| cost-effectiveness of novel system of mosquito surveillance and control, brazil. | of all countries in the western hemisphere, brazil has the highest economic losses caused by dengue fever. we evaluated the cost-effectiveness of a novel system of vector surveillance and control, monitoramento inteligente da dengue (intelligent dengue monitoring system [mid]), which was implemented in 21 cities in minas gerais, brazil. traps for adult female mosquitoes were spaced at 300-m intervals throughout each city. in cities that used mid, vector control was conducted specifically at high ... | 0 | 23628282 |
| talen-based gene disruption in the dengue vector aedes aegypti. | in addition to its role as the primary vector for dengue viruses, aedes aegypti has a long history as a genetic model organism for other bloodfeeding mosquitoes, due to its ease of colonization, maintenance and reproductive productivity. though its genome has been sequenced, functional characterization of many ae. aegypti genes, pathways and behaviors has been slow. tale nucleases (talens) have been used with great success in a number of organisms to generate site-specific dna lesions. we evalua ... | 2013 | 23555893 |
| oral ingestion of transgenic ridl ae. aegypti larvae has no negative effect on two predator toxorhynchites species. | dengue is the most important mosquito-borne viral disease. no specific treatment or vaccine is currently available; traditional vector control methods can rarely achieve adequate control. recently, the ridl (release of insect carrying dominant lethality) approach has been developed, based on the sterile insect technique, in which genetically engineered 'sterile' homozygous ridl male insects are released to mate wild females; the offspring inherit a copy of the ridl construct and die. a ridl stra ... | 2013 | 23527029 |
| high efficiency of temperate aedes albopictus to transmit chikungunya and dengue viruses in the southeast of france. | since 2005, cases of chikungunya (chik) were caused by an unusual vector, aedes albopictus. this mosquito, present in europe since 1979, has gained importance since its involvement in the first chik outbreak in italy in 2007. the species is capable of transmitting experimentally 26 arboviruses. however, the vectorial status of its temperate populations has remained little investigated. in 2010, autochthonous cases of chik and dengue (den) were reported in southeastern france. we evaluated the po ... | 2013 | 23527259 |
| a model for the spatial transmission of dengue with daily movement between villages and a city. | dengue is a re-emergent vector-borne disease affecting large portions of the world's population living in the tropics and subtropics. the virus is transmitted through the bites of female aedes aegypti mosquitoes, and it is widely believed that these bites occur primarily in the daytime. the transmission of dengue is a complicated process, and one of the main sources of this complexity is due to the movement of people, e.g. between home and their places of work. hence, the mechanics of disease pr ... | 2013 | 23475426 |
| functional genetic characterization of salivary gland development in aedes aegypti. | despite the devastating global impact of mosquito-borne illnesses on human health, very little is known about mosquito developmental biology. in this investigation, functional genetic analysis of embryonic salivary gland development was performed in aedes aegypti, the dengue and yellow fever vector and an emerging model for vector mosquito development. although embryonic salivary gland development has been well studied in drosophila melanogaster, little is known about this process in mosquitoes ... | 2013 | 23497573 |
| bacillus thuringiensis var. israelensis misting for control of aedes in cryptic ground containers in north queensland, australia. | in australia, dengue is not endemic, although the vector mosquito aedes aegypti is established in far north queensland (fnq). aedes albopictus has recently invaded the torres strait region, but is not established on mainland australia. to maintain dengue-free, public health departments in fnq closely monitor introduced dengue infections and confine outbreaks through rigorous vector control responses. to safeguard mainland australia from ae. albopictus establishment, pre-emptive strategies are re ... | 2013 | 23358637 |
| skeeter buster: a stochastic, spatially explicit modeling tool for studying aedes aegypti population replacement and population suppression strategies. | dengue is the most important mosquito-borne viral disease affecting humans. the only prevention measure currently available is the control of its vectors, primarily aedes aegypti. recent advances in genetic engineering have opened the possibility for a new range of control strategies based on genetically modified mosquitoes. assessing the potential efficacy of genetic (and conventional) strategies requires the availability of modeling tools that accurately describe the dynamics and genetics of a ... | 2009 | 19721700 |
| effects of inbreeding and genetic modification on aedes aegypti larval competition and adult energy reserves. | genetic modification of mosquitoes offers a promising strategy for the prevention and control of mosquito-borne diseases. for such a strategy to be effective, it is critically important that engineered strains are competitive enough to serve their intended function in population replacement or reduction of wild mosquitoes in nature. thus far, fitness evaluations of genetically modified strains have not addressed the effects of competition among the aquatic stages and its consequences for adult f ... | 2010 | 20925917 |
| aegepuci: a database of gene expression in the dengue vector mosquito, aedes aegypti. | aedes aegypti is the principal vector of dengue and yellow fever viruses. the availability of the sequenced and annotated genome enables genome-wide analyses of gene expression in this mosquito. the large amount of data resulting from these analyses requires efficient cataloguing before it becomes useful as the basis for new insights into gene expression patterns and studies of the underlying molecular mechanisms for generating these patterns. | 2010 | 20920356 |
| whole-mount in situ hybridization for analysis of gene expression during aedes aegypti development. | blood-feeding mosquitoes, including the dengue and yellow fever vector aedes aegypti, transmit many of the world's deadliest diseases. such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. this protocol for whole-mo ... | 2010 | 20889706 |
| oviposition deterrent activity from the ethanolic extract of pongamia pinnata, coleus forskohlii, and datura stramonium leaves against aedes aegypti and culex quinquefaciatus. | mosquitoes are responsible for spread of many diseases than any other group of arthropods. diseases such as malaria, filariasis, dengue hemorrhagic fever (dhf), and chikunguinya are real threat to mankind. in the present study, ethanolic extracts of leaves of pongamia pinnata, coleus forskohlii, and datura stramonium were evaluated for oviposition deterrent activity against aedes aegypti and culex quinquefasciatus. the oviposition deterrent tests of ethanolic extract of pongamia pinnata, coleus ... | 0 | 21120036 |
| immunohistochemical analysis of protein expression during aedes aegypti development. | blood-feeding mosquitoes, including the dengue and yellow fever vector aedes aegypti, transmit many of the world's deadliest diseases. such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and gene products that regulate development are of particular interest. this protocol for ... | 2010 | 20889707 |
| fixation and preparation of developing tissues from aedes aegypti. | blood-feeding mosquitoes, including the dengue and yellow fever vector aedes aegypti, transmit many of the world's deadliest diseases. such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. this protocol describes a ... | 2010 | 20889705 |
| functional analysis of genes in aedes aegypti embryos. | blood-feeding mosquitoes, including the dengue and yellow fever vector aedes aegypti, transmit many of the world's deadliest diseases. such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. in recent years, rna inter ... | 2010 | 20889708 |
| field evaluation of pyriproxyfen and spinosad mixture for the control of insecticide resistant aedes aegypti in martinique (french west indies). | the resistance of ae. aegypti to insecticides is already widespread and continues to develop. it represents a serious problem for programmes aimed at the control and prevention of dengue in tropical countries. in the light of this problem measures to control ae. aegypti are being orientated towards how best to use existing insecticides, notably by combining those that have different modes of action. | 2010 | 20843383 |
| new protective battle-dress impregnated against mosquito vector bites. | mixing repellent and organophosphate (op) insecticides to better control pyrethroid resistant mosquito vectors is a promising strategy developed for bed net impregnation. here, we investigated the opportunity to adapt this strategy to personal protection in the form of impregnated clothes. | 2010 | 20809969 |
| a simple colorimetric assay for specific detection of glutathione-s transferase activity associated with ddt resistance in mosquitoes. | insecticide-based methods represent the most effective means of blocking the transmission of vector borne diseases. however, insecticide resistance poses a serious threat and there is a need for tools, such as diagnostic tests for resistance detection, that will improve the sustainability of control interventions. the development of such tools for metabolism-based resistance in mosquito vectors lags behind those for target site resistance mutations. | 2010 | 20824165 |
| modeling the impact on virus transmission of wolbachia-mediated blocking of dengue virus infection of aedes aegypti. | dengue is the most common arboviral infection of humans and is a public health burden in more than 100 countries. aedes aegypti mosquitoes stably infected with strains of the intracellular bacterium wolbachia are resistant to dengue virus (denv) infection and are being tested in field trials. to mimic field conditions, we experimentally assessed the vector competence of a. aegypti carrying the wolbachia strains wmel and wmelpop after challenge with viremic blood from dengue patients. we found th ... | 0 | 25787763 |
| silencing of end-joining repair for efficient site-specific gene insertion after talen/crispr mutagenesis in aedes aegypti. | conventional control strategies for mosquito-borne pathogens such as malaria and dengue are now being complemented by the development of transgenic mosquito strains reprogrammed to generate beneficial phenotypes such as conditional sterility or pathogen resistance. the widespread success of site-specific nucleases such as transcription activator-like effector nucleases (talens) and clustered regularly interspaced short palindromic repeats (crispr)/cas9 in model organisms also suggests that repro ... | 2015 | 25775608 |
| risk factors for the presence of chikungunya and dengue vectors (aedes aegypti and aedes albopictus), their altitudinal distribution and climatic determinants of their abundance in central nepal. | the presence of the recently introduced primary dengue virus vector mosquito aedes aegypti in nepal, in association with the likely indigenous secondary vector aedes albopictus, raises public health concerns. chikungunya fever cases have also been reported in nepal, and the virus causing this disease is also transmitted by these mosquito species. here we report the results of a study on the risk factors for the presence of chikungunya and dengue virus vectors, their elevational ceiling of distri ... | 2015 | 25774518 |
| evaluation of simultaneous transmission of chikungunya virus and dengue virus type 2 in infected aedes aegypti and aedes albopictus (diptera: culicidae). | the simultaneous transmission of chikungunya virus (chikv) and dengue viruses (denv) has been a major public health concern because of their sympatric distribution and shared mosquito vectors. groups of aedes aegypti (l.) and aedes albopictus (skuse) were orally infected with 1.5 × 10(5) pfu/ml of chikv and 3.2 × 10(6) ffu/ml of denv-2 simultaneously or separately in inverse orders and evaluated for dissemination and transmission by qrt-pcr. simultaneous dissemination of both viruses was detecte ... | 2015 | 26334820 |
| evaluation of household bleach as an ovicide for the control of aedes aegypti. | accumulations of dormant eggs in container habitats allow aedes aegypti populations to survive harsh environmental conditions and may frustrate control interventions directed at larval and adult life stages. while sodium hypochlorite solutions (naocl) have long been recognized as ovicides for use against dengue vectors, the susceptibility of eggs to spray applications has not been robustly evaluated on substrate materials representative of the most frequently utilized artificial container habita ... | 0 | 25843179 |
| mechanistic basis for morphological damage induced by essential oil from brazilian pepper tree, schinus terebinthifolia, on larvae of stegomyia aegypti, the dengue vector. | dengue has become the subject of public health programs worldwide. the lack of a vaccine and the high environmental risk of synthetic insecticides, arouse the interest in natural products against this vector. this study aimed to determine the chemical composition of the essential oil of ripe fruits and seeds of schinus terebinthifolia raddi; to evaluate the essential oil effect on mortality of stegomyia aegypti (linnaeus, 1792) larvae; and to characterize the structural damage suffered by larvae ... | 2015 | 25886180 |
| evolution of dengue disease and entomological monitoring in santa cruz, bolivia 2002 - 2008. | in the context of a rapid increase of dengue cases in the americas, a monitoring system based on systematic serological control (igm) of patients consulting for suspected dengue was developed in bolivia at the end of the 1990s. in the most affected city of santa cruz, this system was complemented by an entomological surveillance program based on periodical search for immature stages of aedes aegypti in dwelling water-holding containers. here, we analyze these data and describe dengue patterns ov ... | 2015 | 25706631 |
| rhamnolipids: solution against aedes aegypti? | aedes aegypti mosquitoes are the primary transmitters of dengue fever, urban yellow fever, and chikungunya viruses. this mosquito has developed resistance to the insecticides currently used to control their populations. these chemical insecticides are harmful to the environment and can have negative effects on human health. rhamnolipids are environmentally compatible biological surfactants, but their insecticidal activity has not been extensively studied. the present study evaluated the potentia ... | 2015 | 25762986 |
| silencing the buzz: a new approach to population suppression of mosquitoes by feeding larvae double-stranded rnas. | mosquito-borne diseases threaten over half the world's human population, making the need for environmentally-safe mosquito population control tools critical. the sterile insect technique (sit) is a biological control method that can reduce pest insect populations by releasing a large number of sterile males to compete with wild males for female mates to reduce the number of progeny produced. typically, males are sterilized using radiation, but such methods can reduce their mating competitiveness ... | 2015 | 25880645 |
| surveillance of aedes aegypti: comparison of house index with four alternative traps. | the mosquito aedes aegypti, vector of dengue, chikungunya and yellow fever viruses, is an important target of vector control programs in tropical countries. most mosquito surveillance programs are still based on the traditional household larval surveys, despite the availability of new trapping devices. we report the results of a multicentric entomological survey using four types of traps, besides the larval survey, to compare the entomological indices generated by these different surveillance to ... | 2015 | 25668559 |
| spatial variations in dengue transmission in schools in thailand. | dengue is an important neglected tropical disease, with more than half of the world's population living in dengue endemic areas. good understanding of dengue transmission sites is a critical factor to implement effective vector control measures. | 2016 | 27669170 |
| the correlation between dengue incidence and diurnal ranges of temperature of colombo district, sri lanka 2005-2014. | meteorological factors affect dengue transmission. mechanisms of the way in which different diurnal temperatures, ranging around different mean temperatures, influence dengue transmission were published after 2011. | 2016 | 27566717 |
| quantitative trait loci mapping of genome regions controlling permethrin resistance in the mosquito aedes aegypti. | the mosquito aedes aegypti is the principal vector of dengue and yellow fever flaviviruses. permethrin is an insecticide used to suppress ae. aegypti adult populations but metabolic and target site resistance to pyrethroids has evolved in many locations worldwide. quantitative trait loci (qtl) controlling permethrin survival in ae. aegypti were mapped in an f(3) advanced intercross line. parents came from a collection of mosquitoes from isla mujeres, méxico, that had been selected for permethrin ... | 2008 | 18723882 |
| large indoor cage study of the suppression of stable aedes aegypti populations by the release of thiotepa-sterilised males. | the sterile insect technique (sit) is a promising pest control method in terms of efficacy and environmental compatibility. in this study, we determined the efficacy of thiotepa-sterilised males in reducing the target aedes aegypti populations. treated male pupae were released weekly into large laboratory cages at a constant ratio of either 5:1 or 2:1 sterile-to-fertile males. a two-to-one release ratio reduced the hatch rate of eggs laid in the cage by approximately a third and reduced the adul ... | 2014 | 24863972 |
| designing a community engagement framework for a new dengue control method: a case study from central vietnam. | the wolbachia strategy aims to manipulate mosquito populations to make them incapable of transmitting dengue viruses between people. to test its efficacy, this strategy requires field trials. public consultation and engagement are recognized as critical to the future success of these programs, but questions remain regarding how to proceed. this paper reports on a case study where social research was used to design a community engagement framework for a new dengue control method, at a potential r ... | 2014 | 24853391 |
| spatial distribution of the risk of dengue and the entomological indicators in sumaré, state of são paulo, brazil. | dengue fever is a major public health problem worldwide, caused by any of four virus (denv-1, denv-2, denv-3 and denv-4; flaviviridae: flavivirus), transmitted by aedes aegypti mosquito. reducing the levels of infestation by a. aegypti is one of the few current strategies to control dengue fever. entomological indicators are used by dengue national control program to measure the infestation of a. aegypti, but little is known about predictive power of these indicators to measure dengue risk. in t ... | 2014 | 24831806 |
| geometric morphometrics of nine field isolates of aedes aegypti with different resistance levels to lambda-cyhalothrin and relative fitness of one artificially selected for resistance. | aedes aegypti, a mosquito closely associated with humans, is the principal vector of dengue virus which currently infects about 400 million people worldwide. because there is no way to prevent infection, public health policies focus on vector control; but insecticide-resistance threatens them. however, most insecticide-resistant mosquito populations exhibit fitness costs in absence of insecticides, although these costs vary. research on components of fitness that vary with insecticide-resistance ... | 2014 | 24801598 |
| survival and swimming behavior of insecticide-exposed larvae and pupae of the yellow fever mosquito aedes aegypti. | the yellow fever mosquito aedes aegypti is essentially a container-inhabiting species that is closely associated with urban areas. this species is a vector of human pathogens, including dengue and yellow fever viruses, and its control is of paramount importance for disease prevention. insecticide use against mosquito juvenile stages (i.e. larvae and pupae) is growing in importance, particularly due to the ever-growing problems of resistance to adult-targeted insecticides and human safety concern ... | 2014 | 24761789 |
| phylogeography and spatio-temporal genetic variation of aedes aegypti (diptera: culicidae) populations in the florida keys. | aedes aegypti (l.) is the principal mosquito vector of dengue fever, the second-most deadly vector-borne disease in the world. in ae. aegypti and other arthropod disease vectors, genetic markers can be used to inform us about processes relevant to disease spread, such as movement of the vectors across space and the temporal stability of vector populations. in late 2009, 27 locally acquired cases of dengue fever were reported in key west, fl. the last dengue outbreak in the region occurred in 193 ... | 0 | 23540116 |
| an integrated linkage, chromosome, and genome map for the yellow fever mosquito aedes aegypti. | aedes aegypti, the yellow fever mosquito, is an efficient vector of arboviruses and a convenient model system for laboratory research. extensive linkage mapping of morphological and molecular markers localized a number of quantitative trait loci (qtls) related to the mosquito's ability to transmit various pathogens. however, linking the qtls to ae. aegypti chromosomes and genomic sequences has been challenging because of the poor quality of polytene chromosomes and the highly fragmented genome a ... | 2013 | 23459230 |
| transinfected wolbachia have minimal effects on male reproductive success in aedes aegypti. | wolbachia are maternally inherited endosymbiotic bacteria that manipulate the reproductive success of their insect hosts. uninfected females that mate with wolbachia infected males do not reproduce due to cytoplasmic incompatibility (ci). ci results in the increased frequency of wolbachia-infected individuals in populations. recently, two wolbachia strains, the benign wmel and virulent wmelpop have been artificially transinfected into the primary vector of dengue virus, the mosquito ae. aegypti ... | 2013 | 23399027 |
| evolution of resistance to satyrization through reproductive character displacement in populations of invasive dengue vectors. | recently, the highly invasive asian tiger mosquito, aedes albopictus, rapidly displaced resident populations of the yellow fever mosquito, aedes aegypti in the southeastern united states and in bermuda. although multiple mechanisms of competitive displacement have been hypothesized, recent evidence of cross-insemination between these species in nature and the sterilizing effects of male accessory gland products asymmetrically favoring a. albopictus in interspecific matings support a role for sat ... | 2013 | 23359710 |
| field cage studies and progressive evaluation of genetically-engineered mosquitoes. | a genetically-engineered strain of the dengue mosquito vector aedes aegypti, designated ox3604c, was evaluated in large outdoor cage trials for its potential to improve dengue prevention efforts by inducing population suppression. ox3604c is engineered with a repressible genetic construct that causes a female-specific flightless phenotype. wild-type females that mate with homozygous ox3604c males will not produce reproductive female offspring. weekly introductions of ox3604c males eliminated all ... | 2013 | 23350003 |
| associative learning in the dengue vector mosquito, aedes aegypti: avoidance of a previously attractive odor or surface color that is paired with an aversive stimulus. | associative learning has been shown in a variety of insects, including the mosquitoes culex quinquefasciatus and anopheles gambiae. this study demonstrates associative learning for the first time in aedes aegypti, an important vector of dengue, yellow fever and chikungunya viruses. this species prefers to rest on dark surfaces and is attracted to the odor of 1-octen-3-ol. after training in which a dark surface alone or a dark surface with odor was paired with electric shock, mosquitoes avoided t ... | 2012 | 22996441 |
| population genetic structure of aedes (stegomyia) aegypti (l.) at a micro-spatial scale in thailand: implications for a dengue suppression strategy. | the genetic population structure of aedes (stegomyia) aegypti (l.), the main vector of dengue virus, is being investigated in areas where a novel dengue suppression program is to be implemented. the aim of the program is to release and establish mosquito populations with impaired virus transmission capabilities. to model effects of the release and devise protocols for its implementation, information about the genetic structure of populations at a range of spatial scales is required. | 2013 | 23326609 |
| potential for extrinsic incubation temperature to alter interplay between transmission potential and mortality of dengue-infected aedes aegypti. | the extrinsic incubation period is a critical component in the assessment of arboviral transmission potential. it defines the time it takes for a mosquito to become infectious following exposure to an arbovirus. since this is a temporal process, the lifespan of a mosquito is intimately tied to the extrinsic incubation period and thus transmission potential of these viruses. temperature is a known effector of both vector competence (the ability of a vector to transmit a pathogen) and mosquito mor ... | 2016 | 27478382 |
| competitive advantage of a dengue 4 virus when co-infecting the mosquito aedes aegypti with a dengue 1 virus. | dengue viruses (denv) are comprised in four related serotypes (denv-1 to 4) and are critically important arboviral pathogens affecting human populations in the tropics. south american countries have seen the reemergence of denv since the 1970's associated with the progressive re-infestation by the mosquito vector, aedes aegypti. in french guiana, denv is now endemic with the co-circulation of different serotypes resulting in viral epidemics. between 2009 and 2010, a predominant serotype change o ... | 2016 | 27390932 |
| seasonal and geographical variation of dengue vectors in narathiwat, south thailand. | using gis-based land use map for the urban-rural division (the relative ratio of population density adjusted to relatively aedes-infested land area), we demonstrated significant independent observations of seasonal and geographical variation of aedes aegypti and aedes albopictus vectors between muang narathiwat district (urban setting) and neighbor districts (rural setting) of narathiwat, southern thailand, based on binomial distribution of aedes vectors in water-holding containers (water storag ... | 2016 | 27437001 |
| genome-based polymorphic microsatellite development and validation in the mosquito aedes aegypti and application to population genetics in haiti. | microsatellite markers have proven useful in genetic studies in many organisms, yet microsatellite-based studies of the dengue and yellow fever vector mosquito aedes aegypti have been limited by the number of assayable and polymorphic loci available, despite multiple independent efforts to identify them. here we present strategies for efficient identification and development of useful microsatellites with broad coverage across the aedes aegypti genome, development of multiplex-ready pcr groups o ... | 2009 | 20003193 |
| climate variability and increase in intensity and magnitude of dengue incidence in singapore. | dengue is currently a major public health burden in asia pacific region. this study aims to establish an association between dengue incidence, mean temperature and precipitation, and further discuss how weather predictors influence the increase in intensity and magnitude of dengue in singapore during the period 2000-2007. | 2009 | 20052380 |
| permethrin-treated clothing as protection against the dengue vector, aedes aegypti: extent and duration of protection. | dengue transmission by the mosquito vector, aedes aegypti, occurs indoors and outdoors during the day. personal protection of individuals, particularly when outside, is challenging. here we assess the efficacy and durability of different types of insecticide-treated clothing on laboratory-reared ae. aegypti. | 2015 | 26440967 |
| dengue fever as a cause of hemophagocytic lymphohistiocytosis. | dengue is endemic in more than 100 countries in southeast asia, the americas, the western pacific, africa and the eastern mediterranean regions. the virus is transmitted by aedes mosquitoes. dengue disease is the most prevalent arthropod-borne viral disease in humans and is a global and national public health concern in several countries. a seasonal pattern of dengue disease is consistently observed. the highest incidences usually correspond to the period of highest rainfall and humidity, provid ... | 2015 | 26558245 |
| contrasting genetic structure between mitochondrial and nuclear markers in the dengue fever mosquito from rio de janeiro: implications for vector control. | dengue is the most prevalent global arboviral disease that affects over 300 million people every year. brazil has the highest number of dengue cases in the world, with the most severe epidemics in the city of rio de janeiro (rio). the effective control of dengue is critically dependent on the knowledge of population genetic structuring in the primary dengue vector, the mosquito aedes aegypti. we analyzed mitochondrial and nuclear genomewide single nucleotide polymorphism markers generated via re ... | 2015 | 26495042 |
| meta-analysis of studies on chemical, physical and biological agents in the control of aedes aegypti. | aedes aegypti is a vector of international concern because it can transmit to humans three important arboviral diseases: yellow fever, dengue and chikungunya. epidemics that are repeated year after year in a variety of urban centers indicate that there are control failures, allowing the vector to continue expanding. | 2015 | 26341708 |
| assessment of the impact of potential tetracycline exposure on the phenotype of aedes aegypti ox513a: implications for field use. | aedes aegypti is the primary vector of dengue fever, a viral disease which has an estimated incidence of 390 million infections annually. conventional vector control methods have been unable to curb the transmission of the disease. we have previously reported a novel method of vector control using a tetracycline repressible self-limiting strain of ae. aegypti ox513a which has achieved >90% suppression of wild populations. | 2015 | 26270533 |
| parity and longevity of aedes aegypti according to temperatures in controlled conditions and consequences on dengue transmission risks. | in guadeloupe, aedes aegypti mosquitoes are the only vectors of dengue and chikungunya viruses. for both diseases, vector control is the only tool for preventing epidemics since no vaccine or specific treatment is available. however, to efficiently implement control of mosquitoes vectors, a reliable estimation of the transmission risks is necessary. to become infective an ae. aegypti female must ingest the virus during a blood meal and will not be able to transmit the virus during another blood- ... | 2015 | 26258684 |
| the cost of routine aedes aegypti control and of insecticide-treated curtain implementation. | insecticide-treated curtains (itcs) are promoted for controlling the dengue vector aedes aegypti. we assessed the cost of the routine aedes control program (racp) and the cost of itc implementation through the racp and health committees in venezuela and through health volunteers in thailand. the yearly cost of the racp per household amounted to us$2.14 and $1.89, respectively. the itc implementation cost over three times more, depending on the channel used. in venezuela the racp was the most eff ... | 0 | 21540384 |
| histochemical and ultrastructural studies of the mosquito aedes aegypti fat body: effects of aging and diet type. | aedes aegypti is the principal vector of dengue world wide and a major vector of urban yellow fever. despite its epidemiological importance, not much is known regarding cellular and structural changes in the fat body in this mosquito. here, we applied light and transmission electron microscopies to investigate structural changes in the fat body of three groups of a. aegypti females: newly emerged, 18-day-old sugar-fed, and 18-day-old blood-fed. the fat body consists of a layer of cells attached ... | 2011 | 21509905 |
| oviposition site selection by the dengue vector aedes aegypti and its implications for dengue control. | because no dengue vaccine or antiviral therapy is commercially available, controlling the primary mosquito vector, aedes aegypti, is currently the only means to prevent dengue outbreaks. traditional models of ae. aegypti assume that population dynamics are regulated by density-dependent larval competition for food and little affected by oviposition behavior. due to direct impacts on offspring survival and development, however, mosquito choice in oviposition site can have important consequences f ... | 2011 | 21532736 |
| evaluation of the effectiveness of insecticide treated materials for household level dengue vector control. | to assess the operational effectiveness of long-lasting insecticide treated materials (itms), when used at household level, for the control of aedes aegypti in moderately infested urban and suburban areas. | 2011 | 21468313 |
| temperature induces trade-offs between development and starvation resistance in aedes aegypti (l.) larvae. | heightened temperature increases the development rate of mosquitoes. however, in aedes aegypti (diptera: culicidae), the larvae of which commonly experience limited access to food in urban habitats, temperature effects on adult production may also be influenced by changes in the capacity of larvae to survive without food. we carried out experiments to investigate the effects of temperatures increasing at intervals of 2 °c from 20 °c to 30 °c on the growth, maturation rate and longevity of optima ... | 2011 | 21410734 |
| towards a semen proteome of the dengue vector mosquito: protein identification and potential functions. | no commercially licensed vaccine or treatment is available for dengue fever, a potentially lethal infection that impacts millions of lives annually. new tools that target mosquito control may reduce vector populations and break the cycle of dengue transmission. male mosquito seminal fluid proteins (sfps) are one such target since these proteins, in aggregate, modulate the reproduction and feeding patterns of the dengue vector, aedes aegypti. as an initial step in identifying new targets for deng ... | 2011 | 21423647 |
| genetic elimination of dengue vector mosquitoes. | an approach based on mosquitoes carrying a conditional dominant lethal gene (release of insects carrying a dominant lethal, ridl) is being developed to control the transmission of dengue viruses by vector population suppression. a transgenic strain, designated ox3604c, of the major dengue vector, aedes aegypti, was engineered to have a repressible female-specific flightless phenotype. this strain circumvents the need for radiation-induced sterilization, allows genetic sexing resulting in male-on ... | 2011 | 21383140 |
| male mating history and body size influence female fecundity and longevity of the dengue vector aedes aegypti. | male reproductive success is dependent on insemination success and reproductive output. during mating, male mosquitoes transfer not just sperm, but also seminal fluid proteins that may have profound effects on mated female biology and behavior. in this study, we investigated the role of male body size and mating history on semen depletion, female longevity, and reproductive success in aedes aegypti l. small and large males were mated in rapid succession with up to five females. our results indic ... | 0 | 21485355 |
| ecological modeling of aedes aegypti (l.) pupal production in rural kamphaeng phet, thailand. | aedes aegypti (l.) is the primary vector of dengue, the most important arboviral infection globally. until an effective vaccine is licensed and rigorously administered, ae. aegypti control remains the principal tool in preventing and curtailing dengue transmission. accurate predictions of vector populations are required to assess control methods and develop effective population reduction strategies. ae. aegypti develops primarily in artificial water holding containers. release recapture studies ... | 2011 | 21267055 |
| insecticide resistance in aedes aegypti populations from ceará, brazil. | organophosphates and pyrethroids are used widely in brazil to control aedes aegypti, the main vector of dengue viruses, under the auspices of the national programme for dengue control. resistance to these insecticides is widespread throughout brazil. in ceará the vector is present in 98% of districts and resistance to temephos has been reported previously. here we measure resistance to temephos and the pyrethroid cypermethrin in three populations from ceará and use biochemical and molecular assa ... | 2011 | 21226942 |
| the aquaporin gene family of the yellow fever mosquito, aedes aegypti. | the mosquito, aedes aegypti, is the principal vector of the dengue and yellow fever viruses. during feeding, an adult female can take up more than its own body weight in vertebrate blood. after a blood meal females excrete large amounts of urine through their excretion system, the malpighian tubules (mt). diuresis starts within seconds after the mosquito starts feeding. aquaporins (aqps) are a family of membrane transporters that regulate the flow of water, glycerol and other small molecules acr ... | 2010 | 21249121 |
| the extinction of dengue through natural vulnerability of its vectors. | dengue is the world's most important mosquito-borne viral illness. successful future management of this disease requires an understanding of the population dynamics of the vector, especially in the context of changing climates. our capacity to predict future dynamics is reflected in our ability to explain the significant historical changes in the distribution and abundance of the disease and its vector. | 2010 | 21200424 |
| a laboratory investigation into oviposition responses of aedes aegypti to some common household substances and water from conspecific larvae. | the surveillance of dengue vector - aedes aegypti, is routinely done by using ovitraps (a dark container filled with water and a wooden paddle/towel cloth provided as a oviposition substrate), which indicates the extent of aedes activity in an area. | 2011 | 27407765 |
| the dengue vector aedes aegypti contains a functional high mobility group box 1 (hmgb1) protein with a unique regulatory c-terminus. | the mosquito aedes aegypti can spread the dengue, chikungunya and yellow fever viruses. thus, the search for key molecules involved in the mosquito survival represents today a promising vector control strategy. high mobility group box (hmgb) proteins are essential nuclear factors that maintain the high-order structure of chromatin, keeping eukaryotic cells viable. outside the nucleus, secreted hmgb proteins could alert the innate immune system to foreign antigens and trigger the initiation of ho ... | 2012 | 22802955 |
| translational selection of genes coding for perfectly conserved proteins among three mosquito vectors. | the biased usage of synonymous codons affects translational efficiency of genes. we studied codon usage patterns of genes that are perfectly conserved at the amino acid level among three important mosquito vector species: aedes aegypti (vector of dengue virus), anopheles gambiae (vector of malaria) and culex quinquefasciatus (vector of lymphatic filariasis and west nile virus). although these proteins have the same amino acid sequences, non-random usage of synonymous codons is evident among the ... | 2012 | 22705463 |
| gene amplification, abc transporters and cytochrome p450s: unraveling the molecular basis of pyrethroid resistance in the dengue vector, aedes aegypti. | pyrethroid insecticides are widely utilized in dengue control. however, the major vector, aedes aegypti, is becoming increasingly resistant to these insecticides and this is impacting on the efficacy of control measures. the near complete transcriptome of two pyrethroid resistant populations from the caribbean was examined to explore the molecular basis of this resistance. | 2012 | 22720108 |
| the antioxidant role of xanthurenic acid in the aedes aegypti midgut during digestion of a blood meal. | in the midgut of the mosquito aedes aegypti, a vector of dengue and yellow fever, an intense release of heme and iron takes place during the digestion of a blood meal. here, we demonstrated via chromatography, light absorption and mass spectrometry that xanthurenic acid (xa), a product of the oxidative metabolism of tryptophan, is produced in the digestive apparatus after the ingestion of a blood meal and reaches milimolar levels after 24 h, the period of maximal digestive activity. xa formation ... | 2012 | 22701629 |
| dengue: an escalating public health problem in latin america. | dengue infection is a significant and escalating public health problem in latin america. its re-emergence and subsequent rise in the region over the past 50 years has largely been caused by a combination of a lack of political will, the radical growth of urban populations, migration flow and insufficient financial resources. its increased incidence has been compounded by climate change, poor sanitation and extreme poverty, which lead to more breeding sites of the mosquito vector aedes aegypti. i ... | 0 | 22668444 |
| the dengue situation in africa. | dengue outbreaks and epidemics have been reported in all regions of africa, and it is believed that all four dengue virus serotypes are in circulation. available data suggest that dengue is endemic to 34 african countries and that aedes aegypti mosquitoes--the primary vector for dengue transmission--are known to be present in all but five countries. whether populations in africa are susceptible to dengue at the same rates as in asia and latin america is difficult to determine from the available ... | 0 | 22668445 |
| linking oviposition site choice to offspring fitness in aedes aegypti: consequences for targeted larval control of dengue vectors. | current aedes aegypti larval control methods are often insufficient for preventing dengue epidemics. to improve control efficiency and cost-effectiveness, some advocate eliminating or treating only highly productive containers. the population-level outcome of this strategy, however, will depend on details of ae. aegypti oviposition behavior. | 2012 | 22563512 |
| community-based control of aedes aegypti by using mesocyclops in southern vietnam. | we previously reported a new community-based mosquito control strategy that resulted in elimination of aedes aegypti (linn.) in 40 of 46 communes in northern and central vietnam, and with annual recurrent total costs (direct and indirect) of only $0.28-$0.89 international dollars per person. this control strategy was extended to four provinces in southern vietnam in long an and hau giang (2004-2007) and to long an, ben tre, and vinh long (2005-2010). in a total of 14 communes with 124,743 reside ... | 0 | 22556087 |
| high-resolution crystal structure of fkbp12 from aedes aegypti. | dengue is one of the most infectious viral diseases prevalent mainly in tropical countries. the virus is transmitted by aedes species of mosquito, primarily aedes aegypti. dengue remains a challenging drug target for years as the virus eludes the immune responses. currently, no vaccines or antiviral drugs are available for dengue prevention. previous studies suggested that the immunosuppressive drug fk506 shows antimalarial activity, and its molecular target, fk506-binding protein (fkbp), was id ... | 2012 | 22517662 |
| increasing role of roof gutters as aedes aegypti (diptera: culicidae) breeding sites in guadeloupe (french west indies) and consequences on dengue transmission and vector control. | during the past ten years, the islands of guadeloupe (french west indies) are facing dengue epidemics with increasing numbers of cases and fatal occurrences. the vector aedes aegypti is submitted to intensive control, with little effect on mosquito populations. the hypothesis that important ae. aegypti breeding sites are not controlled is investigated herein. for that purpose, the roof gutters of 123 houses were systematically investigated, and the percentage of gutters positive for ae. aegypti ... | 2012 | 22548085 |
| evidence of polyandry for aedes aegypti in semifield enclosures. | female aedes aegypti are assumed to be primarily monandrous (i.e., mate only once in their lifetime), but true estimates of mating frequency have not been determined outside the laboratory. to assess polyandry in ae. aegypti with first-generation progeny from wild mosquitoes, stable isotope semen-labeled males ((15)n or (13)c) were allowed to mate with unlabeled females in semifield enclosures (22.5 m(3)) in a dengue-endemic area in southern mexico. on average, 14% of females were positive for b ... | 0 | 22492148 |
| proteome of aedes aegypti in response to infection and coinfection with microsporidian parasites. | hosts are frequently infected with more than one parasite or pathogen at any one time, but little is known as to how they respond to multiple immune challenges compared to those involving single infections. we investigated the proteome of aedes aegypti larvae following infection with either edhazardia aedis or vavraia culicis, and coinfections involving both. they are both obligate intracellular parasites belonging to the phylum microsporidia and infect natural populations of ae. aegypti. the re ... | 0 | 22837817 |
| pinpointing p450s associated with pyrethroid metabolism in the dengue vector, aedes aegypti: developing new tools to combat insecticide resistance. | pyrethroids are increasingly used to block the transmission of diseases spread by aedes aegypti such as dengue and yellow fever. however, insecticide resistance poses a serious threat, thus there is an urgent need to identify the genes and proteins associated with pyrethroid resistance in order to produce effective counter measures. in ae. aegypti, overexpression of p450s such as the cyp9j32 gene have been linked with pyrethroid resistance. our aim was to confirm the role of cyp9j32 and other p4 ... | 2012 | 22479665 |
| biogeography of the two major arbovirus mosquito vectors, aedes aegypti and aedes albopictus (diptera, culicidae), in madagascar. | in the past ten years, the indian ocean region has been the theatre of severe epidemics of chikungunya and dengue. these outbreaks coincided with a high increase in populations of aedes albopictus that outcompete its sister taxon aedes aegypti in most islands sampled. the objective of this work was to update the entomological survey of the two aedes species in the island of madagascar which has to face these arboviroses. | 2012 | 22433186 |
| comparing statistical models to predict dengue fever notifications. | dengue fever (df) is a serious public health problem in many parts of the world, and, in the absence of a vaccine, disease surveillance and mosquito vector eradication are important in controlling the spread of the disease. df is primarily transmitted by the female aedes aegypti mosquito. we compared two statistical models that can be used in the surveillance and forecast of notifiable infectious diseases, namely, the autoregressive integrated moving average (arima) model and the knorr-held two- ... | 2012 | 22481978 |
| robust heat-inducible gene expression by two endogenous hsp70-derived promoters in transgenic aedes aegypti. | aedes aegypti is an important vector of the viruses that cause dengue fever, dengue haemorrhagic fever and yellow fever. reverse genetic approaches to the study of gene function in this mosquito have been limited by the lack of a robust inducible promoter to allow precise temporal control over a protein-encoding or hairpin rna transgene. likewise, investigations into the molecular and biochemical basis of vector competence would benefit from the ability to activate an antipathogen molecule at sp ... | 2011 | 22142225 |
| spatial stability of adult aedes aegypti populations. | vector control programs could be more efficient by identifying the location of highly productive sites of aedes aegypti. this study explored if the number of female adults of ae. aegypti in bg-sentinel traps was clustered and if their spatial distribution changed in time in two neighborhoods in san juan, puerto rico. traps were uniformly distributed across each neighborhood (130 m from each other), and samples were taken every 3 weeks. global and local spatial autocorrelations were explored. spa ... | 0 | 22144449 |
| programmed autophagy in the fat body of aedes aegypti is required to maintain egg maturation cycles. | autophagy plays a pivotal role by allowing cells to recycle cellular components under conditions of stress, starvation, development and cancer. in this work, we have demonstrated that programmed autophagy in the mosquito fat body plays a critical role in maintaining of developmental switches required for normal progression of gonadotrophic cycles. mosquitoes must feed on vertebrate blood for their egg development, with each gonadotrophic cycle being tightly coupled to a separate blood meal. as a ... | 2011 | 22125592 |
| genetics and morphology of aedes aegypti (diptera: culicidae) in septic tanks in puerto rico. | dengue viruses, primarily transmitted by the mosquito aedes aegypti (l.), affect an estimated 50-100 million people yearly. traditional approaches to control mosquito population numbers, such as the use of pesticides, have had only limited success. atypical mosquito behavior may be one reason why current vector control efforts have been less efficacious than expected. in puerto rico, for example, adult ae. aegypti have been observed emerging from septic tanks. interestingly, adults emerging from ... | 0 | 22238867 |
| spatial clustering of aedes aegypti related to breeding container characteristics in coastal ecuador: implications for dengue control. | mosquito management within households remains central to the control of dengue virus transmission. an important factor in these management decisions is the spatial clustering of aedes aegypti. we measured spatial clustering of ae. aegypti in the town of borbón, ecuador and assessed what characteristics of breeding containers influenced the clustering. we used logistic regression to assess the spatial extent of that clustering. we found strong evidence for juvenile mosquito clustering within 20 m ... | 2013 | 24002483 |
| rapid evolution of reduced receptivity to interspecific mating in the dengue vector aedes aegypti in response to satyrization by invasive aedes albopictus. | in this paper we examine the effect of reproductive interference on the dynamics of two mosquito vectors of public health concern and add to the growing literature on the strength and speed with which interspecific reproductive interference may drive evolution. recent evidence supports a role for asymmetric reproductive interference, or satyrization, in competitive displacements of aedes aegypti by aedes albopictus. however, populations of a. aegypti sympatric with a. albopictus in nature evolve ... | 0 | 24563572 |
| phylogeography of aedes aegypti (yellow fever mosquito) in south florida: mtdna evidence for human-aided dispersal. | the invasive dengue vector aedes aegypti has persisted for > 200 years in south florida in the united states. we tested the hypotheses that florida's landscape creates dispersal barriers and corridors and that long-distance human-aided dispersal structures populations of ae. aegypti. we evaluated the phylogeography of 362 individuals from florida's east and west coasts with a 760-bp (418- and 342-bp fragments of nd5 and nd4, respectively) mitochondrial sequence. populations from these two coasts ... | 2013 | 23918216 |
| a reduce and replace strategy for suppressing vector-borne diseases: insights from a deterministic model. | genetic approaches for controlling disease vectors have aimed either to reduce wild-type populations or to replace wild-type populations with insects that cannot transmit pathogens. here, we propose a reduce and replace (r&r) strategy in which released insects have both female-killing and anti-pathogen genes. we develop a mathematical model to numerically explore release strategies involving an r&r strain of the dengue vector aedes aegypti. we show that repeated r&r releases may lead to a tempor ... | 2013 | 24023839 |
| resting behaviour of aedes aegypti in trinidad: with evidence for the re-introduction of indoor residual spraying (irs) for dengue control. | historically, aedes aegypti (l.) mosquitoes were controlled/eradicated by fumigation, residual spraying and the elimination of breeding sites. however, the underlying mechanisms of how these vector populations were managed have never been evaluated. most studies report that these programs failed due to the emergence of ddt resistance in the 1950s and early 1960s. therefore, behavioural and physiological factors have never been examined to determine program success or failure. | 2013 | 24004641 |
| distribution, seasonal variation & dengue transmission prediction in sisaket, thailand. | environmental factors including weather variables may play a significant role in the transmission of dengue. this study investigated the effect of seasonal variation on the abundance of aedes aegypti and ae. albopictus larvae and explored the impact of weather variability on dengue transmission in sisaket, thailand. | 0 | 24135179 |
| investigating the genetics of bti resistance using mrna tag sequencing: application on laboratory strains and natural populations of the dengue vector aedes aegypti. | mosquito control is often the main method used to reduce mosquito-transmitted diseases. in order to investigate the genetic basis of resistance to the bio-insecticide bacillus thuringiensis subsp. israelensis (bti), we used information on polymorphism obtained from cdna tag sequences from pooled larvae of laboratory bti-resistant and susceptible aedes aegypti mosquito strains to identify and analyse 1520 single nucleotide polymorphisms (snps). of the 372 snps tested, 99.2% were validated using d ... | 2013 | 24187584 |
| detection of the v1016g mutation in the voltage-gated sodium channel gene of aedes aegypti (diptera: culicidae) by allele-specific pcr assay, and its distribution and effect on deltamethrin resistance in thailand. | resistance to pyrethroid insecticides is widespread among populations of aedes aegypti, the main vector for the dengue virus. several different point mutations within the voltage-gated sodium channel (vgsc) gene contribute to such resistance. a mutation at position 1016 in domain ii, segment 6 of the vgsc gene in ae. aegypti leads to a valine to glycine substitution (v1016g) that confers resistance to deltamethrin. | 2013 | 24059267 |
| a global airport-based risk model for the spread of dengue infection via the air transport network. | the number of travel-acquired dengue infections has seen a consistent global rise over the past decade. an increased volume of international passenger air traffic originating from regions with endemic dengue has contributed to a rise in the number of dengue cases in both areas of endemicity and elsewhere. this paper reports results from a network-based risk assessment model which uses international passenger travel volumes, travel routes, travel distances, regional populations, and predictive sp ... | 2013 | 24009672 |
| modeling the non-stationary climate dependent temporal dynamics of aedes aegypti. | temperature and humidity strongly affect the physiology, longevity, fecundity and dispersal behavior of aedes aegypti, vector of dengue fever. contrastingly, the statistical associations measured between time series of mosquito abundance and meteorological variables are often weak and contradictory. here, we investigated the significance of these relationships at different time scales. | 2013 | 23976939 |
| multi-function oxidases are responsible for the synergistic interactions occurring between repellents and insecticides in mosquitoes. | with the spread of pyrethroid resistance in mosquitoes, the combination of an insecticide (carbamate or organophosphate) with a repellent (deet) is considered as a promising alternative strategy for the treatment of mosquito nets and other relevant materials. the efficacy of these mixtures comes from the fact that they reproduce pyrethroid features and that positive interactions occur between insecticides and repellent. to better understand the mechanisms involved and assess the impact of detoxi ... | 2009 | 19371420 |
| awareness and support of release of genetically modified "sterile" mosquitoes, key west, florida, usa. | after a dengue outbreak in key west, florida, during 2009-2010, authorities, considered conducting the first us release of male aedes aegypti mosquitoes genetically modified to prevent reproduction. despite outreach and media attention, only half of the community was aware of the proposal; half of those were supportive. novel public health strategies require community engagement. | 0 | 25625795 |
| use of insecticide-treated house screens to reduce infestations of dengue virus vectors, mexico. | dengue prevention efforts rely on control of virus vectors. we investigated use of insecticide-treated screens permanently affixed to windows and doors in mexico and found that the screens significantly reduced infestations of aedes aegypti mosquitoes in treated houses. our findings demonstrate the value of this method for dengue virus vector control. | 0 | 25625483 |
| standard operating procedures for standardized mass rearing of the dengue and chikungunya vectors aedes aegypti and aedes albopictus (diptera: culicidae) - i - egg quantification. | quantification of eggs prior to rearing the immature stages of mosquitoes is an essential step in establishing a standardized mass rearing system. to develop a simple and accurate method of egg quantification for aedes aegypti and aedes albopictus, the relationship between egg number and weight, as well as egg number and volume, were studied. | 2015 | 25614052 |