| human plasma angiotensinogen: a review of purification procedures. | the current status of the purification and characterization of human angiotensinogen is reviewed. one problem encountered in the past has been the copurification of a protein with similar porperties. this protein has tentatively been designated alanine-protein. an efficient separation of angiotensinogen and alanine-protein was obtained on a zinc chelate column. alanine-protein has been purified and its amino acid and carbohydrate composition determined. the cooh-terminal amino acid and the nh2-t ... | 1979 | 390363 |
| [preservation of cholerogenicity in cholera vibrios in the incomplete l-cycle stage]. | the work presents the characteristics of the forms appearing as a result of the incomplete l cycle of vibrio cholerae obtained in experimental conditions after the cultivation of typical v. eltor strain, serovar ogawa, for 5 months at room temperature in sterile river water without subculturing. the culture formed shaprly changed l-similar colonies, had decreased agglutinability and was resistant to diagnostic cholera phages, but retained its cholerogenicity for suckling rabbits. a suggestion wa ... | 1979 | 390934 |
| [comparative evaluation of the functional activity of different immunoglobulin classes in cholera]. | the immunological activity of specific immunoglobulins in different forms of cholera infection was studied with the use of the indirect immunofluorescent method. the forms of cholera infection were shown to be related to the physico-chemical spectrum of antibodies: patients with the pronounced clinical symptoms of cholera produced serum igm, including antigen -- antibody complex, more actively and in a greater amount; patients with faintly pronounced and asymptomatic infection produce highly act ... | 1979 | 390941 |
| natural premature protein synthesis termination can be reduced in escherichia coli by decreased translation rates. | peptidyl trna hydrolase is an essential enzyme for normal growth inasmuch as a mutant strain of escherichia coli with a temperature-sensitive hydrolase cannot continue protein synthesis at the non-permissive temperature. in the absence of hydrolase peptidyl trna rapidly accumulates. why peptidyl trna should be formed is the subject of this report. the rapid rate of protein synthesis is likely one mechanism of formation of peptidyl trna. a stra mutant of the hydrolase (pth-1) mutant strain that h ... | 1979 | 392230 |
| lung compliance and lung morphology following artificial ventilation in the premature and full-term rabbit neonate. | rabbit neonates delivered on day 27--30 of gestation were subjected to intermittent positive pressure ventilation (ippv) for 60 min. tidal volume was standardized to 10 ml/kg, and the insufflation pressure required to maintain this tidal volume was recorded. the quasistatic compliance of the lung-thorax system, corrected for variations in body weight, was significantly lower in animals delivered on day 27--28 than in those delivered on day 29--30. immature animals with low lung-thorax complianc ... | 1979 | 394314 |
| a simple laboratory method for the diagnosis of v. cholerae. | in many developing countries where cholera occurs, bacteriological facilities at the local level are lacking and either cholera may be unreported or other diarrhoeal diseases may be mistakenly reported as cholera. a simple and practical method, suitable for use in small hospitals and health centres and by field teams of health workers is described. swab samples are streaked on to various media (ttga and tcbs were found to be the most satisfactory), incubated overnight at 35 degrees c and read. t ... | 1979 | 394411 |
| the possible involvement of seagulls (larus sp) in the transmission of salmonella in dairy cattle. | a series of infections in a herd of dairy cows by different salmonella serotypes over a seven year period is described. the source of infection appeared to be the private water supply contaminated by seagulls. | 1979 | 394468 |
| genetic susceptibility to cholera. | in the course of studies of immunity to experimental cholera in man, 10(5) or 10(6) vibrio cholerae were given to 66 college students and other community volunteers under quarantine in an isolation ward. hla antigen and blood group determinations were carried out to test the hypothesis that severity of clinical cholera is dependent in part upon genetically-determined host susceptibility. fifty-five volunteers developed diarrhoea; 38 had mild illness and 17 had severe cholera (stool volume greate ... | 1979 | 394667 |
| local immune response in mice to vibrio cholerae. | cholera immunization schedules were investigated in mice, with emphasis placed on obtaining an immune response in the intestine. the most effective schedule for producing a good local response was found to be several orally-given priming doses of the organism followed after 14 days by an intravenous boosting dose. major differences between the immune responses in the spleen and the intestine were noted. | 1979 | 394733 |
| genetics of toxinogenesis in vibrio cholerae and escherichia coli. | | 1979 | 394927 |
| [the pathogenic and pathophysiologic aspects of the vibrio cholerae infection and of some other enterotoxicogenic acute diarrheal diseases (author's transl)]. | | 1979 | 395402 |
| [the molecular biology of aging -- 15: structure and function of the genetic apparatus in aging. ii. synthesis and metabolism of rna]. | with aging in different organs there are similar or tissue specific changes of the ribonucleic acids in respect of the quantity, the spectrum of different rna species and the metabolism of rna. among these processes the template function of the chromatin and the behaviour of the rna polymerases are important influencing factors. in aging the sensitivity of the rna synthesis against hormonal stimulation is decreased in the most cases. a survey about the results of many in vivo and in vitro experi ... | 1979 | 395770 |
| modes of action of enterotoxins from vibrio cholerae and escherichia coli. | current information on the intestinal secretory mechanism and on the actions of the enterotoxins from vibrio cholerae and escherichia coli are reviewed. these enterotoxins, through their effects on the metabolism of cyclic nucleotides, both inhibit active absorption and stimulate active secretion of water and electrolytes in the small intestine. the enterotoxin of v. chol erae and the heat-labile enterotoxin of e. coli affect these activities by stimulating adenylate cyclase, and the heat-stable ... | 1979 | 399384 |
| [anti-endotoxin therapy in liver diseases]. | | 1979 | 400139 |
| selective electrode for dibenzyl dimethyl ammonium cation as indicator of the membrane potential in biological systems. | the electrode sensitive to dibenzyl dimethyl ammonium (dda+), which is considered to be an indicator of the membrane potential, was constructed by using tetraphenyl borone (tpb-) embedded in dichloroethane. rapid and nernstian responses were exhibited against dda+ solutions ranging between 10(-2) and 3 - 10(-6) m in concentration. high selectivity for dda+ was observed in the presence of various inorganis salts, adp, atp, oxidizable substrates and sugars. the electrode developed here was used to ... | 1977 | 402152 |
| the role of cellular locomotion in leukemic infiltration. an organ culture study on penetration of l 5222 rat leukemia cells into the chick embryo mesonephros. | the significance of cellular locomotion for leukemic infiltration was investigated using l 5222 rat leukemia cells. previous cinemicrographic studies have shown that these cells are able to locomote only after formation of a uropod-like posterior extension. this characteristic locomotive configuration of l 5222 cells is easily recognizable in scanning electron micrographs and appropriate sections. leukemia cells were inoculated on slices of chick embryo mesonephros incubated for 24h; at this tim ... | 1977 | 403674 |
| naturally occurring human antibodies with specificity for light chains of immunoglobulins. | human sera of different categories were screened for antibodies against iga. a surprisingly high incidence of antibodies was observed. all of these antibodies were of restricted specificity, i.e. able to react with one or a few individual igas from a panel of eight myeloma proteins used in the screening. in one serum of a patient with selective iga deficiency an antibody against the allotype a2m(1) was found. some of the antibodies were shown to belong to the igg class. the iga, igg and igm cont ... | 1977 | 403678 |
| characterization of a chondroitin sulphate proteoglycan from ovine brain. | | 1977 | 404396 |
| neurite induction in mature cortical neurones in feline gm1-ganglioside storage disease. | | 1977 | 404568 |
| [effect of orally administered pancreatic extract on vibrio cholerae infection during protein deficiency]. | two groups of 5 and 6 vervet monkeys respectively were fed a protein-free diet. both groups of protein depleted vervets developed a minor decrease of serum and intestinal immunoglobulins, as well as marked impairment of exocrine pancreatic function. thereafter the groups were challenged with vibrio cholerae, while one group received purified pancreatic extract by oral administration. the group without pancreatic extract developed severe and significantly longer lasting diarrhea than the group wi ... | 1977 | 404703 |
| tissue reactions to long-term electrical stimulation of the cerebellum in monkeys. | light and electron microscopic analyses were carried out on the stimulated and unstimulated paravermal cortices of six rhesus monkeys that had electrodes implanted on their cerebella for 2 months. the electrodes and the stimulation regime (10 p.p.s.: 8 min on, 8 min off) were similar to those used to stimulate the human cerebellum for treatment of certain neurological disorders. mere presence of the electrode array in the posterior fossa for 2 months resulted in some meningeal thickening, attenu ... | 1977 | 408468 |
| detection and differentiation of iron-responsive avirulent mutants on congo red agar. | agar medium containing congo red dye differentiates virulent and avirulent colonies of shigella, vibrio cholerae, escherichia coli, and neisseria meningitidis. like virulent plague bacilli, wild-type cells of these species absorb the dye and produce red colonies. mutants or colonial variants have been isolated that fail to absorb the dye and produce colorless colonies. these mutants exhibit reduced virulence in the chicken embryo model, but their virulence is enhanced by supplementation with iro ... | 1977 | 409688 |
| application of the indirect enzyme-labeled antibody microtest to the detection and surveillance of animal diseases. | the rapid, indirect enzyme-labeled antibody (ela) microplate test has been developed as a diagnostic and surveillance tool to aid in the control of animal disease. the test has been applied to viral (hog cholera), parasitic (trichinosis), and bacterial (brucellosis) diseases of animals. a correlation of greater than 95% was observed between the hog cholera ela test and the serum neutralization test for hog cholera in greater than 2,000 field samples obtained during the 1976 epizootic in new jers ... | 1977 | 409789 |
| [immunogenicity of l5178y cells modified by different reagents]. | lymphoma l5178y cells were treated with neuraminidase of vibrio cholerae, potassium iodine, dithiotreitol (dtt), mercaptoethanol, glutaraldehyde, iodoacetamide, merthiolate, sodium periodate, urea, papaine, trypsine and edta, to increase immunoreaction in tumor cells. mice were immunized with modified tumor cells every week for one month. thereafter non modified tumor cells were transplanted to previously immunized mice. only the immunization with neuraminidase-treated cells rejected the tumor. ... | 1977 | 410377 |
| a placental view of the diagnosis and pathogenesis of congenital listeriosis. | | 1977 | 411374 |
| [use of the enzyme neuraminidase for immunotherapeutic treatment of chemically induced carcinogenesis]. | the effect of vibrio cholerae neuraminidase (vcn) on the growth of dimethylbenzanthracene-induced sarcoma cells in the inbred cba mice was investigated. the use of this preparation was started after the appearance of the tumour. injection of 50 units of vcn twice a week for three months was effective at the early stages of carcinogenesis. an increase of the life-span of mice of comparison with control animals was also observed in the animals inoculated intraperitoneally with induced syngeneic sa ... | 1977 | 413595 |
| the mechanism of complement fixation by rabbit f(ab')2: properties of the bactericidal reaction. | | 1977 | 413785 |
| [effect of a sulfur-containing nucleotide-peptide complex on the growth and development of different species of algae]. | | 1977 | 414054 |
| molecular cloning and in vitro transcription of bacillus subtilis plasmid in escherichia coli. | a composite plasmid (pat2010) has been constructed in vitro from rsf2124 and bacillus subtilis ifo3022 plasmid (pat1060) by covalent joining of the two dna molecules by means of escherichia coli dna ligase through the cohesive ends generated by restriction endonuclease ri (ecori) cleavage. the composite plasmid was selected by transformation of e. colic600r-m- with the ligated mixture after enrichment for composite plasmid by preparative agarose gel electrophoresis, and plating of the transforma ... | 1977 | 414072 |
| [problems of cancer immunotherapy (author's transl)]. | active immunization has received a new impulse most recently through the treatment of tumor cells with the enzyme neuraminidase from vibrio cholerae. there is no passive specific immunotherapy in humans. in the field of unspecific active immunotherapy there are very many, partly contradictory, findings, especially with bcg, corynebacterium parvum and preparations from these and other organisms. a cellularly transmitted immunity has been attempted, but so far without very encouraging results. it ... | 1977 | 414081 |
| [cephradine in urology]. | | 1977 | 414092 |
| open-field behavior after intravenous amphetamine analogues in rats. | a variety of behaviors were studied in an open-field setting after i.v. amphetamine (0.5, 2.0, 8.0 mg/kg), phenmetrazine (1.0, 4.0, 16.0 mg/kg), or fenfluramine (1.0, 4.0, 16.0 mg/kg). amphetamine and phenmetrazine increased ambulation initially and rearing during the whole experiment, and decreased grooming. at 30 and 60 min, with the three higher doses of amphetamine, stereotyped behaviors interfered with and decreased both ambulation and groomin. fenfluramine decreased ambulation, rearing, an ... | 1977 | 414270 |
| the use of tetracyclines on the chemotherapy of experimental east coast fever (theileria parva infection of cattle). | in a series of experiments, groups of cattle, undergoing patent east coast fever (ecf) reactions induced by the inoculation of tick-derived stabilates of theileria parva infective particles, were treated by parenteral administration of 2 tetracyclines. ten cattle were treated with n-pyrrolidinomethyl tetracycline at 15 mg/kg for 5 days, starting on the first day macroschizonts were detected in lymph node biopsy smears. nine of these treated cattle survived ecf reactions induced by an inoculum wh ... | 1977 | 414390 |
| [forssman's heterogenetic antigen and its demonstration in sheep muscle tissue]. | preparation of forssman's antiserum by means of hyperimmunization of rabbits with multiple injection of guinea-pig kidney emulsion i.v. in the course of 36 days is presented in the paper. the titre of the antiserum of rabbits utilized varied in the range between 1:1600 and 1:3200, using the antiserum to prove forssman's antigen. on the basis of the sachs-georgi reaction, the presence of the forssman's heterogenous antigen was recorded in all samples of meat collected from different parts of the ... | 1977 | 414406 |
| [sensitivity of meningococci to the antagonistic activity of nasopharyngeal microflora and antibiotics]. | comparative study of the meningococcus sensitivity of various serological groups with different localization in the human organism to the antagonistic activity of normal microbes of the nasopharynx and the antibiotics it was found that strains isolated from the cerebrospinal fluid of patients suffering from cerebrospinal meningitis, chiefly of serological group a, had the greatest resistance to the antagonists. taking into consideration the leading epidemiological role of the cultures belonging ... | 1977 | 414490 |
| prolactin responsiveness to trh in amenorrheic women with and without galactorrhea. | sixty women were given intravenous injection of 200 microgram trh to assess its diagnostic potential as a stimulus to prl release. following the administration of trh, there was a prompt increase in serum prl to 614.6%, to 296%, to 282.1%, and 34% in normal women, amenorrheic patients, non tumoral galactorrheic cases, and patients with pituitary tumors respectively. the trh response above baseline of prl levels was statistically significant in all groups, but the women with pituitary tumors whic ... | 1977 | 414510 |
| effects of eustachian tube ligation on auditory nerve responses to clicks. | auditory nerve responses to condensation and rarefaction clicks were recorded from the external ear canal of cats using a closed acoustic system. repeated control recordings from both ears formed a baseline for each of four animals used in this study. after a baseline had been established, the eustachian tube on one side was ligated and serial recordings of n1 responses were performed for up to 140 days postligation. by comparing the shift which occurred in the n1 latency-vs-click level plots af ... | 1978 | 414648 |
| glycosylation of endogenous lipids and proteins by preparations of chicken embryo fibroblasts. | glycosylation of endogenous phosphoisoprenyl lipids and membrane-associated proteins was shown to occur in preparations of chicken embryo fibroblasts incubated with gdp[14c]mannose and udp-n-acetylglucosamine. the two preparations used were cells released from the culture dishes by buffered saline containing edta and crude membranes from those cells. both beta-mannosyl-phosphoryldolichol and oligosaccharide-phosphoryl lipids with five to eight sugar residues were labelled under the conditons emp ... | 1978 | 414785 |
| mumps virus cytopathic effect in bonnet monkey kidney cell cultures. | | 1977 | 414999 |
| determination of corynebacterium diphtheriae toxigenicity by a colorimetric tissue culture assay. | chinese hamster ovary (cho) cell cultures in microtiter wells are sensitive to growth inhibition and killing by picogram quantities of diphtheria toxin. in the absence of biologically active toxin, the cho cell culture produces sufficient acidic metabolites to change the phenol red ph indicator from pink to yellow within 56 h. in the presence of 10 pg of toxin per well, growth inhibition can be observed microscopically within 24 h. diphtheria toxin can be qualitatively assayed from culture super ... | 1978 | 415068 |
| the effects of severe keratitis on corneal transmission. | an investigation into the effects of keratitis on corneal light transmission is presented. transmission data from 9 diseased corneas are compared to those of 22 normal corneas in the 0.3-0.8 micron spectral range. rhesus monkey corneas were used in the study. several color photographs illustrate varieties of idiopathic keratitis in rhesus monkeys. | 1978 | 415084 |
| diencephalic efflux of calcium ions in the monkey during exercise, thermal stress and feeding. | 1. the diencephalon of the unanaesthetized macaque monkey was radio-labelled with calcium by a microinjection of 6-8 muc (45)ca(2+) into the third cerebral ventricle through a permanently implanted cannula. successive 5 min push-pull perfusions of the mid-line hypothalamic region with an artificial c.s.f. were carried out at a rate of 28 mul./min every 20 min. a washout curve of declining (45)ca(2+) radioactivity was thus generated.2. when the monkey exercised strenuously on a special ;rowing ma ... | 1977 | 415131 |
| isolation of a bacillus subtilis 168 derivative sensitive to defective bacteriophage pbsx. | a derivative of bacillus subtilis strain 168 sensitive to defective bacteriophages pbsx and pbsz has been isolated. this particular strain, rub824, carries the genetic information for defective bacteriophage pbsx. | 1978 | 415153 |
| [nonspecific infection in patients with tuberculosis of the urinary tract during antibiotic therapy]. | | 1977 | 415177 |
| respiratory syncytial virus infection in adults: clinical, virologic, and serial pulmonary function studies. | we prospectively studied 10 previously healthy adults who developed an acute respiratory illness while working in an infants' ward during a community outbreak of respiratory syncytial virus infection. in addition to clinical and viral evaluation, total respiratory resistance before and after carbachol aerosol inhalation was measured. all 10 subjects had respiratory syncytial virus infection documented by viral isolation, and all developed pronounced cough, nasal congestion, and fever. eight subj ... | 1978 | 415653 |
| connective tissue composition of aortas from non-human primates. a comparative study. | connective tissue composition of aortas from several non-human primate species has been studied in an effort to relate collagen, elastin, ang glycosaminoglycan (gag) content to species susceptibility to atherosclerosis. among the species studied the baboon contained the highest content of gag in the aorta. while the distribution of individual gag varied from species to species, heparan sulfate (hs) was the highest gag in aortas from most of the species. the ratio of hs to chondroitin sulfates (c ... | 1978 | 415748 |
| slow virus e malattie croniche del sistema nervoso centrale. | the purpose of this report was to detect whether some subacute or chronic human neurological diseases are significantly associated with traditional virus like papovavirus and paramixovirus and non conventional agents (scrapie-like) or non classified (multiple sclerosis associated agent or msaa). in this connection for m.s. the data concerning the ethiological factors paramixovirus or mssa are presented and discussed and it is hypothesized the virus mechanism in the pathogenesis of m.s. | 1978 | 415749 |
| effect of age, weight and adequacy of zinc intake on the balance between alkaline ribonuclease and ribonuclease inhibitor in various tissues of the rat. | 1. deficiency of zinc inhibits growth and also increases the activity of alkaline ribonuclease in certain tissues of the rat (prasad & oberleas, 1973). zn could influence ribonuclease activity by direct effects on the enzyme or its natural inhibitor, or non-specifically as occurs when growth rate is affected by various other factors. these possibilities were studied. 2. alkaline ribonuclease was shown to be inhibited by zn in vitro, but the concentrations of zn required were so high that the enz ... | 1978 | 415757 |
| evaluation of a surgical method for immobilizing the eye of an alert monkey. | one eye of a macaque monkey was immobilized by severing the third, fourth, and sixth cranial nerves. the residual movements of the operated eye were measured by repeatedly mapping the position of a visual receptive field. movements of several degrees were caused by contraction of the orbital musculature during the closure blink. the amount of movement was dramatically reduced by paralysis of the superficial orbital muscles with a local anesthetic. side effects of surgical immobilization include ... | 1978 | 415895 |
| production and quality control of therapeutically applicable vibrio cholerae neuraminidase (vcn). | | 1977 | 415923 |
| effect of oral pancreatic enzymes on the course of cholera in protein-deficient vervet monkeys. | the effect of pancreatic extract on the course of cholera infection in protein-deficient vervet monkeys (cercopithecus aethiops) was studied. eleven animals were made protein deficient by diet. thier intestinal pancreatic enzyme concentration decreased as they became protein depleted. these animals were then challenged with vibrio cholerae. four control animals given a standard diet were similarly challenged. immediately after challenge, 6 of the protein-deficient monkeys were given a highly pur ... | 1978 | 415928 |
| brucellosis in elk i. serologic and bacteriologic survey in wyoming. | incidence of brucellosis in elk (cervus canadensis) on two winter feedgrounds in wyoming was examined over a 5-year period by testing serum samples using the standard plate agglutination (spt) buffered brucella antigen (bba), rivanol (riv) and complement fixation (cft) tests. thirty-one percent of 1,165 elk were positive by defined criteria. considering each test individually, only 29% (106) of 370 positive sera would have been classified as reactors by the spt, 83% (307) by the bba test and 86% ... | 1978 | 416232 |
| hypercomplexities in the visual cortex. | | 1978 | 416357 |
| [carbon polymer stomata in vesicostomy and other urinary diversion procedures (author's transl)]. | implants of pure carbon are ideal for prosteses because of their tissue acceptability and lack of incrustation. animal experiments with biocarbon implants were tested as subfascial implantations, coeco-, ileo-, and vesicostomies, (n = 37). the results showed lack of reaction and good healing of the stomata, lack of incrustation, and water tight urinary diversion. based on these animal experiments biocarbon vesicostomies were carried out successfully in six patients. the follow up period was 3--1 ... | 1979 | 419640 |
| lesions of the myofibril in myopathic and normal tissue from seven striated muscles. experimental myopathy of isometric activity produced by phencyclidine and restraint. | | 1979 | 419941 |
| the effect of cancer on the spin-lattice relaxation time of mouse blood and tissue. | spin-lattice relaxation time measurements were made on the muscle, heart, kidneys, spleen, liver, brain and blood of both normal mice and those injected with sarcoma-180. as well as a marked increase in the relaxation time of the tumour itself, mice injected with sarcoma-180 showed a rise in the kidney relaxation time, along with blood relaxation times which were often either above or below the range found in normal mice. correlation coefficients for the relaxation times of the various organs we ... | 1979 | 420868 |
| effect of partial hepatectomy and hydrocortisone administration on liver and serum sialyltransferase activities. | partial hepatectomy of rats was followed by a rise in liver sialyltransferase activity. the maximum (2.5-fold increase) was reached on the third day after the operation, after which the level started to decline, returning to normal by day 6. determination of serum sialyltransferase in these animals showed a parallel pattern. daily injection of 5 mg hydrocortisone to adrenalectomized rats led to a maximal 3-fold elevation in liver sialyltransferase within 3 days, but failed to elicit any change i ... | 1979 | 420869 |
| cadmium accumulation and metabolism by rat liver parenchymal cells in primary monolayer culture. | primary cultures of adult rat liver parenchymal cells, isolated by the collagenase perfusion technique and maintained as a monolayer, were used to investigate the characteristics of hepatic cadmium accumulation and metabolism. cadmium accumulation was found to be a temperature- and concentration-dependent process that required sulfhydryl groups and was significantly stimulated by the addition of dexamethasone to the medium. once taken up, cadmium was less available for exit-exchange processes th ... | 1979 | 420870 |
| cold stress influence on lung lecithin in the newborn rabbit. | term, food-water deprived newborn rabbits exposed to a cold environment for 48 h demonstrated a significant decrease in total lung lipid (p less than 0.01), total triglyceride (p less than 0.001), total phospholipid (p less than 0.05), and total phosphatidylcholine (p less than 0.025). disaturated phosphatidylcholine remained unchanged. fatty acid methyl esters of total and disaturated phosphatidylcholine were not influenced by cold stress. likewise, there was no alteration in pulmonary function ... | 1979 | 420891 |
| [effect of hypotensive agents on baroreceptor reflexes in waking animals]. | | 1979 | 420939 |
| fatigue of human polymorphonuclear leukocyte function associated with bacterial ingestion. | | 1979 | 421375 |
| the urinary tract image obtained during 99mtc bone scanning. | during the course of a technetium (99mtc) bone scan, it is usual to obtain an image of both kidneys and the bladder. a review of 96 bone scans performed over a two-year period was undertaken in an attempt to assess the accuracy of this image. ten examinations showed abnormalities of the urinary image suitable for inclusion in the paper, and were subsequently further investigated. three cases showed that relatively small lesions may be detected within a kidney if localised caliectasis produced a ... | 1979 | 421427 |
| the ultrastructure of red nuclei neurons in the valine-deficient rat. | | 1979 | 421863 |
| [effect of lithium hydroxybutyrate on several heart function indices]. | dl50 of intraperitoneal lithium oxybutyrate administered to cats was found to amount to 724 mg/kg, while in doses of 13.5--360 mg/kg it increases the coronary blood flow volume in cats by 20.3--122.2 per cent, directly proportional to the dose. lithium oxybutyrate prevents the chlorocalcium arrhythmia in rats and eliminates strophanthine-induced anemia in cats, surpassing an analogous effect of lithium chloride, potassium chloride, quinidine, novocainamide and isoptin. it raises the activity of ... | 1979 | 421889 |
| hospital and physicians operated competing cat scanners. | | 1979 | 422175 |
| depression of cell-mediated immunity in cholera. | cholera toxin may depress cell-mediated immunity by stimulation of adenyl cyclase and production of cyclic amp in cellular systems or when given parenterally to experimental animals. whether or not similar effects might be found during clinical infection with vibrio cholerae was the subject of this study. delayed hypersensitivity reactions to skin test antigens were found to be markedly depressed in bengali patients with cholera 24 h after fluid repletion. skin test response rates were lower in ... | 1979 | 422232 |
| differentiation in normal human buccal mucosa epithelium. | the epithelium of normal human buccal mucosa was studied by light and electron microscopy and stereological procedures in twenty punch biopsies of 10-15 years old females. morphometric point counting techniques were employed in order to estimate the density of cell membranes in the superficial third of this epithelium. observations and data suggested that (1) there is a dense and regular papillary body of slender connective tissue papillae which bifurcate within and penetrate the basal two third ... | 1979 | 422483 |
| restoration of specific myxovirus receptors to asialoerythrocytes by incorporation of sialic acid with pure sialyltransferases. | | 1979 | 422571 |
| virus-neutralizing antibody in sera and secretions of the upper and lower respiratory tract of chickens inoculated with live and inactivated newcastle disease virus. | | 1979 | 422769 |
| significance of levels of specific immunoglobulins to dna in sle patients' sera detected by solid phase radioimmunoassay. | a solid phase radioimmunoassay was developed for detecting the quantity of double-stranded and single-stranded dna antibodies in patients with systemic lupus erythematosus and other connective tissue diseases. the assay system employs a solid support 96-well, flex-vinyl microtiter plate to which bovine methyl albumin is layered, followed by denatured or native calf thymus dna. a 1:80 dilution of patients' sera was added to respective wells followed by tritiated high affinity anti-igg, -iga, or i ... | 1979 | 422867 |
| stability of estrogen receptors in frozen human breast tumor tissue. | the effect of freezing on the stability of the estrogen receptors in human breast tumors was measured. portions of three tumors kept frozen for 3 days after removal from the patients were analyzed by the sucrose density gradient method. the initial measurements demonstrated positive receptor values. the remaining portions were kept frozen for 30 days, and the receptor values were reevaluated. the 8s receptor was 80% destroyed under these freezing conditions. the 4s receptor was found to be more ... | 1979 | 427754 |
| isolation and immunochemical characterization of antibodies from the sera of cancer patients which are reactive against human melanoma cell membranes by affinity chromatography. | immunoglobulins were isolated by affinity chromatography from sera of two patients with melanoma, one with sarcoma, and one with carcinoma. the affinity columns were prepared by covalently linking the membrane-rich fraction of biopsied melanoma cells to cyanogen bromide-activated agarose beads. the membrane-rich fractions were prepared by two methods: (a) hypotonic cell lysis, and (b) homogenization and differential centrifugation. melanoma sera were autologous to melanoma membrane preparations. ... | 1979 | 427806 |
| structural studies of the vibrio cholerae o-antigen. | | 1979 | 427831 |
| effect of metal ions on the production of vascular permeability factor by 569b strain of vibrio cholerae. | | 1979 | 429022 |
| the structure and amount of tubulin in cells and tissues. | the nh2-terminals and amino acid compositions of three carboxymethylcysteine-containing peptides produced from carboxymethylated calf brain tubulin by digestion with elastase have been determined. the 3h/14c ratio obtained when these peptides were generated from mixtures of [14c]carboxymethylated calf brain tubulin and the [3h]carboxymethylated proteins of mouse brain or 3t3 cells and subsequently purified indicated that both mouse brain and 3t3 cells contained protein giving rise to peptides ho ... | 1979 | 429276 |
| two strains of vibrio species with unusual biochemical features isolated from ear tracts. | a strain of vibrio cholerae heiberg type ii, not agglutinable with any of the eight antisera corresponding to heiberg's groups, and a nonmotile, methyl red-positivs of chronic external otitis. | 1979 | 429536 |
| comparison of four plating media for isolating vibrio cholerae. | two brands of thiosulfate citrate bile salts sucrose agar and monsur taurocholate tellurite gelatin (ttg) agar were compared with two newly developed media, sucrose tellurite teepol agar and vibrio parahaemolyticus agar for isolation and identification of vibrio cholerae. the thiosulfate citrate bile salts sucrose and ttg agars were the best selective media, whereas sucrose tellurite teepol agar was the poorest. both ttg and sucrose tellurite teepol agars were good for use in follow-up serologic ... | 1979 | 429540 |
| a rapid test for the identification of all serotypes of vibrio cholerae (including "non-agglutinating" vibrios). | | 1979 | 430547 |
| effect of central venous pressure on arterial baroreflex control of heart rate. | there is considerable evidence that the level of afferent cardiopulmonary receptor activity modulates sinus node responses to arterial baroreflex stimulation in experimental animals. we tested the hypothesis that this reflex interaction occurs also in man by measuring sinus node responses to arterial baroreceptor stimulation with phenylephrine injection or neck suction, before and during changes of central venous pressure provoked by lower body negative pressure or leg and lower trunk elevation. ... | 1979 | 434172 |
| ovine placental vascular responses to indomethacin. | the administration of 10 mg/kg indomethacin to chronically catheterized near-term sheep resulted in a change in cotyledonary vascular resistance from 0.133 +/- 0.016 to 0.180 +/- 0.022 (mmhg . min)/ml (p less than 0.001). the noncotyledonary uterine vascular resistance increased to 191% of the original value (p less than 0.001) and the renal vascular resistance increased to 147% of the control value (p less than 0.001). the maternal blood pressure increased from 96 +/- 3.4 to 108 +/- 3.4 mmhg (p ... | 1979 | 434175 |
| primary structure of cholera toxin subunit a1: isolation, partial sequences and alignment of the brcn fragments. | | 1979 | 437113 |
| fatty acid composition of lipopolysaccharides of vibrio cholerae 35a3 (inaba), nib 90 (ogawa), and 4715 (nag). | considerable amounts of odd-numbered fatty acids, such as non-hydroxy c15 and c17 and 3-hydroxy c11 and c13 acids, were found in lipopolysaccharides from vibrio cholerae 35a3 (inaba). | 1979 | 438134 |
| unmasking of actual and potential receptor sites for cholera toxin in intestinal mucosal homogenates. | endongenous and exogenous sialidases appear to unmask sialidase-stable and sialidase-labile gangliosides in intestinal mucosal homogenates by attacking glycoproteins. exogenous (but not endogenous) sialidase then converts sialidase-labile gangliosides into the cholera toxin-binding, sialidase-stable ganglioside gm1 (galactosyl-n-acetylgalactosaminyl [sialosyl] lactosyl ceramide). since vibrio cholerae produces sialidase, these observations may be relevant to the course of cholera. | 1979 | 438537 |
| contribution of adrenal medulla, spleen and lymph, to the plasma levels of dopamine beta-hydroxylase and catecholamines induced by hemorrhagic hypotension in dogs. | | 1979 | 438992 |
| [t-wave]. | | 1979 | 441545 |
| cholera: the present world situation. | | 1979 | 442165 |
| immunity of cholera in man: relative role of antibacterial versus antitoxic immunity. | purified cholera toxoid is antigenic when given enterally and orally. purified toxoid fails to provide protection against experimental challenge. clinical cholera confers formidable protection against homologous or heterologous rechallenge. failure to culture vibrios from intestinal fluid or stool of re-challenge volunteers suggests that the predominant immune mechanism is antibacterial rather than antitoxic. | 1979 | 442179 |
| persistence of methylated bases in ribonucleic acid of syrian golden hamster liver after administration of dimethylnitrosamine. | 1. syrian golden hamster liver ribosomal rna was isolated up to 96 h after administration of [14c]dimethylnitrosamine at 25 mg/kg or 2.5 mg/kg body weight. 2. the chemical alkyation products, 7-methylguanine, 3-methylcytosine, o6-methylguanosine and 1-methyladenosine, were measured after acidic or enzymic hydrolysis of the rna to bases or mononucleosides followed by ion-exchange chromatography. 3. between 7 and 96 h, the relative amounts of alkylation products did not change with time even thoug ... | 1979 | 444213 |
| variations in the sialic acid compositions in glycoproteins of mouse ascites tumor cell surfaces. | | 1979 | 444445 |
| adjuvant immunotherapy of malignant melanoma. | a vaccine made of irradiated vibrio cholerae neuroaminidase (vcn) treated autochthonous tumor cells plus bcg was utilized in combination with surgery or with chemotherapy for stage ii and stage iii malignant melanoma, respectively. a few patients with stage i melanoma were treated with surgery and bcg. most of the studies were carried out on a prospective, randomized protocol. when the results with conventional therapy were compared with the results of conventional therapy plus immunotherapy, no ... | 1979 | 445331 |
| immunotherapeutic response of concanavalin a-bound l1210 vaccine enhanced by a streptococcal immunopotentiator, ok-432. | immunotherapeutic response to concanavalin a (con a)-bound l1210 murine leukemic vaccine and immunopotentiators was examined in histocompatible animals bearing a small burden l1210 leukemic cells. when combined with con a-bound vaccine, a streptococcal immunopotentiator, ok-432 (nsc b116209), prepared from streptococcus pyogenes, was potent in antitumor therapy and resulted in a number of cured animals. administration of either con-a-bound vaccine or ok-432 alone did not produce any beneficial e ... | 1979 | 445486 |
| expanded linkage map of vibrio cholerae. | an expanded linkage map of the vibrio cholerae classical strain 162 chromosome has been prepared using a variety of new auxotrophic mutants. the chromosome consists of a single, linear linkage group. the map consists of 17 markers, which have been ordered; 20 mutational sites, which are tentatively ordered; five markers (ura-1, ser-2, mal-1, man-1, suc-1), which are linked but unordered; and three mutations (aro-2, cys-2 and cys-6) which showed little or no linkage. a proposal is made to standar ... | 1979 | 447068 |
| enhancement of protection against experimental cholera in rabbits by prior immunization of bcg along with vibrio cholerae. | | 1979 | 447377 |
| enzymic activity of cholera toxin. i. new method of assay and the mechanism of adp-ribosyl transfer. | we tested various methods of assaying the adp-ribosyltransferase activity of cholera toxin using artificial acceptors of the adp-ribosyl group. any of several proteins or poly(l-arginine) could be used with [adenine-14c]nad+ as adp-ribosyl donor, but this method was not ideal because of the heterogeneity of potential acceptor groups and the necessity of using costly labeled nad+. we, therefore, developed an alternative assay using a synthetic low molecular weight acceptor, 125i-n-guanyltyramine ... | 1979 | 447682 |
| carbohydrate components of influenza c virions. | the carbohydrate components of influenza c virions grown in chicken kidney (ck) cells were analyzed by gel filtration following exhaustive digestion with pronase. the [(3)h]glucosamine-labeled glycopeptides were larger and more heterogeneous than those of influenza a/wsn virions; three major size classes (g(1), g(2), and g(3)) were resolved. treatment with vibrio cholerae neuraminidase caused a decrease in size of g(1) and g(2), along with release of about 16% of the (3)h label. the released sug ... | 1979 | 448803 |
| noradrenergic innervation of cerebral cortex: widespread effects of local cortical lesions. | the trajectory of the intracortical noradrenergic fibers has been characterized by histochemical analysis following the production of cortical lesions in the rate. a large group of noradrenergic fibers enters the cortex at the frontal pole and proceeds caudally through the deep layers of dorsolateral cortex. branches arise from these longitudinally directed fibers and form a uniform pattern of innervation throughout lateral cortex. because these fibers travel long distances rostrocaudally within ... | 1979 | 451605 |
| flavobacterium meningosepticum infections. | | 1979 | 451641 |
| [possibility of purifying cholera toxin (choleragen) and its antibodies by an immunosorption method]. | a study was made to establish the possibility of using an immune adsorbent based on polyacrylamide gel with the concentration of t = 10% and c = 25% for the purification of cholerogen and antibodies to it. for the isolation of cholera toxin the gamma globulin fraction of antitoxic serum was incorporated into the adsorbent; for obtaining antibodies an adsorbent with partially purified cholerogen was used. desorption was made with 3 m sodium rhodanide solution. the yield of cholerogen was 41%, and ... | 1979 | 452780 |
| phagocytic peripheral blood monocytes from rabbits and humans express membrane receptors specific for igm molecules: evidence that incubation with neuraminidase exposes cryptic igm (fc) receptors. | phagocytic human and rabbit peripheral blood monocytes, identified by their ingestion of polystyrene particles, were investigated for the presence of surface membrane receptors for igm molecules. after incubation of freshly isolated monocytes with igm anti-sheep erythrocyte (srbc) preparations, a mean of 0.7% of human monocytes and a mean of 16.2% of rabbit monocytes formed rosettes with srbc. however, if the monocytes were pre-incubated with vibrio cholerae neuraminidase (vcn), these figures in ... | 1979 | 455787 |
| a study of el tor vibrios from the cases of gastroenteritis. | | 1979 | 457216 |