| the transcription factor foxo1 controls central-memory cd8+ t cell responses to infection. | memory t cells protect hosts from pathogen reinfection, but how these cells emerge from a pool of antigen-experienced t cells is unclear. here, we show that mice lacking the transcription factor foxo1 in activated cd8+ t cells have defective secondary, but not primary, responses to listeria monocytogenes infection. compared to short-lived effector t cells, memory-precursor t cells expressed higher amounts of foxo1, which promoted their generation and maintenance. chromatin immunoprecipitation se ... | 2013 | 23932570 |
| role of the listeria monocytogenes 2-cys peroxiredoxin homologue in protection against oxidative and nitrosative stress and in virulence. | peroxiredoxins contribute to protection of some bacteria against reactive oxygen intermediates (rois) and reactive nitrogen intermediates (rnis). listeria monocytogenes, a facultative intracellular bacterial pathogen, interacts with rois and rnis during infection. in this study, we investigated the involvement of the 2-cys peroxiredoxin (prx) homologue in l. monocytogenes in the protection against rois and rnis and in virulence through the construction of an in-frame prx deletion mutant. the δpr ... | 2014 | 23929591 |
| subtyping of listeria monocytogenes isolates recovered from retail ready-to-eat foods, processing plants and listeriosis patients in sweden 2010. | identification and prioritisation of food safety interventions requires an understanding of the relationship between food, pathogens and cases. such understanding can be gained through different approaches, e.g. microbial subtyping to attribute cases of foodborne disease to food vehicles or other sources of illness. in this study, listeria monocytogenes isolates (n=166) from (i) three categories of ready-to-eat (rte) foods, (ii) food processing plant environments, and (iii) human listeriosis cas ... | 2013 | 23911759 |
| evaluation of antibiotic resistance patterns of food and clinical listeria monocytogenes isolates in portugal. | the aim of this study was to characterize a broad collection of isolates of listeria monocytogenes, of different serotypes, recovered in portugal between 2003 and 2007 from foods (n=353) and from clinical cases of human listeriosis (n=95), in terms of antimicrobial susceptibility. all the isolates were susceptible to ampicillin, the preferred agent to treat listeriosis. resistances to nitrofurantoin (n=99), to ciprofloxacin (n=18), to erythromycin (n=10), to tetracycline (n=2), to gentamicin (n= ... | 2013 | 23909772 |
| construction of listeria monocytogenes mutants with in-frame deletions in the phosphotransferase transport system (pts) and analysis of their growth under stress conditions. | listeria monocytogenes is a foodborne pathogen that is difficult to eliminate due to its ability to survive under different stress conditions such as low ph and high salt. to better control this pathogen in food, it is important to understand its survival mechanisms under these stress conditions. lmof2365_0442, 0443, and 0444 encode for phosphotransferase transport system (pts) permease (fructose-specific iiabc components) that is responsible for sugar transport. lmof2365_0445 encodes for glycos ... | 2013 | 23909479 |
| host range and in vitro lysis of listeria monocytogenes seafood isolates by bacteriophages. | listeria-infecting bacteriophages (listeriaphages) can be used to control listeria monocytogenes in the food industry. however, the sensitivity of many of seafood-borne listeria strains to phages has not been reported. this research investigated the host ranges of three listeriaphages (fwllm1, fwllm3 and fwllm5) by the formation of lytic zones and plaques on host lawns and in vitro lysis kinetics of listeriaphage fwllm3. the study also predicted the phage titres required to lyse host cells. the ... | 2014 | 23908393 |
| a role for sirt2-dependent histone h3k18 deacetylation in bacterial infection. | pathogens dramatically affect host cell transcription programs for their own profit during infection, but in most cases, the underlying mechanisms remain elusive. we found that during infection with the bacterium listeria monocytogenes, the host deacetylase sirtuin 2 (sirt2) translocates to the nucleus, in a manner dependent on the bacterial factor inlb. sirt2 associates with the transcription start site of a subset of genes repressed during infection and deacetylates histone h3 on lysine 18 (h3 ... | 2013 | 23908241 |
| effectiveness of phages in the decontamination of listeria monocytogenes adhered to clean stainless steel, stainless steel coated with fish protein, and as a biofilm. | listeria monocytogenes is a food-borne pathogen which causes listeriosis and is difficult to eradicate from seafood processing environments; therefore, more effective control methods need to be developed. this study investigated the effectiveness of three bacteriophages (limn4l, limn4p and limn17), individually or as a three-phage cocktail at ≈9 log₁₀ pfu/ml, in the lysis of three seafood-borne l. monocytogenes strains (19co9, 19do3 and 19eo3) adhered to a fish broth layer on stainless steel cou ... | 2013 | 23907252 |
| genetic characterization of listeria monocytogenes isolates from food processing facilities before and after postcook chiller heat treatment. | possible selection for and establishment of stress-resistant listeria monocytogenes variants as a consequence of heating interventions is of concern to the food industry. lineage analysis and multilocus variable number tandem repeat analysis (mlva) was performed on 20 l. monocytogenes isolates, of which 15 were obtained before and 5 were obtained after heat treatment of a postcook meat chiller. the ctsr gene (a class iii heat shock gene regulator) from 14 isolates was amplified and sequenced bec ... | 2013 | 23905808 |
| commercial biopreservatives combined with salt and sugar to control listeria monocytogenes during smoked salmon processing. | three commercial antimicrobials, applied during the salting stage in the preparation of cold-smoked salmon, were investigated for their effect on the behavior of listeria monocytogenes. fresh salmon inoculated with l. monocytogenes inia 2530 was treated with three bacteriocin-based commercial biopreservatives, which were applied in combination with a salt-sugar mix. the product was kept at 8°c for 7 days. l. monocytogenes grew by approximately 3 log cfu/g in control salmon (without the salt-suga ... | 2013 | 23905807 |
| inactivation of human pathogens during phase ii composting of manure-based mushroom growth substrate. | commercial production of white button mushrooms (agaricus bisporus) requires a specialized growth substrate prepared from composted agricultural by-products. because horse and poultry manures are widely used in substrate formulations, there is a need to determine the extent to which the composting process is capable of eliminating human pathogens. in this study, partially composted substrate was inoculated with a pathogen cocktail (log 10⁶ to 10⁸ cfu/g) containing listeria monocytogenes, escheri ... | 2013 | 23905795 |
| inhibitory effects of chitosan coating combined with organic acids on listeria monocytogenes in refrigerated ready-to-eat shrimps. | chitosan and organic acids are natural preservatives with proven antimicrobial efficacies. this study investigated the effects of chitosan coating combined with organic acids on inhibiting listeria monocytogenes in ready-to-eat (rte) shrimps during storage at 4°c. cooked shrimps inoculated with l. monocytogenes were coated with 0.5 and 1 % chitosan solutions in combination with 2 % organic acids (acetic, citric, lactic, and malic acids) for 5 min and then stored at 4°c for 16 days. bacterial cou ... | 2013 | 23905793 |
| identifying ingredients that delay outgrowth of listeria monocytogenes in natural, organic, and clean-label ready-to-eat meat and poultry products. | the objective of this study was to identify ingredients that inhibit listeria monocytogenes in natural, organic, or clean-label ready-to-eat meat and poultry products. fourteen ingredients were screened in uncured (no-nitrate-or-nitrite-added), traditional-cured (156 ppm of purified sodium nitrite), cultured (alternative cured, natural nitrate source, and staphylococcus carnosus), or preconverted (alternative cured, natural nitrite source) turkey slurries. slurries were cooked, cooled, inoculate ... | 2013 | 23905792 |
| fate of naturally occurring escherichia coli o157:h7 and other zoonotic pathogens during minimally managed bovine feedlot manure composting processes. | reducing escherichia coli o157:h7 in livestock manures before application to cropland is critical for reducing the risk of foodborne illness associated with produce. our objective was to determine the fate of naturally occurring e. coli o157:h7 and other pathogens during minimally managed on-farm bovine manure composting processes. feedlot pen samples were screened to identify e. coli o157:h7-positive manure. using this manure, four piles of each of three different composting formats were constr ... | 2013 | 23905785 |
| staphylococcus aureus but not listeria monocytogenes adapt to triclosan and adaptation correlates with increased fabi expression and agr deficiency. | the ability of pathogens to adapt to the widely used biocide, triclosan, varies substantially. the purpose of the study was to examine bacterial adaptation over an extended period of time to low increments of triclosan concentrations. focus was two human pathogens, s. aureus and l. monocytogenes that previously have displayed inherent high and low adaptability, respectively. | 2013 | 23898801 |
| vision loss following intraocular listeriosis associated with contaminated cantaloupe. | intraocular listeriosis, a rare manifestation of invasive listeriosis, has a poor visual prognosis. we report an intraocular listeriosis case related to a multistate outbreak associated with contaminated cantaloupe. increasing awareness of rare listeriosis presentations might facilitate timely diagnosis and treatment, and case reporting can clarify medical and epidemiologic aspects of listeriosis. | 2013 | 23898285 |
| virtual memory cd8 t cells display unique functional properties. | previous studies revealed the existence of foreign antigen-specific memory phenotype cd8 t cells in unimmunized mice. considerable evidence suggests this population, termed "virtual memory" (vm) cd8 t cells, arise via physiological homeostatic mechanisms. however, the antigen-specific function of vm cells is poorly characterized, and hence their potential contribution to immune responses against pathogens is unclear. here we show that naturally occurring, polyclonal vm cells have unique function ... | 2013 | 23898211 |
| [neurological complications during treatment of the tumor necrosis alpha inhibitors]. | medications with tnf-alpha inhibitors family are successfully applicable in rheumatology, gastroenterology, dermatology and neurology. still, the ongoing research on the safety assessment of their application, also due to neurological complications. the vast majority of these complications is associated with an increased risk of serious virus (herpes simplex--jc) and bacterial (listeria monocytogenes) neuroinfections. they can cause the occurrence of progressive multifocal leukoencephalopathy--p ... | 2013 | 23894783 |
| role of a gntr-family response regulator lbra in listeria monocytogenes biofilm formation. | the formation of listeria monocytogenes biofilms contributes to persistent contamination in food processing facilities. a microarray comparison of l. monocytogenes between the transcriptome of the strong biofilm forming strain (bfm(s)) scott a and the weak biofilm forming (bfm(w)) strain f2365 was conducted to identify genes potentially involved in biofilm formation. among 951 genes with significant difference in expression between the two strains, a gntr-family response regulator encoding gene ... | 2013 | 23894658 |
| conservation and distribution of the benzalkonium chloride resistance cassette bcrabc in listeria monocytogenes. | analysis of a panel of 116 listeria monocytogenes strains of diverse serotypes and sources (clinical, environment of food processing plants, and food) revealed that all but one of the 71 benzalkonium chloride-resistant (bc(r)) isolates harbored bcrabc, previously identified on a large plasmid (plm80) of the 1998-1999 hot dog outbreak strain h7858. in contrast, bcrabc was not detected among bc-susceptible (bc(s)) isolates. the bcrabc sequences were highly conserved among strains of different sero ... | 2013 | 23892748 |
| comparison of listeria monocytogenes exoproteomes from biofilm and planktonic state: lmo2504, a protein associated with biofilms. | the food-borne pathogen listeria monocytogenes is the causative agent of the severe human and animal disease listeriosis. the persistence of this bacterium in food processing environments is mainly attributed to its ability to form biofilms. the search for proteins associated with biofilm formation is an issue of great interest, with most studies targeting the whole bacterial proteome. nevertheless, exoproteins constitute an important class of molecules participating in various physiological pro ... | 2013 | 23892746 |
| fluorescent labeling of nisin z and assessment of anti-listerial action. | biomolecule labeling by fluorescent markers has emerged as an innovative methodology for bio-analytical purposes in food microbiology, medicine and pharmaceutics due to the great advantages of this method such as precision, wide detection limits, and in vivo recognition. fluorescent nisin z was synthesized by linking the carboxyl group and amino group of nisin z and 5-aminoacetamido fluorescein (aaa-flu). this new structure was fully characterized by mass spectrometry with a molecular weight of ... | 2013 | 23892059 |
| γδ t cells exhibit multifunctional and protective memory in intestinal tissues. | the study of t cell memory and the target of vaccine design have focused on memory subsumed by t cells bearing the αβ t cell receptor. alternatively, γδ t cells are thought to provide rapid immunity, particularly at mucosal borders. here, we have shown that a distinct subset of mucosal γδ t cells mounts an immune response to oral listeria monocytogenes (lm) infection and leads to the development of multifunctional memory t cells capable of simultaneously producing interferon-γ and interleukin-17 ... | 2013 | 23890071 |
| effect of different concentrations of acetic, citric, and propionic acid dipping solutions on bacterial contamination of raw chicken skin. | bacterial contamination of raw, processed poultry may include spoilage bacteria and foodborne pathogens. we evaluated different combinations of organic acid (oa) wash solutions for their ability to reduce bacterial contamination of raw chicken skin and to inhibit growth of spoilage bacteria and pathogens on skin during refrigerated storage. in experiment 1, raw chicken skin samples were dipped into a suspension of either 10(8) cfu/ml of salmonella typhimurium, escherichia coli o157:h7, or lister ... | 2013 | 23873572 |
| combining individual-based modeling and food microenvironment descriptions to predict the growth of listeria monocytogenes on smear soft cheese. | an individual-based modeling (ibm) approach was developed to describe the behavior of a few listeria monocytogenes cells contaminating smear soft cheese surface. the ibm approach consisted of assessing the stochastic individual behaviors of cells on cheese surfaces and knowing the characteristics of their surrounding microenvironments. we used a microelectrode for ph measurements and micro-osmolality to assess the water activity of cheese microsamples. these measurements revealed a high variabil ... | 2013 | 23872572 |
| dna extraction protocol for rapid pcr detection of pathogenic bacteria. | twelve reagents were evaluated to develop a direct dna extraction method suitable for pcr detection of foodborne bacterial pathogens. many reagents exhibited strong pcr inhibition, requiring significant dilution of the extract with a corresponding reduction in sensitivity. most reagents also exhibited much lower recovery of dna from the gram-positive test organism (listeria monocytogenes) than from the gram-negative organism (escherichia coli o157:h7), preventing unbiased detection and quantitat ... | 2013 | 23872000 |
| neonatal listeriosis followed by nosocomial infection. | neonatal listeriosis is widely reported, but this is the first case reported in serbia. a newborn developed respiratory distress syndrome 2 hours after delivery and was admitted to the neonatal intensive care unit. initial empirical therapy was inappropriate. consequently, on the second day, the patient developed meningitis. listeria monocytogenes was isolated from the tracheal aspirate, blood, periumbilical swab, and cerebrospinal fluid. after bacteriology results, the therapy was changed to am ... | 2014 | 23867680 |
| molecular mechanisms of cell-cell spread of intracellular bacterial pathogens. | several bacterial pathogens, including listeria monocytogenes, shigella flexneri and rickettsia spp., have evolved mechanisms to actively spread within human tissues. spreading is initiated by the pathogen-induced recruitment of host filamentous (f)-actin. f-actin forms a tail behind the microbe, propelling it through the cytoplasm. the motile pathogen then encounters the host plasma membrane, forming a bacterium-containing protrusion that is engulfed by an adjacent cell. over the past two decad ... | 2013 | 23864553 |
| targeting listeria monocytogenes rpoa and rpod genes using peptide nucleic acids. | treating intracellular pathogens remains a considerable medical challenge because of the inefficient intracellular delivery of antimicrobials and the frequent emergence of bacterial resistance to therapeutic agents deemed the drugs of last resort. we investigated the capability of antisense peptide nucleic acids (pnas) conjugated to the (kff)3k cell penetrating peptide to target rna polymerase α subunit (rpoa) and rna polymerase sigma 70 (rpod) in the intracellular pathogen listeria monocytogene ... | 2013 | 23859300 |
| [comparative proteomics analysis of extracellular proteins from listeria monocytogenes and its isogenic prfa deletion mutant]. | positive regulatory factor a (prfa) protein plays a key role in the pathogenicity of listeria monocytogenes by regulating the expression of virulence genes. we studied the regulation functions of prfa and its role in listeria monocytogenes (lm) virulence. | 2013 | 23858714 |
| the bioluminescent listeria monocytogenes strain xen32 is defective in flagella expression and highly attenuated in orally infected balb/cj mice. | in vivo bioluminescence imaging (bli) is a powerful method for the analysis of host-pathogen interactions in small animal models. the commercially available bioluminescent listeria monocytogenes strain xen32 is commonly used to analyse immune functions in knockout mice and pathomechanisms of listeriosis. | 2013 | 23856386 |
| nisin resistance of listeria monocytogenes is increased by exposure to salt stress and is mediated via liar. | growth of listeria monocytogenes on refrigerated, ready-to-eat food is a significant food safety concern. natural antimicrobials, such as nisin, can be used to control this pathogen on food, but little is known about how other food-related stresses may impact how the pathogen responds to these compounds. prior work demonstrated that exposure of l. monocytogenes to salt stress at 7°c led to increased expression of genes involved in nisin resistance, including the response regulator liar. we hypot ... | 2013 | 23851083 |
| detection of listeria spp. in liquid egg products and in the egg breaking plants environment and tracking of listeria monocytogenes by pfge. | human listeriosis, caused by listeria monocytogenes, is a severe bacterial infection that can lead to meningitis, cerebromeningitis, bacteremia or septicemia, with acute lethality and potentially leading to death. a study has shown that 29.5% of the caged laying hens in france are contaminated by l. monocytogenes (chemaly et al., 2008). however, very little information regarding egg and egg product contamination is currently available. the objective of this study is to determine the sanitary sta ... | 2013 | 23850854 |
| listeria septicemia accompanied by central nervous system involvement in a patient with multiple myeloma and secondary diabetes. | female, 58 final diagnosis: listeria septicemia symptoms: nausea • vomitting • high fever • apathetic intelligence • repeated convulsion | 2013 | 23847709 |
| antimicrobial properties of lauric arginate alone or in combination with essential oils in tryptic soy broth and 2% reduced fat milk. | the objective of this study was to evaluate the antimicrobial activity of lauric arginate (lae) when used alone or in combination with the essential oil (eo) from cinnamon leaf and eo components, thymol and eugenol. minimum inhibitory concentrations (mics) and minimum bactericidal concentrations (mbcs) for listeria monocytogenes, escherichia coli o157:h7 and salmonella enteritidis were determined by the microbroth dilution method in tryptic soy broth (tsb) at their optimal growth temperatures. t ... | 2013 | 23845430 |
| effects of processing and storage on pediococcus pentosaceus sb83 in vaginal formulations: lyophilized powder and tablets. | vaginal probiotics have an important role in preventing the colonization of the vagina by pathogens. this study aimed to investigate different formulations with pediococcus pentosaceus sb83 (lyophilized powder and tablets with and without retarding polymer) in order to verify its stability and antilisterial activity after manufacture and during storage. the bacteriocinogenic activity of p. pentosaceus sb83 against listeria monocytogenes was evaluated in simulated vaginal fluid. suspension of ped ... | 2013 | 23844367 |
| isolation and characterization of listeria monocytogenes isolates from retail foods in shaanxi province, china. | listeria monocytogenes is an important foodborne pathogen of public health concern. a total of 902 retail food samples, including 342 ready-to-eat (rte) foods, 366 infant foods, and 194 raw chickens were collected randomly in supermarkets and farmers' markets in 12 geographic areas in shaanxi province, china and screened for l. monocytogenes. all l. monocytogenes isolates were characterized by antimicrobial susceptibility testing, serotyping, and pulsed-field gel electrophoresis (pfge). twenty-s ... | 2013 | 23841657 |
| protein level identification of the listeria monocytogenes sigma h, sigma l, and sigma c regulons. | transcriptional regulation by alternative sigma (σ) factors represents an important mechanism that allows bacteria to rapidly regulate transcript and protein levels in response to changing environmental conditions. while the role of the alternative σ factor σb has been comparatively well characterized in l. monocytogenes, our understanding of the roles of the three other l. monocytogenes alternative σ factors is still limited. in this study, we employed a quantitative proteomics approach using i ... | 2013 | 23841528 |
| visualization of cytolytic t cell differentiation and granule exocytosis with t cells from mice expressing active fluorescent granzyme b. | to evaluate acquisition and activation of cytolytic functions during immune responses we generated knock in (ki) mice expressing granzyme b (gzmb) as a fusion protein with red fluorescent tdtomato (gzmb-tom). as for gzmb in wild type (wt) lymphocytes, gzmb-tom was absent from naïve cd8 and cd4 t cells in gzmb-tom-ki mice. it was rapidly induced in most cd8 t cells and in a subpopulation of cd4 t cells in response to stimulation with antibodies to cd3/cd28. a fraction of splenic nk cells expresse ... | 2013 | 23840635 |
| route of infection determines the impact of type i interferons on innate immunity to listeria monocytogenes. | listeria monocytogenes is a food-borne pathogen which causes mild to life threatening disease in humans. ingestion of contaminated food delivers the pathogen to the gastrointestinal tract, where it crosses the epithelial barrier and spreads to internal organs. type i interferons (ifn-i) are produced during infection and decrease host resistance after systemic delivery of l. monocytogenes. here we show that mice benefit from ifn-i production following infection with l. monocytogenes via the gastr ... | 2013 | 23840314 |
| antibacterial activity of head-to-head bis-benzimidazoles. | symmetric bis-benzimidazole (bbz) conjugates were profiled for activity against a range of gram-positive and gram-negative bacteria. para-substituted ethoxy, amino and methoxy derivatives displayed potent bacteriostatic activity against meticillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, streptococci and listeria monocytogenes. moderate to good activity was also found against mycobacteria; two compounds were strongly active against logarithmic phase and hypoxia-induced ... | 2013 | 23837928 |
| prfa-like transcription factor gene lmo0753 contributes to l-rhamnose utilization in listeria monocytogenes strains associated with human food-borne infections. | listeria monocytogenes is a food-borne bacterial pathogen and the causative agent of human and animal listeriosis. among the three major genetic lineages of l. monocytogenes (i.e., li, lii, and liii), li and lii are predominantly associated with food-borne listeriosis outbreaks, whereas liii is rarely implicated in human infections. in a previous study, we identified a crp/fnr family transcription factor gene, lmo0753, that was highly specific to outbreak-associated li and lii but absent from li ... | 2013 | 23835178 |
| relationship between listeria monocytogenes and listeria spp. in seafood processing plants. | the objective of this study was to evaluate the relationship between prevalence of listeria monocytogenes as an outcome and listeria spp. as an explanatory variable by food products, food contact surfaces, and nonfood contact surfaces in seafood processing plants by using peer-reviewed published data. nine sets of prevalence data of l. monocytogenes and listeria spp. were collected from published studies and used for the analyses. based on our analysis, the relationship between l. monocytogenes ... | 2013 | 23834807 |
| effect of age of cook-in-bag delicatessen meats formulated with lactate-diacetate on the behavior of listeria monocytogenes contamination introduced when opening the packages during storage. | this study evaluated the potential effect of age of cook-in-bag ham and turkey breast delicatessen meats formulated with lactate-diacetate on survival and/or growth of listeria monocytogenes introduced after opening of packages and slicing of product. commercially prepared cured ham and turkey breast products formulated with potassium lactate and sodium diacetate were stored at 1.7°c unsliced, in their original cook-in-bags, and without postlethality exposure. on days 5, 90, 120, and 180 of stor ... | 2013 | 23834806 |
| determination of 5-log reduction times for escherichia coli o157:h7, salmonella enterica, or listeria monocytogenes in acidified foods with ph 3.5 or 3.8 3. | a critical factor in ensuring the safety of acidified foods is the establishment of a thermal process that assures the destruction of acid-resistant vegetative pathogenic and spoilage bacteria. for acidified foods such as dressings and mayonnaises with ph values of 3.5 or higher, the high water phase acidity (acetic acid of 1.5 to 2.5% or higher) can contribute to lethality, but there is a lack of data showing how the use of common ingredients such as acetic acid and preservatives, alone or in c ... | 2013 | 23834800 |
| biofilm formation and resistance to benzalkonium chloride in listeria monocytogenes isolated from a fish processing plant. | listeria monocytogenes is a foodborne pathogen that causes the potentially life-threatening illness listeriosis. previously, a few clones of l. monocytogenes persisting in a cold-smoked fish processing plant were isolated from the plant's products continuously. to evaluate the role of biofilms in the persistence of l. monocytogenes strains specific to this plant, the abilities of the persistent strain (ps) and transient strain (ts) of l. monocytogenes found in this plant to form biofilms were co ... | 2013 | 23834792 |
| characterization and antimicrobial properties of water chestnut starch-chitosan edible films. | the characterization and antimicrobial properties of water chestnut starch-chitosan (wsc) films containing cornus officinalis fruit extract (coe 1% w/w), glycerol monolaurate (gml 1% w/w), nisin (10,000 iu/g), pine needle essential oil (pneo 0.35% v/v), and their combinations were evaluated. incorporation of coe decreased ph value of the film-forming solution, the moisture content and the water absorption expansion ability (waea). gml-incorporated film had lower waea, tensile strength, elongatio ... | 2013 | 23831899 |
| the effect of milk components and storage conditions on the virulence of listeria monocytogenes as determined by a caco-2 cell assay. | nearly all cases of human listeriosis have been associated with consumption of contaminated food, therefore the investigation of the virulence of listeria (l.) monocytogenes after exposure to environmental conditions in food matrices is critical in order to understand and control its impact on public health. as milk and dairy products have been implicated in more than half of the listeriosis outbreaks, we investigated the in vitro virulence of l. monocytogenes incubated in different milk types a ... | 2013 | 23831732 |
| a rapid method for the detection of foodborne pathogens by extraction of a trace amount of dna from raw milk based on amino-modified silica-coated magnetic nanoparticles and polymerase chain reaction. | a method based on amino-modified silica-coated magnetic nanoparticles (asmnps) and polymerase chain reaction (pcr) was developed to rapidly and sensitively detect foodborne pathogens in raw milk. after optimizing parameters such as ph, temperature, and time, a trace amount of genomic dna of pathogens could be extracted directly from complex matrices such as raw milk using asmnps. the magnetically separated complexes of genomic dna and asmnps were directly subjected to single pcr (s-pcr) or multi ... | 2013 | 23830426 |
| inadequate management of pregnancy-associated listeriosis: lessons from four case reports. | listeria monocytogenes infection during pregnancy can lead to dramatic fetal or neonatal outcomes. no clinical trial has evaluated treatment options, and retrospective studies of cases are therefore important to define optimal regimens. we report four cases of materno-neonatal listeriosis illustrating inadequate antimicrobial therapy management and discuss recommended treatment options. | 2014 | 23829227 |
| cyld enhances severe listeriosis by impairing il-6/stat3-dependent fibrin production. | the facultative intracellular bacterium listeria monocytogenes (lm) may cause severe infection in humans and livestock. control of acute listeriosis is primarily dependent on innate immune responses, which are strongly regulated by nf-κb, and tissue protective factors including fibrin. however, molecular pathways connecting nf-κb and fibrin production are poorly described. here, we investigated whether the deubiquitinating enzyme cyld, which is an inhibitor of nf-κb-dependent immune responses, r ... | 2013 | 23825949 |
| systemic infection of mice with listeria monocytogenes to characterize host immune responses. | listeria monocytogenes is a gram-positive facultative intracellular bacterium that is widely used to characterize bacterial pathogenesis and host immunity. here, we describe a set of basic methods and techniques to infect mice with l. monocytogenes, measure bacterial load in tissues, and analyze immune cell subsets responding to infection in the spleen and liver. in addition, a specialized method for immune cell depletion is incorporated within the overall protocol, along with suggestions at var ... | 2013 | 23824896 |
| ifn-γ production by cd27⁺ nk cells exacerbates listeria monocytogenes infection in mice by inhibiting granulocyte mobilization. | natural killer (nk) cells are key components of the immune system involved in several immune reactions, including the clearance of intracellular pathogens. when activated, nk cells rapidly secrete particular cytokines that activate innate immunity and facilitate development of adaptive responses. conflicting reports on the role of nk cells during infection by listeria monocytogenes can be found in the literature. here, we demonstrate that during lethal infection by l. monocytogenes, activation o ... | 2013 | 23818011 |
| covalent immobilization of lysozyme on ethylene vinyl alcohol films for nonmigrating antimicrobial packaging applications. | the objective of this study was to develop a new antimicrobial film, in which lysozyme was covalently attached onto two different ethylene vinyl alcohol copolymers (evoh 29 and evoh 44). the evoh surface was modified with uv irradiation treatment to generate carboxylic acid groups, and lysozyme was covalently attached to the functionalized polymer surface. surface characterization of control and modified films was performed using attenuated total reflectance fourier transform infrared spectrosco ... | 2013 | 23815412 |
| listeria monocytogenes responds to cell density as it transitions to the long-term-survival phase. | listeria monocytogenes was recently found to enter a long-term-survival (lts) phase, which may help explain its persistence in natural environments and within food processing plants. the purpose of this study was to investigate the effects of initial cell density, initial ph and type of broth (fresh vs. spent) on the transition of l. monocytogenes to the lts phase and model the change in viable population density with time. initial cell density (~10(6)-~10(10)cfu/ml) and initial ph (5.36-6.85) b ... | 2013 | 23810956 |
| efficacy of different antimicrobials on inhibition of listeria monocytogenes growth in laboratory medium and on cold-smoked salmon. | listeria monocytogenes is of particular concern in cold-smoked fish products as it can survive curing and cold-smoking, and can subsequently grow from low numbers to potentially hazardous levels during refrigerated storage. the purpose of this study was to (i) quantify the effects of organic acids, nisin, and their combinations on controlling l. monocytogenes growth on cold-smoked salmon at refrigeration temperatures, (ii) identify synergistic interactions of binary combinations of these antimic ... | 2013 | 23803569 |
| diversity assessment of listeria monocytogenes biofilm formation: impact of growth condition, serotype and strain origin. | the foodborne pathogen listeria monocytogenes has the ability to produce biofilms in food-processing environments and then contaminate food products, which is a major concern for food safety. the biofilm forming behavior of 143 l. monocytogenes strains was determined in four different media that were rich, moderate or poor in nutrients at 12°c, 20°c, 30°c and 37°c. the biofilm formation was mostly influenced by temperature, resulting in decreased biofilm formation with decreasing temperature. bi ... | 2013 | 23800738 |
| secreted listeria adhesion protein (lap) influences lap-mediated listeria monocytogenes paracellular translocation through epithelial barrier. | listeria adhesion protein (lap), an alcohol acetaldehyde dehydrogenase (lmo1634) promotes bacterial paracellular translocation through epithelial cell junctions during gastrointestinal phase of infection. secreted lap is critical for pathogenesis and is mediated by seca2 system; however, if strain dependent variation in lap secretion would affect l. monocytogenes paracellular translocation through epithelial barrier is unknown. | 2013 | 23799938 |
| a bifunctional invertebrate-type lysozyme from the disk abalone, haliotis discus discus: genome organization, transcriptional profiling and biological activities of recombinant protein. | lysozyme is an important enzyme in the innate immune system that plays a vital role in fighting microbial infections. in the current study, we identified, cloned, and characterized a gene that encodes an invertebrate-type lysozyme from the disk abalone, haliotis discus discus (ablysi). the full-length cdna of ablysi consisted of 545 bp with an open reading frame of 393 bp that encodes 131 amino acids. the theoretical molecular mass of mature ablysi was 12.3 kda with an isoelectric point of 8.03. ... | 2013 | 23796790 |
| pathogen-specific treg cells expand early during mycobacterium tuberculosis infection but are later eliminated in response to interleukin-12. | thymically derived foxp3⁺ regulatory t (treg) cells have a propensity to recognize self-peptide:mhc complexes, but their ability to respond to epitope-defined foreign antigens during infectious challenge has not been demonstrated. here we show that pulmonary infection with mycobacterium tuberculosis (mtb), but not listeria monocytogenes (lm), induced robust lymph node expansion of a highly activated population of pathogen-specific treg cells from the pre-existing pool of thymically derived treg ... | 2013 | 23791647 |
| a case of foodborne listeriosis linked to a contaminated food-production process. | we report a case of listeriosis linked to consumption of contaminated ox tongue. a public health investigation identified intermittent contamination at a meat-production process and ox-tongue production was discontinued. sensitive molecular subtyping methods are improving our ability to track sources of listeria monocytogenes contamination through the food chain. detailed investigation of sporadic cases of listeriosis can provide important public health information and its wider use is encourage ... | 2013 | 23788596 |
| species-specific pamp recognition by tlr2 and evidence for species-restricted interaction with dectin-1. | tlrs mediate recognition of a wide range of microbial products, including lps, lipoproteins, flagellin, and bacterial dna, and signaling through tlrs leads to the production of inflammatory mediators. in addition to tlrs, many other surface receptors have been proposed to participate in innate immunity and microbial recognition, and signaling through some of these, for example, c-type lectins, is likely to cooperate with tlr signaling in defining inflammatory responses. in the present study, we ... | 2013 | 23787127 |
| growth and membrane fluidity of food-borne pathogen listeria monocytogenes in the presence of weak acid preservatives and hydrochloric acid. | this study addresses a major issue in microbial food safety, the elucidation of correlations between acid stress and changes in membrane fluidity of the pathogen listeria monocytogenes. in order to assess the possible role that membrane fluidity changes play in l. monocytogenes tolerance to antimicrobial acids (acetic, lactic, hydrochloric acid at low ph or benzoic acid at neutral ph), the growth of the bacterium and the gel-to-liquid crystalline transition temperature point (t m) of cellular li ... | 2013 | 23785360 |
| in vivo targeting of human dc-sign drastically enhances cd8⁺ t-cell-mediated protective immunity. | vaccination is one of the oldest yet still most effective methods to prevent infectious diseases. however, eradication of intracellular pathogens and treatment of certain diseases like cancer requiring efficient cytotoxic immune responses remain a medical challenge. in mice, a successful approach to induce strong cytotoxic cd8⁺ t-cell (ctl) reactions is to target antigens to dcs using specific antibodies against surface receptors in combination with adjuvants. a major drawback for translating th ... | 2013 | 23784881 |
| risk-based approach for microbiological food safety management in the dairy industry: the case of listeria monocytogenes in soft cheese made from pasteurized milk. | according to codex alimentarius commission recommendations, management options applied at the process production level should be based on good hygiene practices, haccp system, and new risk management metrics such as the food safety objective. to follow this last recommendation, the use of quantitative microbiological risk assessment is an appealing approach to link new risk-based metrics to management options that may be applied by food operators. through a specific case study, listeria monocyto ... | 2014 | 23777564 |
| efficacy of malic acid against listeria monocytogenes attached to poultry skin during refrigerated storage. | this work evaluated the effect of malic acid washing on the growth of listeria monocytogenes on poultry legs stored at 4°c for 8 d. fresh inoculated chicken legs were dipped into a 1 or 2% malic acid solution (vol/vol) for 5 min or distilled water (control). surface ph values, sensorial characteristics (odor, color, texture, and overall appearance) and l. monocytogenes, mesophile, psychrotroph, and enterobacteriaceae counts were evaluated after treatment (d 0) and after 1, 3, 6, and 8 d of stora ... | 2013 | 23776283 |
| effect of combining nisin with modified atmosphere packaging on inhibition of listeria monocytogenes in ready-to-eat turkey bologna. | abstract the objective of this study was to evaluate the effect of nisin in combination with different types of packaging on the survival of listeria monocytogenes in ready-to-eat low-fat turkey bologna. bologna was inoculated with l. monocytogenes exposed to 1 of 6 treatments: 3 packaging treatments (100% co2, air, vacuum), each with and without nisin. bologna was refrigerated and sampled 9 times over 42 d. nisin reduced initial l. monocytogenes populations by 1.5 to 2 log cycles and 100% co2 p ... | 2013 | 23776282 |
| anthropogenic impact on the presence of l. monocytogenes in soil, fruits, and vegetables. | the aim of this study was to determine the prevalence of listeria sp. and listeria monocytogenes in soil samples with reference to type of fertilizers (natural and artificial) and distance from places intensively exploited by men, as well as to determine the relationship between the presence of l. monocytogenes in the soil and in fruits and vegetables. the examined 1,000 soil samples originated from 15 different areas, whilst 140 samples of fruits and 210 samples of vegetables were collected fro ... | 2014 | 23775320 |
| an ecological perspective of listeria monocytogenes biofilms in food processing facilities. | listeria monocytogenes can enter the food chain at virtually any point. however, food processing environments seem to be of particular importance. from an ecological point of view, food processing facilities are microbial habitats that are constantly disturbed by cleaning and sanitizing procedures. although l. monocytogenes is considered ubiquitous in nature, it is important to recognize that not all l. monocytogenes strains appear to be equally distributed; the distribution of the organism seem ... | 2013 | 23768144 |
| vidas listeria monocytogenes ii (lmo2). | this aoac govval study compared the vidas listeria monocytogenes ii (lmo2) to the health products and food branch mfhpb-30 reference method for detection of l. monocytogenes in ready-to-eat (rte) meats. the vidas lmo2 test is an automated enzyme-linked fluorescent immunoassay for the detection of l. monocytogenes in foods. the lmo2 test, following the enrichment procedure from the mflp-33 method, also included use of the chromogenic media, chromid ottaviani agosti agar (oaa) and chromid lmono fo ... | 2013 | 23767346 |
| comparative evaluation of the vidas listeria monocytogenes xpress (lmx) for the detection of listeria monocytogenes in a variety of foods. | the vidas listeria monocytogenes xpress (lmx) test is an enzyme-linked fluorescent immunoassay designed for use with the automated vidas or mini-vidas instruments for the specific detection of l. monocytogenes using a 26 h proprietary enrichment broth. the vidas lmx method was validated according to harmonized aoac research institute (ri) and official methods of analysis guidelines in both the aoac performance tested method (ptm) and govval programs. in the ptm comparison studies, the vidas lmx ... | 2013 | 23767344 |
| bacterial inactivation in water, dna strand breaking, and membrane damage induced by ultraviolet-assisted titanium dioxide photocatalysis. | the effects of uv-assisted tio2-photocatalytic oxidation (pco) inactivation of pathogenic bacteria (escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium) in a liquid culture using different domains of uv irradiation (a, b and c) were evaluated. structural changes in super-coiled plasmid dna (puc19) and genomic dna of e. coli were observed using gel electrophoresis to demonstrate the photodynamic dna strand breaking activity of uv-assisted tio2-pco. membrane damage in bacteria ... | 2013 | 23764591 |
| antibiofilm effect of plant derived antimicrobials on listeria monocytogenes. | the present study investigated the efficacy of sub-inhibitory concentrations (sics, concentrations not inhibiting bacterial growth) and bactericidal concentrations (mbcs) of four, generally recognized as safe (gras), plant-derived antimicrobials (pdas) in inhibiting listeria monocytogenes (lm) biofilm formation and inactivating mature lm biofilms, at 37, 25 and 4 °c on polystyrene plates and stainless-steel coupons. in addition, the effect of sics of pdas on the expression of lm genes critical f ... | 2013 | 23764223 |
| role of initial contamination levels, biofilm maturity and presence of salt and fat on desiccation survival of listeria monocytogenes on stainless steel surfaces. | this study investigated the effect of initial contamination levels, biofilm maturity and presence of salt and fatty food soils on desiccation survival of listeria monocytogenes on stainless steel (ss) coupons. l. monocytogenes cultures grown (at 15 °c for 48 h) in tryptic soy broth with 1% glucose (tsb-glu) containing either 0.5 or 5% (w/v) nacl were re-suspended in tsb-glu containing either 0.5 or 5% nacl and used to contaminate ss coupons at levels of 3.5, 5.5, and 7.5 log cfu/cm². desiccation ... | 2013 | 23764219 |
| hurdle enhancement of slightly acidic electrolyzed water antimicrobial efficacy on chinese cabbage, lettuce, sesame leaf and spinach using ultrasonication and water wash. | slightly acidic electrolyzed water (saew) is well known as a good sanitizer against foodborne pathogens on fresh vegetables. however, microbial reductions from saew treatment are not enough to ensure produce safety. therefore, it is necessary to improve its antimicrobial efficiency by combining it with other appropriate approaches. this study examined the microbicidal activity of saew (ph 5.2-5.5, oxidation reduction potential 500-600 mv, available chlorine concentration 21-22 mg/l) on chinese c ... | 2013 | 23764218 |
| antigen experience shapes phenotype and function of memory th1 cells. | primary and secondary (boosted) memory cd8 t cells exhibit differences in gene expression, phenotype and function. the impact of repeated antigen stimulations on memory cd4 t cells is largely unknown. to address this issue, we utilized lcmv and listeria monocytogenes infection of mice to characterize primary and secondary antigen (ag)-specific th1 cd4 t cell responses. ag-specific primary memory cd4 t cells display a cd62l(lo)ccr7(hi) cd27(hi) cd127(hi) phenotype and are polyfunctional (most pro ... | 2013 | 23762323 |
| clec4f is an inducible c-type lectin in f4/80-positive cells and is involved in alpha-galactosylceramide presentation in liver. | clec4f, a member of c-type lectin, was first purified from rat liver extract with high binding affinity to fucose, galactose (gal), n-acetylgalactosamine (galnac), and un-sialylated glucosphingolipids with galnac or gal terminus. however, the biological functions of clec4f have not been elucidated. to address this question, we examined the expression and distribution of murine clec4f, determined its binding specificity by glycan array, and investigated its function using clec4f knockout (clec4f- ... | 2013 | 23762286 |
| isolation and partial characterization of bioactive fucoxanthin from himanthalia elongata brown seaweed: a tlc-based approach. | seaweeds are important sources of carotenoids, and numerous studies have shown the beneficial effects of these pigments on human health. in the present study, himanthalia elongata brown seaweed was extracted with a mixture of low polarity solvents, and the crude extract was separated using analytical thin-layer chromatography (tlc). the separated compounds were tested for their potential antioxidant capacity and antimicrobial activity against listeria monocytogenes bacteria using tlc bioautograp ... | 2013 | 23762062 |
| [incidence study of listeriosis in spain]. | we performed a descriptive retrospective study of cases of listeriosis occurring in spain from 2001 to 2007 to determine the burden and trend of this disease in our setting. several sources of information were used. epidemiological information was collected from 1.242 cases of listeriosis, representing a mean incidence rate of 0,56 cases per 100.000 inhabitants per year, which was extrapolated as an overall estimate for spain. the annual incidence showed a statistically significant increasing tr ... | 2016 | 23759185 |
| multiple supratentorial brain abscesses due to listeria monocytogenes in a patient with myasthenia gravis. | | 2013 | 23751195 |
| effector-like cd8⁺ t cells in the memory population mediate potent protective immunity. | the cd8⁺ memory t cell population is heterogeneous, and it is unclear which subset(s) optimally mediate the central goal of the immune system-protection against infection. here we investigate the protective capacities of cd8⁺ t cell subsets present at the memory stage of the immune response. we show that a population of cd8⁺ t cells bearing markers associated with effector cells (klrg1(hi), cd27(lo), t-bet(hi), eomes(lo)) persisted to the memory phase and provided optimal control of listeria mon ... | 2013 | 23746652 |
| listeria monocytogenes panophthalmitis: a novel strain follows a virulent course. | to describe the findings and clinical course of a case of listeria endophthalmitis as it progressed to panophthalmitis despite vitrectomy and intravitreal and systemic antibiotic therapy. | 2013 | 25383817 |
| combined antimicrobial effect of oregano essential oil and caprylic acid in minced beef. | oregano essential oil (oeo) and caprylic acid (ca) are highly aromatic natural antimicrobials with limited individual application in food. we proved their combined additive effect when used in meat. application of 0.5% ca and 0.2% oeo (v/w) with 0.1% of citric acid in vacuum packed minced beef inoculated with listeria monocytogenes at a concentration of 5 log cells/g reduced counts of lactic acid bacteria by 1.5 log cfu/g and counts of psychrotrophic bacteria and l. monocytogenes by more than 2. ... | 2013 | 23743028 |
| occurrence of listeria spp. in retail meat and dairy products in the area of addis ababa, ethiopia. | listeriosis, a bacterial disease in humans and animals, is mostly caused by ingestion of listeria monocytogenes via contaminated food and/or water, or by a zoonotic infection. globally, listeriosis has in general a low incidence but a high case fatality rate. | 2013 | 23742294 |
| vital signs: listeria illnesses, deaths, and outbreaks--united states, 2009-2011. | older adults, pregnant women, and persons with immunocompromising conditions are at higher risk than others for invasive listeria monocytogenes infection (listeriosis), a rare and preventable foodborne illness that can cause bacteremia, meningitis, fetal loss, and death. | 2013 | 23739339 |
| murinization of internalin extends its receptor repertoire, altering listeria monocytogenes cell tropism and host responses. | listeria monocytogenes (lm) is an invasive foodborne pathogen that leads to severe central nervous system and maternal-fetal infections. lm ability to actively cross the intestinal barrier is one of its key pathogenic properties. lm crosses the intestinal epithelium upon the interaction of its surface protein internalin (inla) with its host receptor e-cadherin (ecad). inla-ecad interaction is species-specific, does not occur in wild-type mice, but does in transgenic mice expressing human ecad an ... | 2013 | 23737746 |
| [cerebral salt wasting syndrome associated with listeria monocytogenes encephalitis. report of one case]. | hyponatremia is common in patients with severe neurological diseases and is often secondary to a syndrome of inappropriate antidiuretic hormone secretion (siadh). however, in some patients, hyponatremia is due to cerebral salt wasting syndrome (csws). siadh and csws treatments are opposite and misdiagnosis can lead to increased morbidity and mortality. we report a 52 years old female with a rhom-boencephalitis caused by listeria monocytogenes (lm), ventriculitis and abscesses in cerebellum and b ... | 2013 | 23732421 |
| single-molecule photobleaching reveals increased met receptor dimerization upon ligand binding in intact cells. | the human receptor tyrosine kinase met and its ligand hepatocyte growth factor/scatter factor are essential during embryonic development and play an important role during cancer metastasis and tissue regeneration. in addition, it was found that met is also relevant for infectious diseases and is the target of different bacteria, amongst them listeria monocytogenes that induces bacterial uptake through the surface protein internalin b. binding of ligand to the met receptor is proposed to lead to ... | 2013 | 23731667 |
| utilization of mathematical models to manage risk of holding cold food without temperature control. | this document describes the development of a tool to manage the risk of the transportation of cold food without temperature control. the tool uses predictions from combase predictor and builds on the 2009 u.s. food and drug administration model food code and supporting scientific data in the food code annex. i selected salmonella spp. and listeria monocytogenes as the organisms for risk management. salmonella spp. were selected because they are associated with a wide variety of foods and grow ra ... | 2013 | 23726207 |
| outbreak of listerosis due to imported cooked ham, switzerland 2011. | from 24 april to 31 july 2011, nine cases of listeriosis were registered in the cantons of aargau, basel-land and zurich, switzerland. in six of the cases, infection with listeria monocytogenes was laboratory confirmed, while three remained suspected cases. the suspected cases were family members of confirmed cases with identical or similar symptoms. all confirmed cases were infected with a l. monocytogenes strain belonging to serovar 1/2a: all had an indistinguishable pulsotype by pulsed-field ... | 2013 | 23725774 |
| il-18 provided in dying bacterial-infected macrophages induces ifn-γ production in functional t-cell hybridoma b6ho3 through cell conjugates. | we have previously reported that the co-culture of functional t-cell hybridoma b6ho3 with dying j774 macrophage cells infected with listeria monocytogenes (lm) results in the production of ifn-γ by b6ho3 cells. here, we explore the mechanism underlying this phenomenon. we found that ifn-γ production was dependent on il-18, but that the dying lm-infected macrophages produced no more than 100 pg/ml of il-18, much less than the amount of il-18 required for stimulating b6ho3 cells to produce ifn-γ. ... | 2014 | 23723378 |
| seca2 is not required for secretion of the surface autolysin ispc in listeria monocytogenes serotype 4b. | listeria monocytogenes is one of several gram-positive bacteria known to contain an auxiliary atpase (seca2) involved in the sec secretion of a subset of proteins important to bacterial pathogenesis, including autolysins. it is not known if ispc, a novel surface-associated autolysin essential for full virulence of l. monocytogenes serotype 4b, is seca2-dependent for secretion. by creating a seca2 gene deletion (δseca2) mutant from the wild type (wt) l. monocytogenes serotype 4b strain, in combin ... | 2014 | 23712923 |
| cytotoxic and bioactive properties of different color tulip flowers and degradation kinetic of tulip flower anthocyanins. | this study was conducted to determine the potential use of anthocyanin-based extracts (abes) of wasted tulip flowers as food/drug colorants. for this aim, wasted tulip flowers were samples and analyzed for their bioactive properties and cytotoxicity. total phenolic contents of the extracts of the claret red (126.55 mg of gallic acid equivalent (gae)/g dry extract) and orange-red (113.76 mg gae/g dry extract) flowers were the higher than those of the other tulip flowers. total anthocyanin levels ... | 2013 | 23712096 |
| mycotic aneurysms of the abdominal aorta due to listeria monocytogenes. | infected abdominal aortic aneurysms (aaas) can present insidiously with non-specific symptoms or they may present as ruptured aaa in the classical manner. | 2013 | 23711639 |
| evaluation of aerated steam treatment of alfalfa and mung bean seeds to eliminate high levels of escherichia coli o157:h7 and o178:h12, salmonella enterica, and listeria monocytogenes. | sprouts contaminated with human pathogens are able to cause food-borne diseases due to the favorable growth conditions for bacteria during germination and because of minimal processing steps prior to consumption. we have investigated the potential of hot humid air, i.e., aerated steam, to treat alfalfa and mung bean seeds which have been artificially contaminated with escherichia coli o157:h7, salmonella enterica subsp. enterica serovar weltevreden, and listeria monocytogenes scott a. in additio ... | 2013 | 23709507 |
| selective removal and inactivation of bacteria by nanoparticle composites prepared by surface modification of montmorillonite with quaternary ammonium compounds. | the purpose of the present study was to prepare new nanocomposites with antibacterial activities by surface modification of montmorillonite using quaternary ammonium compounds that are widely applied as disinfectants and antiseptics in food-processing environments. the intercalation of four quaternary ammonium compounds namely benzalkonium chloride, cetylpyridinium chloride monohydrate, hexadecyltrimethylammonium bromide, tetraethylammonium chloride hydrate into montmorillonite layers was confir ... | 2013 | 23709187 |
| galleria mellonella as a model host to study gut microbe homeostasis and brain infection by the human pathogen listeria monocytogenes. | the gastrointestinal tract in both mammals and insects is associated with microbes (collectively the microbiota), which are controlled by the intestinal immune system. these microbes regulate pathogens that can infect gut epithelial cells, and there is increasing evidence for a reciprocal relationship between beneficial and pathogenic bacteria in the gut and the intestinal immune system. deciphering these complex interactions between the microbiota and intestinal immune system in mammals require ... | 2013 | 23708825 |
| transcutaneous immunization of healthy volunteers with an attenuated listeria monocytogenes vaccine strain and cholera toxin adjuvant. | attenuated listeria monocytogenes vaccine strains have been administered intravenously (le et al., maciag et al.) and orally (angelakopoulos et al., johnson et al.) to humans. here, one was given transcutaneously with cholera toxin adjuvant. | 2013 | 23707162 |
| lactones 43. new biologically active lactones: β-cyclocitral derivatives. | in our previous studies bicyclic γ-lactones with cyclohexane ring exhibited high antifeedant activity against storage pests. the activity was correlated with the type and number of substituents in the cyclohexane ring. one of the most potent group of antifeedant agents was δ-iodo-γ-lactones. | 2014 | 23703876 |
| treatment of listeriosis in first trimester of pregnancy. | | 2013 | 23697599 |