| calpain and calpastatin activity in the optic pathway. | the levels of the neutral proteolytic enzymes calpains and their endogenous inhibitor calpastatin were determined in the retina and in the retrobulbar optic pathway in the albino rabbit. the highest level of calpains was observed in the optic nerve with decreasing levels in the optic tract and superior colliculus. the level of calpastatin in the retina was very low compared to that in the optic nerve and tract and other parts of the nervous system. | 1990 | 2193255 |
| direct effect of parathyroid hormone on insulin secretion from pancreatic islets. | indirect evidence indicates that parathyroid hormone (pth) interacts with pancreatic islets and modulates their insulin secretion. this property of pth has been implicated in the genesis of impaired insulin release in chronic renal failure. we examined the direct effect of pth-(1-84) and pth-(1-34) on insulin release using in vitro static incubation and dynamic perifusion of pancreatic islets from normal rats. both moieties of the hormone stimulated in a dose-dependent manner glucose-induced ins ... | 1990 | 2193536 |
| partitioning of nucleoids. | | 1990 | 2194249 |
| serum and tissue cage fluid concentrations of ciprofloxacin after oral administration of the drug to healthy dogs. | ciprofloxacin, a fluoroquinolone antimicrobial agent, was administered orally to 4 healthy dogs at dosage of approximately 11 and 23 mg/kg of body weight, every 12 hours for 4 days, with a 4-week interval between dosing regimens. serum and tissue cage fluid (tcf) concentrations of ciprofloxacin were measured after the first and seventh dose of each dosing regimen. the peak concentration was greatest in the serum after multiple doses of 23 mg/kg (mean +/- sem; 5.68 +/- 0.54 micrograms/ml) and lea ... | 1990 | 2195927 |
| inactivation of legionella pneumophila by monochloramine. | chloramination which is used in south australia to control the growth of naegleria fowleri, was investigated to see if it would also control that of legionella pneumophila. it was found that l. pneumophila was more sensitive than escherichia coli to monochloramine. at 1.0 mg/l, a 99% kill of l. pneumophila was achieved in 15 min compared with 37 min for a 99% kill of e. coli. combined with the stability of monochloramine, even at elevated temperatures, the results suggest that this disinfectant ... | 1990 | 2196255 |
| aluminum, a neurotoxin which affects diverse metabolic reactions. | experimental evidence is summarized to support the hypothesis that chronic exposure to low levels of aluminum may lead to neurological disorders. these disorders result from defective phosphorylation--dephosphorylation reactions, reduced glucose utilization and site-specific damage inflicted by free radicals produced by altered iron metabolism. the brain is a highly compartmentalized organ. therefore, a co-localization of critical mass of metabolic errors rather than a single event may be essent ... | 1990 | 2198876 |
| [complexes of phorbol tumor promoters with protein kinase c]. | based on the literature data, a systematic comparison of relationships between the structures of incomplete and complete phorbol tumor promoters, diacylglycerols and their activities in various biological test-systems was carried out. the specific features of the phorbol esters-protein kinase c complexes responsible for the induction of the first and second stages of the tumor promotion in mouse skin, were established. the type of diacylglycerol binding to protein kinase c which confers to the l ... | 1990 | 2198948 |
| polymerase chain reaction for detection of invasive shigella flexneri in food. | the polymerase chain reaction (pcr) was used to amplify a 760-base-pair (bp) fragment with the 220-kbp invasive plasmids of enteroinvasive escherichia coli, shigella flexneri, shigella dysenteriae, shigella boydii, and shigella sonnei as templates. this pcr product was easily detected by agarose gel electrophoresis. a 210-bp acci-psti fragment lying within the amplified region was used as a probe in southern hybridization blots and showed that the pcr-generated product was derived from the invas ... | 1990 | 2200336 |
| epidemiology of shigellosis in teknaf, a coastal area of bangladesh: a 10-year survey. | the epidemiological data on shigellosis in teknaf, a coastal area of bangladesh, were reviewed for a 10-year period (1975-84). certain similarities and differences were observed in the epidemiology of the disease in teknaf when compared with urban dhaka and rural matlab. similarities included: round-the-year infection with two peaks, one in the monsoon period and the other in the winter period; high male to female attendance ratio at the treatment centre; the predominance of infection in the und ... | 1990 | 2200700 |
| molecular epidemiology of shigella infection in central australia. | shigellosis is endemic in central australia and the infections are predominantly due to shigella flexneri 6, shigella flexneri 2a and shigella sonnei. plasmid profiles of isolates collected from 1985-9, suggested that infections caused by shigella flexneri 6 were predominantly due to a single clone, whereas those caused by shigella flexneri 2a and shigella sonnei were due to several genetically diverse strains, although strains with identical plasmid profiles were found in widely separated geogr ... | 1990 | 2200701 |
| plasmid profile analysis and antimicrobial susceptibility patterns of shigella isolates from nigeria. | in an epidemiological survey, plasmid profiles and antimicrobial susceptibility testing of 100 shigella isolates in lagos, nigeria was done. all the isolates were sensitive to nalidixic acid, nitrofurantoin and ciprofloxacin. the commonest antimicrobial susceptibility pattern was resistance to ampicillin, colistin sulphate, co-trimoxazole, streptomycin and tetracycline. all but 4 of 100 isolates screened contained one or more plasmids. plasmid profile analysis distinguished more strains than did ... | 1990 | 2200702 |
| [the formulation of epidemiological hypotheses by using the methods of multivariate statistical analysis]. | the work demonstrates the main approaches to the use of the methods of multidimensional analysis for the creation of a hypothesis on the mechanism of the epidemiological process of dysentery in organized groups. the main risk factors have been established, and their role in the formation of annual, all-the-year-round and seasonal dysentery morbidity has been quantitatively evaluated. the results of analysis show the existence of diverse variants of the alimentary route of the transmission of inf ... | 1990 | 2201148 |
| [morphological changes caused by the dermonecrosis factor of the causative agents of cholera and various other intestinal infections]. | intra- or subepithelial focal purulent inflammation with necrosis of exudating leucocytes during 1-2 days is developed in consequence of intradermal injection of the living cholera vibrios, cultured on membrane agar, or their supernatants. sometimes coagulative necrosis of cover epithelium arises without preliminary purulent inflammation stage from the very beginning. intradermal injection of living cholera vibrios leads to the development of coagulative necrotic foci in derma too. the vascular ... | 1990 | 2201411 |
| childhood shigellosis: clinical and bacteriological study. | ninety-three children with shigellosis were hospitalized in a regional hospital in kuwait during the year 1988. s. flexneri was the most common isolate (54%) followed by s. sonnei (39%) and s. boydii (7%). in addition to the gastrointestinal manifestations, 14 patients (15%) developed generalized convulsions. the benign and self-limiting nature of the convulsions associated with shigellosis means that neither diagnostic procedure nor drug therapy are usually necessary. the disease was self-limit ... | 1990 | 2202839 |
| positive immunostaining of ovine congenital progressive muscular dystrophy with antibody against n- and c-terminal dystrophin. | | 1990 | 2203334 |
| mutational analysis of the virg protein, a transcriptional activator of agrobacterium tumefaciens virulence genes. | the virg protein of agrobacterium tumefaciens is required in conjunction with the vira protein for transcriptional activation of the virulence (vir) genes in response to plant phenolic compounds. these proteins are members of a family of two component regulatory systems. vir genes are activated via a cascade of phosphorylation reactions involving a specific aspartic acid residue of the virg protein. we have conducted a mutational analysis of the virg protein. by mutating conserved and nonconserv ... | 1990 | 2211523 |
| free amino acid turnover in methanogens measured by 15n nmr spectroscopy. | turnover of the nitrogen moiety from free amino acid pools in two thermophilic methanogens, methanobacterium thermautotrophicum delta h and methanococcus thermolithotrophicus sn1, has been monitored with 15n nmr spectroscopy. in cells growing exponentially on 15nh4cl, glutamate was the major soluble 15n-labeled species in both organisms. when the mb. thermoautotrophicum cells were harvested, washed, and resuspended into medium containing 14nh4cl, the resonance for [15n]glutamate decreased with a ... | 1990 | 2211697 |
| effects of thrombospondin antibody on the recovery of endothelial cells from hyperthermia. | in addition to the increased synthesis of the classical heat-shock proteins (28,000, 71,000, 73,000, 90,000 and 100,000 mr polypeptides) there is also an increase of thrombospondin in the growth medium of endothelial cells exposed to hyperthermia. the effect of a monoclonal antibody to thrombospondin on the recovery of endothelial cells from hyperthermia as it relates to cytoskeletal organization and cell spreading was assessed. the antibody interacts with the heparin-binding domain of thrombosp ... | 1990 | 2211867 |
| determination of dexamethasone in bovine tissues by coupled-column normal-phase high-performance liquid chromatography and capillary gas chromatography-mass spectrometry. | a new method for the determination of dexamethasone in bovine liver and muscle tissues has been developed. crude tissue extracts were obtained by means of a three-phase liquid-liquid extraction scheme. the resulting residue was subjected to coupled-column normal-phase high-performance liquid chromatography which served to isolate the drug for the purpose of screening and quantification. sample was injected onto the first column of the system, a phenyl column, from which a heart-cut was diverted ... | 1990 | 2211922 |
| autoantibodies to the hla-b27 sequence cross-react with the hypothetical peptide from the arthritis-associated shigella plasmid. | we previously reported elevated serum antibody levels to a peptide representing the hla-b27 polymorphic region (b27 peptide) in hla-b27(+) ankylosing spondylitis (as) patients. a plasmid (phs-2) isolated from arthritogenic shigella flexneri strains had been shown to encode an amino acid sequence homologous to hla-b27. rabbit antibody to this sequence (phs-2 peptide) strongly cross-reacted with b27 peptide and, to a much lesser extent, with klebsiella nitrogenase peptide. serum antibody levels to ... | 1990 | 2212008 |
| echocardiographic definition of the left ventricular centroid. ii. determination of the optimal centroid during systole in normal and infarcted hearts. | although two-dimensional echocardiography is widely used in both clinical and experimental evaluations of regional cardiac wall motion, there is no established clinical method for quantitative analysis of the wall motion, not even for the normal radial motion observed in short-axis images. measurement of radial wall motion requires determination of a centroid from which the radii emanate. depending on its definition, the centroid is variously affected throughout systole by cardiac translation, r ... | 1990 | 2212382 |
| the role of chemosensitive muscle receptors in cardiorespiratory regulation during exercise. | several possible mechanisms leading to the cardiorespiratory adjustments to muscular exercise can be considered. activation of the cardiovascular and respiratory centers may result from: (1) direct or reflex action of circulating metabolites (humoral control); (2) cortical influxes (central drive); (3) nervous impulses from receptors in the contracting muscles (peripheral drive). information presently available focuses most of the interest upon the muscular drive. our studies on anesthetized ani ... | 1990 | 2212481 |
| protective effect of pgd2 against ethanol-induced gastric mucosal injury in rats. | the protective effect of prostaglandin d2 (pgd2) in ethanol-, hcl-, or nacl-induced gastric mucosal injury was investigated. to clarify the mechanism, the gastric mucosal hemodynamics were also investigated using reflectance spectrophotometry and laser doppler flowmetry in anesthetized rats. pgd2 administered orally significantly reduced the ethanol-induced mucosal injury. although it did not reach any significance, pgd2 also reduced 0.6 n hcl-induced mucosal damage, and slightly reduced 25% nac ... | 1990 | 2212542 |
| anion-coupled na efflux mediated by the human red blood cell na/k pump. | the red cell na/k pump is known to continue to extrude na when both na and k are removed from the external medium. because this ouabain-sensitive flux occurs in the absence of an exchangeable cation, it is referred to as uncoupled na efflux. this flux is also known to be inhibited by 5 mm nao but to a lesser extent than that inhibitable by ouabain. uncoupled na efflux via the na/k pump therefore can be divided into a nao-sensitive and nao-insensitive component. we used dids-treated, so4-equilibr ... | 1990 | 2212979 |
| [studies on resistant mechanisms in the resistant bacteria to chlorhexidine. ii. chemical components of the cell membrane and the electron microscopical observation of cell surface structure of chlorhexidine-resistant bacteria]. | the mechanisms of resistance of serratia marcescens and pseudomonas cepacia to chlorhexidine were studied. leakage of the cellular component such as protein was observed in a chlorhexidine sensitive strain (s1) of s. marcescens when s1 was treated with chlorhexidine at 40 micrograms/ml concentration, while this phenomenon was not observed in a resistant strain (r1). the following observations were made concerning about cell surface structure in the chlorhexidine sensitive and resistant strains o ... | 1990 | 2213528 |
| new animal breeding techniques and their application. | the new biotechnologies of gene transfer, in-vitro production, cloning and sexing of embryos have been developed and are being refined with efficiencies suitable for use in animal agriculture. efficient in-vitro systems for maturing oocytes and capacitating spermatozoa, for fertilizing and developing the embryos have resulted in commercial in-vitro production of embryos. cloning of embryos by nuclear transfer has been accomplished for sheep, cattle, pigs and rabbits with nuclear material supplie ... | 1990 | 2213713 |
| plants used in guatemala for the treatment of gastrointestinal disorders. 1. screening of 84 plants against enterobacteria. | gastrointestinal disorders are important causes of morbidity in developing countries. natural healing is the traditional way of treating these diseases in guatemala. ethnobotanical surveys and literature reviews showed that 385 plants from 95 families are used in guatemala for the treatment of gastrointestinal disorders. the activity of 84 of the most commonly used plants was screened in vitro against five enterobacteria pathogenic to man (enteropathogenic escherichia coli, salmonella enteritidi ... | 1990 | 2214824 |
| a pure class ii mhc disparity does not induce rejection of cornea or heart grafts in the rat. | | 1990 | 2219343 |
| [the article by iu. p. solodovnikov the etiological structure of bacterial dysentery in the ussr]. | | 1990 | 2220214 |
| the continuous decrease in the number of sspe annual cases ten years after compulsory anti-measles immunization. | the record of sspe cases diagnosed immunochemically and serologically in our laboratory, standing for about 60% of the total new cases reported in our country, shows a significant decrease in the incidence in 1988-89 (from 5.21 new cases per year per million total population in 1987 to 1.82 cases in 1988). in 85% of the patients, sspe onset occurred at the age of 10 years or more, suggesting the possibility of a primary measles infection before anti-measles immunization became compulsory. high s ... | 1990 | 2223653 |
| the continuous decrease in the number of sspe annual cases ten years after compulsory anti-measles immunization. | the record of sspe cases diagnosed immunochemically and serologically in our laboratory, standing for about 60% of the total new cases reported in our country, shows a significant decrease in the incidence in 1988-89 (from 5.21 new cases per year per million total population in 1987 to 1.82 cases in 1988). in 85% of the patients, sspe onset occurred at the age of 10 years or more, suggesting the possibility of a primary measles infection before anti-measles immunization became compulsory. high s ... | 1990 | 2223653 |
| [detection of protease specifically splitting actin in revertants of shigella flexneri l-forms]. | proteolytic activity of cell extracts from revertants of shigella flexneri l-forms as well as biochemical properties of these strains and their sensitivity to antibiotics were studied. the protease found earlier in cells of strain e. coli a2 was shown to be synthesized by one of 8 revertants under study. this protease split actin and did not split some other proteins, its activity was inhibited by inhibitors of metalloproteases. strain 5a2c which produced the protease was similar to the strain e ... | 1990 | 2224099 |
| [cloning of shigella flexneri 2a genes coding for restriction- modification system sfl2ai]. | the sfl2ai system of restriction-modification (rm) was revealed in the cells of shigella flexneri encoded by pkmr114 plasmid belonging to the incn incompatibility group. the genes for sfl2ai rm system were cloned. the system was ascribed to the enzymes of the ecorii specificity, as shown by in vivo and in vitro experiments. restriction analysis of these genes' region and antigenic properties of the sfl2ai endonuclease pointed to significant differences between this and ecorii rm systems. | 1990 | 2227395 |
| primary structure and anticandidal activity of the major histatin from parotid secretion of the subhuman primate, macaca fascicularis. | a major macaque histatin (m-histatin 1) from the parotid secretion of the subhuman primate, macaca fascicularis, was isolated by gel filtration on bio-gel p-2 and purified to homogeneity by reversed-phase high-performance liquid chromatography on a tsk-ods c18 column. the complete amino acid sequence of m-histatin 1, determined by automated edman degradation, is: (formula; see text) m-histatin 1 contains 38 amino acid residues, a phosphoserine at residue 2, has a molecular weight of 4881.8, a ca ... | 1990 | 2229609 |
| segmental vascular resistance in postobstructive pulmonary vasculopathy. | chronic unilateral pulmonary arterial ligation has been touted as a model of arteriopathy resulting in a tremendous increase in anastomotic bronchial flow (qbr) via collaterals. to investigate its effects on the pulmonary vasculature, we ligated the left main pulmonary artery of seven dogs and 120 days later pump perfused their left lower lobes (lll) via a cannula in the pulmonary artery at pulmonary arterial flow (qpa) of 250 ml/min. we measured qbr (330 ml/min) and compared lll with control co ... | 1990 | 2246149 |
| the endocrinology of parturition in the human. | current evidence suggests that oestrogens, progesterone, relaxin, the prostaglandins, and oxytocin are all hormones concerned to a major degree with the onset and maintenance of parturition. oestrogens, relaxin, and the prostaglandins are particularly involved with cervical ripening, while prostaglandins, progesterone and oxytocin are more involved in regulating myometrial contractility. catecholamines may also have some regulatory function in relation to uterine contractions. progesterone domin ... | 1990 | 2248599 |
| mandatory hiv testing in critical care/emergency patients. | testing for the presence of the human immunodeficiency virus (hiv) remains one of the most controversial issues of this decade. among persons diagnosed to be hiv positive, social ostracism and exaggerated atypical behavior are common. the resulting impact on the delivery of healthcare services to the seropositive patient has raised many ethical and professional dilemmas. discussion of hiv testing and the subsequent effects of seropositivity on the delivery of healthcare will be emphasized. | 1990 | 2249461 |
| [bacillary dysentery--1988]. | | 1990 | 2251385 |
| [a new serotype of shigella boydii]. | two strains which belong to the same serotype of shigella were isolated from the bloody-pus stool of two patients (in 1986) and is reported in this paper. the results were identical both showing agglutination in low titer with serotype 8 of s. dysenteriae and serotype 4 of s. boydii when the two strains were checked well with all kinds of diagnostic antisera and vice versa, ie the antisera produced by the two strains were also checked well with sera prepared with the representative strains of al ... | 1990 | 2251827 |
| morphine attenuation of a conditioned emotional response in post-dependent rats. | rats placed in a test chamber where they had received repetitive shocks the previous day significantly reduced their motor activity; this was taken as indicator of a conditioned emotional response. morphine attenuated this conditioned suppression of motility, possibly due to a reduction of the anxiety associated with the expectation of the noxious stimuli. previous morphine dependence (20 mg/kg daily for 26 days) did not modify the effect of the opioid on the conditioned suppression of motility. ... | 1990 | 2253691 |
| lymphoid dendritic accessory cells of the rat. | the expression of mhc class ii antigens on potential apc is a crucial step in t-lymphocyte activation and the initiation of an immune response. the studies which are presented here were initiated to characterize the critical apc present in physiologically normal, untreated rats. such a cell should constitutively express these antigens at high density and therefore provide the apparatus necessary to provoke both primary and secondary immune responses at any time. dac were found to fulfill these c ... | 1990 | 2258188 |
| interferons and collagen production. | the immunoregulatory, antiviral, and antiproliferative agents known as the interferons have profound effects on collagen synthesis. interferons alpha, beta, and gamma suppress collagen synthesis by dermal fibroblasts. in addition, interferon gamma (ifn-gamma) inhibits the constitutively increased collagen synthesis characteristic of fibroblasts derived from lesions of patients with scleroderma. ifn-gamma also inhibits collagen synthesis by myofibroblasts and synovial fibroblast-like cells. inhib ... | 1990 | 2258640 |
| [bacteriology and epidemiology of shigellas isolated at the army hospital in djibouti]. | in a space of 26 months 108 shigellas were isolated from 104 patients with sporadic and benign diseases. 90% of the subjects were young french. both s. dysenteriae and s. boydii make up 21% of the strains studied. 13% of the germs are multiresistance. multiresistance can enhance development of serious sickness if a virulent strain comes in. | 1990 | 2263188 |
| control of choroidal blood flow by the nucleus of edinger-westphal in pigeons: a laser doppler study. | anatomical studies in birds have suggested that choroidal blood flow may be regulated by a circuit involving the following serially-connected components: the retina-the suprachiasmatic nucleus (scn)-the medial subdivision of the nucleus of edinger-westphal (mew)-the ciliary ganglion-the choroidal blood vessels. in order to better clarify the role of this circuit, we examined the effects of electrical stimulation of ew on choroidal blood flow in the ipsilateral eye, using laser doppler velocimetr ... | 1990 | 2265988 |
| construction of an auxotrophic shigella flexneri strain for use as a live vaccine. | a virulent shigella flexneri serotype y strain, sfl1, was made auxotrophic for aromatic metabolites, including p-aminobenzoic acid, which is not available in mammalian tissues, by transduction of a tn10-inactivated arod gene from escherichia coli k-12 nk5131. one transductant, sfl114, selected for further studies, had the same biochemical and serological characteristics as the parent strain and the o-antigen patterns of the two strains were identical in sds-page and western blot experiments. sfl ... | 1990 | 2266855 |
| the preferential uptake of very-low-density lipoprotein cholesteryl ester by rat liver in vivo. | the removal from the blood and the uptake by the liver of injected very-low-density lipoprotein (vldl) preparations that had been radiolabelled in their apoprotein and cholesteryl ester moieties was studied in lactating rats. radiolabelled cholesteryl ester was removed from the blood and taken up by the liver more rapidly than sucrose-radiolabelled apoprotein. near-maximum cholesteryl ester uptake by the liver occurred within 5 min of the injection of the vldl. at this time, apoprotein b uptake ... | 1990 | 2268298 |
| definition of the transcription initiation site of human plasminogen gene in liver and non hepatic cell lines. | we have mapped the cap site of the human plasminogen mrna by primer extension and pcr techniques and found that it is located at position -161 relative to the first atg, 97 bases upstream to the 5' end of the previously isolated cdna clone. seven human hepatic and non hepatic cell lines and fresh liver cells were tested for human plasminogen mrna expression: the liver and the liver derived hepg2 cell line represent the major site of plasminogen rna synthesis while the other cell lines (hep3b, he ... | 1990 | 2268308 |
| use of a spread sheet to calculate the current-density distribution produced in human and rat models by low-frequency electric fields. | the current-density distribution produced inside irregularly shaped, homogeneous human and rat models by low-frequency electric fields is obtained by a two-stage finite-difference procedure. in the first stage the model is assumed to be equipotential. laplace's equation is solved by iteration in the external region to obtain the capacitive-current densities at the model's surface elements. these values then provide the boundary conditions for the second-stage relaxation solution, which yields th ... | 1990 | 2268377 |
| functional and nmr studies of hb sassari (asp-126 alpha----his); role of the inter-subunit contacts in the affinity control of human hemoglobin. | the oxygen affinity of hemoglobin sassari (asp-126 alpha----his), a variant substituted in the alpha 1 beta 1 interface, was found to be 8-times greater relative to normal adult human hemoglobin. study of the exchangeable hydrogen-bonded protons by nmr spectroscopy shows only minor changes at the alpha 1 beta 1 interface. in particular, the resonance previously assigned to the proton of the hydrogen bond asp-126 alpha 1. . . tyr-35 beta 1 in normal hemoglobin is still present in the variant spec ... | 1990 | 2268670 |
| survey of enteropathogenic agents in children with and without diarrhoea in ghana. | a survey was carried out over 1 year in a rural area of ghana on the isolation, detection and/or identification of enteric pathogens from children under 5 years of age with and without diarrhoea. the isolation and detection rate of shigella flexneri, shigella dysenteriae, giardia lamblia and rotavirus were higher in children with diarrhoea than in controls. yersinia enterocolitica, vibrio cholerae and vibrio parahaemolyticus were not isolated during the period of this survey. the incidence of ot ... | 1990 | 2270006 |
| effects of tokishakuyakusan and keishibukuryogan on steroidogenesis by rat preovulatory follicles in vivo. | the effect of tokishakuyakusan (ts) and keishibukuryogan (kb) on estradiol-17 beta, progesterone and testosterone in serum and ovarian tissue from pms-treated immature rats was examined in vivo. the in vivo intravenous administration of 20 micrograms of ts or kb increased the concentrations of estradiol-17 beta, progesterone and testosterone. these results suggest that ts and kb stimulate in vivo the production of estradiol-17 beta, progesterone and testosterone by preovulatory follicles, and th ... | 1990 | 2270849 |
| induction of g.c to a.t transitions by the acridine half-mustard icr-191 supports a mispairing mechanism for mutagenesis by some bulky mutagens. | as the most nucleophilic atom in dna, the guanine n7 atom is a major site of attack for a large number of chemical mutagens as well as chemotherapeutic agents. paradoxically, while methylation of guanine n7 is believed to be largely nonmutagenic, aflatoxin b1, among the most potent mutagens, appears to exert its mutagenic activity through adduction at this site. on the basis of an analysis of the specificity of mutations induced by various adduct forms of aflatoxin b1, we have previously propose ... | 1990 | 2271689 |
| toxic dilatation of the colon in shigellosis. | | 1990 | 2271862 |
| shigellosis in new brunswick. | | 1990 | 2272103 |
| dna sequence analysis of the reca genes from proteus vulgaris, erwinia carotovora, shigella flexneri and escherichia coli b/r. | the complete nucleotide sequences of the reca genes from escherichia coli b/r, shigella flexneri, erwinia carotovora and proteus vulgaris were determined. the dna sequence of the coding region of the e. coli b/r gene contained a single nucleotide change compared with the e. coli k12 gene sequence whereas the s. flexneri gene differed at 7 residues. in both cases, the predicted proteins were identical in primary structure to the e. coli k12 reca protein. the dna sequences of the reca genes from e ... | 1990 | 2274037 |
| [role of protons and bicarbonate in adaptative gastric cytoprotection]. | the unknown mechanism of adaptative gastric cytoprotection (agc) induced by 20% ethanol and subsequent injury with 70% ethanol was studied in wistar rats. pretreatment with indomethacin or hgii2 did not prevent the agc, there suggesting that neither endogenous pgs nor gastric mucus take part in its mechanism. on the other hand, ranitidine pretreatment blocked and even aggravated the damage induced by ethanol-ethanol. in contrast, the latter phenomenon was reverted by 20% acidified ethanol. it is ... | 1990 | 2275313 |
| protective milk o antibodies induced in guinea pigs by parenteral shigella ribosomal vaccine. | igg and iga o antibodies were studied in milk and sera of guinea pigs subcutaneously immunized at various stages of pregnancy with shigella ribosomal vaccines (srv) from shigella sonnei and shigella flexneri. both vaccines induced o antibodies in milk, the level of iga antibodies being significantly higher than that of igg antibodies. the immune milk provided a clear-cut protection against experimental shigella-induced keratoconjunctivitis. these results are consistent with the previously shown ... | 1990 | 2276843 |
| bone allografts sterilised by irradiation. biological properties, procurement and results of 150 massive allografts. | massive bone allografts from cadavers have been sterilised by gamma-radiation from radioactive cobalt at a dose of 25,000 gray (gy). the biological effects of radiation are discussed. human cortical bone showed an acceptable 20% decrease in strength on bending tests after 27,000 gy irradiation, but higher doses are more damaging and should be avoided. the procurement protocol used at the cochin hospital is described, and the importance of dosimetry and record-keeping emphasised. the clinical res ... | 1990 | 2279829 |
| [current views on the pathomorphology and pathogenesis of dysentery]. | according to the literature and the authors' data in patients who died of dysentery shigellae are found seldom because of postmortem shedding of superficial colonic epithelium infected by them. shigella adhesion and invasion into the colonocytes are regularly found in the colon biopsies. as shown recently in experiments, shigella outer membrane proteins forming "contact haemolysin" ("virulence plasmid" product) are responsible for their invasion. in the small intestine this cytotoxin is destroye ... | 1990 | 2281980 |
| [the characteristics of the proliferative processes in the epithelium of the sigmoid intestine in acute bacillary dysentery]. | proliferative processes were studied using radioautography with 3h-thymidine at the height of the disease and in the early convalescence of the bacterial dysentery. the mucous membrane of the sigmoid colon of healthy persons undergoing rectoromanoscopy due to the epidemic reasons served as control. no pathologic changes were found in these healthy persons; the labeled nuclei index (lni) was 5.75 +/- 0.4%, the label intensity (li) characterizing the rate of dna synthesis was 14.9 +/- 0.8. during ... | 1990 | 2285363 |
| [the epidemiological characteristics of shigellosis]. | | 1990 | 2286252 |
| antigenic and catalytic disparity in the distribution of cytochrome p-450-dependent 25-hydroxyvitamin d3-1 alpha- and 24-hydroxylases. | chick 25-hydroxyvitamin d3-1 alpha-hydroxylase, a cytochrome p-450 monooxygenase with a molecular weight of 57 kda, can be isolated as described by mandel et al. (1990 b). under normal physiological circumstances, it occurs exclusively in kidney mitochondria. an isozyme of the 1 alpha-hydroxylase, known as the 24-hydroxylase, which uses the same substrate to yield an isomeric product, is also a cytochrome p-450 monooxygenase, has a molecular weight of 55 kda, and like-wise occurs in kidney mitoc ... | 1990 | 2286531 |
| [the differential diagnosis of intestinal amebiasis]. | differential diagnosis of intestinal amebiasis (106 patients), acute flexner's dysentery (105 patients) and nonspecific ulcerative colitis (31 patients) based on the clinical data and rectoromanoscopic findings is described. rapidly and gradually progressing in the first 7 days intestinal amebiasis is characterized by a gradual onset, normal t0 with mounting general weakness, celiectasia, diarrhea with hyaloid mucus, focal catarrh-hemorrhagic changes and appearance of individual oval transversal ... | 1990 | 2290391 |
| association of thrombotic thrombocytopenic purpura and human immunodeficiency virus infection. | a 35 year-old hiv-positive male intravenous drug abuser developed thrombotic thrombocytopenic purpura (ttp) during the course of recto-sigmoiditis secondary to shigella flexneri. complete remission was achieved by aggressive treatment of a combination of plasma exchange, fresh frozen plasma infusion, continuous prostacyclin perfusion, p.o. administered aspirin-dipyridamol and intravenous injections of vincristine. during acute microangiopathy, an acquired type ii von willebrand disease was diagn ... | 1990 | 2290713 |
| intracellular lytic enzyme systems and their use for disruption of escherichia coli. | this article focusses on lytic enzyme systems available in e. coli and their potential use for cellular disruption. in the systems described here the genetic information for lysis would be carried within the microbial host, either integrated or naturally occurring on chromosomal dna, or on extrachromosomal elements such as plasmids. each microbe would carry complete information for endogenous enzymatic lysis, and lysis would occur in a controlled manner after being triggered by an external facto ... | 1990 | 2291440 |
| changes in the antibiotic sensitivities of urinary pathogens, 1971-1989. | all urinary pathogens from general practice and hospital have been tested for sensitivity to a range of antimicrobial agents for the last 19 years. there have been marked changes during that time. in general practice there has been a considerable increase in the proportion of staphylococcal infections from 5.1% to a peak of 14.8% in 1982 and a more recent decline to 3.4%. there has also been a decrease in the proportion caused by proteus mirabilis, from 9.2% to 5.0%. similar, but smaller changes ... | 1990 | 2292543 |
| production of propeptide-directed antibody: its application to the purification of proalbumin and analysis of proalbumin processing. | antibodies which specifically recognize rat proalbumin, but not mature serum-albumin, were raised. propeptide (nh2-arg-gly-val-phe-arg-arg) with an additional cysteine residue at the carboxyl terminus was conjugated to ovalbumin through either sulfide or amino group and the conjugates were injected into rabbits. monospecific antibodies were purified on a propeptide-coupled affinity column and further immobilized as an affinity support, allowing us to purify proalbumin in a single step from rat l ... | 1990 | 2292582 |
| a sensitive in vitro functional assay to detect k(+)-channel-dependent vasodilators. | this study describes a sensitive in vitro relaxation assay using isolated rabbit mesenteric artery to detect the activity of a vasodilator as a k(+)-channel activator. thus, comparison of several known k(+)-channel activators was made with other vasodilators known to work via various cellular mechanisms. the vasodilators used were minoxidil sulfate (mnxs; 5 microm), brl-34915 (cromakalim, 0.1 microm), nicorandil (10 microm), pinacidil (1 microm), diazoxide (100 microm), sodium nitroprusside (10 ... | 1990 | 2292878 |
| evaluation of combined effects in dose-response studies by statistical comparison with additive and independent interactions. | an improved method for the evaluation of combined drug effects by means of dose-response curves (drcs) is described. a drug, a, was tested in the absence and presence of a fixed concentration of another drug, b, mainly in organ-bath experiments with smooth muscle strips from bovine coronary arteries and tracheal muscle. the results of such experiments are expressed in terms of percent of maximum response. median values, rather than mean values, for each concentration of drug a were determined in ... | 1990 | 2292882 |
| in vitro skin absorption and metabolism of benzoic acid, p-aminobenzoic acid, and benzocaine in the hairless guinea pig. | the percutaneous absorption and metabolism of three structurally related compounds, benzoic acid, p-aminobenzoic acid (paba), and ethyl aminobenzoate (benzocaine), were determined in vitro through hairless guinea pig skin. benzocaine was also studied in human skin. absorption of benzocaine was rapid and similar through both viable and nonviable skin. the absorption of the two acidic compounds, benzoic acid and paba, was greater through nonviable skin. a small portion (6.9%) of absorbed benzoic a ... | 1990 | 2293213 |
| release of human serum albumin from poly(lactide-co-glycolide) microspheres. | human serum albumin (hsa) was encapsulated in a 50:50 copolymer of dl-lactide/glycolide in the form of microspheres. these microspheres were used as a model formulation to study the feasibility of controlling the release of large proteins over a 20- to 30-day period. we show that hsa can be successfully incorporated into microspheres and released intact from these microspheres into various buffer systems at 37 degrees c. a continuous release of the protein could be achieved in physiological buff ... | 1990 | 2293220 |
| production of specific monoclonal antibodies to salmonella typhi flagellin and possible application to immunodiagnosis of typhoid fever. | four murine monoclonal antibodies (mabs) to salmonella typhi flagellin were produced. these mabs did not react with eight other enterobacterial strains tested: salmonella enteritidis, salmonella typhimurium, salmonella paratyphi a, escherichia coli, shigella flexneri, shigella sonnei, yersinia enterocolitica, and campylobacter jejuni. all four mabs cross-reacted with salmonella muenchen flagellin indicating specificity for d antigenic flagellar epitope. one mab (c4) was selected to develop a dou ... | 1990 | 2295860 |
| investigation by amperometric methods of intracellular reduction of 2,6-dichlorophenolindophenol in normal and transformed hepatocytes in the presence of different inhibitors of cellular metabolism. | the reduction of 2,6-dichlorophenolindophenol (dcip) was measured by amperometric methods in morris hepatoma 3924a cells, normal isolated rat hepatocytes and in mitochondria isolated from normal rat liver. the influence of aerobic and anaerobic atmospheres and of various inhibitors of cellular metabolism, especially of the respiratory chain (kcn, nan3, oligomycin), on dcip-reduction were studied using glucose, succinate, beta-hydroxybutyrate, alpha-keto-glutarate and oxalacetate as substrates. u ... | 1990 | 2297512 |
| virg, an agrobacterium tumefaciens transcriptional activator, initiates translation at a uug codon and is a sequence-specific dna-binding protein. | the agrobacterium tumefaciens ti plasmid virg locus, in conjunction with vira and acetosyringone, activates transcription of the virulence (vir) genes. insertional and deoxyoligonucleotide-directed mutagenesis studies showed that both octopine and nopaline ti plasmid virg genes initiate translation at a uug codon. virg protein initiated at this uug codon was found to be 241 amino acid residues in length and had an apparent molecular mass of 27.1 kilodaltons. a salmonella typhimurium trp-virg tra ... | 1990 | 2307647 |
| usefulness of rapid production model for pancreatic carcinoma in male hamsters. | the rapid production model of pancreatic duct adenocarcinoma established previously in female syrian hamsters was applied to male hamsters to confirm the efficacy of the "augmentation pressure" procedure and to reveal sex difference in response to this procedure. hamsters received 70 mg/kg n-nitrosobis (2-oxopropyl) amine (bop) as an initiation and two cycles of "augmentation pressure" consisting of ethionine on a choline-deficient diet, methionine and bop and an additional 20 mg/kg bop and were ... | 1990 | 2317782 |
| ca2+ transients are not required as signals for long-term neurite outgrowth from cultured sympathetic neurons. | a method for clamping cytosolic free ca2+ ([ca2+]i) in cultures of rat sympathetic neurons at or below resting levels for several days was devised to determine whether ca2+ signals are required for neurite outgrowth from neurons that depend on nerve growth factor (ngf) for their growth and survival. to control [ca2+]i, normal ca2+ influx was eliminated by titration of extracellular ca2+ with egta and reinstated through voltage-sensitive ca2+ channels. the rate of neurite outgrowth and the number ... | 1990 | 2324199 |
| tibenelast, 5,6-diethoxybenzo(b)thiophene-2-carboxylic acid, sodium salt (ly186655), is an orally active anti-asthma compound in the guinea pig. | anaphylactic shock was induced in actively sensitized guinea pigs by free inhalation of a high dose of ovalbumin (10 mg/ml) aerosol. tibenelast (ly186655), 5,6-diethoxybenzo(b)-thiophene-2-carboxylic acid, sodium salt, proved to be a potent orally active compound against anaphylactic shock induced by high dose antigen aerosol. when a lower aerosol challenge (0.05 mg/ml) was employed, bronchoconstriction was observed with a concomitant increase in lung resistance (rl) and a fall in dynamic compli ... | 1990 | 2329918 |
| therapeutic value of gramurin (oxolinic acid) in shigella infections and shigella-negative dysentery syndrome. | a total of 10 shigella-positive and 10 shigella-negative dysentery syndrome patients were treated with daily 2-g gramurin doses for 3 days. favourable clinical results could be obtained in response to the therapy. the complaints of the patients ceased within 48 hours. bacterial negativity was recorded on days 3, 4, and 5 after cessation of therapy in shigella flexneri-positive cases (5 cases), while in case of shigella sonnei positivity bacterium negativity could be obtained within the abovement ... | 1990 | 2345905 |
| epidemiology of bacterial pathogens associated with infectious diarrhea in djibouti. | during a survey examining the causes of diarrhea in the east african country of djibouti, 140 bacterial pathogens were recovered from 209 diarrheal and 100 control stools. the following pathogens were isolated at comparable frequencies from both diarrheal and control stools: enteroadherent escherichia coli (eaec) (10.6 versus 13%), enterotoxigenic e. coli (etec) (11 versus 10%), enteropathogenic e. coli (epec) (7.7 versus 12%), salmonella spp. (2.9 versus 3%), and campylobacter jejuni-c. coli (3 ... | 1990 | 2351738 |
| role of bacterial and host factors in the pathogenesis of shigella septicemia. | to determine the role of bacterial and host factors in the pathogenesis of shigellemia, blood and fecal shigella isolates were compared for serum resistance and siderophore production, and shigellemic patients were examined for decreased serum bactericidal activity or increased serum transferrin saturation compared to control patients with non-bacteremic shigellosis. the majority of both blood (36/38) and fecal (36/48) shigella isolates were sensitive to normal serum (greater than 2 log kill/60 ... | 1990 | 2373088 |
| alterations of cellular characteristics of a human ovarian teratocarcinoma cell line after in vitro treatment with retinoids. | differentiation of the human teratocarcinoma derived cell line. pa-1, with retinoids was examined at concentrations (10(-6)-10(-8) m) that did not exhibit an antiproliferative effect during log-phase growth. treatment with naturally occurring retinoic acid or certain synthetic retinoids (13-cis retinoic acid, ro10-9359, and ro13-7410), while not significantly altering the log-phase growth rate, decreased the saturation cell density and mitotic indices after confluence. retinoid treatment also in ... | 1990 | 2373285 |
| effect of neuromuscular blockade on fetal oxidative metabolism. | because fetal neuromuscular blockade has been used during cord blood sampling procedures to prevent fetal movement, we studied the effect of pancuronium bromide on fetal arterial concentrations of glucose, lactate, and oxygen and their umbilical uptake in 6 chronically prepared fetal sheep. we observed a significant increase in the arterial concentrations and decrease in the umbilical uptakes. we suggest that cord blood sampling procedures for fetal assessment of oxidative metabolism not be perf ... | 1990 | 2390540 |
| morphologic characterization of osteoblast-like cell cultures isolated from newborn rat calvaria. | two methods for harvesting osteoblast-like cell populations from newborn (10 days) rat calvaria were compared. the first one consisted in culturing the periosteum-free bones and then trypsinizing the cells on the bone surface. the second one involved the migration of the osteoblasts on glass fragments before trypsinization. since the plating efficiency, the proportion of alkaline phosphatase-positive cells, the population doubling time, and the calcium deposition were more adequate, the second m ... | 1990 | 2390733 |
| phosphorylation of the virg protein of agrobacterium tumefaciens by the autophosphorylated vira protein: essential role in biological activity of virg. | agrobacterium tumefaciens virulence genes are induced by plant signals through the vira-virg two-component regulatory system. the vira protein is a membrane-spanning sensor molecule that possesses an autophosphorylating activity, and the virg protein is a sequence-specific dna-binding protein. in this report, we demonstrate that the virg protein is phosphorylated by the vira protein and that the phosphate is directly transferred from the phosphorylated vira molecule (phosphohistidine) to the vir ... | 1990 | 2394678 |
| tertiary structure of oxidized flavodoxin from an eukaryotic red alga chondrus crispus at 2.35-a resolution. localization of charged residues and implication for interaction with electron transfer partners. | the crystal structure of the oxidized form of a flavodoxin from an eukaryotic red alga, chondrus crispus, has been determined by multiple isomorphous replacement and anomalous scattering methods. a model of the 173 residues and flavin mononucleotide (fmn) has been refined by a restrained least squares method to a crystallographic r-factor of 22.6% using 6236 reflections between 6.0 and 2.35 a with f greater than 3 sigma f. this molecule has a sheet consisting of five parallel beta-strands with t ... | 1990 | 2394748 |
| onset of the hormone-sensitive perinatal period for sexual differentiation of the sexually dimorphic nucleus of the preoptic area in female rats. | the volume of the sexually dimorphic nucleus of the preoptic area (sdn-poa) of the rat brain is severalfold larger in males than in females. the volume of the sdn-poa can be influenced significantly by the hormonal milieu during the perinatal "critical period" of sexual differentiation of the brain. the purpose of the present study was to determine the onset of this period of sexual differentiation of the sdn-poa. pregnant rats received no treatment or were injected subcutaneously with oil on da ... | 1990 | 2394991 |
| effects of castration and testosterone treatment on the activity of testosterone-metabolizing enzymes in the brain of male and female zebra finches. | recently, we described the distribution of testosterone-metabolizing enzymes (i.e., aromatase, 5 alpha- and 5 beta-reductases) in the zebra finch (taeniopygia guttata) brain using a sensitive radioenzyme assay combined to the palkovits punch method. a number of sex-differences in the activity of these enzymes were observed especially in nuclei of the song-control system. the hormonal controls of these differences have now been analyzed by gonadectomizing birds of both sexes and by giving them a ... | 1990 | 2394994 |
| effect of glycerol on the hemodynamics of acutely induced ischemic area in the cerebral cortex of cats. | the effect of glycerol on the hemodynamics of ischemic areas of the brain was examined. employing a photoelectric apparatus, the cbv (vol%), mean transit time of blood(s), and cbf (ml/100 g/min) in the cortex of the left ectosylvian gyrus were determined in 16 anesthetized cats before, at 1 hr, and at 2 hr after left mca occlusion. in 10 cats, 10% glycerol in 5% fructose saline was intravenously administered at a speed of 5 ml/kg/hr at 1 hr after mca occlusion. the other animals served as the co ... | 1990 | 2396522 |
| doxycycline prophylaxis for shigellosis. | the efficacy of oral doxycycline, 100 mg/d for 14 days, in reducing the incidence of shigellosis in newcomers to an area hyperendemic for the disease was examined in a double-blind, placebo-controlled trial. of 107 entrants, 100 completed the study; 50 received the drug and 50 received a placebo. diarrheal disease and associated symptoms were monitored for 8 weeks. starting on the 3rd day of the trial, an outbreak was observed, and shigella flexneri type 2a was isolated from 6 subjects. eight of ... | 1990 | 2404480 |
| contribution of superoxide dismutase and catalase activities to shigella flexneri pathogenesis. | a shigella flexneri serotype 5 strain deficient in the production of the iron-containing superoxide dismutase fesod (sodb) and a catalase-negative (katfg) s. flexneri serotype 5 strain were isolated. both strains were examined for increased sensitivity to oxygen stress by using assays involving killing by mouse peritoneal macrophages and human polymorphonuclear leukocytes as well as infection of rabbit ileal loops. the sodb mutant was extremely sensitive to killing by phagocytes when compared wi ... | 1990 | 2404874 |
| acute glomerulonephritis due to shigella flexneri dysentery with demonstration of a virulence protein of shigella in circulating immune complexes. | | 1990 | 2405072 |
| [theoretical conformation analysis of tetrasaccharide-repeated links of the shigella flexneri o-antigenic polysaccharide]. | theoretical conformational analysis of four tetrasaccharide repeating units of the shigella flexneri serogroup y polysaccharide has been carried out. interdependency of conformational states of neighbouring disaccharide units in the oligosaccharides has been investigated and conformational distribution of tetrasaccharides in solution calculated. taking into account the entropy of oligosaccharide chains is shown to lead to significant correction of the results. | 1985 | 2408628 |
| a heat-modifiable outer membrane protein carries an antigen specific for the species yersinia enterocolitica and yersinia pseudotuberculosis. | the outer membranes of gram-negative bacteria are considered to be of importance in host-bacteria interaction, in protective immunity, and occasionally in subclassification within a species. in this study, the outer membranes of several strains of yersinia enterocolitica and y. pseudotuberculosis were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). it was found that the appearance of the major proteins depended on the temperature at which they were solubilized i ... | 1985 | 2409152 |
| [synthesis of oligosaccharide fragments of o-specific polysaccharides of shigella flexneri. iii. synthesis of the tetrasaccharide glc alpha 1-3rha alpha 1-2(glc alpha 1-3)rha alpha 1-ome and the pentasaccharide glcnac beta 1-2(glc alpha 1-3)rha alpha 1-2(glc alpha 1-3)rha alpha 1-ome]. | two synthetic schemes to prepare the title branched tetrasaccharide and pentasaccharide are described. these oligosaccharides represent fragments of the o-antigenic polysaccharides of shigella flexneri serotype 5b. | 1985 | 2412567 |
| autoaggressive t lymphocyte lines recognizing the encephalitogenic region of myelin basic protein: in vitro selection from unprimed rat t lymphocyte populations. | autoimmune t lymphocyte lines have been established from unprimed normal rat lymph node cell populations. in a first, negative-selection round, spontaneously proliferating (smlr) t cells were eliminated by a pulse of budr followed by short wave light irradiation. in a second, positive-selection round, the smlr-depleted populations were confronted with mbp presented by syngeneic spleen adherent cells. reactive t cells were propagated until stable, permanent t lines were established. all lines wer ... | 1985 | 2413113 |
| [membrane activity of a cell-free filtrate of shigella flexneri 2a]. | in the course of metabolism in shigella flexneri 2a strain there was noted secretion of a membrane-tropic factor into media. this factor increased the conductivity of bilayer lipid membranes causing formation of ionic channels in them. minimal conductivity of the channels was about 25 ps in 0.5 m nacl; at ph 7-8 the channels exhibited cation-anion selectivity. the calculated number of cation transfer (t+) was elevated with an increase in the channel amplitude, approaching 0.86. since the conduct ... | 1985 | 2413620 |
| [use of immunoenzyme analysis for evaluating the specificity of the antibody recognition of the antigenic determinants of streptococcus group a]. | investigations carried out with the use of a celia system have revealed that antibodies in the sera of patients with primary erysipelas and antibodies in rabbit antiserum to the ribosomes of group a streptococcus specifically bind with adsorbed streptococcal ribosomes, recognizing the antigenic determinants of streptococcal ribosomes, which differ from those of individual gram-negative prokaryotes (escherichia coli, shigella sonnei, shigella flexneri, salmonella minnesota). the modified celia sy ... | 1985 | 2413662 |
| [immune response and immune tolerance to the o-antigen of shigella flexneri vi]. | the method for the determination of the number of cells synthetizing antibodies to s. flexneri vi o-antigen in the spleen of mice has been developed. primary immune response to this antigen has been studied with the use of the new method. immune response to the optimum immunogenic dose of o-antigen has a manifest variable character. the intensity of primary immune response has been shown to rise with the increase of the dose of o-antigen from 0.004 to 50 micrograms. the preliminary injection of ... | 1985 | 2413664 |
| genetic determinants of virulence in shigella and dysenteric strains of escherichia coli: their involvement in the pathogenesis of dysentery. | | 1985 | 2414072 |
| [specific antigens of the causative agents and their antibodies in the circulating immune complexes in acute dysentery]. | the level of circulating immune complexes has been determined in 53 patients in the dynamics of the disease. for the first time circulating immune complexes have been found to contain shigella sonnei k-antigen and shigella flexneri o-antigen, as well as iga, igg and igm to shigella. shigella antigens can be detected from the first week of the disease, and their occurrence does not depend on the level of circulating complexes in patients blood serum. | 1985 | 2414952 |