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histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer diatraea saccharalis (fabricius) (lepidoptera: crambidae).the silk gland in lepidoptera larvae is responsible for the silk production used for shelter or cocoon construction. the secretion of fibroin and sericin by the different silk gland regions are well established. there are few attempts to detect lipid components in the insect silk secretion, although the presence of such element may contribute to the resistance of the shelter to wet environment. this study characterizes the glandular region and detects the presence of lipid components in the secr ...200718060296
morphological aspects of cluster formation in the germarium of the sugarcane borer diatraea saccharalis fabricius (lepidoptera: pyralidae).diatraea saccharalis f. is one of the greatest pests of the sugar cane culture. this report aimed to characterize the germarium region of the sugarcane borer by light and transmission electron microscopy, emphasizing the morphological steps of the ovarian cluster formation. in the germarium of this insect, four zones could be morphologically identified during the cluster formation. in the most apical end of each ovariole--zone i--the germ line stem cells undergo complete mitotic division, origin ...200617144137
resistance to stem borers (lepidoptera: crambidae) among texas rice cultivars.a 4-yr field study was conducted to assess the resistance of rice, oryza sativa l., cultivars to injury from the sugarcane borer, diatraea saccharalis (f.), and the mexican rice borer, eoreuma loftini (dyar) (both lepidoptera: crambidae). several cultivars showed reduced levels of injury ('clxl8', 'xl8', 'wells', 'cheniere', and 'xp710' in 2003; clxl8, xp723, cheniere, and 'cl161' in 2004) and lower stem borer yield loss (clxl8 in 2004) than the more susceptible 'priscilla'. the resistant clxl8 ...200617066824
sugarcane borer (lepidoptera: crambidae) management threshold assessment on four sugarcane cultivars.this research assesses the potential for using different economic injury thresholds in management of a key insect pest on susceptible and resistant commercially produced cultivars of sugarcane (saccharum spp. hybrids). in a 2-yr sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), study involving four commercially produced sugarcane cultivars and four insecticide treatment thresholds, 'lcp 85-384' and 'hocp 91-555' were the most susceptible based on percentage of bored internodes ...200616813338
cowpea (vigna unguiculata) vicilins bind to the peritrophic membrane of larval sugarcane stalk borer (diatraea saccharalis).in this work, we show that vicilins from two vigna unguiculata (cowpea) genotypes, epace-10 and it 81d-1045, which are susceptible and resistant to attack by the cowpea weevil callosobruchus maculatus, respectively, associate with the peritrophic membrane (pm) from larvae of diatraea saccharalis. solutions with increasing concentrations of vicilins were incubated with pm of the larvae and subsequently analysed by electrophoresis with sds. it was observed that the majority of the bands of approxi ...200316256689
reduced susceptibility to tebufenozide in populations of the sugarcane borer (lepidoptera: crambidae) in louisiana.susceptibility of the sugarcane borer, diatraea saccharalis (f.), to tebufenozide was measured using a feeding bioassay, and values obtained were compared with baselines generated before the use of this insecticide in louisiana sugarcane, saccharum spp. results from our study suggest that susceptibility to tebufenozide is decreasing in field-collected sugarcane borers. inflections in the log dosage-probit lines were detected for many of the field collections, indicating increased heterogeneity w ...200516022328
evaluation of lectin-expressing transgenic sugarcane against stalkborers (lepidoptera: pyralidae): effects on life history parameters.the impact of snowdrop lectin (galanthus nivalis agglutinin, gna) expressed in transgenic sugarcane on life history parameters of mexican rice borer [eoreuma loftini (dyar)] and sugarcane borer [diatraea saccharalis (f.)] (both lepidoptera: pyralidae) was evaluated. in the laboratory, lyophilized sugarcane leaf sheath tissue was incorporated in a meridic diet resulting in a gna concentration of 0.47% of total protein, and used for insect bioassays over two successive generations. deleterious eff ...200212020029
selection, egg viability, and fecundity of the sugarcane borer (lepidoptera: crambidae) with tebufenozide.two separate attempts to select the sugarcane borer, diatraea saccharalis (f.), for resistance to tebufenozide were unsuccessful. both selected colonies were lost after the fourth generation due to a lack of oviposition. differences were not detected in fecundity or percent egg viability for 5-d-old third instars exposed to concentrations (ec5, ec15, and ec30) of tebufenozide for 7 d. decreases (p < or = 0.01) in mean female pupal weights were detected in larvae exposed to ec15 and ec30 concentr ...200111777063
susceptibility of diatraea saccharalis (lepidoptera: crambidae) to tebufenozide.dosage-mortality baselines were determined for first and newly molted third instars of the sugarcane borer, diatraea saccharalis (f.), with tebufenozide incorporated into an artificial diet. using death as an endpoint for these assays, lc50 and lc90 values were estimated to be 0.17 and 0.41 ppm for the first and 0.87 and 3.67 ppm for third instars, respectively. developmental effects also were observed and were defined to include mortality as well as physical impairment and retarded or prolonged ...200111777050
sampling for the sugarcane borer (lepidoptera: crambidae) on sugarcane in louisiana.a 3-yr study was conducted in 0.6- to 2.0-ha sugarcane fields throughout south louisiana under varying sugarcane borer, diatraea saccharalis (f.), density levels to determine the spatial dispersion of infestations and to develop a sequential sampling plan. infestations of d. saccharalis were randomly dispersed. infestation levels (percentage of stalks infested) ranged from 0.6 to 33.3%. frequency distributions of the number of infested stalks indicated that the poisson distribution best fit the ...200111425035
mortality staging of dominant lethals induced in the f1 generation of the sugarcane borer; diatraea saccharalis (f.).comparative egg maturation was observed between fertile eggs laid by unirradiated females, by unirradiated females mated with irradiated males, by irradiated females mated with normal males, and by virgin unirradiated females. haploid eggs turn yellow and dry by the fifth day. eggs from irradiated parents develop more slowly than normal eggs. most embryos from parents irradiated as p generation adults at 16.8 kilorads for males and 12.0 kr for females die early in embryonic development. a descri ...196817387934
population dynamics of digitaria spp submitted to selection pressure by herbicides in sugarcane crop.the objective of this research was to study population dynamics of the weed crabgrass, genus digitaria, submitted to selection pressure by herbicides currently applied in sugarcane crops in brazil. in the first experiment two crabgrass species (digitaria nuda and digitaria ciliaris) and eight herbicide treatments applied in preemergence were used, and control percentage was evaluated at 7, 14, and 21 days after herbicide application (daa). in the second experiment the level of tolerance through ...200515656158
effects of tissue type and promoter strength on transient gus expression in sugarcane following particle bombardment.effects of tissue type and promoter strength on transient gus expression in the sugarcane (saccharum spp. hybrids) cultivar nco 310 were evaluated following microprojectile bombardment of leaf explants. gus expression was histochemically or fluorometrically measured 48 h after delivery of the uida gene. high levels of gus expression were obtained in leaf segments isolated from young, expanding sugarcane leaves cultured for 1, 3, or 6 d prior to bombardment. the promoter derived from the maize ub ...199324201961
effectiveness of attract-and-kill systems using methyl eugenol incorporated with neonicotinoid insecticides against the oriental fruit fly (diptera: tephritidae).laboratory bioassays and field trials were conducted to evaluate an "attract-and-kill" system using methyl eugenol (me) with neonicotinoid insecticides against male oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae). in laboratory bioassays, mortality of male flies resulting from the conventional toxicant, naled was 98.3-100% at 24 through 72 h after treatment, whereas the neonicotinoid insecticides imidacloprid and acetamiprid caused only approximately 60-80% at 24 through ...200818459398
vermistabilization of municipal sewage sludge amended with sugarcane trash using epigeic eisenia fetida (oligochaeta).efforts have been made in this study to stabilize the sewage sludge mixed with sugarcane trash in four different proportions: 20% (t(1)); 40% (t(2)); 60% (t(3)) and 80% (t(4)), under laboratory conditions using epigeic earthworm (oligochaeta) eisenia fetida. the composting potential of worm was also evaluated in 100% sewage sludge treatment (t(5)). the changes in chemical properties of substrate was measured at the end. the vermicomposted material showed decrease in organic c (4.8-12.7%) and exc ...200918672324
silicon reduces impact of plant nitrogen in promoting stalk borer (eldana saccharina) but not sugarcane thrips (fulmekiola serrata) infestations in sugarcane.the stalk borer eldana saccharina walker (lepidoptera: pyralidae) is a major limiting factor in south african sugarcane production, while yield is also reduced by sugarcane thrips fulmekiola serrata kobus (thysanoptera: thripidae). borer management options include appropriate nitrogen (n) and enhanced silicon (si) nutrition; the effect of n on sugarcane thrips is unknown. we tested the effects of these nutrients, in combination with resistant (n33) and susceptible (n27) sugarcane cultivars, on e ...201424999349
population genetics of eldana saccharina walker (lepidoptera: pyralidae) and the implications for management using biocontrol.the african sugarcane stalk borer, eldona saccharina walker (lepidoptera: pyralidae), is widely distributed throughout sub-saharan africa and is an important insect pest of maize and sugarcane. the insect shows significant variation in behaviour, host plant and natural enemy guild in different regions. several attempts to redistribute the natural enemies of e. saccharina from west africa to south africa were unsuccessful. the significant behavioural, host plant and natural enemy variations as we ...201021539263
within-field damage and distribution patterns of the stalk borer, eldana saccharina (lepidoptera: pyralidae), in sugarcane and a comparison with nematode damage.the occurrence of eldana saccharina (lepidoptera: pyralidae) was monitored in grids represented by plots in 12 nematicide trials in south african sugarcane fields. the trials encompassed a total of eight plant cane crops and 22 ratoon crops and were situated within commercial cane fields. several measurements were made to characterize the damage caused by e. saccharina. these included the number of internodes per stalk, the percentage of internodes damaged and the percentage of stalks damaged. t ...201019860988
water stress augments silicon-mediated resistance of susceptible sugarcane cultivars to the stalk borer eldana saccharina (lepidoptera: pyralidae).silicon (si) can improve resistance of plants to insect attack and may also enhance tolerance of water stress. this study tested if si-mediated host plant resistance to insect attack was augmented by water stress. four sugarcane cultivars, two resistant (n21, n33) and two susceptible (n26, n11) to eldana saccharina walker were grown in a pot trial in si-deficient river sand, with (si+) and without (si-) calcium silicate. to induce water stress, irrigation to half the trial was reduced after 8.5 ...200717411480
desymmetrization of 1,4-dien-3-ols and related compounds via ueno-stork radical cyclizations[formula: see text] desymmetrization of 1,4-dien-3-ols and related compounds via ueno-stork radical cyclizations is reported. the stereochemistry of the cyclization is controlled by the acetal center. excellent stereocontrol at c(4) and c(5) of the newly formed tetrahydrofuran rings is observed. use of a chiral auxiliary allows the preparation of enantiomerically pure material. the utility of this method has been demonstrated by achieving a short synthesis of (+)-eldanolide, the pheromone of the ...200010804554
constituents of wing gland and abdominal hair pencil secretions of male african sugarcane borer,eldana saccharina walker (lepidoptera: pyralidae).in addition totrans-3,7-dimethyl-6-octen-4-olide (eldanolide), vanillin, and 4-hydroxybenzaldehyde, identified by french workers in the wing gland and abdominal hair pencil secretions of the male african sugarcane borer,eldana saccharina, we have, in an earlier note, reported the presence of several other terpenoid, aromatic, and unbranched-chain compounds such as, (z)-3,7-dimethylocta-2,6-dienoic acid, 6,10,14-trimethyl-2-pentadecanol, 4-hydroxy-3-methoxybenzyl alcohol, 1-octadecane thiol, 16-h ...199324248574
predicting hotspots of human-elephant conflict to inform mitigation strategies in xishuangbanna, southwest china.research on the spatial patterns of human-wildlife conflict is fundamental to understanding the mechanisms underlying it and to identifying opportunities for mitigation. in the state of xishuangbanna, containing china's largest tropical forest, an imbalance between nature conservation and economic development has led to increasing conflicts between humans and asian elephants (elephas maximus), as both elephant numbers and conversion of habitable land to rubber plantations have increased over the ...201627631976
relative toxicity of two aphicides to hippodamia convergens (coleoptera: coccinellidae): implications for integrated management of sugarcane aphid, melanaphis sacchari (hemiptera: aphididae).flupyradifurone and sulfoxaflor present novel insecticide chemistries with particular efficacy against aphids, and the recent emergence of sugarcane aphid, melanaphis sacchari (zehntner), as a pest of sorghum in the united states has resulted in their widespread use. we examined their toxicity to hippodamia convergens guerin-meneville, an important aphid biocontrol agent. we exposed beetles to topical applications of the field rate (fr) of these insecticides, fed them contaminated food (eggs of ...201728039423
indigenous aphid predators show high levels of preadaptation to a novel prey, melanaphis sacchari (hemiptera: aphididae).the performance of four aphid predators, hippodamia convergens guerin-meneville, coleomegilla maculata degeer, chrysoperla carnea stephens and orius insidiosus say was compared on three prey species: schizaphis graminum rondani, melanaphis sacchari (zehntner), and ephestia kuehniella zeller eggs. species predatory in both life stages (all except ch. carnea) were reared on e. kuehniella eggs and switched to aphid prey for assessment of reproduction. differences were greater between the e. kuehnie ...201526470381
in situ enzyme aided adsorption of soluble xylan biopolymers onto cellulosic material.the functional properties of cellulose fibers can be modified by adsorption of xylan biopolymers. the adsorption is improved when the degree of biopolymers substitution with arabinose and 4-o-methyl-glucuronic acid (meglca) side groups, is reduced. α-l-arabinofuranosidase (abfb) and α-d-glucuronidase (agua) enzymes were applied for side group removal, to increase adsorption of xylan from sugarcane (saccharum officinarum l) bagasse (bh), bamboo (bambusa balcooa) (bm), pinus patula (pp) and eucaly ...201627083357
modifying solubility of polymeric xylan extracted from eucalyptus grandis and sugarcane bagasse by suitable side chain removing enzymes.α-l-arabinofuranosidase (abfb) and novel α-d-glucuronidase (agu1b) enzymes were applied for selective hydrolysis of beechwood (fagus sylvatica) xylan (sigma-aldrich) as well as xylans extracted from eucalyptus grandis and sugarcane (saccharum officinarum l.) bagasse, leading to precipitation of these carbohydrate biopolymers. hemicellulose extraction was performed with two mild-alkali methods, höije and pinto. precipitation occurred after removal of 67, 40 and 16% 4-o-methyl-d-glucuronic acid (m ...201526256174
consistent patterns in leaf lamina and leaf vein carbon isotope composition across ten herbs and tree species.wide-spread post-photosynthetic fractionation processes deplete metabolites and plant compartments in (13)c relative to assimilates to varying degrees. fragmentation fractionation and exchange of metabolites with distinct isotopic signatures across organ boundaries further modify the patterns of plant isotopic composition. heterotrophic organs tend to become isotopically heavier than the putative source material as a result of respiratory metabolism. in addition fractionation may occur during me ...200919603469
phytotherapy in medieval serbian medicine according to the pharmacological manuscripts of the chilandar medical codex (15-16th centuries).the chilandar medical codex is the most significant and best preserved medieval serbian manuscript and collects together documents on european medical science from the 12th to 15th centuries. it represents the best-known and most complete example of a large collection of medical manuscripts from the salerno-montpellier school, written in the vernacular - something which does not exist among the majority of european nations. this paper presents the section of the codex that deals with phytotherap ...201121708242
ecotoxicological characterization of sugarcane vinasses when applied to tropical soils.the impact of sugarcane vinasse on soil invertebrates was assessed through ecotoxicological assays. increasing concentrations of two vinasses from different distillery plants (va and vb), and a vinasse from a laboratory production (vc), were amended on two natural tropical oxisols (lv and lva) and a tropical artificial soil (tas) to characterize the effects of the vinasses on earthworms (eisenia andrei), enchytraeids (enchytraeus crypticus), mites (hypoaspis aculeifer) and collembolans (folsomia ...201525933292
integrated ecological risk assessment of pesticides in tropical ecosystems: a case study with carbofuran in brazil.the aim of the present study is to contribute an ecologically relevant assessment of the ecotoxicological effects of pesticide applications in agricultural areas in the tropics, using an integrated approach with information gathered from soil and aquatic compartments. carbofuran, an insecticide/nematicide used widely on sugarcane crops, was selected as a model substance. to evaluate the toxic effects of pesticide spraying for soil biota, as well as the potential indirect effects on aquatic biota ...201222068639
effects of gna transgenic sugarcane on life history parameters of parallorhogas pyralophagus (marsh) (hymenoptera: braconidae), a parasitoid of mexican rice borer.parallorhogas pyralophagus (marsh) is the principal parasitoid of the mexican rice borer [eoreuma loftini (dyar)], the primary pest of sugarcane in south texas. insect resistant transgenic sugarcane producing galanthus nivalis agglutinin (gna) was developed to improve control of e. loftini. the present laboratory study addressed whether over two consecutive generations transgenic sugarcane delivered via artificial diet has adverse host-mediated effects on p. pyralophagus life history parameters. ...200312852590
biochemical compositions and fatty acid profiles in four species of microalgae cultivated on household sewage and agro-industrial residues.the potential of four regional microalgae species was evaluated in relation to their cell growth and biomass production when cultured in the following alternative media: bio-composts of fruit/horticultural wastes (hb), sugarcane waste and vinasse (vb) chicken excrements (bce), raw chicken manure (rcm), and municipal domestic sewage (mds). the cultures were maintained under controlled conditions and their growth responses, productivities, biochemical compositions, and the ester profiles of their ...201627668876
effects of two different organic amendments addition to soil on sorption-desorption, leaching, bioavailability of penconazole and the growth of wheat (triticum aestivum l.).this study investigated the effects of sugarcane bagasse compost (sbc) and chicken manure compost (cmc) on the sorption-desorption, leaching and bioavailability of the fungicide penconazole in soil in a laboratory setting. the autoclave-treated sbc or cmc was applied at 2.5% and 5.0% (w/w). results of batch equilibrium experiments exhibited that the sorption capacity of soils for penconazole was significantly promoted by the addition of sbc or cmc, whereas desorption of penconazole was drastical ...201626683765
estimating 20-year land use change and derived co2 emissions associated to crops, pasture and forestry in brazil and each of its 27 states.land use change (luc) in brazil has important implications on global climate change, ecosystem services and biodiversity, and agricultural expansion plays a critical role in this process. concerns over these issues have led to the need for estimating the magnitude and impacts associated to that, which are increasingly reported in the environmental assessment of products. currently, there is an extensive debate on which methods are more appropriate for estimating luc and related emissions and reg ...201728370797
techno-economic analysis and climate change impacts of sugarcane biorefineries considering different time horizons.ethanol production from lignocellulosic feedstocks (also known as 2nd generation or 2g ethanol process) presents a great potential for reducing both ethanol production costs and climate change impacts since agricultural residues and dedicated energy crops are used as feedstock. this study aimed at the quantification of the economic and environmental impacts considering the current and future scenarios of sugarcane biorefineries taking into account not only the improvements of the industrial proc ...201728293288
effects of air pollution caused by sugarcane burning in western são paulo on the cardiovascular system.to evaluate the effects of acute exposure to air pollutants (no2 and pm10) on hospitalization of adults and older people with cardiovascular diseases in western são paulo.201728273230
defense-related proteins involved in sugarcane responses to biotic stress.sugarcane is one of the most important agricultural crops in the world. however, pathogen infection and herbivore attack cause constant losses in yield. plants respond to pathogen infection by inducing the expression of several protein types, such as glucanases, chitinases, thaumatins, peptidase inhibitors, defensins, catalases and glycoproteins. proteins induced by pathogenesis are directly or indirectly involved in plant defense, leading to pathogen death or inducing other plant defense respon ...201728222203
unraveling the influence of the cod/sulfate ratio on organic matter removal and methane production from the biodigestion of sugarcane vinasse.throughout the sugarcane harvest, it is common for sulfate to accumulate in the vinasse of sugar and ethanol plants. however, little is known regarding the influence of sulfate on the anaerobic digestion (ad) of vinasse, which may lead to severe performance losses. this study assessed the influence of various cod/sulfate ratios (12.0, 10.0 and 7.5) on both cod removal and methane (ch4) production from sugarcane vinasse ad. batch assays were conducted in thermophilic conditions. at a cod/sulfate ...201728214696
biochemical composition of symplastic sap from sugarcane genetically modified to overproduce proline.global interest in sugarcane has increased significantly in recent years because of its economic impact on sustainable energy production. the purpose of the present study was to evaluate changes in the concentrations of total sugars, amino acids, free proline, and total proteins by colorimetric analyses and nuclear magnetic resonance (nmr) to perform a metabolic profiling of a water-soluble fraction of symplastic sap in response to the constitutive expression of a mutant δ(1)-pyrroline-5-carboxy ...201728213180
effect of biogas sparging on the performance of bio-hydrogen reactor over a long-term operation.this study aimed to enhance hydrogen production from sugarcane syrup by biogas sparging. two-stage continuous stirred tank reactor (cstr) and upflow anaerobic sludge blanket (uasb) reactor were used to produce hydrogen and methane, respectively. biogas produced from the uasb was used to sparge into the cstr. results indicated that sparging with biogas increased the hydrogen production rate (hpr) by 35% (from 17.1 to 23.1 l/l.d) resulted from a reduction in the hydrogen partial pressure. a fluctu ...201728207755
development of a low-cost cellulase production process using trichoderma reesei for brazilian biorefineries.during the past few years, the first industrial-scale cellulosic ethanol plants have been inaugurated. although the performance of the commercial cellulase enzymes used in this process has greatly improved over the past decade, cellulases still represent a very significant operational cost. depending on the region, transport of cellulases from a central production facility to a biorefinery may significantly add to enzyme cost. the aim of the present study was to develop a simple, cost-efficient ...201728184245
d-mannitol sensor based on molecularly imprinted polymer on electrode modified with reduced graphene oxide decorated with gold nanoparticles.an electrochemical sensor for d-mannitol based on molecularly imprinted polymer on electrode modified with reduced graphene oxide decorated with gold nanoparticles was developed in this present work. the sensor was constructed for the first time via the electropolymerization of o-phenylenediamine (o-pd) over a surface containing reduced graphene oxide (rgo) and gold nanoparticles (aunp) in the presence of d-mannitol molecules. the surface modification with aunp/rgo-gce facilitated the charge tra ...201728153247
comet assay and micronucleus tests on oreochromis niloticus (perciforme: cichlidae) exposed to raw sugarcane vinasse and to phisicochemical treated vinasse by ph adjustment with lime (cao).in brazil vinasse, a main sugarcane distillery residue, stands out because every liter of alcohol generates 10-15 l of vinasse as waste. an alternative for the disposal of this waste is the fertirrigation of the sugarcane culture itself. however, the high amount released can saturate the soil and through leaching/percolation contaminate water resources. the aim of this study is verifying the toxic potential of vinasse in tilapias and effectiveness of the physicalchemical treatment of this waste ...201728131919
analysis of three sugarcane homo/homeologous regions suggests independent polyploidization events of saccharum officinarum and saccharum spontaneum.whole genome duplication has played an important role in plant evolution and diversification. sugarcane is an important crop with a complex hybrid polyploid genome, for which the process of adaptation to polyploidy is still poorly understood. in order to improve our knowledge about sugarcane genome evolution and the homo/homeologous gene expression balance, we sequenced and analyzed 27 bacs (bacterial artificial chromosome) of sugarcane r570 cultivar, containing the putative single-copy genes lf ...201728082603
a kinetic model for hydrothermal pretreatment of sugarcane straw.this work presents kinetic models of cellulose and hemicellulose extraction during hydrothermal pretreatment of sugarcane straw. biomass was treated under conditions of 180, 195, and 210°c, using a solid/liquid ratio of 1:10 (w/v). in this study, cellobiose, glucose, formic acid and hydroxymethylfurfural (from cellulosic fraction) and xylose, arabinose, acetic acid, glucuronic acid and furfural (from hemicellulosic fraction) were taken into account in the kinetic parameters determination. the gl ...201728063360
microalgae cultivation in sugarcane vinasse: selection, growth and biochemical characterization.sugarcane ethanol is produced at large scale generating wastes that could be used for microalgae biomass production in a biorefinery strategy. in this study, forty microalgae strains were screened for growth in sugarcane vinasse at different concentrations. two microalgae strains, micractinium sp. embrapa|lba32 and c. biconvexa embrapa|lba40, presented vigorous growth in a light-dependent manner even in undiluted vinasse under non-axenic conditions. microalgae strains presented higher biomass pr ...201728061395
impacts of converting low-intensity pastureland to high-intensity bioenergy cropland on the water quality of tropical streams in brazil.in brazil, the cultivation of bioenergy crops is expanding at an accelerated rate. most of this expansion has occurred over low-intensity pasture and is considered sustainable because it does not involve deforestation of natural vegetation. however, the impacts on the water quality of headwater streams are poorly understood, especially with regard to the influence of land use patterns in the watershed. in this study, we investigated the effects of land-use conversion on the water quality of stre ...201728040217
a lignocellulosic hydrolysate-tolerant aurantiochytrium sp. mutant strain for docosahexaenoic acid production.to utilize lignocellulosic hydrolysate for docosahexaenoic acid (dha) production, a novel mutant aurantiochytrium sp. fn21 with strong tolerance against inhibitory lignocellulosic hydrolysate was obtained through continuous domestication processes from the parent strain aurantiochytrium sp. fju-512. aurantiochytrium sp. fn21 can accumulate 21.3% and 30.7% more dha compared to its parent strain cultured in fermentation medium and a medium with 50% (v/v) sugarcane bagasse hydrolysate (sbh), respec ...201728038399
in planta production and characterization of a hyperthermostable gh10 xylanase in transgenic sugarcane.sugarcane (saccharum sp. hybrids) is one of the most efficient and sustainable feedstocks for commercial production of fuel ethanol. recent efforts focus on the integration of first and second generation bioethanol conversion technologies for sugarcane to increase biofuel yields. this integrated process will utilize both the cell wall bound sugars of the abundant lignocellulosic sugarcane residues in addition to the sucrose from stem internodes. enzymatic hydrolysis of lignocellulosic biomass in ...201728005227
functional analysis of oxidative burst in sugarcane smut-resistant and -susceptible genotypes.smut pathogen induced an early modulation of the production and scavenging of reactive oxygen species during defence responses in resistant sugarcane that coincided with the developmental stages of fungal growth. sporisorium scitamineum is the causal agent of sugarcane smut disease. in this study, we characterized sugarcane reactive oxygen species (ros) metabolism in response to the pathogen in smut-resistant and -susceptible genotypes. sporisorium scitamineum teliospore germination and appresso ...201728004180
detection of bacillus and stenotrophomonas species growing in an organic acid and endocrine-disrupting chemical-rich environment of distillery spent wash and its phytotoxicity.sugarcane molasses-based distillery spent wash (dsw) is well known for its toxicity and complex mixture of various recalcitrant organic pollutants with acidic ph, but the chemical nature of these pollutants is unknown. this study revealed the presence of toxic organic acids (butanedioic acid bis(tms)ester; 2-hydroxysocaproic acid; benzenepropanoic acid, α-[(tms)oxy], tms ester; vanillylpropionic acid, bis(tms)), and other recalcitrant organic pollutants (2-furancarboxylic acid, 5-[[(tms)oxy] met ...201728000121
continuous hydrogen production from agricultural wastewaters at thermophilic and hyperthermophilic temperatures.the objective of this study was to investigate the effects of hydraulic retention time (hrt) (8 to 0.5 h) and temperature (55 to 75 °c) in two anaerobic fluidized bed reactors (afbr) using cheese whey (afbr-cw = 10,000 mg sugars l(-1)) and vinasse (afbr-v = 10,000 mg cod l(-1)) as substrates. decreasing the hrt to 0.5 h increased the hydrogen production rates in both reactors, with maximum values of 5.36 ± 0.81 l h2 h(-1) l(-1) in afbr-cw and 0.71 ± 0.16 l h2 h(-1) l(-1) in afbr-v. the optimal c ...201627987191
pilot scale biodiesel production from microbial oil of rhodosporidium toruloides debb 5533 using sugarcane juice: performance in diesel engine and preliminary economic study.a successful pilot-scale process for biodiesel production from microbial oil (biooil) produced by rhodosporidium toruloides debb 5533 is presented. using fed-batch strategy (1000l working volume), a lipid productivity of 0.44g/l.h was obtained using a low-cost medium composed by sugarcane juice and urea. the microbial oil was used for biodiesel production and its performance was evaluated in diesel engine tests, showing very good performance, especially for the blend b20 sco, when operating at 2 ...201627969577
production of biodegradable plastic by polyhydroxybutyrate (phb) accumulating bacteria using low cost agricultural waste material.polyhydroxybutyrates (phbs) are macromolecules synthesized by bacteria. they are inclusion bodies accumulated as reserve materials when the bacteria grow under different stress conditions. because of their fast degradability under natural environmental conditions, phbs are selected as alternatives for production of biodegradable plastics. the aim of this work was to isolate potential phb producing bacteria, evaluate phb production using agro-residues as carbon sources.201627955705
nutrient availability shapes the microbial community structure in sugarcane bagasse compost-derived consortia.microbial communities (mcs) create complex metabolic networks in natural habitats and respond to environmental changes by shifts in the community structure. although members of mcs are often not amenable for cultivation in pure culture, it is possible to obtain a greater diversity of species in the laboratory setting when microorganisms are grown as mixed cultures. in order to mimic the environmental conditions, an appropriate growth medium must be applied. here, we examined the hypothesis that ...201627941835
increased sink strength offsets the inhibitory effect of sucrose on sugarcane photosynthesis.spraying sucrose inhibits photosynthesis by impairing rubisco activity and stomatal conductance (gs), whereas increasing sink demand by partially darkening the plant stimulates sugarcane photosynthesis. we hypothesized that the stimulatory effect of darkness can offset the inhibitory effect of exogenous sucrose on photosynthesis. source-sink relationship was perturbed in two sugarcane cultivars by imposing partial darkness, spraying a sucrose solution (50mm) and their combination. five days afte ...201727889522
biological pretreatment of sugarcane bagasse with basidiomycetes producing varied patterns of biodegradation.this work evaluated sugarcane bagasse pretreatment with wood-decay fungi, producing varied patterns of biodegradation. the overall mass balance of sugars released after pretreatment and enzymatic hydrolysis indicated that a selective white-rot was necessary to provide glucose yields similar to the ones observed from leading physico-chemical pretreatment technologies. the selective white-rot ceriporiopsis subvermispora was selective for lignin degradation in the lignocellulosic material, preserve ...201727875764
transgenic sugarcane overexpressing canecpi-1 negatively affects the growth and development of the sugarcane weevil sphenophorus levis.transgenic sugarcane expressing canecpi-1 exhibits resistance to sphenophorus levis larvae. transgenic plants have widely been used to improve resistance against insect attack. sugarcane is an economically important crop; however, great losses are caused by insect attack. sphenophorus levis is a sugarcane weevil that digs tunnels in the stem base, leading to the destruction of the crop. this insect is controlled inefficiently by chemical insecticides. transgenic plants expressing peptidase inhib ...201727837261
landscape, environmental and social predictors of hantavirus risk in são paulo, brazil.hantavirus pulmonary syndrome (hps) is a disease caused by hantavirus, which are negative-sense rna viruses in the family bunyaviridae that are highly virulent to humans. numerous factors modify risk of hantavirus transmission and consequent hps risk. human-driven landscape change can foster transmission risk by increasing numbers of habitat generalist rodent species that serve as the principal reservoir host. climate can also affect rodent population dynamics and hantavirus survival, and a numb ...201627780250
histopathological analysis of tilapia gills (oreochromis niloticus linnaeus, 1758) exposed to sugarcane vinasse.sugarcane vinasse is one of the main residues generated by the transformation of cane into ethanol. because of the high organic content (cod), high biochemical oxygen demand (bod), low ph, the large amount that this residue is generated (15l for every liter of ethanol produced) and their use as fertilizer on the sugarcane crop, this residue is potentially polluting to the soil ecossystem and by percolation to water ecossystem too. thus, this study aimed to assess the toxicity of vinasse by analy ...201727770647
comprehensive analysis of the cellulolytic system reveals its potential for deconstruction of lignocellulosic biomass in a novel streptomyces sp.the giant snail achatina fulica is considered an invasive species in most territories in which it was introduced, due to its ability to process a large amount of lignocellulose as a consequence of the presence of a cellulolytic-associated microflora. streptomyces are well known as crucial agents in the decomposition of complex polymers in soil environments and also as cellulolytic symbionts commonly associated with herbivore insects. here, we employed a combination of genomic and biochemical too ...201727699477
optimization of substrate preparation for oyster mushroom (pleurotus ostreatus) cultivation by studying different raw materials and substrate preparation conditions (composting: phases i and ii).in recent years, oyster mushroom (pleurotus ostreatus) has become one of the most cultivated mushrooms in the world, mainly in brazil. among many factors involved in a mushroom production, substrate preparation is the most critical step, which can be influenced by composting management techniques. looking forward to optimizing the substrate preparation process, were tested different composting conditions (7 and 14 days of composting with or without conditioning), potential raw materials (decumbe ...201627696289
phosphate solubilization potential of rhizosphere fungi isolated from plants in jimma zone, southwest ethiopia.phosphorus (p) is one of the major bioelements limiting agricultural production. phosphate solubilizing fungi play a noteworthy role in increasing the bioavailability of soil phosphates for plants. the present study was aimed at isolating and characterizing phosphate solubilizing fungi from different rhizospheres using both solid and liquid pikovskaya (pvk) medium. a total of 359 fungal isolates were obtained from 150 rhizosphere soil samples of haricot bean, faba bean, cabbage, tomato, and suga ...201627688771
soybean protein as a cost-effective lignin-blocking additive for the saccharification of sugarcane bagasse.addition of surfactants, polymers, and non-catalytic proteins can improve the enzymatic hydrolysis of lignocellulosic materials by blocking the exposed lignin surfaces, but involves extra expense. here, soybean protein, one of the cheapest proteins available, was evaluated as an alternative additive for the enzymatic hydrolysis of pretreated sugarcane bagasse. the effect of the enzyme source was investigated using enzymatic cocktails from a. niger and t. reesei cultivated under solid-state, subm ...201627639236
liming in the sugarcane burnt system and the green harvest practice affect soil bacterial community in northeastern são paulo, brazil.here we show that both liming the burnt sugarcane and the green harvest practice alter bacterial community structure, diversity and composition in sugarcane fields in northeastern são paulo state, brazil. terminal restriction fragment length polymorphism fingerprinting and 16s rrna gene cloning and sequencing were used to analyze changes in soil bacterial communities. the field experiment consisted of sugarcane-cultivated soils under different regimes: green sugarcane (gs), burnt sugarcane (bs), ...201627629424
phenolic compounds and anticancer activity of commercial sugarcane cultivated in brazil.the cultivation of sugarcane hybrids (x saccharum officinarum l.) is an important revenue source for the brazilian economy. herein it is reported the evaluation of the cytotoxic activity of mid-polarity sugarcane extracts against human cancer cell lines, as well as the isolation of steroids sitosterol, stigmasterol and campesterol, phenolic acids p-hydroxybenzoic, p-hydroxycinnamic, vanillic and ferulic acid, terpenoids α-tocopherol and β-carotene and a novel substance in sugarcane, the flavonoi ...201627598841
synthesis of cellulose acetate and carboxymethylcellulose from sugarcane straw.sugarcane straw (scs) is a raw material with high potential for production of cellulose derivatives due to its morphology and structure. the proposal of this work was to synthesize cellulose acetate (ca) and carboxymethylcellulose (cmc) from sugarcane straw cellulose, and applied the ca in the preparation of a membrane. the cellulose extraction was carried out in four steps. firstly, scs was treated with h2so4 (10% v/v) followed by naoh (5% w/v) treatment. subsequently, a chelating process was p ...201627516319
effects of genotype, wilting, and additives on the nutritive value and fermentation of bermudagrass silage.bermudagrass is the main warm-season grass species used for livestock production in the southeastern united states; however, when it is ensiled, the silage fermentation parameters are often less than desirable. the objective of this study was to investigate the effects of management practices on the nutritive value and fermentation characteristics of bermudagrass silage. in exp. 1, treatments were the factorial combinations of 2 bermudagrass genotypes, 'jiggs' () and 'tifton 85' ( sp.), 4 additi ...201627482693
nitrous oxide emission related to ammonia-oxidizing bacteria and mitigation options from n fertilization in a tropical soil.nitrous oxide (n2o) from nitrogen fertilizers applied to sugarcane has high environmental impact on ethanol production. this study aimed to determine the main microbial processes responsible for the n2o emissions from soil fertilized with different n sources, to identify options to mitigate n2o emissions, and to determine the impacts of the n sources on the soil microbiome. in a field experiment, nitrogen was applied as calcium nitrate, urea, urea with dicyandiamide or 3,4 dimethylpyrazone phosp ...201627460335
characterization of sugarcane (saccharum spp.) leaf senescence: implications for biofuel production.second-generation ethanol (2g-bioethanol) uses lignocellulosic feedstocks for ethanol production. sugarcane is one among the most suitable crops for biofuel production. its juice is extracted for sugar production, while sugarcane bagasse, straw, and senescing leaves are considered industrial waste. senescence is the age-dependent deterioration of plant cells, ultimately leading to cell death and completion of the plant life cycle. because senescing leaves may also be used for biofuel production, ...201627453728
a new genus and two new species of soldier fly (diptera: stratiomyidae: chiromyzinae) from australia, one found infesting sugarcane in central queensland.metridius nov. gen. and types species m. robertsoni nov. sp. with winged males and apterous females is described from adults and larvae found infesting sugarcane stools from near mackay, central queensland. a second new species, m. mcalpinei nov. sp., based only on males from near sydney, new south wales is also described. notes on the biology of both species and an identification key to the genera of the subfamily chiromyzinae and to the species are also given.201627394475
unlocking the bacterial and fungal communities assemblages of sugarcane microbiome.plant microbiome and its manipulation herald a new era for plant biotechnology with the potential to benefit sustainable crop production. however, studies evaluating the diversity, structure and impact of the microbiota in economic important crops are still rare. here we describe a comprehensive inventory of the structure and assemblage of the bacterial and fungal communities associated with sugarcane. our analysis identified 23,811 bacterial otus and an unexpected 11,727 fungal otus inhabiting ...201627358031
treatment of sugarcane vinasse by combination of coagulation/flocculation and fenton's oxidation.the efficiency of individual and integrated processes applied to organic matter reduction and biodegradability improvement of a biodigested sugarcane vinasse wastewater was assessed. strategies considered were fenton's oxidation (strategy 1), coagulation/flocculation (strategy 2) and the combination of both processes (coagulation/flocculation followed by fenton's reaction) - strategy 3. it was found that fenton's oxidation per se allowed reducing the organic matter, increasing the wastewater bio ...201627353374
genome-wide association mapping of quantitative traits in a breeding population of sugarcane.molecular markers associated with relevant agronomic traits could significantly reduce the time and cost involved in developing new sugarcane varieties. previous sugarcane genome-wide association analyses (gwas) have found few molecular markers associated with relevant traits at plant-cane stage. the aim of this study was to establish an appropriate gwas to find molecular markers associated with yield related traits consistent across harvesting seasons in a breeding population. sugarcane clones ...201627342657
effect of ph, temperature, and chemicals on the endoglucanases and β-glucosidases from the thermophilic fungus myceliophthora heterothallica f.2.1.4. obtained by solid-state and submerged cultivation.this work reports endoglucanase and beta-glucosidase production by the thermophilic fungus myceliophthora heterothallica in solid-state (ssc) and submerged (smc) cultivation. wheat bran and sugarcane bagasse were used for ssc and cardboard for smc. highest endoglucanase production in ssc occurred after 192 hours: 1,170.6 ± 0.8 u/g, and in smc after 168 hours: 2,642 ± 561 u/g. the endoglucanases and beta-glucosidases produced by both cultivation systems showed slight differences concerning their ...201627242927
gh10 xyna is the main xylanase identified in the crude enzymatic extract of paenibacillus sp. a59 when grown on xylan or lignocellulosic biomass.a novel bacterial isolate with polysaccharides degrading activity was identified as paenibacillus sp., and named paenibacillus sp. a59. even though it is a strict mesophile, optimal xylanase activity of the crude enzymatic extract was achieved between 50°c and 70°c and more than 60% of the activity was retained after incubation for 48h at 50°c, indicating thermotolerance of the enzymes involved. the extract was also active on pre-treated sugarcane residue (scr) and wheat straw, releasing xylobio ...201727242139
exploring glycoside hydrolases and accessory proteins from wood decay fungi to enhance sugarcane bagasse saccharification.glycoside hydrolases (ghs) and accessory proteins are key components for efficient and cost-effective enzymatic hydrolysis of polysaccharides in modern, biochemically based biorefineries. currently, commercialized ghs and accessory proteins are produced by ascomycetes. however, the role of wood decay basidiomycetes proteins in biomass saccharification has not been extensively pursued. wood decay fungi degrade polysaccharides in highly lignified tissues in natural environments, and are a promisin ...201627222665
determination of pesticides in sugarcane juice employing microextraction by packed sorbent followed by gas chromatography and mass spectrometry.this paper describes the development of a method for the determination of six pesticides (tebuthiuron, carbofuran, atrazine, metribuzine, ametryn, and bifenthrin) in sugarcane juice using microextraction by packed sorbent as the extraction technique. the extraction steps were optimized by factorial design, being the variables ph, ionic strength, desorption solvent and solvent volume optimized for comparisons among sorbent materials. among the evaluated materials c18-chromabond(®) showed better e ...201627219489
air pollutants and hospital admission due to pneumonia in children: a time series analysis.the aim of this study was to estimate the association between expo sure to air pollutants and hospitalization for pneumonia among children in a medium-sized city located in the sugar cane plantation region of são paulo state.201627167545
tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance.grasses are lignocellulosic materials useful to supply the billion-tons annual requirement for renewable resources that aim to produce transportation fuels and a variety of chemicals. however, the polysaccharides contained in grass cell walls are built in a recalcitrant composite. deconstruction of these cell walls is still a challenge for the energy-efficient and economically viable transformation of lignocellulosic materials. the varied tissue-specific distribution of cell wall components adds ...201627148403
loss of soil (macro)fauna due to the expansion of brazilian sugarcane acreage.land use changes (luc) from pasture to sugarcane (saccharum spp.) crop are expected to add 6.4mha of new sugarcane land by 2021 in the brazilian cerrado and atlantic forest biomes. we assessed the effects of these luc on the abundance and community structure of animals that inhabit soils belowground through a field survey using chronosequences of land uses comprising native vegetation, pasture, and sugarcane along a 1000-km-long transect across these two major tropical biomes in brazil. macrofau ...201627135579
boosting tag accumulation with improved biodiesel production from novel oleaginous microalgae scenedesmus sp. iitrind2 utilizing waste sugarcane bagasse aqueous extract (sbae).this investigation utilized sugarcane bagasse aqueous extract (sbae), a nontoxic, cost-effective medium to boost triacylglycerol (tag) accumulation in novel fresh water microalgal isolate scenedesmus sp. iitrind2. maximum lipid productivity of 112 ± 5.2 mg/l/day was recorded in microalgae grown in sbae compared to modified bbm (26 ± 3 %). carotenoid to chlorophyll ratio was 12.5 ± 2 % higher than in photoautotrophic control, indicating an increase in photosystem ii activity, thereby increasing g ...201627093970
raoultella sp. strain l03 fixes n2 in association with micropropagated sugarcane plants.n2 -fixing bacteria belonging to the genus raoultella of the family enterobacteriaceae are widely associated with plants. raoultella sp. strain l03 was isolated from surface-sterilized sugarcane roots. in this study, we inoculated the strain l03 to microbe-free micropropagated plantlets of the main sugarcane cultivar roc22 grown in guangxi, china and determined n2 -fixation and association between strain l03 and sugarcane plants. inoculation of strain l03 increased plant biomass, total n, n conc ...201627059698
thermal decomposition of sugarcane straw, kinetics and heat of reaction in synthetic air.the aim of this work was to analyze the thermal decomposition, kinetics and heat of reaction of sugarcane straw in synthetic air by thermogravimetry (tg) and differential scanning calorimetry (dsc). the tg and dsc experiments were carried out using heating rates of 2.5°c/min, 5°c/min, and 10°c/min, and particle diameter of 0.250mm. in the study of the smoldering reaction were identified three consecutive stages, drying, oxidative pyrolysis, and combustion. thus, the kinetic pathway was composed ...201627019126
exogenous nitric oxide improves sugarcane growth and photosynthesis under water deficit.nitric oxide (no)-mediated redox signaling plays a role in alleviating the negative impact of water stress in sugarcane plants by improving root growth and photosynthesis. drought is an environmental limitation affecting sugarcane growth and yield. the redox-active molecule nitric oxide (no) is known to modulate plant responses to stressful conditions. no may react with glutathione (gsh) to form s-nitrosoglutathione (gsno), which is considered the main reservoir of no in cells. here, we investig ...201627002974
cobalt internal standard for ni to assist the simultaneous determination of mo and ni in plant materials by high-resolution continuum source graphite furnace atomic absorption spectrometry employing direct solid sample analysis.a new method is proposed for the simultaneous determination of mo and ni in plant materials by high-resolution continuum source graphite furnace atomic absorption spectrometry (hr-cs gfaas), employing direct solid sample analysis (dss) and internal standardization (is). cobalt was used as internal standard to minimize matrix effects during ni determinations, enabling the use of aqueous standards for calibration. correlation coefficients for the calibration curves were typically better than 0.993 ...201626992542
production of cellulosic ethanol from sugarcane bagasse by steam explosion: effect of extractives content, acid catalysis and different fermentation technologies.the production of cellulosic ethanol was carried out using samples of native (ncb) and ethanol-extracted (eecb) sugarcane bagasse. autohydrolysis (ah) exhibited the best glucose recovery from both samples, compared to the use of both h3po4 and h2so4 catalysis at the same pretreatment time and temperature. all water-insoluble steam-exploded materials (seb-wi) resulted in high glucose yields by enzymatic hydrolysis. shf (separate hydrolysis and fermentation) gave ethanol yields higher than those o ...201626943936
estimating the opportunity costs of environmental conservation in the feijão river watershed (são carlos-sp, brazil).the objective of this study was to infer the opportunity cost of land use of the feijão river watershed (são carlos-sp, brazil), in order to estimate the financial resources necessary to compensate landowners willing to convert their production areas into areas of environmental preservation. net values were estimated by calculating the annual value and the net present value of each activity. the area used for agricultural production was estimated using the land cover map of the watershed. the st ...201626909620
concentration of cu, zn, cr, ni, cd, and pb in soil, sugarcane leaf and juice: residual effect of sewage sludge and organic compost application.many researchers have evaluated the effects of successive applications of sewage sludge (ss) on soil plant-systems, but most have not taken into account the residual effect of organic matter remaining from prior applications. furthermore, few studies have been carried out to compare the effects of the agricultural use of ss and sewage sludge compost (ssc). therefore, we evaluated the residual effect of ss and ssc on the heavy metal concentrations in soil and in sugarcane (saccharum spp.) leaves ...201626879984
genome organization and host range of a brazilian isolate of johnsongrass mosaic virus.this work reports the complete genome sequence, production of a polyclonal antiserum, and host range of a brazilian strain of johnsongrass mosaic virus (jgmv) found infecting panicum maximum in the state of são paulo, brazil. the complete genome sequence of this potyvirus, comprising 9874 nucleotides, showed 82 % amino acid sequence identity in the polyprotein to that of an isolate of jgmv from australia. the experimental host range of this virus included mainly fodder species. cultivated specie ...201626869279
estimating the opportunity costs of environmental conservation in the feijão river watershed (são carlos-sp, brazil).the objective of this study was to infer the opportunity cost of land use of the feijão river watershed (são carlos-sp, brazil), in order to estimate the financial resources necessary to compensate landowners willing to convert their production areas into areas of environmental preservation. net values were estimated by calculating the annual value and the net present value of each activity. the area used for agricultural production was estimated using the land cover map of the watershed. the st ...201626840574
molecular characterization of soil organic matter from native vegetation-pasture-sugarcane transitions in brazil.replacing pastures (pa) with sugarcane (sg) has been deemed an agronomically feasible strategy for sugarcane expansion in brazil. however, there are some uncertainties about the environmental impacts regarding this land use change (luc), mainly related to soil organic matter (som), a key factor of environmental sustainability of brazilian ethanol. luc-related losses of som can overcome the c savings from biofuels. the molecular composition of som was evaluated to understand the c dynamics regard ...201626828621
cell wall proteome of sugarcane stems: comparison of a destructive and a non-destructive extraction method showed differences in glycoside hydrolases and peroxidases.sugarcane has been used as the main crop for ethanol production for more than 40 years in brazil. recently, the production of bioethanol from bagasse and straw, also called second generation (2g) ethanol, became a reality with the first commercial plants started in the usa and brazil. however, the industrial processes still need to be improved to generate a low cost fuel. one possibility is the remodeling of cell walls, by means of genetic improvement or transgenesis, in order to make the bagass ...201626754199
monitoring of the cellulosic ethanol fermentation process by near-infrared spectroscopy.rapid, efficient, and low-cost technologies for monitoring the fermentation process during second generation (2g) or cellulosic ethanol production are essential for the successful implementation of this process at the commercial scale. here, the use of near-infrared (nir) spectroscopy associated with partial least squares (pls) regression was investigated as a tool for monitoring the production of 2g ethanol from lignocellulosic sugarcane residues including bagasse, straw, and tops. the spectral ...201626748047
comparison of submerged and solid state pretreatment of sugarcane bagasse by pandoraea sp. istkb: enzymatic and structural analysis.pretreatment of sugarcane bagasse by pandoraea sp. istkb was evaluated under submerged (smf) and solid state (ssf) culture conditions. number of bacteria was 2.7 times higher in smf as compared with ssf. enzymes produced under smf were xylanase, lip, mnp and laccase. in ssf xylanase and laccase were detected. cmcase, fpase and β-glucosidase were not detected. delignification was highest in smf with 19.94% and 10.43% removal of hemicelluloses and lignin, respectively. ftir analysis suggested the ...201626720135
alkaline-sulfite pretreatment and use of surfactants during enzymatic hydrolysis to enhance ethanol production from sugarcane bagasse.sugarcane bagasse is a by-product from the sugar and ethanol industry which contains approximately 70 % of its dry mass composed by polysaccharides. to convert these polysaccharides into fuel ethanol it is necessary a pretreatment step to increase the enzymatic digestibility of the recalcitrant raw material. in this work, sugarcane bagasse was pretreated by an alkaline-sulfite chemithermomechanical process for increasing its enzymatic digestibility. na2so3 and naoh ratios were fixed at 2:1, and ...201626718203
physiological and transcriptional analyses of developmental stages along sugarcane leaf.sugarcane is one of the major crops worldwide. it is cultivated in over 100 countries on 22 million ha. the complex genetic architecture and the lack of a complete genomic sequence in sugarcane hamper the adoption of molecular approaches to study its physiology and to develop new varieties. investments on the development of new sugarcane varieties have been made to maximize sucrose yield, a trait dependent on photosynthetic capacity. however, detailed studies on sugarcane leaves are scarce. in t ...201526714767
enzymatic hydrolysis of pretreated sugarcane straw: kinetic study and semi-mechanistic modeling.although there are already commercial-scale productions of second generation (2g) ethanol, focusing efforts on process optimization can be of key importance to make the production cost-effective in large scale. in this scenario, mathematical models may be useful in design, scale-up, optimization, and control of bioreactors. for this reason, the aim of this work was to study the kinetics of the enzymatic hydrolysis of cellulose from sugarcane straw. experiments using hydrothermally pretreated sug ...201626701144
evaluation of lime and hydrothermal pretreatments for efficient enzymatic hydrolysis of raw sugarcane bagasse.ethanol production from sugarcane bagasse requires a pretreatment step to disrupt the cellulose-hemicellulose-lignin complex and to increase biomass digestibility, thus allowing the obtaining of high yields of fermentable sugars for the subsequent fermentation. hydrothermal and lime pretreatments have emerged as effective methods in preparing the lignocellulosic biomass for bioconversion. these pretreatments are advantageous because they can be performed under mild temperature and pressure condi ...201526633992
controlled release of drugs from cellulose acetate matrices produced from sugarcane bagasse: monitoring by square-wave voltammetry.in this paper, cellulose triacetate (cta) was produced from sugarcane bagasse and used as matrices for controlled release of paracetamol. symmetric and asymmetric membranes were obtained by formulations of cta/dichloromethane/drug and cta/dichloromethane/water/drug, respectively, and they were characterized by scanning electron microscopy (sem) and differential scanning calorimetry (dsc). different morphologies of membranes were observed by sem, and the incorporation of paracetamol was confirmed ...201626596497
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