| [severe transient neutropenia due to parvovirus b19]. | a 10-year-old girl was admitted with a 3-day history of fever, cough, abdominal pain and vomiting. severe neutropenia (total neutrophil count 186/mm3), a mild increase in alt and ast, and a positive titer of igm antibodies against parvovirus b19 were found. the neutropenia resolved and liver enzymes became normal as she recovered. we conclude that parvovirus b19 infection should be considered in the evaluation of an acute illness accompanied by severe neutropenia. | 1999 | 10959297 |
| rheumatoid arthritis: a viral aetiology? | | 2000 | 10962648 |
| [parvovirus b19 infection in hydrops fetalis]. | in a retrospective study from 210 foetal autopsies carried out in a period between 1992 and 1999 fifteen hydropic foetuses were found. the cause of the hydrops was shown to be rh incompatibility in one case only. the cause of hydrops was not discernible in one case. in the others pathological examination clarified the cause and pathomechanism of non-immune hydrops. one isolated cystic hygroma, one monochorionic twin pregnancy with twin to twin transfusion, one case of sacrococcygeal teratoma and ... | 2000 | 10962903 |
| identification of a novel rna splicing pattern as a basis of restricted cell tropism of erythrovirus b19. | prior studies on the transcription of erythrovirus b19 have identified a short leader sequence associated with all spliced viral transcripts. while some variability has been observed in the acceptor for this first intron, studies to date in both permissive and nonpermissive cell types have reported a unique splice donor site. in the semipermissive mb-02 cell line, we have found that splicing of this first intron proceeds almost exclusively via a cryptic ct donor downstream of the previously repo ... | 2000 | 10964772 |
| [seroprevalence of parvovirus b19 in our area and its distribution by ages and sexes]. | | 2000 | 10974772 |
| pathogenesis of human parvovirus b19 in rheumatic disease. | | 2000 | 10976079 |
| parvovirus b19-related acute hepatitis in an immunosuppressed kidney transplant. | | 2000 | 10978424 |
| acute parvovirus b19 infection as a cause of autoimmune hemolytic anemia. | | 2000 | 10980646 |
| the age-specific prevalence of human parvovirus immunity in victoria, australia compared with other parts of the world. | the age-specific immunity to human parvovirus infection was estimated in victoria, australia using prospectively collected samples from the royal children's hospital, the royal women's hospital and the australian red cross blood service and from sera stored at the victorian infectious diseases reference laboratory (vidrl). all testing was performed at vidrl using a commercial enzyme-linked immunosorbent assay (biotrin). of the 824 sera tested, 28% of those drawn from people aged 0-9 years contai ... | 2000 | 10982069 |
| dermatomyositis associated with the presence of parvovirus b19 dna in muscle. | we report a case of dermatomyositis associated with molecular evidence of parvovirus b19 dna in two muscle biopsies collected 5 months apart. igg- but not igm-specific antibodies were detected in serum. none of four serum samples was positive for parvovirus b19 dna. the two biopsies contained b19 vp1 sequences and the second one was also positive for ns1. this is the first report of viral parvovirus b19 dna in muscle of a patient with dermatomyositis. latent muscle infection may contribute to th ... | 2000 | 10986312 |
| quebec's safe working conditions for a safe maternity experience program: survey of consultant physicians and human parvovirus b19 in montreal-centre. | | 2000 | 10986781 |
| viral safety of solvent/detergent-treated plasma. | | 2000 | 10988322 |
| parvovirus b19 infection reminiscent of myelodysplastic syndrome in three children with chronic hemolytic anemia. | the authors have seen transient pancytopenia with erythroid hypoplasia and striking trilineage myelodysplasia reminiscent of true myelodysplastic syndrome (mds) in 3 children, 1 with thalassemia intermedia and the other 2 with previously undiagnosed hereditary spherocytosis. in these 3 children transient pancytopenia and myelodysplasia coincided with serological evidence of acute parvovirus-b19 (pv-b19) infection, strongly suggesting their relevance. it is of interest that these 3 cases were enc ... | 2000 | 10989468 |
| [presence of anticardiolipin antibodies in a patient with polyarthritis caused by parvovirus b19]. | | 2000 | 10996879 |
| [a case of guillain-barré syndrome (gbs) following human parvovirus b19 infection]. | human parvovirus b19 (hpv-b19) infection is recently known to produce variable clinical manifestations, but neurological disorder associated with this infection is uncommon. we reported a case of gbs associated with transient lupus-like status following hpv-b19 infection. this is the first report describing an adult case of gbs following hpv-b19 infection. a healthy female, aged 33, developed erythema infectiosum simultaneously with her 5-year-old daughter. on the same day, she noticed leg fatig ... | 2000 | 11002730 |
| endocapillary proliferative glomerulonephritis in a patient with parvovirus b19 infection. | a 45-year-old woman developed acute nephritic syndrome after erythema infectiosum. laboratory data on admission showed decreased serum c3, c4, and ch50 levels and the presence of both immunoglobulin (ig) m and igg antibodies to human parvovirus b19 (hpv). a renal biopsy showed diffuse endocapillary proliferative glomerulonephritis. immunofluorescence microscopy indicated 2+ granular staining for igg, igm, and c3 over the mesangial area and along glomerular capillary walls. hpv antigen was also d ... | 2000 | 11007690 |
| removal of parvovirus b19 from hemoglobin solution by nanofiltration. | parvovirus b19 (b19), which may contaminate red cell components for blood transfusion, is known to be resistant to several viral inactivation methods. to increase the safety of hemoglobin solutions as a source of red cell substitutes, we investigated the removal of parvovirus b19 from hemoglobin solution using nanofiltration. the hemoglobin solution spiked with parvovirus b19 was tangentially filtered using the bmm-35 filter (mean pore size of 35 nm) followed by bmm-15. the parvovirus b19 of 10( ... | 2000 | 11009110 |
| digital arterial occlusive disease. | | 2000 | 11009166 |
| neutropenia accompanying parvovirus b19 infection after gynecologic surgery. | the hematologic data and symptoms of 7 patients seropositive for parvovirus b19 igm antibody after gynecologic surgery were analysed. parvovirus may have been transmitted by fibrin glue prepared from heat-treated human plasma and used for hemostasis during surgery. the peripheral blood neutrophil count decreased to below 1 x 10(9)/l between postoperative day (pod) 10 and 18, but recovered spontaneously to within the normal range. g-csf injection was effective in preventing neutropenia or obtaini ... | 2000 | 11014891 |
| [leukocytopenia and thrombocytopenia preceded by human parvovirus b19 infection: report of three adult cases]. | in saiki city, oita, japan, erythema infectiosum in children has been prevalent from june, 1999 to the time of writing (january, 2000). we present three adult cases of parvovirus b19-associated leukocytopenia and thrombocytopenia that developed during this epidemic. between june and november, 1999, a 32-year-old woman, a 38-year-old woman, and a 63-year-old man were referred to our hospital for treatment of leukocytopenia and thrombocytopenia. all complained of common cold-like symptoms. their w ... | 2000 | 11020984 |
| passive immunity in prevention and treatment of infectious diseases. | antibodies have been used for over a century in the prevention and treatment of infectious disease. they are used most commonly for the prevention of measles, hepatitis a, hepatitis b, tetanus, varicella, rabies, and vaccinia. although their use in the treatment of bacterial infection has largely been supplanted by antibiotics, antibodies remain a critical component of the treatment of diptheria, tetanus, and botulism. high-dose intravenous immunoglobulin can be used to treat certain viral infec ... | 2000 | 11023960 |
| impaired gamma interferon responses against parvovirus b19 by recently infected children. | parvovirus b19 is the causative agent of "fifth disease" of childhood. it has been implicated in a variety of conditions, including unsuccessful pregnancy and rheumatoid arthritis, and is a potential contaminant of blood products. there has been little study of immunity to parvovirus b19, and the exact nature of the protective humoral and cell-mediated immune response is unclear. immune responses to purified virus capsid proteins, vp1 and vp2, were examined from a cohort of recently infected chi ... | 2000 | 11024117 |
| parvovirus b19 infection: association with third-trimester intrauterine fetal death. | | 2000 | 11028594 |
| chronic parvovirus b-19 meningoencephalitis with additional detection of epstein-barr virus dna in the cerebrospinal fluid of an immunocompetent patient. | parvovirus b19 dna was detected by polymerase chain reaction in the brain biopsy specimen from a 67-year-old immunocompetent woman with severe chronic lymphocytic meningoencephalitis. in addition to parvovirus b19, epstein-barr virus dna was identified in the csf. genomic material from epstein-barr virus was absent in the brain tissue. clinical symptoms and csf pleocytosis improved under long-term corticosteroid-treatment. the aetio-pathogenetic role of parvovirus b19 and the possible meaning of ... | 2000 | 11031695 |
| parvovirus b19 infection associated with the production of anti-neutrophil cytoplasmic antibody (anca) and anticardiolipin antibody (acl). | we described four patients who had clinical diagnosis of erythema infectiosum and presented with skin rash, polyarthralgia, polyarthritis, and mild fever. anti-parvovirus b19 igm and igg antibodies were found in all four patients and parvovirus b19 dna was detected in three of the four patients by polymerase chain reaction (pcr) in sera using standard methods. anticardiolipin antibody (acl) was positive in three of the four patients included three with anti-beta2 glycoprotein i (beta2gpi). the i ... | 2000 | 11035424 |
| parvovirus arthropathy outbreak in southwestern united states. | we describe an outbreak of parvovirus (pv) arthropathy that was detected in a rheumatology clinic in san antonio, texas, during the winter of 1994. parvovirus b19 causes acute symmetric polyarthritis (aspa) in adults. in the us, the majority of cases described are from the northern us. | 2000 | 11036842 |
| low-dosage immunoglobulins for an infant with hypogammaglobulinemia, maple syrup urine disease, and parvovirus b19-associated aplastic crisis. | | 2000 | 11037872 |
| [parvovirus b19 antibodies in the blood in 17-34-year-old women]. | in order to assess the proportion of women in fertile age who lack antibodies against parvovirus b19 the authors assessed in 2 groups of sera (63 and 40 sera) taken from 17-34-year-old women antibodies against this virus by the elisa method (seiken). virus-specific antibodies, class igg were detected only in 67% of the examined subjects which indicates that in one third of the women there is a risk of transplacental infection of the foetus in case of infection with the parvovirus b19 during preg ... | 1999 | 11038670 |
| early onset of hemophagocytic syndrome following allogeneic bone marrow transplantation. | we report a 40-year-old man who presented with acute onset of hemophagocytic syndrome (hps) after allogeneic bone marrow transplantation (allobmt) for acute myelogenous leukemia. on day 8 after allobmt, the patient suddenly manifested high-grade fever, transfusion-resistant severe anemia, and thrombocytopenia. neither veno-occlusive disease nor thrombotic microangiopathy was documented. the level of ferritin in serum was elevated to 1192 ng/ml. a bone marrow aspiration test on day 16 showed a ma ... | 2000 | 11039676 |
| papular-purpuric gloves and socks syndrome in hiv-positive patients. | three hiv-positive women showed clinical signs of papular-purpuric gloves and socks syndrome and serologic evidence of acute parvovirus b19 infection. the course of the disease was complicated by anemia and persistent skin lesions, probably related to inadequate immune response. because anemia in aids patients may be due to many causes, the history of recent parvovirus b19 infection is helpful in suggesting the etiologic diagnosis. | 2000 | 11044821 |
| parvovirus b19 in idiopathic thrombocytopenic purpura. | | 2000 | 11054052 |
| clinical illness due to parvovirus b19 infection after infusion of solvent/detergent-treated pooled plasma. | lipid-enveloped viruses such as hiv, hbv, and hcv can be inactivated by treatment with solvents and detergents. hav and human parvovirus b19 lack lipid envelopes and are not inactivated. solvent/detergent-treated pooled plasma (s/d plasma) contains neutralizing antibodies, but it is not known whether the parvovirus b19 antibody content is sufficient to prevent transmission of the disease. a patient is described who developed a clinical illness due to parvovirus b19 infection after the infusion o ... | 2000 | 11061856 |
| seroprevalence of cytomegalovirus, toxoplasma and parvovirus in pregnancy. | the aim of our study was to determine the seroprevalence of cytomegalovirus (cmv), toxoplasma and parvovirus infection in our local antenatal population, and to see the effects, if any, of age, race, parity and nationality on its seroprevalence. | 2000 | 11063178 |
| detection of parvovirus b19 igm by antibody capture enzyme immunoassay: receiver operating characteristic analysis. | parvovirus b19 infection can cause severe effects in high-risk groups including pregnant women and immunocompromised individuals. although serological detection of b19 infection is commonplace, minimal information is available on the absolute performance characteristics of various tests for the detection of b19 igm. the performance of the first parvovirus b19 igm enzyme immunoassay to be cleared by the us food and drug administration (fda) is described. the immunoassay cut-off has been establish ... | 2000 | 11064115 |
| aplastic crisis caused by parvovirus b19 in an adult patient with sickle-cell disease. | we describe a case of aplastic crisis caused by parvovirus b19 in an adult sickle-cell patient presenting with paleness, tiredness, fainting and dyspnea. the absence of reticulocytes lead to the diagnosis. anti-b19 igm and igg were detected. reticulocytopenia in patients with hereditary hemolytic anemia suggests b19 infection. | 2000 | 11064584 |
| [autoimmune thrombocytopenia and erythroid hypoplasia associated with hepatitis a]. | a 53-year-old woman developed fever and fatigue after eating raw oysters, and consulted a local clinic. she exhibited liver dysfunction and jaundice, and was therefore referred and admitted to our hospital. hepatitis a was diagnosed based on virus markers, and palliative therapy was administered. after admission, the increased levels of transaminases and lactic acid dehydrogenase (ldh) normalized rapidly. however, marked thrombocytopenia was observed, and steroid pulse therapy was administered. ... | 2000 | 11070936 |
| severe digital arterial occlusive disease and acute parvovirus b19 infection. | we report a case of arterial occlusive disease and digital ischaemia associated with acute parvovirus b19 infection. treatment with intravenous epoprostanol improved her symptoms. | 2000 | 11071192 |
| acute parvovirus b19 infection mimicking juvenile myelomonocytic leukemia. | an 11-month-old patient with parvovirus infection mimicking juvenile myelomonocytic leukemia (jmml) is presented. the patient's history, presenting physical and laboratory features, was suggestive of jmml and consisted of fever, hepatosplenomegaly, lymphadenopathy, desquamation of the skin, anemia, leukocytosis with monocytosis and trilineage dysplastic findings of the peripheral blood and bone marrow. however, positive igm titers for parvovirus b19 followed by seroconversion, negative cytogenet ... | 2000 | 11073169 |
| tyramide signal amplification of biotinylated probe in dot-blot hybridization assay for the detection of parvovirus b19 dna in serum samples. | highly sensitive assay systems are necessary for large-scale virological screenings. we evaluated the use of tyramide signal amplification (tsa) for biotinylated probe in dot-blot hybridization assay to detect b19 dna in serum samples. the probe was constructed by pcr and directly labeled with biotin during amplification reaction. the sensitivity of the dot-blot hybridization assay with tsa detection method was evaluated in comparison with a hybridization assay using the direct detection of biot ... | 2000 | 11074066 |
| viral safety of haemophilia treatment products. | the epidemics of hiv and hepatitis c in treated haemophiliacs spurred rapid technological advances in the viral safety of clotting factor concentrates produced from large donor pools. sequential steps are now employed to minimize infectious risks. the initial viral burden is reduced by screening donors and by testing individual donations and plasma pools for antivirus antibodies, viral antigens, and nucleic acid. these techniques are supplemented by nonspecific viral reduction steps based on phy ... | 2000 | 11087169 |
| infection with parvovirus b19. | human parvovirus b19 is common and widespread. major manifestations of b19 infection are transient aplastic crisis, erythema infectiosum, hydrops fetalis, acute and chronic rheumatoid-like arthropathy and, in the immunocompromised host, chronic or recurrent bone marrow infection. less common presentations include skin eruptions, isolated cytopenias, vasculitis, hepatitis, and neuropathies. increasing awareness of the clinical manifestations of b19 infection makes parvovirus b19 an emerging virus ... | 1999 | 11095799 |
| survey of parvovirus b19 infection in a cohort of pregnant women in belém, brazil. | in our study, 300 pregnant women were screened for the presence of human parvovirus b19 igg and igm antibodies by an enzyme-linked immunosorbent assay (elisa). overall, 253 (84.3%) were found to be igg-positive and igm-negative (igg+gm ), 42 (14%) had neither igg nor igm antibodies (igg /igm ) and 5 (1.7%) were both igm- and igg-positive (igg+/igm+). maternal serology was performed routinely for cytomegalovirus, rubella, toxoplasmosis and syphilis. all igg /igm and igg+/igm+ women were followed ... | 1999 | 11098187 |
| a causal role for parvovirus b19 infection in adult dermatomyositis and other autoimmune syndromes. | infection with parvovirus b19 (b19) has been associated with connective tissue disease (ctd) stigmata, namely, a systemic lupus erythematosus (sle)-like illness, seronegative polyarthritis resembling rheumatoid arthritis, and vasculitis. the dermatopathology and pathogenetic basis of such b19-associated ctd-like syndromes have not been elucidated. | 2000 | 11100810 |
| renal involvement of human parvovirus b19 in an immunocompetent host. | human parvovirus b19, which is most commonly known to cause erythema infectiosum in children, is also known to cause infection in adults, with complications ranging from a self-limited polyarthropathy in immunocompetent patients to hydrops fetalis in pregnant women, transient aplastic crises in patients with chronic hemolytic anemias, and chronic aplastic anemia in immunocompromised hosts. we describe a previously healthy immunocompetent woman who presented with manifestations of acute parvoviru ... | 2001 | 11106317 |
| [genetic diversity of viruses. consequences for screening and prevention]. | the evolution of viruses contributes to their diversification, whether it be a result of their own replication, or host-pressure dependent. certain viral types, groups or subtypes are therefore found in certain regions of the world or in certain populations. the development of blood screening reagents is nearly always based on viral antigens or viral sequences derived from 'prototype' strains or antibodies raised against these prototype strains. therefore in situations where an individual is inf ... | 2000 | 11109632 |
| fatal intrauterine adenoviral endomyocarditis with aortic and pulmonary valve stenosis: diagnosis by polymerase chain reaction. | we report a case of fatal hydrops fetalis owing to adenoviral endomyocarditis with aortic and pulmonary valve stenosis. a 1850-g macerated male stillborn delivered 1 week after fetal ultrasonography showed hydrops, cardiomegaly, and possible aortic valve stenosis. autopsy confirmed hydrops and showed thickened, fibrotic semilunar valves with stenosis. the myocardium was focally fibrotic with areas of calcification. polymerase chain reaction study of myocardial and aortic valve tissue was positiv ... | 2000 | 11112222 |
| erythroblastopenia and parvovirus b19 infection in a healthy child. | | 2000 | 11114822 |
| mapping of b-cell epitopes on human parvovirus b19 non-structural and structural proteins. | in the present study, the immune reactivity to parvovirus b19 (b19) proteins and variations in antigenic reactivity in different clinical manifestations were investigated. sera from healthy b19 igg positive individuals were evaluated for antibody reactivity against linear peptides. three antigenic regions (amino acid number 191-206, 271-286, 371-386) on the b19 non-structural (ns) protein 1 were identified. the highest seroreactivity against these peptides was found against amino acid number 271 ... | 2000 | 11115697 |
| different patterns of restriction to b19 parvovirus replication in human blast cell lines. | b19 parvovirus can replicate in erythroid progenitor cells and in a small number of human blast cell lines. to better understand and analyze the b19 virus replicative cycle, we performed and compared the infection of bone marrow cells and of different blast cell lines with erythroblastoid and megakaryoblastoid phenotypic characteristics (ut-7, tf-1, m-07, and b1647). following in vitro infection, b19-specific nucleic acids were characterized with regard to the genome-replicative intermediates, t ... | 2000 | 11118359 |
| direct ex vivo measurement of cd8(+) t-lymphocyte responses to human parvovirus b19. | parvovirus b19 is a common human pathogen which can cause severe syndromes, including aplastic anemia and fetal hydrops. the mapping of the first parvovirus b19-derived cd8(+) t-lymphocyte epitope is described. this hla-b35-restricted peptide derives from the nonstructural (ns1) protein and is strongly immunogenic in b19 virus-seropositive donors. | 2001 | 11119624 |
| pure red cell aplasia due to parvovirus b19 infection in a renal transplant patient: a case report. | | 2000 | 11120017 |
| discontinuation of immunosuppressive antimetabolite for parvovirus b19-associated anemia in kidney transplant patients. | | 2000 | 11120023 |
| parvovirus b19 transmitted by bone marrow. | we describe a case of symptomatic parvovirus b19 infection transmitted by bone marrow (bm). the infection caused prolonged anaemia, thrombocytopenia, arthralgia and erythema infectiosum in a 16-year-old girl with acute myeloid leukaemia receiving a bm transplant (bmt). the bm donor was a 19-year-old asymptomatic brother who had parvovirus b19 viraemia at the time of bm harvest. sequencing of the vp2 gene from the patient and the donor showed a perfect match of dna sequences, confirming the mode ... | 2000 | 11122118 |
| the vp1-unique region of parvovirus b19: amino acid variability and antigenic stability. | the unique region of structural protein vp1 of parvovirus b19 (erythrovirus b19) is important for eliciting neutralizing antibodies that are responsible for eliminating the virus from the peripheral blood and for inducing lifelong immunity. neutralizing human mabs bind a conformationally defined epitope spanning vp1 residues 30-42. the dna sequence encoding the vp1-unique region was determined in parvovirus b19 isolated from peripheral blood and amniotic fluid of nine acutely infected pregnant w ... | 2001 | 11125172 |
| congenital parvovirus b19 infection: experience of a recent epidemic. | we report the management and outcome of 6 cases of non-immune fetal hydrops secondary to parvovirus b19 infection presenting over a 5-month period. | 2001 | 11125246 |
| recombinant parvovirus b19 empty capsids inhibit fetal hematopoietic colony formation in vitro. | erythroid lineage cells are target cells for human parvovirus b19, and a natural infection often results in transient anemia. to determine whether recombinant b19 capsid proteins (vp1/vp2) also inhibit human hematopoietic progenitor growth, a model system was set up. the b19 capsids were inoculated into primary cultures of hematopoietic stem cells derived from human fetal liver, resulting in a 70-95% reduction of bfu-e (burst-forming unit erythroid cells) as compared with the medium control. a s ... | 2001 | 11125248 |
| pure red cell aplasia caused by parvovirus b19 infection in solid organ transplant recipients: a case report and review of literature. | human parvovirus b19 (pv b19) is one of the several recently described 'emerging viruses' and has been identified as the etiological agent of 'fifth disease' in childhood. human pv b19, which is the etiological agent of transient erythroblastopenia in hemolytic anemia, is also a recognized rare cause of red cell aplasia in immunocompromised patients, including transplant recipients. to date, 26 cases of pv b19-induced red cell aplasia have been reported in solid organ transplant recipients. twel ... | 2000 | 11127313 |
| prevalence of antibodies to parvovirus b 19 in thailand. | infection with human parvovirus b 19, a single-stranded non-enveloped dna virus of worldwide distribution, is rather common and displays a broad spectrum of clinical manifestations of varying severity, depending on the patient's immune response. as the target of infection are the erythroid precursor cells, patients can experience an aplastic crisis. usually, at least in immunocompetent individuals, viremia ceases with the appearance of virus-specific antibodies in the patient's serum whereupon t ... | 2000 | 11127352 |
| outbreaks of human-herpes virus 6 (hhv-6) infection in day-care centers in belém, pará, brazil. | a total of 730 children aged less than 7 years, attending 8 day-care centers (dccs) in belém, brazil were followed-up from january to december 1997 to investigate the occurrence of human-herpes virus 6 (hhv-6) infection in these institutional settings. between october and december 1997 there have been outbreaks of a febrile- and -exanthematous disease, affecting at least 15-20% of children in each of the dccs. both serum- and- plasma samples were obtained from 401 (55%) of the 730 participating ... | 2000 | 11136516 |
| congenital anemia caused by parvovirus b19 infection. | | 2000 | 11144391 |
| bone marrow failure in children with acute liver failure. | aplastic anemia is a rare but well-recognized complication of acute hepatitis and acute liver failure. the cause is unknown, and the condition is fatal without bone marrow recovery. treatment includes immunosuppression regimens or bone marrow transplantation. the purpose of this study was to investigate the incidence, cause, treatment, and outcomes of this disorder in children. | 2000 | 11144443 |
| parovirus b19-associated mono- and oligoarticular arthritis may evolve into a chronic inflammatory arthropathy fulfilling criteria for rheumatoid arthritis or spondylarthropathy. | | 2000 | 11147771 |
| quantitative evidence for persistence of human parvovirus b19 dna in an immunocompetent individual. | | 2000 | 11152317 |
| [a case of systemic lupus erythematosus with pure red cell aplasia possibly caused by persistent infection of human parvovirus b 19]. | we reported a 68-old female who was diagnosed to have systemic lupus erythematosus 18 years ago. she had been well under 5 mg of prednisolone until 1995, when she felt severe shortness of breath. laboratory examinations disclosed severe anemia accompanying mild thrombocytopenia. bone marrow aspiration revealed a complete absence of erythroid progenitor cells. she was diagnosed to have pure red cell aplasia (prca) as well as antiphospholipid syndrome. a judicious use of methylprednisolone includi ... | 1998 | 11155595 |
| parvovirus b19: implications for transfusion medicine. summary of a workshop. | this 1-day workshop showed that the infectivity of b19 dna in donor blood and the neutralizing action of different antibodies present in the donated blood are not yet fully understood. it is possible that b19-induced anemia and reticulocytopenia are not being recognized in transfused recipients other than those in specific risk groups. the testing of blood components for any infectious agent is usually clinically driven, and, if b19 nat were recommended at the present time in other than plasma p ... | 2001 | 11161258 |
| validation and standardisation of nucleic acid amplification technology (nat) assays for the detection of viral contamination of blood and blood products. | standardisation of nat assays is necessary before the introduction of such assays for routine screening of blood and blood products for viral contaminants such as hbv, hcv, and hiv-1. standardisation can be achieved by the use of well-characterised reference materials (working reagents) to validate each assay run. working reagents for hcv, hiv-1, hbv, hav, and human parvovirus b19 have been established by the nibsc. such reagents and reference panels are also available from other official medici ... | 2001 | 11163577 |
| chronic autoimmune thrombopenia/neutropenia in a boy with persistent parvovirus b19 infection. | we report an 11-year-old boy presenting with splenomegaly, chronic thrombocytopenia and concordant neutropenia. | 2001 | 11166667 |
| pure red cell aplasia. | | 2000 | 11167735 |
| the cutaneous pathology of lupus erythematosus: a review. | the presentation of lupus erythematosus (le) ranges from a skin rash unaccompanied by extracutaneous stigmata to a rapidly progressive lethal multiorgan disease. the diagnosis and subclassification is traditionally based on the correlation of serological and clinical findings. the latter include a photoinduced skin rash, arthralgia, arthritis, fever, raynaud's phenomenon, anemia, leukopenia, serositis, nephritis and central nervous sysdtem disease. the conventional classification scheme includes ... | 2001 | 11168747 |
| a syndrome resembling thrombotic thrombocytopenic purpura associated with human parvovirus b19 infection. | this is the first report of a patient with a syndrome resembling thrombotic thrombocytopenic purpura associated with a recent human parvovirus b19 infection. | 2001 | 11170925 |
| [acute generalized exanthematous pustulosis in hypercalcemia]. | we report a case of typical exanthematous pustulosis rash that was particularly severe both clinically and biologically. laboratory tests led to the diagnosis of acute parvovirus b19 infection. | 2000 | 11173687 |
| [macrophage activation syndrome in primary human herpes virus-6 infection: a rare condition after liver transplantation in infants]. | human herpes virus-6 primary infection generally occurs during the first three years of childhood and is generally asymptomatic. the virus has been identified as the causal agent of exanthemum subitum in children or mononucleosis-like disease in adults, and may also cause several disorders in immunocompromised patients. we report a clinical case of acute rejection observed 29 days after orthotopic liver transplantation in a 22-month-old child associated with acute hepatitis and a hemophagocytic ... | 2000 | 11173737 |
| t helper cell-mediated in vitro responses of recently and remotely infected subjects to a candidate recombinant vaccine for human parvovirus b19. | t cell proliferation to human parvovirus b19 antigen was measured in 6 patients with recent b19 infection (1 with pneumonia and pleuritis), 1 patient with symptoms persisting >180 days after onset, 18 nonsymptomatic subjects with remote b19 immunity, and 12 b19-seronegative control subjects. recombinantly expressed virus-like particles (vp1/2 capsids), a candidate b19 vaccine, were used as antigen. virus-specific t helper cell proliferation was detectable in all the recently infected patients an ... | 2001 | 11181158 |
| transient anticardiolipin antibody syndrome in a patient with parvovirus b19 infection. | | 2000 | 11184773 |
| childhood idiopathic thrombocytopenic purpura associated with human parvovirus b19 infection. | | 2000 | 11192536 |
| nosocomial outbreak of parvovirus b19 infection in a renal transplant unit. | parvovirus b19 (b19) infection is known to cause chronic infection leading to anemia in immunocompromised patients. although nosocomial b19 infections in immunocompetent patients have been documented, no outbreaks in immunocompromised patients have been previously reported. whether transmission can occur from a patient with chronic infection is also unknown. | 2001 | 11211196 |
| a quantitative, internally controlled real-time pcr assay for the detection of parvovirus b19 dna. | parvovirus b19 is an erythrovirus causing diverse clinical manifestations ranging from asymptomatic or mild, to more severe outcomes in, for example, immune-compromised patients. b19 is spread primarily via the respiratory route, but it can also be transmitted via blood and blood products. viral loads in blood or plasma donations amount up to 10(11) genome equivalents/ml. therefore, screening of plasma for fractionation for the presence of b19 and removal of highly loaded donations is a way to l ... | 2001 | 11226565 |
| placental cellular immune response in women infected with human parvovirus b19 during pregnancy. | human parvovirus b19 can cause congenital infection with variable morbidity and mortality in the fetus and neonate. although much information exists on the b19-specific antibody response in pregnant women, little information is available describing the cell-mediated immune (cmi) response at the maternal-fetal interface. the focus of this study was to characterize the cmi response within placentas from women who seroconverted to b19 during their pregnancies and compare it to controls. immunohisto ... | 2001 | 11238210 |
| [finding the cause of death in intrauterine death--which examination should be done?]. | the incidence of antepartum fetal death after 22 weeks of gestation is about 0.4% in oslo, norway. screening routines will decide how many cases will remain unexplained. one quarter are diagnosed as sudden intrauterine unexplained death (siud). precise knowledge of the cause of death is needed as a basis for counselling, prevention and treatment. | 2001 | 11242876 |
| parovirus b19 infection in an hiv-infected patient with febrile pancytopenia and acute hepatitis. | the case of a 34-year-old male patient with hiv infection referred for severe febrile pancytopenia is reported. clinical and laboratory evaluations revealed acute hepatitis b infection and concomitant parvovirus b19 infection. the patient died just before treatment with immune globulin was to be administered. parvovirus b19 has been found to cause a wide variety of hematologic disorders such as neutropenia, thrombocytopenia, pancytopenia, and hemophagocytic syndrome. the role of parvovirus b19 i ... | 2001 | 11245322 |
| dermatologic manifestations of infectious diseases in pregnancy. | several pathogens that have been identified as teratogenic or fetotoxic have associated dermatologic changes when active infection is present. viral and bacterial teratogenic pathogens include herpes simplex virus 1, herpes simplex virus 2, varicella-zoster virus, cytomegalovirus, human papilloma virus, human parvovirus b19, rubella, viral hepatitis, syphilis, and gonorrhea. this article focuses on the characteristic dermatologic manifestation of these diseases in pregnancy; diagnostic strategie ... | 2000 | 11249292 |
| human parvovirus b19 infection in patients with systemic lupus erythematosus. | the clinical significance of the presence of b19 dna in patients with sle was studied. | 2001 | 11257151 |
| development of parainfectious opsoclonus in an infant by a non-humoral immune mechanism. | | 2001 | 11263694 |
| resolution of chronic parvovirus b19-induced anemia, by use of highly active antiretroviral therapy, in a patient with acquired immunodeficiency syndrome. | we present what is, to our knowledge, the second case report of a patient with acquired immunodeficiency syndrome and transfusion-dependent anemia caused by persistent infection with parvovirus b19. the patient's anemia was successfully treated, and she demonstrated serologic evidence of eradication of parvovirus after having received effective therapy for human immunodeficiency virus infection. | 2001 | 11264051 |
| parvovirus b19 antibodies in immunocompromized children in thailand. | parvovirus b19, a non-enveloped single stranded dna virus is distributed worldwide. sero-prevalence in adult populations amounts to approximately 50%. clinical manifestations vary depending on the immune status of the infected individuals and may include mild childhood infection as well as hydrops fetalis due to intrauterine infection. to determine the prevalence of this infection among the immunocompromized individuals in thailand, we determined, by indirect elisa, levels of igm and igg antibod ... | 2000 | 11270472 |
| virus inactivation of plasma-derived proteins by pasteurization in the presence of guanidine hydrochloride. | viruses, among them parvovirus b19 and other small, nonenveloped viruses, may be present in human blood and may contaminate plasma-derived therapeutics. efficient inactivation or removal of such viruses, especially parvoviruses, represents a current problem and corresponding technologies are under investigation. in this report, such a technology is described. | 2001 | 11274595 |
| fatal parvovirus myocarditis in a 5-year-old girl. | infection with parvovirus b19 is common in children and typically causes mild illness. we report here the case of a 5-year-old girl who died suddenly, 2 weeks after the clinical diagnosis of a parvoviral infection (erythema infectiosum). microscopic examination of the heart showed severe myocarditis with extensive t-cell and macrophage infiltration. cultures, serology, and molecular analyses of serum for enteroviridae, adenovirus, influenza, varicella zoster, cytomegalovirus, and herpes simplex ... | 2001 | 11274646 |
| neuralgic amyotrophy associated with parvovirus b19 infection in a child. | | 2001 | 11277389 |
| lack of known hepatitis virus in hepatitis-associated aplastic anemia and outcome after bone marrow transplantation. | viral infection has been shown to induce aplastic anemia, unidentified types of hepatitis being the most common cause for aplastic anemia-associated viral hepatitis. the survival rate for this group of patients after bone marrow transplantation with stem cells from an hla-matched sibling is not well known. the aim of this study was to determine the prevalence of hepatitis g virus (hgv) and transfusion transmitted virus (ttv) infection in non-a, non-b, non-c hepatitis associated-aplastic anemia ( ... | 2001 | 11281388 |
| seroprevalence of varicella zoster virus, parvovirus b19 and toxoplasma gondii in a melbourne obstetric population: implications for management. | at an antenatal clinic at a melbourne obstetric hospital, 308 women were questioned about a known past history of infection with varicella zoster virus (vzv), human parvovirus b19 and toxoplasma gondii. immunoglobulin g (igg) concentrations were determined for the 3 infectious agents and a recalled history of infection was compared with the presence of specific antibody. exactly 66% of women recalled being infected with chickenpox (vzv) and 94% showed serological evidence of past exposure. altho ... | 2001 | 11284642 |
| differential igm response to conformational and linear epitopes of parvovirus b19 vp1 and vp2 structural proteins. | the igm immune response against conformational and linear epitopes of b19 structural proteins vp1 and vp2 was examined in serum samples with a suspect b19 infection to determine the most suitable antigen for use in igm detection and also to evaluate a possible relationship between the course of b19 infection and the presence of epitope type-specific igm. the detection of igm against conformational epitopes was performed by elisa using undenatured vp1 and vp2 antigens whereas the detection of igm ... | 2001 | 11285571 |
| recombinant human parvovirus b19 vectors: erythrocyte p antigen is necessary but not sufficient for successful transduction of human hematopoietic cells. | the blood group p antigen, known to be abundantly expressed on erythroid cells, has been reported to be the cellular receptor for parvovirus b19. we have described the development of recombinant parvovirus b19 vectors with which high-efficiency, erythroid lineage-restricted transduction can be achieved (s. ponnazhagan, k. a. weigel, s. p. raikwar, p. mukherjee, m. c. yoder, and a. srivastava, j. virol. 72:5224-5230, 1998). however, since a low-level transduction of nonerythroid cells could also ... | 2001 | 11287560 |
| two family members with a syndrome of headache and rash caused by human parvovirus b19. | human parvovirus b19 infection can cause erythema infectiosum (ei) and several other clinical presentations. central nervous system (cns) involvement is rare, and only a few reports of encephalitis and aseptic meningitis have been published. here, we describe 2 cases of b19 infection in a family presenting different clinical features. a 30 year old female with a 7-day history of headache, malaise, myalgias, joint pains, and rash was seen. physical examination revealed a maculopapular rash on the ... | 2001 | 11290313 |
| neonatal syncytial giant cell hepatitis with paramyxoviral-like inclusions. | syncytial giant cell hepatitis in the neonatal period has been associated with many different etiologic agents and may present initially as cholestasis. infectious causes are most common and include: (1 ) generalized bacterial sepsis, (2) viral agents, (3) toxoplasmosis, (4) syphilis, (5) listeriosis, and (6) tuberculosis. viral hepatitis may be due to cytomegalovirus, rubella virus, herpes simplex, hhv-6, varicella, coxsackievirus, echovirus, reovirus 3, parvovirus b19, hiv, enteroviruses, para ... | 2001 | 11297322 |
| reconstituted immunity against persistent parvovirus b19 infection in a patient with acquired immunodeficiency syndrome after highly active antiretroviral therapy. | we discovered a patient with aids with persistent b19 infection who had slow resolution of anemia after he commenced receiving haart without intravenous immunoglobulin. the patient's anemia recurred when the initial course of haart failed, but it remitted slowly after salvage therapy was instituted. however, circulating b19 was still detectable by nested polymerase chain reaction 1 year after commencement of salvage therapy. immunoglobulin g and immunoglobulin m antibodies against b19 were not d ... | 2001 | 11303273 |
| [pure red cell aplasia due to persistent human parvovirus b19 infection in healthy adult]. | | 2001 | 11307293 |
| a system to enhance the sensitivity of digoxigenin-labelled probe: detection of b19 dna in serum samples. | a highly sensitive dot-blot hybridisation assay for the routine screening of numerous samples is described, using parvovirus b19 as a model. digoxigenin-labelled b19 dna probe was constructed by pcr, hybrids were detected by an anti-digoxigenin monoclonal antibody followed by a second step, using anti-mouse antibodies conjugated to an alkaline phosphatase-dextran complex (envision, dako) was carried out. the sensitivity of the assay was evaluated using both colourimetric and chemiluminescent sub ... | 2001 | 11311352 |
| inactivation of parvovirus b19 in coagulation factor concentrates by uvc radiation: assessment by an in vitro infectivity assay using cfu-e derived from peripheral blood cd34+ cells. | nonenveloped and thermostable viruses such as parvovirus b19 (b19) can be transmitted to patients who are receiving plasma-derived coagulation factor concentrates treated by the s/d method for inactivating enveloped viruses. therefore, it is important to develop and validate new methods for the inactivation of nonenveloped viruses. | 2001 | 11316894 |
| automation of nucleic acid extraction for nat screening of individual blood units. | automation of nat for single units of blood is currently hampered by the labor-intensive steps involved in the extraction of nucleic acids from samples before the amplification procedures. a new method has been developed for the automation of these steps using hydrophilic polyvinylidene fluoride (pvdf) filter plates. | 2001 | 11316898 |
| the historical record is consistent with the recent finding of parvovirus b19 infection of bone marrow in systemic sclerosis patients. | | 2001 | 11326495 |