| [study of a new anaerobic species: eubacterium sarcosinogenum n.sp]. | | 1960 | 13836465 |
| [the c-reactive protein demonstrated by the polystyrene latex method]. | | 1960 | 13836783 |
| [influence of roentgenotherapy with grids on c-reactive protein]. | | 1959 | 13836988 |
| [the test of the reaction to c-reactive protein in benign and malignant tumors of the female genital apparatus]. | | 1959 | 13836989 |
| kenkel h: relationship of sialic acid and c-reactive protein levels in human serum. | | 1959 | 13840007 |
| c-reactive protein in the serum in nephrosis and acute glomerulonephritis. | | 1960 | 13840942 |
| [behavior of the antistreptolysin o and the "c-reactive protein" titers in some diseases of childhood]. | | 1959 | 13841534 |
| [observations on the behavior of c-reactive protein in the puerperium]. | | 1960 | 13842200 |
| [diagnostic value of c-reactive protein in acute coronary insufficiency]. | | 1960 | 13845775 |
| the c-reactive protein in childhood tuberculosis. | | 1960 | 13847655 |
| [creactive protein in ischemic cardiopathy]. | | 1960 | 13854281 |
| [preliminary trials on the c-reactive protein in ophthalmology]. | | 1959 | 13854588 |
| some observations on the nitrogen metabolism of rumen proteolytic bacteria. | | 1963 | 14010700 |
| some nutritional characteristics of predominant culturable ruminal bacteria. | bryant, m. p. (u.s. department of agriculture, beltsville, md.) and i. m. robinson. some nutritional characteristics of predominant culturable ruminal bacteria. j. bacteriol. 84:605-614. 1962.-the effect of enzymatic hydrolysate of casein, nh(4) (+), a mixture of volatile fatty acids (acetic, n-valeric, isovaleric, 2-methylbutyric, and isobutyric), hemin, and ruminal fluid on growth of 89 freshly isolated strains of predominant culturable ruminal bacteria was studied, using basal media containin ... | 1962 | 14016429 |
| role of ferredoxin in the metabolism of molecular hydrogen. | valentine, r. c. (university of illinois, urbana) and r. s. wolfe. role of ferredoxin in the metabolism of molecular hydrogen. j. bacteriol. 85:1114-1120. 1963.-the metabolism of molecular hydrogen by clostridium pasteurianum, micrococcus lactilyticus (veillonella alcalescens), and several other anaerobic bacteria was studied. oxidation of hydrogen, using several electron-accepting substrates including triphosphopyridine nucleotide, uric acid, xanthine, nitrite, and hydroxylamine, required ferre ... | 1963 | 14044002 |
| metabolic role of the br factor in butyribacterium rettgeri. | kline, leo (university of california, berkeley), l. pine, and h. a. barker. metabolic role of the br factor in butyribacterium rettgeri. j. bacteriol. 85:967-975. 1963.-the br factor, which is replaceable by lipoic acid, was shown to be required for the decomposition of lactate by butyribacterium rettgeri. since the factor was not required for the fermentation of pyruvate or glucose by this organism, the results indicate that br factor is essential only for some reaction involved in the oxidatio ... | 1963 | 14044025 |
| [on the lecithin-vitellin reaction in nonsporiferous bacterial species]. | | 1963 | 14078222 |
| the role of the anaerobic bacteria, with particular reference to the virulence of clostridium perfringens. | | 1964 | 14146376 |
| [anaerobic pathogens in pediatrics]. | | 1963 | 14165235 |
| internal structures of a eubacterium sp. demonstrated by the negative staining technique. | bladen, howard a. (national institute of dental research, bethesda, md.), marie u. nylen, and robert j. fitzgerald. internal structures of a eubacterium sp. demonstrated by the negative staining technique. j. bacteriol. 88:763-770. 1964.-thin sections as well as negatively stained whole cells of a eubacterium sp. isolated from the rat cecum were examined in a siemens elmiskop i electron microscope. the cell wall usually appeared in thin sections as a single dense layer about 130 a thick; however ... | 1964 | 14208516 |
| biohydrogenation of unsaturated fatty acids by rumen bacteria. | polan, c. e. (north carolina state of the university of north carolina, raleigh), j. j. mcneill, and s. b. tove. biohydrogenation of unsaturated fatty acids by rumen bacteria. j. bacteriol. 88:1056-1064. 1964.-a simple, rapid, specific assay for the biohydrogenation of unsaturated fatty acids was developed. with this assay, it was shown that washed suspensions of mixed rumen bacteria hydrogenate linoleic and oleic acids. butyrivibrio fibrisolvens, a common rumen bacterium, is capable of hydrogen ... | 1964 | 14219019 |
| lactate-degrading system in butyribacterium rettgeri subject to glucose repression. | wittenberger, charles l. (national institute of dental research, u.s. public health service, bethesda, md.), and ann s. haaf. lactate-degrading system in butyribacterium rettgeri subject to glucose repression. j. bacteriol. 88:896-903. 1964.-the ability of butyribacterium rettgeri to utilize lactate as the main energy source for growth requires the formation of a lactate-degrading system. the precise nature of this system is unknown, but preliminary evidence suggests that cellular acquisition of ... | 1964 | 14219052 |
| glucose dehydrogenase of bacterium anitratum: an enzyme with a novel prosthetic group. | | 1964 | 14257587 |
| [the behavior of the latex slide test, c-reactive proteins and diphenylamine reaction in rheumatic diseases]. | | 1960 | 14401559 |
| cx-reactive protein response in rabbits during immunization with foreign proteins. | | 1960 | 14402539 |
| [the significance of determination of c-reactive proteins in patients with acute myocardial infarct]. | | 1960 | 14404621 |
| [c-reactive proteins in the differential diagnosis between myocardial infarct and stenocardia]. | | 1960 | 14404622 |
| [studies on the behavior of c-reactive protein in dermatological symptom complexes]. | | 1959 | 14405269 |
| c reactive protein in bacterial meningitis. | | 1959 | 14406768 |
| [study of the behavior of c-reactive protein and fibrinogen in coronary diseases]. | | 1960 | 14407506 |
| [significance of the determination of c-reactive proteins in inflammatory eye diseases]. | | 1959 | 14413391 |
| [new biologic tests in coronary disease]. | | 1959 | 14413458 |
| [the c-reactive protein in hansen's disease]. | | 1960 | 14414034 |
| [the behavior of c-reactive protein in myocardial infarct. a comparison with transaminase, blood sedimentation and leukocyte count]. | | 1960 | 14414488 |
| [monaldi's endocavitary aspiration (e.a.) and its repercussions on the humoral picture of the body studies by the c-reactive protein test (crp)]. | | 1959 | 14418130 |
| [c-reactive protein in the picture of tonsillar pathology. iii. relation between leukometric variations and crp levels following tonsillar stimulation]. | | 1960 | 14421162 |
| [the c-reactive protein in the picture of tonsillar pathology. ii. study of quantitative variations induced by tonsillar stimulation]. | | 1959 | 14421168 |
| [study of the c-reactive protein and of glutamic oxalacetic and pyruvic transaminases in surgical practice]. | | 1960 | 14423520 |
| the c-reactive protein in the serum of lepromatous case in lepra reaction. | | 1960 | 14423731 |
| ["c-reactive protein" in leprous infection]. | | 1959 | 14423732 |
| [antibody formation induced by dextran and c-reactive protein]. | | 1959 | 14423997 |
| [relations between c-reactive protein, erythrocyte sedimentation and colloid lability tests]. | | 1960 | 14425508 |
| [findings and remarks on the clinical significance of the c-reactive protein]. | | 1960 | 14428260 |
| [c-reactive protein in infections of the urinary tract]. | | 1960 | 14432124 |
| [c-reactive protein test]. | | 1959 | 14433076 |
| [c-reactive protein test]. | | 1959 | 14433077 |
| [the behavior of c-reactive protein in arteriosclerotic vascular diseases]. | | 1960 | 14434238 |
| [observation on the presence and significance of c-reactive protein in the postoperative period]. | | 1960 | 14434735 |
| [the c-reactive protein in the pneumoconiosis of coal miners]. | | 1959 | 14434892 |
| [behavior of creactive protein, chondroitin sulfuric acid and hyaluronic acid in human articular fluid of the knee]. | | 1959 | 14435477 |
| [on diagnostic possibility of the "active" phase of rheumatic disease in the light of clinical, biochemical and histopathological studies. report i. behavior of aldolase, transaminase and of certain other laboratory and clinical indices in patients subjected to surgical interventions on the bicuspid valve]. | | 1960 | 14435799 |
| [c-reactive protein in myocardia infarct]. | | 1959 | 14438628 |
| c-reactive proteins in the sera of patients with leprosy. | | 1959 | 14439145 |
| [on the behavior of c-reactive protein and lysozyme activity in pelvic inflammations]. | | 1960 | 14441558 |
| [on the appearance of the c-reactive protein after the administration of dextran]. | | 1959 | 14441688 |
| [gamma-x-globulin, an immunoelectrophoretic demonstrable gamma-globulin-component with relations to c-reactive protein]. | | 1960 | 14443811 |
| antibiotic activity of myxobacteria in relation to their bacteriolytic capacity. | norén, börge (university of wisconsin, madison) and kenneth b. raper. antibiotic activity of myxobacteria in relation to their bacteriolytic capacity. j. bacteriol. 84:157-162. 1962-myxococcus virescens, m. fulvus, m. stipitatus, m. lacteus, chondrococcus blasticus, and chondromyces crocatus were tested for antibacterial activity, and all were found to secrete products that inhibited the growth of gram-positive eubacteria. the amount of inhibition varied with the myxobacterium employed and with ... | 1962 | 14480333 |
| novel polar lipids of halophilic eubacterium planococcus h8 and archaeon haloferax volcanii. | as part of a study to identify novel lipids with immune adjuvant activity, a structural comparison was made between the polar lipids from two halophiles, an archaeon haloferax volcanii and a eubacterium planococcus h8. h. volcanii polar lipid extracts consisted of 44% archaetidylglycerol methylphosphate, 35% archaetidylglycerol, 4.7% of archaeal cardiolipin, 2.5% archaetidic acid, and 14% sulfated glycolipids 1 and 2. nuclear magnetic resonance (nmr) and fast atom bombardment mass spectrometry ( ... | 2003 | 14499737 |
| chlamydia pneumoniae activates epithelial cell proliferation via nf-kappab and the glucocorticoid receptor. | chlamydia pneumoniae is an obligate intracellular eubacterium and a common cause of acute and chronic respiratory tract infections. this study was designed to show the effect of c. pneumoniae on transcription factor activation in epithelial cells. the activation of transcription factors by c. pneumoniae was determined in human epithelial cell lines (hl and calu3) by electrophoretic dna mobility shift assay, western blotting, and luciferase reporter gene assay. the activation of transcription fac ... | 2003 | 14500503 |
| cspb and cspl, thermostable cold-shock proteins from thermotoga maritima. | cold-shock proteins (csps) are important for cellular adaptation to low temperature. csps help cells adapt to low-temperature growth through their rna-binding and nucleic acid melting abilities, which lead to anti-termination of transcription. | 2003 | 14531859 |
| a possible role of cellulose-binding protein a (cbpa) in the adhesion of eubacterium cellulosolvens 5 to cellulose. | the cellulose-binding protein a (cbpa) of eubacterium cellulosolvens 5 is a modular enzyme comprised of a catalytic domain, a cellulose-binding domain and a cell wall-binding domain. cellobiose-grown cells changed their adhesion ability to cellulose depending on the growth phase. on the other hand, carboxymethyl cellulose (cmc)-grown cells bound to cellulose regardless of their growth phase. the distribution of cbpa in the culture supernatant and cell fractions changed depending on the carbon so ... | 2003 | 14581993 |
| [thermotoga neapolitana gene clusters participating in degradation of starch and maltodextins: molecular structure of the locus]. | a 5451-bp genome fragment of the hyperthermophilic anaerobic eubacterium thermotoga neapolitana has been cloned and sequenced. the fragment contains one truncated and three complete open reading frames highly homologous to the starch/maltodextrin utilization gene cluster from thermotoga maritima whose genome sequence is known. the incomplete product of the first frame is highly homologous to malg, the e. coli protein of starch and maltodextrin transport. the product of the second frame, aglb, is ... | 2003 | 14593916 |
| use of the microorganism bacillus stearothermophilus as a model to evaluate toxicity of the lipophilic environmental pollutant endosulfan. | microorganisms are very powerful tools for the supply of information about the toxic effects of lipophilic compounds, since an impairment of cell growth usually occurs as a result of perturbations related, in most cases, with the partition of toxicants in membranes. the thermophilic eubacterium bacillus stearothermophilus has been used as a model system to identify alpha- and beta-endosulfan interactions with the membrane possibly related with the insecticide toxicity. two approaches have been p ... | 2003 | 14599450 |
| molecular mechanisms of the metabolite 4-hydroxytamoxifen of the anticancer drug tamoxifen: use of a model microorganism. | a strain of the thermophilic eubacterium bacillus stearothermophilus was used as a model system to identify membrane mediated cytotoxic effects of 4-hydroxytamoxifen, following previous studies with tamoxifen. with this experimental approach we attempted to further clarify tamoxifen and 4-hydroxytamoxifen membrane interactions often evoked as responsible for their multiple cellular effects. bacterial growth and the oxygen consumption rate provided quantitative data of the cytotoxic action of hyd ... | 2003 | 14599455 |
| trigger factor from thermus thermophilus is a zn2+-dependent chaperone. | the ribosome-associated chaperone trigger factor (tf) of escherichia coli interacts with a variety of newly synthesized polypeptides to assist their correct folding. here, we report that the tf of thermophilic eubacterium, thermus thermophilus, arrested spontaneous folding of green fluorescent protein by forming a 1:1 binary complex. the complex was isolable by gel-filtration but was shown to be dynamic because green fluorescent protein was released by alpha-casein in large excess. unexpectedly, ... | 2004 | 14602709 |
| effect of dental films containing amoxycillin and metronidazole on periodontal pathogens: microbiological response. | biodegradable dental films containing a combination of amoxycillin and metronidazole were prepared by a dispersion method. the aim of the present study was to determine the antimicrobial activity of this formulation against the pathogens which are commonly implicated in periodontal infections. films showed sustained release in vitro for a period of 16 days. in situ release studies carried out using fresh bovine buccal mucosa in a flow through cell, showed that drug concentrations were maintained ... | 2003 | 14609283 |
| identification and characterization of the cytoplasmic tungstate/molybdate-binding protein (mop) from eubacterium acidaminophilum. | the mop gene, encoding the molybdate-binding protein from eubacterium acidaminophilum, was cloned using clostridium pasteurianum mopi as a probe for heterologous hybridization. mop encodes a 69-amino-acid protein ( m(r) 7,328) with high sequence similarities to members of the molbindin protein family, which have been implicated in molybdenum storage and homeostasis. northern blot analysis showed three mrna transcripts (1.0, 1.6, and 2.6 kb) for mop. this result was obtained independent of the av ... | 2004 | 14624334 |
| pseudoramibacter alactolyticus in primary endodontic infections. | a nested polymerase chain reaction (pcr)-based method was used to directly survey samples taken from primary endodontic infections for the occurrence of pseudoramibacter alactolyticus. identification by nested pcr was performed in root-canal samples from teeth associated with asymptomatic periradicular lesions or acute apical periodontitis, and in pus samples from acute periradicular abscesses. dna was extracted from the samples and initially amplified using universal 16s rdna primers. a second ... | 2003 | 14651280 |
| discovery and characterization of a thermostable bacteriophage rna ligase homologous to t4 rna ligase 1. | thermophilic viruses represent a novel source of genetic material and enzymes with great potential for use in biotechnology. we have isolated a number of thermophilic viruses from geothermal areas in iceland, and by combining high throughput genome sequencing and state of the art bioinformatics we have identified a number of genes with potential use in biotechnology. we have also demonstrated the existence of thermostable counterparts of previously known bacteriophage enzymes. here we describe a ... | 2003 | 14654700 |
| idiomarina loihiensis sp. nov., a halophilic gamma-proteobacterium from the lō'ihi submarine volcano, hawai'i. | during an investigation of bacterial diversity at hydrothermal vents on the lō'ihi seamount, hawai'i, a novel bacterium (designated l2-tr(t)) was cultivated, which shares 99.9 % 16s rrna gene sequence similarity over 1415 nt with an uncultured eubacterium from sediment at a depth of 11 000 m in the mariana trench. the nearest cultivated neighbour of l2-tr(t), however, is idiomarina abyssalis kmm 227(t), with which it shares 98.9 % 16s rrna sequence similarity. l2-tr(t) differed from i. abyssalis ... | 2003 | 14657116 |
| the ro autoantigen binds misfolded u2 small nuclear rnas and assists mammalian cell survival after uv irradiation. | the ro 60 kda autoantigen, an rna binding protein, is a major target of the immune response in patients with systemic lupus erythematosus. as mice lacking ro develop a lupus-like syndrome, ro may be important for preventing autoimmunity. however, the cellular function of ro, which binds small cytoplasmic rnas of unknown function called y rnas, has been enigmatic. ro has been proposed to function in 5s rrna quality control based on experiments in xenopus laevis oocytes, and a ro ortholog enhances ... | 2003 | 14680639 |
| tungsten-containing aldehyde oxidoreductase of eubacterium acidaminophilum. | aldehyde oxidoreductase of eubacterium acidaminophilum was purified to homogeneity under strict anaerobic conditions using a four-step procedure. the purified enzyme was present as a monomer with an apparent molecular mass of 67 kda and contained 6.0 +/- 0.1 iron, 1.1 +/- 0.2 tungsten, about 0.6 mol pterin cofactor and zinc, but no molybdenum. the enzyme activity was induced if a molar excess of electron donors, such as serine and/or formate, were supplied in the growth medium compared to readil ... | 2004 | 14686934 |
| a cross-genomic approach for systematic mapping of phenotypic traits to genes. | we present a computational method for de novo identification of gene function using only cross-organismal distribution of phenotypic traits. our approach assumes that proteins necessary for a set of phenotypic traits are preferentially conserved among organisms that share those traits. this method combines organism-to-phenotype associations,along with phylogenetic profiles,to identify proteins that have high propensities for the query phenotype; it does not require the use of any functional anno ... | 2004 | 14707173 |
| stereochemical course of the generation of 3-mercaptohexanal and 3-mercaptohexanol by beta-lyase-catalyzed cleavage of cysteine conjugates. | the product resulting from the reaction between e-2-hexenal and l-cysteine was shown to be a diastereoisomeric mixture of 2-(2-s-l-cysteinylpentyl)-1,3-thiazolidine-4-carboxylic acid 1. treatment of the conjugate with two sources of cysteine-s-conjugate beta-lyase (tryptophanase from e. coli and a crude enzyme extract prepared from eubacterium limosum) resulted in the formation of 3-mercaptohexanal. the reaction proceeded with a slight preference for the (s)-configured product, however, with low ... | 2004 | 14709022 |
| polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. | in this study, we aimed to investigate the occurrence of several microbial species in cases of failed endodontic therapy by means of the polymerase chain reaction (pcr). study design root canal samples were taken from 22 root-filled teeth with persistent periradicular lesions selected for re-treatment. dna was extracted from the samples and analyzed for the presence of 19 microbial taxa by using the polymerase chain reaction. | 2004 | 14716262 |
| genomic investigation of the system for selenocysteine incorporation in the bacterial domain. | the elucidation of nearly 100 bacterial genomes has made it possible to categorize them into two groups, according to the presence or absence of a selenocysteine (sec) trna. in the group with the trna, a sec incorporation system like that of escherichia coli would be expected. however, for the other group, the following question has been left unsolved. is it reasonable to assume that bacteria without the trna lack the entire sec system, and do such bacteria exist commonly? to explore it experime ... | 2004 | 14732487 |
| bacillus odysseyi sp. nov., a round-spore-forming bacillus isolated from the mars odyssey spacecraft. | a round-spore-forming bacillus species that produces an exosporium was isolated from the surface of the mars odyssey spacecraft. this novel species has been characterized on the basis of phenotypic traits, 16s rdna sequence analysis and dna-dna hybridization. according to the results of these analyses, this strain belongs to the genus bacillus and is a gram-positive, aerobic, rod-shaped, endospore-forming eubacterium. ultrathin sections of the spores showed the presence of an exosporium, spore c ... | 2004 | 14742480 |
| bacteremia following surgical dental extraction with an emphasis on anaerobic strains. | our aim was to investigate bacteremia caused by surgical extraction of partly erupted mandibular third molars. from 16 young adults, bacterial samples were taken from the third-molar pericoronal pocket and post-operatively from the extraction socket, and blood samples were drawn from the ante-cubital vein up to 30 min after surgery. of the subjects, 88% had detectable bacteremia-50% 1 min after the incision, 44% immediately after extraction. the respective percentages at 10, 15, and 30 min were ... | 2004 | 14742658 |
| relationship between periodontal pocket sulfide levels and subgingival species. | many species implicated in the pathogenesis of periodontal disease produce volatile sulfur compounds (vsc). this investigation examined the relationship between levels of sulfide and subgingival bacterial species in the same periodontal pockets. | 2003 | 14761124 |
| microbiological examination of infected dental root canals. | the aim of this study was to investigate the root canal microbiota of primary and secondary root-infected canals and the association of constituent species with specific endodontic signs and symptoms. | 2004 | 14871344 |
| the role of colonic microbiota in lactose intolerance. | in a previous study we observed a clear difference in lactose intolerance symptoms after a 25-g lactose load in two groups of persons with lactase nonpersistence and similar small intestinal lactase activity. from this observation we hypothesized a colon resistance factor. to identify this factor, the microbial composition of fecal samples of the two lactose intolerant groups (one with mild symptoms, n = 16, and one with diarrhea-predominant symptoms, n = 11) was compared using the fluorescent i ... | 2004 | 14992439 |
| characterization of a thermostable l-arabinose (d-galactose) isomerase from the hyperthermophilic eubacterium thermotoga maritima. | the araa gene encoding l-arabinose isomerase (ai) from the hyperthermophilic bacterium thermotoga maritima was cloned and overexpressed in escherichia coli as a fusion protein containing a c-terminal hexahistidine sequence. this gene encodes a 497-amino-acid protein with a calculated molecular weight of 56,658. the recombinant enzyme was purified to homogeneity by heat precipitation followed by ni(2+) affinity chromatography. the native enzyme was estimated by gel filtration chromatography to be ... | 2004 | 15006759 |
| [the prevalence of actinobaculum suis in boars of breeding herds in the omsk region (russian federation) by indirect immunofluorescence technique]. | actinobaculum suis (corynebacterium suis, eubacterium suis, actinomyces suis) was detected in the preputial diverticulum of 64,8% of 162 boars investigated in 8 districts of the region omsk (russian federation) by indirect immunofluorescent technique. until yet no informations were available about the prevalence of actinobaculum (a.) suis in swine herds of the russian federation. the study shows that a. suis, as a main aetiological factor of cystitis and pyelonephritis in sows, is widely spread ... | 2004 | 15032264 |
| malic enzymes of trichomonas vaginalis: two enzyme families, two distinct origins. | the cytosolic malic enzyme of the amitochondriate protist trichomonas vaginalis was purified to homogeneity and characterized. the corresponding gene was sequenced and compared with its hydrogenosomal homologue from the same organism. the enzymes were found to differ in coenzyme specificity, molecular mass and physiological role. the cytosolic malic enzyme is a dimer consisting of two 42-kda subunits with strict specificity for nicotinamide adenine dinucleotide phosphate (nadp(+)), and has a pre ... | 2004 | 15033531 |
| [anaerobes analysis in 80 cases with ora maxillofacial infections] | objective:to define the infection types of 80 cases of ora-maxillofacial infection and investigate its anaerobes' distribution and the role of the anaerobes' metabolic products in their identification.methods:we isolated and cultured anaerobes from the purulent specimen and applied the gasliquid chromatograph (glc) technique to analyze the anaerobes' metabolic products to define their genera and species in the meantime.results:bacteria were isolated from all of the purulent specimen.isolation ra ... | 1999 | 15048214 |
| molecular analysis of the xylfgh operon, coding for xylose abc transport, in thermoanaerobacter ethanolicus. | a xylose abc (atp-binding cassette) transport operon, xylfgh, was cloned from thermoanaerobacter ethanolicus, a thermophilic ethanol-producing eubacterium. the cistrons code for a periplasmic d-xylose-binding protein (xylf, partial sequence of 250 amino acids), atp-binding protein (xylg, 505 amino acids), and integral membrane protein (xylh, 388 amino acids). these results, together with previous work, indicate that duplicate copies of both xylf and xylh are present in the t. ethanolicus chromos ... | 2004 | 15057456 |
| functional analysis of the small subunit of the putative homoaconitase from pyrococcus horikoshii in the thermus lysine biosynthetic pathway. | an in vivo disruption-integration vector system for thermus thermophilus was developed and used for the functional analysis of an evolutionary-related archaeal protein for lysine biosynthesis. in contrast to fungal one, the putative homoaconitase of t. thermophilus consists of two subunits and catalyzes the second and third steps of lysine biosynthesis. orfs from hyperthermophilic archaeon pyrococcus horikoshii, ph1726 and ph1724, share a high degree of amino acid identity with the t. thermophil ... | 2004 | 15063502 |
| thermodynamic analysis of the unfolding and stability of the dimeric dna-binding protein hu from the hyperthermophilic eubacterium thermotoga maritima and its e34d mutant. | we have studied the stability of the histone-like, dna-binding protein hu from the hyperthermophilic eubacterium thermotoga maritima and its e34d mutant by differential scanning microcalorimetry and cd under acidic conditions at various concentrations within the range of 2-225 micro m of monomer. the thermal unfolding of both proteins is highly reversible and clearly follows a two-state dissociation/unfolding model from the folded, dimeric state to the unfolded, monomeric one. the unfolding enth ... | 2004 | 15066175 |
| reduction in diversity of the colonic mucosa associated bacterial microflora in patients with active inflammatory bowel disease. | the intestinal bacterial microflora plays an important role in the aetiology of inflammatory bowel disease (ibd). as most of the colonic bacteria cannot be identified by culture techniques, genomic technology can be used for analysis of the composition of the microflora. | 2004 | 15082587 |
| comparison of type 1 and type 2 cytokine production by mononuclear cells cultured with streptococcus mutans and selected other caries bacteria. | a feature of pulpal immune responses is the predominance of type 1 cytokine mrna under shallow caries and a mixed (type 1/type 2) profile under deep caries. these results prompted an examination of the cytokine profiles induced by bacteria in shallow caries (streptococcus mutans and actinomyces viscosus) and deep caries (lactobacillus casei, pseudoramibacter alactolyticus, and prevotella intermedia). all isolates induced interferon-gamma and interleukin-10, whereas interleukin-4 and interleukin- ... | 2004 | 15107645 |
| first bacterial chalcone isomerase isolated from eubacterium ramulus. | the human fecal anaerobe eubacterium ramulus is capable of degrading various flavonoids, including the flavone naringenin. the first step in the proposed degradation pathway is the isomerization of naringenin to the corresponding chalcone. cell-free extracts of e. ramulus displayed chalcone isomerase activity. the enzyme from e. ramulus was purified to homogeneity. its apparent molecular mass was estimated to be 136 and 129 kda according to gel filtration and native polyacrylamide gel electropho ... | 2004 | 15127184 |
| the bacterial cytoskeleton and its putative role in membrane vesicle formation observed in a gram-positive bacterium producing starch-degrading enzymes. | bacteria may possess various kinds of cytoskeleton. in general, bacterial cytoskeletons may play a role in the control and preservation of the cell shape. such functions become especially evident when the bacteria do not possess a true wall and are nevertheless elongated (e.g. mycoplasma spp.) or under extreme cultivation conditions whereby loss of the entire bacterial cell wall takes place. bacterial cytoskeletons may control and preserve the cell shape only if a number of preconditions are ful ... | 2003 | 15153765 |
| in vitro activities of the new semisynthetic glycopeptide telavancin (td-6424), vancomycin, daptomycin, linezolid, and four comparator agents against anaerobic gram-positive species and corynebacterium spp. | telavancin is a new semisynthetic glycopeptide anti-infective with multiple mechanisms of action, including inhibition of bacterial membrane phospholipid synthesis and inhibition of bacterial cell wall synthesis. we determined the in vitro activities of telavancin, vancomycin, daptomycin, linezolid, quinupristin-dalfopristin, imipenem, piperacillin-tazobactam, and ampicillin against 268 clinical isolates of anaerobic gram-positive organisms and 31 corynebacterium strains using agar dilution meth ... | 2004 | 15155214 |
| contribution of acetate to butyrate formation by human faecal bacteria. | acetate is normally regarded as an endproduct of anaerobic fermentation, but butyrate-producing bacteria found in the human colon can be net utilisers of acetate. the butyrate formed provides a fuel for epithelial cells of the large intestine and influences colonic health. [1-(13)c]acetate was used to investigate the contribution of exogenous acetate to butyrate formation. faecalibacterium prausnitzii and roseburia spp. grown in the presence of 60 mm-acetate and 10 mm-glucose derived 85-90 % but ... | 2004 | 15182395 |
| pcr detection and 16s rrna sequence-based phylogeny of a novel propionibacterium acidipropionici applicable for enhanced fermentation of high moisture corn. | the aims of this study were to develop a sensitive and more rapid detection of propionibacterium acidipropionici dh42 in silage and rumen fluid samples, and to explore its 16s rrna sequence-based phylogeny. | 2004 | 15186440 |
| gram-positive anaerobic bacilli in human periodontal disease. | the uncertain taxonomy of oral anaerobic gram-positive bacilli and their generally slow growing nature has limited the understanding of their role in periodontal disease. the current objective was to design and use species-specific oligonucleotide probes to investigate the relationship of selected gram-positive anaerobic bacilli to periodontal disease. | 2004 | 15206913 |
| enhanced biohydrogen production from sewage sludge with alkaline pretreatment. | batch tests were carried out to analyze influences of the alkaline pretreatment and initial ph value on biohydrogen production from sewage sludge. experimental results of the impact of different initial ph on biohydrogen production showed that both the maximal hydrogen yield occurred and that no methane was detected in the tests of at the initial ph of 11.0. the final ph decreased at the initial ph of 7.0-12.5 but increased atthe initial ph of 3.0-6.0, probably due to the combination of solubili ... | 2004 | 15224755 |
| detection of cellulolytic bacteria from the human colon. | the main representatives of bacteria in the human colon were investigated by specific pcr and denaturing gradient gel electrophoresis (dgge). prevalent in both cases were species of bifidobacterium, clostridium, bacteroides, faecalibacterium and eubacterium. simultaneously, cellulolytic bacteria were isolated from the human feces. the largest proportion was represented by ruminococcus-like isolates. their presence was confirmed both by pcr and dgge methods; the latter one was able to give more c ... | 2004 | 15227792 |
| identification of bacterial populations in dairy wastewaters by use of 16s rrna gene sequences and other genetic markers. | hydraulic flush waste removal systems coupled to solid/liquid separators and circulated treatment lagoons are commonly utilized to manage the large amounts of animal waste produced on high-intensity dairy farms. although these systems are common, little is known about the microbial populations that inhabit them or how they change as they traverse the system. using culture-based and non-culture-based methods, we characterized the microbial community structure of manure, water from the separator p ... | 2004 | 15240310 |
| dna microarray analysis of predominant human intestinal bacteria in fecal samples. | a microarray method was developed for the detection of 40 bacterial species reported in the literature to be predominant in the human gastrointestinal tract. the 40 species include seven species each of bacteroides and clostridium, six species of ruminococcus, five species of bifidobacterium, four species of eubacterium, two species each of fusobacterium, lactobacillus and enterococcus, and single species each of collinsella, eggerthella, escherichia, faecalibacterium and finegoldia. three 40-me ... | 2004 | 15271382 |