| crystallization and preliminary x-ray analysis of carboxypeptidase 1 from thermus thermophilus. | carboxypeptidase 1 from the thermophilic eubacterium thermus thermophilus (tthcp1, 58 kda), a member of the m32 family of metallocarboxypeptidases, was crystallized by the sitting-drop vapour-diffusion method using peg 8000 as the precipitant. the crystals diffracted x-rays to beyond 2.6 a resolution using a synchrotron-radiation source. the crystals belonged to the orthorhombic space group c222(1), with unit-cell parameters a = 171.0, b = 231.6, c = 124.9 a. the crystal contains three molecules ... | 2004 | 15272172 |
| gram-positive rods prevailing in teeth with apical periodontitis undergoing root canal treatment. | to identify gram-positive rods from root canals of teeth with apical periodontitis and to examine their associations with other species. | 2004 | 15317560 |
| conservation of functional domain structure in bicarbonate-regulated "soluble" adenylyl cyclases in bacteria and eukaryotes. | soluble adenylyl cyclase (sac) is an evolutionarily conserved bicarbonate sensor. in mammals, it is responsible for bicarbonate-induced, camp-dependent processes in sperm required for fertilization and postulated to be involved in other bicarbonate- and carbon dioxide-dependent functions throughout the body. among eukaryotes, sac-like cyclases have been detected in mammals and in the fungi dictyostelium; these enzymes display extensive similarity extending through two cyclase catalytic domains a ... | 2004 | 15322879 |
| initial antimicrobial effect of controlled-release doxycycline in subgingival sites. | to determine the ability of a 10% doxycycline hyclate controlled-release polymer (atridox) to suppress periodontopathic bacteria when placed subgingivally following scaling and root planing (sc/rp). | 2004 | 15324352 |
| a new real time pcr (taqman pcr) system for detection of the16s rdna gene associated with fecal bacteria. | a recent pcr detection technique (taqman) based on a 3'-minor groove binder (mgb) probe was applied to the detection of fecal-dominant bacteria to assess fecal contamination in environmental samples. primers and probes used bacterial 16s ribosomal dna (16s rdna) as a gene marker and accurately defined with specificity a cluster of phylotypes within the gram-positive low gc division. this cluster of phylotypes, called fec1, corresponds to around 5% of human fecal microflora. fec1 clustered 16s rd ... | 2004 | 15325749 |
| dna-dna relatedness and phylogenetic positions of slackia exigua, slackia heliotrinireducens, eggerthella lenta, and other related bacteria. | recently, two asaccharolytic eubacterium species, eubacterium exiguum and eubacterium lentum, and peptostreptococcus heliotrinreducens have been reclassified as slackia exigua, eggerthella lenta and slackia heliotrinireducens in the novel genera on the basis of 16s rdna sequence analysis. but dna-dna relatedness among these species and other related bacteria have not been reported yet. dna-dna relatedness is the standard arbiter and the recommended method for the designation and evaluation of ne ... | 2004 | 15327650 |
| metabolic properties of eubacterium pyruvativorans, a ruminal 'hyper-ammonia-producing' anaerobe with metabolic properties analogous to those of clostridium kluyveri. | eubacterium pyruvativorans i-6(t) is a non-saccharolytic, amino-acid-fermenting anaerobe from the rumen, isolated by its ability to grow on pancreatic casein hydrolysate (pch) as sole c source. this study investigated its metabolic properties and its likely ecological niche. additional growth was supported by pyruvate, vinyl acetate, and, to a lesser extent, lactate and crotonate, and also by a mixture of amino acids (alanine, glycine, serine and threonine) predicted to be catabolized to pyruvat ... | 2004 | 15347751 |
| clinical and microbiological changes associated with the use of combined antimicrobial therapies to treat "refractory" periodontitis. | the present investigation examined clinical and microbial changes after a combined aggressive antimicrobial therapy in subjects identified as "refractory" to conventional periodontal therapy. | 2004 | 15367191 |
| validation of fluorescent in situ hybridization combined with flow cytometry for assessing interindividual variation in the composition of human fecal microflora during long-term storage of samples. | this work was conducted to assess the accuracy of in situ hybridization to show differences in human microflora composition between volunteers and to optimize the storage of fecal samples to allow delayed analysis of gut microflora composition in humans. fecal samples from 25 healthy subjects (14 women, 11 men aged 24-51) were collected. the samples were fixed in 4% paraformaldehyde (pfa) solution at 4 degrees c overnight and stored at -70 degrees c. twenty samples were analysed to quantify the ... | 2004 | 15369862 |
| the crystal structure of an est2 mutant unveils structural insights on the h group of the carboxylesterase/lipase family. | esterase 2 (est2) from the thermophilic eubacterium alicyclobacillus acidocaldarius is a thermostable serine hydrolase belonging to the h group of the esterase/lipase family. this enzyme hydrolyzes monoacylesters of different acyl-chain length and various compounds with industrial interest. est2 displays an optimal temperature at 70 degrees c and maximal activity with pnp-esters having acyl-chain bearing from six to eight carbon atoms. est2 mutants with different substrate specificity were also ... | 2004 | 15381425 |
| lactate-utilizing bacteria, isolated from human feces, that produce butyrate as a major fermentation product. | the microbial community of the human colon contains many bacteria that produce lactic acid, but lactate is normally detected only at low concentrations (<5 mm) in feces from healthy individuals. it is not clear, however, which bacteria are mainly responsible for lactate utilization in the human colon. here, bacteria able to utilize lactate and produce butyrate were identified among isolates obtained from 10(-8) dilutions of fecal samples from five different subjects. out of nine such strains ide ... | 2004 | 15466518 |
| cloning and expression of a phloretin hydrolase gene from eubacterium ramulus and characterization of the recombinant enzyme. | phloretin hydrolase catalyzes the hydrolytic c-c cleavage of phloretin to phloroglucinol and 3-(4-hydroxyphenyl)propionic acid during flavonoid degradation in eubacterium ramulus. the gene encoding the enzyme was cloned by screening a gene library for hydrolase activity. the insert of a clone conferring phloretin hydrolase activity was sequenced. sequence analysis revealed an open reading frame of 822 bp (phy), a putative promoter region, and a terminating stem-loop structure. the deduced amino ... | 2004 | 15466559 |
| detection frequency of periodontitis-associated bacteria by polymerase chain reaction in subgingival and supragingival plaque of periodontitis and healthy subjects. | the aim of this study was to compare the detection frequencies of 25 bacterial species in subgingival and supragingival plaque of 18 untreated periodontitis subjects and 12 periodontally healthy subjects. genomic dna was extracted from subgingival and supragingival plaque samples, and bacterial detection was performed by polymerase chain reaction of the 16s rrna genes. fourteen bacteria showed no relationship with periodontitis, and 11 of these 14 species were frequently detected (> or =50%) in ... | 2004 | 15491463 |
| ribosomal antibiotics: structural basis for resistance, synergism and selectivity. | various antibiotics bind to ribosomes at functionally relevant locations such as the peptidyl-transferase center (ptc) and the exit tunnel for nascent proteins. high-resolution structures of antibiotics bound to ribosomal particles from a eubacterium that is similar to pathogens and an archaeon that shares properties with eukaryotes are deciphering subtle differences in these highly conserved locations that lead to drug selectivity and thereby facilitate clinical usage. these structures also sho ... | 2004 | 15491801 |
| elongation factor ts from the antarctic eubacterium pseudoalteromonas haloplanktis tac 125: biochemical characterization and cloning of the encoding gene. | the elongation factor ts was isolated from the psychrophilic antarctic eubacterium pseudoalteromonas haloplanktis tac 125 strain (phef-ts), and its functional properties were studied. at 0 degrees c phef-ts enhanced the [(3)h]gdp/gdp exchange rate on the preformed phef-tu.[(3)h]gdp complex by 2 orders of magnitude even at very low tu:ts ratio, by lowering the energy of activation of the exchange reaction. phef-ts is a monomeric protein, and in solution it forms a stable dimeric complex with phef ... | 2004 | 15544346 |
| characterization of a 5'-polynucleotide kinase/3'-phosphatase from bacteriophage rm378. | a polynucleotide kinase from the thermophilic bacteriophage rm378 that infects the thermophilic eubacterium rhodothermus marinus was identified, expressed, and purified. this polynucleotide kinase was demonstrated to have a 5'-kinase domain as well as a 3'-phosphohydrolase domain. the rm378 polynucleotide kinase had limited sequence similarity to the 5'-kinase domain of the t4 bacteriophage polynucleotide kinase, but apparent homology was not evident within the 3'-phosphohydrolase domain. the do ... | 2004 | 15579472 |
| anaerobic, non-sporulating, gram-positive bacilli bacteraemia characterized by 16s rrna gene sequencing. | owing to the difficulties in identifying anaerobic, non-sporulating, gram-positive bacilli in clinical microbiology laboratories, the epidemiology and clinical spectrum of disease of many of these bacteria have been poorly understood. the application of 16s rrna gene sequencing in characterizing bacteraemia due to anaerobic, non-sporulating gram-positive bacilli during a 4-year period is described. the first case of olsenella uli bacteraemia, in a patient with acute cholangitis, is also reported ... | 2004 | 15585505 |
| effects of deoxynivalenol on general performance and electrophysiological properties of intestinal mucosa of broiler chickens. | a feeding trial was conducted to evaluate the effects of diets contaminated with deoxynivalenol (don on the performance of broilers and on the electro-physiological parameters of the gut. the control group was fed the starter and finisher diets without addition of don. another group of broilers was fed the starter and finisher diets with 10 mg/kg don, whereas another group was fed the don-contaminated diets supplemented with a microbial feed additive (eubacterium sp.). the diets were provided ad ... | 2004 | 15615008 |
| investigation of zinc-containing peptide deformylase from leptospira interrogans by x-ray absorption near-edge spectroscopy. | peptide deformylase (pdf, ec 3.5.1.27) is essential for the normal growth of eubacterium but not for mammalians. recently, pdf has been studied as a target for new antibiotics. its activity is strongly dependent on the bound metal ion. the crystallographic studies did not show any significant structural difference upon various bound metal ions. in this paper, x-ray absorption spectroscopy was employed to determine the local structure around the zinc ion of pdf from leptospira interrogans in dry ... | 2004 | 15616374 |
| protein-based biosensors for diabetic patients. | in this article we show the recent progress in the field of glucose sensing based on the utilization of enzymes and proteins as probes for stable and non-consuming fluorescence biosensors. we developed a new methodology for glucose sensing using inactive forms of enzymes such as the glucose oxidase from aspergillus niger, the glucose dehydrogenase from the thermophilic microorganism thermoplasma acidophilum, and the glucokinase from the thermophilic eubacterium bacillus stearothermophilus. gluco ... | 2004 | 15617257 |
| characterization of aquifex aeolicus rnase e/g. | the rnase e/g homologue from the thermophilic eubacterium aquifex aeolicus has been overexpressed in escherichia coli, purified, and characterized in vitro. we show that a. aeolicus rnase e/g has a temperature-dependent, endoribonucleolytic activity. the enzyme site-specifically cleaves oligonucleotides and structured rnas at locations that are partly overlapping or completely different when compared to the positions of e. coli rnase e and rnase g cleavage sites. the efficiency of cleavage by a. ... | 2005 | 15629127 |
| monitoring of antibiotic-induced alterations in the human intestinal microflora and detection of probiotic strains by use of terminal restriction fragment length polymorphism. | terminal restriction fragment length polymorphism (t-rflp) was investigated as a tool for monitoring the human intestinal microflora during antibiotic treatment and during ingestion of a probiotic product. fecal samples from eight healthy volunteers were taken before, during, and after administration of clindamycin. during treatment, four subjects were given a probiotic, and four subjects were given a placebo. changes in the microbial intestinal community composition and relative abundance of sp ... | 2005 | 15640226 |
| differences in the subgingival microbiota of swedish and usa subjects who were periodontally healthy or exhibited minimal periodontal disease. | previous studies have shown differences in the mean proportions of subgingival species in samples from periodontitis subjects in different countries, which may relate to differences in diet, genetics, disease susceptibility and manifestation. the purpose of the present investigation was to determine whether there were differences in the subgingival microbiotas of swedish and american subjects who exhibited periodontal health or minimal periodontal disease. | 2005 | 15642056 |
| isolation and characterization of a thermostable rna ligase 1 from a thermus scotoductus bacteriophage ts2126 with good single-stranded dna ligation properties. | we have recently sequenced the genome of a novel thermophilic bacteriophage designated as ts2126 that infects the thermophilic eubacterium thermus scotoductus. one of the annotated open reading frames (orfs) shows homology to t4 rna ligase 1, an enzyme of great importance in molecular biology, owing to its ability to ligate single-stranded nucleic acids. the orf was cloned, and recombinant protein was expressed, purified and characterized. the recombinant enzyme ligates single-stranded nucleic a ... | 2005 | 15642699 |
| crystal structure of the phosphoenolpyruvate-binding enzyme i-domain from the thermoanaerobacter tengcongensis pep: sugar phosphotransferase system (pts). | enzyme i (ei), the first component of the phosphoenolpyruvate (pep):sugar phosphotransferase system (pts), consists of an n-terminal protein-binding domain (ein) and a c-terminal pep-binding domain (eic). ei transfers phosphate from pep by double displacement via a histidine residue on ein to the general phosphoryl carrier protein hpr. here, we report the 1.82a crystal structure of the homodimeric eic domain from thermoanaerobacter tengcongensis, a saccharolytic eubacterium that grows optimally ... | 2004 | 15670601 |
| molecular analysis of microbial diversity in advanced caries. | real-time pcr analysis of the total bacterial load in advanced carious lesions has shown that the total load exceeds the number of cultivable bacteria. this suggests that an unresolved complexity exists in bacteria associated with advanced caries. in this report, the profile of the microflora of carious dentine was explored by using dna extracted from 10 lesions selected on the basis of comparable total microbial load and on the relative abundance of prevotella spp. using universal primers for t ... | 2005 | 15695690 |
| [screening of natural compounds with hypolipidemic activity]. | in the screening programme for natural hypolipidemic compounds 702 strains of soil microorganisms were tested and 25 of them were selected because of their ability to produce compounds inhibiting sterol synthesis in hep g2 hepatoma cells. the compounds were estimated in the microbiological model with tolypocladium inflatum 106 as the test microbe. the 2nd stage of the screening resulted in isolation of 13 strains producing compounds with high hypolipidemic activity, analogous to or higher than t ... | 2004 | 15727139 |
| degradation of neohesperidin dihydrochalcone by human intestinal bacteria. | the degradation of neohesperidin dihydrochalcone by human intestinal microbiota was studied in vitro. human fecal slurries converted neohesperidin dihydrochalcone anoxically to 3-(3-hydroxy-4-methoxyphenyl)propionic acid or 3-(3,4-dihydroxyphenyl)propionic acid. two transient intermediates were identified as hesperetin dihydrochalcone 4'-beta-d-glucoside and hesperetin dihydrochalcone. these metabolites suggest that neohesperidin dihydrochalcone is first deglycosylated to hesperetin dihydrochalc ... | 2005 | 15740074 |
| differential dna binding and protection by dimeric and dodecameric forms of the ferritin homolog dps from deinococcus radiodurans. | bacterial iron storage proteins such as ferritin serve as intracellular iron reserves. members of the dna protection during starvation (dps) family of proteins are structurally related to ferritins, and their function is to protect the genome from iron-induced free radical damage. some members of the dps family bind dna and are thought to do so only as fully assembled dodecamers. we present the cloning and characterization of a dps homolog encoded by the radiation-resistant eubacterium deinococc ... | 2005 | 15755446 |
| guggenheimella bovis gen. nov., sp. nov., isolated from lesions of bovine dermatitis digitalis. | dermatitis digitalis is an economically important ulcerative disease of undetermined aetiology affecting the hooves of cattle. material was examined from two independent cases of this disease in switzerland. cultures from the advancing front of both lesions yielded large numbers of closely related short, mesophilic, non-motile, non-spore-forming, anaerobic, proteolytic, gram-positive rods. the 16s rrna gene sequences of strains omz 913(t) and omz 915 were identical and indicate tindallia magadie ... | 2005 | 15774641 |
| the beneficial effects of microflora, especially obligate anaerobes, and their products on the colonic environment in inflammatory bowel disease. | because intestinal microflora play a pivotal role in the development of inflammatory bowel disease (ibd), there is currently some interest in alternating the composition of the microflora toward a potentially more remedial community. this paper summarizes the clinical and experimental efficacy of the manipulation of microflora by the use of antibiotics, probiotics, and prebiotics in ibd. germinated barley foodstuff (gbf) is a prebiotic whose unique characteristics make it highly suitable for app ... | 2005 | 15777254 |
| mucosa-associated bacteria in ulcerative colitis before and after antibiotic combination therapy. | we proposed that fusobacterium varium is one of the causative agents in ulcerative colitis. | 2005 | 15813838 |
| decoding the genomic tree of life. | genomes hold within them the record of the evolution of life on earth. but genome fusions and horizontal gene transfer (hgt) seem to have obscured sufficiently the gene sequence record such that it is difficult to reconstruct the phylogenetic tree of life. hgt among prokaryotes is not random, however. some genes (informational genes) are more difficult to transfer than others (operational genes). furthermore, environmental, metabolic, and genetic differences among organisms restrict hgt, so that ... | 2005 | 15851667 |
| [the structure-function relationship of thermostable beta-glycosidase from the thermophilic eubacterium thermus nonproteolyticus hg102]. | beta-glycosidase (tngly) from the thermophilic eubacterium thermus nonproteolyticus hg102, which is a thermostable monomeric protein and adopts the (beta/alpha)8 barrel fold, is an excellent model system to be investigated for the thermostable mechanism, activity and substrate specificity. here, based on the analysis of structural basis for thermostability of tngly (wang et al, 2003) and comparison of other proteins structure of homofamily, glu164 and glu338 may act as proton donor and nucleophi ... | 2005 | 15859334 |
| a four-subunit cytochrome bc(1) complex complements the respiratory chain of thermus thermophilus. | several components of the respiratory chain of the eubacterium thermus thermophilus have previously been characterized to various extent, while no conclusive evidence for a cytochrome bc(1) complex has been obtained. here, we show that four consecutive genes encoding cytochrome bc(1) subunits are organized in an operon-like structure termed fbccxfb. the four gene products are identified as genuine subunits of a cytochrome bc(1) complex isolated from membranes of t. thermophilus. while both the c ... | 2005 | 15869739 |
| effects of macromolecular impurities and of crystallization method on the quality of eubacterial aspartyl-trna synthetase crystals. | although macromolecular purity is thought to be essential for the growth of flawless protein crystals, only a few studies have investigated how contaminants alter the crystallization process and crystal quality. likewise, the outcome of a crystallization process may vary with the crystallization method. here, it is reported how these two variables affect the crystallogenesis of aspartyl-trna synthetase from the eubacterium thermus thermophilus. this homodimeric enzyme (mr=130,000) possesses a mu ... | 2005 | 15930641 |
| microarrays complement culture methods for identification of bacteria in endodontic infections. | the aim of this study was to investigate the microbial composition of necrotic root canals using culture methods and microarray technology. twenty uniradicular teeth with radiographic evidence of periapical bone loss and with no previous endodontic treatment were selected for this study. for molecular diagnosis a dna chip with 20 different species-specific, 16s-rdna-directed catcher probes was used. the microorganisms most frequently detected by the dna chip were: micromonas micros, fusobacteriu ... | 2005 | 15943771 |
| a crevice adjoining the ribosome tunnel: hints for cotranslational folding. | rna protection experiments and the crystal structure of a complex of the large ribosomal subunit from the eubacterium deinococcus radiodurans with rapamycin, a polyketide compound resembling macrolides and ketolides, showed that rapamycin binds to a crevice located at the boundaries of the nascent protein exit tunnel, near its entrance. at this location rapamycin cannot occlude the ribosome exit tunnel, consistent with its failure to act as a ribosomal antibiotic drug. in accord with recent bioc ... | 2005 | 15943964 |
| design and validation of 16s rrna probes to enumerate members of the clostridium leptum subgroup in human faecal microbiota. | among human faecal bacteria, many members of the clostridium leptum subgroup are fibrolytic and butyrate producing microorganisms thereby contributing to processes important to colonic health. yet this phylogenetic subgroup remains poorly described to date. to improve detection and description of members of the c. leptum subgroup, the clep 866 group probe was developed. its association with probes targeting the clostridium viride cluster (cvir 1414) and eubacterium desmolans species (edes 635) a ... | 2005 | 15946290 |
| analysis of the large bowel microbiota of colitic mice using pcr/dgge. | to test combined polymerase chain reaction amplification of 16s rrna gene sequences and denaturing gradient gel electrophoresis (pcr/dgge) as an analytical method to investigate the composition of the large bowel microbiota of mice during the development of colitis. | 2005 | 15960751 |
| modulation of the fecal microbiota by the intake of a lactobacillus johnsonii la1-containing product in human volunteers. | lactobacillus johnsonii la1 (la1) is a probiotic strain capable of stimulating the immune system of the host and interfering with gastrointestinal pathogens. this study evaluates how the ingestion of different amounts of la1 influences the main bacterial populations of the fecal microbiota. eight asymptomatic volunteers participated in the study. after a basal period, they ingested daily 100 ml of a product containing 10(8) cfu ml(-1) of la1 during the first week, 200 ml during the second week a ... | 2005 | 15970400 |
| monitoring of microbial community structure and succession in the biohydrogen production reactor by denaturing gradient gel electrophoresis (dgge). | to study the structure of microbial communities in the biological hydrogen production reactor and determine the ecological function of hydrogen producing bacteria, anaerobic sludge was obtained from the continuous stirred tank reactor (cstr) in different periods of time, and the diversity and dynamics of microbial communities were investigated by denaturing gradient gel electrophoresis (dgge). the results of dgge demonstrated that an obvious shift of microbial population happened from the beginn ... | 2005 | 15986888 |
| spatial organization and composition of the mucosal flora in patients with inflammatory bowel disease. | the composition and spatial organization of the mucosal flora in biopsy specimens from patients with inflammatory bowel disease (ibd; either crohn's disease or ulcerative colitis), self-limiting colitis, irritable-bowel syndrome (ibs), and healthy controls were investigated by using a broad range of fluorescent bacterial group-specific rrna-targeted oligonucleotide probes. each group included 20 subjects. ten patients who had ibd and who were being treated with antibiotics were also studied. use ... | 2005 | 16000463 |
| the maintenance in the oral cavity of children of tetracycline-resistant bacteria and the genes encoding such resistance. | to investigate the maintenance of tetracycline-resistant oral bacteria and the genes encoding tetracycline resistance in these bacteria in children (aged 4--6 years) over a period of 12 months. | 2005 | 16027144 |
| cutaneous abscess due to eubacterium lentum in injection drug user: a case report and review of the literature. | we described the first case, to the best of our knowledge, of cutaneous abscess due to eubacterium lentum in a parenteral drug user, after complete fracture of the right femor. the case underlines the importance of carefully performed microbiological tests, due to the peculiar cultural needs of the micro-organism. | 2005 | 16038756 |
| searching for intermediates in the carbon skeleton rearrangement of 2-methyleneglutarate to (r)-3-methylitaconate catalyzed by coenzyme b12-dependent 2-methyleneglutarate mutase from eubacterium barkeri. | coenzyme b(12)-dependent 2-methyleneglutarate mutase from the strict anaerobe eubacterium barkeri catalyzes the equilibration of 2-methyleneglutarate with (r)-3-methylitaconate. proteins with mutations in the highly conserved coenzyme binding-motif dxh(x)(2)g(x)(41)gg (d483n and h485q) exhibited decreased substrate turnover by 2000-fold and >4000-fold, respectively. these findings are consistent with the notion of h485 hydrogen-bonded to d483 being the lower axial ligand of adenosylcobalamin in ... | 2005 | 16060663 |
| activation of proestrogens from hops (humulus lupulus l.) by intestinal microbiota; conversion of isoxanthohumol into 8-prenylnaringenin. | hop, an essential ingredient in most beers, contains a number of prenylflavonoids, among which 8-prenylnaringenin (8-pn) would be the most potent phytoestrogen currently known. although a number of health effects are attributed to these compounds, only a few reports are available about the bioavailability of prenylflavonoids and the transformation potency of the intestinal microbial community. to test these transformations, four fecal samples were incubated with xanthohumol, isoxanthohumol (ix), ... | 2005 | 16076107 |
| phylogenetic analysis of pasteuria penetrans by use of multiple genetic loci. | pasteuria penetrans is a gram-positive, endospore-forming eubacterium that apparently is a member of the bacillus-clostridium clade. it is an obligate parasite of root knot nematodes (meloidogyne spp.) and preferentially grows on the developing ovaries, inhibiting reproduction. root knot nematodes are devastating root pests of economically important crop plants and are difficult to control. consequently, p. penetrans has long been recognized as a potential biocontrol agent for root knot nematode ... | 2005 | 16077116 |
| toxicity of methoprene as assessed by the use of a model microorganism. | methoprene is an insect juvenile growth hormone mimic, commonly used as a pesticide. although widely used for the control of several pests, toxic effects on organisms of different phyla have been reported. these events triggered studies to clarify the mechanisms of toxicity of this insecticide putatively involved in ecological issues. here we show the effect of methoprene on the normal cell growth and viability of a strain of the thermophilic eubacterium bacillus stearothermophilus, previously u ... | 2005 | 16081242 |
| identification of candidate periodontal pathogens and beneficial species by quantitative 16s clonal analysis. | most studies of the bacterial etiology of periodontitis have used either culture-based or targeted dna approaches, and so it is likely that pathogens remain undiscovered. the purpose of this study was to use culture-independent, quantitative analysis of biofilms associated with chronic periodontitis and periodontal health to identify pathogens and beneficial species. samples from subjects with periodontitis and controls were analyzed using ribosomal 16s cloning and sequencing. several genera, ma ... | 2005 | 16081935 |
| substrate specificity and transglycosylation catalyzed by a thermostable beta-glucosidase from marine hyperthermophile thermotoga neapolitana. | the gene encoding beta-glucosidase of the marine hyperthermophilic eubacterium thermotoga neapolitana (bgla) was subcloned and expressed in escherichia coli. the recombinant bgla (rbgla) was efficiently purified by heat treatment at 75 degrees c, and a ni-nta affinity chromatography and its molecular mass were determined to be 56.2 kda by mass spectrometry (ms). at 100 degrees c, the enzyme showed more than 94% of its optimal activity. the half-life of the enzyme was 3.6 h and 12 min at 100 and ... | 2005 | 16082555 |
| genetic diversity of viable, injured, and dead fecal bacteria assessed by fluorescence-activated cell sorting and 16s rrna gene analysis. | a novel approach combining a flow cytometric in situ viability assay with 16s rrna gene analysis was used to study the relationship between diversity and activity of the fecal microbiota. simultaneous staining with propidium iodide (pi) and syto bc provided clear discrimination between intact cells (49%), injured or damaged cells (19%), and dead cells (32%). the three subpopulations were sorted and characterized by denaturing gradient gel electrophoresis (dgge) of 16s rrna gene amplicons obtaine ... | 2005 | 16085863 |
| structure of trigger factor binding domain in biologically homologous complex with eubacterial ribosome reveals its chaperone action. | trigger factor (tf), the first chaperone in eubacteria to encounter the emerging nascent chain, binds to the large ribosomal subunit in the vicinity of the protein exit tunnel opening and forms a sheltered folding space. here, we present the 3.5-a crystal structure of the physiological complex of the large ribosomal subunit from the eubacterium deinococcus radiodurans with the n-terminal domain of tf (tfa) from the same organism. for anchoring, tfa exploits a small ribosomal surface area in the ... | 2005 | 16091460 |
| structural and functional reconstruction in situ of the [cusmoo2] active site of carbon monoxide dehydrogenase from the carbon monoxide oxidizing eubacterium oligotropha carboxidovorans. | carbon monoxide dehydrogenase from the bacterium oligotropha carboxidovorans catalyzes the oxidation of co to co(2) at a unique [cusmoo(2)] cluster. in the bacteria the cluster is assembled post-translational. the integration of s, and particularly of cu, is rate limiting in vivo, which leads to co dehydrogenase preparations containing the mature and fully functional enzyme along with forms of the enzyme deficient in one or both of these elements. the active sites of mature and immature forms of ... | 2005 | 16091936 |
| domain reorientation and induced fit upon rna binding: solution structure and dynamics of ribosomal protein l11 from thermotoga maritima. | l11, a protein of the large ribosomal subunit, binds to a highly conserved domain of 23s rrna and mediates ribosomal gtpase activity. its c-terminal domain is the main determinant for rrna binding, whereas its n-terminal domain plays only a limited role in rna binding. the n-terminal domain is thought to be involved in interactions with elongation and release factors as well as with the antibiotics thiostrepton and micrococcin. this report presents the nmr solution structure of the full-length l ... | 2005 | 16094695 |
| xanthorhodopsin: a proton pump with a light-harvesting carotenoid antenna. | energy transfer from light-harvesting carotenoids to chlorophyll is common in photosynthesis, but such antenna pigments have not been observed in retinal-based ion pumps and photoreceptors. here we describe xanthorhodopsin, a proton-pumping retinal protein/carotenoid complex in the eubacterium salinibacter ruber. the wavelength dependence of the rate of pumping and difference absorption spectra measured under a variety of conditions indicate that this protein contains two chromophores, retinal a ... | 2005 | 16179480 |
| metabolic activation of bladder procarcinogens, 2-aminofluorene, 4-aminobiphenyl, and benzidine by pseudomonas aeruginosa and other human endogenous bacteria. | pseudomonas aeruginosa, an opportunistic pathogen of the human urinary tract, and other selected human endogenous bacteria were investigated for metabolic activation of the bladder procarcinogens, 2-aminofluorene (2-af), 4-aminobiphenyl (4-ab), and benzidine (bz). the cell-free extracts of pseudomonas aeruginosa, escherichia coli, enterobacter aerogenes, proteus mirabilis, proteus vulgaris, staphylococcus epidermidis, staphylococcus saprophyticus, klebsiella pneumoniae, and intestinal anaerobes, ... | 2006 | 16203120 |
| cloning, sequencing, and expression of a eubacterium cellulosolvens 5 gene encoding an endoglucanase (cel5a) with novel carbohydrate-binding modules, and properties of cel5a. | a novel eubacterium cellulosolvens 5 gene encoding an endoglucanase (cel5a) was cloned and expressed in escherichia coli, and its enzymatic properties were characterized. the cel5a gene consists of a 3,444-bp open reading frame and encodes a 1,148-amino-acid protein with a molecular mass of 127,047 da. cel5a is a modular enzyme consisting of an n-terminal signal peptide, two glycosyl hydrolase family 5 catalytic modules, two novel carbohydrate-binding modules (cbms), two linker sequences, and a ... | 2005 | 16204489 |
| cloning, nucleotide sequence and module structure of the gene encoding the cellulose-binding protein b (cbpb) of eubacterium cellulosolvens 5. | the nucleotide sequence of the gene encoding the cellulose-binding protein b (cbpb) of eubacterium cellulosolvens 5 was determined. the gene consists of an open reading frame of 3,429 nucleotides. the deduced amino acid sequence of cbpb contained one module highly similar to a catalytic module of glycosyl hydrolase family 9 (ghf9), one module partially similar to a family 3 carbohydrate-binding module (cbm3), two linkers, one module similar to a cbm of cellulose-binding protein a (cbpa) from e. ... | 2005 | 16205028 |
| [soft tissue infections in oral, maxillofacial, and plastic surgery. bacterial spectra and antibiotics]. | soft tissue infections in the maxillofacial region are mainly caused by staphylococcus aureus, pseudomonas aeruginosa, and escherichia coli but also by members of the genera enterococcus, klebsiella, and enterobacter, respectively. | 2005 | 16228187 |
| the home stretch, a first analysis of the nearly completed genome of rhodobacter sphaeroides 2.4.1. | rhodobacter sphaeroides 2.4.1 is an alpha-3 purple nonsulfur eubacterium with an extensive metabolic repertoire. under anaerobic conditions, it is able to grow by photosynthesis, respiration and fermentation. photosynthesis may be photoheterotrophic using organic compounds as both a carbon and a reducing source, or photoautotrophic using carbon dioxide as the sole carbon source and hydrogen as the source of reducing power. in addition, r. sphaeroides can grow both chemoheterotrophically and chem ... | 2001 | 16228360 |
| formulation of defined media for carbon monoxide fermentation by eubacterium limosum kist612 and the growth characteristics of the bacterium. | phosphate-buffered (pbbm) and carbonate-buffered (cbbm) basal media were used in the formulation of defined media for the cultivation of eubacterium limosum kist612 with carbon monoxide (co) as the sole energy source. the bacterium was adapted to the minimal media by sequential passage in media containing casamino acids and those containing ammonium chloride in the place of yeast extract. biological growth was slower with a lower growth yield in the defined minimal media than in pbbm or cbbm. mo ... | 1999 | 16232686 |
| analysis of the methionine biosynthetic pathway in the extremely thermophilic eubacterium thermus thermophilus. | four dna fragments that could rescue the mutations of four met- mutants were cloned from thermus thermophilus hb27 and their complete nucleotide sequences were determined. two of the four fragments respectively contained the greater parts of the metf and meth genes, the predicted amino acid sequences of which showed identities of 30.8% and 32.7% with 5,10-methylenetetrahydrofolate reductase (ec 1.7.99.5) and vitamin b12-dependent homocysteine transmethylase (ec 2.1.1.13) of escherichia coli. the ... | 2000 | 16232856 |
| synthesis of methyl ursodeoxycholate via microbial reduction of methyl 7-ketolithocholate with eubacterium aerofaciens jcm 7790 grown on two kinds of carbon and hydride sources, glucose and mannitol. | eubacterium aerofaciens jcm 7790, which produces 7beta-hydroxysteroid dehydrogenase [7beta-hsdh], efficiently catalyzed the reduction of methyl 7-ketolithocholate [me-7klca] to afford methyl ursodeoxycholate [me-udca] in an anaerobic interface bioreactor. for the high accumulation of me-udca, the best supplement of carbon and hydride source to the abcm medium (glucose content, 0.3 wt.%) was screened because glucose strongly repressed 7beta-hsdh production. the supplementation of cellobiose, mann ... | 2001 | 16232971 |
| preparation of methyl ursodeoxycholate via microbial reduction of methyl 7-ketolithocholate in an anaerobic interface bioreactor. | an interface bioreactor, which is a device for the microbial transformation of water-insoluble substrates, was applied to an anaerobic bioconversion for the first time. methyl 7-ketolithocholate [me-7klca] was reduced with the human intestinal bacterium eubacterium aerofaciens jcm 7790 in a convenient anaerobic interface bioreactor using a nutrient agar plate placed in a gaspak pouch. the resulting methyl ursodeoxycholate [me-udca] is a precursor of ursodeoxycholic acid, which is used as a chole ... | 2001 | 16232975 |
| preparation of methyl ursodeoxycholate in an interface bioreactor: use of a mixed solvent system to increase the solubilities of substrate and product. | a mixed solvent system was used as the reaction solvent of an interface bioreactor for the first time. the solvent, dodecane/2-ethylhexyl acetate (4:6), exhibited excellent efficiency in the preparation of methyl ursodeoxycholate with eubacterium aerofaciens jcm 7790. | 2001 | 16233065 |
| [bacterial spectra and antibiotics in odontogenic infections. renaissance of the penicillins?]. | the role played by odontogenic infection in dental, oral, and maxillofacial surgery is not to be underestimated even at the present time. an extensive, standardized, prospective study was performed with the intention of verifying the bacterial spectrum of odontogenic infections to evaluate antibiotic sensitivity. | 2005 | 16261393 |
| mitochondrial genetics of aging: intergenomic conflict resolution. | mitochondria are the organelles of aerobic respiration. they consume the oxygen we breathe to stay alive and generate energy for cells to function. but oxygen can be dangerous. indeed, mitochondria generate the majority of reactive oxygen species that are prime suspects among the causes of aging. mitochondria have been influential elements of evolving eukaryotic cells for perhaps 2 billion years, since a eubacterium fused with an archaebacterium. the picture that has emerged from this long histo ... | 2005 | 16282285 |
| oxalate degrading bacteria: new treatment option for patients with primary and secondary hyperoxaluria? | current treatment options in patients with primary and secondary hyperoxaluria are limited and do not always lead to sufficient reduction in urinary oxalate excretion. intestinal oxalate degrading bacteria are capable of degrading oxalate to co(2) and formate, the latter being further metabolized and excreted via the feces. it is speculated, that both endogenously produced, as well as dietary oxalate can be significantly removed via the intestinal tract. oxalobacter formigenes, an obligate anaer ... | 2005 | 16284877 |
| phylogenetic analysis of tce-dechlorinating consortia enriched on a variety of electron donors. | two rapidly fermented electron donors, lactate and methanol, and two slowly fermented electron donors, propionate and butyrate, were selected for enrichment studies to evaluate the characteristics of anaerobic microbial consortia that reductively dechlorinate tce to ethene. each electron donor enrichment subculture demonstrated the ability to dechlorinate tce to ethene through several serial transfers. microbial community analyses based upon 16s rdna, including terminal restriction fragment leng ... | 2005 | 16294874 |
| the effect of haart on salivary microbiota in the women's interagency hiv study (wihs). | study the prevalence of potentially pathogenic microorganisms in saliva of hiv-positive women in the women's interagency hiv study. | 2005 | 16301151 |
| isoflavones and functional foods alter the dominant intestinal microbiota in postmenopausal women. | dietary phytoestrogens, such as isoflavones, are used as food additives to prevent menopause-related disorders. in addition to other factors, their bioavailability strongly depends on the activity of intestinal bacteria but the underlying interactions remain poorly understood. a randomized, double-blind, placebo-controlled study was undertaken with 39 postmenopausal women to characterize changes in the dominant microbial communities of the intestinal tract after 2 mo of isoflavone supplementatio ... | 2005 | 16317121 |
| characterisation of the microbial diversity in a pig manure storage pit using small subunit rdna sequence analysis. | the microbial community structure of pig manure slurry (pms) was determined with comparative analysis of 202 bacterial, 44 archaeal and 33 eukaryotic small subunit (ssu) rdna partial sequences. based on a criterion of 97% of sequence similarity, the phylogenetic analyses revealed a total of 108, eight and five phylotypes for the bacteria, archaea and eukarya lineages, respectively. only 36% of the bacterial phylotypes were closely related (>or=97% similarity) to any previously known sequence in ... | 2004 | 16329909 |
| in vitro determination of prebiotic properties of oligosaccharides derived from an orange juice manufacturing by-product stream. | fermentation properties of oligosaccharides derived from orange peel pectin were assessed in mixed fecal bacterial culture. the orange peel oligosaccharide fraction contained glucose in addition to rhamnogalacturonan and xylogalacturonan pectic oligosaccharides. twenty-four-hour, temperature- and ph-controlled, stirred anaerobic fecal batch cultures were used to determine the effects that oligosaccharides derived from orange products had on the composition of the fecal microbiota. the effects we ... | 2005 | 16332825 |
| the role of 16s rrna gene sequencing in identification of microorganisms misidentified by conventional methods. | traditional methods for microbial identification require the recognition of differences in morphology, growth, enzymatic activity, and metabolism to define genera and species. full and partial 16s rrna gene sequencing methods have emerged as useful tools for identifying phenotypically aberrant microorganisms. we report on three bacterial blood isolates from three different college of american pathologists-certified laboratories that were referred to arup laboratories for definitive identificatio ... | 2005 | 16333109 |
| functional identification of an 8-oxoguanine specific endonuclease from thermotoga maritima. | to date, no 8-oxoguanine-specific endonuclease-coding gene has been identified in thermotoga maritima of the order thermotogales, although its entire genome has been deciphered. however, the hypothetical protein tm1821 from t. maritima, has a helix-hairpin-helix motif that is considered to be important for dna binding and catalytic activity. here, tm1821 was overexpressed in escherichia coli and purified using ni-nta affinity chromatography, protease digestion, and gel filtration. tm1821 protein ... | 2005 | 16336782 |
| changes in lactate-producing and lactate-utilizing bacteria in relation to ph in the rumen of sheep during stepwise adaptation to a high-concentrate diet. | changes in the numbers and types of lactate-producing and lactate-utilizing bacteria in the rumen of sheep were followed during stepwise adaptation from a low- to a high-concentrate diet. the mean numbers of bacteria increased after each change in diet when increasing amounts of maize grain were substituted for maize stover. a surge in number of amylolytic bacteria always preceded an increase in lactate-utilizing bacteria, and with the final diet containing 71% grain and molasses the two groups ... | 1979 | 16345431 |
| syntrophic association by cocultures of the methanol- and co(2)-h(2)-utilizing species eubacterium limosum and pectin-fermenting lachnospira multiparus during growth in a pectin medium. | lachnospira multiparus grew very well in an anaerobic 0.2% pectin medium, whereas eubacterium limosum, which utilizes methanol, h(2)-co(2), and lactate, did not. cocultures of the two species grew at a somewhat more rapid growth rate than did l. multiparus alone and almost doubled the amount of growth as measured by optical density. in model experiments with cultures transferred once a day with a 2-day retention time, l. multiparus produced mainly acetate, methanol, ethanol, formate, lactate, co ... | 1981 | 16345811 |
| formation of n,n-dimethylglycine, acetic acid, and butyric acid from betaine by eubacterium limosum. | two bacterial strains that grow anaerobically on betaine were isolated from enrichment cultures and identified as strains of eubacterium limosum. in a mineral medium supplemented with yeast extract and casitone, the doubling time of e. limosum strain 11a on betaine was 6 h at 37 degrees c. the molar growth yield amounted to 9 g of dry cell mass per mol. betaine was fermented in accordance with the following equation: 7 betaine + 2 co(2) --> 7 n,n-dimethylglycine + 1.5 acetate + 1.5 butyrate. e. ... | 1981 | 16345842 |
| characterization of bacteria from a swine manure digester. | one-hundred thirty bacteria isolated from a swine manure digester were predominately gram-positive anaerobes which were tentatively classified into the following genera: peptostreptococcus, eubacterium, bacteroides, lactobacillus, peptococcus, clostridium, and streptococcus plus two unidentified groups. the major fermentation products formed by these organisms included acetate, propionate, succinate, lactate, and ethanol, singly or in various combinations. acetate was the sole end product of sev ... | 1982 | 16345916 |
| growth of eubacterium limosum with carbon monoxide as the energy source. | eubacterium limosum grew with co as the sole source of energy and formed acetate and co(2) as the major products. the generation time on co was 7 h. uninhibited growth occurred in cultures containing 50% co or less, but growth occurred at all concentrations tested (i.e., up to 75% co). the ph optimum for growth was 7.0 to 7.2, whereas growth was poor at a ph below 6.7. co(2) stimulated growth on co. co was preferentially utilized when both co and h(2) were present. | 1982 | 16345931 |
| biological and abiological sulfur reduction at high temperatures. | reduction of elemental sulfur was studied in the presence and absencè of thermophilic sulfur-reducing bacteria, at temperatures ranging from 65 to 110 degrees c, in anoxic artificial seawater media. above 80 degrees c, significant amounts of sulfide were produced abiologically at linear rates, presumably by the disproportionation of sulfur. these rates increased with increasing temperature and ph and were enhanced by yeast extract. in the same medium, the sulfur respiration of two recent thermop ... | 1985 | 16346781 |
| biotransformation of unsaturated long-chain fatty acids by eubacterium lentum. | eubacterium lentum (33 strains) isomerized the 12-cis double bond of c(18) fatty acids with cis double bonds at c-9 and c-12 into an 11-trans double bond before reduction of the 9-cis double bond. the 14-cis double bond of homo-gamma-linolenic acid was isomerized by 29 strains into a 13-trans double bond. the same strains isomerized the 14-cis double bond of arachidonic acid into a 13-trans double bond and then isomerized the 8-cis double bond into a 7-trans double bond; the 13-cis double bond o ... | 1986 | 16347014 |
| a new sulfur-reducing, extremely thermophilic eubacterium from a submarine thermal vent. | a newly described bacterial isolate, designated strain ns-e, differs from presently known extremely thermophilic bacteria in various characteristics. it is a strictly heterotrophic eubacterium of marine origin and has a temperature range for growth of 50 to 95 degrees c with an optimum at 77 degrees c and a ph of 7.5. its dna base composition is 41.3 mol% guanine + cytosine. it is obligately anaerobic, utilizes various sugars as well as yeast extract, and reduces elemental sulfur facultatively t ... | 1986 | 16347075 |
| magnesium and manganese content of halophilic bacteria. | magnesium and manganese contents were measured by atomic absorption spectrophotometry in bacteria of several halophilic levels, in vibrio costicola, a moderately halophilic eubacterium growing in 1 m nacl, halobacterium volcanii, a halophilic archaebacterium growing in 2.5 m nacl, halobacterium cutirubrum, an extremely halophilic archaebacterium growing in 4 m nacl, and escherichia coli, a nonhalophilic eubacterium growing in 0.17 m nacl. magnesium and manganese contents varied with the growth p ... | 1986 | 16347151 |
| high-affinity vanadate transport system in the cyanobacterium anabaena variabilis atcc 29413. | high-affinity vanadate transport systems have not heretofore been identified in any organism. anabaena variabilis, which can fix nitrogen by using an alternative v-dependent nitrogenase, transported vanadate well. the concentration of vanadate giving half-maximum v-nitrogenase activity when added to v-starved cells was about 3 x 10(-9) m. the genes for an abc-type vanadate transport system, vupabc, were found in a. variabilis about 5 kb from the major cluster of genes encoding the v-nitrogenase, ... | 2006 | 16385036 |
| assessment of the microbial community in a constructed wetland that receives acid coal mine drainage. | constructed wetlands are used to treat acid drainage from surface or underground coal mines. however, little is known about the microbial communities in the receiving wetland cells. the purpose of this work was to characterize the microbial population present in a wetland that was receiving acid coal mine drainage (amd). samples were collected from the oxic sediment zone of a constructed wetland cell in southeastern ohio that was treating acid drainage from an underground coal mine seep. samples ... | 2006 | 16400536 |
| studies on the effect of system retention time on bacterial populations colonizing a three-stage continuous culture model of the human large gut using fish techniques. | fluorescence in situ hybridization was used to quantitate bacteria growing in a three-stage continuous culture system inoculated with human faeces, operated at two system retention times (60 and 20 h). twenty-three different 16s rrna gene oligonucleotide probes of varying specificities were used to detect bacteria. organisms belonging to genera bacteroides and bifidobacterium, together with the eubacterium rectale/clostridium coccoides group, the atopobium, faecalibacterium prausnitzii and eubac ... | 2006 | 16420637 |
| differences in fecal microbiota in different european study populations in relation to age, gender, and country: a cross-sectional study. | a cross-sectional study on intestinal microbiota composition was performed on 230 healthy subjects at four european locations in france, germany, italy, and sweden. the study participants were assigned to two age groups: 20 to 50 years (mean age, 35 years; n = 85) and >60 years (mean age, 75 years; n = 145). a set of 14 group- and species-specific 16s rrna-targeted oligonucleotide probes was applied to the analysis of fecal samples by fluorescence in situ hybridization coupled with flow cytometr ... | 2006 | 16461645 |
| phylogeny of human intestinal bacteria that activate the dietary lignan secoisolariciresinol diglucoside. | the human intestinal microbiota is essential for the conversion of the dietary lignan secoisolariciresinol diglucoside (sdg) via secoisolariciresinol (seco) to the enterolignans enterodiol (ed) and enterolactone (el). however, knowledge of the species that catalyse the underlying reactions is scant. therefore, we focused our attention on the identification of intestinal bacteria involved in the conversion of sdg. strains of bacteroides distasonis, bacteroides fragilis, bacteroides ovatus and clo ... | 2006 | 16466386 |
| vaccination prevents helicobacter pylori-induced alterations of the gastric flora in mice. | molecular analysis of the gastric microflora in mice revealed that helicobacter pylori infection causes an increase in microbial diversity. the stomachs of h. pylori-infected animals were colonized by bacteria which are naturally restricted to the lower intestinal tract. clostridia, bacteroides/prevotella spp., eubacterium spp., ruminococcus spp., streptococci and escherichia coli were detected exclusively in the stomachs of infected animals, whereas lactobacilli dominated the gastric flora in n ... | 2006 | 16487303 |
| antimicrobial susceptibility of anaerobic bacteria in new zealand: 1999-2003. | routine susceptibility testing of all anaerobic organisms is not advocated, but it is useful for laboratories to test periodically for anaerobic organisms and provide local susceptibility data to guide therapy. this study reports the national trend of antibiotic susceptibility of clinically significant anaerobes in new zealand. | 2006 | 16507560 |
| eubacterium limosum ameliorates experimental colitis and metabolite of microbe attenuates colonic inflammatory action with increase of mucosal integrity. | to examine the effect of eubacterium limosum (e. limosum) on colonic epithelial cell line in vitro, and to evaluate the effect of e. limosum on experimental colitis. | 2006 | 16534848 |
| transformation of (sup14)c-lignin-labeled cell walls of wheat by syntrophococcus sucromutans, eubacterium oxidoreducens, and neocallimastix frontalis. | wheat cell walls, saponified or not, labeled with [u-(sup14)c]phenylalanine or [o-methyl-(sup14)c]sinapate were fermented by neocallimastix frontalis or syntrophococcus sucromutans plus eubacterium oxidoreducens or a mixed culture. phenolics were less solubilized but more transformed by bacteria than by the fungus, and mineralization was slight. s. sucromutans o-demethylated [o-methyl-(sup14)c]syringyl lignins, yielding labeled acetate. | 1995 | 16534916 |
| evidence for para dechlorination of polychlorobiphenyls by methanogenic bacteria. | when microorganisms eluted from upper hudson river sediment were cultured without any substrate except polychlorobiphenyl (pcb)-free hudson river sediment, methane formation was the terminal step of the anaerobic food chain. in sediments containing aroclor 1242, addition of eubacterium-inhibiting antibiotics, which should have directly inhibited fermentative bacteria and thereby should have indirectly inhibited methanogens, resulted in no dechlorination activity or methane production. however, w ... | 1995 | 16535042 |
| growth patterns of a wide spectrum of organisms encountered in clinical blood cultures using both hypertonic and isotonic media. | in an effort to determine how long a wide spectrum of organisms will survive in either an isotonic or a hypertonic blood culture system, all clinical blood culture flasks were subcultured on a daily basis for the first seven days of incubation and again on the fourteenth day. this subculture included all those flasks found to harbor organisms on previous subculture. organisms such as members of the enterobacteriaeceae and pseudomonadaceae, as well as staphylococcus aureus, bacteroides spp., euba ... | 1976 | 16535815 |
| [anaerobic bacteria isolated from patients with suspected anaerobic infections]. | the study involved 394 clinical samples sent to the clinical microbiology laboratory of hacettepe university adult hospital between january 1997 and may 2004 for anaerobic cultivation. since multiple cultures from the same clinical samples of the same patient were excluded, the study was carried on 367 samples. the anaerobic cultures were performed in anaerobic jar using anaerogen kits (oxoid, basingstoke, u.k.) or genbox (biomérieux, lyon, france). the isolates were identified by both classical ... | 2005 | 16544546 |
| analysis of the antibacterial activity and plaque control benefit of colgate total dentifrice via clinical evaluation and real-time polymerase chain reaction. | this study analyzed, from a combined clinical and molecular biologic perspective, the antibacterial and antiplaque efficacy of colgate total dentifrice (ctd). | 2005 | 16583596 |
| prevalence and temporal stability of selected clostridial groups in irritable bowel syndrome in relation to predominant faecal bacteria. | the differences in faecal bacterial population between irritable bowel syndrome (ibs) and control subjects have been reported in several studies. the aim of the present study was to compare the predominant and clostridial faecal microbiota of ibs subjects and healthy controls by applying denaturing gradient gel electrophoresis (dgge) and a recently developed multiplexed and quantitative hybridization-based technique, transcript analysis with the aid of affinity capture (trac). according to the r ... | 2006 | 16585652 |
| the mure synthetase from thermotoga maritima is endowed with an unusual d-lysine adding activity. | the peptidoglycan of thermotoga maritima, an extremely thermophilic eubacterium, was shown to contain no diaminopimelic acid and approximate amounts of both enantiomers of lysine (huber, r., langworthy, t. a., könig, h., thomm, m., woese, c. r., sleytr, u. b., and stetter, k. o. (1986) arch. microbiol. 144, 324-333). to assess the possible involvement of the mure activity in the incorporation of d-lysine, the mure gene from this organism was cloned in escherichia coli, and the corresponding prot ... | 2006 | 16595662 |