| macrophages activated as suspension cultures with lymphocyte mediators devoid of antigen become cytotoxic for tumor cells. | normal peritoneal exudate cells (pec) were activated as suspension cultures either in mediator-rich supernatants from o-chlorobenzoyl-bovine gamma-globulin (ocb-bgg) stimulated lymphocytes or in antigen-free sephadex fractions from these supernants. after 24 hr incubation thration. the adherent cell fractions of pec, recovered by trypsinization from monolayers and activated by this technique, were as cytotoxic as unfractionated pec. lymphocyte supernatants and antigen-free fractions of the super ... | 1975 | 50373 |
| bovine keratohyalin: anatomical, histochemical, ultrastructural, immunologic, and biochemical studies. | salt extraction studies showed that keratohyalin (kh) could be solubilized and extracted from fresh bovine hoof epidermis. the solubility of kh varied in relation to the molarity of the salt solution used for extraction. using this information, the extracted kh was aggregated in vitro by dialyzing the high salt extract against distilled water. histochemical, ultrastructural, and immunologic studies of the resultant particles or macroaggregates showed that the latter had the same properties and i ... | 1975 | 50383 |
| a radioimmunoassay for myelin basic protein and its use for quantitative measurements. | | 1975 | 50409 |
| possible association of oncornavirus type d with some forms of human cancer. | the method of molecular hybridization of nucleic acids of oncornavirus type d produced by hep2 cells with cellular nucleic acids was applied. no sequences homologous to the viral nucleic acids were found in normal human embryo cells as well as in bovine, porcine, murine, and chicken cells. no such sequences were found in tissues of malignant lymphomas. sequences homologous to the viral nucleic acids were found in some cancer cells, predominantly in hormone-associated tumors of females. | 1975 | 50567 |
| [serologic tests with the use of encephalitogenic protein in the diagnosis of multiple scerosis]. | | 1975 | 50571 |
| biologic and immunologic properties of porcine type c viruses. | | 1975 | 50668 |
| production of anaphylactic antibodies to drugs in experimental animals. i. benzylpenicillin. | studies were undertaken to determine if animals exposed to benzylpenicillin could produce anaphylactic antibodies to the drug moiety. for this purpose, albino wistar rats were divided into four groups. two groups of animals were immunized with benzylpenicillin using either the intraperitoneal or the subcutaneous route. the remaining two groups were used for feeding experiments. the animals were bled upon termination of the experiments; their sera were collected and pooled per group. the pooled s ... | 1975 | 50703 |
| comparison of rna-directed dna polymerases from xenotropic and ecotropic viruses. | | 1975 | 50842 |
| study of the antigenic cross reactivity between carcinoembryonic antigen and "nonspecific cross reacting antigens" (nca and nca 2). | the immunochemical relationship between cea, nca and nca 2 was studied in guinea-pigs. strong cross reactions were found between these antigens, either in delayed or anaphylactic reactions. some specific determinants for each antigen could still be demonstrated. delayed hypersensitivity is likely to be due to the protein moiety of the molecules while anaphylactic reactivity could probably be related to their glucidic parts. consequently, cea and nca have common antigenic determinants on their gl ... | 1975 | 50854 |
| the interaction of concanavalin a and the surface coat of stratified squamous epithelium. | the interaction of concanavalin a (con a) and the surface membranes of the epithelial cells of human oral mucosa, epidermis and guinea-pig lip was studied. two methods were used: fluorescent con a and con a in combination with horse radish peroxidase. with both methods the surface coat of epithelial cells was stained. the specificity of the staining methods could be demonstrated. the interaction between pemphigus antibodies and the surface membranes of epithelial cells could be blocked by con a. | 1975 | 50858 |
| [polyacrylamide gel block electrophoretic study of the blood serum of adult swine]. | | 1975 | 50863 |
| immunobiology of tuberculin hypersensitivity. | | 1975 | 50915 |
| [use of the immune tolerance induction in the production of an antilymphocyte serum (als) free of antibodies against serum proteins]. | the possibility has been investigated of a direct gain of als free of undesirable antibodies against serum proteins by inducing immunologic tolerance in productive animals (pigs). preliminary experiments made with tolerogenic amounts of 10 and 50 ml of sera and with immunization by the serum alone proved applicability of this method. electrophoresis showed antibodies against 6 to 7 and 2 to 3 fractions in animals tolerated with 10 and 50 ml respectively, compared to 18 to 20 fractions in the con ... | 1975 | 50969 |
| stimulation of adenylate cyclase activity in retro-orbital tissue membranes by thyrotropin and an exophthalmogenic factor derived from thyrotropin. | retro-orbital tissue membranes have been shown to have adenylate cyclase activity which can be stimulated by thyrotropin and by an exophthalmogenic factor derived from the thyrotropin molecule by partial pepsin digestion. this stimulable activity is maximal after 15 min and is optimal in the presence of 3 mm magnesium and 1.5 mm atp. calcium salts are exquisitely inhibitory to the hormonal stimulation; sodium, lithium, and ammonium salts are significantly less inhibitory. thyrotropin and the exo ... | 1975 | 51022 |
| [method of quantitative determination of trypsin and the activity of antitrypsin with the aid of specific antitryptic precipitating serum]. | | 1975 | 51116 |
| the reproducibility of reactions obtained with cytotoxic antisera directed against various dl-a specificities. | | 1975 | 51522 |
| preparation of monospecific antiserums against porcine immunoglobulins, using agarose-linked immunosorbents. | immunoglobulins igg, iga, and igm were isolated from porcine serum and milk, and antiserums against the 3 immunoglobulin classes were prepared. monospecificity of the antiserums for the gamma-, alpha-, and mu-chains was obtained by absorbing them in agarose-linked immunosorbent columns. these immunosorbents were prepared by linking igg or iga-igm to cnbr-activated agarose. contaminating anti-alpha2-macroglobulin antibodies in the anti-iga and anti-igm serums were removed with agarose-linked feta ... | 1975 | 51601 |
| morphological, chemical, and antigenic organization of mammalian c-type viruses. | new features in the architecture of mammalian type c viruses, in particular knoblike surface projections and hexagonally arranged subunits on the core shell could be demonstrated by electron microscopy, taking advantage of newly developed preparation techniques. as examples, murine leukemia viruses (mulvs) and newly isolated porcine and bovine c viruses are presented. the major proteins of a mulv were isolated and partially characterized in chemical terms and with respect to their serological an ... | 1975 | 51634 |
| effects of barbiturates on ultrastructure and polymerization of microtubules in vitro. | barbiturates were examined for in vitro effects on ultrastructure of the frog sciatic system and polymerization of microtubules (mt) in a brain supernatant. exposure for 5-17 h to 2.0 mm barbiturates caused a considerable loss of mt in ganglionic cell bodies and sciatic axons. this was mostly followed by a proliferation of 10 nm filaments. under similar conditions treatment with 1 mm nacn or 0.1 mm 2,4-dnp did not change the number or ultrastructure of mt and filaments. eight barbiturates, varyi ... | 1975 | 51688 |
| inhibition of t- and b-lymphocyte functions by normal immunosuppressive protein. | normal immunosuppressive protein (nip) isolated from human plasma was studied in two well defined systems. (1) spontaneous rosettes of sheep red blood cells with human peripheral blood lymphocytes and pha-induced lymphocyte cytotoxicity as indicators for t-cell function. (2) rosette formation tests of human lymphocytes with antibody-coated erythrocytes or erythrocytes coated with antibody and complement as well as antibody-induced lymphocyte-mediated cytotoxicity represented non-t-cell activity. ... | 1975 | 51828 |
| relationships between mitochondrial content and glycogen distribution in porcine muscle fibres. | serial sections of longissimus dorsi and rectus femoris muscles from 15 yorkshire breed pigs (live weights 24-46 and 49-139 kg) were stained for glycogen (pas) and a mitochondrial enzyme (nad tetrazolium reductase). muscle fibres with a low mitochondrial content in both muscles were more frequently pas-positive than fibres with a high or intermediate mitochondrial content. however, some pigs had all their muscle fibres pas-positive while one pig with a high post-mortem muscle ph had all rectus f ... | 1975 | 51844 |
| the isolation of allergens from the green pea. | the aqueous extract of green peas was separated into 3 fractions (albumin, legumin, and vicilin) by dialysis against distilled water and isoelectric precipitation. the major antigenic and all of the allergenic activity of the pea extract was associated with the albumin fraction. the albumin fraction retains its allergenicity upon heating at 60 degrees c for 30 min or boiling at 100 degrees c for 5 min, but becomes partially inactivated by autoclaving at 120 degrees c for 15 min. the allergenic d ... | 1975 | 51864 |
| the antibody responses to myelin basic protein (bp) in lewis rats: the effects of bordetella pertussis. | a time-course study was made of the systemic humoral immune response of lewis rats to myelin basic protein (bp) as influenced by the dosage of ancillary pertussis adjuvant. peak activities were observed 5 to 7 weeks after injection. when injected proximal to bp and mycobacterium butyricum in complete freund's adjuvant (cfa), bordetella pertussis at the level of 4 billion organisms doubled the antibody-binding activity of rat sera for 125i-labeled bp as compared to activities obtained with 0, 2, ... | 1975 | 51876 |
| inhibition of macrophage-lymphocyte interaction by cytochalasin b during antigen recognition by t lymphocytes. | the effects of cytochalasin b on functional and physical macrophage-lymphocyte interaction have been examined. cytochalasin b, an inhibitor of a variety of membrane activities blocks antigen-dependent bindings of immune lymphocytes to macrophages and antigen-triggered lymphocytes proliferation if added at the initiation of culture. cytochalasin b becomes progressively less inhibitory if addition is delayed by increasing intervals from the onset of culture. under these conditions neither antigen ... | 1975 | 51878 |
| [biochemical properties of alpha-fetoprotein (author's transl)]. | | 1975 | 51943 |
| editorial: noise-induced hearing loss. | | 1975 | 51968 |
| non-encephalitogenic antigen-induced suppression and reversal of allergic encephalomyelitis. | | 1975 | 52118 |
| disease-inducing activity of different parts of bovine encephalitogenic protein in guinea-pigs. | the encephalitogenic activity of different parts of bovine encephalitogenic protein (bep) has been tested in guinea-pigs, mainly at a dose equimolar to 32 mug of intact protein. a high activity was found with complete protein and with region 89-169; both peptide 43-115, tyr (modified at the tyrosine residue 67) and hnb-89-169 (modified at the tryptophan residue 115) showed a moderate but clear-cut activity, whereas that of peptide 1-42 was very faint. no qualitative differences in the disease in ... | 1975 | 52129 |
| [induced systemic lupus erythematosus]. | | 1975 | 52171 |
| immunologic differentiation of proteins in the surfactant system of the lung. | immunoelectrophoresis revealed the following four protein components in the lung surfactant system of guinea-pigs: albumin, transferrin, igg, and an organ-specific protein migrating in the beta-globulin range. these proteins probably take part in the formation of the hypophase of the alveolar lining layer and thereby influence the surface properties of the alveolar air-liquid interface. | 1975 | 52192 |
| leishmaniasis in brazil: x. some observations of intradermal reactions to different trypanosomatid antigens of patients suffering from cutaneous and mucocutaneous leishmaniasis. | in spite of the difficulty of antigen standardization, paired intradermal skin tests demonstrated qualitative differences between leishmanin and in vitro exo-antigen. some of these differences may be due to the destruction of certain antigens by phenol, while others seem to reflect basic antigenic differences. leishmania mexicana amazonensis in vitro exo-antigen produced immediate anaphylactic reponses in 74.4% of the patients with parasitologically proven cutaneous leishmaniasis. although more ... | 1975 | 52214 |
| gordon wilson lecture, 1974: searching for the cause of systemic lupus erythematosus. | | 1975 | 52230 |
| [neuraminidase neutralizing antibodies in pigs with chronic erysipelothrix rhusiopathiae infection (author's transl)]. | investigating sera from pigs with chronic e. rhusiopathiae infection relative gamma-globulin values, agglutination titers, and neuraminidase-neutralizing humoral antibodies were determined at the 12th and 15th, 11th and 14th, and 3rd and 6th month after infection. we found increasing gammaglobulin values and increasing neuraminidase neutralizing antibodies but decreasing agglutination titers. few animals don't have antibodies against neuraminidase and died during the observation period. furtherm ... | 1975 | 52247 |
| products of epidermal protein synthesis. | the products of epidermal protein synthesis in tissue slice and cell-free systems have been characterized by chromatographic, electrophoretic, and immunologic methods. radiolabeled proteins of heterogeneous size are found in the supernatant and particulate fractions from both systems. the major labeled proteins in the supernatant fraction have molecular weights of approximately 43 000 and 65 000. the natively-insoluble proteins of the particulate fraction were solubilized either with desoxychola ... | 1975 | 52256 |
| anti-basement membrane antibodies in sera from patients without bullous pemphigoid. | sera from 208 individuals were examined for antibasement membrane (anti-bm) antibodies by means of an indirect immunofluorescence technique. in sera from patients with bullous pemphigoid 19 of 25 had anti-bm antibodies in titres varying from 80 to 32 000. in 2 of 42 sera from patients with psoriasis, anti-bm antibodies were demonstrated (titres 40-640). of 58 sera from patients with leg ulcers, antibodies were found in 4 (titres 40-320). antibodies were found in 2 of 43 patients with cardio-vasc ... | 1975 | 52261 |
| thyroxine utilization rate in sucking pigs. | in 10 suckling pigs age 28--34 days, thyroxine utilization rate (tur) was determined by means of the isotope dilution procedure. for this purpose biological half-life of t4 was estimated during 96 h of blood sampling and serum t4 concentration was measured directly by a competitive protein-binding technique. high rate of the peripheral hormonal-iodine metabolism (from 10.3 +/- 0.6 to 21.2 +/- 1.2 mug t4/kg/day) was found in the one-month old pigs. tur value calculated for these pigs corresponds ... | 1975 | 52283 |
| analysis of cell populations with a fluorescence-activated cell sorter. | | 1975 | 52308 |
| detection by immunofluorescence of antibodies to parasitic agents. use of class-specific conjugates. | | 1975 | 52314 |
| microfluorometric evaluation of conjugate-specificity with the defined antigen substrate spheres (dass) system. | six fluorescent antihuman ig preparations were tested for their ig class specificity by reacting them with highly purified igg, igm, iga, and ova coupled covalently to sepharose beads. ova was used as a measure for nonimmunologic binding. bead fluorescence was determined by microfluorometry. the amounts of uss and nss were expressed quantitatively. these data were compared with the performance of these particular conjugates in a biologic system, namely, monoclonal bone marrow cells. five of the ... | 1975 | 52328 |
| properties of bispecific rosette-forming cells. i.--presence of t-lymphocyte markers. | three t-cell markers have been used to determine the origin of bispecific rosette forming cells (rfc) observed after stimulation by two species of heterologous red cells. the inhibition by anti-theta serum (aos), azathioprine (az) and anti-lymphocyte serum (als) of the majority of bispecific rfc indicates their t-cell origin. the study of their sensitivity to these inhibitors suggested that about half of the bispecific rfc belong to immature t1 cell subset and the other half to more mature t2 ce ... | 1975 | 52332 |
| correlation of cytotoxicity with experimental allergic encephalomyelitis. | | 1975 | 52410 |
| the influence of c3b inactivator (kaf) concentration on the ability of serum to support complement activation. | minor elevation of the concentration of the c3b inactivator (kaf) in whole serum (by 15-25%) markedly inhibits the capacity of the serum to support complement activation by inulin, aggregated igg and by low concentrations of cvf. however, there was no effect when large concentrations of cvf were used nor was the spontaneous ageing of c3 slowed by increased kaf concentrations. these findings show that the activity of the c3b feedback cycle can be reduced by raising the kaf concentration above phy ... | 1975 | 52423 |
| accelerated cytodifferentiation of antibody-secreting cells in guinea-pig lymph nodes stimulated by sheep erythrocytes and lymphokines. | lymphokines, produced in response to structurally unrelated antigens, altered the course of a primary anti-sheep erythrocyte plaque-forming cell response within the regional lymph nodes of normal guinea-pigs. intralymphatic injection of a small dose of lymphokines (0-5-8 mug) 1 day after antigen priming accelerated the rate of indirect plaque-forming cell cytodifferentiation between the 5th and the 9th days of the response. this effect was not related to changes in the level of antigen trapping ... | 1975 | 52424 |
| experimental autoallergic myositis, polymyositis and myasthenia gravis. autoimmune muscle disease associated with immunodeficiency and neoplasia. | | 1975 | 52425 |
| depression of complex bioelectric discharges in cerebral tissue cultures by thermolabile complement-dependent serum factors. | | 1975 | 52559 |
| control of ribonucleic acid polymerase a activity during lymphocyte activation by phytohaemagglutinin. | | 1975 | 52565 |
| physical studies of tissue anaphylaxis. | | 1975 | 52596 |
| the chemical characterization of a major allergen of timothy pollen. | | 1975 | 52600 |
| the nature of processed antigens in immune responses. | | 1975 | 52608 |
| on the pharmacology of org 6338 (2beta, 16beta-dipiperidino - 5alpha - androstan - 3alpha - ol acetate dimethobromide), a new steroidal neuromuscular blocking agent. | org 6368 is a homologue of pancuronium bromide. its interactions with other agents in the cat sciatic nerve-gastrocnemius muscle preparation revealed that paralysis was of the non-depolarizing type. this was confirmed in experiments using avian muscle. org 6368 is a potent muscle relaxant being 2-4 times as potent as (+)-tubocurarine in the cat. paralysis in the cat is rapid in onset and of appreciably shorter duration than that of pancuronium and (+)-tubocurarine. repeated injections of the sam ... | 1975 | 52704 |
| effect of ascorbic acid on histamine metabolism in scorbutic guinea-pigs. | 1. in guinea-pigs fed an ascorbic-acid-free diet, as the ascorbic acid levels decreased the histamine levels in blood and urine rose steadily to maxima in about 10-12 days. the elevated histamine levels persisted in the blood and urine of scorbutic guinea-pigs and the histamine levels in lung, gastric mucosa and spleen also increased. the increased histamine content of the urine, blood and other tissues in the ascorbic-acid-depleted condition could be brought back to normal levels by administrat ... | 1975 | 52707 |
| glycosaminoglycans from ateroid and bovine duodenal mucosa and pancreas. | glycosaminoglycans (gg) were isolated from commercial ateroid and compared with those from bovine duodenal mucosa and pancreas. the major gg in ateroid is heparin. heparan sulfate (hs) and dermatan sulfate were also found. hs, chondroitin sulfates, and heparin were isolated from duodenal mucosa after papain digestion, but a residue, non-digestible, was mostly heparin. pancreas contains very little gg, and the gg composition is similar to that of mucosa. the heparin isolated from ateroid and muco ... | 1975 | 52831 |
| decreased myelin basic protein content of the aged human brain. | myelin basic protein was extracted from frontal lobe white matter obtained at autopsy from seven individuals ranging in age from 72 to 78 years. qualitative and quantitative comparisons were made between basic protein from this group and that extracted from another group of individuals ranging in age from 45 to 65 years. the basic protein content of the older brains was considerably lower than that of the younger brains. qualitative studies that included assay for encephalitogenic activity, disk ... | 1975 | 52852 |
| why are white lesions white? observations on keratin. | it is proposed that oral keratoses appear white because of the ability of abnormal oral keratin to evenly reflect the visible light spectrum because of the hydration of the keratin layer in a manner similar to the reaction of the stratum corneum of the epidermis to water. thickening of the keratin layer per se or the over-all thickness of the epithelium does not appear to be the primary factor in causing an intraoral lesion to appear white. the white appearance is related to thickness only insof ... | 1975 | 52857 |
| [immunohistochemical studies on non neoplastic and neoplastic gastric mucosa. determination of embryonic and specific antigens (author's transl)]. | the distributions of acid alpha1-glycoprotein, alpha1-fetoprotein, beta-galactosidase and gastrin in gastric carcinoma and gastric ulcer as well as in the neighbourhood of these lesions were studied by means of immunohistochemical methods on imprint preparation. we could not find significant differences between gastric carcinoma and the nonneoplastic lesions, except for the acid alpha1-glycoprotein. the results of this first study indicate that the immunochemical and immunohistological assay of ... | 1975 | 52951 |
| [experiences with the macrophage electrophoresis mobility test (mem test) in the diagnosis of malignant gynecological diseases]. | in 1970, field and caspary reported that lymphocytes from patients with a malignant disease could be stimulated by an encephalitogenic factor, the myelin basic protein from human brain. after in vitro incubation with this protein the stimulated lymphocytes release a factor ("macrophage slowing factor") which reduces the electrophoretic mobility of guinea-pig macrophages. in general, this effect was not found in lymphocytes from patients with non-malignant disease. on the base of this unexpected ... | 1975 | 52961 |
| [the histochemical evidence of the cholinesterase in the integument by means of the copper ferro cyanide methode (author's transl)]. | with the aid of the ferrocyanidmethod from karnovski und roots che-positiv structures in the skin of various laboratory animals and of man were located. specific cholinesterase is found in verves, around apocrine sweat glands and around eccrine sweat glands of some species. in the stratum basale of the epidermis from the rat you can demonstrate a high concentration of acetylcholinesterase. unspecific cholinesterase is detectable in nerves, in the blood vessels, in the lumen of apocrine sweat gla ... | 1975 | 52982 |
| comparison between narcotic "receptors" in the guinea-pig ileum and the rat brain. | | 1975 | 52996 |
| biophysical and biochemical characterization of hepatitis b antigen. | hepatitis b surface antigens specified by the genome of the hepatitis b virus are shared by various particulate forms which circulate in the sera of chronic hbs ag carriers. as purified from sera, hbs ag consists of at least seven polypeptides, two of which appear to be glycoproteins. most or all of these polypeptides contain both group-specific (a) and subtype-specific (d or y) determinants aspart of their structure. one particulate form, the dane particle, is present as a minor component in mo ... | 1975 | 53004 |
| hepatitis b antigen subtypes-history, significance and immunogenicity. | initial work showed that all hepatitis b surface antigens (hbs ag) were identical. subsequent, additional studies revealed that hepatitis b surface antigens have a group reactive determinant, a, plus additional specificities which are not universally present on all antigens. four well-defindd subtypes of hbs ag exist (hbs ag/adw, hbs ag/ayw, hbs ag/adr, hbs ag/ayr) but additional subtypes will be forthcoming as newly described determinants are confirmed. the subtype specificities are determined ... | 1975 | 53007 |
| relationship of hepatitis a antigen to viral hepatitis. | progress in research on hepatitis type a has begun to accelerate because of the recent discovery of an antigen associated specifically with hepatitis type a infection and the development of tests for antibody to the antigen. hepatitis a antigen is associated with 27 nm virus-like particles found in the liver and stool of animals experimentally infected with hepatitis type a and in the stool of humans experimentally or naturally infected with the virus. the density of the particulate antigen when ... | 1975 | 53012 |
| development and utilization of complement-fixation and immune adherence tests for human hepatitis a virus and antibody. | the reliable propagation of cr326 strain of human hepatitis a virus in saguinus mystax marmosets has permitted the development of specific serum neutralization, complement-fixation (cf), and immune adherence (ia) assays for hepatitis a antigen and antibody. the cf and ia assay were made possible by the use of livers of cr326-infected marmosets as a source of hepatitis a antigen. all assays were shown to be specific for hepatitis a. patients with hepatitis b did not show development of hepatitis ... | 1975 | 53013 |
| the ultrastructure of a characteristic spermhead-defect in the boar: the sme-defect. | semen samples from an eight-month-old landrace boar showing poor sperm motility were investigated at the boars' arrival to an a.i. centre. sperm morphology was found abnormal in +/- 30% of the sperm heads, which contained a rounded, often circular hypochromatic body (eosin-nigrosin stain) about 2 mum in diameter. the body was located in the front half of the sperm head, i.e. within the region of the acrosomal cap. later a similar defect (+/- 50%) was found in an imported 2 1/2-year-old yorkshire ... | 1975 | 53019 |
| the differential binding of antibody from the sera of patients with pemphigus and pemphigoid to isolated guinea-pig epidermal cells. | a method for preparing isolated epidermal cells using trypsin is described. the isolated cells retain the ability to bind antibody from patients with pemphigus or with pemphigoid in a disease-specific. | 1975 | 53062 |
| immunochemical study of beta-glucuronidase inhibitor from porcine sublingual gland. interaction of beta-glucuronidase inhibitor with alpha2-macroglobulin. | antiserum to the inhibitor of beta=glucuronidase isolated from porcine sublingual gland was prepared in rabbits. double immunodiffusion with the inhibitor produced a single precipitin line. however, neutralization of the inhibitor was produced by the antiserum and also by normal serum. anti-beta-glucuronidase inhibitor isolated from human serum, by fractionation with (nh4)2 so4 followed by deae-cellulose, sephadex g-200 and sepharose 4b chromatography, was identified as alpha2-macroglobulin by u ... | 1975 | 53072 |
| gomori-positive elementary granules in inner and outer layer of the infundibulum. | with the gallocyanine technique (wittkowski, bock and franken, 1970) gomori-positive substances of the infundibulum can be stained for light- and electron-microscopic examination. in various mammalian species, the size of gomori-positive elementary granules in the outer layer is markedly different from that in the inner layer of the infundibulum. in general, the granules of the outer layer have less then half the diameter of those of the inner layer. in birds and fish, however, only small differ ... | 1975 | 53100 |
| monographs on fragrance raw materials. | | 1975 | 53171 |
| preparation and evaluation of antisera directed against cancer specific moiety of antigenic determinants on carcinoembryonic antigen. | | 1975 | 53193 |
| secretory component of the guinea-pig. | free secretory component (fsc) was purified from guinea-pig milk by gel-filtration and immunoabsorption on anti-sc antibodies. guinea-pig fsc cross-reacted with antisera to human fsc, and the reverse. guinea-pig and human fsc resembled each other in molecular size, electrophoretic mobility and heterogeneity, as well as by the existence of antigenic determinants restricted to the unassociated form of the molecule. guinea-pig fsc associates in vitro with guinea-pig igm. distribution of disulphide ... | 1975 | 53196 |
| hapten and carrier specificities of cellular and humoral responses to highly substituted dinitrophenyl-human gamma-globulins in guinea-pigs. | delayed hypersensitivity (dh) and antibody reactions to the carrier and to the hapten have been studied in guinea-pigs immunized with different doses of highly substituted dinitrophenyl-human gamma-globulins (dnp56-hgg) in freund's complete adjuvant (fca). the results confirm that dh reactions are specific for the carrier while antibody-mediated reactions are specific for the hapten in the early stages of the immune response. later in the response, however, dh reactions to the hapten as well as ... | 1975 | 53199 |
| purification and serological characterization of a type-specific antigen of streptococcus equisimilis. | a microtiter complement fixation (cf) procedure was developed for use in detection of antibodies in sera of swine and rabbits vaccinated with streptococcus equisimilis. crude ultrasonic as well as acid-extracted preparations contained cf antigen, but the ultrasonic procedure resulted in a higher yield of active antigen. evidence of serotype specificity with varying degrees of cross reactivity was detected with the cf procedure when representative strains of four different serotypes of s. equisim ... | 1975 | 53203 |
| a comparison of the encephalitogenic activities of human myelin basic protein and the synthetic peptide determinant comprising residues 114-122. | | 1975 | 53275 |
| letter: particles associated with microvillous border of intestinal mucosa. | | 1975 | 53415 |
| mucosal adherence of human enteropathogenic escherichia coli. | an in-vitro assay system has been developed to measure bacterial adhesion to the mucosa of human fetal small intestine. two strains of escherichia coli that are proven human enteropathogens (e.p.e.c.) have been shown to adhere in large numbers, compared with control organisms. the attachment mechanism is species specific and is not caused by common fimbriae. mucosal adhesion may be as important as enterotoxin production or invasiveness in determining the virulence of some strains of human e.p.e. ... | 1975 | 53431 |
| identification of cholecystokinin-secreting cells. | cholecystokinin (c.c.k.)-producing cells were identified in the mucosa of the upper small intestine in man and other mammals by immunocytochemical techniques and electron microscopy on semi-thin and serial thin sections. ultrastructural studies suggested that the c.c.k.-producing cells resemble i cells of the modified wiesbaden classification. the combination of these techniques provides a simple and reliable method of identifying c.c.k. cells in human biopsy material and will be useful in the i ... | 1975 | 53500 |
| letter: septicaemia due to porcine streptococci. | | 1975 | 53712 |
| [synthesis of atp by reversal of cation transport atpase (author's transl)]. | | 1975 | 53861 |
| the practice laboratory: the interpretation of blood smears. | | 1975 | 53937 |
| [effect of passive immunity on subsequent vaccination against swine fever]. | studied was the effect of a serum paratyph and aujeszky gammaglobulin injected 10, 15, and 20 days prior to the crystal-violet and the lapinized vaccine. if the animals were infected with a pathogenic swine fever virus 10 to 15 days after their treatment with serum at the rate of 0.5 cu. cm per kg, part of them died, and the remaining contracted the disease showing a protracted course. the control vaccination on the 20th day after the animals were injected with serum led to the death of all of t ... | 1975 | 53938 |
| [proceedings: antibodies against myocardial sarcolemma in primary cardiomyopathies]. | | 1975 | 53951 |
| immunological and chemical correlation between alpha-fetoproteins from human and several mammalian species. | alpha-fetoproteins of several animals were purified and their molecular weights, amino acid compositions and peptide maps were compared, demonstrating the close similarities. these data indicated that the alpha-fetoproteins of mammalian species have closely related antigenical and chemical structures. rabbits and horses were immunized with human alpha-fetoprotein, and it was observed that the animals produced antibodies reaction not only with human alpha-fetoprotein but with their homologous alp ... | 1975 | 54014 |
| individual semicircular canals and pontine reticular units. | | 1975 | 54052 |
| proceedings: evidence for rigor in smooth muscle. | | 1975 | 54073 |
| percutaneous absorption of 14c-9alpha-fluoro-11beta,21-dihydroxy-3,20-dioxopregna-1,4-dieno (17alpha,16alpha-d)-2'-methyloxazolidine-21-acetate (l 6400) in rats, pigs and humans. comparison with 3h-triamcinolone acetonide. | 1. the percutaneous absorption of a novel oxazolino corticosteroid, 14c-9alpha-fluoro-11beta,21-dihydroxy-3,20-dioxopregna-1,4-dieno[17alpha,16alpha-d]-2'-methyloxazolidine-21-acetate (l 6400) and 3h-triamcinolone acetonide (ta) was compared in rats, pigs and humans after application of a cream formulation to the skin and occlusion. 2. within 96 h, up to 4.0% of the applied dose of l 6400 (permeability constant kp 0.28) and up to 8.7% of ta (kp 0.42) were percutaneously absorbed in rats. 3. micr ... | 1975 | 54172 |
| studies on the inhibition of macrophage migration induced by soluble antigen-antibody complexes. | mixtures of serum of freund's complete adjuvant (fca) immunized guinea-pigs and tuberculin ppd consistently inhibited the in vitro migration of peritoneal exudate cells (pec) of normal guinea-pigs. it is shown that this inhibitory effect is due to a soluble complex between an igg2 antibody and ppd. by separation of ppd on sephadex g-200 two peaks were obtained, corresponding respectively to molecular weights of at least 800,000 and 25,000, separated by a plateau. material derived from both peaks ... | 1975 | 54230 |
| species specificity of thromboplastin. a phylogenetic study. | having studied the influence of thromboplastin preparations derived from cold and warm-blooded species on the plasma of 12 vertebrates (carp, frog, turtle, hen, rabbit, rat, mouse, guinea-pig, ground squirrel, dog, sheep and man) we have established that among the species far from each other phylogenetically, the phenomenon of species specificity could be demonstrated in general. it was found that the extrinsic coagulation system measured by quick times can well be activated in every examined sp ... | 1975 | 54309 |
| [cytochemical study of the substrate responsible for the differential staining of human chromosomes by length]. | | 1975 | 54381 |
| antigenic determinants of adenovirus capsids. i. measurement of antibody cross-reactivity. | evidence is presented that the type-specific antibody to the adenovirus hexon is not simply the antibody with the highest activity for cross-reactive determinants, but is a distinct, minority population that recognizes seperate determinants. to quantify it, we have developed an inhibition method with radio-immunoprecipitation (rip) as a sensitive assay for the type-specific antibody that remains after all the excess of cross-reactive antibody has been blocked by heterologous antigen. during the ... | 1976 | 54385 |
| antigenic determinants of adenovirus capsids. ii. homogeneity of hexons, and accessibility of their determinants, in the virion. | we have tested the two principal theories which explain the previous finding that small amounts of type-specific antibody to the adenovirus hexon can neutralize infectivity, whereas even large amounts of cross-reactive antibody do not. a) it has been suggested that the type-specific determinants are especially prominent in the virion. we have therefore measured the capacity of whole virus to bind appropriate antibodies, using a sensitive radioimmunoprecipitation (rip) system. in fact, virions bo ... | 1976 | 54389 |
| approaching the control of viral hepatitis type b. | | 1976 | 54398 |
| effects of in-vitro treatment of boar spermatozoa with tepa on the fertilization and development of pig eggs. | | 1975 | 54425 |
| [modification of the method of differential intracellular staining of living and dead mycobacteria]. | | 1975 | 54479 |
| synthesis of adenosine triphosphate by way of potassium-sensitive phosphoenzyme of sodium, potassium adenosine triphosphatase. | the sodium and potassium ion pump is an intrinsic enzyme of plasma membranes. in these experiments it was driven backward in a transient two-step operation involving, first, phosphorylation of the enzyme from inorganic phosphate, and second, transfer of the phosphate group from the enzyme to adp upon addition of a high concentration of na+. there was no evidence of a significant concentration gradient across the membranes. na+ presumably reached the solutions on both faces of the membrane simult ... | 1975 | 54512 |
| letter: insulin resistance during continuous insulin infusion. | | 1976 | 54551 |
| pathogenesis of enteritis necroticans in papula new guinea. | it is suggested that enteritis necroticans occurs in the people of the highlands of papua new guinea because they have low levels of digestive proteases in the intestinal lumen. these low protease levels are the result of a low-protein diet and the presence of heat-stable trypsin inhibitors in sweet potatato, the dietary staple. low proteolytic activity allows the b toxin of clostridium welchii type c, which is extremely susceptible to proteolysis, to initiate the disease. | 1976 | 54640 |
| evidence of a viral aetiology in endemic (balkan) nephropathy. | segemental and focal pathological changes were found in the glomeruli and tubules of postoperative renal-biopsy specimens from seven cases of clinically confirmed endemic (balkan) nephropathy. in the glomeruli, there was mesangial reaction and segmental thickening of the basement membrane with subendothelial and membranous depositions. in the tubules there was spongiform degeneration and fusion of cells. in all the cells of the nephron numerous cytoplasmic vesicles containing free and budding pa ... | 1975 | 54796 |
| [study of the antigenic relationship of different strains of the rabies group viruses]. | in neutralization tests in animals close antigenic relationship between human virus of acute encephalomyelitis (aem) and viruses (297 and 598 sg strains) isolated from wild rodents in czechoslovakia was first established. at the same time, poor antigenic relationship of human aem virus to viruses isolated in africa from bats (lagos bat) and from shrews (yban 27377) was demonstrated. certain differences in the antigenic properties of viruses isolated in africa and in czechoslovakia were found. t ... | 1975 | 54997 |
| the histocompatibility-linked immune response genes. | | 1975 | 55062 |
| selection of biochemically variant, in some cases mutant, mammalian cells in culture. | | 1975 | 55063 |
| neutrophil granules: a review. | hematologists are familiar with the granules of leukocytes because they serve as a means of identification of certain white blood cells.19 it is now generally appreciated that the granules of neutrophilic polymorphonuclear leukocytes (pmn) store antibacterial agents and lysosomal enzymes until they are needed for the killing and digestion of phagocytized microorganisms.16 within the framework of this well-known setting, this article presents some new data on the mode of formation and chemical co ... | 1976 | 55073 |