| the number of morphological synapses between neurons does not predict the strength of their physiological synaptic interactions: a study of dendrites in the nematode ascaris suum. | previous electrophysiological and anatomical studies of ascaris suum motor neurons demonstrated a strong correlation between functional interactions and the presence of anatomically defined synapses. however, one example of a physiologically robust synaptic connection was encountered for which no anatomical evidence of direct chemical synapses was found. this involved synaptic transmission from an identified excitatory motor neuron to its inhibitory partner. in this study, pressure injection of ... | 2001 | 11268011 |
| localised myelitis caused by visceral larva migrans due to ascaris suum masquerading as an isolated spinal cord tumour. | | 2001 | 11271166 |
| characterization of 5-ht receptors in the parasitic nematode, ascaris suum. | the pharmacological profiles of the 5-hydroxytryptamine (5-ht) receptors on ascaris suum pharyngeal and somatic body wall muscles were investigated. the mechanisms involved following activation of these receptors were also studied. 5-ht activated and maintained pumping in isolated pharynxes with an ec-50 value of 44+/-1.7 microm. the 5-ht agonists, tryptamine, sumatriptan 8-oh-dpat and 5-carboxyamidotryptamine all failed to stimulate pumping. the 5-ht2 antagonist, ketanserin, initially excited a ... | 2001 | 11272652 |
| characterization of a putative nitric oxide synthase in the neuromuscular system of the parasitic nematode, ascaris suum. | in this paper we report on the biochemical presence of nitric oxide synthase (nos)-like activity in ascaris suum tissue and examine the pharmacological effect of no donors on a. suum muscle strip preparation. nos activity was determined by monitoring the formation of [3h]citrulline from [3h]l-arginine and no formation via the oxyhaemoglobin assay. neuromuscular tissue from a. suum which stained positively for nadph diaphorase, contained nos activity. neither nos activity nor nadph diaphorase sta ... | 2001 | 11272653 |
| possible involvement of g(i3) protein in augmented contraction of bronchial smooth muscle from antigen-induced airway hyperresponsive rats. | to investigate a possible involvement of pertussis toxin (ptx)-sensitive heterotrimeric g proteins in the pathogenesis of airway hyperresponsiveness, the effect of ptx treatment on the augmented contractile response to acetylcholine (ach) in bronchial smooth muscle of antigen-induced airway hyperresponsive rats was determined. in bronchial smooth muscle of airway hyperresponsive rats that were actively sensitized and repeatedly challenged with 2,4-dinitrophenylated ascaris suum antigen, ach-indu ... | 2001 | 11274979 |
| the dangers of using single locus markers in parasite epidemiology: ascaris as a case study. | molecular markers are used widely to discriminate between closely related species of parasites, and in many cases a single locus is used for this purpose. this article aims to show how molecular data derived from a single genetic marker or linkage group - in this case mitochondrial dna - can lead to ambiguous conclusions and to illustrate how a multilocus approach has enhanced our understanding of the epidemiology of two closely related parasites, the nematodes ascaris suum, which infects pigs, ... | 2001 | 11282508 |
| immunohistochemical localization and differentiation of phosphocholine-containing antigens of the porcine, parasitic nematode, ascaris suum. | the glycolipids of ascaris suum represent either neutral, zwitterionic or acidic structures. the acidic fraction comprises a sulphatide and an unusual phosphoinositolglycosphingolipid (lochnit et al. 1998b). the sulphatide was previously localized to the hypodermis, contractile zone of somatic muscle cells and the external musculature of the female uterus, whereas the presence of the phosphoinositolglycosphingolipid species was restricted to the intestine. the neutral and zwitterionic components ... | 2001 | 11289072 |
| in vitro fermentation of cellulosis amorphous and meadow hay in experimentally ascaris suum-infected lambs. | the objective of this experiment was to determine the effect of rumen inocula from experimentally ascaris suum (as)-infected lambs on cellulose amorphous (ca) and meadow hay (mh) used as substrates during 72h incubation in vitro. the rumen inocula were obtained post-mortem from eight lambs that had been experimentally infected with 1000 as eggs per lamb daily for 3 weeks. samples of rumen inocula were obtained from the lambs weekly throughout the experiment for 8 weeks. two lambs were kept as un ... | 2001 | 11295398 |
| regional immune responses with stage-specific antigen recognition profiles develop in lymph nodes of pigs following ascaris suum larval migration. | the early life-cycle of the pig round worm, ascaris suum, involves well-defined larval development in the liver, lungs and finally the small intestine. distinct regional immune responses to larval antigens of a. suum were observed in the draining lymph nodes of immunized and challenged pigs during larval migration. this was reflected in a transient enlargement of the stimulated lymph nodes, due to increases in numbers of b cells and cd4 t cells, and the production of a. suum-specific antibody by ... | 2001 | 11298295 |
| pharmacotherapy of ascariasis. | ascaris lumbricoides, the most frequent human intestinal nematode, is the causative agent of ascariasis, with an estimated worldwide prevalence of over one billion people, especially in moist tropical and subtropical regions, but also in cooler climates. although characterised with low morbidity and mortality rates, the global prevalence of ascariasis still results in approximately 20,000 deaths annually, primarily as a consequence of intestinal obstruction. in humans, transmission usually occur ... | 2001 | 11336582 |
| ascaris suum-derived products induce human neutrophil activation via a g protein-coupled receptor that interacts with the interleukin-8 receptor pathway. | infection with tissue-migrating helminths is frequently associated with intense granulocyte infiltrations. several host-derived factors are known to mediate granulocyte recruitment to the tissues, but less attention has been paid to how parasite-derived products trigger this process. parasite-derived chemotactic factors which selectively recruit granulocytes have been described, but nothing is known about which cellular receptors respond to these agents. the effect of products from the nematodes ... | 2001 | 11349070 |
| comparative metabolism of albendazole and albendazole sulphoxide by different helminth parasites. | albendazole (abz) is a broad-spectrum benzimidazole anthelmintic widely used in human and veterinary medicine. the aim of the current work was to characterise the sulphoxidative metabolism of abz, and the sulphoreduction of abz sulphoxide (abzso), by microsomal (ms) and cytosolic (cyt) fractions of three different helminth species: fasciola hepatica, moniezia expansa and ascaris suum. after the incubation assays, parasite material was analysed by hplc to characterise the metabolic product formed ... | 2001 | 11355675 |
| nitric oxide synthase in filariae: demonstration of nitric oxide production by embryos in brugia malayi and acanthocheilonema viteae. | the radical gas nitric oxide (no) is synthesized by nitric oxide synthase (nos) from l-arginine and molecular oxygen. nitric oxide is an important signaling molecule in invertebrate and vertebrate systems. previously we have shown that nos is localized to more tissues in brugia malayi than has been reported in ascaris suum. in this paper, we analyze the distribution of nos in acanthocheilonema viteae, a filarial nematode that differs from b. malayi in that a. viteae females release microfilariae ... | 2001 | 11384164 |
| determination of metabolite and regulatory enzyme levels in dirofilaria immitis and ascaris suum: a comparative study. | studies on metabolite levels in dirofilaria immitis revealed similarities in several metabolites with those of ascaris suum. the glycogen level in the filariid was however 3-4 times lower than that in a. suum. levels of three regulatory enzymes were also determined in d. immitis and compared with those in a. suum. the activities of hexokinase and phosphofructokinase were similar. however, the levels of glycogen phosphorylase b appeared to be much lower in the filariid than in a. suum. the subtle ... | 2000 | 11391834 |
| effect of iron deficiency on trichuris suis and ascaris suum infections in pigs. | the objective of this experiment was to detect a possible interaction between iron deficiency and intestinal nematode infections. we report on a 2 x 2 study where thirty-one 10-week-old pigs fed a low or a normal iron diet were infected with both trichuris suis (4500 eggs) and ascaris suum (1200 eggs). no significant difference was detected between diet groups with respect to parasitological parameters for a. suum or the total number of adult t. suis recovered at necropsy 10 weeks p.i. however, ... | 2001 | 11393833 |
| very high resolution structure of a trematode hemoglobin displaying a tyrb10-tyre7 heme distal residue pair and high oxygen affinity. | monomeric hemoglobin from the trematode paramphistomum epiclitum displays very high oxygen affinity (p(50)<0.001 mm hg) and an unusual heme distal site containing tyrosyl residues at the b10 and e7 positions. the crystal structure of aquo-met p. epiclitum hemoglobin, solved at 1.17 a resolution via multiwavelength anomalous dispersion techniques (r-factor=0.121), shows that the heme distal site pocket residue tyrb10 is engaged in hydrogen bonding to the iron-bound ligand. by contrast, residue ty ... | 2001 | 11399085 |
| autofluorescence microscopy for the detection of nematode eggs and protozoa, in particular isospora suis, in swine faeces. | parasites from swine faeces were examined for autofluorescence. oocysts of eimeria polita, e. scabra and isospora suis, cysts of balantidium coli and eggs of oesophagostomum dentatum, strongyloides ransomi and trichuris suis (but not those of ascaris suum) emitted light after excitation with uv light. i. suis oocyst counts in mcmaster chambers utilising autofluorescence were compared to those from conventional bright field microscopy. similarly, faecal smears containing i. suis were examined usi ... | 2001 | 11403385 |
| resistance against migrating ascaris suum larvae in pigs immunized with infective eggs or adult worm antigens. | resistance to ascaris suum infections was investigated in 8- and 15-week-old iberian pigs. groups of 3 or 5 pigs were immunized weekly for 6 weeks with antigens of adult a. suum: a 97 kda body wall (bw) fraction, a 42 kda fraction of pseudocoelomic fluid (pf) or a 14 kda pf-fraction; or were inoculated with increasing doses of infective eggs (500-20,000), with or without abbreviation by pyrantel pamoate. all immunized pigs and unimmunized control pigs, were challenged with 10,000 infective eggs ... | 2001 | 11444623 |
| surface properties of ascaris suum eggs: hydrophobic potential and lewis acid-base interactions. | the cell surface properties of ascaris suum eggs were investigated to determine the hydrophobic properties of eggs of different origins. hydrophobic potential measurements were based on adhesion to hydrocarbons. freshly laid eggs with or without the external coat and eggs that had been stored for 3 months at -20 degrees c were tested. the hydrophobic potential differed according to the hydrocarbon used (hexadecane or octane) and the eggs tested (stored or fresh). the hydrophobic potential with o ... | 2001 | 11451656 |
| identification of larval-stage antigens of ascaris suum recognized with immune sera from pigs. | antigens were identified from the third-stage larvae (l3) and lung-stage larvae of ascaris suum by two-dimensional immunoblot method with antisera obtained from pigs that received chemically abbreviated ascaris suum larval infections. forty-seven and 13 spots were recognized as antigens from the l3 and lung-stage larvae, respectively. their apparent molecular weight ranged from 20 to 101 kda and their isoelectric point from 3.6 to 8.0. the present study provides a framework for further molecular ... | 2001 | 11459018 |
| involvement of neuropeptides in the allergic nasal obstruction in guinea pigs. | the purposes of the present study were i) to determine whether neuropeptides induce the nasal obstruction in guinea pigs, and ii) to examine the possible involvement of neuropeptides in allergic nasal obstruction. the decrease in nasal cavity volume was determined by acoustic rhinometry as an index of nasal obstruction. in non-sensitized guinea pigs, substance p (sp), neurokinin a (nka) and calcitonin gene-related peptide (cgrp) caused the nasal obstruction 10 to 30 min after their intranasal ap ... | 2001 | 11459122 |
| the influence of stocking rate on transmission of helminth parasites in pigs on permanent pasture during two consecutive summers. | this study was made to elucidate the transmission of nematode infections in outdoor pigs at different stocking rates during two consecutive seasons. five pigs (group 1a) inoculated with low doses of oesophagostomum dentatum, ascaris suum, and trichuris suis and five helminth-naïve pigs (group 1b) were turned out together in june 1996 on each of four pastures at stocking rates of 100, 240 (two pastures) and 576m(2) per pig, respectively. the pigs were slaughtered in early october, and pasture inf ... | 2001 | 11470180 |
| production and characterization of a monoclonal antibody against an ascaris suum allergenic component. | ascaris suum allergenic components (piii) separated by gel filtration chromatography of an adult worm extract were used to immunize balb/c mice. popliteal lymph node cells taken from the immunized animals were fused with sp2/o myeloma cells using polyethylene glycol (mw 1450) as fusogen. the hybridomas were cultured in hat-containing medium and cloned at limiting dilutions. supernatants from the growing hybrids were screened by elisa using plates coated with piii or the a. suum crude extract. th ... | 2001 | 11471042 |
| possible presence of common tyvelose-containing glycans in trichinella l1 larvae and embryonated eggs of several nematodes. | a monoclonal antibody (mab us4) recognising an epitope containing tyvelose within the t. spiralis l-1 muscle larvae (tsl-1) antigens was tested in western-blot against various antigenic preparations from different stages of the following nematodes: t. spiralis (l1, adult), t. muris (egg, l1, l3, adult), ascaris suum (egg, adult), toxocara canis (egg, adult), anisakis simplex (l3) and haemochus contortus (egg). positive reaction was present in antigen preparations from l1 larvae of t. spiralis an ... | 2001 | 11484333 |
| preclinical efficacy and safety of mepolizumab (sb-240563), a humanized monoclonal antibody to il-5, in cynomolgus monkeys. | allergic respiratory diseases are characterized by large numbers of eosinophils and their reactive products in airways and blood; these are believed to be involved in progressive airway damage and remodeling. il-5 is the principal cytokine for eosinophil maturation, differentiation, and survival. mepolizumab (sb-240563), a humanized monoclonal antibody (mab) specific for human il-5, is currently in clinical trials for treatment of asthma. | 2001 | 11496242 |
| mutagenic and chemical modification of the aba-1 allergen of the nematode ascaris: consequences for structure and lipid binding properties. | the polyprotein allergens/antigens of nematodes (npas) are the only lipid binding proteins known to be produced as polyproteins. cleavage of the large polyprotein precursors at regularly spaced proteinase cleavage sites produces 10 or 11 individual protein units of approximately 15 kda. the sequences of these units are highly diverse within and between species, but there are five absolutely or strongly conserved amino acid positions (trp15, gln20, leu42, cys64, and cys120). we have tested the ro ... | 2001 | 11502186 |
| optimization of the agar-gel method for isolation of migrating ascaris suum larvae from the liver and lungs of pigs. | experiments on use of an agar-gel method for recovery of migrating ascaris suum larvae from the liver and lungs of pigs were conducted to obtain fast standardized methods. subsamples of blended tissues of pig liver and lungs were mixed with agar to a final concentration of 1% agar and the larvae allowed to migrate out of the agar-gel into 0.9% nacl at 38 degrees c. the results showed that within 3 h more than 88% of the recoverable larvae migrated out of the liver agar-gel and more than 83% of t ... | 2001 | 11503373 |
| isolation and preliminary biological assessment of aadgaplirfamide and svpgvlrfamide from caenorhabditis elegans. | to date, 9 fmrfamide-related peptides (farps) have been structurally characterised from caenorhabditis elegans. radioimmunometrical screening of an ethanolic extract of c. elegans revealed the presence of two additional farps that were purified by reverse-phase hplc and subjected to edman degradation analysis and gas-phase sequencing. unequivocal primary structures for the two farps were determined as ala-ala-asp-gly-ala-pro-leu-ile-arg-phe-nh(2) and ser-val-pro-gly-val-leu-arg-phe-nh(2). using ... | 2001 | 11527423 |
| [relationship between the prevalence of hepatic milk spots in pig and the egg density of ascaris suum in kitakyushu municipal meat inspection and control center]. | the hepatic milk spots of pigs are chronic interstitial hepatitis, and are caused by the migration of pig ascarid worm, ascaris suum, into the liver. a large number of livers are condemned in meat inspection centers as defective food once they are found to have developed milk spots, and thus the economic loss is great. eggs of ascaris suum from 120 caecum feces of slaughtered pigs were detected by the nylon mesh filtration (makiya) technique and they were related to the condemnation data of the ... | 2001 | 11570049 |
| release of hyaluronidase during in vitro development of ascaris suum from the third to fourth larval stage. | an enzyme that degraded glycosaminoglycan hyaluronic acid was released during in vitro development of ascaris suum l3 to l4. the enzyme did not hydrolyze glycosaminoglycan chondroitin sulfate a. one molecular form of hyaluronidase was detected, with a molecular weight estimated at 47.8 +/- 8.6 kda by sucrose density gradient centrifugation and at 55.0 +/- 1.3 kda by substrate sds-page zymography. activity of the enzyme was optimal between ph 5.0 and 6.0, and was present at neutral ph. hyaluronid ... | 2001 | 11570551 |
| purification of anisakis simplex antigen by affinity chromatography. | in order to improve the specificity and sensitivity of the techniques for the diagnosis of human anisakidosis, a method of affinity chromatography for the purification of species-specific antigens from anisakis simplex third-stage larvae (l3) has been developed. new zealand rabbits were immunized with a. simplex or ascaris suum antigens or inoculated with toxocara canis embryonated eggs. the igg-specific antibodies were isolated by means of protein a-sepharose cl-4b bead columns. igg anti-anisak ... | 2001 | 11570559 |
| a model for an ascaris muscle cell. | muscle cells in the nematode ascaris suum undergo bouts of oscillation in the behaving worm, these being correlated with progressing waves of contraction along the body of the worm. the bouts have three time scales: a rough period of 7-20 s, 10-20 short bursts of spikes within a bout, and three to eight spikes per burst. this paper has two aims: showing that there is consistency between measurements of individual currents and measurements on whole muscle cells, and creating a building block to e ... | 2001 | 11571482 |
| chickens and pigs as transport hosts for ascaris, trichuris and oesophagostomum eggs. | ten chickens and 2 pigs were fed non-embryonated eggs of ascaris suum, trichuris suis and oesophagostomum dentatum. each chicken was fed approximately 15,000 eggs of each parasite species while approximately 300,000 eggs were given to each of the pigs. after passage in chickens 8.3% of o. dentatum eggs were recovered in faeces compared to 61.1% and 38.4%, 49.1% and 30.3%, 41.6% of a. suum and t. suis eggs, respectively. after passage in pigs the percentages were respectively. after embryonation ... | 2001 | 11578097 |
| ecological influences on transmission rates of ascaris suum to pigs on pastures. | a study was conducted to determine the distribution and transmission rate of ascaris suum eggs and oesophagostomum dentatum larvae in a pasture/pig house facility, which during the preceding summer was contaminated with helminth eggs by infected pigs. in may, four groups of 10 helminth naïve tracer pigs were exposed to fenced sections of the facility for 7 days and necropsied for parasite recovery 9-10 days later (trial 1). the highest rate of a. suum transmission (201 eggs per day) occurred in ... | 2001 | 11587843 |
| natural antibodies to nematode biotinyl-enzymes in human sera. | biotinyl-enzymes are conservative molecules present in helminths, as well as in other animals, bacteria and plants. they have recently been found to be antigenic in mice, and a potential source of cross-reactivity among helminths. this study investigated the presence in human sera of antibodies reactive with biotinyl-enzymes from the nematodes anisakis simplex, toxocara canis and ascaris suum. biotinyl-enzymes from all these nematodes were recognized by igg1 antibodies in sera from healthy subje ... | 2001 | 11599787 |
| the effect of a bradykinin b2 receptor antagonist, npc-567, on allergen-induced airway responses in a porcine model. | in order to assess the effect of selective blocking of the bradykinin (bk) b2 receptor in allergic airway reactions, the bk b2 receptor antagonist npc-567 was administered to sensitized pigs before allergen challenge. | 2001 | 11603850 |
| cuticular collagen synthesis by ascaris suum during development from the third to fourth larval stage: identification of a potential chemotherapeutic agent with a novel mechanism of action. | the dominant proteins released by ascaris suum during development in vitro from the l3 to l4 stage were identified as collagenous cuticular proteins by sequence analysis and susceptibility to digestion by collagenase. under reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), the collagen proteins separated into 3 groups with molecular weights estimated at 32 kda, 54-60 kda, and 71-91 kda. the 32-kda protein represents monomeric collagen; the 54-60- and 71-91-kda compon ... | 2001 | 11695381 |
| selective protective effects of nitric oxide inhalation on allergen-induced acute airway reactions in the pig. | nitric oxide (no) is thought to be an important mediator of inflammatory processes during allergic reactions in the respiratory tract. | 2001 | 11696056 |
| intranasal immunization with recombinant ascaris suum 14-kilodalton antigen coupled with cholera toxin b subunit induces protective immunity to a. suum infection in mice. | animals can be rendered immune to ascaris parasites by immunization with infectious-stage larvae. the specific parasite gene products that mediate protective responses in ascariasis are unknown. we have identified a cdna encoding ascaris suum 14-kda antigen (as14) and evaluated the vaccinal effect of the escherichia coli-expressed recombinant protein (ras14). genbank analysis showed that as14 has low similarity at the amino acid level to a caenorhabditis elegans gene product and to antigens of t ... | 2001 | 11705899 |
| marginal vitamin a deficiency in pigs experimentally infected with trichuris suis: a model for vitamin a inadequacy in children. | the development of an experimental model for marginal vitamin a deficiency in humans is of major interest, enabling the elucidation of possible interactions with helminth infections. we established a useful experimental model for human vitamin a deficiency in young pigs; deficiency was induced through a depletion method encompassing both sow and offspring. we report on a 2 x 2 study in which 18-week-old vitamin a deficient pigs and vitamin a sufficient littermates were infected with both of the ... | 2001 | 11706673 |
| inactivation of viable ascaris eggs by reagents during enumeration. | various reagents commonly used to enumerate viable helminth eggs from wastewater and sludge were evaluated for their potential to inactivate ascaris eggs under typical laboratory conditions. two methods were used to enumerate indigenous ascaris eggs from sludge samples. all steps in the methods were the same except that in method i a phase extraction step with acid-alcohol (35% ethanol in 0.1 n h(2)so(4)) and diethyl ether was used whereas in method ii the extraction step was avoided by pouring ... | 2001 | 11722892 |
| ex vivo diffusion of albendazole and its sulfoxide metabolite into ascaris suum and fasciola hepatica. | the current experiments compare the pattern of ex vivo uptake (diffusion) of albendazole (abz) and albendazole sulfoxide (abzso) by ascaris suum and fasciola hepatica. specimens of a. suum and f. hepatica were collected from untreated animals (pigs and sheep, respectively) and incubated with either abz or abzso for different time periods (5-180 min). after incubation. the parasite material was analysed by hplc to quantify the amount of abz and/or abzso. the parent drug and its active abzso metab ... | 2001 | 11728018 |
| [two cases of visceral larva migrans due to ascaris suum showing a migratory nodular shadow]. | the number of cases of visceral larva migrans caused by the pig ascarid, ascaris suum has recently been increasing. we have encountered two cases of visceral larva migrans due to a. suum with a nodular shadow on the chest radiograph and eosinophilia in the peripheral blood. patient 1 was a 26-year-old man who had been admitted to our hospital for an elective minor operation. his chest radiology and chest computed tomography revealed a nodule in the left lung field. peripheral blood eosinophil co ... | 2001 | 11729695 |
| extracts of ascaris suum egg and adult worm share similar immunosuppressive properties. | adult ascaris suum body extract (asc) prepared from male and female worms (with stored eggs) down-regulates the specific immune response of dba/2 mice to ovalbumin (oa) and preferentially stimulates a th2 response to its own components, which is responsible for the suppression of the oa-specific th1 response. here, we investigated the participation of soluble extracts prepared from male or female worms or from eggs (e-asc) in these immunological events. extracts from either sex (1 mg/animal) or ... | 2002 | 11743619 |
| controlling ligand binding in myoglobin by mutagenesis. | a quadruple mutant of sperm whale myoglobin was constructed to mimic the structure found in ascaris suum hemoglobin. the replacements include his(e7)-->gln, leu(b10)-->tyr, thr(e10)--> arg, and ile(g8)-->phe. single, double, and triple mutants were characterized to dissect out the effects of the individual substitutions. the crystal structures of the deoxy and oxy forms of the quadruple mutant were determined and compared with that of native ascaris hemoglobin. tyr(b10) myoglobin displays low o( ... | 2002 | 11744723 |
| molecular characterization of a glyceraldehyde-3-phosphate dehydrogenase from the swine roundworm ascaris suum. | | 2002 | 11755196 |
| helminths of wild boar in spain. | forty-seven wild boars (sus scrofa) collected from two areas in the province of valencia (eastern spain) were examined for digestive and respiratory helminth parasites. helminth species and their prevalence were taenia hydatigena cysticercus (19%), ascarops strongylina (87%), physocephalus sexalatus (6%), ascaris suum (2%), metastrongylus spp. (85%), capillaria sp. (2%) and macracanthorhynchus hirudinaceus (21%). trichinella spp. were not found. physocephalus sexalatus, a. suum, capillaria sp. a ... | 2001 | 11763752 |
| [a case of myelitis caused by visceral larva migrans due to ascaris suum presenting only with lhermitte's sign]. | a 27-year-old woman was admitted because of pain radiating through her back on neck flexion that had begun a month ago. she frequently ate raw beef liver. general physical examination revealed no abnormal findings, but she showed lhermitte's sign neurologically. fecal examination revealed no worm eggs. blood cell counts showed mild eosinophilia (8.2%). the ige level was mildly increased to 397 iu/ml (normal < 250). cerebrospinal fluid examination showed 7 cells/microliter with 50% eosinophils. a ... | 2001 | 11771161 |
| abf-1 and abf-2, asabf-type antimicrobial peptide genes in caenorhabditis elegans. | two genes encoding the asabf (ascaris suum antibacterial factor)-type antimicrobial peptide, abf-1 and abf-2, were identified in caenorhabditis elegans. recombinant abf-2 exhibited potent microbicidal activity against gram-positive and gram-negative bacteria, and yeasts. the tissue-specific distribution estimated by immunofluorescence staining and transgenic analysis of a gfp fusion gene (where gfp corresponds to green fluorescent protein) suggested that abf-2 contributes to surface defence in t ... | 2002 | 11772394 |
| role of complex ii in anaerobic respiration of the parasite mitochondria from ascaris suum and plasmodium falciparum. | parasites have developed a variety of physiological functions necessary for existence within the specialized environment of the host. regarding energy metabolism, which is an essential factor for survival, parasites adapt to low oxygen tension in host mammals using metabolic systems that are very different from that of the host. the majority of parasites do not use the oxygen available within the host, but employ systems other than oxidative phosphorylation for atp synthesis. in addition, all pa ... | 2002 | 11803022 |
| [adaptation to low oxygen tension in parasite mitochondria]. | | 2002 | 11808193 |
| the mitochondrial genomes of the human hookworms, ancylostoma duodenale and necator americanus (nematoda: secernentea). | the complete mitochondrial genome sequences were determined for two species of human hookworms, ancylostoma duodenale (13,721 bp) and necator americanus (13,604 bp). the circular hookworm genomes are amongst the smallest reported to date for any metazoan organism. their relatively small size relates mainly to a reduced length in the at-rich region. both hookworm genomes encode 12 protein, two ribosomal rna and 22 transfer rna genes, but lack the atp synthetase subunit 8 gene, which is consistent ... | 2002 | 11812491 |
| purification and characterization of alpha-amylases from the intestine and muscle of ascaris suum (nematoda). | alpha-amylase (ec 3.2.1.1) was purified from the muscle and intestine of the parasitic helminth of pigs ascaris suum. the enzymes from the two sources differed in their properties. isoelectric focusing revealed one form of a-amylase from muscles with pl of 5.0, and two forms of amylase from intestine with pi of 4.7 and 4.5. sds/page suggested a molecular mass of 83 kda and 73 kda for isoenzymes of a-amylases from intestine and 59 kda for the muscle enzyme. alpha-amylase from intestine showed max ... | 2001 | 11833785 |
| how nematode sperm crawl. | sperm of the nematode, ascaris suum, crawl using lamellipodial protrusion, adhesion and retraction, a process analogous to the amoeboid motility of other eukaryotic cells. however, rather than employing an actin cytoskeleton to generate locomotion, nematode sperm use the major sperm protein (msp). moreover, nematode sperm lack detectable molecular motors or the battery of actin-binding proteins that characterize actin-based motility. the ascaris system provides a simple 'stripped down' version o ... | 2002 | 11839788 |
| [epidemiological study on the influence of pig-derived ascaris to the transmission of human ascariasis]. | an experimental epidemiological study was designed to investigate possible relations of pig-derived ascaris to the transmission of human ascariasis which might be caused by cross-infection between the pig and humans. | 2001 | 11860859 |
| new telomere formation during the process of chromatin diminution in ascaris suum. | chromatin diminution in the parasitic nematode ascaris suum represents an interesting case of developmentally programmed dna rearrangement in higher eukaryotes. at the molecular level, it is a rather complex event including chromosome breakage, new telomere formation and dna degradation. analysis of a cloned somatic telomere (ptel1) revealed that it has been newly created during the process of chromatin diminution by the addition of telomeric repeats (ttaggc)n to a chromosomal breakage site (mül ... | 2002 | 11902675 |
| the minimal trna: unique structure of ascaris suum mitochondrial trna(ser)(ucu) having a short t arm and lacking the entire d arm. | the tertiary structure of ascaris suum mitochondrial trna(ser)(ucu) was examined by nuclear magnetic resonance analysis using its transcript, since trna(ser)(ucu), lacking the d arm and possessing a truncated t arm, is the shortest of all the known trnas. most basepairs in the proposed secondary structure of trna(ser)(ucu) were shown to exist, but the connector region comprising the truncated d loop and the extra loop was flexible. this flexibility, would enable adjustment of the mutual distance ... | 2002 | 11904178 |
| [zafirlukast (accolate): a review of its pharmacological and clinical profile]. | today, bronchial asthma is considered as a chronic inflammatory disease of the airway. it has been revealed that various chemical mediators are involved in the onset of bronchial asthma. among them, particularly, the peptide leukotrienes, ltc4, ltd4 and lte4, have been known to play a pathophysiological important role in asthma. zafirlukast binds to the cyslt1 receptors competitively with these peptide leukotrienes and inhibited peptide leukotriene-induced constriction of isolated guinea pig tra ... | 2002 | 11979731 |
| enantioselective binding of albendazole sulphoxide to cytosolic proteins from helminth parasites. | the pharmacological effect of the active albendazole metabolite, albendazole sulphoxide (abzso), depends on its sustained presence at the site of parasite location and its binding to helmith beta-tubulin. abzso is found in the plasma and tissues of albendazole-treated animals in two enantiomeric forms: (+)abzso and (-)abzso. knowledge of enantioselectivity in drug action is necessary, since any difference in target proteins affinity between enantiomers may have implications on the pharmacologica ... | 2002 | 11980387 |
| evaluation of cell-surface ige receptors on the canine mastocytoma cell line c2 maintained in continuous culture. | to assess expression and function of cell-surface ige receptors on the canine mastocytoma cell line c2 maintained in continuous culture. | 2002 | 12013481 |
| evaluation by elisa of anisakis simplex larval antigen purified by affinity chromatography. | in order to improve the specificity and sensitivity of the techniques for the human anisakidosis diagnosis, a method of affinity chromatography for the purification of species-specific antigens from anisakis simplex third-stage larvae (l3) has been developed. new zealand rabbits were immunized with a. simplex or ascaris suum antigens or inoculated with toxocara canis embryonated eggs. the igg specific antibodies were isolated by means of protein a-sepharose cl-4b beads columns. igg anti-a. simpl ... | 2002 | 12016451 |
| how helminth lipid-binding proteins offload their ligands to membranes: differential mechanisms of fatty acid transfer by the aba-1 polyprotein allergen and ov-far-1 proteins of nematodes and sj-fabpc of schistosomes. | three different classes of small lipid-binding protein (lbp) are found in helminth parasites. although of similar size, the aba-1a1 (also designated as-npa-a1) and ov-far-1 (formerly known as ov20) proteins of nematodes are mainly alpha-helical and have no known structural counterparts in mammals, whereas sj-fabpc of schistosomes is predicted to form a beta-barrel structure similar to the mammalian family of intracellular fatty acid binding proteins. the parasites that produce these proteins are ... | 2002 | 12022874 |
| crystal structure of the malic enzyme from ascaris suum complexed with nicotinamide adenine dinucleotide at 2.3 a resolution. | the structure of the ascaris suum mitochondrial nad-malic enzyme in binary complex with nad has been solved to a resolution of 2.3 a by x-ray crystallography. the structure resembles that of the human mitochondrial enzyme determined in complex with nad [xu, y., bhargava, g., wu, h., loeber, g., and tong, l. (1999) structure 7, 877-889]. the enzyme is a tetramer comprised of subunits possessing four domains organized in an "open" structure typical of the nad-bound form. the subunit organization, ... | 2002 | 12033925 |
| synthetic studies on glycosphingolipids from protostomia phyla: synthesis of amphoteric glycolipid analogues from the porcine nematode, ascaris suum. | a novel amphoteric glycosphingolipid, cholinephosphoryl-(-->6)-beta-d-glcpnac-(1-->3)-beta-d-manp-(1-->4)-beta-d-glcp-(1-->)-cer, isolated from the porcine parasitic nematode, ascaris suum, may be expected to be involved in host-parasite interactions. this glycosphingolipid analogue containing octyl residue in place of ceramide was synthesized as follows: the key reaction of this synthetic procedure is the formation of a intramolecular aglycon delivery (iad) approach for beta-selective mannosyla ... | 2002 | 12036012 |
| the tryptase inhibitor apc-366 reduces the acute airway response to allergen in pigs sensitized to ascaris suum. | tryptase is a mast cell serine protease that is released during mast cell degranulation. it has been implicated as an important enzyme in the pathophysiology of asthma, but its role in this disease is not fully elucidated. | 2002 | 12047446 |
| impact of protein energy malnutrition on trichuris suis infection in pigs concomitantly infected with ascaris suum. | the objective of this experiment was to investigate a possible interaction between protein and energy malnutrition (pem) and intestinal nematode infections. we report on a 3 x 2 factorial study in which pigs were fed either a low protein energy (lpe), low protein (lp) or a normal protein energy (npe) diet, and 6 weeks later inoculated with trichuris suis (4000 infective eggs). secondarily, in order to obtain a polyparasitic status, pigs were concomitantly inoculated with ascaris suum (600 infect ... | 2002 | 12049419 |
| microarray profile of differentially expressed genes in a monkey model of allergic asthma. | inhalation of ascaris suum antigen by allergic monkeys causes an immediate bronchoconstriction and delayed allergic reaction, including a pulmonary inflammatory infiltrate. to identify genes involved in this process, the gene-expression pattern of allergic monkey lungs was profiled by microarrays. monkeys were challenged by inhalation of a. suum antigen or given interleukin-4 (il-4) treatment; lung tissue was collected at 4, 18 or 24 h after antigen challenge or 24 h after il-4. each challenged ... | 2002 | 12049661 |
| 2.6 a resolution crystal structure of helices of the motile major sperm protein (msp) of caenorhabditis elegans. | the amoeboid locomotion of nematode sperm is mediated by the assembly dynamics of the major sperm protein (msp). msp forms fibrous networks based on a hierarchy of macromolecular assemblies: helical subfilaments are built from msp dimers; filaments are formed from two subfilaments coiling round one another; and filaments themselves supercoil to produce bundles. to provide a structural context for understanding the role of these macromolecular assemblies in cell locomotion, we have determined the ... | 2002 | 12051923 |
| alternative migration routes of ascaris suum in the pig. | experiments were conducted to investigate possible alternative routes of extraintestinal migration of ascaris suum larvae in the pig. pigs were infected with a. suum via injection of newly hatched larvae into cecal veins (i.v.), into cecal lymph nodes (ln), or intraperitoneally (i.p.), and control animals were inoculated orally with infective eggs (p.o.). two pigs per inoculation route were necropsied on days 1, 4, and 13 postinoculation. the numbers of liver lesions and the percentage of larvae ... | 2002 | 12053960 |
| helminth infections in danish organic swine herds. | in nine organic swine herds, faecal excretion and pasture contamination by parasite eggs/larvae were studied in a period from march to october 1999. it was shown that the organic pigs were infected with ascaris suum (28% of weaners, 33% of fatteners, 4% of sows), trichuris suis (4% of weaners, 13% of fatteners, <1% of sows) and oesophagostomum spp. (5% of weaners, 14% of fatteners, 20% of sows) whereas no infections with hyostrongylus rubidus, metastrongylus spp. or strongyloides ransomi were de ... | 2002 | 12062513 |
| pharmacology of n-(3,5-dichloro-1-oxido-4-pyridinyl)-8-methoxy-2-(trifluoromethyl)-5-quinoline carboxamide (sch 351591), a novel, orally active phosphodiesterase 4 inhibitor. | n-(3,5-dichloro-1-oxido-4-pyridinyl)-8-methoxy-2-(trifluoromethyl)-5-quinoline carboxamide (sch 351591) has been identified as a potent (ic(50) = 58 nm) and highly selective type 4 phosphodiesterase (pde4) inhibitor with oral bioactivity in several animal models of lung inflammation. n-(3,5-dichloro-4-pyridinyl)-8-methoxy-2-(trifluoromethyl)-5-quinoline carboxamide (sch 365351), the only significant in vivo metabolite, is also a potent and highly selective pde4 inhibitor (ic(50) = 20 nm). both s ... | 2002 | 12065709 |
| a cathepsin l protease essential for caenorhabditis elegans embryogenesis is functionally conserved in parasitic nematodes. | proteolytic enzymes are involved in processes important to development and survival of many organisms. parasite proteases are considered potential targets of parasite control yet, for most, their precise physiological functions are unknown. validation of potential targets requires analysis of function. we have recently identified a cathepsin l (cpl) cysteine protease, ce-cpl-1, which is essential for embryonic development of the free-living nematode caenorhabditis elegans. we now show that cpl g ... | 2002 | 12076767 |
| [sequencing and homology analysis of cdnas encoding fhgst and asgst]. | to study the homologies between fhgst, asgst and sj26gst. | 1998 | 12078253 |
| the time-course of the response to the fmrfamide-related peptide pf4 in ascaris suum muscle cells indicates direct gating of a chloride ion-channel. | we investigated the effects of pf4 on ascaris suum somatic muscle cells using a 2 electrode current-clamp technique. pf4 is a farp (fmrfamide-related peptide), originally isolated from the free-living nematode panagrellus redivivus. pf4 caused hyperpolarization and an increase in chloride ion conductance when it was applied to the muscle cells of the ascaris body wall. the delay between the application of the peptide and the appearance of the response was measured and compared with that of gamma ... | 2002 | 12118721 |
| role of cysteine 306 in the catalytic mechanism of ascaris suum phosphoenolpyruvate carboxykinase. | biochemical and metabolic data lead to the conclusion that the enzyme phosphoenolpyruvate carboxykinase (pepck) contributes to a critical point of divergence in energy conservation pathways between mammals and nematodes. the ascaris suum pepck shares considerable homology with pepck from avian liver and is a good candidate for mutagenesis studies. the cys306 substitution by ser and ala produced active enzymes and the two mutants are kinetically indistinguishable from each other. this substitutio ... | 2002 | 12127066 |
| visceral larva migrans due to ascaris suum which presented with eosinophilic pneumonia and multiple intra-hepatic lesions with severe eosinophil infiltration--outbreak in a japanese area other than kyushu. | a 32-year-old man presented with the chief complaint of severe cough. examination of peripheral blood showed a marked increase in eosinophils. chest ct demonstrated multiple ground glass opacities in both lungs. bronchoalveolar lavage showed abundant eosinophils. abdominal ct demonstrated multiple low attenuation areas in the liver. liver biopsy with ultrasonography revealed severe eosinophil infiltrations around the portal veins. serologically, a multi-dot enzyme linked immunosorbent assay (dot ... | 2002 | 12132528 |
| purification and characterization of a novel glutathione s-transferase from asaphis dichotoma. | an isoenzyme of glutathione s-transferase (adgst) was purified from liver intestine of the seashell (asaphis dichotoma) by gst-sepharose 4b affinity chromatography followed by reverse-phase hplc. the enzyme has a pi value of 4.6 and is composed of two subunits each with a molecular weight of 23kda. it exhibits different catalytic activities toward the substrates 1-chloro-2,4-dinitrobenzene, 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, ethacrynic acid, and p-nitrophenyl acetate and, fascinatingly, sh ... | 2002 | 12139969 |
| the biological activity of aba-1-like protein from ascaris lumbricoides. | the elevation of non-specific ige (total ige) in ascaris infection can be seen one week after infection, and reaches a peak after approximately two weeks. it has been reported that aba-1 protein is the main constituent in the pseudocoelomic fluid of ascaris suum. to investigate the effect of the aba-1-like protein from ascaris lumbricoides (alb), the cdna was cloned by reverse transcriptase polymerase chain reaction, using original primers based on the consensus sequences of aba-1 and tba-1, tha ... | 2001 | 12160220 |
| species-specific proteins identified in ascaris lumbricoides and ascaris suum using two-dimensional electrophoresis. | the protein profile of adult female ascaris lumbricoides and ascaris suum originating from humans and pigs, respectively, was studied using two-dimensional polyacrylamide gel electrophoresis. six different major protein spots specific for a. lumbricoides were identified irrespective of their geographical origin and no major specific spot was encountered in a. suum. no major differences in the protein profiles between the extract by phosphate-buffered saline and urea were encountered for either a ... | 2002 | 12172821 |
| effects of an eosinophil chemotaxis inhibitor, tak-661, on antigen-induced asthmatic responses in allergic sheep. | eosinophils have been shown to play a role in allergen-induced airway responses. the aim in this study was to examine the effects of tak-661, a newly developed product as a specific inhibitory agent of eosinophil chemotaxis, on antigen-induced asthmatic responses in allergic sheep model. seven ascaris-sensitive, "dual-respondent" allergic sheep were provocated by an ascaris suum antigen or phosphate-buffered saline 2 hrs after intra-stomach administration of tak-661 or a placebo. pulmonary resis ... | 2002 | 12177730 |
| eosionophilic pseudotumor of the liver due to ascaris suum infection. | a case of eosinophilic pseudotumor of the liver due to ascaris (a) suum is described in a 34-year-old-man with a high serum level of immunoglobulin e and hypereosinophilia ascribed to a history of atopic dermatitis since childhood. multiple hepatic hypoechoic nodules detected by ultrasound were confirmed as low-density nodules on computed tomography (ct), and as low and high signal intensity lesions on t1-and t2-weighted magnetic resonance imaging (mri), respectively. ct during arteriography (ct ... | 2002 | 12191679 |
| lung-stage protein profile and antigenic relationship between ascaris lumbricoides and ascaris suum. | the protein profile and antigenic properties of lung-stage larvae of ascaris lumbricoides and a. suum were studied using 2-dimensional electrophoresis and immunoblot analysis, respectively. the protein profiles of the 2 parasites were identical except for the presence of only 1 major protein spot specific for each. there was a complete cross-reactivity between the 2 parasites at the immunological level, and no specific antigen was recognized using specific antibody raised against the 2 parasites ... | 2002 | 12197147 |
| bioactivity studies on beta-sitosterol and its glucoside. | beta-sitosterol and beta-sitosteryl-beta-d-glucoside were isolated as analgesic constituents from the leaves of mentha cordifolia opiz. the acetic acid-induced writhing test showed that beta-sitosterol and beta-sitosteryl-beta-d-glucoside decreased the number of squirms induced by acetic acid by 70.0% and 73.0%, respectively, at a dose of 100 mg / kg mouse. statistical analysis using the kruskall wallis one-way analysis of variance by ranks showed that these isolates approximate the analgesic ac ... | 2002 | 12203259 |
| anthelmintic paraherquamides are cholinergic antagonists in gastrointestinal nematodes and mammals. | oxindole alkaloids in the paraherquamide/marcfortine family exhibit broad-spectrum anthelmintic activity that includes drug-resistant strains of nematodes. paraherquamide (phq), 2-deoxoparaherquamide (2dphq), and close structural analogs of these compounds rapidly induce flaccid paralysis in parasitic nematodes in vitro, without affecting adenosine triphosphate (atp) levels. the mechanism of action of this anthelmintic class was investigated using muscle tension and microelectrode recording tech ... | 2002 | 12213111 |
| structural elucidation of zwitterionic carbohydrates derived from glycosphingolipids of the porcine parasitic nematode ascaris suum. | carbohydrates substituted with phosphocholine (pc) and phosphoethanolamine (pe) were released from zwitterionic glycosphingolipids of the pig parasitic nematode ascaris suum by treatment with endoglycoceramidase. individual glycans were obtained by hplc on porous graphitic carbon followed by high-ph anion-exchange chromatography. in addition to the known pentasaccharides gal alpha 3galnac beta 4[pc6]glcnac beta 3man beta 4glc and gal alpha 3galnac beta 4[pc6]glcnac beta 3[pe6]man beta 4glc, the ... | 2003 | 12234251 |
| electron-transfer complexes in ascaris mitochondria. | parasites have developed a variety of physiological functions necessary for their survival within the specialized environment of the host. using metabolic systems that are very different from those of the host, they can adapt to low oxygen tension present within the host animals. most parasites do not use the oxygen available within the host to generate atp, but rather employ anaerobic metabolic pathways. in addition, all parasites have a life cycle. in many cases, the parasite employs aerobic m ... | 2002 | 12238891 |
| alternative substrates for malic enzyme: oxidative decarboxylation of l-aspartate. | the nad-malic enzyme from ascaris suum will utilize l-aspartate, (2s,3r)-tartrate, and meso-tartrate as substrates with v/k values 10(-4)-10(-5) with respect to malate. there is a strict requirement for the 2s stereochemistry for all of these reactants. since aspartate is unique as an amino acid reactant for malic enzyme, it was informative to determine the details of its mechanism of oxidative decarboxylation. the initial rate of nadh appearance is directly proportional to the concentration of ... | 2002 | 12356322 |
| modulation of a heterologous immune response by the products of ascaris suum. | helminth infections are among the most potent stimulators of th2-type immune responses and have been widely demonstrated to modify responsiveness to both nonparasite antigens and other infectious agents in a nonspecific manner in infected animals. we investigated the immunomodulatory properties of pseudocoelomic body fluid from adult ascaris suum gastrointestinal helminths (abf) and its defined allergen (aba-1) by examining their effects on the immune response to a heterologous antigen, ovalbumi ... | 2002 | 12379682 |
| endothelin-induced vascular and bronchial effects in pig airways: role in acute allergic responses. | the effects of endothelin (et) agonists on airway mechanics and bronchial blood flow were studied as well as the effects of mixed et-receptor antagonist bosentan on allergen-induced airway reactions in the pig. et agonists [et-1, et-3, and the et(b) receptor-selective agonist sarafotoxin 6c (sf6c)] were given as intravenous injections (0.4-200 pmol/kg) to eight anesthetized pigs. bosentan (10 mg/kg iv) was then administered, and the injections were repeated. only sf6c caused a significant increa ... | 2002 | 12381744 |
| an embryo-associated fatty acid-binding protein in the filarial nematode brugia malayi. | brugia malayi is a filarial nematode parasite that causes lymphatic filariasis, a disease that affects millions of people in the tropics. sexual reproduction of filarial worms occurs within the lymphatic vessels of the human host and is crucial for transmission of the parasite to the mosquito vector. we have previously identified several b. malayi genes that exhibit apparent gender-specific expression. one of these had significant sequence similarity to the ascaris suum embryo-associated fatty a ... | 2002 | 12387845 |
| secretion of a novel developmentally regulated chitinase (family 19 glycosyl hydrolase) into the perivitelline fluid of the parasitic nematode, ascaris suum. | the early development of the parasitic nematode, ascaris suum, occurs within a chitinous eggshell and an abundant chitinase (as-p50) has been identified in the perivitelline fluid (pvf) surrounding the infective larva prior to hatching. a cdna encoding as-p50 was cloned, sequenced and the protein expressed in escherichia coli. as-p50 is a member of glycosyl hydrolase family 19, previously identified only in plants, making the characterization of as-p50 the first family 19 glycosyl hydrolase from ... | 2002 | 12387846 |
| haemoconcentration associated with low vitamin a status can mask anaemia. | as part of a pig study to elucidate the interactions between low vitamin a status and helminth infections, surprisingly, we observed higher haemoglobin levels and packed cell volumes in the pigs with low vitamin a status. a possible haemoconcentration effect, due to some disturbance in the regulation of the extracellular fluid volume, could lead to underestimation of the prevalence of anaemia in vitamin a deficient human populations. therefore, this phenomenon needs to be further clarified in st ... | 2002 | 12387911 |
| montelukast prevents antigen-induced mucociliary dysfunction in sheep. | the cysteinyl leukotrienes are potent proinflammatory mediators that, in addition to their bronchospastic actions, can also contribute to mucociliary dysfunction, a central component of the pathophysiology of asthma. in this study, we determined whether montelukast, a cysteinyl leukotriene 1 receptor antagonist, could prevent and/or reverse antigen-induced mucociliary dysfunction in allergic sheep. we measured tracheal mucus velocity, a marker of mucociliary clearance, before and for 8 hours aft ... | 2002 | 12406819 |
| functional characterization of alternatively spliced 5-ht2 receptor isoforms from the pharynx and muscle of the parasitic nematode, ascaris suum. | serotonin (5-ht) receptors play key regulatory roles in nematodes and alternatively spliced 5-ht2 receptor isoforms have been identified in the parasitic nematode, ascaris suum. 5-ht2as1 and 5-ht2as2 contain different c-termini, and 5-ht2as1delta4 lacks 42 amino acids at the c-terminus of the third intracellular loop. 5-ht2as1 and 5-ht2as2 exhibited identical pharmacological profiles when stably expressed in human embryonic kidney (hek) 293 cells. both 5-ht2as isoforms had higher affinity for 5- ... | 2002 | 12423236 |
| characterization of sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and western blotting analysis. | sm14 was the first fatty acid-binding protein homologue identified in helminths. thereafter, members of the same family were identified in several helminth species, with high aminoacid sequence homology between them. in addition, immune crossprotection was also reported against fasciola hepatica infection, in animals previously immunized with the schistosoma mansoni vaccine candidate, r-sm14. in the present study, data on preliminary sodium dodecyl sulphate-polyacrylamide gel electrophoresis and ... | 2002 | 12426606 |
| antimicrobial peptides and protease inhibitors in the skin secretions of the crawfish frog, rana areolata. | the dorsal skin of the crawfish frog, rana areolata, is associated with numerous prominent granular glands. proteomic analysis of electrically stimulated skin secretions from these glands enabled the identification and characterization of eight peptides with antimicrobial and hemolytic activity belonging to the previously identified brevinin-1, temporin-1, palustrin-2, palustrin-3, esculentin-1 (two peptides), and ranatuerin-2 (two peptides) families. the primary structures of the peptides were ... | 2002 | 12429503 |
| minor participation of camp on the protein kinase phosphorylation of mitochondrial and cytosolic fractions from ascaris suum: a comparative study with porcine heart muscle. | in contrast to porcine heart muscle in which camp effectively activated the phosphorylation of cytosolic proteins, camp exerted a minor effect on the phosphorylation of proteins from the soluble fraction of ascaris suum muscle. similarly, camp did not enhance the kinase activity in the mitochondrial membranes from porcine heart and a. suum, although major differences in protein phosphorylation were observed between both fractions. however, camp-dependent protein kinases (pka) were evidenced in t ... | 2002 | 12431408 |
| levamisole receptor phosphorylation: effects of kinase antagonists on membrane potential responses in ascaris suum suggest that cam kinase and tyrosine kinase regulate sensitivity to levamisole. | a two-micropipette current-clamp technique was used to record electrophysiological responses from the somatic muscle of ascaris suum. levamisole and acetylcholine were applied to the bag region of the muscle using a microperfusion system. depolarizations produced by 10 s applications of 10 micro mol l(-1) levamisole or 20 s applications of 10 micro mol l(-1) acetylcholine were recorded. the effect on the peak membrane potential change of the kinase antagonists h-7, staurosporine, kn-93 and genis ... | 2002 | 12432019 |
| synthetic studies on glycosphingolipids from the protostomia phyla: syntheses of arthro-series glycosphingolipids. | glycosphingolipids isolated from larvae of the green-bottle fly, lucilia caesar, have quite unique structures containing glcnacbeta-(1 --> 3)-man and galnacbeta-(1 --> 4)-glcnacbeta-(1 --> 3)-man. we have synthesized two glycosphingolipids, beta-d-glcnacp-(1 --> 3)-beta-d-manp-(1 --> 4)-beta-d-glcp-(1 --> 1)-cer and beta-d-galnacp-(1 --> 4)-beta-d-glcnacp-(1 --> 3)-beta-d-manp-(1 --> 4)-beta-d-glcp-(1 --> 1)-cer. a key reaction in the synthetic sequence is the application of the intramolecular a ... | 2002 | 12433469 |
| synthesis and nematocidal activities of n-(2-methylpropyl)-3-(x-phenyl)-2e-propenamides. | | 2002 | 12434551 |