| listeria monocytogenes infection in a sugar glider (petaurus breviceps) - new mexico, 2011. | listeria monocytogenes is a gram-positive, facultative anaerobic, rod-shaped bacterium that can infect and cause disease in many species. in this case report, we describe a case of l. monocytogenes infection causing sepsis in a sugar glider (petaurus breviceps). the sugar glider consumed a varied diet consisting of human food items, including cantaloupe. a nationwide outbreak of l. monocytogenes foodborne illness associated with cantaloupes occurred simultaneously with this incident case. in thi ... | 2015 | 24815369 |
| cytosolic double-stranded dna induces nonnecroptotic programmed cell death in trophoblasts via ifi16. | the mechanisms underlying the immune defense by trophoblasts against pathogens remain ill defined. we demonstrated that placental cell death was increased upon in vivo exposure to listeria monocytogenes. the death of infected cells is an important host innate defense mechanism. meanwhile, double-stranded dna (dsdna) derived from intracellular bacteria or dsdna viruses is emerging as a potent pathogen-associated molecular pattern recognized by host cells. we sought to characterize trophoblast dea ... | 2014 | 24812048 |
| how listeria monocytogenes organizes its surface for virulence. | listeria monocytogenes is a gram-positive pathogen responsible for the manifestation of human listeriosis, an opportunistic foodborne disease with an associated high mortality rate. the key to the pathogenesis of listeriosis is the capacity of this bacterium to trigger its internalization by non-phagocytic cells and to survive and even replicate within phagocytes. the arsenal of virulence proteins deployed by l. monocytogenes to successfully promote the invasion and infection of host cells has b ... | 2014 | 24809022 |
| low-fat frankfurters from protein concentrates of tilapia viscera and mechanically separated tilapia meat. | in order to develop a healthy low-fat frankfurter-type sausage, different formulations were developed with tilapia viscera surimi (t1) and two with mechanically separated tilapia meat (mstm) surimi (t2 and t3), all without pig lard addition. due to technological problems observed for t1 sausage during cooking, it was not further investigated. the functionality of the other two formulations was evaluated based on proximate composition, ph, water activity, and texture. finally, microbiological and ... | 2013 | 24804055 |
| rapid differentiation of listeria monocytogenes epidemic clones iii and iv and their intact compared with heat-killed populations using fourier transform infrared spectroscopy and chemometrics. | the objectives of this study were to determine if fourier transform infrared (ft-ir) spectroscopy and multivariate statistical analysis (chemometrics) could be used to rapidly differentiate epidemic clones (ecs) of listeria monocytogenes, as well as their intact compared with heat-killed populations. ft-ir spectra were collected from dried thin smears on infrared slides prepared from aliquots of 10 μl of each l. monocytogenes ecs (eciii: j1-101 and r2-499; eciv: j1-129 and j1-220), and also from ... | 2014 | 24802119 |
| temperature- and nitrogen source-dependent regulation of glnr target genes in listeria monocytogenes. | the ubiquitous pathogen listeria monocytogenes lives either saprophytically in the environment or within cells in a vertebrate host, thus adapting its lifestyle to its ecological niche. growth experiments at 24 and 37 °c (environmental and host temperature) with ammonium or glutamine as nitrogen sources revealed that ammonium is the preferred nitrogen source of l. monocytogenes. reduced growth on glutamine is more obvious at 24 °c. global transcriptional microarray analyses showed that the most ... | 2014 | 24801548 |
| trial watch: dna vaccines for cancer therapy. | during the past 2 decades, the possibility that preparations capable of eliciting tumor-specific immune responses would mediate robust therapeutic effects in cancer patients has received renovated interest. in this context, several approaches to vaccinate cancer patients against their own malignancies have been conceived, including the administration of dna constructs coding for one or more tumor-associated antigens (taas). such dna-based vaccines conceptually differ from other types of gene the ... | 2014 | 24800178 |
| biochemical and antimicrobial activity of phloretin and its glycosilated derivatives present in apple and kumquat. | phloretin and its glycosylated derivatives (phlorizin and phloretin 3',5'-di-c-glucoside) are dihydrochalcones that have many interesting biological properties. the results obtained showed that the dihydrochalcones are able to inhibit growth of gram positive bacteria, in particular staphylococcus aureus atcc 6538, listeria monocytogenes atcc 13932 and methicillin-resistant s. aureus clinical strains. moreover, phloretin is active also against the gram negative bacteria salmonella typhimurium atc ... | 2014 | 24799241 |
| multifaceted activity of listeriolysin o, the cholesterol-dependent cytolysin of listeria monocytogenes. | the cholesterol-dependent cytolysins (cdcs) are a large family of pore-forming toxins that are produced by numerous gram-positive bacterial pathogens. these toxins are released in the extracellular environment as water-soluble monomers or dimers that bind to cholesterol-rich membranes and assemble into large pore complexes. depending upon their concentration, the nature of the host cell and membrane (cytoplasmic or intracellular) they target, the cdcs can elicit many different cellular responses ... | 2014 | 24798012 |
| phenotypic t cell exhaustion in a murine model of bacterial infection in the setting of pre-existing malignancy. | while much of cancer immunology research has focused on anti-tumor immunity both systemically and within the tumor microenvironment, little is known about the impact of pre-existing malignancy on pathogen-specific immune responses. here, we sought to characterize the antigen-specific cd8+ t cell response following a bacterial infection in the setting of pre-existing pancreatic adenocarcinoma. mice with established subcutaneous pancreatic adenocarcinomas were infected with listeria monocytogenes, ... | 2014 | 24796533 |
| segmented continuous-flow multiplex polymerase chain reaction microfluidics for high-throughput and rapid foodborne pathogen detection. | high-throughput and rapid identification of multiple foodborne bacterial pathogens is vital in global public health and food industry. to fulfill this need, we propose a segmented continuous-flow multiplex polymerase chain reaction (scf-mpcr) on a spiral-channel microfluidic device. the device consists of a disposable polytetrafluoroethylene (ptfe) capillary microchannel coiled on three isothermal blocks. within the channel, n segmented flow regimes are sequentially generated, and m-plex pcr is ... | 2014 | 24793853 |
| oral infection drives a distinct population of intestinal resident memory cd8(+) t cells with enhanced protective function. | the intestinal mucosa promotes t cell responses that might be beneficial for effective mucosal vaccines. however, intestinal resident memory t (trm) cell formation and function are poorly understood. we found that oral infection with listeria monocytogenes induced a robust intestinal cd8 t cell response and blocking effector t cell migration showed that intestinal trm cells were critical for secondary protection. intestinal effector cd8 t cells were predominately composed of memory precursor eff ... | 2014 | 24792910 |
| vaccination studies: detection of a listeria monocytogenes-specific t cell immune response using the elispot technique. | during systemic infection by listeria monocytogenes the host develops a robust t cell-mediated immune response against the major immunodominant antigens of the pathogen. the enzyme-linked immuno-spot (elispot) test is an accurate and reproducible means of measuring the extent of this t cell response. here we describe a detailed elispot protocol for measuring an epitope-specific cd8+ t cell-mediated immune response in mice vaccinated with low doses of l. monocytogenes. the basic approach can be e ... | 2014 | 24792565 |
| control of listeria monocytogenes in the processing environment by understanding biofilm formation and resistance to sanitizers. | listeria monocytogenes can colonize in the food processing environment and thus pose a greater risk of cross-contamination to food. one of the proposed mechanisms that facilitates such colonization is biofilm formation. as part of a biofilm, it is hypothesized that l. monocytogenes can survive sanitization procedures. in addition, biofilms are difficult to remove and may require additional physical and chemical mechanisms to reduce their presence and occurrence. the initial stage of biofilm form ... | 2014 | 24792564 |
| genome sequencing of listeria monocytogenes. | genome sequencing is a key technology in microbiology. a genome sequence is the prerequisite for understanding the molecular basis of a given phenotype; this is of particular importance for pathogens. particularly for the foodborne pathogen listeria monocytogenes, which is an important model organism in infection biology, genome sequencing has proven to be invaluable in advancing our understanding of its virulence mechanisms and epidemiology. in this chapter, current technologies and software to ... | 2014 | 24792562 |
| absolute and relative gene expression in listeria monocytogenes using real-time pcr. | reverse transcription combined with the polymerase chain reaction (rt-pcr) is a viable method widely used to quantify gene expression. there are two ways to quantify gene expression by real-time pcr: relative quantification and absolute quantification. relative quantification relates the pcr signal of the target gene to a control gene, normally a housekeeping gene. absolute quantification determines the input copy number, usually by relating the pcr signal to a standard curve. here we describe b ... | 2014 | 24792561 |
| mutant construction and integration vector-mediated gene complementation in listeria monocytogenes. | genes that play role in stress response mechanisms and other phenotypes of bacteria can be identified by construction and screening of mutant libraries. in this chapter, we describe the construction and screening of mutant libraries of listeria monocytogenes using a plasmid, pmc38, carrying a mariner-based transposon system (tc1/mariner) and constructed by cao et al. (appl environ microbiol 73:2758-2761, 2007). following screening of the mutant library, putative mutants are identified and the tr ... | 2014 | 24792560 |
| generation of nonpolar deletion mutants in listeria monocytogenes using the "soeing" method. | the ability to manipulate chromosomally encoded genes is a fundamental biological tool for the analysis of gene function. here, we provide in greater depth protocols for the creation of nonpolar unlabeled gene deletions in listeria (l.) monocytogenes that are facilitated by the splicing overlap extension pcr technique. for mutagenesis in l. monocytogenes, we describe two different plasmid-based approaches, which facilitate the introduction of this spliced amplicon in place of the corresponding s ... | 2014 | 24792559 |
| extraction and analysis of plasmid dna from listeria monocytogenes. | a plasmid preparation is a method used to extract and purify plasmid dna. methods developed to purify plasmid dna from bacteria generally involve harvesting and alkaline lysis of the bacteria and precipitation of chromosomal dna and protein, followed by purification of the plasmid dna. here, we describe the mini-preparation of plasmid dna by a rapid small-scale method, adapted for listeria monocytogenes. the quality of plasmid dna isolated using this method is sufficient for analytical purposes ... | 2014 | 24792558 |
| internalization assays for listeria monocytogenes. | listeria monocytogenes is a model intracellular pathogen that can invade the cytoplasm of host mammalian cells. cellular invasion can be measured using standard techniques such as the classical gentamicin protection assay, based on the quantification of colony-forming units from lysates of infected cells. in addition, there are methods based on immunofluorescence microscopy which allow for assaying invasion in a medium- to high-throughput manner. in the following sections we detail two different ... | 2014 | 24792557 |
| virulence characterization of listeria monocytogenes. | listeria monocytogenes, a foodborne intracellular pathogen, is responsible for about 300 deaths every year in the usa. it has the ability to escape host defense mechanisms and causes listeriosis more frequently in immunocompromised individuals. virulence mechanisms in l. monocytogenes are highly regulated and tightly controlled. a number of virulence factors that play important roles in pathogenesis of listeriosis have been identified and characterized. this review highlights the power of compar ... | 2014 | 24792556 |
| the listeria cell wall and associated carbohydrate polymers. | understanding molecular interactions of bacteria with their environment requires the purification and characterization of cell wall components. here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (wta), which assume important roles as major constituents of gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages. specifically, we present a procedure for c ... | 2014 | 24792554 |
| analysis of listeria monocytogenes subproteomes. | the proteomic approaches have considerably evolved over the past two decades. this opened the doors for larger scale and deeper explorations of cellular physiology. like for other living organisms, using the tools of proteomics has undoubtedly improved knowledge about the foodborne pathogen listeria monocytogenes. among the different technologies and approaches permanently evolving in the field of proteomics, the 2-de is an analytical separation method of choice to resolve thousands of proteins ... | 2014 | 24792553 |
| high-throughput characterization of listeria monocytogenes using the omnilog phenotypic microarray. | high-throughput biochemical screening techniques are an important tool in phenotypic analysis of bacteria. new methods, simultaneously measuring many phenotype responses, increase the output of such investigations and allow a more complete overview of the bacterial phenotype, and how this may relate its genotype. this chapter describes the application of omnilog phenotype microarray analysis, a high-throughput assay for the phenotypic characterization of bacterial strains across a variety of dif ... | 2014 | 24792552 |
| fluorescent amplified fragment length polymorphism (faflp) analysis of listeria monocytogenes. | fluorescent amplified fragment length polymorphism (faflp) is based on the selective pcr amplification of restriction fragments from a digest of total genomic dna. genomic dna extracted from a purified bacterial isolate is completely digested with two endonucleases generating fragments which are ligated to specific double-stranded adaptors. the ligated fragments are then amplified by pcr using fluorescently labelled primers. fluorescent amplified fragments are separated by size on an automated s ... | 2014 | 24792551 |
| ribotyping and automated ribotyping of listeria monocytogenes. | ribotyping is a molecular technique that allows identification and typing of bacteria to the strain level. it is based on restriction endonuclease cleavage of total genomic dna followed by electrophoretic separation, southern blot transfer, and hybridization of transferred dna fragments with a radiolabeled ribosomal operon probe. following autoradiography, only those bands containing a portion of the ribosomal operon are visualized. the number of fragments generated by ribotyping is a reflection ... | 2014 | 24792550 |
| multilocus sequence typing (mlst) of listeria monocytogenes. | nucleotide sequence-based methods focusing on the single nucleotide polymorphisms (snps) of listeria monocytogenes housekeeping genes facilitate the rapid and interlaboratory comparison on open accessible databases, such as the multilocus sequence typing (mlst) databases that are available. mlst has advantages over other methods as it can reconstruct ancestral and evolutionary linkage between l. monocytogenes isolates. mlst detects all genetic variations within the amplified housekeeping gene th ... | 2014 | 24792549 |
| pulsed-field gel electrophoresis (pfge) analysis of listeria monocytogenes. | pfge is a valuable tool for assessing listeria monocytogenes strain interrelatedness. it is based on the study of total bacterial dna restriction patterns. cells are embedded in agarose plugs before being lysed. the released dna is then digested into large fragments by restriction enzymes. as dna fragments are too large to be separated by traditional electrophoresis in an agarose gel, changes in the direction of the electrical current are periodically applied in order to allow the proper migrati ... | 2014 | 24792548 |
| serotype assignment by sero-agglutination, elisa, and pcr. | for assessing isolates of listeria monocytogenes serotype designation is the foremost subtyping method used. traditionally, serotyping has been done with agglutination reactions. in the last decade, alternative serotyping methods were described using enzyme-linked immunosorbent assay (elisa) and polymerase chain reaction (pcr). herein are described the three methods, and the advantages and disadvantages of each. | 2014 | 24792547 |
| traditional methods for isolation of listeria monocytogenes. | conventional methods for the detection of listeria monocytogenes in foods and environmental samples relies on selective pre-enrichment, enrichment, and plating. this is followed by confirmation of suspected colonies by testing a limited number of biochemical markers. | 2014 | 24792545 |
| isolation of listeria monocytogenes from food and water: official and experimental protocols. | listeria monocytogenes is frequently encountered in foods but often at low concentrations and typically in the presence of other microbiota, including nonpathogenic listeria spp. the potential of l. monocytogenes to cause severe human disease mandates sensitive, accurate, and rapid detection in foods. isolation of l. monocytogenes from foods is critical, not only for routine surveillance, but also for epidemiologic investigations. isolation of the pathogen from water (especially surface water us ... | 2014 | 24789600 |
| identification of the significant factors in food safety using global sensitivity analysis and the accept-and-reject algorithm: application to the cold chain of ham. | deterministic models describing heat transfer and microbial growth in the cold chain are widely studied. however, it is difficult to apply them in practice because of several variable parameters in the logistic supply chain (e.g., ambient temperature varying due to season and product residence time in refrigeration equipment), the product's characteristics (e.g., ph and water activity) and the microbial characteristics (e.g., initial microbial load and lag time). this variability can lead to dif ... | 2014 | 24786551 |
| infective endocarditis caused by listeria monocytogenes forming a pseudotumor. | a 73-year-old woman with breast cancer and metastasis under chemotherapy suffered from fever, pleural effusion and pericardial effusion. despite the administration of treatment with cefozopran and prednisolone, the patient's fever relapsed. an electrocardiogram identified a new complete atrioventricular block and an echocardiogram revealed vegetation with an unusual pseudotumoral mass in the right atrium. blood cultures grew listeria monocytogenes. the patient was eventually diagnosed with right ... | 2014 | 24785898 |
| development of performance objectives for listeria monocytogenes contaminated salmon (salmo salar) intended used as sushi and sashimi based on analyses of naturally contaminated samples. | raw salmon is commonly used in sushi and sashimi, indicating that fresh salmon may be considered as a ready-to-eat product. listeria monocytogenes is occasionally present in fresh salmon, but studies of prevalence and growth of the bacterium in this matrix have been few. in the present study, salmon from a company where l. monocytogenes is present in low levels has been investigated in order to develop performance objectives for l. monocytogenes in fresh salmon intended for use in ready-to-eat p ... | 2014 | 24784883 |
| survival of listeria monocytogenes on fresh blueberries (vaccinium corymbosum) stored under controlled atmosphere and ozone. | listeria monocytogenes is a foodborne pathogen that represents a high risk for consumers because it can grow under refrigeration conditions and can also develop acid tolerance. fresh blueberries are hand-picked, packed, and transported under refrigeration without receiving a microbial inactivation treatment. the aim of this work was to study the survival of l. monocytogenes in fresh highbush blueberries stored at 4 or 12 °c under different controlled atmosphere conditions, including air (control ... | 2014 | 24780341 |
| evaluation of the thin agar layer method for the recovery of pressure-injured and heat-injured listeria monocytogenes. | a sublethally injured bacterial cell has been defined as a cell that survives a stress such as heating, freezing, acid treatment, or other antimicrobial intervention but can repair the cellular damage exerted by the stressor and later regain its original ability to grow. consequently, sublethally injured cells are not likely to be included in conventional enumeration procedures, which could result in unrealistically low counts unless efforts are made to encourage recovery of the injured cells be ... | 2014 | 24780340 |
| effects of different nitrite concentrations from a vegetable source with and without high hydrostatic pressure on the recovery of listeria monocytogenes on ready-to-eat restructured ham. | sodium nitrite exerts an inhibitory effect on the growth of listeria monocytogenes. the objective of this study was to investigate the effects of various nitrite concentrations from a vegetable source with and without high hydrostatic pressure (hhp) on the recovery and growth of l. monocytogenes on ready-to-eat restructured ham. a preconverted celery powder was used as the vegetable source of nitrite. targeted concentrations of natural nitrite investigated were 0, 50, and 100 mg/kg. hhp treatmen ... | 2014 | 24780333 |
| delivery of selective agents via time-delayed release tablets improves recovery of listeria monocytogenes injured by acid and nitrite. | listeria selective enrichment media are designed to enhance the isolation of the organism and increase the chances of detection. drawbacks include the requirements for prolonged sample incubation (48 to 72 h) and manual addition of selective agents, which may be a source of contamination. modified listeria recovery broth (mlrb) is a proprietary enrichment medium formulated to facilitate the recovery of injured cells; its selective agents are incorporated into a format that allows delayed release ... | 2014 | 24780332 |
| listeria monocytogenes transfer during mechanical dicing of celery and growth during subsequent storage. | the transfer of listeria monocytogenes to previously uncontaminated product during mechanical dicing of celery and its growth during storage at various temperatures were evaluated. in each of three trials, 275 g of retail celery stalks was immersed in an aqueous five-strain l. monocytogenes cocktail to obtain an average of 5.6 log cfu/g and then was diced using a hand-operated dicer, followed by sequential dicing of 15 identical 250-g batches of uninoculated celery using the same dicer. each bat ... | 2014 | 24780331 |
| responding to bioterror concerns by increasing milk pasteurization temperature would increase estimated annual deaths from listeriosis. | in a 2005 analysis of a potential bioterror attack on the food supply involving a botulinum toxin release into the milk supply, the authors recommended adopting a toxin inactivation step during milk processing. in response, some dairy processors increased the times and temperatures of pasteurization well above the legal minimum for high temperature, short time pasteurization (72 °c for 15 s), with unknown implications for public health. the present study was conducted to determine whether an inc ... | 2014 | 24780323 |
| longitudinal requirement for cd4+ t cell help for adenovirus vector-elicited cd8+ t cell responses. | despite the widespread use of replication-incompetent recombinant adenovirus (ad) vectors as candidate vaccine platforms, the mechanism by which these vectors elicit cd8(+) t cell responses remains poorly understood. our data demonstrate that induction and maintenance of cd8(+) t cell responses by ad vector immunization is longitudinally dependent on cd4(+) t cell help for a prolonged period. depletion of cd4(+) t cells in wild type mice within the first 8 d following ad immunization resulted in ... | 2014 | 24778441 |
| the drosophila deubiquitinating enzyme dusp36 acts in the hemocytes for tolerance to listeria monocytogenes infections. | listeria monocytogenes is a facultative intracellular pathogen which can infect drosophila melanogaster. upon infection, drosophila mounts an immune response including antimicrobial peptide production and autophagy activation. a set of previously published results prompted us to study the role of the deubiquitinating enzyme dusp36 in response to l. monocytogenes infections. we show in this report that flies with dusp36-specific inactivation in hemocytes are susceptible to l. monocytogenes infect ... | 2014 | 24777180 |
| ox40 controls effector cd4+ t-cell expansion, not follicular t helper cell generation in acute listeria infection. | to investigate the importance of ox40 signals for physiological cd4(+) t-cell responses, an endogenous antigen-specific population of cd4(+) t cells that recognise the 2w1s peptide was assessed and temporal control of ox40 signals was achieved using blocking or agonistic antibodies (abs) in vivo. following infection with listeria monocytogenes expressing 2w1s peptide, ox40 was briefly expressed by the responding 2w1s-specific cd4(+) t cells, but only on a subset that co-expressed effector cell m ... | 2014 | 24771127 |
| investigating the control of listeria monocytogenes on alternatively-cured frankfurters using natural antimicrobial ingredients or post-lethality interventions. | the objective of this study was to investigate natural antimicrobials including cranberry powder, dried vinegar and lemon juice/vinegar concentrate, and post-lethality interventions (lauric arginate, octanoic acid, thermal treatment and high hydrostatic pressure) for the control of listeria monocytogenes on alternatively-cured frankfurters. lauric arginate, octanoic acid, and high hydrostatic pressure (400 mpa) reduced l. monocytogenes populations by 2.28, 2.03, and 1.88 log 10 cfu per g compare ... | 2014 | 24769878 |
| presence and molecular characterization of the major serovars of listeria monocytogenes in ten sardinian fermented sausage processing plants. | the aim of the present study was to investigate the occurrence of listeria monocytogenes in ten sardinian fermented sausage processing plants. a total of 230 samples were collected and 40 l. monocytogenes isolates were obtained and subjected to serotyping and investigated for the presence of ten virulence-associated genes using multiplex pcr assays. the isolates were further subjected to pfge and investigated for their adhesion abilities in polystyrene microtiter plates. l. monocytogenes was fou ... | 2014 | 24769142 |
| detailed sar and pca of the tyrocidines and analogues towards leucocin a-sensitive and leucocin a-resistant listeria monocytogenes. | the tyrocidines, antimicrobial cyclic decapeptides from bacillus aneurinolyticus, have potent activity with drug/disinfectant potential, specifically against listeria monocytogenes. the tyrocidine activity is dependent on an amphipathic balance. structure-activity relationship (sar) analysis combined with principal component analysis showed the best activity correlation with hydropathy and solvent accessible volume (hydrophobicity parameters), mr and molecular volume (steric/size parameters), co ... | 2014 | 24766734 |
| bruker biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of nocardia, rhodococcus, kocuria, gordonia, tsukamurella, and listeria species. | we evaluated whether the bruker biotyper matrix-associated laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) system provides accurate species-level identifications of 147 isolates of aerobically growing gram-positive rods (gprs). the bacterial isolates included nocardia (n = 74), listeria (n = 39), kocuria (n = 15), rhodococcus (n = 10), gordonia (n = 7), and tsukamurella (n = 2) species, which had all been identified by conventional methods, molecular methods, or both. ... | 2014 | 24759706 |
| self-emulsification of alkaline-dissolved clove bud oil by whey protein, gum arabic, lecithin, and their combinations. | low-cost emulsification technologies using food ingredients are critical to various applications. in the present study, a novel self-emulsification technique was studied to prepare clove bud oil (cbo) emulsions, without specialized equipment or organic solvents. cbo was first dissolved in hot alkaline solutions, added at 1% v/v into neutral solutions with 1% w/v emulsifier composed of whey protein concentrate (wpc), gum arabic, lecithin, or their equal mass mixtures, and adjusted to ph 7.0. the ... | 2014 | 24758517 |
| antimicrobial n-halamine modified polyethylene: characterization, biocidal efficacy, regeneration, and stability. | development of antimicrobial materials that regenerate antimicrobial activity represents a novel technology in preventing microbial cross-contamination. we report a method for the application of regenerably antimicrobial n-halamines onto the surface of polyethylene (pe) materials through layer-by-layer (lbl) assembly of polyethyleneimine and poly(acrylic acid). a total of 5, 10, 15, and 20 bilayers were applied. modified pe had from 49.3 to 293.5 nmol cm(-2) antimicrobial n-halamines from 5 to 2 ... | 2014 | 24754818 |
| technologies and mechanisms for safety control of ready-to-eat muscle foods: an updated review. | ready-to-eat (rte) muscle foods refer to a general category of meat and poultry products that are fully cooked and consumable without reheating. these products, including whole and sliced pork, beef, turkey, chicken, and variety of meats, in the forms of ham, roast, rolls, sausage, and frankfurter, are widely available in the delicatessen section of retail stores or various food service outlets. however, difficulties in avoidance of contamination by foodborne pathogens, notably listeria monocyto ... | 2015 | 24754253 |
| predictive modeling for growth of non- and cold-adapted listeria monocytogenes on fresh-cut cantaloupe at different storage temperatures. | the aim of this study was to determine the growth kinetics of listeria monocytogenes, with and without cold-adaption, on fresh-cut cantaloupe under different storage temperatures. fresh-cut samples, spot inoculated with a 4-strain cocktail of l. monocytogenes (∼3.2 log cfu/g), were exposed to constant storage temperatures held at 10, 15, 20, 25, or 30 °c. all growth curves of l. monocytogenes were fitted to the baranyi, modified gompertz, and huang models. regardless of conditions under which ce ... | 2014 | 24754226 |
| crystal structure of listeriolysin o reveals molecular details of oligomerization and pore formation. | listeriolysin o (llo) is an essential virulence factor of listeria monocytogenes that causes listeriosis. listeria monocytogenes owes its ability to live within cells to the ph- and temperature-dependent pore-forming activity of llo, which is unique among cholesterol-dependent cytolysins. llo enables the bacteria to cross the phagosomal membrane and is also involved in activation of cellular processes, including the modulation of gene expression or intracellular ca(2+) oscillations. neither the ... | 2014 | 24751541 |
| urban prevalence of listeria spp. and listeria monocytogenes in public lavatories and on shoe soles of facility patrons in the european capital city vienna. | the aim of this study was to determine the prevalence of listeria spp. and listeria monocytogenes (l. monocytogenes) in urban public lavatories and on shoe soles of facility patrons in a european capital city. more than 91% of all municipal public lavatories in vienna close to public hubs were included in this study. overall, 373 swab samples of public lavatories and shoes of facility patrons were enriched, according to iso 11290-1. listeria monocytogenes isolates were subtyped using pulsed-fiel ... | 2015 | 24751465 |
| microbiological sampling plan based on risk classification to verify supplier selection and production of served meals in food service operation. | food service operations are confronted with a diverse range of raw materials and served meals. the implementation of a microbial sampling plan in the framework of verification of suppliers and their own production process (functionality of their prerequisite and haccp program), demands selection of food products and sampling frequencies. however, these are often selected without a well described scientifically underpinned sampling plan. therefore, an approach on how to set-up a focused sampling ... | 2014 | 24750814 |
| combined treatments of high-pressure with the lactoperoxidase system or lactoferrin on the inactivation of listeria monocytogenes, salmonella enteritidis and escherichia coli o157:h7 in beef carpaccio. | the effect of high hydrostatic pressure (hhp) treatments in combination with the lactoperoxidase system (lpos) or activated lactoferrin (alf) on listeria monocytogenes, salmonella enterica subsp. enterica serovar enteritidis and escherichia coli o157:h7 was investigated in cured beef carpaccio stored at 8 °c or 22 °c during 7 d. hhp (450 mpa for 5 min) reduced pathogen levels by 1-3 log units and the antimicrobial effect remained during 7 d of storage under temperature abuse conditions at 8 °c a ... | 2014 | 24750810 |
| physical and antibacterial properties of açaí edible films formulated with thyme essential oil and apple skin polyphenols. | thyme essential oil (teo) and apple skin polyphenols (asp) are natural compounds considered as generally recognized as safe by fda, with biological effects against bacteria and fungi. this work aimed to evaluate physical and antimicrobial properties of açaí edible films formulated with teo and asp at 3% and 6% (w/w) individually or combined at 3% (w/w) each. physical properties studied include mechanical resistance, water vapor permeability (wvp), color, and thermal resistance. antimicrobial act ... | 2014 | 24749789 |
| focused specificity of intestinal th17 cells towards commensal bacterial antigens. | t-helper-17 (th17) cells have critical roles in mucosal defence and in autoimmune disease pathogenesis. they are most abundant in the small intestine lamina propria, where their presence requires colonization of mice with microbiota. segmented filamentous bacteria (sfb) are sufficient to induce th17 cells and to promote th17-dependent autoimmune disease in animal models. however, the specificity of th17 cells, the mechanism of their induction by distinct bacteria, and the means by which they fos ... | 2014 | 24739972 |
| listeria monocytogenes exploits efferocytosis to promote cell-to-cell spread. | efferocytosis, the process by which dying or dead cells are removed by phagocytosis, has an important role in development, tissue homeostasis and innate immunity. efferocytosis is mediated, in part, by receptors that bind to exofacial phosphatidylserine (ps) on cells or cellular debris after loss of plasma membrane asymmetry. here we show that a bacterial pathogen, listeria monocytogenes, can exploit efferocytosis to promote cell-to-cell spread during infection. these bacteria can escape the pha ... | 2014 | 24739967 |
| tracing innate immune defences along the path of listeria monocytogenes infection. | the pathogenic gram-positive bacteria, listeria monocytogenes is a facultative infectious intracellular pathogen that causes listeriosis. effective elimination of infection is dependent upon a functioning innate immune system and activation of inflammatory responses by pathogen recognition receptors (prrs). in this review, we trace the route of l. monocytogenes invasion as it disseminates from the intestinal epithelium, through the bloodstream of the host, to the liver and spleen. along this rou ... | 2014 | 24732075 |
| role of pdxr in the activation of vitamin b6 biosynthesis in listeria monocytogenes. | listeria monocytogenes pdxr is a member of the poorly characterized but widespread group of mocr/gabr-type chimeric bacterial proteins that have dna-binding and aminotransferase-like domains. using mutational analysis, real-time rt-pcr, transcriptional fusions, gel-shift assays, dnase i footprinting, and in vitro transcription, it was shown that pdxr is a direct activator of the pdxst operon, transcribed divergently from pdxr and responsible for the de novo synthesis of pyridoxal 5'-phosphate (p ... | 2014 | 24730374 |
| tyk2-dependent bystander activation of conventional and nonconventional th1 cell subsets contributes to innate host defense against listeria monocytogenes infection. | il-12, which is produced in response to intracellular bacteria, such as listeria monocytogenes, promotes the development of pathogen-specific th1 cells that play an important role in host defense. however, it has also been known that cd44(high) memory-phenotype cd4 t cells with th1 functions naturally occur in naive mice, and that lymphopenia-induced proliferation of naive cd4 t cells generates memory-phenotype cd4 t cells with th1 functions, although their differentiation mechanism and contribu ... | 2014 | 24729620 |
| septicaemia models using streptococcus pneumoniae and listeria monocytogenes: understanding the role of complement properdin. | streptococcus pneumoniae and listeria monocytogenes, pathogens which can cause severe infectious disease in human, were used to infect properdin-deficient and wildtype mice. the aim was to deduce a role for properdin, positive regulator of the alternative pathway of complement activation, by comparing and contrasting the immune response of the two genotypes in vivo. we show that properdin-deficient and wildtype mice mounted antipneumococcal serotype-specific igm antibodies, which were protective ... | 2014 | 24728387 |
| tlr9 agonist regulates angiogenesis and inhibits corneal neovascularization. | myeloid cells are highly adaptable and may positively or negatively regulate angiogenesis dependent on the cognate and soluble signals they receive. toll-like receptors (tlrs) initiate immune responses, orchestrate adaptive immune responses, and regulate vascular endothelial growth factor (vegf)-mediated angiogenesis during wound healing. we investigated the possible role of tlr ligands in attenuation of new vessel growth via regulation of expression of vegf or soluble fms-like tyrosine kinase-1 ... | 2014 | 24726642 |
| draft genome sequences of six listeria monocytogenes strains isolated from dairy products from a processing plant in southern italy. | here we announce the draft genome sequences of 6 listeria monocytogenes strains from ricotta cheese produced in a dairy processing plant located in southern italy and potentially involved in a multistate outbreak of listeriosis in the united states. | 2014 | 24723720 |
| ctla-4 (cd152) enhances the tc17 differentiation program. | although cd8(+) t cells that produce il-17 (tc17 cells) have been linked to host defense, tc17 cells show reduced cytotoxic activity, which is the characteristic function of cd8(+) t cells. here, we show that ctla-4 enhances the frequency of il-17 in cd8(+) t cells, indicating that ctla-4 (cd152) specifically promotes tc17 differentiation. simultaneous stimulation of ctla-4(+/+) and ctla-4(-/-) t cells in cocultures and agonistic ctla-4 stimulation unambiguously revealed a cell-intrinsic mechani ... | 2014 | 24723371 |
| antimicrobial susceptibility of listeria monocytogenes isolates from human cases in northern italy, 2008-2010: mic determination according to eucast broth microdilution method. | susceptibility of 96 listeria monocytogenes human isolates collected in northern italy between 2008 and 2010, to 15 antimicrobials, was investigated. minimum inhibitory concentration (mic) was evaluated by means of the standardized broth microdilution method, according to the european committee on antimicrobial susceptibility testing (eucast) international guidelines. all l. monocytogenes human isolates were susceptible to penicillin g (mic(90)≤0·06 μg/ml), meropenem (mic(90)≤0·06 μg/ml), and er ... | 2015 | 24720267 |
| no induction of antimicrobial resistance in staphylococcus aureus and listeria monocytogenes during continuous exposure to eugenol and citral. | the aim of this study was to evaluate the adaptation response of staphylococcus aureus, methicillin-resistant s. aureus (mrsa), and listeria monocytogenes to the essential oil (eo), eugenol, and citral. the minimum inhibitory concentration of eugenol and citral was determined by agar dilution and microdilution. adaptation to eugenol and citral was done by sequential exposure of the pathogens to increasing concentrations of the essential oils. the m2-a9 standard was used to determine the antibiot ... | 2014 | 24716611 |
| stat1β is not dominant negative and is capable of contributing to gamma interferon-dependent innate immunity. | the transcription factor stat1 is essential for interferon (ifn)-mediated immunity in humans and mice. stat1 function is tightly regulated, and both loss- and gain-of-function mutations result in severe immune diseases. the two alternatively spliced isoforms, stat1α and stat1β, differ with regard to a c-terminal transactivation domain, which is absent in stat1β. stat1β is considered to be transcriptionally inactive and to be a competitive inhibitor of stat1α. to investigate the functions of the ... | 2014 | 24710278 |
| myrtus communis essential oil: chemical composition and antimicrobial activities against food spoilage pathogens. | myrtus communis is a typical plant of the mediterranean area, which is mainly used as animal and human food and, in folk medicine, for treating some disorders. in the present study, we evaluated in vitro antibacterial and antifungal properties of the essential oils of myrtus communis (mceo), as well as its phytochemical composition. the gc/ms analysis of the essential oil revealed 17 compounds. myrtenyl acetate (20.75%), 1,8-cineol (16.55%), α-pinene (15.59%), linalool (13.30%), limonene (8.94%) ... | 2014 | 24706627 |
| population structure of listeria monocytogenes serotype 4b isolates from sporadic human listeriosis cases in the united states from 2003 to 2008. | listeria monocytogenes can cause severe food-borne disease (listeriosis). numerous outbreaks have involved three serotype 4b epidemic clones (ecs): eci, ecii, and ecia. however, little is known about the population structure of l. monocytogenes serotype 4b from sporadic listeriosis in the united states, even though most cases of human listeriosis are in fact sporadic. here we analyzed 136 serotype 4b isolates from sporadic cases in the united states, 2003 to 2008, utilizing multiple tools includ ... | 2014 | 24705322 |
| long-term exposure to decabrominated diphenyl ether impairs cd8 t-cell function in adult mice. | polybrominated diphenyl ethers (pbdes) are ubiquitous environmental pollutants that accumulate to high levels in human populations that are subject to occupational or regional industry exposure. pbdes have been shown to affect human neuronal, endocrine and reproductive systems, but their effect on the immune system is not well understood. in this study, experimental adult mice were intragastrically administered 2,2',3,3',4,4',5,5',6,6'-decabromodiphenyl ether (bde-209) at doses of 8, 80 or 800 m ... | 2014 | 24705197 |
| charge counter charge: bacterial response to antimicrobial cationic peptides and more. | | 2014 | 24705072 |
| suppression of osteoblast toll-like receptor 2 signaling by endothelin-1. | peripheral endothelin-1 (et-1) levels are increased in chronic systemic disorders such as congestive cardiac failure, diabetes and chronic renal failure. bone infections are also associated with poor prognoses in these conditions. in the present study, we examined the alterations in toll-like receptor 2 (tlr2) signaling induced by et-1 in an in vitro osteoblast cell model. the tlr2-positive murine osteoblast cell line mc3t3-e1 was treated with heat-killed listeria monocytogenes (hklm), a tlr2 li ... | 2014 | 24700498 |
| notes from the field: multistate outbreak of listeriosis linked to soft-ripened cheese--united states, 2013. | on june 27, 2013, the minnesota department of health notified cdc of two patients with invasive listeria monocytogenes infections (listeriosis) whose clinical isolates had indistinguishable pulsed-field gel electrophoresis (pfge) patterns. a query of pulsenet, the national molecular subtyping network for foodborne disease surveillance, identified clinical and environmental isolates from other states. on june 28, cdc learned from the food and drug administration's coordinated outbreak response an ... | 2014 | 24699767 |
| optimization of combinations of bactericidal and bacteriostatic treatments to control listeria monocytogenes on cold-smoked salmon. | contamination of cold-smoked salmon by listeria monocytogenes is a major concern for the seafood industry. the objectives of this study were to (i) determine the most effective bactericidal treatment for l. monocytogenes on salmon and (ii) optimize bactericidal and bacteriostatic treatment combinations to identify cost-effective treatments against l. monocytogenes on salmon. l. monocytogenes challenge trials were conducted in brain heart infusion (bhi) and on salmon disks that were supplemented ... | 2014 | 24699233 |
| whole genome sequencing as a tool to investigate a cluster of seven cases of listeriosis in austria and germany, 2011-2013. | a cluster of seven human cases of listeriosis occurred in austria and in germany between april 2011 and july 2013. the listeria monocytogenes serovar (sv) 1/2b isolates shared pulsed-field gel electrophoresis (pfge) and fluorescent amplified fragment length polymorphism (faflp) patterns indistinguishable from those from five food producers. the seven human isolates, a control strain with a different pfge/faflp profile and ten food isolates were subjected to whole genome sequencing (wgs) in a bli ... | 2014 | 24698214 |
| listeria: an australian perspective (2001-2010). | despite having a low occurrence rate, listeria monocytogenes is one of the most prominent foodborne pathogens in australia. the organism is responsible for severe outbreaks with high case fatality and substantial economic losses due to food recalls. in this study, we analyze the incidence trends of listeriosis in australia during 2001-2010, discuss the relevance of food recalls, and investigate the pathogen's role in foodborne outbreaks. a significant epidemiological finding was a consistently h ... | 2014 | 24697613 |
| [immunobiologic characteristics of a recombinant listeria monocytogenes expressing mycobacterium tuberculosis antigens]. | tuberculosis is a chronic infectious disease caused by mycobacterium tuberculosis complex. hence, novel vaccines against tb are urgently needed and important to the public health. | 2013 | 24697107 |
| liofilchem(®) o.a. listeria agar and direct camp test provided sooner listeria monocytogenes identification from neonatal bacteremia. | listeria monocytogenes infection in pregnant women and newborns is a cause for serious concern, and invasive disease outcome strongly depends on prompt antibiotic therapy. to provide sooner identification from neonatal bacteremia we performed a camp test directly on positive blood aliquots and inoculated the liofilchem(®) o.a. listeria chromogenic agar as well, thus providing a 24-h turn-around time for response. | 2014 | 24695762 |
| multi-virulence-locus sequence typing of 4b listeria monocytogenes isolates obtained from different sources in india over a 10-year period. | listeria monocytogenes is an emerging foodborne pathogen responsible for listeriosis. the incidence of listeriosis has increased during the last 2 decades due to the increase in consumption of ready-to-eat foods and change in food consumption habits. outbreaks and sporadic cases of listeriosis have been reported in developed countries. these reports have helped determine the safety practices needed to control listeriosis. although l. monocytogenes has been reported from humans, animals, and a va ... | 2014 | 24694111 |
| essential oils of thyme and rosemary in the control of listeria monocytogenes in raw beef. | this study was developed in order to evaluate two alternatives for the control of listeria monocytogenes in raw bovine meat pieces, both based on the use of thymus vulgaris and rosmarinus officinalis essential oils (eos). the antilisterial activity of different concentrations of the eos was tested in vitro using agar dilution and disk volatilization techniques. in addition, l. monocytogenes was inoculated in meat pieces, which were submerged in edible gelatin coatings containing 2% (v/v) eos or ... | 2013 | 24688509 |
| prevalence and quantification of listeria monocytogenes in beef offal at retail level in selangor, malaysia. | a total of 63 beef offal samples (beef liver = 16; beef lung = 14; beef intestine = 9; beef tripe = 15; beef spleen = 9) from three wet markets (a, b, and c) in selangor, malaysia were examined for the prevalence and microbial load of listeria monocytogenes. a combination of the most probable number and polymerase chain reaction (mpn-pcr) method was employed in this study. it was found that l. monocytogenes detected in 33.33% of the beef offal samples. the prevalence of l. monocytogenes in beef ... | 2013 | 24688507 |
| antibacterial activity of bacteriocin-like substance p34 on listeria monocytogenes in chicken sausage. | the antimicrobial activity of the bacteriocin-like substance (bls) p34 against listeria monocytogenes was investigated in chicken sausage. the bls was applied to chicken sausages (256 au g(-1)) previously inoculated with a suspension of 10(2) cfu g(-1) of l. monocytogenes. bls p34 inhibited the indicator microorganism in situ in all incubation times for up to 10 days at 5 °c. the effectiveness of bls p34 was increased when it was added in combination with nisin. the bacteriocin was also tested i ... | 2013 | 24688506 |
| heat shock protein vaccination and directed il-2 therapy amplify tumor immunity rapidly following bone marrow transplantation in mice. | tumor relapse is the primary cause of mortality in patients with hematologic cancers following autologous hematopoietic stem cell transplantation (hsct). vaccination early after hsct can exploit both the state of lymphopenia and minimal residual disease for generating antitumor immunity. here, multiple vaccinations using lymphoma cells engineered to secrete heat shock protein fusion gp96-ig within 2 weeks of t cell-replete syngeneic hsct led to cross-presentation and increased survival of lympho ... | 2014 | 24687086 |
| [spontaneous listeria monocytogenes bacterial peritonitis]. | | 2014 | 24684887 |
| cell depletion in mice that express diphtheria toxin receptor under the control of siglech encompasses more than plasmacytoid dendritic cells. | plasmacytoid dendritic cells (pdc) produce ifn-i in response to viruses and are routinely identified in mice by siglech expression. siglech is a sialic acid-binding ig-like lectin that has an immunomodulatory role during viral infections. in this study, we evaluated the impact of siglech deficiency on cytokine responses in the presence and absence of pdc. we found that lack of siglech enhanced ifn-i responses to viral infection, regardless of whether pdc were depleted. we also examined the expre ... | 2014 | 24683186 |
| reducing time in the analysis of listeria monocytogenes in meat, dairy and vegetable products. | the microbiological standard for detection of listeria monocytogenes relies on several cultural steps and requires 7 days for final confirmation, and due to food distribution and market demands, there is a prevailing need for an alternative methodology for its detection. the aim of this study was to compare different detection strategies based on real-time pcr (rti-pcr) for a rapid and sensitive detection in an ample range of food products: raw pork and poultry meat, raw sheep milk cured cheese, ... | 2014 | 24679921 |
| behavioral risk factors associated with listeriosis in the home: a review of consumer food safety studies. | listeria monocytogenes causes human listeriosis, which is associated with the highest hospitalization and mortality rates of all foodborne illnesses. in recent years, the incidence of listeriosis has doubled in europe, almost exclusively among older adults (≥ 60 years of age). food safety factors associated with increased risk of listeriosis include lack of adherence to "use by" dates and ineffective refrigerated storage of foods. consequently, older adult consumers' implementation of safe food ... | 2014 | 24674447 |
| rapid detection of listeria monocytogenes by real-time pcr in processed meat and dairy products. | the objectives of this study were to evaluate the detection of listeria monocytogenes in different ready-to-eat foods using real-time pcr (rt-pcr). various concentrations (10(0) to 10(5) cfu/ml) of l. monocytogenes atcc 19115 were inoculated into ham, sausage, ground meat, processed milk, cheese, and infant formula. l. monocytogenes atcc 19115 in the samples was then enumerated on oxford agar, and dna was extracted from the samples before and after incubation at 36°c for 4 h. a set of primers an ... | 2014 | 24674437 |
| characterization of mexican coriander (eryngium foetidum) essential oil and its inactivation of listeria monocytogenes in vitro and during mild thermal pasteurization of pineapple juice. | the aim of this work was to characterize the essential oil (eo) of eryngium foetidum (efeo) and assess its activity toward listeria monocytogenes in broth and during thermal inactivation of the pathogen in pineapple juice. in this respect, efeo was chemically characterized, and its antilisteria potential in broth as a function of ph, cell load, and efeo concentration was assessed through a central composite design. furthermore, the inactivation kinetics of l. monocytogenes in the juice were asse ... | 2014 | 24674435 |
| evaluation of the thermo scientific suretect listeria monocytogenes assay. | the thermo scientific suretect listeria monocytogenes assay is a new real-time pcr assay for the detection of listeria monocytogenes in food and environmental samples. this assay was validated using the aoac research institute (aoac-ri) performance tested methods program in comparison to the reference method detailed in international organization for standardization 11290-1:1996, including amendment 1:2004 with the following foods and food contact surfaces: smoked salmon, processed cheese, fresh ... | 2014 | 24672870 |
| crystal structure of an engineered yopm-inlb hybrid protein. | the multi-domain protein inlb (internalin b) from listeria monocytogenes is an agonist of the human receptor tyrosine kinase met. only the internalin domain directly interacts with met. the internalin domain consists of seven central leucine-rich repeats (lrrs) flanked by an n-terminal helical cap domain and a c-terminal immunoglobulin-like structure. a potential function of the n-terminal cap in receptor binding could so far not be demonstrated by deleting the cap, since the cap is also implica ... | 2014 | 24669959 |
| pigmented hypopyon in association with listeria monocytogenes endopthalmitis: an interesting case report following refractive surgery procedure with literature review. | listeria monocytogenes is an aerobic, motile, gram positive bacillus recognized as an intercellular pathogen in human where it most frequently affects neonates, pregnant women, elderly patients, and immunosuppressed individuals as well as healthy persons. ocular listeriosis is rare, most frequently in the form of conjunctivitis, but has been also shown to cause rarely endophthalmitis with pigmented hypopyon and elevated intraocular pressure such as in our case. | 2017 | 24669144 |
| comparison of widely used listeria monocytogenes strains egd, 10403s, and egd-e highlights genomic variations underlying differences in pathogenicity. | for nearly 3 decades, listeriologists and immunologists have used mainly three strains of the same serovar (1/2a) to analyze the virulence of the bacterial pathogen listeria monocytogenes. the genomes of two of these strains, egd-e and 10403s, were released in 2001 and 2008, respectively. here we report the genome sequence of the third reference strain, egd, and extensive genomic and phenotypic comparisons of the three strains. strikingly, egd-e is genetically highly distinct from egd (29,016 si ... | 2014 | 24667708 |
| antibiotic susceptibility in benzalkonium chloride-resistant and -susceptible listeria monocytogenes strains. | this study aimed to investigate whether listeria monocytogenes strains with resistance to a commonly used biocide display any cross-resistance to antibiotics. using pulsed-field gel electrophoresis (pfge), 29 different pfge types were previously identified in an iberian pig abattoir and processing plant. only three pfge types were resistant to benzalkonium chloride (bac), but they represented a significant proportion of the pfge types surviving in the plant after 4 years. in the present study, a ... | 2014 | 24666359 |
| probabilistic approach for determining salmonella spp. and l. monocytogenes concentration in pork meat from presence/absence microbiological data. | in the present study, prevalence and concentration of salmonella spp. and listeria monocytogenes in fresh pork cuts were determined through the analysis of twelve lots for one year. five samples were analyzed at retail and after storage at 4 and 12°c up to the end of shelf-life (7 days). results obtained for salmonella spp. indicated that a total of 15 samples (8.33%) were positive, which represents 4 (25%) sampling events positive (i.e. at least one sample was positive in at least one of the sa ... | 2014 | 24661505 |
| [pneumococcal meningitis in a pregnant woman]. | bacterial meningitis is a life-threatening disease. the incidence of meningitis is about 2.6-6 cases per 100.000 adults per year in developed countries. the most common causative microorganisms are sreptococcus pneumoniae and neisseria meningitidis. a 33-year-old multigravida, at 24 week of gestation was admitted to the hospital because of ear pain, haedache, fever and confusion. lumbal puncture was performed and cerebrospinal fluid analysis showed signs of bacterial meningitis. latex agglutinat ... | 2014 | 24654448 |
| [brainstem encephalitis and myelitis due to listeria monocytogenes: a case report and literature review]. | brainstem encephalitis caused by listeria monocytogenes is an uncommon form of central nervous system listeriosis; however, it is the most common presentation in immunocompetent individuals. here, we describe an even more rare combination of rhombencephalitis with severe myelitis caused by l. monocytogenes in an immunocompetent patient. we report the case of a 21-year-old immunocompetent patient who consumed unpasteurized dairy products and experienced headache and vomiting that progressed to an ... | 2014 | 24652169 |
| [listeria monocytogenes in food handlers: a new approach to address the dangers in food industry]. | foodborne diseases are a high-impact health problem. listeria monocytogenes is frequently associated with contaminated meat and dairy foods. monitoring their presence at various stages during production is important to control the spread of this microorganism. | 2014 | 24652139 |
| comparison of subtypes of listeria monocytogenes isolates from naturally contaminated watershed samples with and without a selective secondary enrichment. | two enrichment methods for listeria monocytogenes using immuno magnetic separation (ims) were tested to determine if they selected the same subtypes of isolates. both methods used a non-selective primary enrichment and one included subculture in fraser broth, while the other involved direct plating of ims beads. sixty-two naturally contaminated watershed samples from the central california coast were used as a source of l. monocytogenes, and subtype diversity was measured by serotype and multipl ... | 2014 | 24651315 |