| modelling the effect of lactic acid bacteria from starter- and aroma culture on growth of listeria monocytogenes in cottage cheese. | four mathematical models were developed and validated for simultaneous growth of mesophilic lactic acid bacteria from added cultures and listeria monocytogenes, during chilled storage of cottage cheese with fresh- or cultured cream dressing. the mathematical models include the effect of temperature, ph, nacl, lactic- and sorbic acid and the interaction between these environmental factors. growth models were developed by combining new and existing cardinal parameter values. subsequently, the refe ... | 2014 | 25086348 |
| diversity and distribution of listeria monocytogenes in meat processing plants. | listeria monocytogenes is a major concern for the meat processing industry because many listeriosis outbreaks have been linked to meat product consumption. the aim of this study was to elucidate l. monocytogenes diversity and distribution across different spanish meat processing plants. l. monocytogenes isolates (n = 106) collected from food contact surfaces of meat processing plants and meat products were serotyped and then characterised by multilocus sequence typing (mlst). the isolates were s ... | 2014 | 25084653 |
| efficacy of plant-derived compounds combined with hydrogen peroxide as antimicrobial wash and coating treatment for reducing listeria monocytogenes on cantaloupes. | the efficacy of four plant-derived antimicrobials (pdas), namely carvacrol, thymol, β-resorcylic acid, and caprylic acid, with or without hydrogen peroxide (hp), as antimicrobial wash and chitosan based coating for reducing listeria monocytogenes (lm) on cantaloupes was investigated. cantaloupe rind plugs inoculated with lm (10(7) cfu/cm(2)) were washed for 3, 6, 10 min at 25 °c or 1, 3, 5 min at 55 or 65 °c in water, or water containing 2% pdas with or without 2% hp. additionally, inoculated ca ... | 2014 | 25084644 |
| radiation therapy combined with listeria monocytogenes-based cancer vaccine synergize to enhance tumor control in the b16 melanoma model. | conceptually, the immune system may profoundly influence the efficacy of radiation therapy. compelling evidence has recently emerged revealing the capacity of local radiation therapy (rt) to induce antitumor immune responses and sparked interest in combining rt with immunotherapy to promote tumor-specific immunity. a listeria monocytogenes (lm)-based cancer vaccine engineered to express tumor-associated antigen has been shown to effectively retard tumor growth by cell-mediated immune mechanisms. ... | 2014 | 25083327 |
| attenuated listeria monocytogenes reprograms m2-polarized tumor-associated macrophages in ovarian cancer leading to inos-mediated tumor cell lysis. | a principal mechanism by which tumors evade immune-mediated elimination is through immunosuppression. previous approaches to tumor immunotherapy have focused on modifying the immunosuppressive environment with immune checkpoint inhibitors, cytokine therapy, and other modalities with the intent to generate t-cell based anti-tumor immunity. we hypothesized that transformation of the suppressive ovarian cancer microenvironment could be achieved by introduction of the attenuated δacta/δinlb strain o ... | 2014 | 25083323 |
| the rab5-gef function of rin1 regulates multiple steps during listeria monocytogenes infection. | listeria monocytogenes is a food-borne pathogenic bacterium that invades intestinal epithelial cells through a phagocytic pathway that relies on the activation of host cell rab5 gtpases. listeria monocytogenes must subsequently inhibit rab5, however, in order to escape lysosome-mediated destruction. relatively little is known about upstream rab5 regulators during l. monocytogenes entry and phagosome escape processes in epithelial cells. here we identify rin1, a ras effector and rab5-directed gua ... | 2014 | 25082076 |
| stability, compatibility and microbiological activity studies of meropenem-clavulanate potassium. | meropenem (mem) and clavulanate potassium have been reported to demonstrate highly effective activity against mycobacterium tuberculosis. there have been no reports on research into the complex of these chemotherapeutics concerning their mutually dependent stability or microbiological action on other microorganisms. stability and compatibility studies of mem/clavulanate potassium were conducted by using an hplc-dad method. the antibacterial activity of mem/clavulanate potassium was tested in vit ... | 2015 | 25074659 |
| modeling the growth of listeria monocytogenes on the surface of smear- or mold-ripened cheese. | surface-ripened cheeses are matured by means of manual or mechanical technologies posing a risk of cross-contamination, if any cheeses are contaminated with listeria monocytogenes. in predictive microbiology, primary models are used to describe microbial responses, such as growth rate over time and secondary models explain how those responses change with environmental factors. in this way, primary models were used to assess the growth rate of l. monocytogenes during ripening of the cheeses and t ... | 2014 | 25072033 |
| systemic listeria monocytogenes infection in a 2-year-old immunocompetent child. | central nervous system infection and sepsis are the most frequently observed clinical presentations of listeriosis infection; however, they are rare in immunocompetent children beyond the neonatal period. in the presented case, we described gastrointestinal involvement, subacute meningitis, sinusitis and sepsis in a two-year-old previously healthy child with acute infection caused by listeria monocytogenes. we suggest that the infection was probably enhanced by an inappropriate corticosteroid tr ... | 2014 | 25060681 |
| draft genome sequence of listeria monocytogenes strain li0521 (syn. hpb7171), isolated in 1983 during an outbreak in massachusetts caused by contaminated cheese. | listeria monocytogenes, a pathogenic food-borne bacterium, is the causative agent of both sporadic and outbreak cases of human listeriosis. here, we present the genome sequence of l. monocytogenes reference strain li0521, isolated during an outbreak involving contaminated cheese, which has been used as the model during several proteomic studies. | 2014 | 25059873 |
| listeria meningitis and resultant symptomatic hydrocephalus complicating infliximab treatment for ulcerative colitis. | listeria monocytogenes, an uncommon pathogen, should be considered by clinicians as a source of sepsis and meningitis in the immunocompromised individuals, including those on anti-tnf alpha agents. immunosuppressant agents including biologic therapies have transformed the management of various rheumatological and dermatological conditions.(1) we report a case of life-threatening l. monocytogenes sepsis and meningitis in a 75-year-old man receiving infliximab for severe ulcerative colitis (uc). | 2014 | 25057381 |
| genetic classification of listeria monocytogenes serotype 4b strains, including epidemic clones, isolated from retail meat in the tokyo metropolitan area. | a food-borne pathogen, listeria monocytogenes serotype 4b, has been frequently isolated from patients with listeriosis, and numerous outbreaks of listeriosis are associated with this serotype. in the present study, we performed subtyping of l. monocytogenes serotype 4b strains on the basis of genetic analyses. thirty-four isolates of serotype 4b were classified into 8 genotypes, namely genotypes 12, 15, 16, 17, 18, 23, 24, and 25, on the basis of the sequence for the partial iap gene. genetic an ... | 2014 | 25056070 |
| instantlabs listeria monocytogenes food safety kit. performance tested method 051304. | the instantlabs listeria monocytogenes food safety kit was validated against the international organization for standardization (iso) reference method 11290-1 for the detection of listeria monocytogenes and other listeria species. the matrixes (stainless steel, sealed concrete, ice cream, whole milk, cheddar cheese, raw shrimp, hot dogs, deli turkey, and lettuce) were inoculated with approximately 1 cfu/test portion of l. monocytogenes to generate fractional positives (5-15) in 20 inoculated sam ... | 2014 | 25051634 |
| instantlabs listeria species food safety kit. performance tested methods 041304. | the instantlabs listeria species food safetykitwas validated against the international organization for standardization (iso) reference method 11290-1 for the detection of listeria monocytogenes and other listeria species. the matrixes (stainless steel, sealed concrete, cheddar cheese, raw shrimp, and hot dogs) were inoculated with approximately 1 cfu/test portion of various listeria species to generate fractional positives (5-15) in 20 inoculated samples. enrichments were also fractionally inoc ... | 2014 | 25051633 |
| deletion of the membrane protein lmo0412 increases the virulence of listeria monocytogenes. | listeria monocytogenes is a facultative intracellular pathogen that causes gastroenteritis, meningitis, encephalitis and maternofetal infections. 20-30% of eubacterial orfs are predicted to encode membrane proteins. the bacterial cytoplasmic membrane is a macromolecular structure, which plays a key role for the pathogenesis. despite this, little knowledge exists regarding the function of cytoplasmic membrane proteins of listeria during infection. here, we investigated a predicted membrane protei ... | 2014 | 25051038 |
| assessment of oligogalacturonide from citrus pectin as a potential antibacterial agent against foodborne pathogens. | foodborne diseases are an important public health problem in the world. the bacterial resistance against presently used antibiotics is becoming a public health issue; hence, the discovery of new antimicrobial agents from natural sources attracts a lot of attention. antibacterial activities of oligogalacturonide from commercial microbial pectic enzyme (cpe) treated citrus pectin, which exhibits antioxidant and antitumor activities, against 4 foodborne pathogens including salmonella typhimurium, s ... | 2014 | 25048440 |
| serogrouping and pulsed-field gel electrophoresis analysis of listeria monocytogenes isolates from cases of human infection in hungary 2004–2012 molecular typing of listeria monocytogenes in hungary. | eighty isolates of listeria monocytogenes cultured from human infections in hungary between 2004 and 2012 were serotyped by the pcr technique of doumithet al. [9] and characterised by pulsed-field gel electrophoresis (pfge). most of the isolates belonged to two serogroups: 53 isolates (66.3%) to serovar group 4b,4d,4e and 21isolates (25.8%) to serogroup 1/2a,3a. although many pulsotypes were identified a particular pulsotype proved highly excelling comprising of 31 isolates after digestion by bo ... | 2014 | 25046881 |
| dnase i and proteinase k impair listeria monocytogenes biofilm formation and induce dispersal of pre-existing biofilms. | current sanitation methods in the food industry are not always sufficient for prevention or dispersal of listeria monocytogenes biofilms. here, we determined if prevention of adherence or dispersal of existing biofilms could occur if biofilm matrix components were disrupted enzymatically. addition of dnase during biofilm formation reduced attachment (<50% of control) to polystyrene. treatment of established 72h biofilms with 100μg/ml of dnase for 24h induced incomplete biofilm dispersal, with <2 ... | 2014 | 25043896 |
| use of mild irradiation doses to control pathogenic bacteria on meat trimmings for production of patties aiming at provoking minimal changes in quality attributes. | the objectives of the present work were to assess the use of moderate doses of gamma irradiation (2 to 5 kgy) and to reduce the risk of pathogen presence without altering the quality attributes of bovine trimmings and of patties made of irradiated trimmings. microbiological indicators (coliforms, pseudomonas spp and mesophilic aerobic counts), physicochemical indicators (ph, color and tiobarbituric acid) and sensory changes were evaluated during storage. 5 kgy irradiation doses slightly increase ... | 2014 | 25042241 |
| inhibitory effects of the κ-casein macropeptide isolated from milk protein on the biofilm formation and virulence of listeria monocytogenes. | we demonstrate the inhibitory effects of κ-casein macropeptide (cmp) on the biofilm formation and virulence of listeria monocytogenes scott a. the inhibition of biofilm formation by cmp was initially investigated by using the protocol applied for the 96-well microtiter plate assay. low concentrations of cmp (0.1, 0.2, 0.3, 0.4, and 0.5 mg/ml) that were tested resulted in a profound inhibitory effect on biofilm formation at a concentration of 0.4 mg/ml. cmp also significantly repressed the transc ... | 2014 | 25036840 |
| serial transfer of single-cell-derived immunocompetence reveals stemness of cd8(+) central memory t cells. | maintenance of immunological memory has been proposed to rely on stem-cell-like lymphocytes. however, data supporting this hypothesis are focused on the developmental potential of lymphocyte populations and are thus insufficient to establish the functional hallmarks of stemness. here, we investigated self-renewal capacity and multipotency of individual memory lymphocytes by in vivo fate mapping of cd8(+) t cells and their descendants across three generations of serial single-cell adoptive transf ... | 2014 | 25035956 |
| the effect of oleanolic and ursolic acids on the hemolytic properties and biofilm formation of listeria monocytogenes. | oleanolic acid and ursolic acid are pentacyclic triterpenoids isolated from a variety of medicinal plants, which have antibacterial activity. listeria monocytogenes is a gram-positive facultative pathogen, being the causative agent of listeriosis. the present study was carried out to evaluate the in vitro effect of sub-inhibitory concentrations of both triterpene acids on the pathogenicity determinants of l. monocytogenes: their hemolytic activity and biofilm forming ability. oleanolic and ursol ... | 2014 | 25033658 |
| definition of a standard protocol to determine the growth potential of listeria monotgenes and yersinia enterocolitica in pork sausage produced in abruzzo region, italy. | pork meat products consumed raw or after a short period of fermentation can be considered at risk for food safety. sausages (fresh sausage made from pork meat) are produced in several italian regions, with variation in ingredients. in some italian regions, including abruzzo, these products are frequently consumed raw or undercooked, after a variable period of fermentation. the european community food regulation promotes the use of challenge tests to determine safety levels. this study is aimed t ... | 2015 | 27800415 |
| bi-phasic growth of listeria monocytogenes in chemically defined medium at low temperatures. | the present work reports a novel observation regarding the growth of l. monocytogenes in modified welshimer's broth (mwb) at low temperatures. specifically, the direct monitoring of the growth of l. monocytogenes scott a using plate count data revealed that the pathogen displays a bi-phasic growth pattern in mwb at 7 °c. this bi-phasic growth pattern is masked (not observed) when optical density (od) measurements are used to monitor growth due to the inability of od readings to detect l. monocyt ... | 2014 | 25016210 |
| analysing and modelling the growth behaviour of listeria monocytogenes on rte cooked meat products after a high pressure treatment at 400 mpa. | various predictive models are available for high pressure inactivation of listeria monocytogenes in food, but currently available models do not consider the growth kinetics of surviving cells during the subsequent storage of products. therefore, we characterised the growth of l. monocytogenes in sliced cooked meat products after a pressurization treatment. two inoculum levels (10(7) or 10(4) cfu/g) and two physiological states before pressurization (freeze-stressed or cold-adapted) were evaluate ... | 2014 | 25016207 |
| assessment of salmonella and listeria monocytogenes level in ready-to-cook poultry meat: effect of various high pressure treatments and potassium lactate concentrations. | the objective of this study was to develop a probabilistic model in order to determine the contamination level of salmonella and listeria monocytogenes in ready-to-cook poultry meat, after a high pressure (hp) treatment. the model included four steps: i) reception of raw meat materials, mincing and mixing meat, ii) partitioning and packaging into 200-g modified atmosphere packs, iii) high pressure treatment of the meat, and iv) storage in chilled conditions until the end of the shelf-life. the m ... | 2014 | 25016206 |
| purification and mass spectrometry based characterization of a pediocin produced by pediococcus acidilactici 13. | bacteriocins are antimicrobial peptides produced by several bacterial species. among the bacteriocins pediocin-like bacteriocins have a significant inhibitory activity on the foodborne pathogens especially on listeria monocytogenes. this study aims to select a simple and usable purification method to purify/concentrate the antimicrobial peptide and characterization of the bacteriocin produced by pediococcus acidilactici 13 by using proteomic approaches which is a recent omic technology. for puri ... | 2014 | 25015266 |
| a btla-mediated bait and switch strategy permits listeria expansion in cd8α(+) dcs to promote long-term t cell responses. | listeria monocytogenes infected cd8α(+) dcs in the spleen are essential for cd8(+) t cell generation. cd8α(+) dcs are also necessary for listeria expansion and dissemination within the host. the mechanisms that regulate cd8α(+) dcs to allow listeria expansion are unclear. we find that activating the b and t lymphocyte attenuator (btla), a coinhibitory receptor for t cells, suppresses, while blocking btla enhances, both the primary and memory cd8 t cell responses against listeria. btla(-/-) mice ... | 2014 | 25011109 |
| characterization of listeria monocytogenes strains isolated during 2011-2013 from human infections in switzerland. | listeria monocytogenes, an emerging foodborne pathogen, can cause in the population at risk severe infections that are associated with high case fatality rates. a total of 93 l. monocytogenes strains isolated from different patients in switzerland from july 2011 to september 2013 were further characterized. septicemia was reported for 74.2% of the patients, meningitis for 10.8%, and abortion for 3.2%. the majority of the strains belonged to serotype 1/2a (n=58) followed by serotype 4b (n=28), 1/ ... | 2014 | 25007293 |
| reservoirs of listeria species in three environmental ecosystems. | soil and water are suggested to represent pivotal niches for the transmission of listeria monocytogenes to plant material, animals, and the food chain. in the present study, 467 soil and 68 water samples were collected in 12 distinct geological and ecological sites in austria from 2007 to 2009. listeria was present in 30% and 26% of the investigated soil and water samples, respectively. generally, the most dominant species in soil and water samples were listeria seeligeri, l. innocua, and l. iva ... | 2014 | 25002422 |
| response to comment on "tim-3 directly enhances cd8 t cell responses to acute listeria monocytogenes infection". | | 2014 | 24994908 |
| comment on "tim-3 directly enhances cd8 t cell responses to acute listeria monocytogenes infection". | | 2014 | 24994907 |
| perinatal immunization with vaccine-grade listeria monocytogenes provides protection against murine th2 airway inflammation. | asthma is a chronic respiratory disorder that leads to inflammation and narrowing of the airways. its global prevalence has attained epidemic levels and treatment options that reach beyond temporary relief of symptoms are urgently needed. since the processes leading to clinically symptomatic asthma start early in life, we set out to systematically evaluate a neonatal immunotherapeutic based on listeria monocytogenes (lm) for the control of allergic sensitization. | 2014 | 24991458 |
| schistosoma mansoni soluble egg antigens enhance listeria monocytogenes vector hiv-1 vaccine induction of cytotoxic t cells. | vaccines are an important public health measure for prevention and treatment of diseases. in addition to the vaccine immunogen, many vaccines incorporate adjuvants to stimulate the recipient's immune system and enhance vaccine-specific responses. while vaccine development has advanced from attenuated organism to recombinant protein or use of plasmid dna, the development of new adjuvants that safely increase immune responses has not kept pace. previous studies have shown that the complex mixture ... | 2014 | 24990901 |
| inactivation kinetics of escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes in ready-to-eat sliced ham by near-infrared heating at different radiation intensities. | the aim of this study was to investigate the inactivation kinetics of salmonella enterica serovar typhimurium, escherichia coli o157:h7, and listeria monocytogenes on ready-to-eat sliced ham by near-infrared (nir) heating as a function of the processing parameter, radiation intensity. precooked ham slices inoculated with the three pathogens were treated at different nir intensities (ca. 100, 150, and 200 μw/cm(2)/nm). an increase in the applied radiation intensity resulted in a gradual increase ... | 2014 | 24988034 |
| metal-chelating active packaging film enhances lysozyme inhibition of listeria monocytogenes. | several studies have demonstrated that metal chelators enhance the antimicrobial activity of lysozyme. this study examined the effect of metal-chelating active packaging film on the antimicrobial activity of lysozyme against listeria monocytogenes. polypropylene films were surface modified by photoinitiated graft polymerization of acrylic acid (pp-g-paa) from the food contact surface of the films to impart chelating activity based on electrostatic interactions. pp-g-paa exhibited a carboxylic ac ... | 2014 | 24988022 |
| low occurrence of listeria monocytogenes on bovine hides and carcasses in minas gerais state, brazil: molecular characterization and antimicrobial resistance. | listeria monocytogenes occurrence was assessed in three slaughterhouses located in minas gerais state, brazil, by analysis of 209 bovine carcasses. four sponge samples were obtained from each carcass in different steps (a, from hide, before bleeding; b, after hide removal; c, after evisceration; and d, after end washing), resulting in a total of 836 samples. the samples were tested for the presence of l. monocytogenes according to the international organization for standardization 11290-1, and p ... | 2014 | 24988021 |
| the receptor for the complement c3a anaphylatoxin (c3ar) provides host protection against listeria monocytogenes-induced apoptosis. | listeria monocytogenes is a gram-positive intracellular bacterium that is acquired through tainted food and may lead to systemic infection and possible death. despite the importance of the innate immune system in fighting l. monocytogenes infection, little is known about the role of complement and its activation products, including the potent c3a anaphylatoxin. in a model of systemic l. monocytogenes infection, we show that mice lacking the receptor for c3a (c3ar(-/-)) are significantly more sen ... | 2014 | 24981453 |
| listeria monocytogenes dose response revisited--incorporating adjustments for variability in strain virulence and host susceptibility. | evaluations of listeria monocytogenes dose-response relationships are crucially important for risk assessment and risk management, but are complicated by considerable variability across population subgroups and l. monocytogenes strains. despite difficulties associated with the collection of adequate data from outbreak investigations or sporadic cases, the limitations of currently available animal models, and the inability to conduct human volunteer studies, some of the available data now allow r ... | 2015 | 24975545 |
| the surface protein lmo1941 with lysm domain influences cell wall structure and susceptibility of listeria monocytogenes to cephalosporins. | listeria monocytogenes is a gram-positive bacterium causing rare but dangerous cases of disease in humans and animals. the β-lactams penicillin g and ampicillin are the antibiotics of choice in the treatment of listeriosis. recently, lmo1941, encoding a surface protein of l. monocytogenes with unknown function, was identified as a gene transcriptionally upregulated under penicillin g pressure. in this study, the effect of lmo1941 knockout on the susceptibility of l. monocytogenes to β-lactams wa ... | 2014 | 24974853 |
| visual and sensitive detection of viable pathogenic bacteria by sensing of rna markers in gold nanoparticles based paper platform. | food-borne pathogens have been recognized as a major cause of human infections worldwide. their identification needs to be simpler, cheaper and more reliable than the traditional methods. here, we constructed a low-cost paper platform for viable pathogenic bacteria detection with the naked eye. in this study, an effective isothermal amplification method was used to amplify the hlya mrna gene, a specific rna marker in listeria monocytogenes. the amplification products were applied to the paper-ba ... | 2014 | 24973541 |
| pregnancy-associated listeriosis: clinical characteristics and geospatial analysis of a 10-year period in israel. | listeria monocytogenes is a foodborne pathogen that causes life-threatening infections in elderly, immunocompromised, and pregnant women. in pregnancy it may cause fetal loss or a preterm delivery, and the neonate is prone to neonatal sepsis and death. | 2014 | 24973315 |
| evaluation of the removal and destruction effect of a chlorine and thiamine dilaurylsulfate combined treatment on l. monocytogenes biofilm. | the present study investigated the efficacy of single and combined treatment of both chlorine and thiamine dilaurylsulfate (tds) on the reduction of listeria monocytogenes biofilms in microtiter plate. the disinfectants used in this study were 50, 100, and 200 mg/l chlorine and 100, 500, and 1000 mg/l of tds. biofilm-forming index (bfi) and culturable cell count were used to evaluate the disinfectant assay. the highest bfi reduction was 0.80, achieved by the combination of 200 mg/l chlorine and ... | 2014 | 24971770 |
| listeria monocytogenes induces ifnβ expression through an ifi16-, cgas- and sting-dependent pathway. | listeria monocytogenes is a gram-positive facultative intracellular bacterium, which replicates in the cytoplasm of myeloid cells. interferon β (ifnβ) has been reported to play an important role in the mechanisms underlying listeria disease. although studies in murine cells have proposed the bacteria-derived cyclic-di-amp to be the key bacterial immunostimulatory molecule, the mechanism for ifnβ expression during l. monocytogenes infection in human myeloid cells remains unknown. here we report t ... | 2014 | 24970844 |
| an unusual presentation of listeriosis: anemia and cutaneous manifestations. | listeria monocytogenes is an intracellular pathogen causing food-borne disease. it usually affects the young as well as immunocompromised individuals and is associated with high mortality rates. cutaneous manifestations have rarely been described. we describe an interesting case of a traveller from the tropics presenting with cutaneous listeriosis and anemia. | 2014 | 24968680 |
| misidentification of listeria monocytogenes by the vitek 2 system. | | 2014 | 24966361 |
| listeria monocytogenes brainstem infection (rhombencephalitis) mimicking ischemic stroke. | the frequency of listeria monocytogenes (lm) infection of the central nervous system is increasing. we report a patient recently treated with chemotherapeutic drugs for pulmonary adenocarcinoma who suddenly developed hemiparesis, was initially diagnosed with stroke, and was then found to be affected by lm rhombencephalitis accompanied by a brain abscess. lm meningoencephalitis mimicking ischemic stroke is rare but must be considered, especially in specific patients. | 2014 | 24961733 |
| transfusion-related listeria monocytogenes infection in a patient with acute myeloid leukaemia. | | 2014 | 24960659 |
| listeria monocytogenes-associated meningitis and arthritis in an immunocompetent 65-year-old woman: a case report. | we report an unusual case of concurrent meningitis and infectious monoarthritis due to listeria monocytogenes in a 65-year-old woman presenting to our department with a transient gastrointestinal tract illness. during hospitalization the patient's neurological status deteriorated, presenting signs of meningeal irritation along with signs of inflammation and oedema of the right knee. blood cultures and cultures of the cerebrospinal fluid and of the sinovial fluid aspirate showed growth of l. mono ... | 2014 | 24955800 |
| mycotic abdominal aortic aneurysm from listeria monocytogenes. | | 2014 | 24954294 |
| genetic characterization of plasmid-associated triphenylmethane reductase in listeria monocytogenes. | the enzyme triphenylmethane reductase (tmr) reduces toxic triphenylmethane dyes into colorless, nontoxic derivatives, and tmr-producing microorganisms have been proposed as bioremediation tools. analysis of the genome of listeria monocytogenes h7858 (1998-1999 hot dog outbreak) revealed that the plasmid (plm80) of this strain harboring a gene cassette (bcrabc) conferring resistance to benzalkonium chloride (bc) and other quaternary ammonium disinfectants also harbored a gene (tmr) highly homolog ... | 2014 | 24951782 |
| the polycomb repressive complex 2 governs life and death of peripheral t cells. | differentiation of naïve cd4(+) t cells into effector (th1, th2, and th17) and induced regulatory (itreg) t cells requires lineage-specifying transcription factors and epigenetic modifications that allow appropriate repression or activation of gene transcription. the epigenetic silencing of cytokine genes is associated with the repressive h3k27 trimethylation mark, mediated by the ezh2 or ezh1 methyltransferase components of the polycomb repressive complex 2 (prc2). here we show that silencing o ... | 2014 | 24951427 |
| a fast, reliable, and sensitive method for detection and quantification of listeria monocytogenes and escherichia coli o157:h7 in ready-to-eat fresh-cut products by mpn-qpcr. | in the present work we developed a mpn quantitative real-time pcr (mpn-qpcr) method for a fast and reliable detection and quantification of listeria monocytogenes and escherichia coli o157:h7 in minimally processed vegetables. in order to validate the proposed technique, the results were compared with conventional mpn followed by phenotypic and biochemical assays methods. when l. monocytogenes and e. coli o157:h7 were artificially inoculated in fresh-cut vegetables, a concentration as low as 1 c ... | 2014 | 24949460 |
| use carum copticum essential oil for controlling the listeria monocytogenes growth in fish model system. | this study was conducted to evaluate the antibacterial effect of carum copticum essential oil (ajowan eo) against listeria monocytogenes in fish model system. ajowan eo chemical composition was determined by gas chromatography/mass spectral analysis and the highest concentration of carum copticum essential oil without any significant changes on sensory properties of kutum fish (rutilus frisii kutum) was assigned. then the inhibitory effect of ajowan eo at different concentrations in presence of ... | 2014 | 24948918 |
| control of listeria monocytogenes growth in soft cheeses by bacteriophage p100. | the purpose of this study was to determine the effect of bacteriophage p100 on strains of listeria monocytogenes in artificially inoculated soft cheeses. a mix of l. monocytogenes 1/2a and scott a was inoculated in minas frescal and coalho cheeses (approximately 10(5) cfu/g) with the bacteriophage added thereafter (8.3 × 10(7) pfu/g). samples were analyzed immediately, and then stored at 10 °c for seven days. at time zero, 30 min post-infection, the bacteriophage p100 reduced l. monocytogenes co ... | 2014 | 24948908 |
| role of host gtpases in infection by listeria monocytogenes. | the bacterial pathogen listeria monocytogenes induces internalization into mammalian cells and uses actin-based motility to spread within tissues. listeria accomplishes this intracellular life cycle by exploiting or antagonizing several host gtpases. internalization into human cells is mediated by the bacterial surface proteins inla or inlb. these two modes of uptake each require a host actin polymerization pathway comprised of the gtpase rac1, nucleation promotion factors, and the arp2/3 comple ... | 2014 | 24948362 |
| toxoplasma gondii profilin promotes recruitment of ly6chi ccr2+ inflammatory monocytes that can confer resistance to bacterial infection. | ly6c+ inflammatory monocytes are essential to host defense against toxoplasma gondii, listeria monocytogenes and other infections. during t. gondii infection impaired inflammatory monocyte emigration results in severe inflammation and failure to control parasite replication. however, the t. gondii factors that elicit these monocytes are unknown. early studies from the remington laboratory showed that mice with a chronic t. gondii infection survive lethal co-infections with unrelated pathogens, i ... | 2014 | 24945711 |
| listeria exploits damage and death to spread bad news. | pathogenic listeria monocytogenes replicates within the host cytosol; little is known about how it transits from cell to cell, spreading infection. a recent study implicates infection-induced membrane damage as a trigger for efferocytosis, the recognition and uptake of dead cells, thereby tricking neighboring cells into taking up the invader. | 2014 | 24934861 |
| [bacteriostatic effect and/or xylitol bactericide of crops on listeria monocytogenes]. | listeria monocytogenes has been considered as an emerging pathogen causing foodborne illness. in the search for an alternate route biocontrol propagation, xylitol has been proposed as a possible bacteriostatic and / or bactericide. xylitol is a polyol derived from the hydrogenation of xylose monosaccharide of importance in the pharmaceutical industry for its anti-cariogenic effect. to check the possible effect of xylitol as bacteriostatic and/or bactericidal against listeria monocytogenes, it wa ... | 2013 | 24934074 |
| synergistic effects of ultrasound and sodium hypochlorite (naocl) on reducing listeria monocytogenes atcc19118 in broth, stainless steel, and iceberg lettuce. | this study was performed in order to determine whether a combined treatment of ultrasound and sodium hypochlorite (naocl) is more effective than individual treatment on reducing listeria monocytogenes atcc19118 on stainless steel and iceberg lettuce. the bactericidal effect of ultrasound and naocl was investigated in tryptic soy broth (tsb), on stainless steel and iceberg lettuce. various concentrations of naocl (50, 100, 150, and 200 ppm) were tested along with various ultrasound treatment time ... | 2014 | 24927252 |
| building a molecular listeria monocytogenes database to centralize and share pfge typing data from food, environmental and animal strains throughout europe. | the european union reference laboratory (eurl) for listeria monocytogenes (lm) collaborates with a network of 35 national reference laboratories (nrls) throughout europe. most of these nrls are in charge of detecting and typing lm strains from food, environment and animals, which are isolated nationally. the past few years eurl activities have enabled nrls to reinforce typing capabilities according to standardised protocols. consequently the need to exchange typing data within the nrl network ha ... | 2014 | 24918990 |
| establishment of a simple and rapid identification method for listeria spp. by using high-resolution melting analysis, and its application in food industry. | listeria monocytogenes is the causative bacteria of listeriosis, which has a higher mortality rate than that of other causes of food poisoning. listeria spp., of which l. monocytogenes is a member, have been isolated from food and manufacturing environments. several methods have been published for identifying listeria spp.; however, many of the methods cannot identify newly categorized listeria spp. additionally, they are often not suitable for the food industry, owing to their complexity, cost, ... | 2014 | 24918440 |
| sting ligand c-di-gmp improves cancer vaccination against metastatic breast cancer. | cancer vaccination may be our best and most benign option for preventing or treating metastatic cancer. however, breakthroughs are hampered by immune suppression in the tumor microenvironment. in this study, we analyzed whether cyclic diguanylate (c-di-gmp), a ligand for stimulator of interferon genes (sting), could overcome immune suppression and improve vaccination against metastatic breast cancer. mice with metastatic breast cancer (4t1 model) were therapeutically immunized with an attenuated ... | 2014 | 24913717 |
| enhanced survival during experimental listeria monocytogenes sepsis in neonatal mice prophylactically treated with th1 and macrophage immunoregulatory cytokines and mediators. | impairments in t-cell and macrophage-mediated host defense lead to increased infection-related morbidity and mortality in neonates, partly because of immaturity in t helper (th)1 function. listeria monocytogenes (lm) is an intracellular pathogen disproportionately causing severe disease among neonates and the immunocompromised. intact macrophage and th1-mediated immune responses are critical for lm clearance. we and others have previously demonstrated downregulation of th1 and macrophage immunor ... | 2014 | 24911893 |
| a trip in the "new microbiology" with the bacterial pathogen listeria monocytogenes. | listeria monocytogenes is a food-borne pathogen causing an opportunistic disease called listeriosis. this bacterium invades and replicates in most cell types, due to its multiple strategies to exploit host molecular mechanisms. research aiming at unravelling listeria invasion and intracellular lifestyle has led to a number of key discoveries in infection biology, cell biology and also microbiology. in this review, we report on our most recent advances in understanding the intimate crosstalk betw ... | 2014 | 24911203 |
| congenic mice reveal genetic epistasis and overlapping disease loci for autoimmune diabetes and listeriosis. | the nonobese diabetic (nod) mouse strain serves as a genomic standard for assessing how allelic variation for insulin-dependent diabetes (idd) loci affects the development of autoimmune diabetes. we previously demonstrated that c57bl/6 (b6) mice harbor a more diabetogenic allele than nod mice for the idd14 locus when introduced onto the nod genetic background. new congenic nod mouse strains, harboring smaller b6-derived intervals on chromosome 13, now localize idd14 to an ~18-mb interval and rev ... | 2014 | 24906421 |
| enhanced bactericidal action of acidified sodium chlorite caused by the saturation of reactants. | factors affecting the antibacterial action of acidified sodium chlorite (asc), a widely used disinfectant, have not been determined. this study investigated the significant factors suggesting efficient production method to maximize bactericidal action of asc. | 2014 | 24905216 |
| cell-wall deficient l. monocytogenes l-forms feature abrogated pathogenicity. | stable l-forms are cell wall-deficient bacteria which are able to multiply and propagate indefinitely, despite the absence of a rigid peptidoglycan cell wall. we investigated whether l-forms of the intracellular pathogen l. monocytogenes possibly retain pathogenicity, and if they could trigger an innate immune response. while phagocytosis of l. monocytogenes l-forms by non-activated macrophages sometimes resulted in an unexpected persistence of the bacteria in the phagocytes, they were effective ... | 2014 | 24904838 |
| listeria monocytogenes meningoencephalitis in adults: analysis of factors related to unfavourable outcome. | to analyse the short-term outcome in patients with listeria monocytogenes meningoencephalitis (lmme) to improve management and outcome. | 2014 | 24902522 |
| effect of cinnamon (cinnamomum zeylanicum) essential oil supplementation on lamb growth performance and meat quality characteristics. | a trial was conducted to examine the effect of cinnamon essential oil supplementation on lamb growth performance and meat quality. sixteen male lambs were randomly assigned to two groups. the first group served as control and was given a basal diet, and the second group was given the same diet supplemented with cinnamon oil (1 ml/kg of concentrated feed) for 35 days. incorporation of cinnamon oil did not affect growth performance (p>0.05). meat ph, colour, water-holding capacity, shear force, in ... | 2014 | 24902083 |
| quantum dots and graphene oxide fluorescent switch based multivariate testing strategy for reliable detection of listeria monocytogenes. | graphene oxide (go) and quantum dots (qds), as burgeoning types of nanomaterials, have gained tremendous interest in the biosensor field. in this work, we designed a novel multivariate testing strategy that depends on the fluorescence resonance energy transfer (fret) effect between quantum dots (qds) and graphene oxide (go). it integrates the qd-go fret principle and qd probes with different emission peaks into a platform, aims at multiplex gene detection of a human infectious and highly pathoge ... | 2014 | 24901065 |
| dietary pyridoxine controls efficacy of vitamin b6-auxotrophic tuberculosis vaccine bacillus calmette-guérin δurec::hly δpdx1 in mice. | the only tuberculosis (tb) vaccine in use today, bacillus calmette-guérin (bcg), provides insufficient protection and can cause adverse events in immunocompromised individuals, such as bcgosis in hiv(+) newborns. we previously reported improved preclinical efficacy and safety of the recombinant vaccine candidate bcg δurec::hly, which secretes the pore-forming listeriolysin o of listeria monocytogenes. here, we evaluate a second-generation construct, bcg δurec::hly δpdx1, which is deficient in py ... | 2014 | 24895310 |
| classification of listeria monocytogenes persistence in retail delicatessen environments using expert elicitation and machine learning. | increasing evidence suggests that persistence of listeria monocytogenes in food processing plants has been the underlying cause of a number of human listeriosis outbreaks. this study extracts criteria used by food safety experts in determining bacterial persistence in the environment, using retail delicatessen operations as a model. using the delphi method, we conducted an expert elicitation with 10 food safety experts from academia, industry, and government to classify l. monocytogenes persiste ... | 2014 | 24888445 |
| cultivated strains of agaricus bisporus and a. brasiliensis: chemical characterization and evaluation of antioxidant and antimicrobial properties for the final healthy product--natural preservatives in yoghurt. | agaricus bisporus (j. e. lange) emil j. imbach and agaricus brasiliensis wasser, m. didukh, amazonas & stamets are edible mushrooms. we chemically characterized these mushrooms for nutritional value, hydrophilic and lipophilic compounds. the antioxidant and antimicrobial activities of methanolic and ethanolic extracts were assessed. hepatotoxicity was also evaluated. the ethanolic extract of both species was tested for inhibition of listeria monocytogenes growth in yoghurt. both species proved t ... | 2014 | 24881564 |
| combined effects of chlorine and thiamine dilauryl sulfate on reduction of listeria monocytogenes in chicken breast and development of predictive growth models. | the inhibitory effect of chlorine (50, 100, and 200 ml/kg) and thiamine dilauryl sulfate (tds: 100, 500, and 1,000 mg/kg) on listeria monocytogenes in chicken breast was investigated. also, predictive growth models as a function of chlorine and tds concentration, and storage temperature (4, 10, and 15°c) were developed using a polynomial model. listeria monocytogenes counts were significantly (p < 0.05) different in samples treated with sterile distilled water and combinations of chlorine and td ... | 2014 | 24879700 |
| microwave-assisted hydrodistillation of essential oil from rosemary. | effects of microwave assisted hydrodistillation (mahd) and conventional hydrodistillation (hd) methods on yield, composition, specific gravity, refractive index, and antioxidant and antimicrobial activities of essential oil of rosmarinus officinalis l were studied. the main aroma compounds of rosemary essential oil were found as 1,8-cineole and camphor. trolox equivalent antioxidant capacity (teac) values for essential oils extracted by mahd and hd were 1.52 mm/ml oil and 1.95 mm/ml oil, respect ... | 2014 | 24876637 |
| [rapid detection of listeria monocytogenes in pork samples by real-time pcr with taqman probe]. | to develop a real-time pcr method for detection listeria monocytogenes in pork samples. | 2014 | 24868965 |
| selective pharmacologic inhibition of a pasta kinase increases listeria monocytogenes susceptibility to β-lactam antibiotics. | while β-lactam antibiotics are a critical part of the antimicrobial arsenal, they are frequently compromised by various resistance mechanisms, including changes in penicillin binding proteins of the bacterial cell wall. genetic deletion of the penicillin binding protein and serine/threonine kinase-associated protein (pasta) kinase in methicillin-resistant staphylococcus aureus (mrsa) has been shown to restore β-lactam susceptibility. however, the mechanism remains unclear, and whether pharmacolo ... | 2014 | 24867981 |
| attenuated listeria monocytogenes: a powerful and versatile vector for the future of tumor immunotherapy. | for over a century, inactivated or attenuated bacteria have been employed in the clinic as immunotherapies to treat cancer, starting with the coley's vaccines in the 19th century and leading to the currently approved bacillus calmette-guérin vaccine for bladder cancer. while effective, the inflammation induced by these therapies is transient and not designed to induce long-lasting tumor-specific cytolytic t lymphocyte (ctl) responses that have proven so adept at eradicating tumors. therefore, in ... | 2014 | 24860789 |
| numerical spatio-temporal characterization of listeria monocytogenes biofilms. | as the structure of biofilms plays a key role in their resistance and persistence, this work presents for the first time the numerical characterization of the temporal evolution of biofilm structures formed by three listeria monocytogenes strains on two types of stainless-steel supports, aisi 304 ss no. 2b and aisi 316 ss no. 2r. counting methods, motility tests, fluorescence microscopy and image analysis were combined to study the dynamic evolution of biofilm formation and structure. image anal ... | 2014 | 24858448 |
| selection and characterization of dna aptamers specific for listeria species. | single-stranded (ss) dna aptamers with binding affinity to listeria spp. were selected using a whole-cell selex (systematic evolution of ligands by exponential enrichment) method. listeria monocytogenes cells were grown at 37°c and harvested at mid-log phase or early stationary phase to serve as the targets in selex. a total of 10 unique aptamer sequences were identified, six associated with log phase cells and four with stationary phase cells. binding affinity of the aptamers was determined usi ... | 2014 | 24857773 |
| release of silver and copper nanoparticles from polyethylene nanocomposites and their penetration into listeria monocytogenes. | since infection is a major cause of death in a patient whose immune responses have been compromised (immunocompromised patient), considerable attention has been focused on developing materials for the prevention of infections. this has been directed primarily at suppressing or eliminating the host's endogenous microbial burden and decreasing the acquisition of new organisms. in this study, the antibacterial properties of two nanocomposites, polyethylene modified with silver nanoparticles (pe-agn ... | 2014 | 24857461 |
| dynamic functional modulation of cd4+ t cell recall responses is dependent on the inflammatory environment of the secondary stimulus. | the parameters that modulate the functional capacity of secondary th1 effector cells are poorly understood. in this study, we employ a serial adoptive transfer model system to show that the functional differentiation and secondary memory potential of secondary cd4+ effector t cells are dependent on the inflammatory environment of the secondary challenge. adoptive transfer of tcr transgenic lymphocytic choriomeningitis virus (lcmv) glycoprotein-specific smarta memory cells into lcmv-immune hosts, ... | 2014 | 24854337 |
| viability of listeria monocytogenes on uncured turkey breast commercially prepared with and without buffered vinegar during extended storage at 4 and 10°c. | we determined the viability of listeria monocytogenes on uncured turkey breast containing buffered vinegar (bv) and surface treated with a stabilized solution of sodium chlorite in vinegar (vsc). commercially produced, uncured, deli-style turkey breast was formulated with bv (0.0, 2.0, 2.5, or 3.0%), sliced (ca. 100 g and ca. 1.25 cm thick), and subsequently surface inoculated (ca. 4.3 log cfu per slice) in each of two trials with a five-strain cocktail of l. monocytogenes. next, 1 ml per side o ... | 2014 | 24853523 |
| infrared sensor-based aerosol sanitization system for controlling escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes on fresh produce. | an economical aerosol sanitization system was developed based on sensor technology for minimizing sanitizer usage, while maintaining bactericidal efficacy. aerosol intensity in a system chamber was controlled by a position-sensitive device and its infrared value range. the effectiveness of the infrared sensor-based aerosolization (isa) system to inactivate escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes on spinach leaf surfaces was compared with conventional aerosoli ... | 2014 | 24853521 |
| antibacterial activity and immune responses of a molluscan macrophage expressed gene-1 from disk abalone, haliotis discus discus. | the membrane-attack complex/perforin (macpf) domain-containing proteins play an important role in the innate immune response against invading microbial pathogens. in the current study, a member of the macpf domain-containing proteins, macrophage expressed gene-1 (mpeg1) encoding 730 amino acids with the theoretical molecular mass of 79.6 kda and an isoelectric point (pi) of 6.49 was characterized from disk abalone haliotis discus discus (abmpeg1). we found that the characteristic macpf domain (v ... | 2014 | 24852343 |
| novel strategies to enforce an epithelial phenotype in mesenchymal cells. | e-cadherin downregulation in cancer cells is associated with epithelial-to-mesenchymal transition (emt) and metastatic prowess, but the underlying mechanisms are incompletely characterized. in this study, we probed e-cadherin expression at the plasma membrane as a functional assay to identify genes involved in e-cadherin downregulation. the assay was based on the e-cadherin-dependent invasion properties of the intracellular pathogen listeria monocytogenes. on the basis of a functional readout, a ... | 2014 | 24845104 |
| [application and evaluation of loop-mediated isothermal amplification method for detecting of listeria monocytogenes in food]. | the loop-mediated isothermal amplification (lamp) detection method was applied to detect listeria monocytogenes in food. the specificity and sensitivity of this method were evaluated through comparing it with real-time pcr and conventional detection method. | 2014 | 24844836 |
| [listeriosis and pregnancy]. | listeria monocytogenes is a ubiquitous gram-positive bacillus widespread in nature. it is responsible for febrile abortion and encephalitis in cattle. it survives and multiplies at 4°c and does not alter the taste of food. listeria monocytogenes is able to cross 3 host barriers: the intestinal, blood-brain and placental barriers. maternal infection is rare but still severe in france, with 30 to 50 annual cases. maternal infection is frequently asymptomatic, or manifests as isolated fever, wherea ... | 2014 | 24836302 |
| optimization of a real time pcr based method for the detection of listeria monocytogenes in pork meat. | the aim of this study was to optimize a real-time pcr protocol for a rapid detection of listeria monocytogenes in pork meat, using reduced volumes of primary selective enrichment broth and times of incubation to decrease the cost and time for analysis. forty-five samples of pork meat were artificially contaminated with two different levels of l. monocytogenes (1-10 cfu per sample and 10-100 cfu per sample), homogenized in three different volumes of half fraser broth (1:3; 1:5 and 1:10) and incub ... | 2014 | 24835318 |
| the function of the chemokine receptor cxcr6 in the t cell response of mice against listeria monocytogenes. | the chemokine receptor cxcr6 is expressed on different t cell subsets and up-regulated following t cell activation. cxcr6 has been implicated in the localization of cells to the liver due to the constitutive expression of its ligand cxcl16 on liver sinusoidal endothelial cells. here, we analyzed the role of cxcr6 in cd8+ t cell responses to infection of mice with listeria monocytogenes. cd8+ t cells responding to listerial antigens acquired high expression levels of cxcr6. however, deficiency of ... | 2014 | 24832098 |
| evaluation of vidas listeria monocytogenes xpress (lmx) for the detection of listeria monocytogenes in a variety of foods: first action 2013.11. | the vidas listeria monocytogenes xpress (lmx) is an automated rapid screening enzyme immunoassay for the detection of listeria monocytogenes in food products. the vidas lmx method was compared in a multi-laboratory collaborative study to aoac official method 993.12 listeria monocytogenes in milk and dairy products reference method following current aoac guidelines. a total of 14 laboratories participated, representing government and industry, throughout the united states. one matrix, queso fresc ... | 2014 | 24830157 |
| evaluation of vidas up listeria assay (lpt) for the detection of listeria in a variety of foods and environmental surfaces: first action 2013.10. | the vidas up listeria (lpt) is an automated rapid screening enzyme phage-ligand based assay for the detection of listeria species in human food products and environmental samples. the vidas lpt method was compared in a multi-laboratory collaborative study to aoac official method 993.12 listeria monocytogenes in milk and dairy products reference method following current aoac guidelines. a total of 14 laboratories participated, representing government and industry, throughout the united states. on ... | 2014 | 24830156 |
| colonization of plants by human pathogenic bacteria in the course of organic vegetable production. | in recent years, increasing numbers of outbreaks caused by the consumption of vegetables contaminated with human pathogenic bacteria were reported. the application of organic fertilizers during vegetable production is one of the possible reasons for contamination with those pathogens. in this study laboratory experiments in axenic and soil systems following common practices in organic farming were conducted to identify the minimal dose needed for bacterial colonization of plants and to identify ... | 2014 | 24829562 |
| prevalence and contamination patterns of listeria monocytogenes in flammulina velutipes plants. | four mushroom (flammulina velutipes) production plants were sampled to investigate the prevalence and contamination source of listeria monocytogenes. among 295 samples, the prevalence of l. monocytogenes was 18.6%; the contamination appeared to originate from the mycelium-scraping machinery, contaminating both the product and upstream packaging equipment. of 55 l. monocytogenes isolates, lineages i.1 (1/2a-3a) and ii.2 (1/2b-3b-7) accounted for 65.5% and 34.5%, respectively. in addition, lineage ... | 2014 | 24824447 |
| investigation of listeria, salmonella, and toxigenic escherichia coli in various pet foods. | the veterinary laboratory investigation and response network (vet-lirn), in collaboration with the food emergency response network (fern) and its microbiology cooperative agreement program (mcap) laboratories, conducted a study to evaluate the prevalence of selected microbial organisms in various types of pet foods. the goal of this blinded study was to help the center for veterinary medicine prioritize potential future pet food-testing efforts. the study also increased the fern laboratories' sc ... | 2014 | 24824368 |
| investigating the control of listeria monocytogenes on a ready-to-eat ham product using natural antimicrobial ingredients and postlethality interventions. | ready-to-eat (rte) meat and poultry products manufactured with natural or organic methods are at greater risk for listeria monocytogenes growth, if contaminated, than their conventional counterparts due to the required absence of preservatives and antimicrobials. thus, the objective of this study was to investigate the use of commercially available natural antimicrobials and postlethality interventions in the control of l. monocytogenes growth and recovery on a rte ham product. antimicrobials ev ... | 2014 | 24824223 |
| what is new in listeriosis? | listeriosis is a disease caused by listeria monocytogenes (l. monocytogenes). l. monocytogenes is bacteria that usually infects some determined inhabitants, especially high risk patients such as the elderly, immunosuppressed patients and pregnant women. however, it can also affect people who do not have these risk factors. l. monocytogenes is widespread in nature being part of the faecal flora of many mammals and it is a common foodborne source. it is acquired by humans primarily through consump ... | 2014 | 24822197 |
| role of tumor suppressor tsc1 in regulating antigen-specific primary and memory cd8 t cell responses to bacterial infection. | the serine/threonine kinase mammalian/mechanistic target of rapamycin (mtor) integrates various environmental cues such as the presence of antigen, inflammation, and nutrients to regulate t cell growth, metabolism, and function. the tuberous sclerosis 1 (tsc1)/tsc2 complex negatively regulates the activity of an mtor-containing multiprotein complex called mtor complex 1. recent studies have revealed an essential cell-intrinsic role for tsc1 in t cell survival, quiescence, and mitochondrial homeo ... | 2014 | 24818661 |
| staphylococcus pseudintermedius for camp-test. | camp test reliably detects listeria monocytogenes (lm) and streptococcus agalactiae (group b streptococcus, gbs); it is traditionally performed streaking the tested isolate perpendicularly to staphylococcus aureus (sa), provided that reference sa strains (that produce β-hemolysin) are used. in a zone of β-hemolysin activity, in fact, gbs and lm form typical arrow-shaped hemolytic areas. while sa production of the toxin is strain-dependent, however, that of staphylococcus pseudintermedius (sp), a ... | 2014 | 24817971 |