| effects of local anesthetics on bacterial cells. | the membrane effects of chlorpromazine, nupercain, tetracain, and procain were studied using bacillus cereus, b. megaterium, b. subtilis, and streptococcus faecalis, protoplasts from s. faecalis, and isolated membranes from b. subtilis. chlorpromazin, nupercain, and tetracain produced characteristic micromorphological alterations after treatment for 5 to 30 min at ph 7.0 and 20 degrees c; the membrane staining pattern changed from asymmetric to symmetric, complex mesosome-like structures appeare ... | 1979 | 104970 |
| ultrastructure and cytochemical localization of laccase in two strains of leptosphaerulina briosiana (pollaci) graham and luttrell. | substrate specificity tests were used to identify the presence of laccase in two strains of leptosphaerulina briosiana (poll.) graham and luttrell, an ascomycete which causes leaf spot in alfalfa. cytochemical localization of monophenol monooxygenase (laccase) as well as the ultrastructures of the two strains were investigated. laccase was observed in the outer layers of the cell walls of both strains. the ultrastructures of vegetative hyphae of both strains were typical of those found in most a ... | 1979 | 104971 |
| plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pe194. | a plasmid, pe194, obtained from staphylococcus aureus confers resistance to macrolide, lincosamide, and streptogramin type b ("mls") antibiotics. for full expression, the resistance phenotype requires a period of induction by subinhibitory concentrations of erythromycin. a copy number in the range of 10 to 25 copies per cell is maintained during cultivation at 32 degrees c. it is possible to transfer pe194 to bacillus subtilis by transformation. in b. subtilis, the plasmid is maintained at a cop ... | 1979 | 104975 |
| effect of light on the attachment of cyanophage as-1 to anacystis nidulans. | the effect of illumination on the extent and kinetics of the adsorption of cyanophage as-1 to the blue-green alga (cyanobacterium) anacystis nidulans was studied by using 32p-labeled phage. the initial rate of adsorption was not significantly affected by light. however, at na+ levels used ordinarily to culture the alga ([na+] = 11.7 mm), the total amount of phage adsorbed was doubled in the illuminated cultures, as compared with the dark-grown ones, over a wide range of multiplicities of infecti ... | 1979 | 104977 |
| localization of membrane-derived oligosaccharides in the outer envelope of escherichia coli and their occurrence in other gram-negative bacteria. | the glucose-containing, membrane-derived oligosaccharides of escherichia coli are localized in the external envelope of that organism, most probably in the periplasmic space. the membrane-derived oligosaccharides appear to be generally occurring cell constituents of gram-negative (but not gram-positive) bacteria. | 1979 | 104978 |
| x-ray analysis of ferredoxin from spirulina platensis. ii. chelate structure of active center. | a chloroplast-type ferredoxin from spirulina platenis crystallized in an orthorhombic system, space group c2221, with cell dimensions a=62.32, b=28.51, and c=108.08 a. the electron density map at 2.8 a resolution was prepared by using the best phase angles determined by the single isomorphous replacement method coupled with the anomalous dispersion method. the chelating structure of the acitve center was revealed as follows. of the six cysteinyl residues in the molecule, cys 41, cys 4k, cys 49, ... | 1978 | 104982 |
| biochemical properties of a new flexuous bacteriocin, pyocin f1, produced by pseudomonas aeruginosa. | pseudomonas aeruginosa strain p15 produces not only pyocin r1 and phage ps10, but also a substance having a flexuous rod structure, the nature of which is so far unknown. a variant strain (p15--40) was obtained which produced these flexuous particles more effectively than the original strain, and the particles were purified to homogeneity and investigated. several strains of p. aeruginosa were found to be killed by the particles. it was concluded that the flexuous rod-like particles are not rela ... | 1979 | 104991 |
| the polysaccharides from heterocyst and spore envelopes of a blue-green alga. structure of the basic repeating unit. | the polysaccharides from the envelopes of heterocysts and spores of anabaena cylindrica consist of repeating units containing 1 mannosyl and 3 glucosyl residues, all linked by beta(1 yields 3) glycosidic bonds, with glycosidic bonds, with glucose, xylose, galactose, and mannose present in side branches. degradation of the polysaccharides with specific glycosidases has permitted identification of the linkages to almost all of the branches. when the polysaccharides, from which all but two types of ... | 1979 | 104995 |
| the primary structure of lactobacillus casei thymidylate synthetase. i. the isolation of cyanogen bromide peptides 1 through 5 and the complete amino acid sequence of cnbr 1, 2, 3, and 5. | thymidylate synthetase from lactobacillus casei was s-carboxymethylated and degraded by treatment with cyanogen bromide. although the protein contains 6 methionine residues, only 5 cyanogen bromide peptides were obtained due to the presence of 1 methionine on the nh2 terminus and another adjacent to a threonine residue which was resistant to cleavage. the peptides were isolated by differential extraction, first with ammonium acetate, then pyridine acetate, and finally the residue was solubilized ... | 1979 | 105003 |
| the primary structure of lactobacillus casei thymidylate synthetase. iii. the use of 2-(2-nitrophenylsulfenyl)-3-methyl-3-bromoindolenine and limited tryptic peptides to establish the complete amino acid sequence of the enzyme. | | 1979 | 105005 |
| rapid, sensitive and specific electron capture-gas chromatographic method for the quantitation of 6-chloro-2-(1-piperazinyl) pyrazine in biological fluids. | a highly specific and sensitive gas chromatographic method for the determination of 6-chloro-2-(1-piperazinyl)pyrazine (mk-212), a central serotonin-like agent, in biological fluids is described. mk-212 and a related internal standard are extracted into benzene from an alkaline solution, back-extracted into acid and then re-extracted into benzene at an alkaline ph. the amines are converted to the trifluoroacetyl derivatives (characterized by gas-liquid chromatography-mass spectrometry), chromato ... | 1979 | 105011 |
| assessment of the efficacy of interventions to limit ischemic injury by direct measurement of intramural carbon dioxide tension after coronary artery occlusion in the dog. | although numerous interventions have been shown to exert a salutary effect on the ischemic myocardium, the severity of ischemia generally has been measured by indirect techniques. in the present investigation the effect of ischemia on intramural carbon dioxide tension (pmco(2)) was measured directly in the open-chest, anesthetized dog with a mass spectrometer during repetitive 10-min coronary artery occlusions separated by 45-min periods of reflow; simultaneously, regional myocardial blood flow ... | 1979 | 105016 |
| human dentition and experimental animals. | a review of the recent literature concerning enamel maturation patterns following cessation of protein matrix secretion confirms that developing rodent enamel exhibits an overall mineral distribution different from that of developing enamel in man, monkey, dog, and swine. this seems to be a result of structural differences of the enamel as the pattern of crystal growth appears similar in these species. whether the differences in enamel structure are reflected in significant differences in cellul ... | 1979 | 105027 |
| the specificity of the anti-haemagglutinin antibody response induced in man by inactivated influenza vaccines and by natural infection. | the anti-haemagglutinin antibody response in adult human volunteers to inactivated whole virus or tween ether split influenza a/victoria/75 (h3n2) and a/scotland/74 (h3n2) virus vaccines was investigated using antibody absorption and single-radial-haemolysis (srh) techniques. the concentrations of haemagglutinin (ha), nucleoprotein (np) and matrix (m) antigens measured by single radial diffusion (srd) and rocket immunoelectrophoresis were similar for both the whole virus and split vaccines. whol ... | 1979 | 105040 |
| early steps in specific tumor cell lysis by sensitized mouse t lymphocytes. iii. resolution of two distinct roles for calcium in the cytolytic process. | | 1979 | 105046 |
| immunological cross-reactivity between a glycoprotein isolated from trichophyton mentagrophytes and human isoantigen a. | a glycoprotein isolated from the cell wall of trichophyton mentagrophytes was assessed for its cross-reaction with human blood group isoantigens. rabbit antiglycoprotein antibodies agglutinated human erythrocytes of blood groups a1 and a2, and precipitated blood group substance a in agarose gels. erythrocytes of blood group b were only slightly agglutinated, and o(rho+) and o(rho-) erythrocytes were not. additionally, the glycoprotein was shown to specifically inhibit isoagglutination of erythro ... | 1979 | 105052 |
| effect of serum on gram-positive cocci grown in the presence of penicillin. | strains of staphylococcus aureus, streptococcus faecalis, streptococcus bovis, and a strain of streptococcus that produces filaments were grown on agar containing penicillin at concentrations of one-half to 1/10th the minimal inhibitory concentration. these penicillin-exposed organisms as well as untreated control organisms were incubated with human serum of plasma. both serum and plasma produced a remarkable bactericidal effect on the filament-forming streptococcus grown in the presence of peni ... | 1978 | 105057 |
| an animal model for the study of immunopathogenesis of periapical lesions. | | 1978 | 105072 |
| interaction between cytochalasin b-treated malarial parasites and erythrocytes. attachment and junction formation. | we have previously demonstrated that invasion of erythrocytes (rbcs) by malaria merozoites follows a sequence: recognition and attachment in an apical orientation associated with widespread deformation of the rbc, junction formation, movement of the junction around the merozoite that brings the merozoite into the invaginated rbc membrane, and sealing of the membrane. in the present paper, we describe a method for blocking invasion at an early stage in the sequence. cytochalasin-treated merozoite ... | 1979 | 105074 |
| implications for adult roles from differential styles of mother-infant bonding: an ethological study. | ethological observations of maternal and infant behaviors of nine vervet monkey pairs (cercopithecus aethiops sabaeus) showed the effects of differential styles of early maternal responsiveness on later infant competence. those infants receiving the least amount of maternal responsiveness and the most time-off the mother in the first 3 months of development were more socially competent at 6 months of age. the results are discussed within current ethological "attachment" theories. the detachment ... | 1979 | 105083 |
| wits versus genes: the continuing battle against infection. | | 1979 | 105149 |
| sarcocystis and related organisms in australian wildlife: i. survey findings in mammals. | | 1978 | 105152 |
| pseudomonas sternotomy wound infection and sternal osteomyelitis. complications after open heart surgery. | pseudomonas aeruginosa sternotomy wound infections occurred in five patients who underwent open heart surgery. the initial isolate in each case was from a mediastinal chest tube routinely cultured on removal. soft-tissue infection developed in two patients, and sternal osteomyelitis developed in three patients. pseudomonas-typing studies showed a correlation between five isolates from chest-tube suction pumps used postoperatively and the wound isolates. analysis of antibiograms of pseudomonas wo ... | 1979 | 105156 |
| leukemia in workers exposed to ethylene oxide. | ethylene oxide, a gaseous sterilant extensively used within health care facilities, is known to be a mutagen in bacteria and in human lymphocytes. the environmental protection agency as well as the national institue of occupational safety and health have recently stipulated certain conditions for the use of ethylene oxide despite the lack of case reports or epidemiologic studies concerning carcinogenicity. we report three cases of leukemia that occurred between 1972 and 1977 in a relatively smal ... | 1979 | 105157 |
| acute leukemia following prolonged cytotoxic agent therapy. | 1. nine patients in whom acute non-lymphoblastic leukemia (anll) developed following prolonged alkylating agent therapy are described. five of the patients received no radiotherapy. the conditions treated were: hodgkin's disease (four patients), primary amyloidosis, primary macroglobulinemia, malignant lymphoma, multiple myeloma, and carcinoma of the tonsil. 2. prior to the advent of chemotherapy, this complication was not observed in large series of patients with lymphoproliferative disorders a ... | 1979 | 105227 |
| construction of a recombinant plasmid composed of b. subtilis leucine genes and a b. subtilis (natto) plasmid: its use as cloning vehicle in b. subtilis 168. | | 1978 | 105237 |
| the relationship between molecular structure and transformation efficiency of some s. aureus plasmids isolated from b. subtilis. | dna preparations of the chloramphenicol resistance determining s. aureus plasmids pc194, pc223, and pub112 can be fractionated by gel electrophoresis into various bands. electronmicroscopic investigations of these various molecular species obtained with pc194 indicated that, depending on the preparations, 70 to 80% of the molecules were monomers, while the rest consisted of various classes of concatemeric and/or interlocked multimers. measurements of the specific transforming activity of the var ... | 1978 | 105241 |
| genetic exchange in bacillus subtilis in soil. | genetically labelled strains of bacillus subtilis have been shown to exchange blocks of linked genes while growing together in soil. after eight days of incubation, 79% of unselected colony-forming units exhibited a phenotype containing markers from both parents; the parental strains were not detected after the first day of incubation. high frequencies of transformation were also obtained by adding genetically labelled deoxyribonucleic acid to single-strain soil cultures. observed linkage of gen ... | 1978 | 105243 |
| [a new concept of the pathogenesis of medial dysplasia (author's transl)]. | a unifying concept may be set out briefly in the following manner: injurious agents (such as abnormal hemodynamic and/or metabolic conditions--"risk factors for the vessel wall") leads to the transformation of contractile (k) smooth muscle cells into the metabolically more active or "modified" (m) variety leads to an increase in the number of intracellular and extracellular lysosomes (matrix lysosomes) leads to medial dysplasia. the vague concept of a "vessel wall weakness" as the cause of aneur ... | 1979 | 105260 |
| the search for a conceptual framework as a philosophic basis for nursing ethics: an examination of code, contract, context, and covenant. | | 1979 | 105323 |
| thyroid function in long term haemofiltration. | ten patients on haemofiltration treatment for between 4 and 20 months have been screened for thyroid function. comparing serum t3 and t4 levels at the start of haemofiltration and 5 to 20 months later, a continuous decrease was detected (t3-ria: 92 +/- 23 ng% to 68 +/- 23 ng%; t4-ria: 4.94 +/- 1.79 microgram% to 3.81 +/- 1.21 microgram%). serum tsh-ria values also declined in mean from 3.48 +/- 0.47 muu/ml, yet two patients showed an increase as expected with a functioning hypothalamic pituitary ... | 1978 | 105354 |
| cell-mediated and humoral immune responses to cartilage antigenic components. | cell-mediated immunity and antibody production to cartilage antigens were studied in rabbits. from day 3 of immunization an inhibition of leucocyte migration was observed in animals immunized with collagen-free fractions of bovine nasal or human rib cartilage. delayed cutaneous hypersensitivity was elicited between 5 and 6 days and the circulating antibodies were demonstrated by passive haemagglutination on day 9. no significant correlation was observed between the antibody production and the ce ... | 1979 | 105405 |
| [effect of polychlorinated biphenyls in the rat: i. -- relation tissue level dose (author's transl)]. | rats were fed with a diet containing phenoclor dp6, a french commercial polychlorinated biphenyl (pcb) mixture, for 30 days. diet pcb levels were 10, 100, 500 and 1000 ppm (wet weight). at 500 ppm, a 50 per cent mortality was found after 8 days, while a 100 per cent mortality was noted after 6 days with 1000 ppm. our principal findings include: loss of body weight (at major doses), central nervous system stimulation, inflammation of lungs and gastro-intestinal tractus, and fatty degeneration of ... | 1978 | 105424 |
| [effect of polychlorinated biphenyls in the rat: ii.--preliminary report on metabolic alterations (author's transl)]. | rats were fed with a diet containing 10 and 100 ppm (wet weight) of phenoclor dpr for 4 weeks in order to evaluate the toxicity of a french pcb mixture. the influence of pcb was studied by determining the liver weights, the hepatic and muscular proteins, glycogen and humidity content as well as hematocrite, glucose, proteins and lipids in the plasma, and the lipidic contents of the liver and the carcass. there was a significant increase in both liver to body weight ratios and liver lipid and pro ... | 1978 | 105425 |
| polychlorinated biphenyls induced rat liver adenomas. | multiple liver adenomas were found in 30 month old female rats which had received for 28 months, 200 ppm polychlorinated biphenyls (arochlor 1254) in their drinking water. no lung metastases were detected. the examination of fragments of these adenomas under the electron microscope showed group polymorphism of the hyperplastic liver cells. the ultrastructural changes were considered to be as a result of chemical lesions induced by polychlorinated biphenyls and as adaptive morphological responses ... | 1978 | 105426 |
| [effect of polychlorinated biphenyls in the rat. iv - metabolic alterations and residual effects of dp6 dietary exposure (author's transl)]. | in the first experiment, rats fed diet containing phenoclor dp6 at a level of 100 ppm (wet weigh) were sacrificed after 1, 3, 8, 15 and 30 days of treatment. metabolic alterations such as enlarged liver, increased protein and lipid liver contents and hyperlipemia were established within 8 days. the glucid metabolism and muscle metabolic parameters show a low sensibility to dp6 treatment. in the second experiment, rats fed diet containing phenoclor dp6 (100 ppm) for 15 days were subsequently remo ... | 1978 | 105429 |
| purine nucleotide metabolism in promastigotes of leishmania tropica: inhibitory effect on allopurinol and analogues of purine nucleosides. | the catabolism of adenosine 5'-monophosphate in promastigotes of l. tropica suggests the presence of a parasite specific pathway. in continuing the investigation on enzymes of this pathway two purine nucleoside cleaving enzymes have been found, adenosine phosphorylase and inosine nucleosidase. various purine nucleoside analogues inhibited the activity of both enzymes. a mode of action of the growth inhibition of l. tropica promastigotes by allopurinol has been suggested on the basis of michaelis ... | 1978 | 105434 |
| [isolation of treponemas from the colon of pigs with clinical dysentery]. | optimal culture conditions in artificial nutritive media were determined for a defined avirulent strain of treponema hyodysenteriae and for four field strains of treponemas in pigs with clinical dysentery. the treponemas were isolated with the use of milliporous filters with pores of 0.3 micrometer in diameter, which were located on the surface of blood agar. no significant difference in the influence of equine, bovine or sheep blood on the growth of treponemas was determined. the commercial amo ... | 1979 | 105448 |
| [comparison of the suitability of phase-contrast and immunofluorescence technics in the laboratory diagnosis of porcine dysentery]. | immunofluorescence technique, compared with the method of phase contrast, does not appear to be better for laboratory diagnostics of swine dysentery because neither of these methods can be used for distinguishing between pathogenic and nonpathogenic strains of treponemas. the number of treponemas contained in faeces should still be considered to be the main criterion in laboratory diagnostics. in clinically healthy pigs from stocks which never suffered from dysentery treponemas were found only i ... | 1979 | 105449 |
| [effect of feeding pelleted feed on the level of volatile fatty acids in the digestive tract of sheep]. | in an experiment with wethers we investigated the effect of complete pelleted feed ration on the concentration of volatile fatty acids in the rumen and intestinal tract. the animals consumed daily 1,300 g of dry matter of a diet which contained 41.81% of meadow hay, 25.28% of barley, 15.37% of sawdust, 14.98% of molasses, 1.32% of urea and 1.24% of a vitamin-mineral supplement for a period of six months. the pelleted diet increased the total amount of volatile fatty acids, the content of propion ... | 1979 | 105451 |
| diseases of chelonians: (1) necropsy survey of tortoises. | the results of necropsies of 144 tortoises of 17 species that died in captivity are presented. intestinal and nutritional disorders (27.0 and 22.2 per cent respectively) were the most common causes of death. nematode infestations were found in 43.8 per cent, but with exception of a few species the parasites were seldom pathogenic. infestations with other helminths appear to be very unusual. protozoan infections were relatively common (at least 22.9 per cent) but protozoa are probably seldom path ... | 1978 | 105454 |
| [precipitins and neutralizing antibodies in marek's disease]. | | 1978 | 105458 |
| [reproductive problems in cattle raising in relation to an increase in its productivity and efficiency]. | | 1978 | 105461 |
| [b. cereus count in meat and dairy food products]. | studies were carried out to establish the contamination of some meat and dairy food products with b. cereus. a total of 48 heat-treated sausages (32 perishable and 16 durable) and 64 batches of pasteurized milk were sampled. it was found that 25 per cent of investigated sausage samples contained b. cereus. perishables proved to a considerable extent more frequently contaminated (33.3 per cent). the count of b. cereus in such products ranged from 10(1) to 10(3) per g. however, pasteurized milk wa ... | 1978 | 105462 |
| shedding of peripheral cytoplasm - a mechanism of liver cell atrophy in human amyloidosis. | a liver biopsy specimen from a case of primary amyloidosis was investigated by electron microscopy. the cytoplasmic periphery of the hepatocytes showed degenerativechanges which are interpreted as indicating shedding of peripheral parts of the cytoplasm. two main variants of this process could be discerned: 1) protrusion and sequestration of hernia-like blebs of cytoplasm, and 2) shedding of vesicles derived from degenerated endoplasmic reticulum. in the latter case transient defects of the plas ... | 1979 | 105463 |
| the effect of dl-2-bromopalmitate on the utilization of palmitic acid by rat granular pneumocytes. | the effect of dl-2-bromopalmitate (brpa), an analogue of palmitic acid (pa), on the utilization of this fatty acid by rat lungs was investigated by a combination of anatomic and biochemical methods. the experiments were performed in vitro on two types of preparations, isolated perfused lungs and lung slices. in the isolated lung preparation the substrate reached the lung via the capillaries, in lung slices via the alveolar epithelium. electron microscope autoradiography showed that brpa depresse ... | 1979 | 105465 |
| studies on ascorbic acid related to the genetic basis of scurvy. | | 1978 | 105472 |
| [absorption of a proteolytic enzyme originating from plants out of the gastro-intestinal tract into blood and lymph of rats (author's transl)]. | the absorption of the proteolytic enzyme bromelain was investigated in adult rats. bromelain was labelled radioactively with 125-iodine and intraduodenally applied. during the observation time of 6 hours blood- and lymphsamples were collected and their radioactivity measured. by radiochromatography high molecular protein could be separated from low molecular protein fragments. by means of a rabbit-anti-bromelainserum bromelain could be identified in the agar-double diffusion technique. results s ... | 1979 | 105480 |
| nitrates and ectopic ventricular activity in mitral valve prolapse: clinical and experimental data. | three patients with mitral valve prolapse, high grade ventricular arrhythmias and a strong family history of sudden death were studied utilizing continuous ecg monitoring and intracardiac stimulation and recording techniques. analysis of 6-hour ambulatory ecgs revealed frequent premature ventricular beats (pvbs) including repetitive and multiform pvbs in each patient. the electrophysiological studies demonstrated normal parameters of intracardiac conduction and refractoriness providing no eviden ... | 1979 | 105483 |
| [the liver as an implantation site for islands of langerhans in experimental diabetes mellitus. morphological and metabolic studies]. | | 1978 | 105489 |
| [changes in the spectrum of disease-causing bacteria]. | | 1978 | 105494 |
| [determination of cholera vibrion enterotoxin by an aggregate hemagglutination method]. | | 1979 | 105503 |
| [serotype characteristics of clinical strains of pseudomonas aeruginosa]. | a total of 348 p. aeroginosa strains isolated from patients with pulmonary and pleural diseases were studied, and 87% of the test showed the possibility of their serotyping with the use of group-specific agglutinating antisera. serogroups ii, iii, iv were found to be prevalent among the strains isolated from patients with bronchopulmonary pathological states. correlations between definite groups of p. aeroginosa in their sensitivity to antibiotics were established; thus, the cultures belonging t ... | 1979 | 105521 |
| [lysozyme concentration in the loose connective tissue of animals with different species sensitivity to brucellosis]. | lysozyme content was determined in the subcutaneous cellular tissue of guinea pigs and albino rats differing by species resistance to brucella infection. experiments were conducted on intact animals and those inoculated subcutaneously with live br. abortus 19-ba vaccine. connective tissue was taken from the site of the vaccine administration and from the contralateral side (control). observations showed connective tissue of the animals highly sensitive to brucella infection to be exceedingly poo ... | 1978 | 105522 |
| [comparative study of allergenic fractions extracted from different strains of staphylococci]. | various methods for obtaining staphylococcus allergens were approbated. biological activity of the preparation proved to depend on the method of obtaining it determining the chemical compostition of the preparation, and on the properties of the strain used. a method of alkaline extraction and a strain with marked pathogenicity signs (strain 209) can be recommended for the preparation of staphylococcus allergen. biological activity of the allergen in case of its approbation in the same dose by pr ... | 1978 | 105524 |
| suppression of uterine motor activity by prostaglandin synthetase inhibitor naproxen. | rats were ovariectomized on day 16 of pregnancy and they were fitted with a pressure sensor uterine microballoon assembly and were given a daily dose of naproxen-sodium. daily uterine activity recordings were taken and subjected to statistical analysis and the rats were carefully checked for either aborting or delivering their fetuses. our data showed that naproxen treatment effectively and significantly prevented the autocatalytic evolution of the uterine motor activity and the regulatory "see- ... | 1978 | 105529 |
| dual action of adrenergic system on the regulation of thyrotrophin secretion in the male rat. | the effect of graded doses of drugs modifying adrenergic activity on basal and cold-stimulated tsh secretion was studied in male rats. alpha-methyl-p-tyrosine (ampt) (16 h before 30 min cold-exposure), phenoxybenzamine (1 h), ca-fusarate (1 h) and diethyldithiocarbamate (ddc) (1 and 18 h) dose-dependently depressed the cold-stimulated tsh secretion. the effect of reserpine (24 h) was not significant. clonidine (1 h), dihydroxyphenyl-serine (dops) (1 h), noradrenaline (na) (1 h), and l-dopa (1 h) ... | 1979 | 105532 |
| catalases and peroxidases histochemical detection; techniques suitable to discriminate these enzymes. | by using the benzidine reaction, on filter paper strips loaded with catalases, peroxidases, porphyrins, haemic iron compounds and iron salts, it was possible to establish 2 histochemical techniques able to detect and discriminate catalases and peroxidases. spot test analytical studies show that only peroxidases oxidize benzidine in presence of a 0.0015 m h2o2 final concentration into the incubation medium. if a 0.0035 m h2o2 final concentration is used both peroxidases and haemic iron were able ... | 1978 | 105550 |
| topochemistry of trace metals in nasal mucosa. potentialities of some histochemical methods and energy dispersive x-ray microanalysis. | histochemical methods and energy dispersive x-ray micro-analysis (edx-analysis) were evaluated in model experiments and on tissue sections for their usefulness in detecting traces of metals in biological tissue. the goal for this study was to establish a method for localization of nickel deposits in the nasal mucosa, where it has been found in concentrations between 1 and 40 microgram/g in nickel exposed individuals. the histochemical methods tested were staining with dimethylglyoxime, rubeanic ... | 1978 | 105552 |
| [the immunhistochemical investigation of trypsin and trypsin-like-activity in the small intestine, pancreas and isolated langerhans islets. a light-, fluorescent- and electron microscopic study (author's transl)]. | by means of the indirect immunofluorescence and immunenzyme technique trypsin and trypsin-like activity in fixed cryostate sections of pancreas and gut and pellets of isolated pancreatic islets respectively were investigated using specific antiserum against trypsin. the enzyme was demonstrated in the cytoplasma of the acinus cells, the epithel cell of the intercalated ducts and the excretory (interlobular) ducts of the exocrine pancreas. by light and fluorescence microscopy we could also localiz ... | 1978 | 105556 |
| testing of the course of neurogenesis and gliogenesis in the germinative zones of the cns of embryonal and early postnatal rats by means of the gel reaction for the histochemical demonstration of the thiamine-pyrophosphatase. histochemical and autoradiographical study. | using the histochemical reaction for the demonstration of tppase the shape and distribution of the golgi apparatus (ga) of the ventricular zones of the cns of rats was studied during embryonal and postnatal development. in the cells of the ventricular zone of the spinal cord, the hypothalamus, the thalamus, the n. caudatus and the cerebral cortex two forms of ga can be distinguished: form 1 ga has the shape of small rods or grains placed in the ventricular process of the cell at various distance ... | 1978 | 105560 |
| angiographic definition of critical coronary artery stenosis. | in summary, it is hazardous to rely solely on coronary angiography to define critical coronary stenosis. clinically, the physiologic significance of a narrowing observed at coronary angiography can best be evaluated by obtaining additional evidence of myocardial ischemia, i.e. stress testing with ecg monitoring, resting and exercise isotope perfusion studies or ventriculographic studies. however, when evaluating coronary angiograms for critical coronary stenosis, several points are worth conside ... | 1979 | 105566 |
| amphipathic antigens of oral microorganisms - immunogenicity and other biological properties. | | 1978 | 105572 |
| adjuvant arthritis in young copper-deficient rats. | adjuvant arthritis has been studied in young copper-deficient rats and a very strong inhibition of the disease following 60 days of deficient diet (0.4 ppm of copper) was found. no difference in lung prostaglandin synthesis and in rat stomach strip and colon reactivity to exogenous prostaglandins was noticed between controls and copper-deficient animals. these results are briefly discussed. | 1978 | 105576 |
| progressive effects of protein-calorie malnutrition on serum free amino acids in infant and young adult nonhuman primates. | serum amino acid profiles have been known to change when dietary protein restriction is instituted in animals and in man. in the present study, significant alterations were noted in infant monkeys observed over 14 weeks. impressive but less dramatic changes were apparent in protein-restricted young adult monkeys observed over a much longer period. in general, essential amino acids were depressed (especially the branched-chain group) while nonessential amino acids tended to rise (especially glyci ... | 1979 | 105624 |
| in-vitro methods for determining minimal lethal concentrations of antimicrobial agents. | to determine the minimal lethal concentration of an antimicrobial agent, broth dilution tests may be performed and then a sample from each tube showing no visible growth may be subcultured onto a drug-free agar medium. by counting the number of viable cells recovered from each tube, the minimal concentration of drug required to kill 99.9% of the cells in the initial inoculum can be determined. studies were undertaken to determine the circumstances under which false-negative tests could occur as ... | 1979 | 105628 |
| case report: intestinal clearance of calcium and protein in waldenstrom's macroglobulinemia. | a 63-year-old man with waldenstrom's macroglobulinemia had severe steatorrhea, marked protein-losing enteropathy, and excessive endogenous fecal calcium clearance. the malabsorption and protein loss resulted in weight loss and hypoalbuminemia. in contrast, the striking enteric calcium loss was completely compensated by an increase in calcium absorption resulting in a positive calcium balance. | 1978 | 105640 |
| freeze-fracture replica of the primate lens fibers. | using freeze-fracture techniques, we studied the fine structure of monkey lens fibers, with special reference to intercellular junctions. gap junctions were frequently observed at the 'ball-and-socket' interdigitations, the 'tongue-and-groove' interdigitations, and between cortical lens fibers, while tight junctions were never apparent. | 1978 | 105647 |
| the number and distribution of mast cells in monkey lungs. | we estimated the number of mast cells in monkey lungs by both quantitative histologic examination and measurement of total lung histamine, and showed that monkey lungs contain between 10(7) and 10(8) mast cells, with approximately 83% of these being located in conducting airways, and 17% in the parenchyma. the number of mast cells found in each airway generation increased from approximately 60,000 in the trachea to 8 million in the terminal bronchioles. in airways from different generations the ... | 1979 | 105654 |
| occurrence of immunoglobulin g-alkaline phosphatase complexes in human serum. | we studied three patients in whom all or part of their serum alkaline phosphatase circulated as a complex with immunoglobulin g (igg). serum alkaline phosphatase isozymes were visualized by their electrophoretic (in agar, agarose, and starch gel and on cellulose acetate), gel filtration, and electroimmunodiffusion behaviour. the alkaline phosphatase-igg complex was of the liver type (two cases) and bone type (one case). the reaction pattern of alkaline phosphatase with different human tissues an ... | 1979 | 105655 |
| peri-oral reflexes in the conscious monkey (macaca mulatta). | | 1978 | 105692 |
| the mechanism of disc pallor in experimental optic atrophy. a fluorescein angiographic study. | ascending optic atrophy was produced in 13 eyes of owl monkeys (aotestrivirgatus) by retinal photocoagulation. color fundus photography and fluorescein angiography were used to study and document the evolution of nerve head abnormalities. the optic nerve heads were also studied histopathologically. except in certain instances of early transient (relative) filling defects, normal disc fluorescent patterns were preserved, despite clinically apparent optic nerve head pallor. sectorial defects did n ... | 1979 | 105695 |
| experimental ciliochoroidal detachment. effect on intraocular pressure and aqueous humor flow. | ciliochoroidal detachments were produced in rhesus monkeys by injecting glutathione bicarbonate ringer's solution, autologous serum, or silicone oil into the anterior suprachoroidal space. silicone oil detachments, like sham operations, caused a transient 2 mm hg reduction in intraocular pressure. detachments with glutathione bicarbonate ringer's solution or serum caused a 6 mm hg reduction in intraocular pressure, which normalized in three weeks. aqueous humor flow in serum-detached eyes was no ... | 1979 | 105696 |
| the inhibition of clostridium chauvoei by bacillus cereus metabolites. | | 1978 | 105701 |
| relationship between genetic variation in thermal stability and electrophoretic mobility of mouse beta-galactosidase. | we have examined the relationships between the genetic determinants for mouse beta-galactosidase heat stability and electrophoretic mobility, in order to clarify previous reports indicating that a variation for enzyme heat stability is restricted to kidney while that for electrophoretic mobility is expressed in all tissues. we find that the two phenotypes show concordant strain distributions and cosegregate in genetic crosses. in contrast to a previous report, the thermal stability variation is ... | 1978 | 105715 |
| an anthranilate synthase of the extreme aminase type in a species of blue-green bacteria (algae). | anthranilate synthase of agmenellum quadruplicatum, a unicellular species of blue-green bacteria, consists of two nonidentical subunits. a 72,000 dalton protein has aminase activity but is incapable of reaction with glutamine (amidotransferase) unless a second protein (18,000 molecular weight) is present. the small subunit was first detected through its ability to complement a partially purified aminase subunit from bacillus subtilis to produce a hybrid complex capable of amidotransferase functi ... | 1978 | 105718 |
| the necessity of magnesium cation for acid assistance aglycone departure in catalysis by escherichia coli (lacz) beta-galactosidase. | 1. removal of mg2+ from escherichia coli (lacz) beta-galactosidase slightly increases the rate of hydrolysis of galactosyl pyridinium salts, but decreases the rate of hydrolysis of arylgalactosides. 2. fair correlation of logkcat. and log (km) with the pka of aglycone is now observed for arglygalactosides, as well as for glycosyl pyridinium salts. 3. degalactosylation of mg2+-free enzyme is the rate-limiting step in the hydrolysis of 2,4-dinitrophenyl galactoside. 4. alpha-deuterium kinetic isot ... | 1978 | 105722 |
| substrate specificity of 5'-methylthioadenosine phosphorylase from human prostate. | 5'-methylthioadenosine phosphorylase was purified approx. 340-fold from human prostate by using affinity chromatography by hg-coupled sepharose. the enzyme, responsible for the breakdown of 5'-methylthioadenosine into adenine and methylthioribose 1-phosphate, was partially characterized. the apparent km for 5'-methylthioadenosine is 25 microm. it is activated by thiols and shows an absolute requirement for phosphate ions. new analogues of 5'-methylthioadenosine were prepared and their activity a ... | 1978 | 105725 |
| the exocellular dd-carboxypeptidase-endopeptidase of streptomyces albus g. interaction with beta-lactam antibiotics. | kinetically, the three-step model proposed for the interaction between beta-lactam antibiotics and the exocellular dd-carboxypeptidases-transpeptidases of streptomyces r61 and actinomadura r39 [frère, ghuysen & iwatsubo (1975) eur. j. biochem. 57, 343--357; fuad, frère, ghuysen, duez & iwatsubo (1976) biochem. j. 155, 623--629] applies to the interaction between the much less penicillin-sensitive exocellular dd-carboxypeptidase-endopeptidase of streptomyces albus g and at least phenoxymethylpeni ... | 1978 | 105727 |
| n-acetyl-beta-d-glucosaminidase in marmoset kidney, serum and urine. | n-acetyl-beta-d-glucosaminidase activities were determined in homogenates of marmoset kidney, in serum and in urine by using the 4-methylumbelliferyl substrate. the enzyme activity was separated into several components by deae-cellulose ion-exchange chromatography, starch-gel electrophoresis and isoelectric focusing. the kidney contained two major forms of the enzyme, a and b, which had similar ph optima and km values. the a-form bound to deae-cellulose at ph 6.8, migrated towards the anode on s ... | 1978 | 105728 |
| the acidic ribosomal phosphoprotein of eukaryotes and its relationship to ribosomal proteins l7 and l12 of escherichia coli. | the acidic ribosomal phosphoprotein, lgamma, of krebs ii ascites cells was further characterized and compared with proteins l7 and l12 of escherichia coli. ribosomal protein lgamma was selectively removed from 60s sibosomal subunits by 50% ethanol and 1m-nh4cl, and antibodies raised against protein lgamma cross-reacted with e. coli protein l12 in immunodiffusion experiments. these and other, previously reported, data support the proposal that the uekaryotic counterpart of e. coli proteins l7 and ... | 1978 | 105730 |
| extracellular labeling of growing secreted polypeptide chains in bacillus subtilis with diazoiodosulfanilic acid. | studies of the mechanism of protein secretion in a gram-positive bacterium, bacillus subtilis, yielded results very similar to those previously obtained with a gram-negative organism: nascent chains protruding from protoplasts could be labeled extracellularly; the labeled chains could be recovered on polysomes isolated from the membrane--polysome fraction; they could be released by puromycin, low mg2+, or chain completion; the completed chains include a known secreted protein (alpha-amylase); an ... | 1979 | 105750 |
| unique conformational properties of muscle carbonic anhydrase iii as demonstrated by circular dichroism spectrometry. | | 1979 | 105751 |
| comparative in vivo nitrogen-15 nuclear magnetic resonance study of the cell wall components of five gram-positive bacteria. | the proton-decoupled 9.12 mhz 15n nmr spectra of 15n-labeled bacillus subtilis, bacillus licheniformis, staphylococcus auresu, streptococcus faecalis, and micrococcus lysodeikticus intact cells, isolated cells walls, and cell wall digests have been examined. the general characteristics of gram-positive bacteria 15n nmr spectra and described and spectral assignments are provided, which allow in vivo 15n nmr to be applied to a wide range of problems in bacterial cell wall research. the qualitative ... | 1979 | 105756 |
| membrane changes during growth of tetrahymena in the presence of ethanol. | after a brief lag period for acclimation, tetrahymena pyriformis, strain nt-1, is capable of growing in culture medium containing high levels of ethanol. when grown in a medium having 1.6% ethanol, the membrane phospholipid composition was significantly different from that of control cells. the principal changes included a reduction in hexadecenoic acids (16 : 1 and 16 : 2) from 23% to 5% and an increase in linoleic acid (18 : 2) from 14% to 25% similar but less pronounced changes were observed ... | 1979 | 105757 |
| further observations on the initiation of sperm motility. | | 1978 | 105770 |
| radioimmunoassay for luteinizing hormone (lh) in the ring-tailed lemur (lemur catta) with antiovine lh and ovine 125i-lh. | | 1978 | 105771 |
| multiple sclerosis: immunochemical studies on the demyelinating serum factor. | sera from patients with multiple sclerosis (ms) frequently produce demyelination of central nervous system tissue cultures. the nature of the factors responsible for demyelination is not as yet clearly established. however, several authors previously reported, in in vivo and in vitro models, demyelinating activity in igg fractions isolated from sera and cerebrospinal fluid of ms patients14,42,48. we found the demyelinating activity of ms sera to be extremely labile to conventional biochemical tr ... | 1979 | 105780 |
| distribution of trigeminothalamic projection cells in cat and monkey. | | 1979 | 105782 |
| thermostable antigens from the bone marrow of several animal species. | studies on bone marrow extracts from ten animal species have revealed the presence of thermostable antigens. similar antigens were found in other organs as shown by immunodiffusion and cross-reactivity was demonstrated between the thermostable proteins from horse and dog and between cow and sheep proteins. using an immunoenzymatic reaction with glucose oxidase as the marker, the thermostable antigens were localized in the blood and bone marrow polymorphonuclear leukocytes and the myeloid cell li ... | 1978 | 105786 |
| the effect of iodine deficiency and propylthiouracil on the hypothalamo--pituitary--thyroid axis in the neonatal rat. | | 1978 | 105791 |
| production and characterization of two hemolysins of bacillus cereus. | bacillus cereus strain b-48 produced two hemolysins with molecular weights of 52,000 (h-i) and 31,000 (h-ii). a mutant was isolated that produced only h-ii but was identical with the wild type in all other respects. we exploited this mutant to produce h-ii for study that was free of contamination by h-i. by manipulation of media composition, we produced h-i in the absence of h-ii. the hemolysins were precipitated differently by ammonium sulfate, and both exhibited the arrhenius effect when heate ... | 1978 | 105792 |
| comparison of metabolism-mediated binding to dna of 7-hydroxymethyl-12-methylbenz(a)anthracene and 7, 12-dimethylbenz(a)anthracene. | comparison of the binding to dna of 7-hydroxymethyl-12-methylbenza(a)anthracene and 7, 12-dimethylbenz(a)anthracene (dmba) catalyzed by mouse embryo cells in culture or by rat liver microsomes indicates that the products formed are different for the two hydrocarbons. thus, the hydroxy compound is not an intermediate in the binding of dmba to dna in these systems. binding of the hydroxy compound to dna in mouse embryo cells is less efficient than for dmba and is inhibited by 1,1,1-trichloropropy ... | 1979 | 105803 |
| animal models for the comparative assessment of neurotoxicity following repeated administration of vinca alkaloids. | neurotoxicity is the major side effect occurring during the clinical use of vincristine (vcr). animal models predictive of potential neurotoxicity would be very useful in the preclinical development of new vinca compounds. to simulate conditions in which neurotoxicity is produced during the clinical use of vcr, experimental animals (except the guinea pig) were given the test compounds by the iv route over a prolonged time period. doses were selected based on the production of leukopenia. vindesi ... | 1979 | 105807 |
| epithelial differentiation and taste buds in the soft palate of the monkey, macaca irus. | a combination of light, transmission and scanning electron microscopy was employed to demonstrate the occurrence, arrangement and structure of taste buds in the oral mucosa of the soft palate of monkeys (macaca irus). taste buds are found in aggregates confined to 0.15 to 0.3 mm wide, round islands of keratinizing epithelium embedded in the normally non-keratinizing integument. topography, configuration and structure of these epithelial islands and their taste buds are described, and the questio ... | 1979 | 105810 |
| [purfication and molecular weight of an rna-dependant rna polymerase from brassicae oleracea var. botrytis]. | an rna-dependent rna polymerase has been completely purified from cauliflower inflorescences. analysis of the purified enzyme on sds-polyacrylamide gels showed one polypeptide chain with a molecular weight of 140,000 dalton. the enzyme is monomeric in its native state. the in vitro activity was completely dependent on added rna, the most efficient templates being poly (u), poly (u, c), poly (i) and viral rna. | 1978 | 105817 |
| [serum iodothyronine concentrations and properties of mitochondria phosphorylative oxidations in old rats]. | serum concentrations of t4 and t3 in 2 year-old rats were decreased by about 30% compared to 2 month-old animals, but rt3 was similar. activities of old rats' liver mitochondria were near those of young thyroidectomized animals. low circulating t3 partly explains metabolic changes observed during senescence. | 1978 | 105818 |
| [structure-activity relationship of various ephedrine isomers on noradrenaline uptake by the isolated perfused rabbit heart]. | (-)ephedrine, (+) ephedrine, (-) psi ephedrine and (+) psi ephedrine inhibit both the neuronal and extraneuronal uptakes of noradrenaline in isolated rabbit heart. (-) ephedrine is the most active isomer for the inhibition of both neuronal and extraneuronal uptakes. the relative inhibition potential of the three other isomers is not the same when applied to one or other uptake. | 1978 | 105824 |
| a controlled clinical trial comparing disodium cromoglycate and ici 74,917 in extrinsic asthma. | a controlled clinical trial comparing inhalation of disodium cromoglycate (dscg) and of ici 74,917 was carried out using a double-placebo technique in thirty-two patients with extrinsic asthma already shown to be reasonably responsive to dscg. in view of the fact that ici 74,917 is 300 times more potent than dscg in inhibiting antigenic challenge in animals a better effect was anticipated from the new drug in the human asthmatic subject. whilst this was not obtained, there appeared to be no less ... | 1979 | 105825 |
| an epidemiologic observation post for early detection of influenza epidemics. | | 1979 | 105846 |