| biodegradation of 4-aminobenzenesulfonate by ralstonia sp. pba and hydrogenophaga sp. pbc isolated from textile wastewater treatment plant. | a co-culture consisting of hydrogenophaga sp. pbc and ralstonia sp. pba, isolated from textile wastewater treatment plant could tolerate up to 100 mm 4-aminobenzenesulfonate (4-abs) and utilize it as sole carbon, nitrogen and sulfur source under aerobic condition. the biodegradation of 4-abs resulted in the release of nitrogen and sulfur in the form of ammonium and sulfate respectively. ninety-eight percent removal of chemical oxygen demand attributed to 20 mm of 4-abs in cell-free supernatant c ... | 2010 | 21094980 |
| automatic analysis of macroarrays images. | the analysis of dot blot (macroarray) images is currently based on the human identification of positive/negative dots, which is a subjective and time consuming process. this paper presents a system for the automatic analysis of dot blot images, using a pre-defined grid of markers, including a number of on and off controls. the geometric deformations of the input image are corrected, and the individual markers detected, both tasks fully automatically. based on a previous training stage, the proba ... | 2010 | 21097139 |
| complete biodegradation of 4-fluorocinnamic acid by a consortium comprising arthrobacter sp. strain g1 and ralstonia sp. strain h1. | a consortium of the newly isolated bacterial strains arthrobacter sp. strain g1 and ralstonia sp. strain h1 utilized 4-fluorocinnamic acid for growth under aerobic conditions. strain g1 converted 4-fluorocinnamic acid into 4-fluorobenzoic acid and used the two-carbon side chain for growth, with some formation of 4-fluoroacetophenone as a dead-end side product. in the presence of strain h1, complete mineralization of 4-fluorocinnamic acid and release of fluoride were obtained. degradation of 4-fl ... | 2010 | 21097599 |
| autoacetylation of the ralstonia solanacearum effector popp2 targets a lysine residue essential for rrs1-r-mediated immunity in arabidopsis. | type iii effector proteins from bacterial pathogens manipulate components of host immunity to suppress defence responses and promote pathogen development. in plants, host proteins targeted by some effectors called avirulence proteins are surveyed by plant disease resistance proteins referred to as "guards". the ralstonia solanacearum effector protein popp2 triggers immunity in arabidopsis following its perception by the rrs1-r resistance protein. here, we show that popp2 interacts with rrs1-r in ... | 2010 | 21124938 |
| cloning of the chlorothalonil-degrading gene cluster and evidence of its horizontal transfer. | strain ochrobactrum lupine tp-d1 was found to degrade chlorothalonil (tpn) to 4-hydroxy-chlorothalonil (tpn-oh). to clone the related degrading gene, genomic library of tp-d1 was constructed using escherichia coli dh10b and two positive clones 889 and 838 were gained. however, no plasmid was detected in clone 889. and in clone 838, a 3494 bp fragment was cloned which contains a 984 bp hydrolytic dehalogenase (chd) gene and a 1926 bp insertion element is-olup. the insertion element contains a tra ... | 2010 | 21127873 |
| ralstonia pickettii community-acquired pneumonia in cambodia. | | 2010 | 21144257 |
| extension of the substrate utilization range of ralstonia eutropha strain h16 by metabolic engineering to include mannose and glucose. | the gram-negative facultative chemolithoautotrophic bacterium ralstonia eutropha strain h16 is known for its narrow carbohydrate utilization range, which limits its use for biotechnological production of polyhydroxyalkanoates and possibly other products from renewable resources. to broaden its substrate utilization range, which is for carbohydrates and related compounds limited to fructose, n-acetylglucosamine, and gluconate, strain h16 was engineered to use mannose and glucose as sole carbon so ... | 2010 | 21169447 |
| dioxygenases in burkholderia ambifaria and yersinia pestis that hydroxylate the outer kdo unit of lipopolysaccharide. | several gram-negative pathogens, including yersinia pestis, burkholderia cepacia, and acinetobacter haemolyticus, synthesize an isosteric analog of 3-deoxy-d-manno-oct-2-ulosonic acid (kdo), known as d-glycero-d-talo-oct-2-ulosonic acid (ko), in which the axial hydrogen atom at the kdo 3-position is replaced with oh. here we report a unique kdo 3-hydroxylase (kdoo) from burkholderia ambifaria and yersinia pestis, encoded by the bamb_0774 (bakdoo) and the y1812 (ypkdoo) genes, respectively. when ... | 2010 | 21178073 |
| improved polyhydroxybutyrate (phb) production in transgenic tobacco by enhancing translation efficiency of bacterial phb biosynthetic genes. | polyhydroxybutyrate [p(3hb)] was produced in the transgenic tobacco harboring the genes encoding acetoacetyl-coa reductase (phab) and polyhydroxyalkanoate synthase (phac) from ralstonia eutropha (cupriavidus necator) with optimized codon usage for expression in tobacco. p(3hb) contents in the transformants (0.2mg/g dry cell weight in average) harboring the codon-optimized phab gene was twofold higher than the control transformants harboring the wild-type phab gene. the immunodetection revealed a ... | 2010 | 21185778 |
| ralstonia solanacearum virulence increased following large interstrain gene transfers by natural transformation. | horizontal gene transfer (hgt) is a major driving force of evolution and is also likely to play an important role in the threatening emergence of novel pathogens, especially if it involves distantly related strains with substantially different pathogenicity. in this study, the impact of natural transformation on pathogenicity in six strains belonging to the four phylotypes of the plant-pathogenic bacterium ralstonia solanacearum was investigated. the study focused on genomic regions that vary be ... | 2011 | 21190441 |
| development and characterization of an enzymatic method for the rapid determination of gamma hydroxybutyric acid. | gamma hydroxybutyric acid (ghb) is a regulated therapeutic drug, which naturally occurs in mammalian brain tissues as an intermediate of the gaba (gamma aminobutyric acid) neurotransmitter metabolism. the increasing misuse of ghb as a narcotic or abusing drug in recent years calls for the development of a simple and rapid screening method as an alternative to the currently available, technically demanding diagnostic methods. we have developed a rapid enzymatic assay based on the ghb dehydrogenas ... | 2010 | 21197843 |
| genetic diversity of ralstonia solanacearum strains from china assessed by pcr-based fingerprints to unravel host plant- and site-dependent distribution patterns. | bacterial wilt caused by ralstonia solanacearum is a serious threat to crop production in china. a collection of 319 r. solanacearum strains isolated from 14 different diseased host plants collected in 15 chinese provinces was investigated by box fingerprints in order to test the influence of the site and the host plant on their genetic diversity. phylotype, flic-rflp patterns and biovar were determined for all strains and the sequevar for 39 representative strains. the majority of strains belon ... | 2011 | 21204873 |
| improved detergent-based recovery of polyhydroxyalkanoates (phas). | extracting polyhydroxyalkanoate (pha) polymer from bacterial cells often involves harsh conditions, including use of environmentally harmful solvents. we evaluated different detergents under various conditions to extract pha from ralstonia eutropha and escherichia coli cells. most detergents tested recovered highly pure pha polymer from cells in amounts that depended on the percentage of polymer present in the cell. detergents such as linear alkylbenzene sulfonic acid (las-99) produced a high yi ... | 2011 | 21207109 |
| structure of ralstonia eutropha flavohemoglobin in complex with three antibiotic azole compounds. | flavohemoglobins (flavohbs) are enzymes that operate primarily as nitric oxide dioxygenases and shuttle thereby electrons among nad(p)h, fad, heme, and a ligated redox-active substrate such as o(2). they function in the bacterial defense against nitrosative stress and are therefore considered as targets for new antibiotic drugs. recently, azole derivatives were proven to be attractive nitric oxide dioxygenase inhibitors, and to explore their binding characteristics, we determined the x-ray struc ... | 2011 | 21210640 |
| high-accuracy prediction of bacterial type iii secreted effectors based on position-specific amino acid composition profiles. | bacterial type iii secreted (t3s) effectors are delivered into host cells specifically via type iii secretion systems (t3sss), which play important roles in the interaction between bacteria and their hosts. previous computational methods for t3s protein prediction have only achieved limited accuracy, and distinct features for effective t3s protein prediction remain to be identified. | 2011 | 21233168 |
| prhklm genes of ralstonia solanacearum encode novel activators of hrp regulon and are required for pathogenesis in tomato. | the genes in the hrp regulon encode the proteins composing type iii secretion system in ralstonia solanacearum. the hrp regulon is positively controlled by hrpb, and hrpb expression is activated by both hrpg and prhg. we have identified three genes, prhk, prhl, and prhm, which positively control the hrp regulon in strain oe1-1. these genes are likely to form an operon, and this operon is well conserved in the genera ralstonia and burkholderia. this indicates that the operon is not specific to th ... | 2011 | 21241356 |
| ralstonia solanacearum extracellular polysaccharide is a specific elicitor of defense responses in wilt-resistant tomato plants. | ralstonia solanacearum, which causes bacterial wilt of diverse plants, produces copious extracellular polysaccharide (eps), a major virulence factor. the function of eps in wilt disease is uncertain. leading hypotheses are that eps physically obstructs plant water transport, or that eps cloaks the bacterium from host plant recognition and subsequent defense. tomato plants infected with r. solanacearum race 3 biovar 2 strain uw551 and tropical strain gmi1000 upregulated genes in both the ethylene ... | 2011 | 21253019 |
| [characteristics of proteins synthesized by hydrogen-oxidizing microorganisms]. | the study was conducted to determine the biological value of proteins synthesized by hydrogen-oxidizing microorganisms--the hydrogen bacteria alcaligenes eutrophus z1 and ralstonia eutropha b5786 and the co-resistant strain of carboxydobacterium seliberia carboxydohydrogena z1062. based on a number of significant parameters characterizing the biological value of a product, the proteins of hydrogen-oxidizing microorganisms have been found to occupy an intermediate position between traditional ani ... | 2010 | 21261071 |
| c-terminal region of plant ferredoxin-like protein is required to enhance resistance to bacterial disease in arabidopsis thaliana. | protein phosphorylation is an important biological process associated with elicitor-induced defense responses in plants. in a previous report, we described how plant ferredoxin-like protein (pflp) in transgenic plants enhances resistance to bacterial pathogens associated with the hypersensitive response (hr). pflp possesses a putative casein kinase ii phosphorylation (ck2p) site at the c-terminal in which phosphorylation occurs rapidly during defense response. however, the contribution of this s ... | 2011 | 21261469 |
| application of nitroarene dioxygenases in the design of novel strains that degrade chloronitrobenzenes. | widespread application of chloronitrobenzenes as feedstocks for the production of industrial chemicals and pharmaceuticals has resulted in extensive environmental contamination with these toxic compounds, where they pose significant risks to the health of humans and wildlife. while biotreatment in general is an attractive solution for remediation, its effectiveness is limited with chloronitrobenzenes due to the small number of strains that can effectively mineralize these compounds and their abi ... | 2009 | 21261918 |
| hydrogen yield from a hydrogenase in frankia r43 at different levels of the carbon source propionate. | fermentative hydrogen yield was investigated in the frankia strain r43, which was grown in different amounts of the carbon source propionate. in relation to hydrogen yield, the hydrogenase enzyme was characterized by use of western blot. a bioreactor study revealed a 10-fold increase in growth within 50 h. the study showed that there is an active anaerobic hydrogen production in frankia r43 and that this hydrogenase is immunologically related to the subunit hoxu of ralstonia eutropha. | 2011 | 21276650 |
| proteomic and transcriptomic elucidation of the mutant ralstonia eutropha g+1 with regard to glucose utilization. | by taking advantage of the available genome sequence of ralstonia eutropha h16, glucose uptake in the uv-generated glucose-utilizing mutant r. eutropha g(+)1 was investigated by transcriptomic and proteomic analyses. data revealed clear evidence that glucose is transported by a usually n-acetylglucosamine-specific phosphotransferase system (pts)-type transport system, which in this mutant is probably overexpressed due to a derepression of the encoding nag operon by an identified insertion mutati ... | 2011 | 21278273 |
| iodide accumulation by aerobic bacteria isolated from subsurface sediments of a 129i-contaminated aquifer at the savannah river site, south carolina. | (129)i is of major concern because of its mobility in the environment, excessive inventory, toxicity (it accumulates in the thyroid), and long half-life (~16 million years). the aim of this study was to determine if bacteria from a (129)i-contaminated oxic aquifer at the f area of the u.s. department of energy's savannah river site, sc, could accumulate iodide at environmentally relevant concentrations (0.1 µm i(-)). iodide accumulation capability was found in 3 out of 136 aerobic bacterial stra ... | 2011 | 21278282 |
| growth and polyhydroxybutyrate production by ralstonia eutropha in emulsified plant oil medium. | polyhydroxyalkanoates (phas) are natural polyesters synthesized by bacteria for carbon and energy storage that also have commercial potential as bioplastics. one promising class of carbon feedstocks for industrial pha production is plant oils, due to the high carbon content of these compounds. the bacterium ralstonia eutropha accumulates high levels of pha and can effectively utilize plant oil. growth experiments that include plant oil, however, are difficult to conduct in a quantitative and rep ... | 2011 | 21279345 |
| versatile metabolic adaptations of ralstonia eutropha h16 to a loss of pdhl, the e3 component of the pyruvate dehydrogenase complex. | a previous study reported that the tn5-induced poly(3-hydroxybutyric acid) (phb)-leaky mutant ralstonia eutropha h1482 showed a reduced phb synthesis rate and significantly lower dihydrolipoamide dehydrogenase (dhldh) activity than the wild-type r. eutropha h16 but similar growth behavior. insertion of tn5 was localized in the pdhl gene encoding the dhldh (e3 component) of the pyruvate dehydrogenase complex (pdhc). taking advantage of the available genome sequence of r. eutropha h16, observation ... | 2011 | 21296938 |
| differential gene expression in arachis diogoi upon interaction with peanut late leaf spot pathogen, phaeoisariopsis personata and characterization of a pathogen induced cyclophilin. | the wild relatives of peanut are resistant to various economically important diseases including late leaf spot (lls) caused by phaeoisariopsis personata, compared with the susceptible cultivated peanut (arachis hypogaea l.). the interaction of the late leaf spot pathogen, phaeoisariopsis personata and the highly resistant, diploid peanut wild species, arachis diogoi was analyzed at the molecular level by differential gene expression studies. genes up-regulated with in 48 h of pathogen challenge ... | 2011 | 21298396 |
| new detection systems of bacteria using highly selective media designed by smart: selective medium-design algorithm restricted by two constraints. | culturing is an indispensable technique in microbiological research, and culturing with selective media has played a crucial role in the detection of pathogenic microorganisms and the isolation of commercially useful microorganisms from environmental samples. although numerous selective media have been developed in empirical studies, unintended microorganisms often grow on such media probably due to the enormous numbers of microorganisms in the environment. here, we present a novel strategy for ... | 2011 | 21304596 |
| characterization of acc deaminase-producing endophytic bacteria isolated from copper-tolerant plants and their potential in promoting the growth and copper accumulation of brassica napus. | one hundred cu-resistant-endophytic bacteria were isolated from cu-tolerant plants grown on cu mine wasteland, of which, eight cu-resistant and 1-aminocyclopropane-1-carboxylate (acc) deaminase-producing endophytic bacteria were obtained based on the acc deaminase activity of the bacteria and characterized with respect to metal resistance, production of acc deaminase, indole-3-acetic acid (iaa) as well as siderophores and mineral phosphate solubilization. ralstonia sp. j1-22-2, pantoea agglomera ... | 2011 | 21315404 |
| [screening, identification, and biocontrol effect of antagonistic bacteria against phytophthora capsici]. | a total of 98 isolates with antagonistic activity against phytophthora capsici were isolated from the rhizosphere soil of healthy pepper plants in the fields seriously infected by pepper phytophthora capsicit, and two strains named as hl-3 and lz-8 were screened, which had the characteristics of wide-spectrum antagonism and good growth under poor soil condition. the hl-3 and lz-8 were identified as paenibacillus polymyxa and bacillus pumilus, respectively, based on their morphological and bioche ... | 2010 | 21328956 |
| production of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by ralstonia eutropha from soybean oil. | high-yield production of polyhydroxyalkanoates (phas) by ralstonia eutropha kctc 2662 was investigated using soybean oil and ?-butyrolactone as carbon sources. in flask culture, it was shown that r. eutropha kctc 2662 accumulated phas during the growth phase. the optimum carbon to nitrogen ratio (c/n ratio) giving the highest cell and pha yield was 20g-soybean oil/g-(nh(4))(2)so(4). the 4-hydroxybutyrate (4hb) fraction in the copolymer was not strongly affected by the c/n ratio. in a 2.5-l ferme ... | 2011 | 21333767 |
| elucidation of the regulon and cis-acting regulatory element of hrpb, the arac-type regulator of a plant pathogen-like type iii secretion system in burkholderia pseudomallei. | the human pathogen burkholderia pseudomallei possesses multiple type iii secretion system (t3ss) gene clusters. one of these, the b. pseudomallei t3ss2 (t3ss2(bp)) gene cluster, which apparently plays no role in animal virulence, is also found in six additional burkholderia spp. and is very similar to t3sss found in phytopathogenic xanthomonas spp. and ralstonia solanacearum. the t3ss2(bp) gene cluster also encodes an arac-type regulatory protein (hrpb(bp)) that is an ortholog of hrpb, the maste ... | 2011 | 21335458 |
| gradually accumulating beneficial mutations to improve the thermostability of n-carbamoyl-d-amino acid amidohydrolase by step-wise evolution. | to further enhance repeated batch reactions with immobilized n-carbamoyl-d-amino acid amidohydrolase (dcase), which can be used for the industrial production of d-amino acids, the stability of high soluble mutant dcase-m3 from ralstonia pickettii cgmcc1596 was improved by step-wise evolution. in our previous report, six thermostability-related sites were identified by error-prone pcr. based on the above result, an improved mutant b5 (q12l/q23l/h248q/t262a/t263s) was obtained through two rounds o ... | 2011 | 21360152 |
| endo- and exopolygalacturonases of ralstonia solanacearum are inhibited by polygalacturonase-inhibiting protein (pgip) activity in tomato stem extracts. | polygalacturonases (pgs) of wild-type and non-virulent phenotype conversion mutant (pc) strains of ralstonia solanacearum were compared by investigating their activities and their inhibition by polygalacturonase-inhibiting proteins (pgips) from tomato stems. in cultures of wild-type strain toudk2, slimy (s), retarded slimy (rs) and non-slimy (ns) colonies appeared. the conversion of the 's' into the 'rs' colony form coincided with the beginning of pg production. pg activity of the pc strain incr ... | 2011 | 21367611 |
| facultative methylotrophs from the human oral cavity and methylotrophy in strains of gordonia, leifsonia, and microbacterium. | we show that bacteria with methylotrophic potential are ubiquitous in the human mouth microbiota. numerous strains of actinobacteria (brevibacterium, gordonia, leifsonia, microbacterium, micrococcus, rhodococcus) and proteobacteria (achromobacter, klebsiella, methylobacterium, pseudomonas, ralstonia) were isolated, and one strain of each of the eleven genera was studied in detail. these strains expressed enzymes associated with methylotrophic metabolism (methanol, methylamine, and formate dehydr ... | 2011 | 21374057 |
| biodiversity and geochemistry of an extremely acidic, low-temperature subterranean environment sustained by chemolithotrophy. | the geochemical dynamics and composition of microbial communities within a low-temperature (ôê+8.5-¦c), long-abandoned (>ôçâ90 years) underground pyrite mine (cae coch, located in north wales) were investigated. surface water percolating through fractures in the residual pyrite ore body that forms the roof of the mine becomes extremely acidic and iron-enriched due to microbially accelerated oxidative dissolution of the sulfide mineral. water droplets on the mine roof were found to host a very li ... | 2011 | 21382147 |
| a novel alkaline lipase from ralstonia with potential application in biodiesel production. | with the aim of isolating a biocatalyst able to catalyze biodiesel production from microbial source, ralstonia sp. cs274 was isolated and a lipase from the strain (rl74) was purified. molecular weight of rl74 was estimated to be 28,000 da by sds-page. the activity was highest at 50-55-¦c and ph 8.0-9.5 and was stable at ph 7.0-12.0 and up to 45-¦c. it was resistant to oxidizing and reducing agents and the activity was enhanced by detergents. rl74 was 1,3 specific and k(m) and v(max) for p-nitrop ... | 2011 | 21388805 |
| a unique iron-sulfur cluster is crucial for oxygen tolerance of a [nife]-hydrogenase. | hydrogenases are essential for h(2) cycling in microbial metabolism and serve as valuable blueprints for h(2)-based biotechnological applications. however, most hydrogenases are extremely oxygen sensitive and prone to inactivation by even traces of o(2). the o(2)-tolerant membrane-bound [nife]-hydrogenase of ralstonia eutropha h16 is one of the few examples that can perform h(2) uptake in the presence of ambient o(2). here we show that o(2) tolerance is crucially related to a modification of the ... | 2011 | 21390036 |
| tomato rav transcription factor is a pivotal modulator involved in the ap2/erebp-mediated defense pathway. | ralstonia solanacearum is the causal agent of bacterial wilt (bw), one of the most important bacterial diseases worldwide. we used cdna microarray to survey the gene expression profile in transgenic tomato (solanum lycopersicum) overexpressing arabidopsis (arabidopsis thaliana) cbf1 (atcbf1), which confers tolerance to bw. the disease-resistant phenotype is correlated with constitutive expression of the related-to-abi3/vp1 (rav) transcription factor, ethylene-responsive factor (erf) family genes ... | 2011 | 21398258 |
| production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered ralstonia eutropha strains. | the polyhydroxyalkanoate (pha) copolymer poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [p(hb-co-hhx)] has been shown to have potential to serve as a commercial bioplastic. synthesis of p(hb-co-hhx) from plant oil has been demonstrated with recombinant ralstonia eutropha strains expressing heterologous pha synthases capable of incorporating hb and hhx into the polymer. with these strains, however, short-chain-length fatty acids had to be included in the medium to generate pha with high hhx conten ... | 2011 | 21398488 |
| antimicrobial flavonoids from the twigs of populus nigra × populus deltoides. | a bioassay-guided fractionation of the ethyl acetate extract from the twigs of the hybrid poplar 'neva', populus nigra l. × populus deltoides marsh, led to the isolation of three flavonoids, which were identified by means of spectrometric and physicochemical analysis as 5-hydroxy-7-methoxy-flavone (1), 5,7-dihydoxy-flavone (2) and 5,7-dihydroxy-flavonol (3). these compounds were further screened for their antimicrobial activity against plant pathogens, including three bacteria (pseudomonas lachr ... | 2011 | 21416454 |
| a motn mutant of ralstonia solanacearum is hypermotile and has reduced virulence. | ralstonia solanacearum is a soil-borne plant pathogen that causes bacterial wilt disease on many plant species. we previously showed that swimming motility contributes to virulence of this bacterium in the early stages of host invasion and colonization. in this study we identified a new negative regulator of motility, named motn, that is located in a cluster of motility-related genes. a motn mutant was hypermotile both on 0.3% agar motility plates and in rich and minimal medium broth. however, l ... | 2011 | 21421761 |
| ralfuranone biosynthesis in ralstonia solanacearum suggests functional divergence in the quinone synthetase family of enzymes. | ralstonia solanacearum is a destructive crop plant pathogen and produces ralfuranone, i.e., a monophenyl-substituted furanone. extensive feeding experiments with (13)c-labeled l-phenylalanine now proved that all carbon atoms of the heterocycle derive, after deamination, from this aromatic amino acid. a genetic locus was identified which encodes the aminotransferase rald and the furanone synthetase rala. the latter is a tridomain nonribosomal peptide synthetase (nrps)-like enzyme which was charac ... | 2011 | 21439480 |
| outbreak of ralstonia paucula pseudobacteraemia in a paediatric accident and emergency department. | | 2011 | 21440953 |
| the maturation factors hoxr and hoxt contribute to oxygen tolerance of membrane-bound [nife] hydrogenase in ralstonia eutropha h16. | the membrane-bound [nife] hydrogenase (mbh) of ralstonia eutropha h16 undergoes a complex maturation process comprising cofactor assembly and incorporation, subunit oligomerization, and finally twin-arginine-dependent membrane translocation. due to its outstanding o(2) and co tolerance, the mbh is of biotechnological interest and serves as a molecular model for a robust hydrogen catalyst. adaptation of the enzyme to oxygen exposure has to take into account not only the catalytic reaction but als ... | 2011 | 21441514 |
| peroxisomal polyhydroxyalkanoate biosynthesis is a promising strategy for bioplastic production in high biomass crops. | polyhydroxyalkanoates (phas) are bacterial carbon storage polymers with diverse plastic-like properties. pha biosynthesis in transgenic plants is being developed as a way to reduce the cost and increase the sustainability of industrial pha production. the homopolymer polyhydroxybutyrate (phb) is the simplest form of these biodegradable polyesters. plant peroxisomes contain the substrate molecules and necessary reducing power for phb biosynthesis, but peroxisomal phb production has not been explo ... | 2011 | 21447054 |
| an hrpb-dependent but type iii-independent extracellular aspartic protease is a virulence factor of ralstonia solanacearum. | the host specificity of ralstonia solanacearum, the causal organism of bacterial wilt on many solanaceous crops, is poorly understood. to identify a gene conferring host specificity of the bacterium, sl341 (virulent to hot pepper but avirulent to potato) and sl2029 (virulent to potato but avirulent to hot pepper) were chosen as representative strains. we identified a gene, rsa1, from sl2029 that confers avirulence to sl341 in hot pepper. the rsa1 gene encoding an 11.8-kda protein possessed the p ... | 2011 | 21453432 |
| differentiating the growing nosocomial infectious threats ralstonia pickettii and ralstonia insidiosa. | differentiation of the growing nosocomial infectious threats, ralstonia pickettii and ralstonia insidiosa, based on nitrate reduction, desferrioxamine susceptibility, arabinose, n-acetyl-glucosamine and phenylacetate assimilation is described. these tests can be used for preliminary identification of ralstonia pickettii and ralstonia insidiosa resulting in more accurate identification of these species. | 2011 | 21461848 |
| expanding the chiral pool: oxidation of meta-bromobenzoic acid by r. eutrophus b9 allows access to new reaction manifolds. | metabolism of meta-bromobenzoic acid by the blocked mutant ralstonia eutrophus b9 affords an enantiopure dearomatised halodiene-diol which we demonstrate is a versatile chiron for organic synthesis. the presence of the halogen leads to reactivity that is distinct to that observed for the non-halogenated analogue and also serves as a synthetic handle for further functionalisation. | 2011 | 21472181 |
| effects of homologous phosphoenolpyruvate-carbohydrate phosphotransferase system proteins on carbohydrate uptake and poly(3-hydroxybutyrate) accumulation in ralstonia eutropha h16. | seven gene loci encoding putative proteins of the phosphoenolpyruvate-carbohydrate phosphotransferase system (pep-pts) were identified in the genome of ralstonia eutropha h16 by in silico analysis. except the n-acetylglucosamine-specific pep-pts, an additional complete pep-pts is lacking in strain h16. based on these findings, we generated single and multiple deletion mutants defective mainly in the pep-pts genes to investigate their influence on carbon source utilization, growth behavior, and p ... | 2011 | 21478317 |
| [differential expression of genes related to bacterial wilt resistance in peanut (arachis hypogaea l.)]. | peanut bacterial wilt (bw) caused by ralstonia solanacearum is one of the most devastating diseases for peanut production in the world. it is believed that breeding and subsequent planting bw-resistant cultivars of peanut (arachis hypogaea l.) should represent the most effective and economic means of controlling the disease. to illustrate the molecular mechanism of peanut resistant to bw, a bw-resistant cultivar, 'yuanza 9102', and a bw-sensitive one, 'zhonghua 12', were infected with ralstonia ... | 2011 | 21482530 |
| community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis. | abstract: | 2011 | 21486476 |
| biocontrol of ralstonia solanacearum by treatment with lytic bacteriophages. | ralstonia solanacearum is a gram-negative bacterium and the causative agent of bacterial wilt in many important crops. we treated r. solanacearum with three lytic phages: ϕrsa1, ϕrsb1, and ϕrsl1. infection with ϕrsa1 and ϕrsb1, either alone or in combination with the other phages, resulted in rapid decrease of the host bacterial cell density. cells that were resistant to infection by these phages became evident approximately 30 h after phage addition to the culture. on the other hand, cells infe ... | 2011 | 21498752 |
| degradation of chlorobenzene by strain ralstonia pickettii l2 isolated from a biotrickling filter treating a chlorobenzene-contaminated gas stream. | a ralstonia pickettii species able to degrade chlorobenzene (cb) as the sole source of carbon and energy was isolated from a biotrickling filter used for the removal of cb from waste gases. this organism, strain l2, could degrade cb as high as 220 mg/l completely. following cb consumption, stoichiometric amounts of chloride were released, and co(2) production rate up to 80.2% proved that the loss of cb was mainly via mineralization and incorporation into cell material. the haldane modification o ... | 2011 | 21499764 |
| crystallization and preliminary x-ray diffraction studies of the catalytic domain of a novel chitinase, a member of gh family 23, from the moderately thermophilic bacterium ralstonia sp. a-471. | chitinase from the moderately thermophilic bacterium ralstonia sp. a-471 (ra-chic) is divided into two domains: a chitin-binding domain (residues 36-80) and a catalytic domain (residues 103-252). although the catalytic domain of ra-chic has homology to goose-type lysozyme, ra-chic does not show lysozyme activity but does show chitinase activity. the catalytic domain with part of an interdomain loop (ra-chic(89-252)) was crystallized under several different conditions using polyethylene glycol as ... | 2011 | 21505250 |
| engineering of polyhydroxyalkanoate (pha) synthase phac2(ps) of pseudomonas stutzeri via site-specific mutation for efficient production of pha copolymers. | the site-specific mutagenesis for pha synthase phac2(ps1317) from pseudomonas stutzeri 1317 was conducted for optimizing production of short-chain-length and medium-chain-length polyhydroxyalkanoates (scl-mcl pha). recombinant ralstonia eutropha phb-4 harboring double mutated phac2 ( ps1317 ) gene (phac2 ( ps ) qkst) produced 42 wt.% pha content in the cell dry weight (cdw) with 93 mol% 3-hydroxybutyrate (hb) as monomer in the pha copolymer. compared to that of wild-type phac2 ( ps1317 ), the hi ... | 2011 | 21509565 |
| functional domains and motifs of bacterial type iii effector proteins and their roles in infection. | a key feature of the virulence of many bacterial pathogens is the ability to deliver effector proteins into eukaryotic cells via a dedicated type three secretion system (t3ss). many bacterial pathogens, including species of chlamydia, xanthomonas, pseudomonas, ralstonia, shigella, salmonella, escherichia and yersinia, depend on the t3ss to cause disease. t3ss effectors constitute a large and diverse group of virulence proteins that mimic eukaryotic proteins in structure and function. a salient f ... | 2011 | 21517912 |
| establishment of an alternative phosphoketolase-dependent pathway for fructose catabolism in ralstonia eutropha h16. | the β-proteobacterium ralstonia eutropha h16 utilizes fructose and gluconate as carbon sources for heterotrophic growth exclusively via the entner-doudoroff pathway with its key enzyme 2-keto-3-deoxy-6-phosphogluconate (kdpg) aldolase. by deletion of the responsible gene eda, we constructed a kdpg aldolase-negative strain, which is disabled to supply pyruvate for energy metabolism from fructose or gluconate as sole carbon sources. to restore growth on fructose, an alternative pathway, similar to ... | 2011 | 21519932 |
| identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal rna gene sequence analysis. | clin microbiol infect 2011; 17: 776-779 abstract: dna sequence-based identification of pathogens from ocular samples of patients with clinically suspected eye infections was accomplished using 16s and internal transcribed spacer (its) ribosomal rna gene sequence analysis. pcr was positive for 24 of 99 samples tested. both culture and 16s rdna sequence analysis identified pseudomonas aeruginosa, streptococci and enterobacteriaceae. isolates misidentified as burkholderia cepacia by biochemical tes ... | 2011 | 21521414 |
| aerobic cometabolism of trichloroethene and cis-dichloroethene with benzene and chlorinated benzenes as growth substrates. | using inoculum from a microcosm study that exhibited aerobic transformation of cis-1,2-dichloroethene (cdce) and trichloroethene (tce) commensurate with biodegradation of monoaromatic compounds, enrichment cultures were developed by providing benzene, chlorobenzene (cb), dichlorobenzene (dcb) isomers and 1,2,4-trichlorobenzene as carbon and energy sources. isolates that grow on benzene, cb, 1,2-dcb and 1,3-dcb were identified as rhodococcus, ralstonia, variovorax and ralstonia spp., respectively ... | 2011 | 21531438 |
| classification of plant associated bacteria using rif, a computationally derived dna marker. | a dna marker that distinguishes plant associated bacteria at the species level and below was derived by comparing six sequenced genomes of xanthomonas, a genus that contains many important phytopathogens. this dna marker comprises a portion of the dnaa replication initiation factor (rif). unlike the rrna genes, dnaa is a single copy gene in the vast majority of sequenced bacterial genomes, and amplification of rif requires genus-specific primers. in silico analysis revealed that rif has equal or ... | 2011 | 21533033 |
| improved antibiotic resistance gene cassette for marker exchange mutagenesis in ralstonia solanacearum and burkholderia species. | marker exchange mutagenesis is a fundamental approach to understanding gene function at a molecular level in bacteria. new plasmids carrying a kanamycin resistance gene or a trimethoprim resistance gene were constructed to provide antibiotic resistance cassettes for marker exchange mutagenesis in ralstonia solanacearum and many antibiotic-resistant burkholderia spp. insertion sequences present in the flanking sequences of the antibiotic resistance cassette were removed to prevent aberrant gene r ... | 2011 | 21538255 |
| suppression of bacterial wilt and fusarium wilt by a burkholderia nodosa strain isolated from kalimantan soils, indonesia. | a trial was conducted to suppress bacterial wilt of tomato (bwt) caused by ralstonia solanacearum using biocontrol agents (bcas) isolated from soils in kalimantan, indonesia. five isolates were selected from 270 isolates as better performing bcas through screening four times using a pumice medium. the isolates selected were identified as burkholderia nodosa, burkholderia sacchari, burkholderia pyrrocinia and burkholderia terricola according to 16s rdna sequences, fatty acid composition and carbo ... | 2008 | 21558699 |
| effect of three organophosphorous nematicides on non-target nematodes and soil microbial community. | the toxicity of three organophosphorous nematicides, imicyafos, fosthiazate and cadusafos, to non-target organisms in soil was evaluated. imicyafos and fosthiazate had no significant inhibitory effect on the growth of fungal (fusarium oxysporum f. sp. lactucae, rhizoctonia solani and trichoderma viride) and bacterial (ralstonia solanacearum and pseudomonas fluorescens) strains in media at 12.5 to 200 mg l(-1). cadusafos, however, significantly inhibited the growth of all these strains except r. ... | 2008 | 21558727 |
| analyses of soluble and membrane proteomes of ralstonia eutropha h16 reveal major changes in the protein complement in adaptation to lithoautotrophy. | the soil-dwelling lithoautotrophic bacterium ralstonia eutropha h16 utilizes hydrogen as the key source of energy during aerobic growth on hydrogen and carbon dioxide. we examined the soluble and membrane protein complements of lithoautotrophically grown cells and compared them to the protein complements of cells grown organoheterotrophically on succinate. (14)n/(15)n-based inverse metabolic labeling in combination with gelc-ms led to the identification of 1452 proteins, 1174 of which could be q ... | 2011 | 21561103 |
| phylogenetic diversity and symbiotic effectiveness of root-nodulating bacteria associated with cowpea in the south-west area of japan. | the phylogenetic diversity of cowpea root-nodulating bacteria in the south-west of japan was investigated using 60 isolates. seeds of cowpea were aseptically sown in vermiculite and inoculated with a suspension of cowpea soil (cs) or bean soil (bs) or without a soil suspension as a control. cs and bs were collected from the kyushu university's farm (japan) at sites where cowpea and bean, respectively, have been cultivated previously. based on an analysis of the 16s rrna gene and the internal tra ... | 2009 | 21566362 |
| bacterial communities constructed in artificial consortia of bacteria and chlorella vulgaris. | we established artificial consortia of bacteria inoculated from soil and a green alga, chlorella vulgaris nies-227. the bacteria and the alga were mixed in a tube or partitioned in a dialysing culture vessel, and the bacterial composition was examined after cultivation. the community of bacteria formed in the consortia included those phylogenetically closely related to the genera phenylobacterium, brevundimonas, phyllobacterium, afipia, sphingomonas, sandaracinobacter, ramlibacter, ralstonia, ce ... | 2010 | 21576850 |
| mechanism of tomato bacterial wilt suppression in soil amended with lysine. | the effect of four amino acids, unutilizable by ralstonia solanacearum, on tomato bacterial wilt (tbw) was tested in three japanese soils. results confirmed our previous findings that the addition (2.5 mg g(-1) soil) of lysine and serine suppressed tbw, but that of tyrosine and valine did not. the number of the pathogen in non-rhizosphere soil, rhizosphere soil and the rhizoplane was markedly lower in the lysine and serine treatments than in the tyrosine and valine treatments, while the opposite ... | 2010 | 21576858 |
| control of tobacco mosaic virus by popw as a result of induced resistance in tobacco under greenhouse and field conditions. | in a previous study, we isolated a new harpin protein, popw, from the bacterium ralstonia solanacearum zj3721 that can induce a hypersensitive response (hr) in tobacco, nicotiana tabacum, leaves. in the current study, we demonstrate that, in a greenhouse experiment, popw induced tobacco-acquired resistance against the tobacco mosaic virus (tmv) with a biocontrol efficacy of 80.9% to 97.4% at a concentration as low as 25µg/ml in both popw-treated and neighboring leaves. the resistance induced by ... | 2011 | 21585268 |
| low temperature s(0) biomineralization at a supraglacial spring system in the canadian high arctic. | elemental sulfur (s(0) ) is deposited each summer onto surface ice at borup fiord pass on ellesmere island, canada, when high concentrations of aqueous h(2) s are discharged from a supraglacial spring system. 16s rrna gene clone libraries generated from sulfur deposits were dominated by β-proteobacteria, particularly ralstonia sp. sulfur-cycling micro-organisms such as thiomicrospira sp., and ε-proteobacteria such as sulfuricurvales and sulfurovumales spp. were also abundant. concurrent cultivat ... | 2011 | 21592302 |
| cloning and characterization of pr5 gene from curcuma amada and zingiber officinale in response to ralstonia solanacearum infection. | ginger (zingiber officinale roscoe), is an important spice crop that is badly affected by ralstonia solanacearum wilt. ginger does not set seed and sexual recombination has never been reported. in spite of extensive search in its habitats, no resistance source to ralstonia induced bacterial wilt, could be located in ginger. curcuma amada roxb. is a potential donor for bacterial wilt resistance to z. officinale, if the exact mechanism of resistance is understood. pathogenesis-related (pr)-5 prote ... | 2011 | 21594675 |
| expression of a wheat myb gene in transgenic tobacco enhances resistance to ralstonia solanacearum, and to drought and salt stresses. | myb transcription factors play diverse roles in plant growth, developmental processes and stress responses. a full-length cdna sequence of a myb gene, namely tapimp1, was isolated from wheat (triticum aestivum l.). the tapimp1 transcript level was significantly up-regulated by inoculation with a fungal pathogen bipolaris sorokiniana and by drought treatment. tapimp1 encodes the myb protein tapimp1 consisting of 323 amino acids. tapimp1 contains two myb dna binding domains (r2, r3), two putative ... | 2011 | 21597961 |
| [nife]- and [fes]-cofactors in the membrane-bound hydrogenase of ralstonia eutropha investigated by x-ray absorption spectroscopy: insights into o2-tolerant h2-cleavage. | molecular features that enable certain [nife] hydrogenases to catalyze hydrogen (h2) conversion in the presence of dioxygen (o2) were investigated in the present study. by means of x-ray absorption spectroscopy (xas) we compared the [nife] active site and fes clusters in the o2-tolerant membrane-bound hydrogenase (mbh) of ralstonia eutropha and the o2-sensitive periplasmic hydrogenase (ph) of desulfovibrio gigas. fe-xas indicated an unusual complement of iron-sulfur centers in the mbh, likely ba ... | 2011 | 21618994 |
| genomic analysis reveals versatile organisms for quorum quenching enzymes: acyl-homoserine lactone-acylase and -lactonase. | microbial virulence and their resistance to multiple drugs have obliged researchers to look for novel drug targets. virulence of pathogenic microbes is regulated by signal molecules such as acylated homoserine lactone (ahl) produced during a cell density dependent phenomenon of quorum sensing (qs). in contrast, certain microbes produce ahl-lactonases and -acylases to degrade qs signals, also termed as quorum quenching. mining sequenced genome databases has revealed organisms possessing conserved ... | 2011 | 21660112 |
| chemo-enzymatic synthesis of polyhydroxyalkanoate (pha) incorporating 2-hydroxybutyrate by wild-type class i pha synthase from ralstonia eutropha. | a previously established improved two-phase reaction system has been applied to analyze the substrate specificities and polymerization activities of polyhydroxyalkanoate (pha) synthases. we first analyzed the substrate specificity of propionate coenzyme a (coa) transferase and found that 2-hydroxybutyrate (2hb) was converted into its coa derivative. then, the synthesis of pha incorporating 2hb was achieved by a wild-type class i pha synthase from ralstonia eutropha. the pha synthase stereoselect ... | 2011 | 21667085 |
| identification and clarification of the role of key active site residues in bacterial glutathione s-transferase zeta/maleylpyruvate isomerase. | the maleylpyruvate isomerase nagl from ralstonia sp. strain u2, which has been structurally characterized previously, catalyzes the isomerization of maleylpyruvate to fumarylpyruvate. it belongs to the class zeta glutathione s-transferases (gstzs), part of the cytosolic gst family (cgsts). in this study, site-directed mutagenesis was conducted to probe the functions of 13 putative active site residues. steady-state kinetic information for mutants in the reduced glutathione (gsh) binding site, su ... | 2011 | 21672526 |
| production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by ralstonia eutropha in high cell density palm oil fermentations. | improved production costs will accelerate commercialization of polyhydroxyalkanoate (pha) polymer and pha-based products. plant oils are considered favorable feedstocks, due to their high carbon content and relatively low price compared to sugars and other refined carbon feedstocks. different pha production strategies were compared using a recombinant strain of ralstonia eutropha that produces high amounts of p(hb-co-hhx) when grown on plant oils. this r. eutropha strain was grown to high cell d ... | 2011 | 21809332 |
| oxyr is necessary for the hydrogen peroxide stress response and full virulence in ralstonia solanacearum. | the plant pathogen ralstonia solanacearum, which causes bacterial wilt disease, is exposed to reactive oxygen species (ros) during tomato infection and expresses diverse oxidative stress response (osr) genes during mid-stage disease on tomato. the r. solanacearum genome predicts that the bacterium produces multiple and redundant ros-scavenging enzymes, but only one known oxidative stress response regulator, oxyr. an r. solanacearum oxyr mutant had no detectable catalase activity, did not grow in ... | 2011 | 21803891 |
| monitoring the in situ crystallization of native biopolyester granules in ralstonia eutropha via infrared spectroscopy. | poly(3-hydroxybutyrate) (phb), a representative polyhydroxyalkanoate (pha), is a naturally occurring biopolyester stored as tiny, intracellular granules in microbial cells. in vivo, native phb granules are amorphous, stabilized by a monolayer membrane and intra-granule water. when subjected to varying environmental conditions, the native granules may become partially crystalline. the in situ crystallinity of native phb granules in ralstonia eutropha cells suspended in aqueous solution was monito ... | 2011 | 21801758 |
| isolation and characterization of a novel virulent podovirus which infects burkholderia pseudomallei. | abstract: burkholderia pseudomallei is a saprophytic soil bacterium and the etiological agent that causes melioidosis. it is naturally resistant to many antibiotics and therefore is difficult to treat. bacteriophages may provide an alternative source of treatment. we have isolated and characterised the bacteriophage phi bp-amp1. the phage is a member of the podoviridae family and has a genome size of ~ 45 kb. molecular data based on the gene which encodes for the phage tail tubular protein sugge ... | 2011 | 21791081 |
| prediction of protein-protein interactions between ralstonia solanacearum and arabidopsis thaliana. | ralstonia solanacearum is a devastating bacterial pathogen that has an unusually wide host range. r. solanacearum, together with arabidopsis thaliana, has become a model system for studying the molecular basis of plant-pathogen interactions. protein-protein interactions (ppis) play a critical role in the infection process, and some ppis can initiate a plant defense response. however, experimental investigations have rarely addressed such ppis. using two computational methods, the interolog and t ... | 2011 | 21786137 |
| surveillance of single-cell behavior in different subpopulations of ralstonia pickettii ar1 during growth and polyhydroxybutyrate production phases by flow cytometry. | most bacterial strains accumulate intracellular polyhydroxybutyrate (phb) granules as an energy reservoir, in response to fluctuations in their microenvironment. flow cytometry was applied for the analysis of single cells of ralstonia pickettii ar1 in response to changes in the culture conditions. two parameters, the phb production-related fl2 and side scatter (ssc) parameters, were used to monitor, distinguish and characterize different subpopulations in the growth and phb production phases. a ... | 2011 | 21780145 |
| sinorhizobium fredii usda257 and s. fredii usda191, cultivar-specific and non-specific symbionts of soybean, produce distinct cell surface appendages. | sinorhizobium fredii usda257 and s. fredii usda191 are fast-growing rhizobia that form nitrogen-fixing nodules on soybean roots. in contrast to usda191, usda257 exhibits cultivar specificity and can form nodules only on primitive soybean cultivars. in response to flavonoids released from soybean roots these two rhizobia secrete nodulation outer proteins (nop) to the extracellular milieu through a type iii secretion system. in spite of the fact that nop proteins are known to regulate legume nodul ... | 2011 | 21764962 |
| role of the hoxz subunit in the electron transfer pathway of the membrane-bound [nife]-hydrogenase from ralstonia eutropha immobilized on electrodes. | the role of the diheme cytochrome b (hoxz) subunit in the electron transfer pathway of the membrane-bound [nife]-hydrogenase (mbh) hetero-trimer from ralstonia eutropha h16 has been investigated. the mbh in its native hetero-trimeric state was immobilized on electrodes and subjected to spectroscopic and electrochemical analysis. surface enhanced resonance raman spectroscopy was used to monitor the redox and coordination state of the hoxz heme cofactors while concomitant protein film voltammetric ... | 2011 | 21761881 |
| biodegradation of tributyl phosphate by novel bacteria isolated from enrichment cultures. | tributyl phosphate (tbp) is an organophosphorous compound, used extensively (3000-5000 tonnes/annum) as a solvent for nuclear fuel processing and as a base stock in the formulation of fire-resistant aircraft hydraulic fluids and other applications. because of its wide applications and relative stability in the natural environment tbp poses the problem of pollution and health hazards. in the present study, fifteen potent bacterial strains capable of using tributyl phosphate (tbp) as sole carbon a ... | 2011 | 21755325 |
| a non-nadb type l-aspartate dehydrogenase from ralstonia eutropha strain jmp134: molecular characterization and physiological functions. | we report the molecular characterization and physiological function of a novel l-aspartate dehydrogenase (aspdh). the purified enzyme was a 28-kda dimeric protein, exhibiting high catalytic activity for l-aspartate (l-asp) oxidation using nad and/or nadp as cofactors. quantitative real-time pcr analysis indicated that the genes involved in the aspdh gene cluster, poly-3-hydroxyalkanoate (pha) biosynthesis, and the tca cycle were substantially induced by l-asp in wild-type cells. in contrast, exp ... | 2011 | 21821928 |
| diversity analysis of diazotrophic bacteria associated with the roots of tea (camellia sinensis (l.) o. kuntze). | the diversity elucidation by amplified ribosomal dna restriction analysis and 16s rdna sequencing of 96 associative diazotrophs, isolated from the feeder roots of tea on enriched nitrogen-free semisolid media, revealed the predominance of gram-positive over gram-negative bacteria within the kangra valley in himachal pradesh, india. the gram-positive bacteria observed belong to two taxonomic groupings; firmicutes, including the genera bacillus and paenibacillus; and actinobacteria, represented by ... | 2011 | 21715960 |
| complete genome sequence of the type strain cupriavidus necator n-1. | here we announce the complete genome sequence of the copper-resistant bacterium cupriavidus necator n-1, the type strain of the genus cupriavidus. the genome consists of two chromosomes and two circular plasmids. based on genome comparison, the chromosomes of c. necator n-1 share a high degree of similarity with the two chromosomal replicons of the bioplastic-producing hydrogen bacterium ralstonia eutropha h16. the two strains differ in their plasmids and the presence of hydrogenase genes, which ... | 2011 | 21742890 |
| biosynthesis of a complex yersiniabactin-like natural product via the mic locus in phytopathogen ralstonia solanacearum. | a genome mining study in the plant pathogenic bacterium ralstonia solanacearum gmi1000 unveiled a polyketide synthase/nonribosomal peptide synthetase gene cluster putatively involved in siderophore biosynthesis. insertional mutagenesis confirmed the respective locus to be operational under iron-deficient conditions and spurred the isolation of the associated natural product. bioinformatic analyses of the gene cluster facilitated the structural characterization of this compound, which was subsequ ... | 2011 | 21724891 |
| genome-scale reconstruction and in silico analysis of the ralstonia eutropha h16 for polyhydroxyalkanoate synthesis, lithoautotrophic growth, and 2-methyl citric acid production. | abstract: | 2011 | 21711532 |
| lobar pneumonia caused by ralstonia pickettii in a sixty-five-year-old han chinese man: a case report. | abstract: | 2011 | 21843320 |
| insights into the structure of the active site of the o(2)-tolerant membrane bound [nife] hydrogenase of r. eutropha h16 by molecular modelling. | structural models for the ni-b state of the wild-type and c81s protein variant of the membrane-bound [nife] hydrogenase from ralstonia eutropha h16 were derived by applying the homology model technique combined with molecular simulations and a hybrid quantum mechanical/molecular mechanical approach. the active site structure was assessed by comparing calculated and experimental ir spectra, confirming the view that the active site structure is very similar to those of anaerobic standard hydrogena ... | 2011 | 21833416 |
| the yopj superfamily in plant-associated bacteria. | bacterial pathogens employ the type iii secretion system to secrete and translocate effector proteins into their hosts. the primary function of these effector proteins is believed to be the suppression of host defence responses or innate immunity. however, some effector proteins may be recognized by the host and consequently trigger a targeted immune response. the yopj/hopz/avrrxv family of bacterial effector proteins is a widely distributed and evolutionarily diverse family, found in both anima ... | 2011 | 21726386 |
| biodegradation kinetics of 4-fluorocinnamic acid by a consortium of arthrobacter and ralstonia strains. | arthrobacter sp. strain g1 is able to grow on 4-fluorocinnamic acid (4-fca) as sole carbon source. the organism converts 4-fca into 4-fluorobenzoic acid (4-fba) and utilizes the two-carbon side-chain for growth with some formation of 4-fluoroacetophenone as a dead-end side product. we also have isolated ralstonia sp. strain h1, an organism that degrades 4-fba. a consortium of strains g1 and h1 degraded 4-fca with monod kinetics during growth in batch and continuous cultures. specific growth rate ... | 2011 | 21728015 |
| polyhydroxyalkanoate (pha) synthesis by class iv pha synthases employing ralstonia eutropha phb(-)4 as host strain. | class iv polyhydroxyalkanoate (pha) synthase from bacillus cereus yb-4 (pharc(yb4)) or b. megaterium nbrc15308(t) (pharc(bm)) was expressed in ralstonia eutropha phb(-)4 to compare the ability to produce pha and the substrate specificity of pharcs. pharc(yb4) produced significant amounts of pha and had broader substrate specificity than pharc(bm). | 2011 | 21821924 |
| exploitation of host polyubiquitination machinery through molecular mimicry by eukaryotic-like bacterial f-box effectors. | microbial pathogens have evolved exquisite mechanisms to interfere and intercept host biological processes, often through molecular mimicry of specific host proteins. ubiquitination is a highly conserved eukaryotic post-translational modification essential in determining protein fate, and is often hijacked by pathogenic bacteria. the conserved skp1/cul1/f-box (scf) e3 ubiquitin ligase complex plays a key role in ubiquitination of proteins in eukaryotic cells. the f-box protein component of the s ... | 2010 | 21687758 |
| complete genome sequence of the plant pathogen ralstonia solanacearum strain po82. | ralstonia solanacearum strain po82, a phylotype iib/sequevar 4 strain, was found to be pathogenic to both solanaceous plants and banana. here, we report the complete genome sequence of po82 and its comparison with seven published r. solanacearum genomes. | 2011 | 21685279 |
| priming by rhizobacterium protects tomato plants from biotrophic and necrotrophic pathogen infections through multiple defense mechanisms. | a selected strain of rhizobacterium, pseudomonas putida strain lsw17s (lsw17s), protects tomato plants (lycopersicon esculentum l. cv. seokwang) from bacterial speck by biotrophic pseudomonas syringae pv. tomato strain dc3000 (dc3000) and bacterial wilt by necrotrophic ralstonia solanacearum kacc 10703 (rs10703). to investigate defense mechanisms induced by lsw17s in tomato plants, transcription patterns of pathogenesis-related (pr) genes and h(2)o(2) production were analyzed in plants treated w ... | 2011 | 21710203 |
| plant ferredoxin-like protein (pflp) outside chloroplast in arabidopsis enhances disease resistance against bacterial pathogens. | protection of crops against bacterial disease is an important issue in agricultural production. one of the strategies to lead plants become resistant against bacterial pathogens is employing a transgene, like plant ferredoxin-like protein (pflp). pflp is a photosynthetic type ferredoxin isolated from sweet pepper and contains a signal peptide for targeting towards chloroplasts. our previous reports indicated that transgenic plants with this protein are more resistant against bacterial pathogens. ... | 2010 | 21802603 |
| cell growth and p(3hb) accumulation from co(2) of a carbon monoxide-tolerant hydrogen-oxidizing bacterium, ideonella sp. o-1. | cell growth and accumulation of polyhydroxybutyric acid, p(3hb), from co(2) in autotrophic condition of a newly isolated hydrogen-oxidizing bacterium, the strain o-1, was investigated. the bacterium, which was deposited in the japan collection of microorganisms as jcm17105, autotrophically grows by assimilating h(2), o(2), and co(2) as substrate. 16s rrna gene sequence of the bacterium was the closest to ideonella dechloratans (99%). specific growth rate of the strain o-1 was faster than a hydro ... | 2011 | 21695533 |
| biosynthesis of polyhydroxyalkanoates containing 2-hydroxybutyrate from unrelated carbon source by metabolically engineered escherichia coli. | we have previously reported in vivo biosynthesis of polylactic acid (pla) and poly(3-hydroxybutyrate-co-lactate) [p(3hb-co-la)] employing metabolically engineered escherichia coli strains by the introduction of evolved clostridium propionicum propionyl-coa transferase (pct( cp )) and pseudomonas sp. mbel 6-19 polyhydroxyalkanoate (pha) synthase 1 (phac1( ps6-19)). using this in vivo pla biosynthesis system, we presently report the biosynthesis of phas containing 2-hydroxybutyrate (2hb) monomer b ... | 2011 | 21842437 |