| molecular and epigenetic analysis of the fragile histidine triad tumour suppressor gene in equine sarcoids. | sarcoids are peculiar equine benign tumours. their onset is associated with bovine papillomavirus type -1 or -2 (bpv-1/2) infection. little is known about the molecular interplay between viral infection and neoplastic transformation. the data regarding papillomavirus infections in human species show the inactivation of a number of tumour suppressor genes as basic mechanism of transformation. in this study the putative role of the tumour suppressor gene fragile histidine triad (fhit) in sarcoid t ... | 2012 | 22424615 |
| methods for the construction of human papillomavirus vectors. | a number of vector systems have been developed for the delivery of therapeutic genes into cells (1). many viral vectors suffer from the disadvantage of random integration into the chromosome, making the expression of the cloned genes dependent on the chromosomal context of the inserted dna. papillomaviruses (pvs) are potentially important vector systems because of their extrachromosomal replication in target cells. the pvs are small dna viruses that infect humans and a wide range of animals. hum ... | 1997 | 24493421 |
| bovine papillomavirus type 10 with a deletion associated with a lingual papilloma in a cow. | different types of papillomavirus usually cause papillomas in specific tissues. previously, bovine papillomavirus (bpv) type 10 has been associated specifically with cutaneous papillomas in cattle. in this study, bpv-10 was detected in a papilloma on the tongue of a cow. whole genome analysis demonstrated that the sequence of this bpv-10 strain had a 129 base pair deletion in the e1 open reading frame, which was confirmed by southern blot analysis, pcr and reverse transcriptase-pcr. | 2014 | 24361146 |
| complete genome sequence of deltapapillomavirus 4 (bovine papillomavirus 2) from a bovine papillomavirus lesion in amazon region, brazil. | the complete genome sequence of bovine papillomavirus 2 (bpv2) from brazilian amazon region was determined using multiple-primed rolling circle amplification followed by illumina sequencing. the genome is 7,947 bp long, with 45.9% gc content. it encodes seven early (e1, e2,e4, e5, e6,e7, and e8) and two late (l1 and l2) genes. the complete genome of a bpv2 can help in future studies since this bpv type is highly reported worldwide although the lack of complete genome sequences available. | 2016 | 27074259 |
| [sequence and structural analyses of the complete genome of bovine papillomavirus 2 genotype aks-01 strain from skin samples of cows in southern xinjiang, china]. | to study the complete genomic sequence, genomic characteristics, and genetic variation of the bovine papillomavirus 2 genotype (bpv-2) aks-01 strain at the molecular level, genotyping of this strain from the skin samples of cows in southern xinjiang (china) was first detected by the polymerase chain reaction with fap59/fap64 primers. based on the complete genome of the bpv-2 reference strain, specific primers and sequencing primers were designed, and the complete genome of the aks-01 strain ampl ... | 2015 | 26524909 |
| detection and quantification of bovine papillomavirus type 2 in urinary bladders and lymph nodes in cases of bovine enzootic hematuria from the endemic region of azores. | bovine enzootic hematuria (beh) is a disease with a severe impact on production indexes and characterized by the development of bovine urinary bladder tumors, particularly in the azores archipelago. the purpose of this study was to investigate and quantify bpv2 tissue distribution in bovine urinary bladder tumors, normal bladders, and iliac lymph nodes of cattle from the azores. a real-time pcr system targeting the l1 gene was developed and allowed for the specific detection of the virus. bpv2 d ... | 2015 | 26003566 |
| first report of a variant bovine papillomavirus type 2 (bpv-2) in cattle in the iberian peninsula. | infections caused by bovine papillomavirus (bpv) have been described worldwide. some types, like bpv-1 and bpv-2, have been reported in association with skin warts and fibropapillomas in cattle and sarcoids in equids. in this study we have investigated the presence of bpv in cutaneous warts isolated from a steer in spain. cutaneous fibropapillomatosis was confirmed by histopathological analysis. complete genome was amplified by multiple-primed rolling circle and the l1, e5 and e6 genes were sequ ... | 2014 | 25273965 |
| bovine papillomavirus type 2 (bpv-2) e5 oncoprotein binds to the subunit d of the v₁-atpase proton pump in naturally occurring urothelial tumors of the urinary bladder of cattle. | active infection by bovine papillomavirus type 2 (bpv-2) was documented for fifteen urinary bladder tumors in cattle. two were diagnosed as papillary urothelial neoplasm of low malignant potential (punlmp), nine as papillary and four as invasive urothelial cancers. | 2014 | 24586417 |
| bovine papillomavirus type 2 infection and a series of mesenchymal tumors of the urinary bladder in cattle. | this report describes the histopathology of two hundred and fifty-three mesenchymal tumors of the urinary bladder in cattle grazing on lands rich in bracken fern. approximately 80% were hemangiomas and angiosarcomas. hemangioma (capillary, cavernous, and large vessels) was the most frequent mesenchymal tumor and was more common than angiosarcoma. although the appearance of endothelial cells can vary remarkably, epithelioid angiosarcomas, often containing multinucleated cells, were the most frequ ... | 2013 | 23862156 |
| bovine papillomavirus type 2 infection and microscopic patterns of urothelial tumors of the urinary bladder in water buffaloes. | microscopic patterns of thirty-four urothelial tumors of the urinary bladder of water buffaloes from the marmara and black sea regions of turkey are here described. all the animals grazed on lands rich in bracken fern. histological diagnosis was assessed using morphological parameters recently suggested for the urinary bladder tumors of cattle. papillary carcinoma was the most common neoplastic lesion (22/34) observed in this study, and low-grade carcinoma was more common (seventeen cases) than ... | 2013 | 23762866 |
| productive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes. | papillomaviruses (pvs) are highly epitheliotropic as they usually establish productive infections within squamous epithelia of the skin, the anogenital tract and the oral cavity. in this study, early (e) and late (l) protein expression of bovine papillomavirus type 2 (bpv-2) in the urothelium of the urinary bladder is described in cows and water buffaloes suffering from naturally occurring papillomavirus-associated urothelial bladder tumors. | 2013 | 23667460 |
| bovine papillomavirus type 2 infects the urinary bladder of water buffalo (bubalus bubalis) and plays a crucial role in bubaline urothelial carcinogenesis. | bovine papillomavirus type 2 (bpv-2) has been shown to infect and play a role in urinary bladder carcinogenesis of buffaloes grazed on pastures with ferns from the marmara and black sea regions of turkey. bpv-2 dna has been found in both neoplastic and non-neoplastic lesions of the urinary bladder. furthermore, this virus may be a normal inhabitant of the urinary bladder since bpv-2 dna has also been detected in clinically normal buffaloes. the viral activation by fern immunosuppressant or carci ... | 2013 | 23100367 |
| productive infection of bovine papillomavirus type 2 in the placenta of pregnant cows affected with urinary bladder tumors. | papillomaviruses (pvs) are believed to be highly epitheliotropic as they usually establish productive infections within stratified epithelia. in vitro, various pvs appear to complete their entire life-cycle in different trophoblastic cell lines. in this study, infection by and protein expression of bovine papillomavirus type 2 (bpv-2) in the uterine and chorionic epithelium of the placenta has been described in four cows suffering from naturally occurring papillomavirus-associated urothelial bla ... | 2012 | 22479413 |
| bovine papillomavirus type 4 l1 gene transfection in a drosophila s2 cell expression system: absence of l1 protein expression. | the development of a bovine papillomavirus (bpv) vaccine is an outstanding challenge. bpv protein l1 gene transfection in the drosophila melanogaster s2 cell expression system failed to produce l1 protein notwithstanding correct l1 gene insertion. severe genetic inbalance in the host cell line, including cytogenetic alterations, may account for the lack of protein expression. | 2008 | 24031166 |
| phosphorylation of the bovine papillomavirus e2 protein on tyrosine regulates its transcription and replication functions. | papillomaviruses are small, double-stranded dna viruses that encode the e2 protein, which controls transcription, replication, and genome maintenance in infected cells. posttranslational modifications (ptms) affecting e2 function and stability have been demonstrated for multiple types of papillomaviruses. here we describe the first phosphorylation event involving a conserved tyrosine (y) in the bovine papillomavirus 1 (bpv-1) e2 protein at amino acid 102. while its phosphodeficient phenylalanine ... | 2017 | 27807239 |
| requirement for the e1 helicase c-terminal domain in papillomavirus dna replication in vivo. | the papillomavirus (pv) e1 helicase contains a conserved c-terminal domain (ctd), located next to its atp-binding site, whose function in vivo is still poorly understood. the ctd is comprised of an alpha helix followed by an acidic region (ar) and a c-terminal extension termed the c-tail. recent biochemical studies on bovine papillomavirus 1 (bpv1) e1 showed that the ar and c-tail regulate the oligomerization of the protein into a double hexamer at the origin. in this study, we assessed the impo ... | 2016 | 26739052 |
| interactions between e6, fak, and git1 at paxillin ld4 are necessary for transformation by bovine papillomavirus 1 e6. | bovine papillomavirus 1 e6 interacts with two similar proteins that regulate cell attachment and cell migration called paxillin (pxn) and hic-5 (also known as hic5, ara55, hic-5, tsc-5, and tgfb1i1). despite the similarity between hic-5 and paxillin, paxillin is required for e6 to transform mouse embryo fibroblasts while hic-5 is not. using mutants of paxillin, we found that dynamic competitive interactions between e6, focal adhesion kinase, and the git1 arf-gap protein for binding to paxillin a ... | 2014 | 24942580 |
| e1-mediated recruitment of a uaf1-usp deubiquitinase complex facilitates human papillomavirus dna replication. | the human papillomavirus (hpv) e1 helicase promotes viral dna replication through its dna unwinding activity and association with host factors. the e1 proteins from anogenital hpv types interact with the cellular wd repeat-containing factor uaf1 (formerly known as p80). specific amino acid substitutions in e1 that impair this interaction inhibit maintenance of the viral episome in immortalized keratinocytes and reduce viral dna replication by up to 70% in transient assays. in this study, we dete ... | 2014 | 24850727 |
| sarcoid-derived fibroblasts: links between genomic instability, energy metabolism and senescence. | bovine papillomavirus 1 (bpv-1) is a well recognized etiopathogenetic factor in a cancer-like state in horses, namely equine sarcoid disease. nevertheless, little is known about bpv-1-mediated cell transforming effects. it was shown that bpv-1 triggers genomic instability through dna hypomethylation and oxidative stress. in the present study, we further characterized bpv-1-positive fibroblasts derived from sarcoid tumors. the focus was on cancer-like features of sarcoid-derived fibroblasts, incl ... | 2014 | 24148276 |
| spontaneous cutaneous papillomatosis in yaks and detection and quantification of bovine papillomavirus-1 and -2. | seven clinical cases of cutaneous papillomatosis in yaks were studied in arunachal pradesh, india. sporadic, single or a chain of multiple varying size warts appeared around the eyes or on the body. predominant site of warts was around eyes. histopathologically, these cases were diagnosed as fibropapilloma. it was confirmed by the detection of bpv-1 and bpv-2 or their mixed infection by pcr and sequencing. quantitative sybr green real-time pcr detected comparatively lower viral dna copy number i ... | 2013 | 23206264 |
| oral administration of hpv-16 l2 displayed on lactobacillus casei induces systematic and mucosal cross-neutralizing effects in balb/c mice. | the human papillomavirus (hpv) minor capsid protein, l2, is a good candidate for prophylactic vaccine development because l2-specific antibodies have cross-neutralizing activity against diverse hpv types. here, we developed a hpv mucosal vaccine candidate using the poly-γ-glutamic acid synthetase a (pgsa) protein to display a partial hpv-16 l2 protein (n-terminal 1-224 amino acid) on the surface of lactobacillus casei (l. casei). the oral immunization with l. casei-l2 induced productions of l2-s ... | 2012 | 22426329 |
| genome characterization of a bovine papillomavirus type 5 from cattle in the amazon region, brazil. | papillomaviruses are small and complex viruses with circular dna genome that belongs to the papillomavirus family, which comprises at least 39 genera. the bovine papillomavirus (bpv) causes an infectious disease that is characterized by chronic and proliferative benign tumors that affect cattle worldwide. in the present work, the full genome sequence of bpv type 5, an epsilonpapillomavirus, is reported. the genome was recovered from papillomatous lesions excised from cattle raised in the amazon ... | 2017 | 27817150 |
| enhancing antitumor immunogenicity of hpv16-e7 dna vaccine by fusing dna encoding e7-antigenic peptide to dna encoding capsid protein l1 of bovine papillomavirus. | human papillomavirus (hpv) has been identified as the primary etiologic factor of cervical cancer, the fourth leading cause of cancer death in females worldwide. we have previously shown that coadministration of dna encoding l1 capsid protein of bovine papillomavirus (bpv) can enhance the antigen-specific immune response elicited by a therapeutic hpv16-e7 dna vaccination. in this study, we sought to generate and evaluate the immunogenicity of a therapeutic hpv16-e7 dna vaccine that encodes the f ... | 2017 | 28852471 |
| two transmembrane dimers of the bovine papillomavirus e5 oncoprotein clamp the pdgf β receptor in an active dimeric conformation. | the dimeric 44-residue e5 protein of bovine papillomavirus is the smallest known naturally occurring oncoprotein. this transmembrane protein binds to the transmembrane domain (tmd) of the platelet-derived growth factor β receptor (pdgfβr), causing dimerization and activation of the receptor. here, we use rosetta membrane modeling and all-atom molecular dynamics simulations in a membrane environment to develop a chemically detailed model of the e5 protein/pdgfβr complex. in this model, an active ... | 2017 | 28808001 |
| phylogenetic analysis of a partial l1 gene from bovine papillomavirus type 1 isolated from naturally occurring papilloma cases in the northwestern region of turkey. | this study was aimed at the molecular characterisation of bovine papillomavirus type 1 (bpv-1) isolated from papilloma cases in the northwestern region of turkey. bpv-1 is a widely occurring oncogenic virus in cattle and is associated with benign epithelial neoplasia which causes significant economic losses in dairy and beef cattle because of treatment costs. in this study, 29 suspected papilloma specimens were collected from cattle in northwestern turkey. these samples underwent molecular chara ... | 2017 | 28697610 |
| type-specific l1 virus-like particle-mediated protection of horses from experimental bovine papillomavirus 1-induced pseudo-sarcoid formation is long-lasting. | equine sarcoids are common therapy-resistant skin tumours induced by bovine papillomavirus type 1 or 2 (bpv1, bpv2) infection. we have previously shown that prophylactic vaccination with bpv1 l1 virus-like particles (vlps) efficiently protects horses from experimental bpv1-induced pseudo-sarcoid development. here, we assessed bpv1 l1 vlp vaccine-mediated long-term protection from experimental tumour formation in seven horses 5 years after immunization with three different doses of bpv1 l1 vlps, ... | 2017 | 28635592 |
| a novel bovine papillomavirus type in the genus dyokappapapillomavirus. | papillomaviruses are a diverse group of viruses that are known to infect a wide range of animal species. bovine papillomaviruses (bpvs) are divided into at least 21 genotypes (bpv1 to bpv21), with most bpv isolates/strains described to date belonging to one of four genera, including deltapapillomavirus, xipapillomavirus, epsilonpapillomavirus and dyoxipapillomavirus. here, we describe the identification and genetic characterization of a new bpv type in the genus dyokappapapillomavirus. a farm i ... | 2017 | 28616671 |