| complete genome sequence of listeria monocytogenes strain dpc6895, a serotype 1/2b isolate from bovine raw milk. | listeria monocytogenes is a foodborne pathogen and is the causative agent of listeriosis among humans and animals. the draft genome sequence of l. monocytogenes dpc6895, a serotype 1/2b strain isolated from the raw milk of a cow with subclinical bovine mastitis, is reported. | 2015 | 26067969 |
| expression of virulence-related genes in listeria monocytogenes grown on danish hard cheese as affected by nacl content. | expression of virulence-related genes in listeria monocytogenes incubated on cheese was assessed by real-time quantitative polymerase chain reaction. the objective of the study was to investigate the impact of sodium chloride concentration in cheese on transcription of virulence genes and, thereby, virulence potential of l. monocytogenes. the expression studies were performed with l. monocytogenes strains characterized by different tolerance to salt stress. strains atcc(®) 51779 and dsmz 15675 w ... | 2015 | 26067229 |
| assessing bacterial invasion of cardiac cells in culture and heart colonization in infected mice using listeria monocytogenes. | listeria monocytogenes is a gram-positive facultative intracellular pathogen that is capable of causing serious invasive infections in immunocompromised patients, the elderly, and pregnant women. the most common manifestations of listeriosis in humans include meningitis, encephalitis, and fetal abortion. a significant but much less documented sequelae of invasive l. monocytogenes infection involves the heart. the death rate from cardiac illness can be up to 35% despite treatment, however very li ... | 2015 | 26065439 |
| activation of prfa results in overexpression of virulence factors but does not rescue the pathogenicity of listeria monocytogenes m7. | listeria monocytogenes encodes a transcriptional activator, prfa, to positively regulate the expression of virulence factors. several mutations in prfa (prfa*) have been found to contribute to increased regulatory activity. here, we describe a strain, m7, containing a prfa*(g145s) that activates expression of virulence factors but with low pathogenicity. to study this contradictory relationship, we exchanged the prfa genes between strains egde and m7 (designated egde-prfa(m7) and m7-prfa(egde)). ... | 2015 | 26055558 |
| inactivation of escherichia coli o157:h7 and listeria monocytogenes in biofilms by pulsed ultraviolet light. | the inactivation of biofilms formed by pathogenic bacteria on ready-to-eat and minimally processed fruits and vegetables by nonthermal processing methods is critical to ensure food safety. pulsed ultraviolet (puv) light has shown promise in the surface decontamination of liquid, powdered, and solid foods. in this study, the antimicrobial efficacy of puv light treatment on nascent biofilms formed by escherichia coli o157:h7 and listeria monocytogenes on the surfaces of food packaging materials, s ... | 2015 | 26054759 |
| atp binding drives substrate capture in an ecf transporter by a release-and-catch mechanism. | ecf transporters are a family of active transporters for vitamins. they are composed of four subunits: a membrane-embedded substrate-binding subunit (ecfs), a transmembrane coupling subunit (ecft) and two atp-binding-cassette atpases (ecfa and ecfa'). we have investigated the mechanism of the ecf transporter for riboflavin from the pathogen listeria monocytogenes, lmecf-ribu. using structural and biochemical approaches, we found that atp binding to the ecfaa' atpases drives a conformational chan ... | 2015 | 26052893 |
| the rab5-gef function of rin1 regulates multiple steps during listeria monocytogenes infection. | | 2015 | 26050999 |
| quantifying viable vibrio parahaemolyticus and listeria monocytogenes simultaneously in raw shrimp. | a novel taqman-based multiplex real-time pcr method combined with propidium monoazide (pma) treatment was firstly developed for the simultaneous quantification of viable vibrio parahaemolyticus and listeria monocytogenes in raw shrimp. the optimization of pma concentration showed that 100 μm was considered optimal to effectively inhibit 10(8) cfu/ml dead cells of both bacteria. the high specificity of this method was confirmed on tests using 96 target and non-target strains. the optimized assay ... | 2015 | 26048473 |
| inactivation of listeria monocytogenes using water bath heat treatment in vacuum packed ricotta salata cheese wedges. | ricotta salata cheese is frequently contaminated on the surface with listeria monocytogenes. water bath heat treatment in vacuum packed whole ricotta salata cheese wheels demonstrated to be effective in inactivating l. monocytogenes. however, the risk of cross-contamination in ricotta salata wedges is increased during cheese cutting. therefore, the effectiveness of heat treatment in ricotta salata wedges has to be demonstrated conducting a new validation study. in this study, 9 different time te ... | 2015 | 26044297 |
| effect of milk fat content on the performance of ohmic heating for inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium and listeria monocytogenes. | the effect of milk fat content on ohmic heating compared to conventional heating for inactivation of food-borne pathogens was investigated. | 2015 | 26043029 |
| structural analysis and molecular docking of potential ligands with chorismate synthase of listeria monocytogenes: a novel antibacterial drug target. | listeriosis, in particular that caused by listeria monocytogenes, is a major foodborne pathogen, and its control is becoming difficult because of widespread emergence of drug resistance strains. chorismate synthase (cs), an essential enzyme of shikimate pathway present only in bacteria, fungi, plant and some apicomplexan parasites, is a validated potential antimicrobial drug target. antimicrobial development through the elucidation of essential structural features of the cs of l. monocytogenes ( ... | 2015 | 26040111 |
| comparative effects of ohmic and conventional heating for inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes in skim milk and cream. | ohmic heating has proven advantages over conventional thermal processing and novel thermal alternative technologies. in this study, the effect of ohmic and conventional heating for pasteurizing skim milk and cream was examined. all treatment conditions for ohmic and conventional heating were identical except for composition of the heating chamber. in most cases, the reduction of three pathogens did not differ significantly between ohmic heating and conventional heating at fixed treatment tempera ... | 2015 | 26038915 |
| effect of acidified sorbate solutions on the lag-phase durations and growth rates of listeria monocytogenes on meat surfaces. | the surfaces of ready-to-eat meats are susceptible to postprocessing contamination by listeria monocytogenes. this study quantified the lag-phase durations (lpd) and growth rates (gr) of l. monocytogenes on the surfaces of cooked ham as affected by sorbate solutions of different concentrations and ph levels. slices of cooked ham inoculated with a four-strain mixture of l. monocytogenes (ca. 10(3) cfu/g) were surface treated with sorbate solutions of 0 to 4% (wt/vol) at ph 4.0 to 6.5, vacuum pack ... | 2015 | 26038906 |
| comparative study on the efficacy of bacteriophages, sanitizers, and uv light treatments to control listeria monocytogenes on sliced mushrooms (agaricus bisporus). | the following reports on a comparative study on the efficacy of different decontamination technologies to decrease listeria monocytogenes inoculated onto white sliced mushrooms and assesses the fate of residual levels during posttreatment storage under aerobic conditions at 8 °c. the treatments were chemical (hydrogen peroxide, peroxyacetic acid, ozonated water, electrolyzed water, chitosan, lactic acid), biological (listeria bacteriophages), and physical (uv-c, uv-hydrogen peroxide). none of th ... | 2015 | 26038905 |
| growth of salmonella enterica and listeria monocytogenes on fresh-cut cantaloupe under different temperature abuse scenarios. | effective cold chain management is a critical component of food safety practice. in this study, we examined the impact of commonly encountered temperature abuse scenarios on the proliferation of salmonella enterica and listeria monocytogenes on fresh-cut cantaloupe. inoculated fresh-cut cantaloupe cubes were subjected to various temperature abuse conditions, and the growth of s. enterica and l. monocytogenes was determined. during 1 week of storage, salmonella cell counts on fresh-cut cantaloupe ... | 2015 | 26038902 |
| a case report of listeria monocytogenes abscesses presenting as cortically predominant ring-enhancing lesions. | listeria monocytogenes, a common cause of bacterial meningitis, rarely involves the central nervous system (cns) in the form of multiple cerebral ring-enhancing lesions. | 2017 | 26034484 |
| the elimination of listeria monocytogenes attached to stainless steel or aluminum using multiple hurdles. | ready-to-eat luncheon meats sliced in retail delis have been found to pose the greatest risk of foodborne illness from listeria monocytogenes among all ready-to-eat foods. slicers used in delis have many removable parts that are connected with seals and gaskets, with spaces, cracks and crevices that are difficult to clean adequately and may provide a niche for l. monocytogenes survival. standard cleaning and sanitizing practices used by deli employees may not eliminate listeria in these niches. ... | 2015 | 26033521 |
| inactivation of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in apple juice at different ph levels by gaseous ozone treatment. | we investigated the effect of ozone treatment of apple juice at different ph levels for inactivation of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes. | 2015 | 26033289 |
| synergistic effect of slightly acidic electrolyzed water and ultrasound at mild heat temperature in microbial reduction and shelf-life extension of fresh-cut bell pepper. | the objectives of this study were to evaluate the effect of combined treatments (slightly acidic electrolyzed water (saew), ultrasound (us), or mild heat (60°c)) on the growth of listeria monocytogenes and salmonella enterica serovar typhimurium in fresh-cut bell pepper, and the shelf-life and sensory quality (color and texture) were followed during storage at 4°c and 25°c. an additional 0.65, 1.72, and 2.70 log cfu/g reduction was achieved by heat treatments at 60°c for 1 min for dw, saew, and ... | 2015 | 26032362 |
| the pamp c-di-amp is essential for listeria monocytogenes growth in rich but not minimal media due to a toxic increase in (p)ppgpp. [corrected]. | cyclic di-adenosine monophosphate (c-di-amp) is a widely distributed second messenger that appears to be essential in multiple bacterial species, including the gram-positive facultative intracellular pathogen listeria monocytogenes. in this study, the only l. monocytogenes diadenylate cyclase gene, daca, was deleted using a cre-lox system activated during infection of cultured macrophages. all δdaca strains recovered from infected cells harbored one or more suppressor mutations that allowed grow ... | 2015 | 26028365 |
| the efficiency of uvc radiation in the inactivation of
listeria monocytogenes on beef-agar food models. | the aim of this study is to evaluate the effect of meat content and surface smoothness on the deactivation of listeria monocytogenes in beef-agar food models achieved by shortwave ultraviolet (uvc) light. food models with various meat contents were made using chopped beef slices and agar solution. prepared models together with a listeria selective agar (lsa) plate and a slice of cooked beef were inoculated with l. monocytogenes and then exposed to uvc light. population of listeria reduced to bel ... | 2015 | 27904353 |
| two variants of selenium-dependent glutathione peroxidase from the disk abalone haliotis discus discus: molecular characterization and immune responses to bacterial and viral stresses. | glutathione peroxidase (gpx) is an essential member of the antioxidant systems of living organisms and may be involved in immune defense against pathogenic invasion. in the current study, two selenium-dependent glutathione peroxidases (absegpxs) that shared 54.3% identity were identified from the disk abalone haliotis discus discus. the open reading frames (orfs) of absegpx-a and absegpx-b coded for 222 and 220 amino acids, respectively, with a characteristic selenocysteine residue encoded by an ... | 2015 | 26025184 |
| complete genome sequence of listeria monocytogenes n2306, a strain associated with the 2013-2014 listeriosis outbreak in switzerland. | we present the complete genome sequence of listeria monocytogenes n2306, a serotype 4b clinical strain isolated during the 2013-2014 nationwide listeriosis outbreak in switzerland. | 2015 | 26021930 |
| bacterial spread from cell to cell: beyond actin-based motility. | several intracellular pathogens display the ability to propagate within host tissues by displaying actin-based motility in the cytosol of infected cells. as motile bacteria reach cell-cell contacts they form plasma membrane protrusions that project into adjacent cells and resolve into vacuoles from which the pathogen escapes, thereby achieving spread from cell to cell. seminal studies have defined the bacterial and cellular factors that support actin-based motility. by contrast, the mechanisms s ... | 2015 | 26021574 |
| intracellular listeria monocytogenes comprises a minimal but vital fraction of the intestinal burden following foodborne infection. | listeria monocytogenes is a highly adaptive bacterium that replicates as a free-living saprophyte in the environment as well as a facultative intracellular pathogen that causes invasive foodborne infections. the intracellular life cycle of l. monocytogenes is considered to be its primary virulence determinant during mammalian infection; however, the proportion of l. monocytogenes that is intracellular in vivo has not been studied extensively. in this report, we demonstrate that the majority of w ... | 2015 | 26015479 |
| listeriolysin o mediates cytotoxicity against human brain microvascular endothelial cells. | penetration of the brain microvascular endothelial layer is one of the routes listeria monocytogenes use to breach the blood-brain barrier. because host factors in the blood severely limit direct invasion of human brain microvascular endothelial cells (hbmecs) by l. monocytogenes, alternative mechanisms might be used by this bacterium to penetrate the endothelial cell layer. in this study, we evaluated the cytotoxicity of proteins secreted by l. monocytogenes against hbemcs using a live/dead sta ... | 2015 | 26013565 |
| hygiene and welfare evaluation of pigs slaughtered in agritourisms. | the slaughtering procedures at agritourism farms must be carried out in accordance with the general and hygiene requirements of regulations (ec) no 852 and 853/2004. in addition, regional laws define minimum requirements allowing some flexibility. piglets and finishing pigs are the most frequently slaughtered animal in sardinian agritourism farms. the aim of the present survey was to evaluate: the general and hygiene requirements of outbuilding slaughterhouses in agritourisms; the animal welfare ... | 2015 | 27800393 |
| quantifying strain variability in modeling growth of listeria monocytogenes. | prediction of microbial growth kinetics can differ from the actual behavior of the target microorganisms. in the present study, the impact of strain variability on maximum specific growth rate (μmax) (h(-1)) was quantified using twenty listeria monocytogenes strains. the μmax was determined as function of four different variables, namely ph, water activity (aw)/nacl concentration [nacl], undissociated lactic acid concentration ([ha]), and temperature (t). the strain variability was compared to b ... | 2015 | 26011600 |
| construction of fe3o4/vancomycin/peg magnetic nanocarrier for highly efficient pathogen enrichment and gene sensing. | infectious diseases, especially pathogenic bacterial infections, pose a growing threat to public health worldwide. as pathogenic bacteria usually exist in complex experimental matrixes at very low concentrations, developing a technology for rapid and biocompatible sample enrichment is essential for sensitive diagnosis. in this study, an fe3o4/vancomycin/peg magnetic nanocarrier was constructed for efficient sample enrichment and in situ nucleic acid preparation of pathogenic bacteria for subsequ ... | 2015 | 26005899 |
| survival of listeria monocytogenes in soil requires agra-mediated regulation. | in a recent paper, we demonstrated that inactivation of the agr system affects the patterns of survival of listeria monocytogenes (a.-l. vivant, d. garmyn, l. gal, and p. piveteau, front cell infect microbiol 4:160, http://dx.doi.org/10.3389/fcimb.2014.00160). in this study, we investigated whether the agr-mediated response is triggered during adaptation in soil, and we compared survival patterns in a set of 10 soils. the fate of the parental strain l. monocytogenes l9 (a rifampin-resistant muta ... | 2015 | 26002901 |
| combination treatment of chlorine dioxide gas and aerosolized sanitizer for inactivating foodborne pathogens on spinach leaves and tomatoes. | the objective of this study was to evaluate the antimicrobial effect of chlorine dioxide (clo2) gas and aerosolized sanitizer, when applied alone or in combination, on the survival of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes inoculated onto spinach leaves and tomato surfaces. spinach leaves and tomatoes were inoculated with a cocktail of three strains each of the three foodborne pathogens. clo2 gas (5 or 10 ppmv) and aerosolized peracetic acid (paa) (80 ppm) w ... | 2015 | 26001524 |
| l-rhamnosylation of listeria monocytogenes wall teichoic acids promotes resistance to antimicrobial peptides by delaying interaction with the membrane. | listeria monocytogenes is an opportunistic gram-positive bacterial pathogen responsible for listeriosis, a human foodborne disease. its cell wall is densely decorated with wall teichoic acids (wtas), a class of anionic glycopolymers that play key roles in bacterial physiology, including protection against the activity of antimicrobial peptides (amps). in other gram-positive pathogens, wta modification by amine-containing groups such as d-alanine was largely correlated with resistance to amps. ho ... | 2015 | 26001194 |
| surgical cytoreduction restores the antitumor efficacy of a listeria monocytogenes vaccine in malignant pleural mesothelioma. | recent studies suggest that immunotherapy may offer a promising treatment strategy for early-stage malignant pleural mesothelioma (mpm), but advanced tumor burden may limit the efficacy of immunotherapy. therefore, we hypothesized that surgical cytoreduction could restore the efficacy of vaccine-based immunotherapy for mpm. we developed a murine model of mpm through transduction of a mesothelioma cell line with mesothelin. we used this model to evaluate the efficacy of a listeria monocytogenes v ... | 2015 | 25999306 |
| inactivation dynamics of salmonella enterica, listeria monocytogenes, and escherichia coli o157:h7 in wash water during simulated chlorine depletion and replenishment processes. | maintaining effective sanitizer concentration is of critical importance for preventing pathogen survival and transference during fresh-cut produce wash operation and for ensuring the safety of finished products. however, maintaining an adequate level of sanitizer in wash water can be challenging for processors due to the large organic load in the wash system. in this study, we investigated how the survival of human pathogens was affected by the dynamic changes in water quality during chlorine de ... | 2015 | 25998820 |
| genome-wide sirna screen identifies complementary signaling pathways involved in listeria infection and reveals different actin nucleation mechanisms during listeria cell invasion and actin comet tail formation. | listeria monocytogenes enters nonphagocytic cells by a receptor-mediated mechanism that is dependent on a clathrin-based molecular machinery and actin rearrangements. bacterial intra- and intercellular movements are also actin dependent and rely on the actin nucleating arp2/3 complex, which is activated by host-derived nucleation-promoting factors downstream of the cell receptor met during entry and by the bacterial nucleation-promoting factor acta during comet tail formation. by genome-wide sma ... | 2015 | 25991686 |
| proteomics analysis of listeria monocytogenes atcc 19115 in response to simultaneous triple stresses. | listeria monocytogenes can cause listeriosis in humans through consumption of contaminated food. l. monocytogenes can adapt and grow in a vast array of physiochemical stresses in the food production environment. in this study, we performed a proteomics strategy in order to investigate how l. monocytogenes survives with a simultaneous exposure to low ph, high salinity and low temperature. the results showed that the adaptation processes mainly affected the biochemical pathways related to protein ... | 2015 | 25990453 |
| molecular epidemiology and genetic diversity of listeria monocytogenes isolates from a wide variety of ready-to-eat foods and their relationship to clinical strains from listeriosis outbreaks in chile. | listeria monocytogenes is a pathogen transmitted through food that can cause severe infections in high-risk groups such as pregnant women, elderly, young children and immunocompromised individuals. it is a ubiquitous bacterium that can survive in harsh conditions, such as dry environments, at low temperatures, in brine conditions and at low ph values. it also has the capacity to form biofilms, which makes it particularly successful even in colonizing surfaces within food processing plants. this ... | 2015 | 25983727 |
| antibiotic susceptibility of listeria monocytogenes in argentina. | listeria monocytogenes is the causative agent of listeriosis, a food-borne disease that mainly affects pregnant women, the elderly, and immunocompromised patients. the primary treatment of choice of listeriosis is the combination of ampicillin or penicillin g, with an aminoglycoside, classically gentamicin. the second-choice therapy for patients allergic to β-lactams is the combination of trimethoprim with a sulfonamide (such as co-trimoxazole). the aim of this study was to analyze the antimicro ... | 2016 | 25976753 |
| differences in the expression of cold stress-related genes and in the swarming motility among persistent and sporadic strains of listeria monocytogenes. | the persistence of certain listeria monocytogenes strains in food-related environments suggests niche adaptation of these strains and therefore constitutes a major risk to consumer health and results in economic losses for the food producer. in this study, a set of 23 l. monocytogenes isolates, including a group of persistent and a group of sporadic strains, was evaluated regarding their swarming motility at 11°c. in each group, significant (p<0.05) differences in motility were observed. the tra ... | 2015 | 25974395 |
| combined effects of organic acids and salt depending on type of acids and pathogens in laboratory media and acidified pickle. | in this study, the effectiveness of combining each of seven types of acids with 3% salt as a treatment against pathogens was investigated in laboratory media and acidified food. | 2015 | 25973744 |
| immersion infection of germ-free zebrafish with listeria monocytogenes induces transient expression of innate immune response genes. | zebrafish, denio rerio, can be an alternative to other classic animal models for human infectious diseases to examine the processes of microbial infections and host-pathogen interactions in vivo because of their small body dimension but large clutch size. we established germ-free zebrafish infection models of listeria monocytogenes through different routes of infection: oral immersion and injection via yolk sac, brain ventricle and blood island. immersion of zebrafish larva even with 10(10) cfu/ ... | 2015 | 25972853 |
| cd81 controls immunity to listeria infection through rac-dependent inhibition of proinflammatory mediator release and activation of cytotoxic t cells. | despite recent evidence on the involvement of cd81 in pathogen binding and ag presentation by dendritic cells (dcs), the molecular mechanism of how cd81 regulates immunity during infection remains to be elucidated. to investigate the role of cd81 in the regulation of defense mechanisms against microbial infections, we have used the listeria monocytogenes infection model to explore the impact of cd81 deficiency in the innate and adaptive immune response against this pathogenic bacteria. we show t ... | 2015 | 25972472 |
| strain-specific interactions of listeria monocytogenes with the autophagy system in host cells. | listeria monocytogenes is an intracellular bacterial pathogen that can replicate in the cytosol of host cells. these bacteria undergo actin-based motility in the cytosol via expression of acta, which recruits host actin-regulatory proteins to the bacterial surface. l. monocytogenes is thought to evade killing by autophagy using acta-dependent mechanisms. acta-independent mechanisms of autophagy evasion have also been proposed, but remain poorly understood. here we examined autophagy of non-motil ... | 2015 | 25970638 |
| a rapidly fatal sepsis caused by listeria monocytogenes type-4b in a patient with chronic renal failure. | listeria monocytogenes is a significant zoonosis causing invasive infections in the susceptible persons. the current paper presented a patient who died due to a rapidly-progressing multiple organ failure (mof) as a result of severe sepsis caused by l. monocytogenes. | 2015 | 25969704 |
| expression and function analysis of two naturally truncated myd88 variants in the pacific oyster crassostrea gigas. | myeloid differentiation factor 88 (myd88) is the classic signaling adaptor that mediates toll/interleukin-1 receptor (tir/il-1r) dependent activation of nuclear factor-kappa b (nf-κb). in this study, two naturally truncated myd88 members were identified from the pacific oyster (crassostrea gigas), namely cgmyd88-t1 and cgmyd88-t2. the full-length cdna of cgmyd88-t1, cgmyd88-t2 are 976 bp and 1038 bp in length, containing an orf of 552 bp and 555 bp, respectively. the two orf encode a putative pr ... | 2015 | 25963623 |
| prevalence and antimicrobial susceptibility profile of listeria species from ready-to-eat foods of animal origin in gondar town, ethiopia. | listeriosis, mostly caused by listeria monocytogenes species, has become a major concern to public health authorities due to its clinical severity and high mortality rate, particularly in high risk groups. currently, there is limited information regarding the prevalence and antimicrobial susceptibility profiles of listeria species in ready-to-eat foods of animal origin in gondar town, ethiopia. the aim of this study was to determine the prevalence and antimicrobial susceptibility pattern of list ... | 2015 | 25963134 |
| are nectarines to blame? a case report and literature review of spontaneous bacterial peritonitis due to listeria monocytogenes. | spontaneous bacterial peritonitis (sbp) is a frequent and potentially deadly complication of ascites in patients with end-stage liver disease. unlike other pathogens more commonly implicated in sbp, listeria monocytogenes is a nonenteric organism that maybe acquired either sporadically or in the setting of foodborne outbreaks. listeria is an unusual cause of sbp that presents particular management challenges because of the organism's intrinsic resistance to first-line and empiric sbp treatment t ... | 2015 | 25960571 |
| cheese-related listeriosis outbreak, portugal, march 2009 to february 2012. | in portugal, listeriosis has been notifiable since april 2014, but there is no active surveillance programme for the disease. a retrospective study involving 25 national hospitals led to the detection of an outbreak that occurred between march 2009 and february 2012. the amount of time between the start of the outbreak and its detection was 16 months. of the 30 cases of listeriosis reported, 27 were in the lisbon and vale do tejo region. two cases were maternal/neonatal infections and one result ... | 2015 | 25955775 |
| comparative efficacy of potassium levulinate with and without potassium diacetate and potassium propionate versus potassium lactate and sodium diacetate for control of listeria monocytogenes on commercially prepared uncured turkey breast. | we evaluated the efficacy of potassium levulinate (klev; 0.0, 1.0, 1.5, and 2.0%) with and without a blend of potassium propionate (0.1%) and potassium diacetate (0.1%) (kpd) versus a blend of potassium lactate (1.8%) and sodium diacetate (0.125%) (kld) for inhibiting listeria monocytogenes on commercially prepared, uncured turkey breast during refrigerated storage. product formulated with kld or klev (1.5%) was also subsequently surface treated with 44 ppm of a solution of lauric arginate (lae) ... | 2015 | 25951386 |
| development of predictive models for the growth kinetics of listeria monocytogenes on fresh pork under different storage temperatures. | this study was conducted to develop a predictive model to estimate the growth of listeria monocytogenes on fresh pork during storage at constant temperatures (5, 10, 15, 20, 25, 30, and 35°c). the baranyi model was fitted to growth data (log cfu per gram) to calculate the specific growth rate (sgr) and lag time (lt) with a high coefficient of determination (r(2) > 0.98). as expected, sgr increased with a decline in lt with rising temperatures in all samples. secondary models were then developed ... | 2015 | 25951385 |
| crucial roles of tnfaip8 protein in regulating apoptosis and listeria infection. | tnf-α-induced protein 8 (tnfaip8 or tipe) is a newly described regulator of cancer and infection. however, its precise roles and mechanisms of actions are not well understood. we report in this article that tnfaip8 regulates listeria monocytogenes infection by controlling pathogen invasion and host cell apoptosis in a rac1 gtpase-dependent manner. tnfaip8-knockout mice were resistant to lethal l. monocytogenes infection and had reduced bacterial load in the liver and spleen. tnfaip8 knockdown in ... | 2015 | 25948813 |
| centrifugal step emulsification applied for absolute quantification of nucleic acids by digital droplet rpa. | aqueous microdroplets provide miniaturized reaction compartments for numerous chemical, biochemical or pharmaceutical applications. we introduce centrifugal step emulsification for the fast and easy production of monodisperse droplets. homogenous droplets with pre-selectable diameters in a range from 120 μm to 170 μm were generated with coefficients of variation of 2-4% and zero run-in time or dead volume. the droplet diameter depends on the nozzle geometry (depth, width, and step size) and inte ... | 2015 | 25947077 |
| combined effect of thermosonication and slightly acidic electrolyzed water to reduce foodborne pathogens and spoilage microorganisms on fresh-cut kale. | this study evaluated the efficacy of individual treatments (thermosonication [ts+dw] and slightly acidic electrolyzed water [sacew]) and their combination on reducing escherichia coli o157:h7, listeria monocytogenes, and spoilage microorganisms (total bacterial counts [tbc], enterobacteriaceae, pseudomonas spp., and yeast and mold counts [ymc]) on fresh-cut kale. for comparison, the antimicrobial efficacies of sodium chlorite (sc; 100 mg/l) and sodium hypochlorite (sh; 100 mg/l) were also evalua ... | 2015 | 25944413 |
| improved recovery of listeria monocytogenes from stainless steel and polytetrafluoroethylene surfaces using air/water ablation. | to develop a gentle ablation technique to recover listeria monocytogenes biofilms from stainless steel (ss) and polytetrafluoroethylene (ptfe) surfaces by using compressed air and water injection. | 2015 | 25943582 |
| prevalence of listeria spp. and molecular characterization of listeria monocytogenes isolates from broilers at the abattoir. | products from three broiler abattoirs were sampled for listeria species to evaluate the changes in the prevalence and contamination rates at two stages of processing. sampling was performed at the evisceration stage and at the end of processing after packaging and refrigerating at 4°c for 24 h. a total of 212 samples were collected; 52 were from abattoir a, and 80 samples each were collected from abattoirs b and c. among all samples, 99 (46.7%) tested positive for listeria, including l. monocyto ... | 2015 | 25942617 |
| development of a convenience and safety chilled sous vide fish dish: diversification of aquacultural products. | the dynamic expansion of the ready-to-eat seabream sector in its adaptation to new lifestyles has led to the search for new presentation formats in seabream (sparus aurata). green sauce (olive oil, wine vinegar, garlic, fresh parsley, black pepper, basil and salt) and 60 ℃ of cooking temperature were chosen by the panellists for the sous vide cooking process. seabream fillet and sauce were packaged in polypropylene trays, cooked, chilled and stored at 2 ℃. microbiological (total viable counts,en ... | 2016 | 25941212 |
| a novel mutation leading to a premature stop codon in inla of listeria monocytogenes isolated from neonatal listeriosis. | the study objective was to investigate whether the strain of l. monocytogenes serotype 1/2c isolated from neonatal listeriosis carries a premature stop codon (pmsc) mutation in the inla gene. the strain was characterized by serotyping, macrorestriction analysis after digestion with the restriction enzyme asci, and sequencing of the inla gene. the tested strain of serotype 1/2c and pulsotype 1 possesses a new type of point mutation leading to a pmsc in the inla gene and production of truncated in ... | 2015 | 25938757 |
| tlr2 and ap-1/nf-kappab are involved in the regulation of mmp-9 elicited by heat killed listeria monocytogenes in human monocytic thp-1 cells. | mmp-9 is crucial for a normal immune response, but excessive release of this enzyme leads to severe tissue damage. listeria monocytogenes (lm) is an opportunistic food-borne pathogen causing listerosis, meningitis and sepsis. heat killed listeria monocytogenes (hklm) activates immune system and leads production of cytokines and chemokines. however, nothing is known about the involvement of hklm in mmp-9 regulation. therefore we investigated the role of hklm in the regulation of mmp-9 gene expres ... | 2015 | 25931987 |
| outbreak of encephalitic listeriosis in red-legged partridges (alectoris rufa). | an outbreak of neurological disease was investigated in red-legged partridges between 8 and 28 days of age. clinical signs included torticollis, head tilt and incoordination and over an initial eight day period approximately 30-40 fatalities occurred per day. no significant gross post mortem findings were detected. histopathological examination of the brain and bacterial cultures followed by partial sequencing confirmed a diagnosis of encephalitis due to listeria monocytogenes. further isolates ... | 2015 | 25921827 |
| controlling listeria monocytogenes in cold smoked salmon with the antimicrobial peptide salmine. | listeria monocytogenes (lm) is a major safety concern for smoked salmon producers, as it can survive both the brining and smoking process in cold smoked salmon production. salmine is a cationic antimicrobial peptide derived from the milt of salmon that has been shown to inhibit the growth of lm in vitro. commercialization of this peptide would add value to a waste product produced when raising salmon. the purpose of this study was to determine the anti-listeria activity of salmine in smoked salm ... | 2015 | 25920744 |
| the essential oil profiles and antibacterial activity of six wild cinnamomum species. | the essential oil composition of six species of wild cinnamomum found in borneo was investigated. the oils were obtained from bark by hydrodistillation and the volatile chemical profile was obtained via gas chromatography-mass spectrometry (gcms). a total of 65 volatile constituents were identified, where the essential oils of the studied specimens contained high contents of oxygenated monoterpenes. eucalyptol (1.2-31.1%), terpinen-4-ol (7.9-22.1%), eugenol (0.4-37.9%) and α-cadinol (0.4-1.8%) w ... | 2014 | 25918818 |
| listeriosis outbreaks in british columbia, canada, caused by soft ripened cheese contaminated from environmental sources. | soft ripened cheese (src) caused over 130 foodborne illnesses in british columbia (bc), canada, during two separate listeriosis outbreaks. multiple agencies investigated the events that lead to cheese contamination with listeria monocytogenes (l.m.), an environmentally ubiquitous foodborne pathogen. in both outbreaks pasteurized milk and the pasteurization process were ruled out as sources of contamination. in outbreak a, environmental transmission of l.m. likely occurred from farm animals to pe ... | 2015 | 25918702 |
| three novel clade b serine protease inhibitors from disk abalone, haliotis discus discus: molecular perspectives and responses to immune challenges and tissue injury. | serine protease inhibitors (serpins) control cellular protease activity in order to maintain cellular homeostasis. the immune and inflammatory responses of invertebrate clade b serpins have not been widely reported. in the present study, three proteins with high similarity to clade b serpins, referred to as abserpin-1, abserpin-2 and abserpin-3, were identified from disk abalone (haliotis discus discus). while abserpin-1 (399 aa) was of a typical size for this protein class, abserpin-2 (506 aa) ... | 2015 | 25917971 |
| short communication: latin-style fresh cheese enhances lactic acid bacteria survival but not listeria monocytogenes resistance under in vitro simulated gastrointestinal conditions. | different studies in humans have provided evidence about the health benefits of probiotics. however, most probiotic strains do not maintain good viability in the harsh conditions of the gastrointestinal tract (git). in the present study, latin-style fresh cheese produced with potential probiotic bacteria was tested to evaluate this cheese type as a food carrier for the delivery of viable microorganisms after exposure to simulated git conditions. the resistance of 28 lactic acid bacteria (lab) st ... | 2015 | 25912867 |
| geographical and longitudinal analysis of listeria monocytogenes genetic diversity reveals its correlation with virulence and unique evolution. | listeria monocytogenes is one of the most important foodborne pathogens causing severe diseases with a mortality rate of 24%. however, the genetic diversity and evolution of l. monocytogenes, particularly at the worldwide level, are poorly defined. in this study, we performed multilocus sequence typing (mlst) and multi virulence locus sequence typing (mvlst) for 86 l. monocytogenes strains derived from 8 countries from 1926 to 2012 in order to better understand the molecular evolution and geneti ... | 2015 | 25912377 |
| virr-mediated resistance of listeria monocytogenes against food antimicrobials and cross-protection induced by exposure to organic acid salts. | formulations of ready-to-eat (rte) foods with antimicrobial compounds constitute an important safety measure against foodborne pathogens such as listeria monocytogenes. while the efficacy of many commercially available antimicrobial compounds has been demonstrated in a variety of foods, the current understanding of the resistance mechanisms employed by l. monocytogenes to counteract these stresses is limited. in this study, we screened in-frame deletion mutants of two-component system response r ... | 2015 | 25911485 |
| gene expression in listeria monocytogenes exposed to sublethal concentration of benzalkonium chloride. | in this study, tolerance at sublethal concentration of benzalkonium chloride and transcription levels of mdrl, ladr, lde, sigb and bcrabc genes in listeria monocytogenes strains were evaluated. viable cells reduction occurred in 45% of strains and clinical isolates showed lower sensitivity than isolates from foods. an increased transcription of an efflux system encoding gene was found in 60% of strains, and simultaneous mdrl overexpression and ladr underexpression occurred in 30% of isolates. a ... | 2015 | 25910634 |
| ultrasensitive detection and rapid identification of multiple foodborne pathogens with the naked eyes. | in this study, a novel approach for ultrasensitive detection and rapid high-throughput identification of a panel of common foodborne pathogens with the naked eyes is presented. as a proof-of-concept application, a multiple pathogen analysis array is fabricated through immobilizing three specific polyt-capture probes which can respectively recognize rfbe gene (escherichia coli o157:h7), inva gene (salmonella enterica), inla gene (listeria monocytogenes) on the plastic substrates. pcr has been dev ... | 2015 | 25909338 |
| atlas(®) listeria monocytogenes lmg2 detection assay using transcription mediated amplification to detect listeria monocytogenes in selected foods and stainless steel surface. | the atlas listeria monocytogenes lmg2 detection assay, developed by roka bioscience inc., was compared to a reference culture method for seven food types (hot dogs, cured ham, deli turkey, chicken salad, vanilla ice cream, frozen chocolate cream pie, and frozen cheese pizza) and one surface (stainless steel, grade 316). a 125 g portion of deli turkey was tested using a 1:4 food:media dilution ratio, and a 25 g portion for all other foods was tested using 1:9 food:media dilution ratio. the enrich ... | 2016 | 25902984 |
| bioactive properties of a propolis-based dietary supplement and its use in combination with mild heat for apple juice preservation. | this study characterizes the antioxidant and antibacterial properties of a propolis-based dietary supplement (pds) and investigates its incorporation into apple juice to decrease the intensity of the heat treatment required to inactivate 5 log10 cycles of escherichia coli o157:h7. as the source of propolis, we used a pds containing 0.2 mg/μl of propylene glycol-extracted propolis (propolis). the total phenolic content and antioxidant activity (ic50) of the pds were 82.15±3.53 mg/g and 0.055±0.00 ... | 2015 | 25897992 |
| listeriaphages and coagulin c23 act synergistically to kill listeria monocytogenes in milk under refrigeration conditions. | bacteriophages and bacteriocins are promising biocontrol tools in food. in this work, two listeria bacteriophages, fwllm1 and fwllm3, were assessed in combination with the bacteriocin coagulin c23 to inhibit listeria monocytogenes. preliminary results under laboratory conditions demonstrated that both antimicrobials act synergistically when they were applied in suboptimal concentrations. the combined approach was further assessed in milk contaminated with 5×10(4) cfu/ml l. monocytogenes 2000/47 ... | 2015 | 25897991 |
| oxygen limitation induces acid tolerance and impacts simulated gastro-intestinal transit in listeria monocytogenes j0161. | ᅟ: listeria monocytogenes is a food-borne pathogen and the causative agent of listeriosis, a severe infection to those with a pre-disposition. infections often arise through consumption of contaminated foods, where high intrinsic resistance to food processing practises permit survival and growth. several practises, including refrigeration, acidification and oxygen limitation are ineffective in controlling l. monocytogenes, therefore foods which do not undergo thermal processing, e.g. ready-to-ea ... | 2015 | 25897327 |
| host actin polymerization tunes the cell division cycle of an intracellular pathogen. | growth and division are two of the most fundamental capabilities of a bacterial cell. while they are well described for model organisms growing in broth culture, very little is known about the cell division cycle of bacteria replicating in more complex environments. using a d-alanine reporter strategy, we found that intracellular listeria monocytogenes (lm) spend a smaller proportion of their cell cycle dividing compared to lm growing in broth culture. this alteration to the cell division cycle ... | 2015 | 25892235 |
| selection and characterization of phage-resistant mutant strains of listeria monocytogenes reveal host genes linked to phage adsorption. | listeria-infecting phages are readily isolated from listeria-containing environments, yet little is known about the selective forces they exert on their host. here, we identified that two virulent phages, lp-048 and lp-125, adsorb to the surface of listeria monocytogenes strain 10403s through different mechanisms. we isolated and sequenced, using whole-genome sequencing, 69 spontaneous mutant strains of 10403s that were resistant to either one or both phages. mutations from 56 phage-resistant mu ... | 2015 | 25888172 |
| biological effects of listeriolysin o: implications for vaccination. | listeriolysin o (llo) is a thiol-activated cholesterol-dependent pore-forming toxin and the major virulence factor of listeria monocytogenes (lm). extensive research in recent years has revealed that llo exerts a wide array of biological activities, during the infection by lm or by itself as recombinant antigen. the spectrum of biological activities induced by llo includes cytotoxicity, apoptosis induction, endoplasmic reticulum stress response, modulation of gene expression, intracellular calci ... | 2015 | 25874208 |
| antimicrobial coatings with dual cationic and n-halamine character: characterization and biocidal efficacy. | a method to prepare an antimicrobial coating for food-handling materials is reported. alternating layers of branched polyethylenimine and styrene maleic anhydride copolymer were applied onto the surface of polypropylene. the resulting coatings had low surface energy and presented enhanced antimicrobial character due to the presence of both cationic and n-halamine forming structures. in its unchlorinated form, the coating inactivated listeria monocytogenes by ∼3 logarithmic cycles. in the form of ... | 2015 | 25871333 |
| a duplex qpcr for the simultaneous detection of escherichia coli o157:h7 and listeria monocytogenes using lna probes. | in this study, a duplex qpcr assay was developed for the needs of the irish fish industry to screen for the two major food-borne pathogens of fish, listeria monocytogenes and escherichia coli o157:h7. the assay can claim positive or negative results for two pathogens in one go in only 20 h including 16 h universal pre-enrichment and compared to traditional iso approved plate culture methods the labour and the cost involved in testing of one sample is reduced to minimum. the highly specific genom ... | 2015 | 25869996 |
| contrasting regulation of macrophage iron homeostasis in response to infection with listeria monocytogenes depending on localization of bacteria. | due to its multiple roles for the proliferation and pathogenicity of many microbes on the one hand and via modulation of immune effector functions on the other hand the control over iron homeostasis is thought to play a decisive role in the course of infections. diversion of cellular iron traffic is considered as an important defense mechanism of macrophages to reduce metal availability for intracellular bacteria residing in the phagosome. however, evidence is lacking whether such alterations of ... | 2015 | 25869778 |
| development of a real-time loop-mediated isothermal amplification assay for the sensitive and rapid detection of listeria monocytogenes. | a real-time loop-mediated isothermal amplification (realamp) assay for the detection of listeria was developed. the realamp assay, using primers specific for the hemolysin-encoding hlya gene, was verified using listeria monocytogenes strains (n = 58) from different regions of the world. both the sensitivity and specificity of the realamp assay were high. the realamp assay could detect 10(3) cfu ml(-1) within 30 min. a comparative evaluation of the realamp assay, the api listeria assay, and the r ... | 2015 | 25868481 |
| cell-autonomous responses in listeria monocytogenes infection. | listeria monocytogenes is a facultative intracellular bacterium causing listeriosis, a food-borne infection with a high mortality rate. the mechanisms and the role of cells and tissular components in generating protective adaptive immune responses are well studied, and cell biological studies provide a detailed understanding of the processes targeted by the bacterial products. much less is known of the cellular responses activated to limit infection in individual cells when confronted with stres ... | 2015 | 25865195 |
| is there interaction between gut microbial profile and cardiovascular risk in chronic kidney disease patients? | to evaluate the gut microbial profile in chronic kidney disease (ckd) patients and evaluate the possible relationship with inflammation and cardiovascular risk. | 2015 | 25865191 |
| pulsed-field gel electrophoresis for listeria monocytogenes. | pulsed-field gel electrophoresis (pfge) is a molecular subtyping method with high discriminatory power, reproducibility, and epidemiological concordance for the subtyping of listeria monocytogenes and other bacteria. pfge uses rare-cutting restriction enzymes (macrorestriction) that cut the genomic dna, usually resulting in 6-25 dna fragments ranging between 30 and 600 kb. bacterial cells are immobilized in agarose plugs and subsequently lysed to release genomic dna, which is then subjected to d ... | 2015 | 25862047 |
| pfge as a tool to track listeria monocytogenes in food processing facilities: case studies. | the use of pfge to track listeria monocytogenes strains from the food processing environment to the food is explained in this chapter through two case studies. this illustrates the usefulness of this method to identify putative routes of contamination and persistent strains and to help in the implementation of corrective actions in food processing facilities to control the occurrence of this pathogenic bacterium. | 2015 | 25862045 |
| harmonization of pfge profile analysis by using bioinformatics tools: example of the listeria monocytogenes european union reference laboratory network. | standardization in comparison and interpretation of profiles is an integral part of the analysis of gels from pulsed-field gel electrophoresis. using listeria monocytogenes as an example, this chapter outlines the analytical process using the software bionumerics. | 2015 | 25862044 |
| listeria monocytogenes: a model pathogen to study antigen-specific memory cd8 t cell responses. | memory cd8 t cells play a critical role in providing protection to immune hosts by orchestrating rapid elimination of pathogen-infected cells after re-infection. systemic bacterial infection with listeria monocytogenes has been a favored approach for researchers to characterize pathogen-specific cd8 t cell responses, and in-depth understanding of l. monocytogenes biology has provided invaluable experimental tools that have been used to increase our understanding of memory cd8 t cell differentiat ... | 2015 | 25860798 |
| complete genome sequences of vb_lmos_188 and vb_lmos_293, two bacteriophages with specificity for listeria monocytogenes strains of serotypes 4b and 4e. | listeria monocytogenes is responsible for the rare disease listeriosis, which is associated with the consumption of contaminated food products. we report here the complete genome sequences of vb_lmos_188 and vb_lmos_293, phages isolated from environmental sources and that have host specificity for l. monocytogenes strains of the 4b and 4e serotypes. | 2015 | 25858822 |
| multiplexed detection of foodborne pathogens based on magnetic particles. | this paper addresses the novel approaches for the multiplex detection of food poisoning bacteria, paying closer attention to three of the most common pathogens involved in food outbreaks: salmonella enterica, escherichia coli o157:h7 and listeria monocytogenes. end-point and real-time pcr, classical immunological techniques, biosensors, microarrays and microfluidic platforms, as well as commercial kits for multiplex detection of food pathogens will be reviewed, with special focus on the role of ... | 2015 | 25858812 |
| effect of sodium-alginate and laminaran on salmonella typhimurium infection in human enterocyte-like ht-29-luc cells and balb/c mice. | brown algal polysaccharides such as alginate, polymers of uronic acids, and laminaran, beta-1,3 and 1,6-glucan, can be fermented by human intestinal microbiota. to evaluate the effects of these polysaccharides on infections caused by food poisoning pathogens, we investigated the adhesion and invasion of pathogens (salmonella typhimurium, listeria monocytogenes and vibrio parahaemolyticus) in human enterocyte-like ht-29-luc cells and in infections caused in balb/c mice. both sodium na-alginate an ... | 2015 | 25857966 |
| plasticity of listeriolysin o pores and its regulation by ph and unique histidine [corrected]. | pore formation of cellular membranes is an ancient mechanism of bacterial pathogenesis that allows efficient damaging of target cells. several mechanisms have been described, however, relatively little is known about the assembly and properties of pores. listeriolysin o (llo) is a ph-regulated cholesterol-dependent cytolysin from the intracellular pathogen listeria monocytogenes, which forms transmembrane β-barrel pores. here we report that the assembly of llo pores is rapid and efficient irresp ... | 2015 | 25854672 |
| farm to fork quantitative risk assessment of listeria monocytogenes contamination in raw and pasteurized milk cheese in ireland. | the objective of this study was to model and quantify the level of listeria monocytogenes in raw milk cheese (rmc) and pasteurized milk cheese (pmc) from farm to fork using a bayesian inference approach combined with a quantitative risk assessment. the modeling approach included a prediction of contamination arising from the farm environment as well from cross-contamination within the cheese-processing facility through storage and subsequent human exposure. the model predicted a high concentrati ... | 2015 | 25850713 |
| odor fingerprinting of listeria monocytogenes recognized by spme-gc-ms and e-nose. | microorganisms can produce species-specific microbial volatile organic compounds (mvocs), or odor compounds, which can be characterized by odor fingerprinting. the objective of this study was to characterize the odor fingerprint of listeria monocytogenes. solid-phase microextraction-gas chromatography-mass spectrometry (spme-gc-ms) and electronic nose (e-nose) were used to recognize the mvocs of l. monocytogenes in pure culture medium. the main mvocs of l. monocytogenes were identified by spme-g ... | 2015 | 25847596 |
| stochastic modelling of listeria monocytogenes single cell growth in cottage cheese with mesophilic lactic acid bacteria from aroma producing cultures. | a stochastic model was developed for simultaneous growth of low numbers of listeria monocytogenes and populations of lactic acid bacteria from the aroma producing cultures applied in cottage cheese. during more than two years, different batches of cottage cheese with aroma culture were analysed for ph, lactic acid concentration and initial concentration of lactic acid bacteria. these data and bootstrap sampling were used to represent product variability in the stochastic model. lag time data wer ... | 2015 | 25847186 |
| effect of filling type and heating method on prevalence of listeria species and listeria monocytogenes in dumplings produced in poland. | the count of listeria monocytogenes was determined, before and after heat treatment, in 200 samples of dumplings of 9 brands and with different types of stuffing. analyses were conducted according to iso 11290-1 standard and with real-time pcr method. the highest count of l. monocytogenes was found in meat dumplings (10(2) to 10(4) cfu/g), whereas products with white cheese-potato stuffing and vegetable-mushroom stuffing contained significantly less listeria, 20 to 80 and 5 to 32 cfu/g, respecti ... | 2015 | 25847074 |
| effect of sulfur dioxide fumigation on survival of foodborne pathogens on table grapes under standard storage temperature. | we examined the fate of listeria monocytogenes, escherichia coli o157:h7, and salmonella enterica thompson inoculated on freshly-harvested table grapes under standard cold storage with initial and weekly sulfur dioxide (so2) fumigation. l. monocytogenes and s. enterica thompson were much more sensitive to cold temperature than e. coli o157:h7. furthermore, l. monocytogenes was highly susceptible to so2. initial fumigation with 100 or 200 ppm-hr was sufficient to eliminate this pathogen on grapes ... | 2015 | 25846930 |
| effect of ripeness stage during processing on listeria monocytogenes growth on fresh-cut 'conference' pears. | there are several factors that affect the shelf life of fresh-cut fruit, including the cultivar, the ripeness stage of the fruit during processing and the fruit's storage atmosphere and temperature. the effect of fruit ripeness during processing on the survival and growth of listeria monocytogenes on fresh-cut 'conference' pear slices at different temperatures (5, 10 and 20 °c) was studied. the four ripeness stages studied in this work (assessed by a fruit's firmness) were mature-green (54-60 n) ... | 2015 | 25846921 |
| management of listeria monocytogenes in fermented sausages using the food safety objective concept underpinned by stochastic modeling and meta-analysis. | in the present work, a demonstration is made on how the risk from the presence of listeria monocytogenes in fermented sausages can be managed using the concept of food safety objective (fso) aided by stochastic modeling (bayesian analysis and monte carlo simulation) and meta-analysis. for this purpose, the icmsf equation was used, which combines the initial level (h0) of the hazard and its subsequent reduction (σr) and/or increase (σi) along the production chain. each element of the equation was ... | 2015 | 25846913 |
| predicting pathogen-specific cd8 t cell immune responses from a modeling approach. | the primary cd8 t cell immune response constitutes a major mechanism to fight an infection by intra-cellular pathogens. we aim at assessing whether pathogen-specific dynamical parameters of the cd8 t cell response can be identified, based on measurements of cd8 t cell counts, using a modeling approach. we generated experimental data consisting in cd8 t cell counts kinetics during the response to three different live intra-cellular pathogens: two viruses (influenza, vaccinia) injected intranasall ... | 2015 | 25846273 |
| two-component-system histidine kinases involved in growth of listeria monocytogenes egd-e at low temperatures. | two-component systems (tcss) aid bacteria in adapting to a wide variety of stress conditions. while the role of tcs response regulators in the cold tolerance of the psychrotrophic foodborne pathogen listeria monocytogenes has been demonstrated previously, no comprehensive studies showing the role of tcs histidine kinases of l. monocytogenes at low temperature have been performed. we compared the expression levels of each histidine kinase-encoding gene of l. monocytogenes egd-e in logarithmic gro ... | 2015 | 25841007 |
| aerobic plate counts and atp levels correlate with listeria monocytogenes detection in retail delis. | listeria monocytogenes is a foodborne pathogen that causes an estimated 1,591 cases of illness and 255 deaths annually in the united states, the majority of which are attributed to ready-to-eat deli meats processed in retail delis. because retail delis distribute product directly to consumers, rapid methods to validate cleaning and sanitation are needed to improve retail food safety. this study investigated the relationships among atp levels, standard aerobic plate count (apc), and l. monocytoge ... | 2015 | 25836412 |
| growth inhibition of listeria monocytogenes, salmonella enterica, and escherichia coli o157:h7 by d-tryptophan as an incompatible solute. | under osmotic stress, bacterial cells uptake compatible solutes such as glycine-betaine to maintain homeostasis. it is unknown whether incompatible solutes exist that are similar in structure to compatible solutes but have adverse physiological effects on bacterial physiology. the objective of this study was to evaluate solute incompatibility of various amino acids against bacterial growth. bacterial growth was evaluated by changes in optical density at 595 nm in peptone-yeast-glucose (pyg) brot ... | 2015 | 25836411 |