| catabolite repression of the citrate fermentation genes in klebsiella pneumoniae: evidence for involvement of the cyclic amp receptor protein. | klebsiella pneumoniae is able to grow anaerobically with citrate as a sole carbon and energy source by a fermentative pathway involving the na(+)-dependent citrate carrier cits, citrate lyase, and oxaloacetate decarboxylase. the corresponding genes are organized in the divergent citc and cits operons, whose expression is strictly dependent on the citrate-sensing cita-citb two-component system. evidence is provided here that the citrate fermentation genes are subject to catabolite repression, sin ... | 2001 | 11514506 |
| complete nucleotide sequence and organization of the atrazine catabolic plasmid padp-1 from pseudomonas sp. strain adp. | the complete 108,845-nucleotide sequence of catabolic plasmid padp-1 from pseudomonas sp. strain adp was determined. plasmid padp-1 was previously shown to encode atza, atzb, and atzc, which catalyze the sequential hydrolytic removal of s-triazine ring substituents from the herbicide atrazine to yield cyanuric acid. computational analyses indicated that padp-1 encodes 104 putative open reading frames (orfs), which are predicted to function in catabolism, transposition, and plasmid maintenance, t ... | 2001 | 11544232 |
| novel gene cassettes and integrons. | an increase in multiresistant enterobacteriaceae was observed at one of the departments of the university medical center utrecht. nine different integrons and 17 gene cassettes were found, including the new gene cassette aada8. this cassette was highly related to aada3 and aada2. in addition, an unknown promoter sequence was found for two integrons. | 2001 | 11557503 |
| a matter of bacterial life and death. | over 50 years ago, standard microbiological methods were established for determining whether bacterial cells were dead or alive. recently there has been a flurry of reports suggesting that bacteria may exist in an eclipsed state, escaping detection by standard methods. whether there really is such a state is of more than academic interest, considering the implications for public health. the ensuing debate has been unusually energetic for the normally cultured community of microbiologists. | 2001 | 11559589 |
| electrochemical biosensor array for the identification of microorganisms based on lectin-lipopolysaccharide recognition. | rapid identification of bacterial strains remains a well-known problem in applied medicine and, for viable pathogens, is an important diagnostic goal. we have investigated an electrochemical biosensor array, in which transduction is based on respiratory cycle activity measurements, where the microorganism's native respiratory chain is interrupted with non-native external oxidants. the selective biochemical recognition agents employed in this study are lectins that, once immobilized, recognize an ... | 2001 | 11569815 |
| crystal structure of klebsiella aerogenes uree, a nickel-binding metallochaperone for urease activation. | uree is proposed to be a metallochaperone that delivers nickel ions to urease during activation of this bacterial virulence factor. wild-type klebsiella aerogenes uree binds approximately six nickel ions per homodimer, whereas h144*uree (a functional c-terminal truncated variant) was previously reported to bind two. we determined the structure of h144*uree by multi-wavelength anomalous diffraction and refined it to 1.5 a resolution. the present structure reveals an hsp40-like peptide-binding dom ... | 2001 | 11591723 |
| [utility of prolonged incubation and terminal subcultures of blood cultures from immunocompromised patients]. | the value of blind terminal subcultures (7 and 30 days) and prolonged incubation (30 days) of blood cultures from immunosuppressed patients was analyzed in the fundación favaloro, the fundación para la lucha contra las enfermedades neurológicas de la infancia and the hospital de niños ricardo gutiérrez. a total of 2707 blood cultures and 369 patients were included (transplantation of solid organs 154, oncohematologic disorders 106 and solid tumors 109). bact-alert bottles were incubated at 35 de ... | 2001 | 11594009 |
| dual effects of ionic strength on klebsiella aerogenes urease: ph-dependent activation and inhibition. | | 2001 | 11594743 |
| [spread of enterobacteriaceae producing broad-spectrum beta-lactamase and the development of their incidence over a 16-month period in a university hospital center]. | enterobacteriaceae producing extended-spectrum beta-lactamases (esble) constitute with methicillin-resistant staphylococcus aureus the main multiresistant bacteria recovered in french hospitals. our objectives were to evaluate these esble diffusion in our teaching hospital and to follow their incidence during a 16-month period, whereas a control programme (barrier precautions) had been implemented in the beginning of 1999. this study was conducted in a teaching hospital containing 1800 beds, fro ... | 2001 | 11642012 |
| dinuclear nickel(ii) complexes as models for the active site of urease. | dinuclear nickel(ii) complexes of the ligands 2,6-bis[bis((2-benzimidazolylmethyl)amino)methyl]-p-cresol (bbapoh), n,n,n',n'-tetrakis(2-benzimidazolylmethyl)-2-hydroxy-1,3-diaminopropane (tbpoh), n-methyl-n,n',n'-tris(2-benzimidazolylmethyl)-2-hydroxy-1,3-diaminopropane (m-tbpoh) and 1-[n,n-bis(2-benzimidazolylmethyl)amino]-3-[2-(3,5-dimethyl-1h-pyrazol-1-yl)ethoxy]-2-hydroxypropane (bpepoh) were prepared in order to model the active site of urease. the novel asymmetric structures of the dinucle ... | 1996 | 11666566 |
| roles of glutamate synthase, gltbd, and gltf in nitrogen metabolism of escherichia coli and klebsiella aerogenes. | mutants of escherichia coli and klebsiella aerogenes that are deficient in glutamate synthase (glutamate-oxoglutarate amidotransferase [gogat]) activity have difficulty growing with nitrogen sources other than ammonia. two models have been proposed to account for this inability to grow. one model postulated an imbalance between glutamine synthesis and glutamine degradation that led to a repression of the ntr system and the subsequent failure to activate transcription of genes required for the us ... | 2001 | 11673431 |
| pcr analyses of trna intergenic spacer, 16s-23s internal transcribed spacer, and randomly amplified polymorphic dna reveal inter- and intraspecific relationships of enterobacter cloacae strains. | pcr analysis of trna intergenic spacer (tdna-pcr) and of the 16s-23s internal transcribed spacer (its-pcr) and random amplified polymorphic dna (rapd) analysis were evaluated for their usefulness in characterization of enterobacter cloacae strains isolated from both clinical origins and vaccine microbial contamination. tdna-pcr presented specific and reproducible patterns for enterobacter sakazakii atcc 29004, enterobacter aerogenes atcc 13048, and enterobacter cloacae atcc 13047 and 23355 that ... | 2001 | 11682499 |
| new pyridoquinoline derivatives as potential inhibitors of the fluoroquinolone efflux pump in resistant enterobacter aerogenes strains. | enterobacter aerogenes, one of the most frequently isolated nosocomial pathogens in france, is exhibiting increasing multidrug resistance mechanisms associated with a change in membrane permeability. for drugs of the quinolone family, mutations in the target and active efflux play a prominent role in the resistance. we report here the effect of several pyridoquinoline derivatives that restore a noticeable fluoroquinolone accumulation to resistant strains that overexpress the mara activator. stud ... | 2001 | 11689091 |
| benzoate fermentation by the anaerobic bacterium syntrophus aciditrophicus in the absence of hydrogen-using microorganisms. | the anaerobic bacterium syntrophus aciditrophicus metabolized benzoate in pure culture in the absence of hydrogen-utilizing partners or terminal electron acceptors. the pure culture of s. aciditrophicus produced approximately 0.5 mol of cyclohexane carboxylate and 1.5 mol of acetate per mol of benzoate, while a coculture of s. aciditrophicus with the hydrogen-using methanogen methanospirillum hungatei produced 3 mol of acetate and 0.75 mol of methane per mol of benzoate. the growth yield of the ... | 2001 | 11722901 |
| shellac formulations to reduce epiphytic survival of coliform bacteria on citrus fruit postharvest. | survival of the coliform bacteria enterobacter aerogenes and escherichia coli was monitored in a neutral carboxymethylcellulose formulation and in shellac formulations with various ph and concentrations of ethanol and the preservative paraben; populations were subsequently measured from the surface of citrus fruit coated with these formulations. numbers of the two bacteria increased over 24 h from 10(6) cfu/ml to approximately 10(8) cfu/ml in the carboxymethylcellulose solution, but over this ti ... | 2001 | 11726155 |
| utilization of l-ascorbate by escherichia coli k-12: assignments of functions to products of the yjf-sga and yia-sgb operons. | escherichia coli k-12 can ferment l-ascorbate. the operon encoding catabolic enzymes in the utilization of l-ascorbate (ula) has been identified; this operon of previously unknown function had been designated the yif-sga operon. three enzymes in the pathway that produce d-xylulose 5-phosphate have been functionally characterized: 3-keto-l-gulonate 6-phosphate decarboxylase (ulad), l-xylulose 5-phosphate 3-epimerase (ulae), and l-ribulose 5-phosphate 4-epimerase (ulaf). several products of the yi ... | 2002 | 11741871 |
| evaluation of ppk-specified polyphosphate as a mercury remedial tool. | to evaluate the utility of polyphosphate kinase gene (ppk)-specified polyphosphate in mercury remediation, a fusion plasmid, pmk27, with ppk from klebsiella aerogenes and mercury transport genes, mert and merp, from pseudomonas k-62, was constructed. the transcription and translation of ppk, mert and merp were found to be mercury-inducible. the ppk-specified polyphosphate was identified in cells preinduced by hg2+, but not in cells without mercury induction, suggesting that the synthesis of poly ... | 2001 | 11767115 |
| novel alkylsulfatases required for biodegradation of the branched primary alkyl sulfate surfactant 2-butyloctyl sulfate. | recent reports show that contrary to common perception, branched alkyl sulfate surfactants are readily biodegradable in standard biodegradability tests. we report here the isolation of bacteria capable of biodegrading 2-butyloctyl sulfate and the identification of novel enzymes that initiate the process. enrichment culturing from activated sewage sludge yielded several strains capable of growth on 2-butyloctyl sulfate. of these, two were selected for further study and identified as members of th ... | 2002 | 11772605 |
| comparative in vitro activity of isepamicin and other antibiotics against gram-negative bacilli from intensive care units (icu) in belgium. | the in vitro activity of isepamicin was compared to that of amikacin, gentamicin, cefepime, ciprofloxacin and meropenem against gram-negative bacilli isolated from patients hospitalized in the intensive care unit (icu) of 11 belgian university or general hospitals between november 1998 and july 1999. the minimal inhibitory concentrations (mic) for 1087 non-duplicate, consecutive aerobic gram-negative isolates, including 798 enterobacteriaceae and 289 non-fermenters, were determined by e-test for ... | 2001 | 11774843 |
| ethnobotanical and antimicrobial investigation on some species of terminalia and combretum (combretaceae) growing in tanzania. | an ethnobotanical investigation on the medicinal uses of some species of terminalia and combretum (combretaceae) was carried out in mbeya, tanzania during a 5-weeks field expedition. of the sixteen species collected, combretum fragrans f. hoffm., combretum molle g. don., combretum psidioides welw., combretum zeyheri sond., terminalia kaiserana f. hoffm. and terminalia sericea burch ex. dc. have medical applications against various bacterial infections, such as gonorrhoea and syphilis, and agains ... | 2002 | 11801378 |
| crystallization and preliminary x-ray structure determination of jack bean urease with a bound antibody fragment. | urease allows organisms to use exogenous and internally generated urea as a nitrogen source, by catalyzing the hydrolysis of urea to form ammonia and carbon dioxide. urease may also participate in the systemic nitrogen-transport pathways and possibly acts as a toxic defence protein. jack bean urease (jbu) was the first nickel-metalloenzyme identified and was crystallized as early as 1926. despite this, the structure has not yet been determined. an antibody fragment, fv, that has a high affinity ... | 2002 | 11807281 |
| relationship between teat-end callosity and occurrence of clinical mastitis. | a longitudinal study in 15 herds, with a total of 2157 cows, was conducted to examine the relationship between teat-end callosity (tec) and the incidence of clinical mastitis. during the 1.5-yr study period, clinical mastitis was diagnosed by the farmers based on clinical signs. teat-end callosity was scored every month according to a teat-end callosity classification system, which discriminates between teat-end callosity thickness (tect) and roughness (tecr). differences in tect between healthy ... | 2001 | 11814022 |
| antimicrobial susceptibility of respiratory isolates of enterobacteriaceae and staphylococcus aureus in italy: incidence and trends over the period 1997-1999. | the antibiotic susceptibility of members of the family enterobacteriaceae and of staphylococcus aureus strains isolated from the respiratory tract was assessed over the period 1997-1999 as part of the italian epidemiological observatory survey sponsored by the smith-kline foundation. a standardised method was used to determine the mics of 22 antibiotics against isolates of klebsiella pneumoniae (n=870), escherichia coli (n=684), enterobacter cloacae (n=342), enterobacter aerogenes (n=187) and se ... | 2001 | 11837636 |
| rapid identification of ralstonia eutropha strain ch34 using monoclonal antibodies. | ralstonia eutropha strain ch34 (formerly alcaligenes eutrophus ch34) is an aerobic gram-negative and facultative chemolithotrophic bacteria with plasmid-bound resistance to heavy metals. the presence of ralstonia eutropha strain ch34 is an indication of environmental heavy metals pollution. the major purpose of this work was to produce monoclonal antibodies (mabs) against the metal transport outer membrane proteins. in this way, bacteria outer membranes, grown with or without iron, were purified ... | 2001 | 11839250 |
| emergence of enterobacter aerogenes as a major antibiotic-resistant nosocomial pathogen in belgian hospitals. | objective: to study the epidemiology of enterobacter aerogenes infections in belgian hospitals and determine whether recent trends show an increase in incidence of e. aerogenes infections and antimicrobial resistance. methods: data from the bloodstream infection component of the national surveillance of hospital infections (october 1992 to september 1996 data in 45 hospitals) and from a retrospective study on e. aerogenes clinical isolates (1994 and 1995 data in 41 hospitals) were analyzed. resu ... | 1999 | 11851693 |
| mar operon involved in multidrug resistance of enterobacter aerogenes. | we determined the sequence of the entire marrab operon in enterobacter aerogenes. it is functionally and structurally analogous to the escherichia coli operon. the overexpression of e. aerogenes mara induces a multidrug resistance phenotype in a susceptible strain, demonstrated by a noticeable resistance to various antibiotics, a decrease in immunodetected porins, and active efflux of norfloxacin. | 2002 | 11897595 |
| tem derivative-producing enterobacter aerogenes strains: dissemination of a prevalent clone. | tem-24 (caz-6) extended-spectrum beta-lactamase (esbl) was detected in 1988 in clermont-ferrand, france, in klebsiella pneumoniae (bla(tem-24)) and enterobacter aerogenes (bla(tem-24b)), and since 1994, a tem-24-producing e. aerogenes clonal strain has been observed elsewhere in the country. to determine if the spread of this clonal strain was restricted to tem-24-producing e. aerogenes strains, 84 e. aerogenes strains (non-tem/shv-producing strains, tem-1- or -2-producing strains, and different ... | 2002 | 11897606 |
| epidemiology of extended-spectrum beta-lactamase-producing enterobacter isolates in a spanish hospital during a 12-year period. | fifteen enterobacter clinical isolates (11 enterobacter cloacae isolates, 3 enterobacter aerogenes isolates, and 1 enterobacter gergoviae isolate), representing 0.4% of all enterobacter isolates recovered in our hospital from 1989 to 2000, were suspected of harboring an extended-spectrum beta-lactamase (esbl). these isolates were recovered from 14 different patients. esbls were transferred by conjugation into an escherichia coli recipient strain. pulsed-field gel electrophoresis (pfge) revealed ... | 2002 | 11923338 |
| evolution of the gyrb gene and the molecular phylogeny of enterobacteriaceae: a model molecule for molecular systematic studies. | phylogenetic trees showing the evolutionary relatedness of enterobacteriaceae based upon gyrb and 16s rrna genes were compared. congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. phylogenetic trees based on gyrb sequences appeared to be more reliable at determining relationships among serratia species than trees based on 16s rrna gene sequences. gyrb sequences from serratia spec ... | 2002 | 11931166 |
| prevalence of extended spectrum beta-lactamases among enterobacteriaceae: an italian survey. | the prevalence of extended-spectrum beta-lactamase (esbl) production by consecutive non-repeated isolates of enterobacteriaceae was determined over a 6-month period. a total of 8015 strains were isolated from ten italian laboratories and 509 (6.3%) of these were designated esbl producers from the results of a double-disk synergy test. escherichia coli was the most isolated microrganism, followed by klebsiella pneumoniae and proteus mirabilis. providencia stuartii (28.1%) was the most frequently ... | 2002 | 11932144 |
| risk assessment of hand washing efficacy using literature and experimental data. | this study simulated factors that influence the levels of bacteria on foodservice workers' hands. relevant data were collected from the scientific literature and from laboratory experiments. literature information collected included: initial bacterial counts on hands and water faucet spigots, bacterial population changes during hand washing as effected by soap type, sanitizing agent, drying method, and the presence of rings. experimental data were also collected using enterobacter aerogenes as a ... | 2002 | 11934038 |
| [enterobacteria isolated from cockroaches (periplaneta americana) captured in a brazilian hospital]. | to isolate and identify microorganisms from cockroaches that were captured in a public hospital and to test the antimicrobial susceptibility of these microorganisms. | 2002 | 11939120 |
| wild type variants of penicillinase from klebsiella aerogenes. | | 1968 | 11945261 |
| restoration of electron transport in ultraviolet-irradiated membranes of aerobacter aerogenes. | | 1971 | 11945717 |
| biosynthesis of 4-aminobenzoic acid in aerobacter aerogenes. | | 1971 | 11945911 |
| evidence for induction of the 2,3-butanediol-forming enzymes in aerobacter aerogenes. | | 1968 | 11946262 |
| an intermediate involved in the formation of 4-aminobenzoic acid from chorismic acid in aerobacter aerogenes. | | 1969 | 11947038 |
| purification and some properties of the membrane-bound respiratory nitrate reductase of aerobacter aerogenes. | | 1969 | 11947290 |
| polyphosphate produced in recombinant escherichia coli confers mercury resistance. | an escherichia coli strain was generated by fusion of a mera-deleted broad-spectrum mer operon from pseudomonas k-62 with a bacterial polyphosphate kinase gene (ppk) from klebsiella aerogenes in vector puc119. a large amount of the ppk-specified polyphosphate was identified in the mercury-induced bacterium with the fusion plasmid designated pmkb18 but not in the cells without mercury induction. these results suggest that the synthesis of polyphosphate as well as the expression of the mer genes i ... | 2002 | 11958934 |
| nb2001, a novel antibacterial agent with broad-spectrum activity and enhanced potency against beta-lactamase-producing strains. | enzyme-catalyzed therapeutic activation (ecta) is a novel prodrug strategy to overcome drug resistance resulting from enzyme overexpression. beta-lactamase overexpression is a common mechanism of bacterial resistance to beta-lactam antibiotics. we present here the results for one of the beta-lactamase ecta compounds, nb2001, which consists of the antibacterial agent triclosan in a prodrug form with a cephalosporin scaffold. unlike conventional beta-lactam antibiotics, where hydrolysis of the bet ... | 2002 | 11959554 |
| [post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--ii. gram-negative bacteria]. | as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, penicillins, monobactams, and carbapenems. changes in czop susceptibility for the bacteria were also evaluated with the bacterial resistance ratio calculated with the breakpoint mic. twenty-five species (3,362 strains) of gram-negative bacteria were isolated from the clinica ... | 2002 | 11977920 |
| pyogenic liver abscess -- 20 years' experience. comparison of results of treatment in two periods. | our aim is to give an audit of our experience over the past two decades in the form of a retrospective study. | 2002 | 11981681 |
| cloning and sequencing of enterobacter aerogenes ompc-type osmoporin linked to carbapenem resistance. | using outbreak-related strains of enterobacter aerogenes, we cloned and sequenced ompk39, the structural gene coding for outer membrane protein ompk39. its lack of expression was closely associated with a phenotype exhibiting low-level carbapenem resistance. detailed alignment of the predicted amino acid sequence revealed that ompk39 is a member of the ompc subclass of enterobacterial porins, with the highest degree of homology to klebsiella pneumoniae ompk36. based on a computerized alignment i ... | 2002 | 12008919 |
| [total and fecal coliforms, some enterobacteria staphylococcus sp. and moulds in salads for hot dogs sold in maracay, venezuela]. | fast food sold on the streets are good vehicle to transmit gastrointestinal diseases to consumers because they are highly manipulated. the vegetables are a good media for pathogens growth. raw vegetables salads for hot dog sold in maracay were studied. total and fecal coliforms and staphylococcus sp. were quantified by the most probable number technique, coliforms and staphylococcus isolated were partially identified. also the mould population was quantified and identified. microbial growth was ... | 2001 | 12012562 |
| development of a sensitive and specific enzyme-linked immunosorbent assay for detecting and quantifying cmy-2 and shv beta-lactamases. | polyclonal rabbit antibodies against shv-1 and cmy-2 beta-lactamases were produced and characterized, and enzyme-linked immunosorbent assays (elisas) were developed. immunoblots revealed that the anti-shv-1 antibody recognized shv-1 but did not recognize tem-1, k-1, oxa-1, or any ampc beta-lactamase tested. the anti-cmy-2 antibody detected escherichia coli cmy-2, enterobacter cloacae p99, klebsiella pneumoniae act-1, and the ampc beta-lactamases of enterobacter aerogenes, morganella morganii, an ... | 2002 | 12037047 |
| susceptibility of enterobacteriaceae to beta-lactam agents and fluoroquinolones: a 3-year survey in france. | to assess trends in the susceptibility to beta-lactam agents and to fluoroquinolones of clinically relevant enterobacteriaceae isolated over a 3-year period in 14 french hospital laboratories. | 2002 | 12047412 |
| inhibitors of antibiotic efflux pump in resistant enterobacter aerogenes strains. | enterobacter aerogenes, a nosocomial pathogen, is frequently exhibiting multidrug resistance mechanisms associated with a change in membrane permeability. in clinical isolates, active efflux plays a prominent role in antibiotic resistance. we report here the effect of three unrelated compounds that are able to restore a noticeable antibiotic susceptibility to resistant strains. the targeting of various parameters which contribute to the efficacy of the efflux mechanism, such as energy, flux sele ... | 2002 | 12054665 |
| [yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2000--ii. gram-negative bacteria]. | the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2000 were yearly evaluated and compared with those of other cephems, oxacephems, and carbapenems. thirty-two species 2,697 strains of gram-negative bacteria were isolated from the clinical materials annually collected from january to december, and consisted of moraxella subgenus branhamella catarrhalis (n = 125), escherichia coli (n = 250), citrobacter freu ... | 2002 | 12071094 |
| use of carbon and energy balances in the study of the anaerobic metabolism of enterobacter aerogenes at variable starting glucose concentrations. | the anaerobic metabolism of enterobacter aerogenes was studied in batch culture at increasing initial glucose levels (9.0< s(o) <72 g l(-1)). the ultimate concentrations of fermentation products were utilized to check a metabolic flux analysis based on simple carbon mass and energy balances that promise to be suitable for the study of different fermentation processes, either under aerobic or anaerobic conditions. the stoichiometric coefficients of products collected at increasing starting glucos ... | 2002 | 12111162 |
| three-dimensional quantitative structure-activity relationship and comparative molecular field analysis of dipeptide hydroxamic acid helicobacter pylori urease inhibitors. | a homology model of helicobacter pylori urease was developed by using the crystal structure of urease from klebsiella aerogenes (ec 3.5.1.5) as a template. the acetohydroxamic acid moiety was docked into the active pocket of the enzyme model, followed by relaxation of the complex by use of molecular dynamics. the resulting conformation was used as a template to construct 24 potential dipeptide hydroxamic acid inhibitors with which comparative molecular field analysis (comfa) was performed. the r ... | 2002 | 12121941 |
| the acrab-tolc efflux pump contributes to multidrug resistance in the nosocomial pathogen enterobacter aerogenes. | we identified the genes encoding the acra-acrb-tolc efflux pump in enterobacter aerogenes and constructed acrab and tolc mutants from a multidrug-resistant isolate. both derivatives were more susceptible to antibiotics than the parental strain. sequence analysis and complementation experiments revealed that the multidrug-resistant isolate is an acrr mutant. | 2002 | 12121946 |
| but-1: a new member in the chromosomal inducible class c beta-lactamases family from a clinical isolate of buttiauxella sp. | an atypical enterobacteriaceae strain with a beta-lactam susceptibility pattern of inducible cephalosporinase was isolated in tenon hospital (paris, france) from a patient's skull wound infection. identifications by the api-50che biochemical system and 16s rrna gene sequencing concluded that it was a member of the buttiauxella genus. the bla gene was cloned and sequenced. the deduced translated product was a 383-amino acid protein (but-1) with 75-78% identity with the chromosomal ampc beta-lacta ... | 2002 | 12127496 |
| helicobacter pylori may survive ampicillin treatment in the remnant stomach. | helicobacter pylori (h. pylori) is a gram-negative curved rod-like or spiral bacterium that chronically infects the human gastric mucosa, and is a major risk factor for gastritis, gastric and duodenal ulcer and adenocarcinoma of the stomach. after partial gastrectomy, some patients may have persistent h. pylori infection for five years or more. in this study, we detected three bacteria, i.e., klebsiella pneumoniae, enterobacter aerogenes, and escherichia coli, in the gastric juice of patients wi ... | 2002 | 12139018 |
| dna analysis of nosocomial infection by enterobacter aerogenes in three cases of septicaemia in japan. | ceftazidime-resistant enterobacter aerogenes was isolated from blood cultures of three patients with fever. dna analysis using pulsed-field gel electrophoresis and ribosomal rna gene restriction digest pattern analysis revealed that the strains were clonally similar to each other with a 79.3-96.0% homology. the same strain of e. aerogenes was isolated from a three-way stopcock connected to the indwelling catheter in one of the patients at a concentration of 45 cfu/ml. a similar strain was also i ... | 2002 | 12144802 |
| effectiveness of electrolyzed water as a sanitizer for treating different surfaces. | the effectiveness of electrolyzed (eo) water at killing enterobacter aerogenes and staphylococcus aureus in pure culture was evaluated. one milliliter (approximately 10(9) cfu/ml) of each bacterium was subjected to 9 ml of eo water or control water (eo water containing 10% neutralizing buffer) at room temperature for 30 s. inactivation (reduction of > 9 log10 cfu/ ml) of both pathogens occurred within 30 s after exposure to eo water containing approximately 25 or 50 mg of residual chlorine per l ... | 2002 | 12182480 |
| method for estimation of low outer membrane permeability to beta-lactam antibiotics. | the outer membrane of gram-negative bacteria plays a major role in beta-lactam resistance as it slows down antibiotic entry into the periplasm and therefore acts in synergy with beta-lactamases and efflux systems. up to now, the quantitative estimation of low outer membrane permeability by the method of zimmermann and rosselet was difficult because of the secreted and cell surface-associated beta-lactamases. the method presented here uses the acylation of a highly sensitive periplasmic penicilli ... | 2002 | 12183245 |
| risk factors for infection and molecular typing in patients in the intensive care unit colonized with nosocomial enterobacter aerogenes. | to determine the frequency of colonization by enterobacter aerogenes in patients in the intensive care unit (icu) for more than 48 hours and to evaluate the risk factors for infection in patients colonized by this bacteria. | 2002 | 12186211 |
| prba, a gene coding for an esterase hydrolyzing parabens in enterobacter cloacae and enterobacter gergoviae strains. | the new gene prba encodes an esterase responsible for the hydrolysis of the ester bond of parabens in enterobacter cloacae strain em. this gene is located on the chromosome of strain em and was cloned by several pcr approaches. the prba gene codes for an immature protein of 533 amino acids, the first 31 of which represent a proposed signal peptide yielding a mature protein of a putative molecular mass of 54.6 kda. this enzyme presents analogies with other type b carboxylesterases, mainly of euka ... | 2002 | 12193616 |
| [urinary tract infections due to wide spectrum beta lactamase producing enterobacteriaceae]. | the aim of this study was to specify the characteristics of enterobacterial urinary infections producing wide spectrum beta-lactamase (wsbl) and the management strategies for these patients infected in geriatric wards. | 2002 | 12212511 |
| impact of previous use of antibiotics on development of resistance to extended-spectrum cephalosporins in patients with enterobacter bacteremia. | this retrospective study was conducted to determine the risk factors for resistance to extended-spectrum cephalosporins (escs) and to examine the influence of previous use of an aminoglycoside with an esc on resistance to escs in patients with enterobacter bacteremia from january 1991 through december 2000. a total of 423 episodes of enterobacter bacteremia among 414 patients were documented during the 10-year study period. three hundred thirty-two (78%) isolates were enterobacter cloacae, 72 (1 ... | 2002 | 12226687 |
| chemical modification of specific active site amino acid residues of enterobacter aerogenes glycerol dehydrogenase. | enterobacter aerogenes glycerol dehydrogenase (g1dh ec 1.1.1.6), a tetrameric nad+ specific enzyme catalysing the interconversion of glycerol and dihydroxyacetone, was inactivated on reaction with pyridoxal 5-phosphate (plp) and o-phthalaldehyde (opa). fluorescence spectra of plp-modified, sodium borohydride-reduced g1dh indicated the specific modification of epsilon-amino groups of lysine residues. the extent of inhibition was concentration and time dependent. nad+ and nadh provided complete pr ... | 2002 | 12365461 |
| the first archaeal atp-dependent glucokinase, from the hyperthermophilic crenarchaeon aeropyrum pernix, represents a monomeric, extremely thermophilic rok glucokinase with broad hexose specificity. | an atp-dependent glucokinase of the hyperthermophilic aerobic crenarchaeon aeropyrum pernix was purified 230-fold to homogeneity. the enzyme is a monomeric protein with an apparent molecular mass of about 36 kda. the apparent k(m) values for atp and glucose (at 90 degrees c and ph 6.2) were 0.42 and 0.044 mm, respectively; the apparent v(max) was about 35 u/mg. the enzyme was specific for atp as a phosphoryl donor, but showed a broad spectrum for phosphoryl acceptors: in addition to glucose, whi ... | 2002 | 12374829 |
| surveillance of methicillin-resistant staphylococcus aureus (mrsa) and enterobacteriaceae producing extended-spectrum beta-lactamase (esble) in northern france: a five-year multicentre incidence study. | in order to measure the incidence of methicillin-resistant staphylococcus aureus (mrsa) and of enterobacteriaceae producing extended-spectrum beta-lactamase (esble), and to evaluate the impact of the national guidelines for multidrug-resistant bacteria (mdrb) prevention in hospitals of northern france, a multicentre study was conducted for three months every year starting in 1996, in volunteer hospital laboratories. all clinical specimens positive for mrsa and esble were prospectively surveyed. ... | 2002 | 12398076 |
| the inner-core lipopolysaccharide biosynthetic waae gene: function and genetic distribution among some enterobacteriaceae. | to determine the function of the waae gene in the biosynthesis of the inner-core lps of klebsiella pneumoniae, a waae non-polar mutant has been constructed. data obtained from the comparative chemical analysis of lps samples obtained from the wild-type, the mutant strain and the complemented mutant demonstrated that the waae gene is involved in substitution of alpha-l-glycero-d-manno-heptopyranose i (l,d-heppi) at the o-4 position by a beta-d-glucopyranose (beta-d-glcp) residue. in addition, dna ... | 2002 | 12427940 |
| carbapenem resistance in a clinical isolate of enterobacter aerogenes is associated with decreased expression of ompf and ompc porin analogs. | we investigated the mechanism of imipenem resistance in enterobacter aerogenes strain 810, a clinical isolate from the united states for which the imipenem mic was 16 micro g/ml and the meropenem mic was 8 micro g/ml. an imipenem-susceptible revertant, strain 810-rev, was obtained after multiple passages of the strain on nonselective media. for the revertant, the imipenem mic was </=1 micro g/ml and the meropenem mic was </=0.25 micro g/ml. cefepime mics also decreased from 8 to 1 micro g/ml; ho ... | 2002 | 12435682 |
| repression of glutamate dehydrogenase formation in klebsiella aerogenes requires two binding sites for the nitrogen assimilation control protein, nac. | in klebsiella aerogenes, the gdha gene codes for glutamate dehydrogenase, one of the enzymes responsible for assimilating ammonia into glutamate. expression of a gdhap-lacz transcriptional fusion was strongly repressed by the nitrogen assimilation control protein, nac. this strong repression (>50-fold under conditions of severe nitrogen limitation) required the presence of two separate nac binding sites centered at -89 and +57 relative to the start of gdha transcription. mutants lacking either o ... | 2002 | 12446647 |
| natural antibiotic susceptibility of enterobacter spp., with special reference to enterobacter aerogenes and enterobacter intermedius strains. | the natural susceptibility to 71 antibiotics of 44 strains of enterobacter aerogenes and 12 strains of enterobacter intermedius was examined using a microdilution procedure in isosensitest broth (for all strains) and cation-adjusted mueller hinton broth (for some strains). both species were naturally sensitive or sensitive and intermediate to tetracyclines, all tested aminoglycosides, several penicillins and cephalosporins, carbapenems, aztreonam, quinolones, folate-pathway inhibitors, chloramph ... | 2002 | 12462424 |
| physicochemical characterization and antimicrobial properties of rhamnolipids produced by pseudomonas aeruginosa 47t2 ncbim 40044. | pseudomonas aeruginosa 47t2, grown in submerged culture with waste frying oil as a carbon source, produced a mixture of rhamnolipids with surface activity. up to 11 rhamnolipid homologs (rha-rha-c(8)-c(10); rha-c(10)-c(8)/rha-c(8)-c(10);rha-rha-c(8)-c(12:1); rha-rha-c(10)-c(10); rha-rha-c(10)-c(12:1); rha-c(10)-c(10); rha-rha-c(10)-c(12)/rha-rha-c(12)-c(10); rha-c(10)-c(12:1)/rha-c(12:1)-c(10); rha-rha-c(12:1)-c(12); rha-rha-c(10)-c(14:1); rha-c(10)-c(12)/rha-c(12)-c(10)) were isolated from cult ... | 2003 | 12474254 |
| phylogenetical relationship based on groe genes among phenotypically related enterobacter, pantoea, klebsiella, serratia and erwinia species. | in an attempt to define the phylogenetical relationship among 17 phenotypically related species of genera enterobacter, pantoea, serratia, klebsiella and erwinia, we determined almost all of their groe operon sequences using the polymerase chain reaction direct sequencing method. the number of nucleotide substitutions per site was 0.12+/-0.030. the value was 3.6-fold higher than that of 16s rdna. as a result, we were successful in constructing molecular phylogenetic trees which had a finer resol ... | 1997 | 12501307 |
| ultrasonic enhancement of antibiotic action on several species of bacteria. | the effect of the antibiotics gentamicin, streptomycin, kanamycin, tetracycline, and ampicillin on planktonic cultures of enterobacter aerogenes, serratia marcescens, salmonella derby, streptococcus mitis, and staphylococcus epidermidis with and without an application of 70 khz ultrasound was studied. the ultrasound was applied at levels that had no inhibitory effect on planktonic cultures of bacteria. measurements of viability at, above, and below the minimum inhibitory concentration of the abo ... | 1998 | 12501423 |
| isolation of a negative control mutant of the nitrogen assimilation control protein, nac, in klebsiella aerogenes. | a negative control mutant of the nitrogen assimilation control protein, nac, has been isolated. mutants with the leucine at position 111 changed to a nonhydrophobic residue activate transcription from hut and ure promoters, but fail to repress gdha expression. this failure does not result from failure to bind to either of the two sites required for gdha repression, but the binding at those sites is altered in the mutant. it appears that the nac negative control mutants fail to form the complex s ... | 2003 | 12511519 |
| evaluation of antibacterial efficacy of drugs for urinary tract infections by genotyping based on pulsed-field gel electrophoresis (pfge). | we attempted to determine whether the same bacterium isolated from patients with urinary tract infections (uti) before and after treatment with antimicrobial agents was of the same strain ("persisted") or of a different strain ("changed"). furthermore, to verify the effectiveness of antimicrobial agents for uti, we investigated whether bacterial strains could be classified as persisted or changed based on their electrophoretic patterns in pulsed-field gel electrophoresis (pfge). we examined eigh ... | 2002 | 12525898 |
| [purification and properties of chitinase from enterobacter aerogenes]. | a bacterium producing chitinase was isolated from the dead body of gymephorap ruoergensis. a chitinase was isolated from the culture of e. aerogenes and purified by means of ammonium sulfate precipitation, deae-cellulose column chromatography, and sephadex g-100 column gel filtration. the purified chitinase showed homogeneity on the native polyacrylamide gel electrophoresis. its molecular weight was estimated to be about 42.5 kd by sds-page. the optimum ph and temperature for hydrolysis of chiti ... | 2001 | 12549194 |
| the comparative effect of novel pelargonium essential oils and their corresponding hydrosols as antimicrobial agents in a model food system. | essential oils and their corresponding hydrosols, obtained after distillation of various scented pelargonium (geraniaceae) leaves were assessed for their antimicrobial activity in a model food system. both the essential oils and hydrosols were used at 1000 ppm in broccoli soup, previously inoculated with enterobacter aerogenes (at 10(5) cfu g(-1)) and staphylococcus aureus (at 10(4) cfu g(-1)). the results showed a complete inhibition of s. aureus in the broccoli soup by the essential oils of 's ... | 2003 | 12557249 |
| imipenem and expression of multidrug efflux pump in enterobacter aerogenes. | imipenem is often used to treat intensive care unit patients infected by enterobacter aerogenes, but it is leading to an increasing number of antibiotic resistant strains. clinical isolates and imipenem resistant variants presented a high level of resistance to beta-lactam antibiotic group and to chemically unrelated drugs. we report here that imipenem selects strains which contain active efflux pumps ejecting various unrelated antibiotics including quinolones, tetracycline, and chloramphenicol. ... | 2003 | 12589810 |
| effect of specific oxygen uptake rate on enterobacter aerogenes energetics: carbon and reduction degree balances in batch cultivations. | the effect of oxygen availability on the metabolism of enterobacter aerogenes ncimb 10102 was studied through batch fermentations of glucose performed increasing the specific oxygen uptake rate up to 72.7 mmol(o2) c-mol(dw) (-1) x h(-1). the final concentrations of fermentation products of this biosystem (2,3-butanediol, hydrogen, acetoin, formate, acetate, carbon dioxide, ethanol, lactate, succinate, and biomass) were utilized to check the use of simple carbon mass and reduction degree balances ... | 2003 | 12599264 |
| sequencing of 16s rdna of klebsiella: taxonomic relations within the genus and to other enterobacteriaceae. | the 16s rdnas of 20 strains of klebsiella were sequenced and used for construction of a phylogenetic tree together with already published enterobacteriaceae 16s rdna sequences. the taxonomy within the klebsiella genus, as reflected by the 16s rdna tree, was in agreement with existing dna-dna hybridisation and numerical taxonomy data, indicating that for klebsiella, 16s rdna sequencing is a valid method for identification and taxonomical purposes. five closely related clusters were found in the k ... | 2003 | 12635932 |
| overexpression and purification of the three components of the enterobacter aerogenes acra-acrb-tolc multidrug efflux pump. | the tripartite acra-acrb-tolc system is the major efflux pump of the nosocomial pathogen enterobacter aerogenes. acra is a trimeric periplasmic lipoprotein anchored in the inner membrane, acrb is an inner membrane transporter and tolc is a trimeric outer membrane channel. in order to reconstitute the acra-acrb-tolc system of e. aerogenes in artificial membranes, we overexpressed and purified the three proteins. the e. aerogenes acra, acrb and tolc open reading frames were individually inserted i ... | 2003 | 12651015 |
| extended spectrum beta-lactamase mediated resistance in urinary tract isolates of family enterobacteriaceae. | extended spectrum beta-lactamases (esbl) hydrolyse expanded spectrum cephalosporins like ceftazidime, cefotaxime and monobactam aztreonam. esbl producing bacteria may not be detectable in the routine disk diffusion susceptibility tests leading to inappropriate use of antibiotics and treatment failures. as information on esbl producers causing urinary tract infection (uti) is not available from our country, the occurrence of esbl producing strains causing uti was studied as also the differences b ... | 2002 | 12674828 |
| characterization of clinical isolates of enterobacteriaceae from italy by the bd phoenix extended-spectrum beta-lactamase detection method. | production of extended-spectrum beta-lactamases (esbls) is an important mechanism of beta-lactam resistance in enterobacteriaceae: identification of esbls based on phenotypic tests is the strategy most commonly used in clinical microbiology laboratories. the phoenix esbl test (bd diagnostic systems, sparks, md.) is a recently developed automated system for detection of esbl-producing gram-negative bacteria. an algorithm based on phenotypic responses to a panel of cephalosporins (ceftazidime plus ... | 2003 | 12682131 |
| potential for detection of microorganisms and heavy metals in potable water using electronic nose technology. | studies have been carried out to determine the potential for the detection of different microbial species (enterobacter aerogenes, escherichia coli, pseudomonas aeruginosa), alone and in the presence of low concentrations of different heavy metals (as, cd, pb and zn) in bottled, reverse osmosis (ro) and tap water, using an electronic nose. studies show that it is possible to discriminate control water samples from water contaminated with 0.5 ppm of a mixture of metals. the presence of heavy meta ... | 2003 | 12706588 |
| modification of outer membrane protein profile and evidence suggesting an active drug pump in enterobacter aerogenes clinical strains. | two clinical strains of enterobacter aerogenes that exhibited phenotypes of multiresistance to beta-lactam antibiotics, fluoroquinolones, chloramphenicol, tetracycline, and kanamycin were investigated. both strains showed a porin pattern different from that of a susceptible strain, with a drastic reduction in the amount of the major porin but with an apparently conserved normal structure (size and immunogenicity), together with overproduction of two known outer membrane proteins, ompx and lamb. ... | 2003 | 12709321 |
| surfactant proteins a and d inhibit the growth of gram-negative bacteria by increasing membrane permeability. | the pulmonary collectins, surfactant proteins a (sp-a) and d (sp-d), have been reported to bind lipopolysaccharide (lps), opsonize microorganisms, and enhance the clearance of lung pathogens. in this study, we examined the effect of sp-a and sp-d on the growth and viability of gram-negative bacteria. the pulmonary clearance of escherichia coli k12 was reduced in sp-a-null mice and was increased in sp-d-overexpressing mice, compared with strain-matched wild-type controls. purified sp-a and sp-d i ... | 2003 | 12750409 |
| correlation between bacterial haemoglobin gene (vgb) and aeration: their effect on the growth and alpha-amylase activity in transformed enterobacter aerogenes. | to evaluate the effects of bacterial haemoglobin on bacterial growth and alpha-amylase formation under different aeration conditions. | 2003 | 12752814 |
| biosynthesis of pyridoxine in saccharomyces cerevisiae--origin of the pyridoxine nitrogen atom differs under anaerobic and aerobic conditions. | the amide nitrogen atom of glutamine is incorporated into pyridoxine in four eukaryotes (i.e., emericella nidulans, mucor racemosus, neurospora crassa and saccharomyces cerevisiae) and two prokaryotes (i.e., staphylococcus aureus and bacillus subtilis). however, in the prokaryotes pseudomonas putida, enterobacter aerogenes and escherichia coli, it is the nitrogen atom of glutamate that is incorporated into pyridoxine (j nutr sci vitaminol (2000) 46, 55-57). as these results were from experiments ... | 2002 | 12775110 |
| the escherichia fergusonii iucabcd iuta genes are located within a larger chromosomal region similar to pathogenicity islands. | three strains of escherichia fergusonii (ef873, ef1496, ef939) of 50 strains tested produced the hydroxamate siderophore aerobactin. screening of a cosmid library of the strain ef873 chromosomal dna (in aerobactin nonproducing escherichia coli vcs257) for aerobactin production identified iucabcd and iuta gene orthologues. the predicted iucabcd and iuta proteins showed 59-65% identity to the corresponding proteins of shigella flexneri and e. coli. aerobactin molecules synthesized by e. fergusonii ... | 2003 | 12800494 |
| persistent silver disinfectant for the environmental control of pathogenic bacteria. | contaminated surfaces can act as a reservoir for pathogenic microorganisms and potentially exacerbate the risk of infection. surface disinfection and decontamination provide temporary amelioration against bacterial colonization. disinfected surfaces eventually become contaminated, thus, mitigating the benefit of the initial disinfection. it is hypothesized that to improve on the current state of the art, a disinfectant should not only immediately disinfect a surface but also provide persistent a ... | 2003 | 12806357 |
| the beta-glucoside genes of klebsiella aerogenes: conservation and divergence in relation to the cryptic bgl genes of escherichia coli. | the ability to metabolize aromatic beta-glucosides such as salicin and arbutin varies among members of the enterobacteriaceae. the ability of escherichia coli to degrade salicin and arbutin appears to be cryptic, subject to activation of the bgl genes, whereas many members of the klebsiella genus can metabolize these sugars. we have examined the genetic basis for beta-glucoside utilization in klebsiella aerogenes. the klebsiella equivalents of bglg, bglb and bglr have been cloned using the genom ... | 2003 | 12829297 |
| comparison of four antimicrobial susceptibility testing methods to determine the in vitro activities of piperacillin and piperacillin-tazobactam against clinical isolates of enterobacteriaceae and pseudomonas aeruginosa. | susceptibility to piperacillin was similar to that to piperacillin-tazobactam (<1% difference) for 6,938 isolates of enterobacter aerogenes and 13,954 isolates of enterobacter cloacae tested using a vitek system; for the same species, in contrast, susceptibility rates to piperacillin-tazobactam were 5.9 to 13.9% higher than to piperacillin using disk diffusion, microscan, and vitek 2 testing. unprecedented phenotypes (piperacillin susceptible and piperacillin-tazobactam intermediate; piperacilli ... | 2003 | 12843088 |
| crayfish broth for bacterial culture. | to formulate a new growth medium for bacteriological studies. | 2000 | 12858918 |
| comparative in vitro activity of three fluoroquinolones against clinical isolates by e test. | the in vitro susceptibility of levofloxacin, ciprofloxacin and moxifloxacin against several gram-positive and gram-negative clinical isolates was tested by e test. we found that the mic(50) and mic(90) values against all members of the enterobacteriaceae family except serratia were <0.5 mg/l for ciprofloxacin and levofloxacin (mic range 0.006-32 mg/l) based on the in vitro susceptibility data. the susceptibility rates for ciprofloxacin and levofloxacin were more than 85% for escherichia coli, ci ... | 2003 | 12886053 |
| genetic engineering of enterobacter aerogenes with the vitreoscilla hemoglobin gene: cell growth, survival, and antioxidant enzyme status under oxidative stress. | hemoglobins in unicellular organisms, like the one here in the bacterium vitreoscilla, have greater chemical reactivity than their homologues in multicellular organisms. they can catalyze redox reactions and may protect cells against oxidative stress. the ability of vitreoscilla hemoglobin to complement deficiencies of terminal cytochrome oxidases in escherichia coli also suggests that this hemoglobin can receive electrons during respiration. in this study, a recombinant strain of enterobacter a ... | 2003 | 12892849 |
| study on highly diastereoselective synthesis of (2'r)-2'-deoxy[2'-2h]guanosine. | to develop an efficient method for the synthesis of a highly diasteroselective (2'r)-2'-deoxy[2'-2h]guanosine (1), studies of organic chemical conversion from 2'-bromo-2'-deoxy-n2-isobutyryl-3',5'-o-tipds-guanosine (2) to 1 and a biological transdeoxyribofuranosylation of (2'r > 98% de)-2'-deoxy[2'-2h]uridine (4) were carried out. as the results, a highly diastereoselective synthesis of 1 was achieved by a biological transdeoxyribofuranosylation between 2,6-diaminopurine and 4 by the use of ente ... | 2000 | 12903250 |
| modification of the double-disk test for detection of enterobacteriaceae producing extended-spectrum and ampc beta-lactamases. | detection of extended-spectrum beta-lactamases (esbls) in ampc-producing enterobacteriaceae is problematic. a modification of the double-disk test (mddt) has been developed for successful detection of esbls in gram-negative bacilli producing well-characterized beta-lactamases as well as 212 clinical isolates of enterobacter cloacae, enterobacter aerogenes, serratia marcescens, and citrobacter freundii. mddt accurately differentiated between esbl producers and derepressed chromosomal ampc mutants ... | 2003 | 12904422 |
| comparison of microbial adherence to antiseptic and antibiotic central venous catheters using a novel agar subcutaneous infection model. | an agar subcutaneous infection model (agar model), which simulates the rat subcutaneous infection model (rat model), was developed to assess the ability of antimicrobial catheters to resist microbial colonization. the catheters were implanted in the agar and rat models and the insertion sites were infected immediately or on day 7, 14 or 21 post-implantation. the catheters implanted in the agar model were transferred to fresh media one day before infection on day 7, 14 or 21. the efficacy of chlo ... | 2003 | 12917250 |
| molecular epidemiology of enterobacteriaceae producing extended-spectrum beta-lactamase in a french university-affiliated hospital. | we conducted a retrospective study to investigate the epidemiology of enterobacteriaceae producing extended-spectrum beta-lactamase (esble) in our hospital. we determined the occurrence of extended-spectrum beta-lactamase (esbl) in enterobacteriaceae over a 2-year period. we also characterised esbls by isoelectric focusing (ief) and investigated the epidemiological relatedness of eblse by pulsed field gel electrophoresis (pfge). during this period, 70 patients were colonised/infected with one or ... | 2003 | 12927952 |
| extended-spectrum beta-lactamases in enterobacteriaceae in buenos aires, argentina, public hospitals. | resistance to extended-spectrum cephalosporins is often associated with plasmid encoded extended spectrum beta-lactamases (esbl). in order to evaluate the prevalence and diversity of esbls in enterobacteria in our city, a 1-month-period survey was carried out from april to may 2000. extended-spectrum-cephalosporin-resistant strains, isolated from inpatient clinical specimens other than stools, were collected among 17 participating hospitals. from a total of 427 enterobacterial strains that were ... | 2003 | 12936986 |
| outbreak of tem-24-producing enterobacter aerogenes in a spanish hospital. | organisms encoding multidrug resistance genes are becoming increasingly prevalent. during a 2-month period (december, 2000, to january, 2001), 83 consecutive isolates of enterobacter spp. were collected in our microbiology department. antibiotic susceptibility was determined using the vitek ii automatic system. we selected strains with decreased susceptibility to extended-spectrum cephalosporins. the double-disk potentiation test was positive in 10 of these strains, indicating the presence of ex ... | 2003 | 12959409 |
| characterization of blacmy-10 a novel, plasmid-encoded ampc-type beta-lactamase gene in a clinical isolate of enterobacter aerogenes. | we report the description of a novel plasmid-encoded ampc beta-lactamase gene (blacmy-10) from enterobacter aerogenes k9911729 that was isolated from a patient suffering from pneumonia in south korea. | 2003 | 12969288 |
| evaluation of oxoid combination discs for detection of extended-spectrum beta-lactamases. | we evaluated the reliability of cefpirome/clavulanate (cd04) compared with ceftazidime/clavulanate (cd02) and cefotaxime/clavulanate (cd03) oxoid combination discs for the detection of extended-spectrum beta-lactamases (esbl) in several enterobacteriaceae isolates, including enterobacter spp. | 2003 | 12972449 |