| genetic diversity of ehrlichia canis strains from naturally infected dogs in rio de janeiro, brazil. | the aim of this study was to characterize ehrlichia canis strains from naturally infected dogs in rio de janeiro, brazil. in addition, all the clinical and hematological findings observed in these dogs were reported. pcr targeting the 16s rrna gene was used for diagnostic purposes, and the trp19 and trp36 genes were sequenced to evaluate the genetic diversity. fifteen samples were positive for e. canis. the polymerase chain reaction for the trp19 gene resulted in 11 amplicons (11/15), which were ... | 2016 | 25271448 |
| development of antibodies to and pcr detection of ehrlichia spp. in dogs following natural tick exposure. | dogs exposed to ticks in the southern us may become infected with multiple species of ehrlichia. to better define infection risk, blood samples collected from 10 dogs infested with ticks via a natural infestation model were evaluated by blood smear examination, pcr, patient-side elisas (snap® 4dx® and snap® 4dx® plus), ifa, and peptide based elisa for evidence of infection with ehrlichia canis, e. chaffeensis, and/or e. ewingii. although morulae were rarely identified in blood smears, every dog ... | 2014 | 25213230 |
| colorimetric detection of ehrlichia canis via nucleic acid hybridization in gold nano-colloids. | canine monocytic ehrlichiosis (cme) is a major thick-bone disease of dog caused by ehrlichia canis. detection of this causal agent outside the laboratory using conventional methods is not effective enough. thus an assay for e. canis detection based on the p30 outer membrane protein gene was developed. it was based on the p30 gene amplification using loop-mediated isothermal dna amplification (lamp). the primer set specific to six areas within the target gene were designed and tested for their se ... | 2014 | 25111239 |
| cold agglutinin activity in 2 dogs. | a 5-year-old neutered male mastiff and an 8-year-old spayed female labrador retriever were presented to the university of minnesota veterinary medical center. the mastiff was presented for evaluation of lameness and pyoderma one month prior in missouri, where he tested positive for ehrlichia canis by serum elisa test, treated with doxycycline. pcr for ehrlichia sp, anaplasma sp, babesia sp, and bartonella sp, and pcr for antigen receptor rearrangement were negative, serum protein electrophoresis ... | 2014 | 25056251 |
| use of an automated system for detection of canine serum antibodies against ehrlichia canis glycoprotein 36. | ehrlichia canis is the most common cause of monocytotropic ehrlichiosis in dogs around the world. the purpose of the present study was to validate a new automated fluorescence system (accuplex4™ biocd system; antech diagnostics, lake success, new york) to detect antibodies against the e. canis immunodominant glycoprotein 36 (gp36). sera and blood samples (ethylenediamine tetra-acetic acid) were collected from mixed sex beagles ( n = 8) on days 0, 3, 7, 10, 14, 17, 21, 28, 42, 49, 56, 63, 70, 77, ... | 2014 | 25027497 |
| clinical history and hematological findings among canines with monocytic ehrlichiosis. | canine monocytic ehrlichiosis is a tick borne disease caused by ehrlichia canis, an obligate intracellular rickettsial organism belonging to the family anaplasmataceae. canine ehrlichiosis causes hemaotological changes among infected animals which could be used as a potential predictor for diagnosing canine monocytic ehrlichiosis (cme). ninety-four blood samples were obtained from canines that either presented for a routine health check-up or for clinical illness. a history, physical and laborat ... | 2014 | 24964665 |
| the diagnosis and management of a case of leishmaniosis in a dog imported to australia. | this case study discusses in detail for the first time the diagnosis and management of a case of leishmaniosis in a dog imported to australia. the dog presented with epistaxis and a non-regenerative anaemia five years after being imported from europe. protozoa were identified within macrophages in bone marrow and splenic cytology. a leishmania indirect fluorescent antibody test was performed and was positive while an ehrlichia canis antibody test was negative. polymerase chain reaction of the it ... | 2014 | 24768315 |
| molecular and serological detection of ehrlichia canis in naturally exposed dogs in iran: an analysis on associated risk factors. | the general aim of this study, which was conducted for the first time in iran, was to evaluate the seroprevalence and geographical distribution of ehrlichia canis in a dog population in iran, followed by molecular confirmation using pcr and sequencing. blood samples were collected from 240 dogs in different areas of alborz and tehran provinces and initially analyzed using the immunofluorescent antibody (ifa) test to detect anti-ehrlichia canis igg antibodies. subsequently, nested pcr was perform ... | 2014 | 24728356 |
| in vitro culture and structural differences in the major immunoreactive protein gp36 of geographically distant ehrlichia canis isolates. | ehrlichia canis, the etiologic agent of canine ehrlichiosis, is an obligate intracytoplasmic gram-negative tick-borne bacterium belonging to the anaplasmataceae family. e. canis is distributed worldwide and can cause serious and fatal infections in dogs. among strains of e. canis, the 16s rrna gene dna sequences are highly conserved. using this gene to genetically differentiate isolates is therefore difficult. as an alternative, the gene gp36, which encodes for a major immunoreactive protein in ... | 2014 | 24713279 |
| in vitro culture of a novel genotype of ehrlichia sp. from brazil. | ehrlichiae are obligate intracytoplasmic gram-negative, tick-borne bacteria belonging to the anaplasmataceae family. ehrlichioses are considered emerging diseases in both humans and animals. several members of the genus ehrlichia have been isolated and propagated in vitro. this study describes the continuous propagation of a brazilian ehrlichia sp. isolate in ide8 tick cells, canine dh82 cells and bovine aorta cells. initially, the organisms were isolated from the haemolymph of a rhipicephalus ( ... | 2013 | 24589106 |
| serum canine pancreatic lipase immunoreactivity in experimentally induced and naturally occurring canine monocytic ehrlichiosis (ehrlichia canis). | ehrlichia canis infection causes multisystemic disease in dogs (canine monocytic ehrlichiosis, cme) which is associated with variable morbidity and mortality. atypical clinical manifestations, including gastrointestinal signs, may occasionally occur in cme and approximately 10-15% of dogs are presented with historical or clinical evidence of vomiting, diarrhea, and/or abdominal discomfort. the objective of this study was to investigate if there are any alterations in serum canine pancreatic lipa ... | 2014 | 24530039 |
| hematological values associated to the serological and molecular diagnostic in cats suspected of ehrlichia canis infection. | the literature contains several studies on feline ehrlichiosis. however, information about the characteristics of ehrlichia infection in cats is still scanty. this study evaluated the association between ehrlichia spp. infection and the hematologic data of 93 cats treated at the federal university of mato grosso veterinary hospital in cuiabá, state of mato grosso, brazil. the presence of or exposure to ehrlichia spp. infection was evaluated by polymerase chain reaction (pcr) targeting the dsb an ... | 2014 | 24473870 |
| the spreading process of ehrlichia canis in macrophages is dependent on actin cytoskeleton, calcium and iron influx and lysosomal evasion. | ehrlichia canis is an obligate intracellular microorganism and the etiologic agent of canine monocytic ehrlichiosis. the invasion process has already been described for some bacteria in this genus, such as e. muris and e. chaffeensis, and consists of four stages: adhesion, internalisation, intracellular proliferation and intercellular spreading. however, little is known about the spreading process of e. canis. the aim of this study was to analyse the role of the actin cytoskeleton, calcium, iron ... | 2014 | 24378068 |
| subtractive phage display selection from canine visceral leishmaniasis identifies novel epitopes that mimic leishmania infantum antigens with potential serodiagnosis applications. | visceral leishmaniasis (vl) is a zoonotic disease that is endemic to brazil, where dogs are the main domestic parasite reservoirs, and the percentages of infected dogs living in regions where canine vl (cvl) is endemic have ranged from 10% to 62%. despite technological advances, some problems have been reported with cvl serodiagnosis. the present study describes a sequential subtractive selection through phage display technology from polyclonal antibodies of negative and positive sera that resul ... | 2014 | 24256622 |
| usefulness of a rapid immuno-migration test for the detection of canine monocytic ehrlichiosis in africa. | a rapid immuno-migration test for the serological detection of canine monocytic ehrlichiosis, witness® ehrlichia (we) (zoetis, france), was evaluated in 528 serum samples from dogs living in endemic areas of west and east africa: senegal (n=208), ivory coast (n=7), sudan (n=27), and djibouti (n=286). of these dogs, 200 were french military working dogs (mwd) temporarily residing in africa. the we test results were compared with those obtained by indirect immunofluorescence (ifa). the sensitivity ... | 2014 | 24252768 |
| inhibitory effect of interferon gamma on frequency of ehrlichia canis-infected cells in vitro. | ehrlichia canis is an obligate intracellular bacterium that infects the macrophage-monocyte cells of dogs, causing canine monocytic ehrlichiosis. interferon-γ (ifn-γ), along with other cytokines, mediates the immune response to such intracellular bacterial invasions. to determine the role of ifn-γ in the immunity of dogs to e. canis infection, peripheral blood mononuclear cells (pbmc) and white blood cells (wbc) were collected from e. canis-infected dogs and added to a culture of e. canis in dh8 ... | 2013 | 24148826 |
| ultrastructure of ehrlichia mineirensis, a new member of the ehrlichia genus. | recently, we reported the in vitro isolation and the molecular characterization of a new species of ehrlichia (ehrlichia mineirensis) from haemolymph of brazilian rhipicephalus (boophilus) microplus ticks. this organism shows an ortholog of ehrlichia canis major immunogenic protein gp36 with a new structure of tandem repeats. in the present study, we used electron microscopy (high pressure freezing and freeze substitution preparative techniques) to characterize morphologically this new agent gro ... | 2013 | 23998429 |
| transmission of ehrlichia canis by rhipicephalus sanguineus ticks feeding on dogs and on artificial membranes. | a south african strain of ehrlichia canis was isolated and used to infect a laboratory-bred beagle dog. rhipicephalus sanguineus nymphs, which fed on this dog, moulted to adult ticks which carried infection rates of e. canis between 12% and 19% and were used in a series of in vivo and in vitro experiments. five groups of 6 dogs were challenged with the infected r. sanguineus ticks, which were removed 24h, 12h, 6h or 3h after the ticks had been released onto the dogs. the animals were monitored f ... | 2013 | 23962826 |
| influence of experimental canine ehrlichiosis on the e-ada activity and purine levels in serum and possible functional correlations with pathogenesis. | the aim of this study was to evaluate adenosine deaminase activity and purines levels in serum of dogs experimentally infected by ehrlichia canis. banked serum samples of dogs divided into two groups with five animals each: healthy animals and animals infected by e. canis. the concentration of purines (adenosine triphosphate (atp), adenosine diphosphate (adp), adenosine monophosphate (amp), adenosine, inosine, hypoxanthine, xanthine and uric acid), and adenosine deaminase (e-ada) activity in ser ... | 2013 | 23907060 |
| imidacloprid 10 % / flumethrin 4.5 % collars (seresto®, bayer) successfully prevent long-term transmission of ehrlichia canis by infected rhipicephalus sanguineus ticks to dogs. | the objective of this study was to determine the empirical efficacy of imidacloprid 10 %/flumethrin 4.5 % (seresto®) collars in preventing long-term transmission of ehrlichia canis by infected rhipicephalus sanguineus ticks to dogs. the study was a parallel group design, single centre, randomised, non-blinded, controlled, long-term efficacy study. the treatment group of 8 dogs was fitted with seresto® collars, the untreated control group of 8 dogs received no collars. ehrlichia canis-infected ti ... | 2013 | 23774841 |
| ehrlichia canis infection in a dog with no history of travel outside the united kingdom. | a two-year-old female neutered tibetan terrier was referred following a one-month history of lethargy, inappetence and pancytopenia, which had been poorly responsive to immunosuppressive and fluoroquinolone treatment. the dog was diagnosed with pure red cell aplasia and was found to be positive for ehrlichia canis by both antibody titre measurement and polymerase chain reaction. the dog lived in london and had not travelled outside the uk. the dog was treated with doxycycline, prednisolone and c ... | 2013 | 23718904 |
| increase nitric oxide and oxidative stress in dogs experimentally infected by ehrlichia canis: effect on the pathogenesis of the disease. | the aim of this study was to evaluate nitric oxide levels, lipid peroxidation, protein oxidation and glutathione reductase activity in serum of dogs experimentally infected by ehrlichia canis. banked serum samples of dogs divided into two groups were used: negative control (n=5) and infected by e. canis (n=5). the concentration of nitrite/nitrate (nox), lipid peroxidation (tbars), advanced oxidation protein products (aopp), and glutathione reductase (gr) activity in sera were evaluated. samples ... | 2013 | 23540584 |
| molecular detection of ehrlichia canis in dogs from the pantanal of mato grosso state, brazil. | the present study evaluated the presence of ehrlichia dna in the blood samples of 320 dogs from the urban and rural areas of the municipality of poconé, pantanal region, mato grosso state, by polymerase chain reaction (pcr), targeting the ehrlichial dsb gene. risk factors for infection in dogs were also evaluated. forty-eight (15%, 95% ci: 11.4-19.5%) dogs were positive: 25 (15.6%, 95% ci: 10.4-22.2%) from the urban area and 23 (14.4%, 95% ci: 9.3-20.8%) from the rural area (p > 0.05). partial d ... | 2013 | 23538496 |
| genetic diversity of ehrlichia canis in brazil. | canine monocytic ehrlichiosis is a highly prevalent disease in brazil, where the genetic diversity of ehrlichia canis remains undefined. in this study, we used the trp36 gene to examine the genetic diversity of e. canis strains from naturally infected dogs residing in five distinct geographic regions in brazil. e. canis dna was detected in 82/126 (65%) dogs by dsb-specific pcr and e. canis was isolated in cell culture from 13 dogs. sequences obtained from dsb genes amplified from the isolates we ... | 2013 | 23490559 |
| efficacy of rifampicin in the treatment of experimental acute canine monocytic ehrlichiosis. | to assess the efficacy of rifampicin in achieving clinical and haematological recovery and clearing infection in dogs with experimentally induced acute monocytic ehrlichiosis. | 2013 | 23475646 |
| prevention of transmission of ehrlichia canis by rhipicephalus sanguineus ticks to dogs treated with a combination of fipronil, amitraz and (s)-methoprene (certifect®). | the ability of certifect(®) (a combination of fipronil, amitraz and (s)-methoprene) to prevent transmission of ehrlichia canis was studied in two groups of eight dogs. one group was treated with certifect while the other group remained untreated. all dogs were exposed to e. canis-infected rhipicephalus sanguineus ticks on days 7, 14, 21 and again on day 28 post-treatment by releasing ticks into the kennels of the dogs to simulate the natural way of infestation. animals were examined in situ for ... | 2013 | 23298566 |
| [molecular identification of ehrlichia canis in a dog from arica, chile]. | we report a molecular confirmed case of canine ehrlichiosis caused by ehrlichia canis. a 10-year old female crossbred siberian from the city of arica, which was infested by ticks, presented hemorrhagic manifestations (hematomas and snout bleeding) and prostration. blood cell count revealed thrombocytopenia (30,000 platelets/ mm³). immunochromatographic rapid testing for e. canis igg was positive. amplification and sequencing of a fragment of the 16s rrna gen from a blood sample showed 100% homol ... | 2012 | 23282495 |
| serological survey on ehrlichia sp. among dogs in the central region of rio grande do sul. | a serological survey on ehrlichia canis was conducted among dogs in the central area of the state of rio grande do sul, where the tick rhipicephalus sanguineus is a common parasite of dogs. out of a total of 316 dogs attended at the veterinary teaching hospital in the municipality of santa maria, only 14 (4.43%) reacted positively to e. canis antigens in the indirect immunofluorescence assay, with the following endpoint titers: 80 (three dogs), 160 (five), 320 (four), 640 (one) and 1280 (one). l ... | 2013 | 23184320 |
| evaluation of lymphocyte populations in dogs naturally infected by ehrlichia canis with and without clinical signs. | immune response elicited by the host during ehrlichial infections could influence the clinical signs and laboratory and pathological findings. twenty-eight dogs naturally infected by ehrlichia canis were included in this study. twenty of them presented only laboratory findings traditionally associated with canine monocytic ehrlichiosis (cme), whilst 8 dogs also showed clinical signs classically associated with cme (pale mucous membranes, fever, lymphadenopathy, weight loss, anorexia, lethargy or ... | 2012 | 23168046 |
| ehrlichia canis in dogs in a semiarid region of northeastern brazil: serology, molecular detection and associated factors. | this study investigated infection by ehrlichia spp. agents by pcr, immunofluorescence assay test (ifat), and by giemsa-stained blood smears in 108 dogs from a semiarid region of the state of paraíba, northeastern brazil. seventy-five (69.4%) of the 108 dogs were found to be seropositive to ehrlichia canis, while only four dogs (3.7%) were positive in real-time pcr for e. canis. in six dogs (5.6%) e. canis-like morulae were observed in monocytes. animals that stayed in environment whose floor was ... | 2013 | 23141416 |
| ehrlichia canis in rhipicephalus sanguineus ticks in the ivory coast. | canine monocytic ehrlichiosis caused by ehrlichia canis is distributed globally, but its prevalence in africa is poorly known. in the study reported herein, 27% of rhipicephalus sanguineus ticks collected from watchdogs in abidjan, ivory coast, were positive for e. canis using quantitative real-time pcr. a new molecular strategy is proposed that can be used not only for epidemiological study, but also for the diagnosis of canine monocytic ehrlichiosis. our findings show for the first time the pr ... | 2012 | 23141106 |
| first detection of ehrlichia canis in rhipicephalus bursa ticks in sardinia, italy. | canine monocytic ehrlichiosis is a common and widespread disease. this disease is present in sardinia year-round because the temperate weather on the island permits the survival of many types of tick vectors. a total of 35 ticks was collected from goats in south-eastern sardinia, italy, and tested for ehrlichia dna using polymerase chain reaction and dna sequencing. this study provides the first evidence of the presence of ehrlichia canis in rhipicephalus bursa ticks. the data presented here inc ... | 2012 | 23140896 |
| in silico characterization of three two-component systems of ehrlichia canis and evaluation of a natural plant-derived inhibitor. | two-component signal transduction systems (tcs) are important elements in the interaction of endobacteria with host cells. they are basically composed of two proteins, an environmental signal sensor and a response regulator, which activate genes involved in a wide range of bacterial responses to their environment. we analyzed three sets of genes corresponding to tcs of ehrlichia canis, a common tick-borne canine pathogen and the etiologic agent of canine monocytic ehrlichiosis, in order to ident ... | 2012 | 23096683 |
| evaluation of an attenuated strain of ehrlichia canis as a vaccine for canine monocytic ehrlichiosis. | canine monocytic ehrlichiosis is an important tick-borne disease worldwide. no commercial vaccine for the disease is currently available and tick control is the main preventive measure against the disease. the aim of this study was to evaluate the potential of a multi-passaged attenuated strain of ehrlichia canis to serve as a vaccine for canine monocytic ehrlichiosis, and to assess the use of azithromycin in the treatment of acute ehrlichiosis. twelve beagle dogs were divided into 3 groups of 4 ... | 2012 | 23072894 |
| experimental ehrlichia canis infection changes acute-phase proteins. | early diagnosis of canine ehrlichiosis favors prompt institution of treatment and improves the prognosis for the animal, since this disease causes mortality among dogs. studies have shown that determining the concentration of acute-phase proteins (apps) may contribute towards early detection of disease and aid in predicting the prognosis. this study aimed to evaluate the app profile in dogs experimentally infected with ehrlichia canis, at the start of the infection and after treatment. it also i ... | 2012 | 23070428 |
| first detection and molecular characterization of ehrlichia canis from dogs in nigeria. | the present study aimed to detect the presence of ehrlichia canis in naturally infected dogs in nigeria, using a combination of pcr and sequence analysis of the 16s rrna gene and two genes encoding the tandem repeat-containing proteins (trps), trp19 and trp36. out of a total of 100 blood samples collected from domestic dogs presented to veterinary hospitals in jos, the capital city of plateau state of nigeria, 11 were positive in nested pcr for e. canis. sequencing results for these amplicons sh ... | 2013 | 22925936 |
| mitochondrial dna analysis of rhipicephalus sanguineus sensu lato (acari: ixodidae) in the southern cone of south america. | a genetic analysis of partial sequences of the mitochondrial 16s and 12s rdna genes of rhipicephalus sanguineus sensu lato collected in the southern cone of south america was carried out. also, sequences of ticks belonging to this taxon from europe, africa and other south american localities were included. tcs networks constructed with 16s rdna sequences showed two clusters of haplotypes, namely, southern lineage (st) and northern lineage (nt). haplotypes representing the specimens coming from l ... | 2012 | 22818199 |
| haematological parameters in stray dogs seropositive and seronegative to ehrlichia canis in north trinidad. | in view of the fact that stray dogs are a reservoir for many diseases, this study was undertaken to determine the prevalence of ehrlichia canis in stray dogs in north trinidad and to evaluate the diagnostic implications of haematological alterations associated with seropositivity. overall, 41 (44.6%) of 92 stray dogs were seropositive to e. canis by the indirect immunofluorescent antibody test. dogs, one year of age and older (59.7%) were more likely to be seropositive than dogs less than one ye ... | 2012 | 22658916 |
| pulmonary hypertension associated with ehrlichia canis infection in a dog. | | 2012 | 22645155 |
| serum cardiac troponin i concentrations in naturally occurring myelosuppressive and non-myelosuppressive canine monocytic ehrlichiosis. | the aim of this study was to determine whether serum cardiac troponin i (ctni) concentration measured on admission was associated with the clinical severity of canine monocytic ehrlichiosis and was predictive of clinical outcome (death or survival) in dogs naturally infected with ehrlichia canis. serum ctni concentration was compared among 22 dogs with non-myelosuppressive ehrlichiosis (nme), 22 dogs with myelosuppressive ehrlichiosis (me) and 10 healthy dogs. unlike healthy dogs, 45.5% nme and ... | 2012 | 22633176 |
| direct detection of ehrlichia canis by pcr in the conjunctiva of a dog with bilateral anterior uveitis. | the following report describes the direct detection of ehrlichia canis by real-time pcr in the conjunctiva of a 1-year-old female maltese dog. after being imported from brazil, the dog was presented because of anorexia, dehydration, fever, and palpable mandibular lymph nodes. a few days later, the dog developed bilateral blepharospasm, photophobia and anterior uveitis. monocytic ehrlichia was diagnosed by a positive pcr result and the detection of igm and igg antibodies. because of the massive u ... | 2012 | 22563611 |
| evaluation of peripheral blood lymphocyte subsets in family-owned dogs naturally infected by ehrlichia canis. | previous research suggested that clinical manifestations, histopathological lesions, and even infection maintenance in the course of canine monocytic ehrlichiosis (cme) are directly related to the immune response developed by the host. in the present study, blood lymphocyte subsets were analyzed by multiparametric flow cytometry in 37 dogs with naturally occurring cme and 47 healthy dogs used as controls. t, t helper (th), t cytotoxic (tc), b, non-t, non-b lymphocytes and those that express mhc ... | 2012 | 22521727 |
| the role of cytoskeleton, components of inositol phospholipid signaling pathway and iron in ehrlichia canis in vitro proliferation. | ehrlichia canis, etiologic agent of canine monocytic ehrlichiosis, is an obligatory intracellular bacterium that parasitizes monocytes and macrophages. in this study we analyzed the role of the cytoskeleton specifically actin microfilaments and microtubules, components of inositol phospholipid signaling pathway such as phospholipase c (plc), protein kinase (ptk) and calcium channels as well as the role of iron in the e. canis proliferation in dh82 cells. different inhibitory compounds were used ... | 2012 | 22415481 |
| ehrlichia canis infection in two dogs that emigrated from endemic areas. | two dogs, emigrated from zambia and china to japan, were diagnosed with ehrlichia canis infection. both cases had thrombocytopenia, non-regenerative anemia, and hypergloblinemia with polyclonal gammopathy. case 1 had ataxia of the hind limbs. severe meningitis was revealed by magnetic resonance imaging examination. intracytoplasmic inclusions were observed in mononuclear cells of cerebrospinal fluid. case 2 had a history of bilateral epistaxis, and severe pancytopenia was noticed in complete blo ... | 2012 | 22214859 |
| the prevalence of anaplasma platys and a potential novel anaplasma species exceed that of ehrlichia canis in asymptomatic dogs and rhipicephalus sanguineus in taiwan. | canine anaplasmosis is regarded as an infection by anaplasma platys rather than zoonotic anaplasma phagocytophilum in subtropical areas based on the assumption that the common dog tick species is rhipicephalus sanguineus, which transmits e. canis and presumably a. platys. we investigated asymptomatic dogs and dog ticks from 16 communities in nantou county, taiwan to identify common dog tick species and to determine the prevalence of anaplasma and ehrlichia spp. of total 175 canine blood samples ... | 2017 | 28781347 |
| molecular investigation and phylogeny of anaplasmataceae species infecting domestic animals and ticks in corsica, france. | corsica is a french island situated in the mediterranean sea. the island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. the family anaplasmataceae is a member of the order rickettsiales; it includes the genera anaplasma, ehrlichia, neorickettsia and wolbachia. anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera anaplasma and ehrlichia. the a ... | 2017 | 28645313 |
| a new pcr assay for the detection and differentiation of babesia canis and babesia vogeli. | babesia spp. are globally distributed tick-borne protozoan parasites that infect the red blood cells of a wide range of vertebrate hosts, including humans. diagnosis of babesiosis is often impeded by the transient presence of the parasites in peripheral blood, as well as by their pleomorphic nature. given the reports of an expanding and, in some cases, sympatric geographical distribution of babesia canis and babesia vogeli in dogs and associated vectors, in europe, the development of time-effici ... | 2017 | 28739301 |
| a pcr survey of vector-borne pathogens in different dog populations from turkey. | in the present study, a total of 192 blood samples were collected from pet dogs, kennel dogs and shepherd dogs in konya district, turkey, and tested by specific pcr for the presence of vector-borne pathogens. several pathogens were identified, most of which can cause substantial morbidity in dogs. pcr results revealed that 54 (28.1%) dogs were infected with one or more pathogens. positive results were obtained for babesia spp. in 4 dogs (2.1%), hepatozoon spp. in 8 dogs (4.2%) and mycoplasma spp ... | 2017 | 28682765 |
| molecular detection of vector-borne pathogens in dogs and cats from qatar. | vector-borne diseases (vbds) have been increasingly reported in dogs and cats worldwide. however, no data are currently available regarding canine and feline vbds in qatar and limited information is available from other persian gulf countries. | 2017 | 28633666 |
| molecular detection of tick-borne pathogens in wild red foxes (vulpes vulpes) from central italy. | spleen samples from 153 red foxes, shot during regular hunting season in the province of pisa (central italy), were examined to detect dna of anaplasma phagocytophilum, ehrlichia canis, coxiella burnetii, francisella tularensis, hepatozoon canis and babesia sp./theileria sp. dna of vector-borne pathogens was detected in 120 (78.43%; 95% ci: 71.06-84.66%) foxes. specifically, 75 (49%; 95% ci: 40.86-57.22%) animals scored pcr-positive per h. canis, 68 (44.44%; 95% ci: 36.42-52.69%) for e. canis, 3 ... | 2017 | 28502642 |
| seroprevalence and risk factors associated with ehrlichia canis, anaplasma spp., borrelia burgdorferi sensu lato, and d. immitis in hunting dogs from southern italy. | canine vector-borne diseases (cvbds) are caused by a range of pathogens transmitted to dogs by arthropods. the present study investigates ehrlichia canis, anaplasma spp., borrelia burgdorferi sensu lato, and dirofilaria immitis seroprevalences in hunting dogs from southern italy. dogs (no. 1335) were tested using a commercial in-clinic enzyme-linked immunosorbent assay kit. odds ratios (ors) were calculated by logistic regression analysis to identify risk factors. overall, 138/1335 dogs (10.3%) ... | 2017 | 28776227 |
| occurrence of <i>coxiella burnetii</i>, <i>ehrlichia canis</i>, <i>rickettsia</i> species and <i>anaplasma phagocytophilum</i>-like bacterium in ticks collected from dogs and cats in south africa. | ticks are major vectors of arthropod-borne infections and transmit a wide variety of zoonotic pathogens. this study was conducted mainly to determine the occurrence of canine tick-borne bacterial and rickettsial pathogens especially those with zoonotic potential. we examined 276 rhipicephalus sanguineus, 38 haemaphysalis elliptica and 4 amblyomma hebraeum ticks from 90 dogs and 4 cats from the free state, kwazulu-natal, north west and mpumalanga provinces. dna of coxiella burnetii (41%), ehrlich ... | 2017 | 28582983 |
| prevalence and geographic distribution of vector-borne pathogens in apparently healthy dogs in croatia. | vector-borne pathogens (vbps) are a group of globally extended and quickly spreading pathogens that are transmitted by various arthropod vectors. the aim of the present study was to investigate the seroprevalence against babesia canis, anaplasma phagocytophilum, borrelia burgdorferi sensu lato, leishmania infantum, dirofilaria immitis, and ehrlichia canis in dogs in croatia. we investigated 435 randomly selected apparently healthy dogs in 13 different locations of croatia for antibodies to b. ca ... | 2017 | 28448211 |
| molecular detection of tick-borne pathogen diversities in ticks from livestock and reptiles along the shores and adjacent islands of lake victoria and lake baringo, kenya. | although diverse tick-borne pathogens (tbps) are endemic to east africa, with recognized impact on human and livestock health, their diversity and specific interactions with tick and vertebrate host species remain poorly understood in the region. in particular, the role of reptiles in tbp epidemiology remains unknown, despite having been implicated with tbps of livestock among exported tortoises and lizards. understanding tbp ecologies, and the potential role of common reptiles, is critical for ... | 2017 | 28620610 |
| vector-borne pathogens in stray dogs in northeastern turkey. | this experiment was carried out to attain prevalence and molecular characterization of pathogens causing canine vector-borne diseases (cvbds) including babesiosis, hepatozoonosis, leishmaniasis, filariosis (dirofilaria immitis, dirofilaria repens, and acanthocheilonema reconditum), ehrlichiosis (ehrlichia canis), and anaplasmosis (anaplasma platys) in stray dogs. the study material consisted of 133 asymptomatic female (n = 96) and male (n = 37) stray dogs (≤1 year old, n = 16 and 1-6 years old, ... | 2017 | 28632488 |
| new wide dynamic range assays for quantification of anti-leishmania igg2 and iga antibodies in canine serum. | the aims of this study were (1) to develop and validate time resolved-immunofluorometric assays for the detection of anti-leishmania igg2 and iga antibodies in canine serum and (2) to evaluate the ability of these assays to quantify different amounts of anti-leishmania antibodies in leishmania-seronegative and seropositive dogs, determined by a commercial elisa assay, and between different clinical stages according to leishvet guidelines. the analytical validation showed that the assays had a go ... | 2017 | 28669382 |
| implication, clinical and biological impact of vector-borne haemopathogens in anaemic dogs in france: a prospective study. | to prospectively describe the clinical and biological impact of vector-borne haemopathogens in anaemic dogs in france and occurrence of haemolysis. | 2017 | 28543264 |
| molecular survey of anaplasma platys and ehrlichia canis in dogs from campo grande, mato grosso do sul, brazil. | this study investigated the frequency of infection by anaplasma platys and ehrlichia canis in dogs submitted to animal health centers in campo grande, state of mato grosso do sul, brazil. e. canis and a. platys showed infection frequencies of 55.75% and 16.96%, respectively. the identity of the two species was confirmed by dna sequencing. | 2017 | 28423085 |
| seropositivity for rickettsia spp. and ehrlichia spp. in the human population of mato grosso, central-western brazil. | the epidemiology of rickettsia and ehrlichia species infection is underestimated in mato grosso state. | 2017 | 28700062 |
| molecular prevalence and haemato-biochemical profile of canine monocytic ehrlichiosis in dogs in and around hisar, haryana, india. | the present study was planned to investigate the molecular prevalence of canine monocytic ehrlichiosis (cme) in dogs in and around hisar and to evaluate the haemato-biochemical profile for its better management. a total of 60 dogs presented to medicine section, tvcc, luvas, hisar with the history of naturally acquired tick infestation and clinical signs consistent with cme were screened on the basis of blood smear examination, followed by molecular detection by nested pcr assay targeting a porti ... | 2017 | 28848253 |
| cerebral haemorrhage as a clinical manifestation of human ehrlichiosis. | a 16-year-old young man presented to the emergency room with new-onset generalised tonic-clonic seizures. examination showed a glasgow score of 13 and predominantly crural left hemiparesis. imaging demonstrated a right frontoparietal haemorrhage of non-vascular origin with perilesional oedema. surgical drainage was carried out, but rebleeding occurred within 24 hours following surgery, and again 1 week after discharge. on reinterrogation and examination, ehrlichia canis infection was suspected a ... | 2017 | 28751428 |
| high-dose filgrastim treatment of nonregenerative pancytopenia associated with chronic canine ehrlichiosis. | to report the management and outcome of a dog with canine monocytic ehrlichiosis and nonregenerative pancytopenia, with high doses of filgrastim. | 2017 | 28750787 |
| ehrlichia canis and rickettsia spp. in dogs from urban areas in paraiba state, northeastern brazil. | the aims of our study was to identify ehrlichia canis and antibodies against rickettsia spp. belonging to the spotted fever group (sfg) in dogs sampled from paraiba state, northeastern brazil. blood and serum samples collected by convenience from dogs in urban areas of five municipalities were analyzed by real-time pcr for the detection of e. canis dna and by immunofluorescence assay test (ifat) for the identification of antibodies against rickettsia rickettsii, r. felis, r. parkeri, r. amblyomm ... | 2017 | 28658415 |
| therapeutic management of canine ehrlichiosis with aid of blood transfusion: a case report. | an adult female labrador retriever dog was presented at referral veterinary polyclinic, ivri in lateral recumbency with history of anorexia, high fever, severe panting, heavy tick infestation, and not responding to cephalosporins and fluoroquinolones since last 1 week. clinical examination showed pale conjunctiva, enlarged popliteal lymph node however, electrocardiogram (ecg) report found to be normal. haematological finding showed marked anaemia and thrombocytopenia. parasitological examination ... | 2017 | 28615848 |
| occurrence of leishmania infantum in the central nervous system of naturally infected dogs: parasite load, viability, co-infections and histological alterations. | zoonotic visceral leishmaniasis is caused by the protozoan leishmania infantum and little is known about the occurrence and pathogenesis of this parasite in the cns. the aims of this study were to evaluate the occurrence, viability and load of l. infantum in the cns, and to identify the neurological histological alterations associated with this protozoan and its co-infections in naturally infected dogs. forty-eight leishmania-seropositive dogs from which l. infantum was isolated after necropsy w ... | 2017 | 28419136 |