| [cholestasis and listeriosis in the third trimester of pregnancy]. | listeriosis is an infection produced by listeria monocytogenes. it is infrequent and affects people at extreme ages, pregnant women, immunocompromised people and, occasionally, healthy people. its incidence has increased in recent years and shows a certain tendency to seasonality, increasing in summer. it can appear sporadically or as outbreaks. in pregnant women the infection is most frequently produced in the third trimester and the symptoms are usually light. nonetheless, the infection of the ... | 2014 | 24406373 |
| listeria monocytogenes persistence in food-associated environments: epidemiology, strain characteristics, and implications for public health. | over the last 10 to 15 years, increasing evidence suggests that persistence of listeria monocytogenes in food processing plants for years or even decades is an important factor in the transmission of this foodborne pathogen and the root cause of a number of human listeriosis outbreaks. l. monocytogenes persistence in other food-associated environments (e.g., farms and retail establishments) may also contribute to food contamination and transmission of the pathogen to humans. although l. monocyto ... | 2014 | 24406014 |
| biofilm formation under different temperature conditions by a single genotype of persistent listeria monocytogenes strains. | some listeria monocytogenes strains, termed persistent strains, originate from the same processing plant and have the ability to survive and grow over extended periods of time at contamination sources. in order to evaluate biofilm formation by such persistent strains, we isolated the pathogen from chicken samples collected from the same retail shop in repeated visits over 6 months. strains that were of serotype 1/2b and were assigned to the same genotype by multi-virulence-locus sequence typing ... | 2014 | 24406011 |
| prevalence of salmonella serovars, listeria monocytogenes, and escherichia coli o157:h7 in mediterranean ready-to-eat meat products in jordan. | the presence of salmonella, listeria monocytogenes, and escherichia coli o157:h7 in ready-to-eat (rte) meat products is considered a major concern for food control authorities worldwide. the aims of this study were to determine (i) the prevalence of salmonella, l. monocytogenes, and e. coli o157:h7 in mediterranean rte chicken and beef (cb) products sold in jordanian restaurants and (ii) the susceptibility of the isolates to antibiotics. a total of 1,028 samples of various types of rte cb produc ... | 2014 | 24406006 |
| listeria monocytogenes antagonizes the human gtpase cdc42 to promote bacterial spread. | the bacterial pathogen listeria monocytogenes uses actin-based motility to spread from infected human cells to surrounding healthy cells. cell-cell spread involves the formation of thin extensions of the host plasma membrane ('protrusions') containing motile bacteria. in cultured enterocytes, the listeria protein inlc promotes protrusion formation by binding and antagonizing the human scaffolding protein tuba. tuba is a known activator of the gtpase cdc42. in this work, we demonstrate an importa ... | 2014 | 24405483 |
| impedimetric detection of pathogenic gram-positive bacteria using an antimicrobial peptide from class iia bacteriocins. | real-time, label-free detection of gram-positive bacteria with high selectivity and sensitivity is demonstrated using an interdigitated impedimetric array functionalized with naturally produced antimicrobial peptide from class iia bacteriocins. the antimicrobial peptide, leucocin a, was chemically synthesized and covalently immobilized on interdigitated gold microelectrodes via the interaction between the c-terminal carboxylic acid of the peptide and free amines of a preattached thiolated linker ... | 2014 | 24400685 |
| single cell swimming dynamics of listeria monocytogenes using a nanoporous microfluidic platform. | listeria monocytogenes remains a significant foodborne pathogen due to its virulence and ability to become established in food processing facilities. the pathogen is characterized by its ability to grow over a wide temperature range and withstand a broad range of stresses. the following reports on the chemotaxis and motility of the l. monocytogenes when exposed to relatively small concentrations of acetic acid. using the developed nanoporous microfluidic device to precisely modulate the cellular ... | 2014 | 24399451 |
| highly rifampin-resistant listeria monocytogenes isolated from a patient with prosthetic bone infection. | | 2014 | 24395221 |
| sting-dependent type i ifn production inhibits cell-mediated immunity to listeria monocytogenes. | infection with listeria monocytogenes strains that enter the host cell cytosol leads to a robust cytotoxic t cell response resulting in long-lived cell-mediated immunity (cmi). upon entry into the cytosol, l. monocytogenes secretes cyclic diadenosine monophosphate (c-di-amp) which activates the innate immune sensor sting leading to the expression of ifn-β and co-regulated genes. in this study, we examined the role of sting in the development of protective cmi to l. monocytogenes. mice deficient ... | 2014 | 24391507 |
| topical cpg adjuvantation of a protein-based vaccine induces protective immunity to listeria monocytogenes. | robust cd8(+) t cell responses are essential for immune protection against intracellular pathogens. using parenteral administration of ovalbumin (ova) protein as a model antigen, the effect of the toll-like receptor 9 (tlr9) agonist, cpg oligodeoxynucleotide (odn) 1826, as an adjuvant delivered either topically, subcutaneously, or intramuscularly on antigen-specific cd8(+) t cell responses in a mouse model was evaluated. topical cpg adjuvant increased the frequency of ova-specific cd8(+) t cells ... | 2014 | 24391136 |
| control of listeria monocytogenes contamination in an iberian pork processing plant and selection of benzalkonium chloride-resistant strains. | the aims of this study were to characterize the different strains of listeria monocytogenes collected at an iberian pork processing plant and to investigate whether their specific characteristics were associated with prolonged survival in the plant. using pulsed-field gel electrophoresis (pfge), 29 pfge types were previously identified during a three-year period. eight of these pfge types persisted in the plant during that period. in the present study, a subset of 29 pfge type strains, which rep ... | 2014 | 24387856 |
| population diversity of listeria monocytogenes in quargel (acid curd cheese) lots recalled during the multinational listeriosis outbreak 2009/2010. | it has been possible to determine the genotype diversity of listeria monocytogenes in the actual cheese lots of acid curd cheese that caused a multinational outbreak between 2009 and 2010. following product recall in january 2010 all lots were investigated. a total of 422 l. monocytogenes isolates were characterized by genotyping. in a first approach the pcr serogroups were defined by multiplex-pcr assays. subsequently, the isolates were subtyped by pulsed-field gel electrophoresis (pfge) and mu ... | 2014 | 24387854 |
| inhibitory effect of combinations of caprylic acid and nisin on listeria monocytogenes in queso fresco. | queso fresco (qf), a fresh hispanic cheese has been linked to outbreaks and recalls caused by listeria contamination. the use antimicrobial treatments may be a potential solution. the goal of this research was to test the addition of nisin (n), caprylic acid (ca) and trans-cinnamaldehyde (cn) as anti-listerial ingredients in qf. qf batches were inoculated with approx. 10(4) cfu/g of 5- or 6-strain mixtures of listeria monocytogenes and treated with antimicrobials. samples were stored at 4 °c for ... | 2014 | 24387845 |
| stalling autophagy: a new function for listeria phospholipases. | listeria monocytogenes is a gram-positive bacterial pathogen that induces its own uptake in non-phagocytic cells. following invasion, listeria escapes from the entry vacuole through the secretion of a pore-forming toxin, listeriolysin o (llo) that acts to damage and disrupt the vacuole membrane. listeria then replicates in the cytosol and is able to spread from cell-to-cell using actin-based motility. in addition to llo, listeria produces two phospholipase toxins, a phosphatidylinositol-specific ... | 2014 | 28357210 |
| comparison of polymerase chain reaction methods and plating for analysis of enriched cultures of listeria monocytogenes when using the iso11290-1 method. | analysis for listeria monocytogenes by iso11290-1 is time-consuming, entailing two enrichment steps and subsequent plating on agar plates, taking five days without isolate confirmation. the aim of this study was to determine if a polymerase chain reaction (pcr) assay could be used for analysis of the first and second enrichment broths, saving four or two days, respectively. in a comprehensive approach involving six european laboratories, pcr and traditional plating of both enrichment broths from ... | 2014 | 24384162 |
| genotyping of listeria monocytogenes isolates from poultry carcasses using high resolution melting (hrm) analysis. | an outbreak situation of human listeriosis requires a fast and accurate protocol for typing listeria monocytogenes. existing techniques are either characterized by low discriminatory power or are laborious and require several days to give a final result. polymerase chain reaction (pcr) coupled with high resolution melting (hrm) analysis was investigated in this study as an alternative tool for a rapid and precise genotyping of l. monocytogenes isolates. fifty-five isolates of l. monocytogenes is ... | 2014 | 26019495 |
| irak-1 bypasses priming and directly links tlrs to rapid nlrp3 inflammasome activation. | pathogenic infections and tissue injuries trigger the assembly of inflammasomes, cytosolic protein complexes that activate caspase-1, leading to cleavage of pro-il-1β and pro-il-18 and to pyroptosis, a proinflammatory cell death program. although microbial recognition by toll-like receptors (tlrs) is known to induce the synthesis of the major caspase-1 substrate pro-il-1β, the role of tlrs has been considered limited to up-regulation of the inflammasome components. during infection with a virule ... | 2014 | 24379360 |
| nanoparticle adjuvant sensing by tlr7 enhances cd8+ t cell-mediated protection from listeria monocytogenes infection. | developing new adjuvants and vaccination strategies is of paramount importance to successfully fight against many life-threatening infectious diseases and cancer. very few adjuvants are currently authorized for human use, and these mainly stimulate a humoral response. however, specific abs are not sufficient to confer protection against persisting infections or cancer. therefore, development of adjuvants and immunomodulators able to enhance cell-mediated immune responses represents a major medic ... | 2014 | 24376264 |
| determination of shelf life for butter and cheese products in actual and accelerated conditions. | the aim of this study was to estimate the shelf life of butter and cheese products, with shelf life being a guide used to determine the storage period of food before deterioration. butter and cheese samples stored at 10℃ and 15℃ had a shelf life of 221 d, while those stored at 25℃ and 35℃ had a shelf life of 109 d. quality changes, including total cell count, coliform counts, listeria monocytogenes counts, acid value, moisture content, ph, acidity and overall sensory evaluation, were monitored. ... | 2014 | 26760945 |
| gastric fluid and heat stress response of listeria monocytogenes inoculated on frankfurters formulated with 10%, 20%, and 30% fat content. | this study evaluated the effects of frankfurter fat content on listeria monocytogenes resistance to heat stress and gastric fluid, and the caco-2 cell invasion efficiency of the pathogen. a 10-strain mixture of l. monocytogenes was inoculated on frankfurters formulated with 10%, 20%, and 30% fat content (10%: f10, 20%: f20, 30%: f30) and stored at 10℃ for 30 d. the samples were analyzed for l. monocytogenes resistance to heat stress and a simulated gastric fluid challenge. the total bacteria and ... | 2014 | 26760741 |
| preclinical efficacy and safety of an anti-il-1β vaccine for the treatment of type 2 diabetes. | neutralization of the inflammatory cytokine interleukin-1β (il-1β) is a promising new strategy to prevent the β-cell destruction, which leads to type 2 diabetes. here, we describe the preclinical development of a therapeutic vaccine against il-1β consisting of a detoxified version of il-1β chemically cross-linked to virus-like particles of the bacteriophage qβ. the vaccine was well tolerated and induced robust antibody responses in mice, which neutralized the biological activity of il-1β, as sho ... | 2014 | 26015986 |
| listeria monocytogenes - characterization of strains isolated from clinical severe cases. | listeria monocytogenes became an increasing pathogen involved more frequently in sporadic severe illnesses and outbreaks of foodborne infections. this study investigates in vitro susceptibility of 26 strains of listeria monocytogenes isolated from the clinical specimens collected between march 2009 and november 2013, from 24 patients hospitalized in three medical institutions in bucharest. all isolates were tested by disk diffusion method to 15 antimicrobial agents, by using disk diffusion tests ... | 2014 | 25870672 |
| fluxes of ca2+ and k+ are required for the listeriolysin o-dependent internalization pathway of listeria monocytogenes. | listeria monocytogenes is responsible for the life-threatening food-borne disease listeriosis. this disease mainly affects elderly and immunocompromised individuals, causing bacteremia and meningoencephalitis. in pregnant women, l. monocytogenes infection leads to abortion and severe infection of the fetus or newborn. the l. monocytogenes intracellular life cycle is critical for pathogenesis. previous studies have established that the major virulence factor of l. monocytogenes, the pore-forming ... | 2014 | 24366251 |
| listeria monocytogenes associated with new zealand seafood production and clinical cases: unique sequence types, truncated inla, and attenuated invasiveness. | listeriosis is caused by the food-borne pathogen listeria monocytogenes, which can be found in seafood and processing plants. to evaluate the risk to human health associated with seafood production in new zealand, multi-virulence-locus sequence typing (mvlst) was used to define the sequence types (sts) of 31 l. monocytogenes isolates collected from seafood-processing plants, 15 from processed foods, and 6 from human listeriosis cases. the sts of these isolates were then compared with those from ... | 2014 | 24362419 |
| combination effect of ozone and heat treatments for the inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in apple juice. | we investigated the combination effect of ozone and heat treatments in apple juice for the inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes. apple juices inoculated with the three pathogens were treated with gaseous ozone and heat simultaneously for up to 1 min. gaseous ozone treatment was progressed at a flow rate of 3.0 l/min with a concentration of 2.0-3.0 g/m³ and heat treatment was performed at temperatures of 25, 45, 50, and 55 °c. populations of ... | 2014 | 24362006 |
| improving the methodology for listeria monocytogenes detection in smoked salmon by using the wet pooling test. | controlling listeria monocytogenes in smoked salmon is still a challenge for food business operators. the reported low levels and uneven distribution of the pathogen make it necessary to apply good sampling plans and analytical procedures in order to detect contaminated batches. however, analyses are expensive and due to current economic situations, usually only one sample per lot is checked instead of the n=5 samples established in regulation (ec) no 2073/2005. this work proposes the investigat ... | 2014 | 24360294 |
| surface-conjugated antimicrobial peptide leucocin a displays high binding to pathogenic gram-positive bacteria. | leucocin a, a representative class iia bacteriocin, is a ribosomally synthesized antimicrobial peptide (amp) that displays potent activity against specific gram-positive bacteria. the antibacterial activity of such peptides is preceded by the binding event that can be utilized for studying specific peptide-bacteria interactions. in this study, 37-residue leucocin a (leua) was synthesized using solid-phase peptide synthesis and covalently immobilized on gold substrates from either the n- or c-ter ... | 2014 | 24359454 |
| listeria monocytogenes, a down-to-earth pathogen. | listeria monocytogenes is the causative agent of the food-borne life threatening disease listeriosis. this pathogenic bacterium received much attention in the endeavor of deciphering the cellular mechanisms that underlie the onset of infection and its ability to adapt to the food processing environment. although information is available on the presence of l. monocytogenes in many environmental niches including soil, water, plants, foodstuff and animals, understanding the ecology of l. monocytoge ... | 2013 | 24350062 |
| placental syncytium forms a biophysical barrier against pathogen invasion. | fetal syncytiotrophoblasts form a unique fused multinuclear surface that is bathed in maternal blood, and constitutes the main interface between fetus and mother. syncytiotrophoblasts are exposed to pathogens circulating in maternal blood, and appear to have unique resistance mechanisms against microbial invasion. these are due in part to the lack of intercellular junctions and their receptors, the achilles heel of polarized mononuclear epithelia. however, the syncytium is immune to receptor-ind ... | 2013 | 24348256 |
| reg3γ-deficient mice have altered mucus distribution and increased mucosal inflammatory responses to the microbiota and enteric pathogens in the ileum. | reg3γ is considered to have a protective role against infection with gram-positive bacteria due to its bactericidal activity, but evidence from in vivo studies is lacking. we generated a reg3γ(-/-) mouse, and investigated the effect of lack of reg3γ on intestinal mucus distribution, spatial compartmentalization of bacteria, and expression of innate immunity genes. infection studies were also performed with gram-positive and gram-negative pathogens to investigate the antimicrobial role of reg3γ. ... | 2014 | 24345802 |
| absence of growth of listeria monocytogenes in naturally contaminated cheddar cheese. | each cheese producer is responsible by the legislation for the number of listeria monocytogenes in cheese and is required to prove that numbers will not exceed 100 cfu/g throughout the shelf-life of the cheese. even in the case of hard-cheese such as cheddar cheese, the absence of growth of list. monocytogenes during ripening has to be demonstrated to comply with eu legislation. studies dedicated to assessing list. monocytogenes growth throughout cheese shelf-life are generally based on artifici ... | 2014 | 24345459 |
| aryl hydrocarbon receptor protects against bacterial infection by promoting macrophage survival and reactive oxygen species production. | aryl hydrocarbon receptor (ahr) is crucial for various immune responses. the relationship between ahr and infection with the intracellular bacteria listeria monocytogenes (lm) is poorly understood. here, we show that in response to lm infection, ahr is required for bacterial clearance by promoting macrophage survival and reactive oxygen species (ros) production. ahr-deficient mice were more susceptible to listeriosis, and ahr deficiency enhances bacterial growth in vivo and in vitro. on the othe ... | 2014 | 24343818 |
| ceftaroline activity tested against uncommonly isolated gram-positive pathogens: report from the sentry antimicrobial surveillance program (2008-2011). | ceftaroline was tested against 1859 clinically significant gram-positive organisms from uncommonly isolated species. the organisms (31 species/groups) were collected from 133 medical centres worldwide over a 4-year period (2008-2011). coagulase-negative staphylococci were generally susceptible to ceftaroline, with mic50 values (minimum inhibitory concentration required to inhibit 50% of the isolates) of 0.06-0.5mg/l. ceftaroline was active against micrococcus spp. [minimum inhibitory concentrati ... | 2014 | 24342717 |
| [listeria monocytogenes meningitis in infants: report of two cases]. | | 2013 | 24342220 |
| satureja horvatii essential oil: in vitro antimicrobial and antiradical properties and in situ control of listeria monocytogenes in pork meat. | the dominant compounds in satureja horvatii oil were p-cymene (33.14%), thymol (26.11%) and thymol methyl ether (15.08%). the minimum inhibitory concentration (mic) varied from 0.03 to 0.57 mg/ml for bacteria, and from 0.56 to 2.23 mg/ml for yeast strains, while minimum bactericidal/yeast-cidal concentration (mbc/myc) varied from 0.07 to 1.15 mg/ml and 1.11 to 5.57 mg/ml for bacteria and yeasts, respectively. the antiradical potential of the essential oil was evaluated using hydroxyl radical (•o ... | 2014 | 24342186 |
| transcription factor elf4 promotes development and function of memory cd8(+) t cells in listeria monocytogenes infection. | most differentiated cd8(+) t cells die off at the end of an infection, revealing two main subsets of memory t cells - central and effector memory - which can be found in lymphoid tissues or circulating through nonlymphoid organs, respectively. the cell intrinsic regulation of the differentiation of cd8(+) t cells to effector and central memory remains poorly studied. herein, we describe a novel role of the ets transcription factor elf4 in the development and function of memory cd8(+) t cells fol ... | 2014 | 24338897 |
| microsphere priming facilitates induction of potent therapeutic t-cell immune responses against autochthonous liver cancers. | immunotherapy of solid tumors is often hampered by the low frequency of tumor-specific t cells elicited by current vaccination strategies. here, we describe a prime-boost vaccination protocol based on the administration of antigen conjugated to poly-lactic-co-glycolic acid (plga) microspheres followed by booster vaccination with listeria monocytogenes vectors, which rapidly generates potent immune responses within two weeks. compared with conventional vaccination with antigen-pulsed dendritic ce ... | 2014 | 24338782 |
| listeria monocytogenes protein fraction induces dendritic cells maturation and t helper 1 immune responses. | fully mature dendritic cells (dcs) play pivotal role in inducing immune responses and converting naïve t lymphocytes into functional th1 cells. we aimed to evaluate listeria monocytogenes-derived protein fractions to induce dc maturation and stimulating t helper (th)1 immune responses.in the present study, we fractionated listeria monocytogenes-derived proteins by adding of ammonium sulfate in a stepwise manner. dcs were also generated from c57bl/6 mice bone marrow precursor cells. then, the eff ... | 2014 | 24338222 |
| habituation to organic acid anions induces resistance to acid and bile in listeria monocytogenes. | we evaluated the intrinsic and inducible resistance of four human pathogenic strains of listeria monocytogenes to acid and bile, factors associated with virulence. cells were grown in media at ph 7.4, or in media at ph 6.0 containing 0 (hcl control) or 4.75 mm of different organic acids, harvested at stationary or mid log phase, and challenged for 1h in acid or bile. stationary phase cells were intrinsically more resistant to either challenge than log phase cells, and large differences between s ... | 2014 | 24334034 |
| structural details of human tuba recruitment by inlc of listeria monocytogenes elucidate bacterial cell-cell spreading. | the human pathogen listeria monocytogenes is able to directly spread to neighboring cells of host tissues, a process recently linked to the virulence factor inlc. inlc targets the sixth sh3 domain (sh3-6) of human tuba, disrupting its physiological interaction with the cytoskeletal protein n-wasp. the resulting loss of cortical actin tension may slacken the junctional membrane, allowing protrusion formation by motile listeria. complexes of tuba sh3-6 with physiological partners n-wasp and mena r ... | 2014 | 24332715 |
| thymol nanoemulsified by whey protein-maltodextrin conjugates: the enhanced emulsifying capacity and antilisterial properties in milk by propylene glycol. | the objective of this research was to enhance the capability of whey protein isolate-maltodextrin conjugates in nanoemulsifying thymol using propylene glycol to improve antilisterial properties in milk. thymol was predissolved in pg and emulsified in 7% conjugate solution. transparent dispersions with mean diameters of <30 nm were observed up to 1.5%w/v thymol. in skim and 2% reduced fat milk, listeria monocytogenes scott a was reduced from ∼5 log cfu/ml to below the detection limit in 6 h by 0. ... | 2013 | 24328082 |
| the pore-forming toxin listeriolysin o is degraded by neutrophil metalloproteinase-8 and fails to mediate listeria monocytogenes intracellular survival in neutrophils. | the pore-forming toxin listeriolysin o (llo) is a major virulence factor secreted by the facultative intracellular pathogen listeria monocytogenes. this toxin facilitates l. monocytogenes intracellular survival in macrophages and diverse nonphagocytic cells by disrupting the internalization vesicle, releasing the bacterium into its replicative niche, the cytosol. neutrophils are innate immune cells that play an important role in the control of infections, yet it was unknown if llo could confer a ... | 2014 | 24319266 |
| in vitro and intracellular activities of fosfomycin against clinical strains of listeria monocytogenes. | this study was designed to evaluate the potential role of fosfomycin as a therapeutic agent in human listeriosis. the in vitro activity of fosfomycin against 154 listeria monocytogenes clinical isolates under conditions that mimic the induction of prfa expression was determined and was correlated with fosfomycin intracellular antimicrobial activity. in vitro, partial induction of prfa expression is achieved through bacterial growth in brain-heart infusion agar supplemented with activated charcoa ... | 2014 | 24315790 |
| murine cd8+ t cells but not macrophages express the vitamin d 1α-hydroxylase. | the active form of vitamin d, 1,25-dihydroxyvitamin d3 [1,25(oh)2d3] is synthesized by the 1α-hydroxylase, which is encoded by the cyp27b1 gene. using transgenic mice that have replaced the cyp27b1 gene with the bacterial lacz reporter gene (β-galactosidase), the inflammatory conditions that induce cyp27b1 in the immune system were probed. a variety of stimuli including lipopolysaccharide, anti-cd3 or pma/ionomycin were used to stimulate splenocytes and bone marrow derived macrophage in vitro. o ... | 2014 | 24314866 |
| listeria monocytogenes meningitis in an immunocompetent 18-year-old patient as a possible diagnostic and therapeutical problem. | listeria monoytogenes is the third most frequent cause of bacterial meningitis in adults. it commonly affects persons with defective cell-mediated immunity or advanced age, and only a few patiens with no underlying predisposition have been reported. | 2013 | 24313183 |
| dissociation of innate immune responses in microglia infected with listeria monocytogenes. | microglia, the innate immune cells of the brain, plays a central role in cerebral listeriosis. here, we present evidence that microglia control listeria infection differently than macrophages. infection of primary microglial cultures and murine cell lines with listeria resulted in a dual function of the two gene expression programmes involved in early and late immune responses in macrophages. whereas the bacterial gene hly seems responsible for both transcriptional programmes in macrophages, lis ... | 2014 | 24311463 |
| three-dimensional architecture of actin filaments in listeria monocytogenes comet tails. | the intracellular bacterial pathogen listeria monocytogenes is capable of remodelling the actin cytoskeleton of its host cells such that "comet tails" are assembled powering its movement within cells and enabling cell-to-cell spread. we used cryo-electron tomography to visualize the 3d structure of the comet tails in situ at the level of individual filaments. we have performed a quantitative analysis of their supramolecular architecture revealing the existence of bundles of nearly parallel hexag ... | 2013 | 24306931 |
| conditional ablation of hmgb1 in mice reveals its protective function against endotoxemia and bacterial infection. | high-mobility group box 1 (hmgb1) is a dna-binding protein abundantly expressed in the nucleus that has gained much attention for its regulation of immunity and inflammation. despite this, whether and how hmgb1 contributes to protective and/or pathological responses in vivo is unclear. in this study, we constructed hmgb1-floxed (hmgb1(f)(/f)) mice to achieve the conditional inactivation of the gene in a cell- and tissue-specific manner by crossing these mice with an appropriate cre recombinase t ... | 2013 | 24302768 |
| cerebellar abscess caused by listeria monocytogenes in a liver transplant patient. | brain abscesses are a rare but serious complication and have been documented in transplant recipients. aspergillus is by far the most frequent etiology of post-transplant brain abscesses. bacteria, apart from nocardia, have a low association with brain abscesses in transplant recipients. we report herein the case of a 52-year-old man who had undergone orthotopic liver transplantation (olt) for end-stage liver disease and hepatocellular carcinoma secondary to chronic hepatitis, and who developed ... | 2013 | 24298984 |
| impact of acid adaptation on attachment of listeria monocytogenes to stainless steel during long-term incubation under low or moderate temperature conditions and on subsequent recalcitrance of attached cells to lethal acid treatments. | this study aimed to evaluate the possible impact of acid adaptation of listeria monocytogenes cells on their attachment to stainless steel (ss) during long-term incubation under either low or moderate temperature conditions and on the subsequent recalcitrance of attached cells to lethal acid treatments. initially, nonadapted or acid-adapted stationary phase l. monocytogenes cells were used to inoculate (ca. 10⁸ cfu/ml) brain-heart infusion (bhi) broth in test tubes containing vertically placed s ... | 2014 | 24296256 |
| inhibitory activity of syzygium aromaticum and cymbopogon citratus (dc.) stapf. essential oils against listeria monocytogenes inoculated in bovine ground meat. | this research evaluated the antimicrobial effect of the clove (syzygium aromaticum) and lemongrass (cymbopogon citratus (dc.) stapf.) essential oils (eos) against listeria monocytogenes atcc 19117 growth added to bovine ground meat stored under refrigeration (5 ± 2 °c) for three days. the eos, extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (gc-ms), were tested in vitro using an agar well diffusion methodology for determination of minimum inhibitory concentrat ... | 2013 | 24294222 |
| modeling of tumor growth in dendritic cell-based immunotherapy using artificial neural networks. | exposure-response modeling and simulation is especially useful in oncology as it permits to predict and design un-experimented clinical trials as well as dose selection. dendritic cells (dc) are the most effective immune cells in the regulation of immune system. to activate immune system, dcs may be matured by many factors like bacterial cpg-dna, lipopolysaccharaide (lps) and other microbial products. in this paper, a model based on artificial neural network (ann) is presented for analyzing the ... | 2014 | 24291489 |
| σ(b) plays a limited role in the ability of listeria monocytogenes strain f2365 to survive oxidative and acid stress and in its virulence characteristics. | listeria monocytogenes strain f2365 was the first strain representative of serotype 4b (lineage i) to be sequenced in 2004, suggesting it could become the model organism for this serotype, which is associated with most human outbreaks of listeriosis worldwide to date. f2365 itself is an outbreak strain that was involved in the listeriosis outbreak associated with mexican-style soft cheese in california in 1985. in this study, we show through phenotypic and transcriptomic analysis that l. monocyt ... | 2013 | 24290686 |
| inhibition of listeria monocytogenes by propionic acid-based ingredients in cured deli-style turkey. | listeria monocytogenes growth can be controlled on ready-to-eat meats through the incorporation of antimicrobial ingredients into the formulation or by postlethality kill steps. however, alternate approaches are needed to provide options that reduce sodium content but maintain protection against pathogen growth in meats after slicing. the objective of this study was to determine the inhibition of l. monocytogenes by propionic acid-based ingredients in high-moisture, cured turkey stored at 4 or 7 ... | 2013 | 24290685 |
| transfer of foodborne pathogens during mechanical slicing and their inactivation by levulinic acid-based sanitizer on slicers. | this study investigated the degree of cross-contamination between deli foods and slicers by listeria monocytogenes, salmonella, and escherichia coli o157:h7, and their inactivation by levulinic acid (la) plus sodium dodecyl sulfate (sds) on slicers. the transfer rate of pathogens at 5 locations on the contaminated slicers (scenario i) and on food slices (scenario ii) was determined. the antimicrobial efficacy of the la + sds sanitizers applied either as a liquid or as foam at three concentration ... | 2014 | 24290650 |
| effectiveness of a bacteriophage in reducing listeria monocytogenes on fresh-cut fruits and fruit juices. | listeria monocytogenes is a serious foodborne pathogen and new strategies to control it in food are needed. among them, bacteriophages hold attributes that appear to be attractive. the objective of this study was to investigate the efficacy of the bacteriophage listex p100 to control l. monocytogenes growth on melon, pear and apple products (juices and slices) stored at 10 °c. l. monocytogenes grew well in untreated fruit slices. in juices, the pathogen grew in untreated melon, survived in untre ... | 2014 | 24290636 |
| modeling the growth of listeria monocytogenes on cut cantaloupe, honeydew and watermelon. | a recent outbreak linked to whole cantaloupes underscores the importance of understanding growth kinetics of listeria monocytogenes in cut melons at different temperatures. whole cantaloupe, watermelon, and honeydew purchased from a local supermarket were cut into 10 ± 1 g cubes. a four-strain cocktail of l. monocytogenes from food related outbreaks was used to inoculate fruit, resulting in ~10(3) cfu/10 g. samples were stored at 4, 10, 15, 20, or 25 °c and l. monocytogenes were enumerated at ap ... | 2014 | 24290625 |
| the polymyxin ceftazidime oxford medium as an alternative selective and differential medium for isolation of listeria monocytogenes from raw or unpasteurized food. | the polymyxin ceftazidime oxford medium (pcom) was developed to recover listeria monocytogenes from raw or unpasteurized foods. it contains esculin-ferric ammonium citrate as indicator system for listeria growth, and ceftazidime and polymyxin b as selective agents, which are available in several latin american countries. comparison of pcom, modified oxford medium (mox) and tryptic soy agar with 0.6% yeast extract (tsaye) indicated that both selective media were equally effective at recovering fo ... | 2014 | 24290624 |
| diminution of 2,3,5-triphenyltetrazolium chloride toxicity on listeria monocytogenes growth by iron source addition to the culture medium. | tetrazolium salts (ttz) such as 2,3,5-triphenyltetrazolium chloride (ttc) are readily reduced by bacterial populations of various genus. the reduced form of these redox indicators is conspicuously colored allowing a quick and easy detection of growth. the studies are mainly confined to gram negative bacteria because of an important toxic effect of tetrazolium salts on gram positive bacteria. indeed, we observed an important impact of different tetrazolium salts on listeria monocytogenes growth, ... | 2014 | 24290619 |
| listeria monocytogenes meningoencephalitis mimicking stroke in a patient with chronic lymphocytic leukemia. | listeria monocytogenes is an important opportunistic pathogen affecting patients with immunosuppression and shows a high tropism for the central nervous system. the clinical manifestations of central nervous system listerial infections are variable and represent a diagnostic challenge. | 2013 | 26000217 |
| crystal structure of the interferon gamma receptor alpha chain from chicken reveals an undetected extra helix compared with the human counterparts. | interferon gamma (ifn-γ) is an important cytokine that induces antiviral, antiproliferative, and immunomodulatory effects on target cells, and is also crucial in the early defense against intracellular parasites, such as listeria monocytogenes and toxoplasma gondii. the biological activity of ifn-γ relies upon the formation of a complex with its 2 receptors, the interferon gamma alpha chain (ifngr1) and beta chain (ifngr2), which are type ii cytokine receptors. structural models of ligand-recept ... | 2014 | 24283193 |
| determination of the relative level of detection of a qualitative microbiological measurement method with respect to a reference measurement method. | performance of qualitative microbiological measurement methods where the results are either "o" (microorganism not detected) or "1" (microorganism detected) is described by their probability of detection (pod) function, i.e., the pod as a function of the level of contamination of the sample, expressed as cfu/g or cfu/ml, or by the level of detection (lodp), i.e., the contamination of the sample that is detected (measurement result "1") with a specified probability p. when it is impossible to obt ... | 2013 | 24282952 |
| identification and partial characterization of a bacteriocin-like inhibitory substance (blis) from lb. bulgaricus k41 isolated from indigenous yogurts. | forty-two strains of lactobacillus bulgaricus isolated from locally made yogurts were examined and compared for bacteriocin producing ability using spot on lawn assay which improved by taking photo and image processing. lb. bulgaricus k41 exhibited the highest inhibition level against indicators. k41 bacteriocin-like inhibitory substance is sensitive to proteolytic enzymes (proteinase k, pepsin, and trypsin) but α-amylase makes slight reduction in its activity and it is resistant to lipase. this ... | 2014 | 24279356 |
| characterization of antioxidant-antibacterial quince seed mucilage films containing thyme essential oil. | in this study thyme essential oil (teo) concentrations ranging from 0% to 2.0%, incorporated in quince seed mucilage (qsm) film were used. antibacterial activity, physical, mechanical, barrier and antioxidant properties of qsm films were evaluated. the antimicrobial activity of the qsm films incorporated with thyme essential oil was screened against 11 important food-related bacterial strains by agar disc-diffusion assay. films containing 1% of thyme essential oil were effective against all test ... | 2014 | 24274540 |
| the ubiquitous nature of listeria monocytogenes clones: a large-scale multilocus sequence typing study. | listeria monocytogenes is ubiquitously prevalent in natural environments and is transmitted via the food chain to animals and humans, in whom it can cause life-threatening diseases. we used multilocus sequence typing (mlst) of ∼2000 isolates of l. monocytogenes to investigate whether specific associations existed between clonal complexes (ccs) and the environment versus diseased hosts. most ccs (72%) were not specific for any single source, and many have been isolated from the environment, food ... | 2014 | 24274459 |
| determination of listeria monocytogenes growth during mushroom production and distribution. | in the eu, food is considered safe with regard to listeria monocytogenes if its numbers do not exceed 100 cfu/g throughout the shelf-life of the food. therefore, it is important to determine if a food supports growth of l. monocytogenes. challenge studies to determine the ability of a food to support growth of l. monocytogenes are essential as predictive modelling often overestimates the growth ability of l. monocytogenes. the aim of this study was to determine if growth of l. monocytogenes was ... | 2013 | 28239137 |
| riboflavin (vitamin b2 ) deficiency impairs nadph oxidase 2 (nox2) priming and defense against listeria monocytogenes. | riboflavin, also known as vitamin b2 , is converted by riboflavin kinase (rfk) into flavin mononucleotide (fmn) and flavin adenine dinucleotide (fad), which are essential cofactors of dehydrogenases, reductases, and oxidases including the phagocytic nadph oxidase 2 (nox2). riboflavin deficiency is common in young adults and elderly individuals, who are at the coincidental risk for listeriosis. to address the impact of acute riboflavin deficiency on host defense against listeria monocytogenes (l. ... | 2014 | 24272050 |
| impacts of sample preparation methods on solubility and antilisterial characteristics of essential oil components in milk. | essential oil components (eocs) have limited water solubility and are used at much higher concentrations in complex food matrices than in growth media to inhibit pathogens. however, the correlation between solubility and activity has not been studied. the objective of this work was to characterize the solubility of eocs in solvents and milk and correlate solubility with antilisterial activity. the solubilities of four eocs, thymol, carvacrol, eugenol, and trans-cinnamaldehyde, in water was signi ... | 2014 | 24271170 |
| acquired resistance of listeria monocytogenes in and escaped from liver parenchymal cells to gentamicin is caused by being coated with their plasma membrane. | after systemic infection, a majority of listeria monocytogenes invade liver parenchymal cells (lpc), replicate therein and spread to neighboring cells, suggesting that 3 different types of l. monocytogenes exist in the liver: l. monocytogenes being unable to invade lpc, residing in lpc, and escaped from infected lpc. although listeriolysin o (llo) participates in escape of l. monocytogenes from macrophages and l. monocytogenes is susceptible to gentamicin (gm), it remains elusive whether llo par ... | 2014 | 24269768 |
| stevia rebaudiana bertoni as a natural antioxidant/antimicrobial for high pressure processed fruit extract: processing parameter optimization. | response surface methodology was used to evaluate the optimal high pressure processing treatment (300-500 mpa, 5-15 min) combined with stevia rebaudiana (stevia) addition (0-2.5% (w/v)) to guarantee food safety while maintaining maximum retention of nutritional properties. a fruit extract matrix was selected and listeria monocytogenes inactivation was followed from the food safety point of view while polyphenoloxidase (ppo) and peroxidase (pod) activities, total phenolic content (tpc) and antiox ... | 2014 | 24262555 |
| regulation of no synthesis, local inflammation, and innate immunity to pathogens by bet family proteins. | transcriptional activation of the nos2 gene, encoding inducible nitric oxide synthase (inos), during infection or inflammation requires coordinate assembly of an initiation complex by the transcription factors nf-κb and type i interferon-activated isgf3. here we show that infection of macrophages with the intracellular bacterial pathogen listeria monocytogenes caused binding of the bet proteins brd2, brd3, and, most prominently, brd4 to the nos2 promoter and that a profound reduction of nos2 exp ... | 2014 | 24248598 |
| beneficial innate signaling interference for antibacterial responses by a toll-like receptor-mediated enhancement of the mkp-irf3 axis. | a major function of innate immune receptors is to recognize pathogen-associated molecular patterns and then evoke immune responses appropriate to the nature of the invading pathogen(s). because innate immune cells express various types of these receptors, distinct combinations of signaling pathways are activated in response to a given pathogen. although the conventional wisdom is that these signaling pathways cooperate with one another to ensure an effective host response, a more nuanced view re ... | 2013 | 24248350 |
| [infective endocarditis caused by listeria monocytogenes: review of the literature and a case report]. | infective endocarditis caused by listeria monocytogenesis an extremely rare but usually aggressive disease. we have seen in recent years an increase in age of onset of this disease and a predilection for patients with valvular disease, especially prosthetic valve. the treatment of choice is ampicillin, which is combined with gentamicin for synergy. it is known that even with treatment the mortality is very high and higher than in other types of bacterial endocarditis. with optimal therapy, that ... | 2013 | 24248114 |
| [infections by listeria monocytogenes]. | listeria monocytogenes infections have been poorly characterized in chile. | 2013 | 24248112 |
| [main virulence factors of listeria monocytogenes and its regulation]. | listeria monocytogenesis a facultative intracellular pathogen, ubiquitous and aetiological agent of listeriosis. the main way of acquisition is the consumption of contaminated food and can cause serious medical conditions such as septicemia, meningitis and gastroenteritis, especially in children, immunocompromised individuals and seniors and abortions in pregnant women. an increase in cases of listeriosis worldwide has been reported and it is estimated that its prevalence in developed countries ... | 2013 | 24248111 |
| [listeria monocytogenes]. | | 2013 | 24248110 |
| post-parturient shedding of listeria monocytogenes in breast milk of infected mice. [corrected]. | the objectives of this study were to develop an animal model to study listeria monocytogenes infection during the peri-parturient period and identify sources of maternal shedding of the pathogen. peri-parturient mice were infected intragastrically with l. monocytogenes that expressed bacterial luciferase. mice were then imaged in vivo over time. secreted breast milk samples from mice infected after parturition were enriched and plated for culture and imaging. bioluminescence imaging technology w ... | 2013 | 24246517 |
| fascin confers resistance to listeria infection in dendritic cells. | ag-presenting dendritic cells (dcs) must survive bacterial infection to present ag information to naive t cells. the greater ability of dcs' host defense is evident from the report that dcs are more resistant to listeria monocytogenes than macrophages. however, the molecular mechanism of this resistance is unclear. we found that listeria replicate more slowly in wild-type dcs compared with fascin1 knockout dcs. this finding is significant because fascin1, an actin-bundling protein, is specifical ... | 2013 | 24244012 |
| the tuberculosis vaccine candidate bacillus calmette-guérin δurec::hly coexpressing human interleukin-7 or -18 enhances antigen-specific t cell responses in mice. | bacillus calmette-guérin (bcg), the only approved tuberculosis vaccine, provides only limited protection. previously, we generated a recombinant derivative (bcg δurec::hly), which secretes the pore-forming toxin listeriolysin o (llo) of listeria monocytogenes. this vaccine shows superior protection against tuberculosis in preclinical models and is safe in humans. here we describe two new vaccine strains which express human interleukin-7 (hil)-7 or hil-18 in the genetic background of bcg δurec::h ... | 2013 | 24236077 |
| lytic bacteriophages: potential interventions against enteric bacterial pathogens on produce. | foodborne illnesses resulting from the consumption of produce commodities contaminated with enteric pathogens continue to be a significant public health issue. lytic bacteriophages may provide an effective and natural intervention to reduce bacterial pathogens on fresh and fresh-cut produce commodities. the use of multi-phage cocktails specific for a single pathogen has been most frequently assessed on produce commodities to minimize the development of bacteriophage insensitive mutants (bim) in ... | 2013 | 24228223 |
| temporal requirements for b cells in the establishment of cd4 t cell memory. | cd4 t cell memory generation is shaped by a number of factors, including the strength and duration of tcr signaling, as well as the priming environment, all of which can be modified by b cells. studies using b cell-deficient mice indicate b cells play a critical role in generating effector and memory cd4 t cells; however, when and how b cells are acting to promote these responses has not yet been ascertained. in this study, we use anti-cd20 ab depletion of b cells at different times following li ... | 2013 | 24218454 |
| use of germicidal uv light to reduce low numbers of listeria monocytogenes on raw chicken meat. | listeria monocytogenes is a common constituent of the microbiological community in poultry processing plants and can be found in low numbers on raw poultry. raw meat is the most important source of this pathogen in commercial cooking facilities. germicidal uv light was tested as a means to kill l. monocytogenes inoculated onto broiler breast fillets. treatments at 800 μw/ cm(2) for 5 s to 5 min of exposure were tested against inocula of 35 to 60 cells per fillet. all fillets were sampled by rins ... | 2013 | 24215703 |
| mathematical models to predict kinetic behavior and growth probabilities of listeria monocytogenes on pork skin at constant and dynamic temperatures. | in this study, mathematical models were developed to predict the growth probability and kinetic behavior of listeria monocytogenes on fresh pork skin during storage at different temperatures. a 10-strain mixture of l. monocytogenes was inoculated on fresh pork skin (3 by 5 cm) at 4 log cfu/cm(2). the inoculated samples were stored aerobically at 4, 7, and 10 °c for 240 h, at 15 and 20 °c for 96 h, and at 25 and 30 °c for 12 h. the baranyi model was fitted to l. monocytogenes growth data on palca ... | 2013 | 24215689 |
| listeria monocytogenes in five sardinian swine slaughterhouses: prevalence, serotype, and genotype characterization. | in a 3-year study (2008 to 2011) to estimate the prevalence and the contamination sources of listeria monocytogenes in pork meat in sardinia, italy, 211 samples were collected from five sardinian swine slaughterhouses: 171 samples from slaughtered pigs and 40 from the slaughterhouse environment. fifty l. monocytogenes isolates were characterized by pcr-based serotyping, presence of virulence-associated genes, and pulsed-field gel electrophoresis restriction analysis. the overall prevalence of l. ... | 2013 | 24215688 |
| pickled egg production: inactivation rate of salmonella, escherichia coli o157:h7, listeria monocytogenes, and staphylococcus aureus during acidification step. | based on current u.s. food and drug administration acidified foods guidelines, regulatory approval of commercial pickled egg production without a final heat treatment requires challenge studies. we conducted challenge studies to verify common pickled egg processing parameters. hard-boiled eggs were acidified in ambient temperature brine at a 60:40 egg/brine ratio. four acidification treatments were studied in triplicate: 5% acetic acid (aa) or 2.5% aa brine with and without 0.05% sodium benzoate ... | 2013 | 24215686 |
| btla interaction with hvem expressed on cd8(+) t cells promotes survival and memory generation in response to a bacterial infection. | the b and t lymphocyte attenuator (btla) is an ig super family member that binds to the herpes virus entry mediator (hvem), a tnf receptor super family (tnfrsf) member. engagement of btla by hvem triggers inhibitory signals, although recent evidence indicates that btla also may act as an activating ligand for hvem. in this study, we reveal a novel role for the btla-hvem pathway in promoting the survival of activated cd8(+) t cells in the response to an oral microbial infection. our data show tha ... | 2013 | 24205057 |
| identification of tlr10 as a key mediator of the inflammatory response to listeria monocytogenes in intestinal epithelial cells and macrophages. | listeria monocytogenes is a gram-positive bacterium that can cause septicemia and meningitis. tlrs are central receptors of the innate immune system that drive inflammatory responses to invading microbes such as l. monocytogenes. although intestinal epithelial cells (iecs) represent the initial point of entry used by l. monocytogenes for infection, the innate immune response to l. monocytogenes in these cells has been poorly characterized to date. the aim of this study was to determine which tlr ... | 2013 | 24198280 |
| [resistance of listeria monocytogenes in simulated gastrointestinal systems]. | resistance of listeria monocytogenes was determined through its survival rate and intracellular ph (ph(i)) after exposed to in vitro digestion models. | 2013 | 24195384 |
| crystallization and x-ray crystallographic analysis of the cholesterol-dependent cytolysin listeriolysin o from listeria monocytogenes. | the secreted pore-forming toxin listeriolysin o (llo) from the intracellular pathogen listeria monocytogenes is a member of the family of cholesterol-dependent cytolysins (cdc) with broad properties in pathogenesis. its role as a virulence factor is enigmatic: it disrupts membranes and acts as an inductor of both pro- and anti-inflammatory responses in infected cells. in addition, llo is also a potent target for immunogenicity during infection. natively secreted llo from a recombinant l. innocua ... | 2013 | 24192351 |
| listeriosis during pregnancy: a public health concern. | listeria was first described in 1926 by murray, webb, and swann, who discovered it while investigating an epidemic infection among laboratory rabbits and guinea pigs. the role of listeria monocytogenes as a foodborne pathogen was definitively recognized during the 1980s. this recognition was the consequence of a number of epidemic human outbreaks due to the consumption of contaminated foods, in canada, in the usa and in europe. listeriosis is especially severe in immunocompromised individuals su ... | 2013 | 24191199 |
| assessing in-house monitoring efficiency by tracing contamination rates in cheese lots recalled during an outbreak of listeriosis in austria. | a cluster of 34 cases of listeriosis was traced to consumption of quargel cheese, a sour milk specialty, in austria, germany and czech republic between 2009 and 2010. after recall from the retail market all soft cheese batches (n = 18) were sent for investigation and iso 11290 based microbiological analysis revealed all red smear-ripened batches (16/18) to be positive for listeria monocytogenes whereas mold ripened cheeses were negative. the 16 positive batches were grouped into three categories ... | 2013 | 24184614 |
| strain variability of the behavior of foodborne bacterial pathogens: a review. | differences in phenotypic responses among strains of the same microbial species constitute an important source of variability in microbiological studies, and as such they need to be assessed, characterized and taken into account. this review provides a compilation of available research data on the strain variability of four basic behavioral aspects of foodborne bacterial pathogens including: (i) virulence; (ii) growth; (iii) inactivation; and (iv) biofilm formation. a particular emphasis is plac ... | 2013 | 24184609 |
| foci of contamination of listeria monocytogenes in different cheese processing plants. | listeria monocytogenes is a ubiquitous bacterium widely distributed in the environment that can cause a severe disease in humans when contaminated foods are ingested. cheese has been implicated in sporadic cases and in outbreaks of listeriosis worldwide. environmental contamination, in several occasions by persistent strains, has been considered an important source of finished product contamination. the objectives of this research were to (i) evaluate the presence of l. monocytogenes within the ... | 2013 | 24184608 |
| listeria monocytogenes bacteremia and cmv colitis in a patient with ulcerative colitis. | | 2014 | 24183232 |
| prevalence and characteristics of listeria monocytogenes in feces of black beef cattle reared in three geographically distant areas in japan. | this study was conducted to determine the prevalence and characteristics of listeria monocytogenes in the feces of black beef cattle reared in geographically distant areas in japan. we surveyed 130 farms in the following three areas: northern (hokkaido prefecture), central (gifu and mie prefectures), and southern (oita, miyazaki, and kagoshima prefectures) areas and collected 1738 fecal samples. our data showed the following isolation rate for each area: northern, 11.4% of 651; central, 2.8% of ... | 2014 | 24180587 |
| illuminating the petite picture of t cell memory responses to listeria monocytogenes. | the ease to culture, moderately less safety constraints in handling, and above all, hurdle free induction of an anticipated infection in mouse rendered listeria monocytogenes the rank of a model organism for studying a variety of host immune responses. listeria monocytogenes being an intracellular pathogen evokes potent cd8 t cell response during which cd8 t cells pass through a massive expansion phase. this is generally followed by contraction phase wherein majority of activated cells undergo a ... | 2013 | 24171157 |
| sensitivity analysis for critical control points determination and uncertainty analysis to link fso and process criteria: application to listeria monocytogenes in soft cheese made from pasteurized milk. | microbiological food safety is an important economic and health issue in the context of globalization and presents food business operators with new challenges in providing safe foods. the hazard analysis and critical control point approach involve identifying the main steps in food processing and the physical and chemical parameters that have an impact on the safety of foods. in the risk-based approach, as defined in the codex alimentarius, controlling these parameters in such a way that the fin ... | 2014 | 24168722 |
| [analysis of the microbiological quality and potential presence of listeria monocytogenes in custard apple (annona muricata), mango (mangifera indica) and passion fruit (passiflora edulis) pulps from costa rica]. | the objective of this work was to determine some of the indicators associated to shelf life, hygiene, process and storage conditions for some of custard apple, mango and passion fruit pulps distributed by the main supermarket chains of the metropolitan area of san josé, costa rica, as well as to examine the potential presence of listeria monocytogenes in them. sixty fruit pulp samples were analyzed. tests included ph determination, total aerobic plate count, yeasts and mold count, lactic bacteri ... | 2013 | 24167958 |
| nod1 and nod2 enhance tlr-mediated invariant nkt cell activation during bacterial infection. | invariant nkt (inkt) cells act at the crossroad between innate and adaptive immunity and are important players in the defense against microbial pathogens. inkt cells can detect pathogens that trigger innate receptors (e.g., tlrs, rig-i, dectin-1) within apcs, with the consequential induction of cd1d-mediated ag presentation and release of proinflammatory cytokines. we show that the cytosolic peptidoglycan-sensing receptors nod1 and nod2 are necessary for optimal ifn-γ production by inkt cells, a ... | 2013 | 24163408 |
| confirmed low prevalence of listeria mastitis in she-camel milk delivers a safe, alternative milk for human consumption. | she-camel milk is an alternative solution for people allergic to milk; unfortunately, potential harmful bacteria have not been tested in she-camel milk. listeria monocytogenes is one harmful bacterium that causes adverse health effects if chronically or acutely ingested by humans. the purpose of this study was to estimate the prevalence, characterize the phenotypic, genetic characterization, virulence factors, and antibiopotential harmful bacteria resistance profile of listeria isolated from the ... | 2014 | 24161878 |