| phenotypic characterization of quinolone-resistant mutants of enterobacteriaceae selected from wild type, gyra type and multiply-resistant (mara) type strains. | the nctc type strains of escherichia coli, enterobacter cloacae, serratia marcescens and klebsiella pneumoniae were exposed to 3, 5 and 10 x mic of nalidixic acid, enoxacin, ciprofloxacin, pd 117596 and pd 127391. from each strain a mutant with a high mic of quinolones alone (gyra) and a mutant with intermediate resistance to quinolones, some beta-lactams, chloramphenicol and tetracycline (multiply resistant, m-r) were selected on agar containing antibiotics. the gyra mutants required a higher c ... | 1991 | 1663926 |
| passive immune protection from diarrhea caused by rotavirus or e. coli: an animal model to demonstrate and quantitate efficacy. | | 1991 | 1667071 |
| in vivo evaluation of nm441, a new thiazeto-quinoline derivative. | nm441 is a lipophilic prodrug of a new thiazeto-quinoline carboxylic acid derivative nm394, and when it is administered orally it is readily absorbed and hydrolyzed to its parent compound. after oral administration of nm441 at a dose of 20 mg/kg to dogs, the peak concentration of nm394 in plasma was 2.39 micrograms/ml, whereas it was 0.63 micrograms/ml for nm394 administered alone. the in vivo activity of nm441 was compared with those of ciprofloxacin, ofloxacin, and enoxacin in mouse protection ... | 1991 | 1667252 |
| epidemiological study of an outbreak of serratia marcescens in a haemodialysis unit. | twenty-three isolates of serratia marcescens were isolated over a 10-month period from the blood and arteriovenous shunt sites of patients undergoing haemodialysis in an artificial kidney unit. surveillance measures performed on the equipment, sterile materials, environment and personnel of this unit yielded serratia from the air conditioner and one of the dialysis units. the isolates from the patients and dialyser unit were pigmented and had an identical biochemical profile, antibiograms, phage ... | 1991 | 1679073 |
| serratia marcescens bacteremia. | serratia marcescens bacteremia has become ubiquitous recently. s. marcescens bacteremia, either hospital- or community-acquired, can no longer be treated as insignificant. we reviewed 23 episodes of s. marcescens bacteremia in 1985. among them, 17 patients (74%) were hospital-acquired infections, while 6 (26%) were community-acquired. nine patients died, and the case fatality rate was 39%. eleven patients (48%) had no clinically apparent source of infection, 5 (22%) had urinary tract infection, ... | 1991 | 1679115 |
| abnormal isoform of prion protein accumulates in follicular dendritic cells in mice with creutzfeldt-jakob disease. | we established that follicular dendritic cells (fdcs) are the site of abnormal prion protein (prpcjd) accumulations in lymphoid tissues from mice infected with creutzfeldt-jakob disease. evidence of positive fdc staining was observed in creutzfeldt-jakob disease-infected mice irrespective of the inoculation route, while no such staining was seen in the control mice. we also found that the severe combined immunodeficiency mouse trait is transmittable via the intracranial route but not via the int ... | 1991 | 1681118 |
| role of superoxide in renal scarring following infection by mannose-sensitive piliated bacteria. | the role of superoxide in scar formation following renal infection caused by mannose-sensitive (ms) piliated strains of bacteria was studied in the experimental pyelonephritis model using female sprague-dawley rats. the ms piliated strain stimulated renal scarring to a significantly greater extent than either the non-piliated or mr-piliated strain. modulation of leukocytes by administering cyclophosphamide to induce neutropenia and colchicine to inhibit leukocyte migration was effective in preve ... | 1991 | 1681608 |
| cd4+ t cells regulate the magnitude of the antibody response to several helper t cell independent antigens. | the effect of prior depletion of cd4+ t cells on the magnitude of the primary antibody response to several antigens considered to be helper t cell independent (ti) was examined. treatment of mice with the monoclonal antibody gk1.5 to remove cd4+ t cells had little effect on the magnitude of the specific antibody response to the lipopolysaccharide (lps) of escherichia coli 0113; however, such treatment reduced the magnitude of the splenic igm antibody response to e. coli 055 lps, serratia marcesc ... | 1991 | 1682242 |
| antimicrobial activities of piperacillin alone and in combination with tazobactam against beta-lactamase-producing bacteria. | tazobactam (ytr 830), a new beta-lactamase inhibitor, was evaluated for its effect in combination with piperacillin, a broad spectrum, but beta-lactamase sensitive, penicillin, against 14 common bacteria. a total of 1,086 clinical isolates from different clinical specimens were tested for beta-lactamase production by the rapid chromogenic cephalosporin method. their susceptibilities to piperacillin alone and in combination with tazobactam in a ratio of 8:1 by the agar dilution method were evalua ... | 1991 | 1685175 |
| the rna antiterminator causes transcription pausing in the leader region of the tryptophan operon. | in vitro transcription studies with a trp leader dna template derived from a double deletion mutant of serratia marcescens revealed that the transcription complex pauses synthesis of part of the rna antiterminator, structure 2:3. pausing was enhanced by nusa protein and was dependent on the concentration of utp in the transcription reaction mixture. a weak antiterminator pause also was detected during transcription of the wild-type s. marcescens trp leader template in the presence of nusa protei ... | 1990 | 1690721 |
| inactivation of chemotactic activity of c5a by the serratial 56-kilodalton protease. | the effects of the 56-kilodalton protease (56k protease) from serratia marcescens on complement-derived chemotactic activity were examined. fresh human serum was incubated with zymosan to produce c5a. this activated serum was then incubated with various concentrations of 56k protease, and the chemotactic activity of mouse peritoneal exudate polymorphonuclear leukocytes (pmn) and macrophages was evaluated. a significant dose-dependent decrease of chemotactic activity was observed after protease t ... | 1990 | 1691142 |
| deoxyribonuclease zymography adapted to the precast phastgel electrophoresis media. | a set-up for casting fluorescent indicator agarose gels on ultrathin polyacrylamide microelectrophoresis gel media (pharmacia phastgel media) is described. the zymogram system allows a rapid and sensitive detection of deoxyribonuclease in various gel media, following isoelectric focusing, native and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. | 1990 | 1692791 |
| structure of the putative o23 antigen of serratia marcescens. | a neutral glycan containing l-rhamnose and 2-acetamido-2-deoxy-d-galactose is one of two polymers present in the lipopolysaccharide extract from the reference strain for serratia marcescens serogroup o23. the glycan, which has the disaccharide repeating-unit shown, shares structural features with polymers from several other o serogroups. ----4)-alpha-l-rhap-(1----4)-beta-d-galpnac-(1----. | 1990 | 1693310 |
| serratia marcescens rpr gene sensitizes escherichia coli wild-type, xth, and nfo strains to methyl methanesulphonate. | it is reported here that the rpr dna repair gene of serratia marcescens does not complement an escherichia coli xth nfo ap endonuclease mutation for resistance to methyl methanesulphonate (mms). rather, rpr sensitized escherichia coli wild-type, xth, and nfo strains to mms. also, it was found that rpr could not complement a triple tag alka reca mutation in e. coli, indicating that there are limits to rpr complementing capabilities. it was determined that rpr gene dosage was not a factor in reca ... | 1990 | 1693747 |
| identification of a rabies virus t cell epitope on the basis of its similarity with a hepatitis b surface antigen peptide presented to t cells by the same mhc molecule (hla-dpw4). | the antigenic determinant recognized by a hla-dpw4-restricted human t cell clone specific for rabies virus was identified by using a vaccinia-rabies nonstructural phosphoprotein recombinant virus and synthetic peptides of the sequence of rabies nonstructural ag. these peptides were selected on the basis of three models that predict t cell epitopes. the antigenic determinant recognized by the rabies virus-specific t cell clone contained a five-amino acid segment highly homologous to a sequence fo ... | 1990 | 1694205 |
| cloning, partial sequence, expression, and antigenic analysis of the filamentous hemagglutinin gene of bordetella pertussis. | the gene coding for the filamentous hemagglutinin (fha), one of the main factors involved in mediating adherence of bordetella pertussis to ciliated host cells, was cloned in escherichia coli, and the 3,500-base-pair nucleotide sequence encoding the amino-terminal region was determined. molecular cloning, together with the characterization of recombinant fha-related proteins produced in e. coli, revealed that the primary translation product is a protein of about 370 kilodaltons (kda). the mature ... | 1990 | 1696934 |
| monoclonal antibodies detecting discrete epitopes of human perforin. | perforin is a cytolytic protein of natural killer (nk) cells and cytotoxic t cells (ctl). purified perforin has been shown to cause cell lysis and to form stable pores in the target cell membrane, but its relevance to cytolysis in vivo is not clear. the gene for human perforin has been cloned, but monoclonal antibodies (mabs) have not been available. in order to study further its role in cytotoxicity, we have generated mabs to different regions of human perforin. four mabs were produced from mic ... | 1990 | 1698318 |
| monoclonal antibodies to quebec strain (q17) of bovine rotavirus. | fourteen monoclonal antibodies (mabs) against "quebec" strain (q17) of bovine rotavirus were isolated and characterized. four were specific for viral protein vp7 and ten were specific for viral protein vp6. five different isotypes were represented by this group of antibodies. all of the anti-vp6 and none of the anti-vp7 antibodies were sensitive to the effects of periodate on their antigen. the antibodies could be separated into three groups based on their relative resistance to the dissociation ... | 1990 | 1698323 |
| the regulation and expression of mhc class i genes. | the expression of mouse mhc class i genes and their products in vivo reveals complex patterns of regulation. different promoter elements, which are required for gene activation or modulation in response to various external stimuli, have now been characterized as well as the proteins that bind to them. as described here by brigitte david-watine and colleagues, the picture that has gradually emerged from these in vitro studies is of an intricate interplay of transacting factors that ultimately lea ... | 1990 | 1698378 |
| projection subtypes of rat neostriatal matrix cells revealed by intracellular injection of biocytin. | individual neostriatal-matrix spiny neurons were stained intracellularly with biocytin after intracellular recording in vivo, and their axons were traced into the globus pallidus (gp), entopeduncular nucleus (ep), and/or substantia nigra (sn). the locations of the neurons within the matrix compartment of the neostriatum (ns) were established by immunocytochemical counterstaining of sections containing the cell bodies using antibodies for calbindin-d28k. this allowed nearly complete visualization ... | 1990 | 1698947 |
| [o-serotypes, biochemical properties and antimicrobial susceptibilities of serratia marcescens isolated from clinical specimens (2nd report)]. | o-serotypes, biochemical properties and drug resistances of serratia marcescens (129 strains) isolated from clinical specimens in showa university fujigaoka hospital from april 1985 to march 1987, were compared with those of the previous results (jpn j clin pathol 35:555-560, 1987). the isolation frequencies of serotype o 3 and o 23 strains were 35.7% and 8.5%, respectively. these strains with serotype o 3 or o 23 were wide-spread in the hospital. the urea-positive strains which occupied 61.2% o ... | 1990 | 1700174 |
| infection of an implantable cardioverter defibrillator: management without removal of the device in selected cases. | a case is presented in which an implantable cardioverter defibrillator (icd) became infected in the abdominal wall pocket 5 weeks following implantation. there was no evidence clinically or by computed tomographic scan suggesting mediastinal extension of the infection. the infection was treated successfully by debriding the abdominal wall pocket followed by a combination of pocket irrigation with antibiotic solution, parenteral antibiotics, and long-term oral antibiotics. this conservative thera ... | 1990 | 1701884 |
| protective effect of recombinant human granulocyte colony-stimulating factor (rg-csf) against various microbial infections in neutropenic mice. | protective effect of recombinant human granulocyte colony-stimulating factor (rg-csf) on microbial infections was studied in cyclophosphamide (cpa)-induced neutropenic mice. the neutropenic mice showed severely decreased resistance against systemic infections of pseudomonas aeruginosa, escherichia coli, serratia marcescens, staphylococcus aureus, and candida albicans. when such mice were injected subcutaneously with rg-csf on four consecutive days beginning the day after cpa injection, the decre ... | 1990 | 1704477 |
| complement-mediated lysis of trypanosoma cruzi trypomastigotes by human anti-alpha-galactosyl antibodies. | antibodies that lyse trypomastigotes in a complement-mediated reaction are believed to be the main participants in the protection against virulent trypanosoma cruzi. antibodies with a specificity for alpha-galactosyl-containing determinants--generally called antigal--were studied to determine their role in the lysis of trypomastigote forms. the titers of antigal markedly increase in chagas's disease. in the present study we demonstrate binding of this antibody to t. cruzi and the complement-medi ... | 1991 | 1706399 |
| structure of a glucorhamnan from the lipopolysaccharide of serratia marcescens strain s1254. | | 1991 | 1709820 |
| interspecies compatibility of selenoprotein biosynthesis in enterobacteriaceae. | several species of enterobacteriaceae were investigated for their ability to synthesize selenium-containing macromolecules. seleniated trna species as well as seleniated polypeptides were formed by all organisms tested. two selenopolypeptides could be identified in most of the organisms which correspond to the 80 kda and 110 kda subunits of the anaerobically induced formate dehydrogenase isoenzymes of e. coli. in those organisms possessing both isoenzymes, their synthesis was induced in a mutual ... | 1991 | 1710885 |
| serological relationships of the o antigens of klebsiella pneumoniae o5, escherichia coli o8 and a new o serotype of serratia marcescens. | klebsiella pneumoniae o5, escherichia coli o8 and serratia marcescens 3255 were shown to cross-react in both elisa and immunoblotting. the cross-reaction appeared to be due to the o antigen of their lipopolysaccharide (lps). in addition, there was evidence that the reactions of these strains with their homologous antisera were due, in part, to determinants other than o polysaccharide. | 1991 | 1713181 |
| structure and function of a bacterial mrna stabilizer: analysis of the 5' untranslated region of ompa mrna. | the 5' untranslated region (utr) of the escherichia coli ompa transcript functions in vivo as a growth rate-regulated mrna stabilizer. the secondary structure of this mrna segment has been determined by a combination of three methods: phylogenetic analysis, in vitro probing with a structure-specific rnase, and methylation by dimethylsulfate in vivo and in vitro. these studies reveal that despite extensive sequence differences, the 5' utrs of the ompa transcripts of e. coli, serratia marcescens, ... | 1991 | 1713205 |
| [diagnosis and therapy of viral hepatitis]. | | 1991 | 1713243 |
| comparative biotyping, bacteriocin typing, and serogrouping (o-antigens) of serratia liquefaciens. | a total of 83 clinical isolates of serratia liquefaciens from 81 patients were biotyped, bacteriocin typed (with group a phage tail bacteriocins from s. marcescens), and serogrouped. biotyping afforded least discrimination, because 71 of the 83 isolates (85.5%) comprised 2 biotypes; the remainder were of 5 different biotypes. bacteriocin typing differentiated 70 of the 83 isolates (84.3%) into 20 types. polyclonal rabbit anti-o immune sera identified 16 o-antigens, and all 83 isolates could be s ... | 1991 | 1718303 |
| serotyping of serratia liquefaciens: h-antigens. | polyclonal anti-h rabbit immune sera differentiated 12 flagellar (h) antigens among 79 motile of 83 clinical isolates of serratia liquefaciens from 81 patients. seven of the anti-h s. liquefaciens sera cross-reacted with h-antigens of s. marcescens, specifically anti-s. liquefaciens h6, h7, h8, h9, h10, h11, and h12 with s. marcescens h-antigens h7, h8, h12, h14, h5, h3, and h17, respectively. these cross-reactions were reciprocal. | 1991 | 1718304 |
| structure of the 021 antigen from serratia marcescens. | lipopolysaccharide was isolated from both phases of an aqueous-phenol extraction of defatted cell walls from the reference strain for serratia marcescens serogroup 021. the product from the aqueous phase was of the r type, lacking a polymeric side-chain. the polymeric fraction of the lipopolysaccharide from the phenolic phase (the 021 antigen) had a disaccharide repeating-unit with the following structure: ----4)-alpha-d-glcp-(1----4)-beta-d-manpnac-(1----. | 1991 | 1720344 |
| effect of combination therapy with recombinant human granulocyte colony-stimulating factor (rg-csf) and antibiotics in neutropenic mice unresponsive to antibiotics alone. | the effect of recombinant human granulocyte colony-stimulating factor (rg-csf) in enhancing antimicrobial chemotherapy was investigated. combined treatments of rg-csf with cefotaxime, cefazolin, fosfomycin, gentamicin or amphotericin b were evaluated in systemic infections with pseudomonas aeruginosa, escherichia coli, serratia marcescens, staphylococcus aureus and candida albicans in leucopenic mice induced by pretreatment with cyclophosphamide. administration of appropriate antibiotics afforde ... | 1991 | 1720432 |
| serotyping of serratia marcescens: detection of two new o-antigens (o25 and o26). | | 1991 | 1721816 |
| drug treatment of pneumonia in the hospital. what are the choices? | mortality and morbidity of nosocomial pneumonia remain high. successful treatment of pulmonary infections depends on several factors including type of infection, offending pathogen, status of host defences, and adequate choice of antibiotic therapy. the physician's decision should aim at achieving antibiotic concentrations beyond the mic at the site of infection. gram-negative bacilli, notably pseudomonos aeruginosa, klebsiella pneumoniae and escherichia coli, remain the most frequent agents in ... | 1991 | 1724642 |
| microbiological evaluation of cefpodoxime proxetil. | cefpodoxime, the active de-esterified molecule of the orally absorbable cephalosporin cefpodoxime proxetil, inhibits streptococci, neisseria spp., and most enterobacteriaceae, with mic50 and/or mic90 values of less than or equal to 2 mg/l; with regard to the latter family of bacteria, the mic50 and/or mic90 values of cefpodoxime are consistently greater than or equal to 4 mg/l for only enterobacter cloacae, citrobacter freundii, serratia marcescens, and morganella morganii. the mic50 of cedpodox ... | 1991 | 1726210 |
| distribution of an l-isoaspartyl protein methyltransferase in eubacteria. | a protein carboxyl methyltransferase (ec 2.1.1.77) that recognizes age-damaged proteins for potential repair or degradation reactions has been found in all vertebrate tissues and cells examined to date. this enzyme catalyzes the transfer of methyl groups from s-adenosylmethionine to the carboxyl groups of d-aspartyl or l-isoaspartyl residues that are formed spontaneously from normal l-aspartyl and l-asparaginyl residues. a similar methyltransferase has been found in two bacterial species, escher ... | 1992 | 1729230 |
| analysis of a caulobacter crescentus gene cluster involved in attachment of the holdfast to the cell. | caulobacter crescentus firmly adheres to surfaces with a structure known as the holdfast, which is located at the flagellar pole of swarmer cells and at the stalk tip in stalked cells. a three-gene cluster (hfaab and hfac) is involved in attachment of the holdfast to the cell. deletion and complementation analysis of the hfaab locus revealed two genes in a single operon; both were required for holdfast attachment to the cell. sequence analysis of the hfaab locus showed two open reading frames wi ... | 1992 | 1732204 |
| oral ofloxacin therapy for lower respiratory tract infection. | we made an open, noncomparative evaluation of ofloxacin, 400 mg orally bid for 10 days, in 98 subjects with community-acquired pneumonia or pathogen-confirmed bronchitis. thirty-nine (40%) of the subjects were treated in the hospital and 59 (60%) were treated as outpatients. the mean age of those treated was 56.2 years; 73 (74%) of the subjects either were more than 60 years old or had a history of chronic obstructive pulmonary disease, or both. there were 95 organisms initially isolated in sput ... | 1992 | 1734527 |
| restriction endonuclease mapping of the hi2 incompatibility group plasmid r478. | a restriction map of the 272-kb inchi2 plasmid r478 was constructed by using the enzymes apai, xbai, sali, and xhoi. the map was derived from cloned restriction fragments from r478 inserted into cosmid and plasmid vectors as well as from double-digestion analysis of r478 and r478 miniplasmids. all previously known resistance determinants were cloned from r478, and their positions were located on the restriction map. a region involved in incompatibility was cloned and mapped. the location of a pr ... | 1992 | 1735713 |
| chemical inactivation of microorganisms on rigid gas permeable contact lenses. | the efficiency of four contact lens disinfectant formulations was tested against three types of bacteria (pseudomonas aeruginosa, staphylococcus aureus, and serratia marcescens) and one fungus (aspergillus niger). the bacteria were tested both as free suspensions and after attachment on surfaces of rigid gas permeable contact lenses (rgpcl). the disinfection data were used to calculate the death rates and decimal reduction times for the test organisms. p. aeruginosa was the most sensitive and a. ... | 1991 | 1745499 |
| alboaggregin-b: a new platelet agonist that binds to platelet membrane glycoprotein ib. | a new protein, called alboaggregin-b (al-b), has been isolated from trimeresurus albolabris venom by ion-exchange chromatography. it agglutinated platelets without the need for ca2+ or any other cofactor. the purified protein showed an apparent molecular mass on sds-page and gel filtration of about 23 kda under nonreducing conditions. ristocetin did not alter the binding of al-b to platelets or affect al-b-induced platelet agglutination. agglutinating activity was not dependent on either proteol ... | 1991 | 1747371 |
| bloodgroup simulating activity in aerobic gram-negative oral bacteria cultured from fresh corpses. | the gram-negative aerobic oral bacterial flora of 100 consecutive corpses was isolated. after the identification and culturing of the isolated organisms, blood grouping was performed by the haemagglutination inhibition technique on dried culture smears, the dried culture medium and a dried ethanol extract of the bacteria. forty-seven of the samples showed a gram-negative aerobic bacterial growth, giving 58 microorganisms of 14 different species. positive blood grouping results were found in two ... | 1991 | 1748361 |
| septic arthritis in heroin addicts. | over a 6-year period (1982 to 1988), 36 episodes of septic arthritis were diagnosed in 35 heroin addicts from barcelona, spain. thirty (86%) were men and five (14%) were women, with a mean age of 24 years (range, 14 to 39). twenty-nine episodes (80%) were monoarticular and seven (20%) were oligoarticular. the sacroiliac (16 cases), sternoclavicular (8), hip (5), and shoulder (4) joints were most frequently infected. staphylococcus aureus and pseudomonas aeruginosa were the etiological agents in ... | 1991 | 1749942 |
| separation of the prodigiosin-localizing crude vesicles which retain the activity of protease and nuclease in serratia marcescens. | crude vesicles in which prodigiosin is localized were separated from pigmented serratia marcescens. the bacteria were grown on peptone-glycerol agar plate, suspended in saline, and fractionated into cells, vesicles, and supernatant by differential centrifugation. electron microscopic observations showed that the fractionation was conducted properly and the separated vesicles were lysed in distilled water. the vesicles suspended in saline retained 100 kilodalton protein of which amount is correla ... | 1991 | 1753881 |
| bacteriocin typing and biotyping of clinical isolates of serratia marcescens. | a total of 727 clinical isolates of serratia marcescens from 474 patients were typed for bacteriocin susceptibility; 707 of the isolates (97.25%) were typable and comprised 46 different bacteriocin types. the biotyping method of grimont and grimont permitted categorization of 726 of the 727 isolates into 50 biotype profiles. however, 98 of the 726 biotypable isolates (13.5%) yielded previously unknown biotype profiles; similarly, 24 out of 59 reference strains of s. marcescens gave biotype profi ... | 1991 | 1755921 |
| delayed serratia marcescens osteomyelitis following a gunshot injury. | | 1991 | 1757149 |
| increase of hemolysin expression in escherichia coli by cloned dna sequences of serratia marcescens. | a genomic library of sau 3ai-generated serratia marcescens dna fragments was constructed in escherichia coli 5k harbouring plasmid pann202-312. two clones capable of increasing the external hemolytic activity of the parental strain were isolated and characterized. the recombinant plasmids purified from these clones (designated ppsf29 and ppsh18) contained approximately 30 and 20 kb, respectively, of serratia dna. introduction of either plasmid in escherichia coli caused a marked increase in hemo ... | 1991 | 1760137 |
| evaluation of a biologic response modifier derived from serratia marcescens: effects on feline macrophages and usefulness for the prevention and treatment of viremia in feline leukemia virus-infected cats. | normal feline bone marrow-derived macrophages released maximum concentrations of interleukin-6, tumor necrosis factor, and interleukin-1 when stimulated with imuvert (cell technology inc, boulder, co, usa) at dosages of 1.0 microgram/ml, 5.0 micrograms/ml, and 10.0 micrograms/ml, respectively. when imuvert was administered to healthy adult cats, significant elevations in rectal temperature and neutrophil counts were observed 10 and 24 hours after each treatment. weekly treatment with imuvert fai ... | 1991 | 1768375 |
| synergic post-antibiotic effect of amikacin in combination with beta-lactam antibiotics on gram-negative bacteria. | the post-antibiotic effect (pae) of amikacin alone and in combination with ceftazidime, ceftriaxone and piperacillin was studied for two strains each of pseudomonas aeruginosa and serratia marcescens using a bioluminescent assay of bacterial atp. two models were used for combining beta-lactam antibiotics and amikacin: in one model the cultures were incubated with 32 mg/l of ceftazidime, 128 mg/l of ceftriaxone or 32 mg/l of piperacillin for 1 h. different concentrations of amikacin (0.5-64 mg/l) ... | 1991 | 1769941 |
| hiv infection among members of the army reserve components residing in new york city. | a total of 10,722 members of the us army reserve and army national guard residing within the five boroughs of new york city (nyc) were tested for antibody to human immunodeficiency virus (hiv). the crude prevalence in nyc was found to be 10.6 per 1,000, compared with a national prevalence of 1.6 per 1,000. the highest prevalences were found among blacks and hispanics, males, and among those aged 30-39 years. patterns of infection were found to be similar to those found among heterosexual and dru ... | 1991 | 1771042 |
| parathyroid cell number and size in hypocalcemic young rats. | weanling rats were fed diets with normal (1%) or low (0.08% or 0.02%, respectively) ca content for 28 days prior to sacrifice. the total volume of the parathyroids was estimated from serial sections. volume density of secretory cells was calculated according to conventional stereological techniques, whereas cell number and cell size were estimated by the dissector method. compared with controls the animals of the experimental groups developed moderate and severe hypocalcemia and their parathyroi ... | 1991 | 1772646 |
| beta-lactamase expression and outer membrane protein changes in cefpirome-resistant and ceftazidime-resistant gram-negative bacteria. | twenty-five strains of enterobacteriaceae (five each of enterobacter cloacae, citrobacter freundii, serratia marcescens, morganella morganii, and providencia stuartii) and five strains of pseudomonas aeruginosa were exposed to various concentrations of cefpirome or ceftazidime in agar. mutants with a greater than four-fold increase in the mic were examined for changes in beta-lactamase expression and outer membrane protein (omp) profile. both agents selected mutants with decreased susceptibility ... | 1991 | 1778852 |
| selection and characterization of cefepime-resistant gram-negative bacteria. | the nctc type strains and four clinical isolates of enterobacter cloacae, citrobacter freundii, serratia marcescens, morganella morganii, providencia stuartii and pseudomonas aeruginosa were exposed, in agar, to cefepime at 3, 5, and 10 x mic and a breakpoint concentration of 16 mg/l. mutants were selected at a frequency of approximately 10(-8) that had decreased susceptibility to cefepime and cefpirome, and species-dependent resistance to other beta-lactams. any putative mutant with a greater t ... | 1991 | 1778870 |
| excretion of the escherichia coli alpha-haemolysin by serratia marcescens. | serratia marcescens 2170 derivative strains harbouring either the haemolytic plasmid pann202-312 or pann202-312r, were able to produce extracellular haemolysin to a greater extent than the corresponding escherichia coli 5k strains. the haemolysin recovered from s. marcescens cultures supernatants had a molecular weight of 110 kd, and its extracellular production was maximal near the end of the exponential growth phase. the hlyr sequence, which belongs to the haemolytic determinant located in the ... | 1991 | 1779876 |
| growth of mouse ectoplacental cone cells in subcutaneous tissues. development of placental-like cells. | ectoplacental cones of mouse embryos collected on day 8 of pregnancy were grafted into the dorsal subcutaneous tissue of host mice. the grafts were collected between days 3 and 8 after transfer and processed for light and electron microscope morphological analysis as well as for cytochemistry of nonspecific alkaline phosphatase. fragments of normal mouse placentas collected between days 12 and 18 of pregnancy were processed similarly. about 37% of the grafts were nonhemorrhagic nodules formed by ... | 1991 | 1781448 |
| antimicrobial effects from incorporation of disinfectants into gypsum casts. | five microorganisms--escherichia coli, streptococcus mutans, staphylococcus aureus, serratia marcescens, and bacillus subtilis--were placed into the canine, first premolar, first molar, and second molar of sterilized quadrant addition-reaction silicone impressions. four disinfectant solutions--iodophor, neutral glutaraldehyde, phenol, and sodium hypochlorite--were spatulated with sterilized type iv stone and vibrated into the intentionally contaminated impressions. the casts were removed at 1 an ... | 1991 | 1781882 |
| effects of testosterone on the liver of castrated rats: morphologic study. | it is well known that castration in rats causes atrophy of the sexual organs and, to a lesser extent, of other organs. we have observed that castration causes a reduction of the liver weight due to a reduction (-10%) of the numerical density of the hepatocytes after castration (p less than 0.01). testosterone administration at high concentration (2.5 mg/100 g b. wt.) as well as at low concentration (0.025 mg/100 g) in the castrated rats causes an increase of the liver weight due to an increase ( ... | 1991 | 1783042 |
| entomopathogens of phlebotomine sand flies: laboratory experiments and natural infections. | the susceptibility of different geographical strains of phlebotomus papatasi to a cytoplasmic polyhedrosis virus (cpv) was determined experimentally by feeding polyhedra to larvae. of the indian p. papatasi, 15.6% became infected, whereas egyptian p. papatasi were mostly refractory. infection rates were not augmented in colony flies from the jordan valley, 23.8% of which were naturally infected with cpv. the infectivity of serratia marcescens and beauvaria bassiana to p. papatasi were determined ... | 1991 | 1783777 |
| [clinical response of chemotherapeutic agents with different activities on chronic complicated pyelonephritis with renal stone. a proposed index of local antimicrobial activity]. | in the treatment of one male patient with chronic pyelonephritis, complicated with renal stone, the pathological state of the renal inflammatory lesion was determined. the patient had been persistently infected by the same strain of s. marcescens for more than a year. when he was treated by several antimicrobial agents, the urinary bacteriological response was well correlated to the mics of each agent. on the basis of the findings obtained, a new index of local antimicrobial activity was propose ... | 1991 | 1785421 |
| [work capacity of man when using individual respiratory protective devices]. | gas-mask decreased the ability to work by 20-50%. it was caused by decreasing skin and respiratory evaporation. ventilation of the under mask interspace (90 1/min) was recommended. | 1991 | 1790865 |
| [spheroplast formation in clinical isolates of gram-negative bacteria by beta-lactam antibiotic in the presence of ca2+ or mg2+]. | induction to cell wall-deficient bacteria has been suggested to be a cause of intractable and opportunistic infection after chemotherapy. spheroplast formation by beta-lactam antibiotic in not so high osmotic pressure was investigated in six species of gram-negative bacteria. some species of gram-negative bacteria were induced to form spheroplast at a high rate by 1:10 concentration of ceftizoxime in the presence of ca2+ or mg2+. especially in 38% of proteus mirabilis and p. vulgaris, more than ... | 1991 | 1791327 |
| structure of an acidic glycan present in the lipopolysaccharide extract from the reference strain for serratia marcescens serogroup o18. | the lipopolysaccharide extract from the cell wall of the reference strain for serratia marcescens serogroup o18 contained, in addition to a neutral glycan characterised previously, an acidic glycan. acidity was contributed both by d-glucuronic acid and by 4-o-[(r)-1-carboxyethyl]-d-glucose (4-o-lac-d-glc). by using n.m.r. spectroscopy, methylation analysis, and chemical degradations, the repeating unit of the acidic glycan was identified as a branched hexasaccharide having the structure shown; a ... | 1991 | 1794127 |
| post-antibiotic effect of beta-lactam antibiotics on gram-negative bacteria in relation to morphology, initial killing and mic. | the in vitro post-antibiotic effect (pae) of cefepime, cefotaxime, ceftazidime and imipenem on reference strains of escherichia coli, enterobacter cloacae, klebsiella pneumoniae, pseudomonas aeruginosa and serratia marcescens were evaluated by bioluminescence assay of bacterial atp. in parallel with the pae determination, initial killing and morphology studies were performed. imipenem produced greater than 1 h pae on all strains tested, cefepime and cefotaxime on four strains and ceftazidime onl ... | 1991 | 1794362 |
| the effect of exposure to impulse x rays on normal and epileptic activity in rabbit brain. | exposure to x rays (20 impulses of 4 hz frequency, total dose 0.6-1.1 mgy) increased the epileptic activity of a focal area, which was produced in the visual cortex of rabbit brain by freezing with liquid nitrogen, and by stimulating with flashes of light at frequencies of 5-6 hz. the number of seizure complexes during photostimulation for 5 s increased by 80% compared with the initial level, and this effect continued for 15 min. in control animals (with no epileptic foci), a decrease was observ ... | 1991 | 1797142 |
| the effect of exposure to impulse x rays on normal and epileptic activity in rabbit brain. | exposure to x rays (20 impulses of 4 hz frequency, total dose 0.6-1.1 mgy) increased the epileptic activity of a focal area, which was produced in the visual cortex of rabbit brain by freezing with liquid nitrogen, and by stimulating with flashes of light at frequencies of 5-6 hz. the number of seizure complexes during photostimulation for 5 s increased by 80% compared with the initial level, and this effect continued for 15 min. in control animals (with no epileptic foci), a decrease was observ ... | 1991 | 1797142 |
| in vitro activity of a new cephalosporin me-1206 compared with other agents. | the in vitro activity of me-1206, a new aminothiazolyl cephalosporin that can be orally absorbed when converted to an ester, was compared with that of other beta-lactams. me-1206 inhibited 50% of the enterobacteriaceae at 2 micrograms/ml, similar to cefotaxime, ceftazidime, and cefixime. it did not inhibit, mic greater than or equal to 32 micrograms/ml, enterobacter species or citrobacter freundii resistant to cefotaxime and ceftazidime, and it was less active than cefotaxime and ceftazidime aga ... | 1991 | 1797456 |
| in vitro activity evaluations of cefdinir (fk482, ci-983, and pd134393). a novel orally administered cephalosporin. | cefdinir, a so-called third-generation oral cephalosporin was tested in vitro against over 700 pathogens from patients with bacteremia. cefdinir was very active against the enterobacteriaceae with a 50% minimum inhibitory concentration (mic50) value range of less than or equal to 0.03-8 micrograms/ml. the enteric species having the highest mic90s (greater than or equal to 16 micrograms/ml) were citrobacter freundii, and the enterobacters, morganella morganii, proteus vulgaris, and serratia marce ... | 1991 | 1797457 |
| oral ofloxacin therapy of infections due to multiply-resistant bacteria. | we determined the efficacy and safety of orally administered ofloxacin, 400 mg twice daily, in the treatment of infections due to multiply-resistant bacteria. patients (n = 99) were treated for 84 infections in 82 patients evaluable for efficacy with a bacteriologic response of 71%. organisms treated included pseudomonas aeruginosa (39), staphylococcus aureus (11), serratia marcescens (9), enterobacter species (7), five each of escherichia coli, citrobacter, salmonella, klebsiella, and other org ... | 1991 | 1797458 |
| [susceptibilities of clinical bacterial isolates to antimicrobial agents, 1989. a study mainly focused on imipenem. the research group for testing imipenem susceptibilities of clinical isolates]. | we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at hospital laboratories throughout japan from september to december of 1989. the susceptibility testing was carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: (+++), (++), (+) and (-). ipm showed markedly high in vitro activities against streptococcus pneumoniae, neisseria gonorrhoeae, moraxella catarrhalis, es ... | 1991 | 1798067 |
| the enzymatic profile of urine and plasma in bovine urinary bladder cancer (enzootic bovine haematuria). | the enzymatic profile of urine and plasma in field cases of bovine bladder cancer was studied. urinary lactate dehydrogenase activity was significantly altered along with the isoenzyme pattern. activity of alkaline phosphatase and beta-glucuronidase was decreased in the affected animals. no significant changes were observed in acid phosphatase, or arylsulphatase a and b activity. in plasma, lactate dehydrogenase activity was elevated without any change in the isoenzyme pattern. no significant ch ... | 1991 | 1803721 |
| purification of equine neutrophil lysozyme and its antibacterial activity against gram-positive and gram-negative bacteria. | lysozyme from equine neutrophil granulocytes was isolated in a pure form by fast performance liquid chromatography, i.e. ion-exchange chromatography and reversed-phase chromatography. the lysozyme lysed micrococcus luteus, bacillus subtilis and staphylococcus lentus and was also bactericidal against the gram-negative bacteria escherichia coli, klebsiella pneumoniae, bordetella bronchiseptica, and serratia marcescens. staphylococcus aureus and staphylococcus epidermidis were not lysed. the lysozy ... | 1991 | 1803722 |
| effect of clavulanic acid on activity of beta-lactam antibiotics in serratia marcescens isolates producing both a tem beta-lactamase and a chromosomal cephalosporinase. | an isolate of serratia marcescens that produced both an inducible chromosomal and a plasmid-mediated tem-1 beta-lactamase was resistant to ampicillin and amoxicillin and also demonstrated decreased susceptibility to extended-spectrum beta-lactam antibiotics (esbas). clavulanic acid did not lower the mics of the esbas, but it decreased the mics of the penicillins. the tem-1-producing plasmid was transferred to a more susceptible s. marcescens strain that produced a well-characterized inducible ch ... | 1991 | 1803992 |
| in vitro studies with five quinolones: evidence for changes in relative potency as quinolone resistance rises. | a panel of 203 staphylococci, enterobacteriaceae, pseudomonas aeruginosa, and miscellaneous nonfermentative gram-negative bacilli were chosen for their various susceptibilities to ciprofloxacin. on the basis of agar dilution susceptibilities, each of the four taxonomic groups was divided into ciprofloxacin-susceptible, moderately resistant, and highly resistant subgroups, and each subgroup was then further analyzed for its susceptibility to the fluoroquinolones ci-960, ci-990, sparfloxacin, and ... | 1991 | 1804005 |
| [the protective effects of paeonol on cultured neonatal rat heart cells during calcium paradox]. | it has been found that the rapid (5min) and slow (120min) phases of 15ca uptake by normal myocardial cells and their beating rate are remarkably inhibited by paeonol. and that paeonol can also decrease 45ca uptake and lipid peroxide in calcium paradoxical heart cells, which appears in dose-dependence. this indicates that the mechanism of protection of paconol against calcium paradox is associated with its inhibition of ca2+ influx and antioxidation. | 1991 | 1804206 |
| in vitro and in vivo activity of carbamate-linked dual-action antibacterial ro 24-4383. | ro 24-4383 contains desacetylcefotaxime linked by a carbamate bond at the 3' position to ciprofloxacin. ro 24-4383 was active against 99% of the 363 gram-positive and gram-negative aerobes tested in vitro, while the comparative agents cefotaxime and ciprofloxacin were active against 77 and 97%, respectively. the activities (ed50: mg/kg s.c.) of ro 24-4383, cefotaxime and ciprofloxacin in systemic murine infections were: escherichia coli 257, 1.4, less than 0.5, less than 0.2; klebsiella pneumoni ... | 1991 | 1804592 |
| [the use of alveolar macrophages in the hygienic standardization of industrial strains of microorganisms]. | | 1991 | 1809655 |
| antimicrobial activity of ru29246 (hr916 metabolite) compared with four other oral beta-lactams tested against more than 5000 clinical isolates. | the activity of ru29246, the active metabolite of the oral cephalosporin ester hr916, was compared in a multicenter study with that of the four oral beta-lactam antibiotics cephalexin, cefaclor, cefixime and amoxicillin/clavulanate (amoxicillin/ca). ru29246 was generally 2- to 8-fold more active than the other oral cephalosporins and comparable to amoxicillin/ca against staphylococci, and was the most active cephalosporin against group b streptococci. all four cephalosporins were ineffective aga ... | 1991 | 1810737 |
| reduction of capsular polysaccharide and potentiation of aminoglycoside inhibition in gram-negative bacteria by bismuth subsalicylate. | bismuth subsalicylate (bss), sodium salicylate, and bismuth nitrate were compared with respect to their effects on capsular polysaccharide (cps) production, bacterial growth inhibition, and potentiation of aminoglycoside inhibition on strains of gram-negative bacteria. at 250 microm, bss reduced cps production in klebsiella pneumoniae cultures by greater than 90% in contrast to a 36% reduction by salicylate. at 500 microm, salicylate reduced cps by 52%, versus a 70% reduction by bismuth nitrate. ... | 1991 | 1816178 |
| one year follow-up of a hiv seropositive jail population. | a jail population has been followed for one year to study the risk of hiv-seroconversion. our data show that life in jail is not necessarily a condition for hiv spread. in fact no variation of seropositivity distribution has been found. | 1991 | 1818517 |
| spreading of serratia marcescens in experimental keratitis and growth suppression by chicken egg white ovomacroglobulin. | we studied the inhibitory effects of chicken egg white ovomacroglobulin, a broad-spectrum protease inhibitor, and a synthetic protease inhibitory peptide, bz-gfr-o-mercaptoanilide, on the growth of two strains of serratia marcescens in a synthetic medium. the growth of the virulent protease-producing strain, s. marcescens kums 3958 (kums 3958), was more rapid than that of the strain producing minimal protease, s. marcescens kums na (kums na), and kums 3958 was inhibited in a dose-dependent manne ... | 1991 | 1821430 |
| imuvert therapy in the treatment of recurrent malignant astrocytomas: nursing implications. | malignant astrocytoma is a fatal, primary brain tumor affecting adults in the prime of life. even with optimal treatment, median life expectancy from time of diagnosis is 51 weeks. standard therapy consists of surgical debulking, radiation therapy and chemotherapy. immunotherapy, utilizing biologic response modifiers (brms), affords one of the more promising treatment modalities. imuvert, a brm derived from serratia marcescens, was recently granted orphan drug status by the food and drug adminis ... | 1991 | 1826714 |
| the retinoblastoma protein copurifies with e2f-i, an e1a-regulated inhibitor of the transcription factor e2f. | recently, we identified an inhibitory protein, e2f-i, that blocks the dna-binding activity of the transcription factor e2f. we also showed that the adenovirus e1a protein reverses this inhibitory activity of e2f-i, thereby restoring the dna-binding activity of e2f. we have now further purified this inhibitory activity and show that the most purified preparation of e2f-i contains a 105 kd e1a-binding protein. this 105 kd e1a-binding protein cross-reacts with two different antibodies against the r ... | 1991 | 1828393 |
| the retinoblastoma protein copurifies with e2f-i, an e1a-regulated inhibitor of the transcription factor e2f. | recently, we identified an inhibitory protein, e2f-i, that blocks the dna-binding activity of the transcription factor e2f. we also showed that the adenovirus e1a protein reverses this inhibitory activity of e2f-i, thereby restoring the dna-binding activity of e2f. we have now further purified this inhibitory activity and show that the most purified preparation of e2f-i contains a 105 kd e1a-binding protein. this 105 kd e1a-binding protein cross-reacts with two different antibodies against the r ... | 1991 | 1828393 |
| [classification of sensitive and resistant strains based on the degree of antibiotic growth inhibition]. | the course of growth suppression of sensitive strains depends on the type of antibiotic. based on the kinetics of the early growth phase in the microculture when the inoculum is relatively concentrated it is possible to divide in the presence of the antibiotic the gram negative fermenting rods into sensitive and resistant strains already within five hours. data obtained by automated photometric assessment at selected intervals were processed by the aggregation method using a block distance as th ... | 1991 | 1828715 |
| surveys of human enterotoxigenic escherichia coli from three different geographical areas for possible colonization factors. | enterotoxigenic escherichia coli (etec) from burma, central africa (rwanda and zaire) and peru, were screened by enzyme-linked immunoassays for the colonization factor antigens (cfas) and putative colonization factors (pcfs): cfa/i, cfa/ii, which consists of three coli surface-associated (cs) antigens, cs1, cs2 and cs3, cfa/iii, cfa/iv (cs4, cs5, cs6), cs7, pcfo9, pcfo159. h4, pcfo166, and cs17. the highest proportion of etec with identifiable colonization factors (71%) were found in the strains ... | 1991 | 1828771 |
| contribution of serotonin neurons to the functional recovery after spinal cord injury in rats. | the contribution of serotonin neurons to the functional recovery after spinal cord injury was studied pharmacologically in rats with moderately severe neurologic impairment (complete paraplegia but responsive to tail pinching) 24 h after thoracic spinal cord (t11) compression-induced injury. fourteen days after cord injury the levels of endogenous norepinephrine (ne, -33%), dopamine (da, -50%) and serotonin (5-ht, -55%) in the lumbar cord in the injury control rats were decreased and there were ... | 1991 | 1829012 |
| contribution of serotonin neurons to the functional recovery after spinal cord injury in rats. | the contribution of serotonin neurons to the functional recovery after spinal cord injury was studied pharmacologically in rats with moderately severe neurologic impairment (complete paraplegia but responsive to tail pinching) 24 h after thoracic spinal cord (t11) compression-induced injury. fourteen days after cord injury the levels of endogenous norepinephrine (ne, -33%), dopamine (da, -50%) and serotonin (5-ht, -55%) in the lumbar cord in the injury control rats were decreased and there were ... | 1991 | 1829012 |
| age-related differences in phosphonoformate-induced bone toxicity in cats. | phosphonoformate (pfa), a monophosphonate pyrophosphate analog, caused plasma biochemical and bone histomorphologic abnormalities in cats given 1,000 mg/kg/day as a continuous intravenous infusion for 14 days. plasma biochemical alterations observed in young cats (10 weeks old) treated with pfa included increased calcium and decreased phosphorus, alkaline phosphatase, and calcitriol. young cats treated with pfa developed rickets-like lesions characterized by widened growth plates, increased oste ... | 1991 | 1829019 |
| age-related differences in phosphonoformate-induced bone toxicity in cats. | phosphonoformate (pfa), a monophosphonate pyrophosphate analog, caused plasma biochemical and bone histomorphologic abnormalities in cats given 1,000 mg/kg/day as a continuous intravenous infusion for 14 days. plasma biochemical alterations observed in young cats (10 weeks old) treated with pfa included increased calcium and decreased phosphorus, alkaline phosphatase, and calcitriol. young cats treated with pfa developed rickets-like lesions characterized by widened growth plates, increased oste ... | 1991 | 1829019 |
| defective and nondefective adenovirus vectors for expressing foreign genes in vitro and in vivo. | we have constructed recombinant adenoviruses (ad), with functional or defective e1a genes, which harbor either the hepatitis b (hb) virus s gene encoding the hb surface antigen, as well as the pre-s2 epitopes, or the bacterial gene encoding chloramphenicol acetyltransferase (cat) under control of the ad major late promoter (mlp). the recombinant viruses defective for e1a (ad.mlp.s2 and ad.cat), which can be efficiently propagated only on 293 cells that complement this defect, and the nondefectiv ... | 1991 | 1829048 |
| defective and nondefective adenovirus vectors for expressing foreign genes in vitro and in vivo. | we have constructed recombinant adenoviruses (ad), with functional or defective e1a genes, which harbor either the hepatitis b (hb) virus s gene encoding the hb surface antigen, as well as the pre-s2 epitopes, or the bacterial gene encoding chloramphenicol acetyltransferase (cat) under control of the ad major late promoter (mlp). the recombinant viruses defective for e1a (ad.mlp.s2 and ad.cat), which can be efficiently propagated only on 293 cells that complement this defect, and the nondefectiv ... | 1991 | 1829048 |
| combination of long and short term gnrh analogue protocols: a new therapeutic approach to persistent high progesterone levels in ivf cycles. | thirty-four patients who had had persistently high progesterone (p) levels during menotrophin therapy in previous in-vitro fertilization (ivf) cycles, despite pretreatment with a long acting gonadotrophin-releasing hormone analogue (gnrha), were randomly divided into two groups according to the mode of ovulation induction used. in 16 women (group 1), induction of ovulation was performed with decapeptyl (dtrp6)/pure follicle-stimulating hormone (pfsh)/human menopausal gonadotrophin (hmg)/human ch ... | 1991 | 1829091 |
| a generalized transducing bacteriophage for serratia marcescens. | using serratia marcescens 2170 harbouring plasmid ptroy11 (encoding for lamb), and lambda (lambda:: tn5) we constructed a collection of 25 auxotrophic mutations induced by tn5 insertions. these mutants permitted the use an easy screening method for generalized transducing bacteriophages. out of twelve bacteriophages isolated from natural sources only one (phage 3m) was able to transduce all tn5 insertions tested. the preliminary characterization of bacteriophage 3m indicates that it belongs to t ... | 1991 | 1829848 |
| pharmacodynamics of amikacin in vitro and in mouse thigh and lung infections. | a variety of in-vitro and animal studies were performed to define the pharmacodynamic characteristics of amikacin against gram-negative bacilli and to determine the impact of the dosing regimen on therapeutic efficacy. mice with impaired renal function were included in these studies to more closely simulate in animals the pharmacokinetics of amikacin observed in humans. amikacin exhibited concentration-dependent killing and produced prolonged postantibiotic effects both in vitro and in vivo. in ... | 1991 | 1830302 |
| [resistance of hospital flora to imipenem. experience in two intensive care units]. | imipenem is a beta-lactam antibiotic active against most gram-negative bacilli. between july 1, 1987 and september 30, 1989 (9 semesters), the activity of imipenem against 6 micro-organisms was tested in two intensive care units attached to the university hospital of caen (normandy). during the same period, the consumption of imipenem was evaluated from the number of vials drawn by each of these two units from the central pharmacy. imipenem was found to be 100 percent effective against 5 of the ... | 1991 | 1835072 |
| the tonb gene of serratia marcescens: sequence, activity and partial complementation of escherichia coli tonb mutants. | the tonb protein plays a key role in the energy-coupled transport of iron siderophores, of vitamin b12, and of colicins of the b-group across the outer membrane of escherichia coli. in order to obtain more data about which of its particular amino acid sequences are necessary for tonb function, we have cloned and sequenced the tonb gene of serratia marcescens. the nucleotide sequence predicts an amino acid sequence of 247 residues (mr 27,389), which is unusually proline-rich and contains the tand ... | 1991 | 1838128 |
| [sarcocystis species in vietnamese workers]. | the authors give an account of the finding of sporocysts of the sarcocystis spp. in 14 vietnamese apprentices from a total of 1228 examined (1.1%) who came to central slovakia in the course of 18 months in 1987-1989. the mean period of sporocyst excretion was 49.2 days. the positive subjects did not report gastrointestinal complaints despite mixed intestinal parasitoses. the subjects were from the north eastern part of the country from hanoi-haiphong areas and they did not report that they ate r ... | 1991 | 1838711 |
| partial characterization of serratia marcescens nosocomial strains. | a nosocomial infection outbreak occurred in the intensive care unit (icu) of the instituto nacional de pediatría (inp) in méxico city, during the months of march, april and may in 1988 serratia marcescens was isolated as the etiological agent for this epidemic. up to date, the source of contamination, the spreading and the pathogenic mechanisms which were involved in this outbreak remain unknown. in order to study the dynamics of the bacterial population involved in this outbreak, all strains of ... | 1991 | 1844112 |
| absence of na+,k(+)-atpase regulation of endosomal acidification in k562 erythroleukemia cells. analysis via inhibition of transferrin recycling by low temperatures. | transferrin (tf) acidification has been shown to be limited to ph 6 in murine balb/c 3t3 fibroblasts, human a549 epidermoid carcinoma cells, and chinese hamster ovary cells and is followed by alkalinization during recycling. in contrast, tf acidification in the human erythroleukemic cell line k562 proceeds to below ph 5.5, and alkalinization of internal tf during recycling is not observed. to explore the regulation of endosomal ph in k562 cells, we determined whether the existence of an early en ... | 1991 | 1847374 |