirus b19 is the cause of a common childhood disease that usually has a mild and self-limited course. complete viral replication and subsequent cell lysis are limited to early erythroid precursor cells expressing the globoside receptor. individuals with shortened red blood cell half-lives and immunocompromised or immunosuppressed patients, as well as pregnant women and developing fetuses, are at risk for severe anemia and/or persistent infection from human parvovirus b19. selection of ... | 2001 | 11774195 |
| high-titer screening pcr: a successful strategy for reducing the parvovirus b19 load in plasma pools for fractionation. | human parvovirus b19 (b19) is regarded as a potential risk factor for certain patient populations receiving plasma components. | 2001 | 11778063 |
| [the relationship between maternal infection with human parvovirus b19 and fetal death and congenital malformation]. | to study the relationship between matanal infection with human parvovirus b19 and fetal death and congenital malformation. | 2001 | 11778539 |
| baculovirus expression of erythrovirus v9 capsids and screening by elisa: serologic cross-reactivity with erythrovirus b19. | diagnosis of erythrovirus b19 (b19) relies on serology and the detection of viral dna. recently, a distinct erythrovirus isolate termed v9, markedly different from erythrovirus b19 (> 11% nucleotide disparity), was isolated. standard b19 pcr assays were inconclusive and serologic tests failed to categorize v9 as an acute b19-like infection. sequencing, combined with pcr studies, have since demonstrated the need for specific and differentiated techniques when examining samples for possible b19 or ... | 2002 | 11782935 |
| human parvovirus b19 and meningoencephalitis. | | 2001 | 11784660 |
| bone marrow samples from patients with aplastic anemia are not infected with parvovirus b19 and mycobacterium tuberculosis. | the majority of patients with aplastic anemia (aa) have an idiopathic form of the disease. the aim of this study was to detect the presence of parvovirus b19 dna and mycobacterium tuberculosis (mtb) dna by nested polymerase chain reaction (n-pcr) assays in the bone marrow biopsy samples from 30 patients with idiopathic aa. serologic assays for parvovirus b19 were based on indirect antibody capture enzyme-linked immunosorbent assay. our results indicate that neither parvovirus b19 dna nor mtb dna ... | 2002 | 11789728 |
| parvovirus b19 and parvovirus v9 are not associated with henoch-schönlein purpura in children. | based on single case reports, parvovirus b19 (b19) has repeatedly been proposed as an etiologic agent in patients with henoch-schönlein purpura (hsp), perhaps causing vasculitis by direct invasion of vascular endothelial cells because of the tissue distribution of the cellular b19 receptor. a cohort of children with hsp and other vasculitic diseases was investigated and compared with healthy control children to assess the role of b19 as well as parvovirus v9 (a putative emerging b19-like virus). | 2002 | 11791095 |
| the vp1 unique region of parvovirus b19 and its constituent phospholipase a2-like activity. | parvovirus b19 is the causative agent of erythema infectiosum. in addition, parvovirus b19 infection may be associated with other disease manifestations, namely, thrombocytopenia or granulocytopenia, spontaneous abortion or hydrops fetalis in pregnant women, acute and chronic arthritis, and systemic lupus erythematosus. based on sequence homology data, a phospholipase a2 motif has been identified in the vp1 unique region of parvovirus b19. (y. li et al., j. gen. virol. 82:2821-2825, 2001; z. zad ... | 2002 | 11799199 |
| [viral infections and pregnancy: contribution of amniotic fluid and blood samples]. | the main viral infections prenatally detected in fetuses are: cytomegalovirus, parvovirus b19, rubella virus and varicellazoster virus infections. prenatal diagnosis is based on the direct detection of the virus by culture (cmv), of its antigens or of its genome, essentially by pcr. this direct detection can be done either on fetal blood or on amniotic fluid. prenatal diagnosis can also be performed by detection of specific igm in fetal blood (rubella). non specific markers of viral infection ca ... | 2001 | 11802552 |
| a transgenic mouse model for non-immune hydrops fetalis induced by the ns1 gene of human parvovirus b19. | human parvovirus b19 (b19) infection during pregnancy is associated with the adverse foetal outcome known as non-immune hydrops fetalis (nihf). although b19 is known to infect erythroid-lineage cells in vivo as well as in vitro, the mechanism leading to the occurrence of nihf is not clear. to investigate the possible involvement of the b19 non-structural protein ns1 in nihf, three independent lines of transgenic mice were generated that expressed ns1 under the control of the cre-loxp system and ... | 2002 | 11807219 |
| [transient myelodysplasia associated with human parvovirus b19 infection in a child without underlying disease]. | human parvovirus b19 (hpv-b19) infection is known to frequently induce aplastic crisis in patients with hemolytic anemia, but rarely causes hematological disorders in healthy subjects. we report a 5-year-old boy who showed transient myelodysplasia associated with hpv-b19 infection. he was admitted with severe nasal bleeding, and laboratory data showed marked thrombocytopenia (1,000/microliter), anemia (hb: 8.4 g/dl), and mild leukopenia (3,000/microliter). a bone marrow smear revealed myelodyspl ... | 2001 | 11808077 |