| [visualization of forensically important foreign bodies by direct roentgen magnification]. | the detection of foreign bodies of microscopic dimensions with radiographic techniques is limited by the size and density of the particle and the radiographic resolution. these restrictions can be of decisive value in case work. the high grade direct radiographic magnification technique was successfully used in preceding experiments. due to its 1 mu focal spot, this tube allows direct magnifications up to 250 times. in present studies, the advantages as well as the limitations of this new techni ... | 1989 | 2818477 |
| reaction of tryptophanyl-trna synthetase from beef pancreas with periodate-oxidized atp. | tryptophanyl-trna synthetase from beef pancreas reacts with periodate-oxidized atp according to biphasic kinetics. a rapid phase involves two groups of the protein, presumably lysine side-chains. the slow phase corresponds to the reaction of a larger number of groups. the time-course of the partial losses of the atp-ppi isotopic exchange and of the aminoacylation activities of the enzyme follow the labelling of the two fast-reacting groups. however, the ability of the enzyme to form a bis(trypto ... | 1987 | 2820730 |
| antibodies to polymerized human serum albumin in acute and chronic liver disease. | since antibodies to polyalbumin have been noted to occur in patients with hepatitis or cirrhosis, we investigated sera from 219 patients with a variety of acute and chronic liver diseases with and without hbsag using an elisa. the prevalence of circulating polyalbumin antibodies was shown to be significantly increased over that of healthy controls (6.7%) in patients with autoimmune chronic active hepatitis (67%), acute viral hepatitis (41%) and fulminant hepatic necrosis (38%), but such antibodi ... | 1987 | 2820857 |
| antibodies to polymerized human serum albumin in acute and chronic liver disease. | since antibodies to polyalbumin have been noted to occur in patients with hepatitis or cirrhosis, we investigated sera from 219 patients with a variety of acute and chronic liver diseases with and without hbsag using an elisa. the prevalence of circulating polyalbumin antibodies was shown to be significantly increased over that of healthy controls (6.7%) in patients with autoimmune chronic active hepatitis (67%), acute viral hepatitis (41%) and fulminant hepatic necrosis (38%), but such antibodi ... | 1987 | 2820857 |
| [wiedemann-beckwith syndrome: clinical characteristics, constitutional chromosome abnormalities and tumor incidence]. | the observation of constitutional chromosome abnormalities in a one and half year old boy with wiedemann-beckwith syndrome (wbs) and hepatoblastoma prompted us to review the currently available literature dealing with this subject. in approximately 500 published cases with complete and incomplete forms of wbs, 42 malignant and 9 benign neoplasms have been described. malignant tumors, in particular, mainly consisted of nephroblastomas and adrenocortical carcinomas. the patient presented in this p ... | 1987 | 2821320 |
| quantitative analysis of protein mixtures by second derivative absorption spectroscopy. | a new method for the quantitative analysis of protein mixtures based on multicomponent analysis of the second derivative near uv spectra is described. using bovine eye lens crystallins, we demonstrate that the technique can provide precise concentrations of closely related proteins within mixtures, under both native and denaturing conditions. we have also successfully used the method to analyze the subunit composition of a heteromultimeric protein aggregate. the method is more rapid and precise ... | 1989 | 2817383 |
| [functional morphology of the maxillo-mandibular apparatus in the miniature swine mini-lewe. 11. discussion of the findings of the metric studies and dentition (conclusion)]. | the graphs showing the dimensions and angles of the skull and jaw indicate that postnatal development trends are basically uniform. comparison with the vietnamese miniature pig shows that the miniature pig mini-lewe has a longer skull. postnatal skull growth is accompanied by a distinct shift in proportions. the sequence of dentition if broadly identical to that of the domestic pig and the pitmann-moore miniature pig. | 1989 | 2817410 |
| use of nude (athymic) mice for the study of hypertrophic scars and keloids: vascular continuity between mouse and implants. | hypertrophic scars and keloids appear to be unique to humans since animals are not known to form these lesions. therefore, in an effort to develop an experimental model for their study, implants of these human lesions were made in nude (athymic) mice (nu/nu) in suprascapular subcutaneous pockets. the implants were recovered from 2 to 246 days. by histological and fine structural parameters all implants remained viable and their morphological character was maintained. selected mice were injected ... | 1989 | 2817436 |
| studies on the immunoglobulin v kappa locus in human lymphoid cell lines. | dna digests of 16 human lymphoid cell lines were studied in blot hybridization experiments with probes from v kappa genes and their immediate neighborhood as well as with single or low-copy probes from intergenic regions. the patterns were compared with those of placenta dna digests in which the kappa genes are in the germline configuration. the differences of patterns which were detected with the first type of hybridization probes can be attributed to v kappa--j kappa rearrangements or to restr ... | 1987 | 2822060 |
| repeated sequences of 3-kb dna fragment of a plasmid from yersinia enterocolitica 09 in plasmids from y. pestis and y. pseudotuberculosis. | | 1987 | 2822355 |
| mobilization of the vwa plasmid of yersinia pestis by f-containing strains of escherichia coli. | | 1987 | 2822356 |
| virological implications of the use of primates in xenotransplantation. | | 1987 | 2823433 |
| virological implications of the use of primates in xenotransplantation. | | 1987 | 2823433 |
| [evaluation of the modulating action of yersinia pestis adenylate cyclase on guinea pig peritoneal leukocytes using chemiluminescence]. | the biological action of y. pestis adenylate cyclase on peritoneal leukocytes of guinea pigs has been studied by means of chemiluminescence. y. pestis adenylate cyclase is supposed to contribute to the "oxidation" explosion of phagocytes in plague. | 1987 | 2823508 |
| [cyclic nucleotides in the dynamic development of shock caused by the murine toxin of yersinia pestis]. | the authors have studied the effect of y. pestis "mouse" toxin (ld50), injected intravenously to rats, on camp and cgmp content in the tissues of different organs (the lungs, liver, heart, spleen, kidneys, small intestine) and in the blood in the course of the development of toxinfection shock. the effect of y. pestis "mouse" toxin on cyclic nucleotide content in the organs of experimental animals is determined by the sum of oppositely directed effects produced by the thermostable and thermolabi ... | 1987 | 2823509 |
| near-infrared spectrophotometric monitoring of haemoglobin and cytochrome a, a3 in situ. | | 1987 | 2823549 |
| near-infrared spectrophotometric monitoring of haemoglobin and cytochrome a, a3 in situ. | | 1987 | 2823549 |
| spontaneously regressive canine cutaneous histiocytoma: a counterpart of human regressing atypical histiocytosis? | | 1987 | 2823685 |
| experimental transmission and pathogenesis of immunodeficiency syndrome in cats. | we describe the identification, experimental transmission, and pathogenesis of a naturally occurring powerfully immunosuppressive isolate of feline leukemia virus (designated here as felv-faids) which induces fatal acquired immunodeficiency syndrome (aids) in 100% (25 of 25) of persistently viremic experimentally infected specific pathogen-free (spf) cats after predictable survival periods ranging from less than 3 months (acute immunodeficiency syndrome) to greater than one year (chronic immunod ... | 1987 | 2823940 |
| experimental transmission and pathogenesis of immunodeficiency syndrome in cats. | we describe the identification, experimental transmission, and pathogenesis of a naturally occurring powerfully immunosuppressive isolate of feline leukemia virus (designated here as felv-faids) which induces fatal acquired immunodeficiency syndrome (aids) in 100% (25 of 25) of persistently viremic experimentally infected specific pathogen-free (spf) cats after predictable survival periods ranging from less than 3 months (acute immunodeficiency syndrome) to greater than one year (chronic immunod ... | 1987 | 2823940 |
| evidence for histidyl and carboxy groups at the active site of the human placental na+-h+ exchanger. | the na+-h+ exchanger of the human placental brush-border membrane was inhibited by pretreatment of the membrane vesicles with a histidyl-group-specific reagent, diethyl pyrocarbonate and with a carboxy-group-specific reagent, n-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline. in both cases the inhibition was irreversible and non-competitive in nature. but, if the membrane vesicles were treated with these reagents in the presence of amiloride, cimetidine or clonidine, there was no inhibition. since ... | 1987 | 2822022 |
| chronic administration of sertraline, a selective serotonin uptake inhibitor, decreased the density of beta-adrenergic receptors in rat frontoparietal cortex. | sertraline, a potent and selective inhibitor of serotonin uptake, was chronically administered to laboratory rats. using in vitro receptor autoradiographic techniques, we found that the binding of [3h]dihydroalprenolol ([3h]dha) was reduced in cortex layers iv-vi. results of a saturation experiment indicated that the reduction in cortex layer iv was due to a change in number but not affinity of beta-adrenergic receptors. | 1987 | 2825912 |
| chronic administration of sertraline, a selective serotonin uptake inhibitor, decreased the density of beta-adrenergic receptors in rat frontoparietal cortex. | sertraline, a potent and selective inhibitor of serotonin uptake, was chronically administered to laboratory rats. using in vitro receptor autoradiographic techniques, we found that the binding of [3h]dihydroalprenolol ([3h]dha) was reduced in cortex layers iv-vi. results of a saturation experiment indicated that the reduction in cortex layer iv was due to a change in number but not affinity of beta-adrenergic receptors. | 1987 | 2825912 |
| effects of caffeine and cyclic adenosine 3',5'-monophosphate on adenosine triphosphate-dependent calcium uptake by lysed brain synaptosomes. | the adenosine triphosphate-dependent calcium uptake by endoplasmic reticulum elements of lysed synaptosomes from rat brain cortex was studied. caffeine exhibited a biphasic effect on this calcium uptake activity: concentrations of 1, 2, 5, 10 or 30 mm caffeine stimulated calcium uptake by 62, 111, 73, 88 and 60% respectively, whereas calcium uptake was inhibited by 55% at a 60-mm concentration of caffeine. calcium release from endoplasmic reticulum elements of lysed brain synaptosomes was stimul ... | 1987 | 2825917 |
| protein kinase c inhibitors eliminate hippocampal long-term potentiation. | recent findings suggest that protein kinase c (pkc) regulates the persistence of long-term potentiation (ltp). to test the hypothesis that pkc inhibition would decrease persistence of potentiation we applied pkc inhibitors (mellitin, polymyxin b, h-7) by micropressure ejection to the intact hippocampus either before or after ltp induction. when inhibitor was given 15 min before ltp, initial potentiation was unaffected, yet responses decayed to baseline levels by 60 min after the onset of potenti ... | 1987 | 2825923 |
| agonist-dependent phosphorylation of the alpha 2-adrenergic receptor by the beta-adrenergic receptor kinase. | desensitization of the beta-adrenergic receptor, a receptor which is coupled to the stimulation of adenylate cyclase, may be regulated via phosphorylation by a unique protein kinase. this recently discovered enzyme, known as the beta-adrenergic receptor kinase, only phosphorylates the agonist-occupied form of the beta-adrenergic receptor. to assess whether receptors coupled to the inhibition of adenylate cyclase might also be substrates, we examined the effects of beta-adrenergic receptor kinase ... | 1987 | 2826414 |
| mucosal antibody response of calves after oral and intrabronchial administration of rotavirus. | | 1987 | 2827448 |
| herpes simplex virus infection of the hand. a profile of 79 cases. | seventy-nine cases of herpes simplex (hsv) infection of the hand were documented by viral culture over a 40-month period. a marked bimodal age distribution was found with 32 cases occurring in adults aged 21 to 30 years and 16 occurring in children less than 10 years old. the infection most commonly occurred on the fingers (69 percent) and thumb (21 percent). all 20 herpes simplex type 2 (hsv-2) isolates came from patients over 20 years old. all of the 13 specimens that were typed after isolatio ... | 1988 | 2827469 |
| evidence for histidyl and carboxy groups at the active site of the human placental na+-h+ exchanger. | the na+-h+ exchanger of the human placental brush-border membrane was inhibited by pretreatment of the membrane vesicles with a histidyl-group-specific reagent, diethyl pyrocarbonate and with a carboxy-group-specific reagent, n-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline. in both cases the inhibition was irreversible and non-competitive in nature. but, if the membrane vesicles were treated with these reagents in the presence of amiloride, cimetidine or clonidine, there was no inhibition. since ... | 1987 | 2822022 |
| guanine nucleotide regulation of adenylate cyclase in permeabilized cells of saccharomyces cerevisiae. | adenylate cyclase in permeabilized cells of saccharomyces cerevisiae was examined. among various permeabilization procedures, including organic solvents, detergents and other reagents, dimethylsulfoxide (dmso) and digitonin treatments resulted in the highest recovery of adenylate cyclase activity. incubation of cells at 30 degrees c with digitonin at 0.01% to 0.1%, or dmso at 20% to 40% for 15 to 30 min gave optimal adenylate cyclase activity. the enzyme activity in digitonin-permeabilized cells ... | 1988 | 2830910 |
| molecular cloning and characterization of an rrna operon in streptomyces lividans tk21. | the number of rrna genes in streptomyces lividans was examined by southern hybridization. randomly labeled 23 and 16s rrnas were hybridized with bamhi, bglii, psti, sali, or xhoi digests of s. lividans tk21 dna. bamhi, bglii, sali and xhoi digests yielded six radioactive bands each for the 23 and 16s rrnas, whereas psti digests gave one band for the 23s rrna and one high-intensity band and six low-density bands for the 16s rrna. the 7.4-kilobase-pair bamhi fragment containing one of the rrna gen ... | 1988 | 2832372 |
| genetic analysis of the iron uptake region of the vibrio anguillarum plasmid pjm1: molecular cloning of genetic determinants encoding a novel trans activator of siderophore biosynthesis. | clones carrying the iron uptake region of the vibrio anguillarum plasmid pjm1 were subjected to insertion mutagenesis, using transposon tn3::hoho1 which carries a promoterless lacz gene and can thus generate lacz transcriptional fusions if inserted downstream from an indigenous promoter. four classes of insertion mutants were obtained based on the level of expression of components of the iron uptake system, and six genetic units were defined according to the phenotype of the mutants. five of the ... | 1988 | 2832388 |
| genetic analysis of the iron uptake region of the vibrio anguillarum plasmid pjm1: molecular cloning of genetic determinants encoding a novel trans activator of siderophore biosynthesis. | clones carrying the iron uptake region of the vibrio anguillarum plasmid pjm1 were subjected to insertion mutagenesis, using transposon tn3::hoho1 which carries a promoterless lacz gene and can thus generate lacz transcriptional fusions if inserted downstream from an indigenous promoter. four classes of insertion mutants were obtained based on the level of expression of components of the iron uptake system, and six genetic units were defined according to the phenotype of the mutants. five of the ... | 1988 | 2832388 |
| epigenetic control and reintegration of extrachromosomal proviral dna in hl60 cells chronically infected with human t cell leukaemia virus type 1. | extrachromosomal human t cell leukaemia virus type 1 (htlv-i) proviruses are persistently maintained in htlv-i-infected human promyelocytic leukaemia (hl60) cells even 24 months after viral infection. by successive recloning of these htlv-i-infected clones, and by southern blot analysis of their htlv-i proviruses, we concluded the following. the copy number of extrachromosomal proviruses fluctuated, and this fluctuation was probably dependent on the epigenetic conditions in the host cell, hl60. ... | 1988 | 2832528 |
| viruses in sewage waters during and after a poliomyelitis outbreak and subsequent nationwide oral poliovirus vaccination campaign in finland. | during an outbreak of paralytic poliomyelitis in finland in 1984 and 1985 the widespread circulation of the causative wild-type serotype 3 poliovirus in the population was documented by demonstrating the virus in sewage water specimens in 13 different locations in the greater helsinki district and in 13 other cities or towns all over the country. after the nationwide campaign with oral poliovirus vaccine in 1985, poliovirus serotypes 2 and 3 were readily isolated from sewage waters for up to 2 m ... | 1988 | 2833160 |
| widespread occurrence of the restriction endonuclease yeni, an isoschizomer of psti, in yersinia enterocolitica serotype o8. | the cold-active restriction endonuclease yeni, an isoschizomer of psti, was found in 12 of 14 yersinia enterocolitica serotype o8 strains of different origins, but not in other serotypes of y. enterocolitica, yersinia pseudotuberculosis, or yersinia pestis. in spite of the limited number of strains tested, the result suggests that the detection of yeni endonuclease or the gene might result in more rapid determination of the prominently pathogenic serotype of y. enterocolitica. | 1988 | 2833162 |
| studies on the immunoglobulin v kappa locus in human lymphoid cell lines. | dna digests of 16 human lymphoid cell lines were studied in blot hybridization experiments with probes from v kappa genes and their immediate neighborhood as well as with single or low-copy probes from intergenic regions. the patterns were compared with those of placenta dna digests in which the kappa genes are in the germline configuration. the differences of patterns which were detected with the first type of hybridization probes can be attributed to v kappa--j kappa rearrangements or to restr ... | 1987 | 2822060 |
| structural and functional domains of the myb oncogene: requirements for nuclear transport, myeloid transformation, and colony formation. | the v-myb oncogene of avian myeloblastosis virus causes acute myelomonocytic leukemia in vivo and transforms only myeloid cells in vitro. its product, p48v-myb, is a nuclear protein of unknown function. to determine structure-function relationships for this protein, we constructed a series of deletion mutants of v-myb, expressed them in retroviral vectors, and studied their biochemical and biological properties. we used these mutants to identify two separate domains of p48v-myb which had distinc ... | 1988 | 2835503 |
| a multiple end-point approach to evaluation of cytotoxicity and genotoxicity of erythrosine (fd and c red no. 3) in a v79 hepatocyte-mediated mutation assay. | v79 chinese hamster lung cells were used to evaluate in vitro the cytotoxicity and genotoxicity of erythrosine (2', 4', 5', 7'-tetraiodofluorescein disodium salt; fd and c red no. 3), a color additive used widely in foods, drugs and cosmetics. erythrosine reduced colony size at 200 micrograms/ml and was lethal to 90% or more of the cells at 400 micrograms/ml. at dose levels of 100, 200 and 300 micrograms/ml of medium, erythrosine was non-mutagenic to v79 cells at the hypoxanthine-guanine phospho ... | 1988 | 2835676 |
| effects of gossypol on the activity of kidney (na+ + k+)-atpase and the functions of erythrocyte membrane. | gossypol extracted from cottonseed oil, reputed to exert contraceptive action in males, may induce a side effect of hypokalemia. the cellular and molecular mechanisms by which gossypol produces hypokalemia are not quite understood. we have examined the inhibitory effect of gossypol on the activity of (na+ + k+)-atpase purified from the outer medulla of rabbit kidney, the half maximal inhibitory concentration (ic50) is 6.5 micron. the kinetic studies using this enzyme preparation show that gossyp ... | 1988 | 2836126 |
| serological survey for selected diseases in the endangered san joaquin kit fox (vulpes macrotis mutica). | blood from endangered san joaquin kit foxes (vulpes macrotis mutica) inhabiting the elk hills naval petroleum reserve, kern county, and the elkhorn plain, san luis obispo county, california, was collected in 1981, 1982 and 1984 and sera were tested for antibodies against 10 selected pathogens. proportions of kit fox sera containing antibodies against pathogens were: canine parvovirus, 100% in 1981-1982 and 67% in 1984; infectious canine hepatitis virus, 6% in 1981-1982 and 21% in 1984; canine di ... | 1988 | 2836636 |
| efficacy of the neuropeptide org.2766 in the prevention and treatment of cisplatin-induced neurotoxicity in rats. | in rats chronic systemic treatment with cisplatin results in a sensory neuropathy as evidenced by a reduction in the sensory conduction velocity in the sciatic nerve. concomitant administration of the neurotrophic acth4-9 analog, org.2766, prevents the occurrence of the neuropathy. in addition, treatment with org.2766 stops further deterioration and improves recovery of an already established cisplatin-induced neuropathy. furthermore, concomitant administration of org.2766 during a first cisplat ... | 1988 | 2838292 |
| efficacy of the neuropeptide org.2766 in the prevention and treatment of cisplatin-induced neurotoxicity in rats. | in rats chronic systemic treatment with cisplatin results in a sensory neuropathy as evidenced by a reduction in the sensory conduction velocity in the sciatic nerve. concomitant administration of the neurotrophic acth4-9 analog, org.2766, prevents the occurrence of the neuropathy. in addition, treatment with org.2766 stops further deterioration and improves recovery of an already established cisplatin-induced neuropathy. furthermore, concomitant administration of org.2766 during a first cisplat ... | 1988 | 2838292 |
| [incompatibility of the cad plasmid from yersinia pestis with plasmids of the incfi group]. | the relation of yersinia pestis calcium dependence plasmid (pcad) to known inc fi (f'lac, r386, pox38) and incfv (f0lac) plasmids has been studied. evidence that plasmid pcad of yersinia pestis belongs to fi incompatibility group is presented. | 1987 | 2828935 |
| patch-clamp recording of amino acid-activated responses in "organotypic" slice cultures. | patch-clamp recording techniques were used to study the properties of amino acid-activated channels in cultured "organotypic" slices from rat cerebellum and hippocampus. hippocampal pyramidal cells responded to the three main glutamatergic agonists, n-methyl-d-aspartate (n-me-d-asp), quisqualate, and kainate, whereas purkinje cells responded only to quisqualate and kainate. analysis of single-channel events recorded in outside-out patches from hippocampal neurons showed large conductance events ... | 1988 | 2834737 |
| comparative investigation of pmn leucocyte antimicrobial potential in animals of different species susceptibility to the plague agent. | the present work was aimed at a comparative study of oxygen-dependent metabolism (om) and myeloperoxidase (mpo) activities as well as the content of bactericidal cationic proteins (bcp) in polymorphonuclear leucocytes (pmnl) from mammals and birds with different susceptibility to plague (mice, guinea pigs, rats, great and miday gerbils, rabbits, dogs, pigeons and man). the experimental finding of an association between infectious susceptibility and functioning of the major pmnl bactericidal syst ... | 1987 | 2824603 |
| preparation of heteroenzyme conjugates: trypsin-chymotrypsin and trypsin-alkaline phosphatase. | hybrid enzymes which have two different enzyme activities linked together covalently may be useful reagents for various applications, such as the determination of complex biological structures. the present paper describes the preparation and purification of two such enzyme-enzyme conjugates, namely, trypsin-chymotrypsin and trypsin-alkaline phosphatase. whereas the former has been prepared by using the well-known bifunctional reagent glutaraldehyde, the latter exploited the schiff base formation ... | 1988 | 2840096 |
| [studies on the hypothalamus-hypophyseal corticoliberin system. iii. effect of long-term administration of corticoliberin on serum levels of acth, tsh and thyroxine in male rats]. | | 1987 | 2842125 |
| the structure of the human skin fibroblast collagenase gene. | genomic clones containing the complete gene encoding human fibroblast interstitial collagenase were isolated from a lambda phage human dna library. the gene is comprised from 10 exons and spans 8.2 kilobase pairs. we have mapped the relative positions and determined the dna sequence of all the exon/intron borders of the gene. the organization of the human interstitial collagenase gene is very similar to that of rabbit collagenase and of two other extracellular matrix (ecm) metalloproteases: rat ... | 1988 | 2839503 |
| the structure of the human skin fibroblast collagenase gene. | genomic clones containing the complete gene encoding human fibroblast interstitial collagenase were isolated from a lambda phage human dna library. the gene is comprised from 10 exons and spans 8.2 kilobase pairs. we have mapped the relative positions and determined the dna sequence of all the exon/intron borders of the gene. the organization of the human interstitial collagenase gene is very similar to that of rabbit collagenase and of two other extracellular matrix (ecm) metalloproteases: rat ... | 1988 | 2839503 |
| detection of leukotriene b4 in cardiac tissue and its role in infarct extension through leucocyte migration. | the main left coronary artery of rats was ligated near its origin under light ether anaesthesia and the infarction observed for 48 h. the ischaemic area was determined after an intravenous injection of pontamine sky blue dye 1 h before induction of cardiac arrest with potassium chloride. the unstained area (true ischaemic area) decreased with time to 27.1% of the left ventricle at 48 h, whereas the intensely stained area between the normal and the true ischaemic areas increased with time, sugges ... | 1988 | 2844405 |
| efficacy of a combination thromboxane receptor antagonist and lipoxygenase inhibitor in traumatic shock. | the effects of a thromboxane receptor antagonist having lipoxygenase inhibitory activity, l-655,240 (3-[1-(4-chlorobenzyl)-5-fluoro-3-methyl-indol-2-yl]2,2-dimethylpropa noic acid) (1 mg/kg per h) were studied in a standardized model of traumatic shock. pentobarbital (35 mg/kg) anesthetized rats subjected to noble-collip drum trauma were characterized by a 82 +/- 12 min survival time, a 20-fold increase in plasma cathepsin d activity, and a 6-fold increase in plasma myocardial depressant factor ... | 1988 | 2845544 |
| efficacy of a combination thromboxane receptor antagonist and lipoxygenase inhibitor in traumatic shock. | the effects of a thromboxane receptor antagonist having lipoxygenase inhibitory activity, l-655,240 (3-[1-(4-chlorobenzyl)-5-fluoro-3-methyl-indol-2-yl]2,2-dimethylpropa noic acid) (1 mg/kg per h) were studied in a standardized model of traumatic shock. pentobarbital (35 mg/kg) anesthetized rats subjected to noble-collip drum trauma were characterized by a 82 +/- 12 min survival time, a 20-fold increase in plasma cathepsin d activity, and a 6-fold increase in plasma myocardial depressant factor ... | 1988 | 2845544 |
| heterogeneity of glycine receptors and their messenger rnas in rat brain and spinal cord. | messenger rnas isolated from adult or newborn rat spinal cord were fractionated in a sucrose gradient. the fractions were injected into xenopus oocytes to determine their potencies for expression of glycine receptors (glyrs), which were then examined electrophysiologically. the sedimentation profiles disclosed two classes of glyr mrnas, one heavy and the other light. the adult spinal cord was rich in heavy glyr mrna, whereas the light glyr mrna was more abundant in neonatal spinal cord and in ad ... | 1988 | 2845580 |
| characterization of vaccinia virus dna topoisomerase i expressed in escherichia coli. | the putative structural gene encoding the vaccinia virus type i dna topoisomerase (ec 5.99.1.2) was expressed in escherichia coli under the control of a bacteriophage t7 promoter. provision of t7 rna polymerase resulted in the accumulation to high level of a mr = 33,000 type i topoisomerase with the properties of the vaccinia enzyme. a simple purification scheme yielded approximately 8 mg of recombinant vaccinia topoisomerase from 400 ml of bacteria. dna unwinding by the enzyme was stimulated by ... | 1988 | 2846543 |
| characterization of vaccinia virus dna topoisomerase i expressed in escherichia coli. | the putative structural gene encoding the vaccinia virus type i dna topoisomerase (ec 5.99.1.2) was expressed in escherichia coli under the control of a bacteriophage t7 promoter. provision of t7 rna polymerase resulted in the accumulation to high level of a mr = 33,000 type i topoisomerase with the properties of the vaccinia enzyme. a simple purification scheme yielded approximately 8 mg of recombinant vaccinia topoisomerase from 400 ml of bacteria. dna unwinding by the enzyme was stimulated by ... | 1988 | 2846543 |
| infection control measures for human immunodeficiency virus (hiv): universal precautions. | | 1988 | 2846803 |
| [effect of bilirubin and serum albumin on the na,k-atpase activity in the synaptosomal membrane]. | the absorption spectrum of visible light, characteristic of the free bilirubin being in the aqueous medium, with a single maximum at 440 nm and with the shoulder in the region of 410-420 nm is transformed into the spectrum with two maxima in the region of 460 and 500 nm, respectively, when the pigment is bound in vitro by the synaptosomal membrane. there are two types of sites for bilirubin binding in the membrane particles, differing in the values of constants of association (ka = 0.6 . 10(5) a ... | 1988 | 2847380 |
| influence of immunomodulatory agents on bovine humoral and cellular immune responses to parenteral inoculation with bovine rotavirus vaccines. | sodium diethyldithiocarbamate (dtc), mycobacterium cell wall extract (mcwe, regressin), killed corynebacterium parvum (c. parvum, immunoregulin) and muramyldipeptide (mdp) were each combined with purified, live bovine rotavirus and inoculated into 3 month-old holstein-friesian calves in order to examine their ability to potentiate specific humoral and cellular immune responses. the vaccinated calves were boosted twice at 3 and 6 weeks after initial vaccine inoculation. the rotavirus was administ ... | 1988 | 2847397 |
| activities of amikacin, roxithromycin, and azithromycin alone or in combination with tumor necrosis factor against mycobacterium avium complex. | the effect of amikacin and two new macrolides (roxithromycin and azithromycin) used either alone or in combination with recombinant tumor necrosis factor (tnf) to inhibit or kill mycobacterium avium complex in human macrophages was examined in vitro. macrophage monolayers infected with m. avium complex (strain 101, serotype 1) were treated with antibiotics or tnf by using three different protocols: (i) antibiotics or tnf was added to the monolayers immediately after infection and washed out afte ... | 1988 | 2847644 |
| activities of amikacin, roxithromycin, and azithromycin alone or in combination with tumor necrosis factor against mycobacterium avium complex. | the effect of amikacin and two new macrolides (roxithromycin and azithromycin) used either alone or in combination with recombinant tumor necrosis factor (tnf) to inhibit or kill mycobacterium avium complex in human macrophages was examined in vitro. macrophage monolayers infected with m. avium complex (strain 101, serotype 1) were treated with antibiotics or tnf by using three different protocols: (i) antibiotics or tnf was added to the monolayers immediately after infection and washed out afte ... | 1988 | 2847644 |
| activation of calpain i and calpain ii: a comparative study using terbium as a fluorescent probe for calcium-binding sites. | the present study demonstrates the activation of calpain i and calpain ii by micromolar levels of terbium and has utilized the enhancement in the fluorescence of protein-bound terbium to study and compare the calcium binding sites of the two enzymes. calpain i and calpain ii were isolated from bovine erythrocytes and brain, respectively. while the rates of activation of calpain i by terbium and calcium are comparable, the rate of activation of calpain ii was much greater in the presence of terbi ... | 1988 | 2847653 |
| inhibition of porphyrin biosynthesis by exogenous 5-aminolevulinic acid in an aerobic photosynthetic bacterium, erythrobacter sp. och 114. | exogenously administered 5-aminolevulinic acid (ala) inhibited the formation of bacteriochlorophyll a (bchl a) in a dose-dependent manner in the aerobic photosynthetic bacterium, erythrobacter sp. strain och 114, under dark growth conditions. the ala concentration required for half-inhibition after 24-h growth was estimated to be about 3.0 mm. porphyrin and bchl precursors were not found in either the cells or the growth medium. the same inhibition was also observed with cytochrome c formation. ... | 1988 | 2847654 |
| human placental endonuclease cleaves holliday junctions. | a partially purified endonuclease from human placenta cleaves cruciform structures. the placental enzyme is active both on extruded cruciform structures from negatively supercoiled covalently closed circular plasmid dna and on synthetic x-junctions formed by reannealing short oligonucleotides. plasmids containing natural or cloned palindromes such as pbr322 and phd101-3 were used as substrates. the synthetic x-junction tetramer dna formed by reannealing short oligonucleotides, was converted into ... | 1988 | 2847712 |
| [effects of y. pestis mouse toxin on carbohydrate metabolism in rats]. | effects of intravenous y. pestis mouse toxin (ld50) injection on glucose, lactate glucagon, insulin blood levels and camp liver content in dynamics of intoxication development were studied. hypoglycemia, observed 2 hours after toxin administration seems not to be due to the enhanced glucose utilization in peripheral tissues because insulin blood level during this period was decreased and lactate concentration has not been changed. glucagon content by 2-5 hour of shock was strong elevated. propos ... | 1988 | 2847830 |
| dual control of the intracellular ph in aortic smooth muscle cells by a camp-sensitive hco3-/cl- antiporter and a protein kinase c-sensitive na+/h+ antiporter. | two mechanisms are involved in the regulation of the intracellular ph (phi) of aortic smooth muscle cells: the na+/h+ antiporter and a na+-independent hco3-/cl- antiporter. the na+/h+ antiporter acts as a cell alkalinizing mechanism. it is activated by vasopressin and by phorbol esters when cells are incubated in the presence of bicarbonate but is not affected in the absence of bicarbonate. the hco3-/cl- antiporter acts as a cell acidifying mechanism. agents such as forskolin, 8-br-camp, and iso ... | 1988 | 2848021 |
| cell-specific activity of the human immunodeficiency virus enhancer repeat in vitro. | the binding of nuclear proteins and the functional activity of the hiv-ltr enhancer repeats in different cell lines (jurkat, cem, h9, u937, raji, b cells, t47d, hela, 293, and hepg2 cells) was investigated in vitro. five distinct complexes formed with the enhancer repeat have been identified by an electrophoretic mobility shift assay. the distribution of these complexes varied qualitatively and quantitatively between nuclear proteins from different sources. in the extracts tested, transcription ... | 1988 | 2848558 |
| [human chorionic prealbumin: its identification, physicochemical properties and detection in the blood serum in trophoblastic disease]. | human chorionic prealbumin (hcpa) was immunochemically identified and isolated in pure form from immature placenta, particularly, from chorion. comparative immunodiffusion analysis shows that hcpa differs from the known placenta-specific proteins. hcpa is a glycoprotein with mw about 100 kd determined by gel-filtration and 45 kd by sds polyacrylamide gel electrophoresis. by immunodiffusion hcpa was not found in fetal and adult tissues and in biological fluids. hcpa was discovered in patients wit ... | 1988 | 2848598 |
| use of progress curves to estimate the co-substrate-to-substrate flow ratio of a symport mechanism. application to the isoleucine-na+ symport of mouse ascites-tumour cells and to the lactose-proton symport. | the model envisages two components in the process, whereby ht equivalents of co-substrate and st equivalents of substrate accumulate in the cellular compartment in time t. the first is the flow through the symport, n equivalents of co-substrate entering or leaving with each substrate equivalent. the second is the basal flow of co-substrate outside the symport. in certain specific circumstances n can be derived by plotting ht/t against st/t. the principal requirement is that, whereas the ratio of ... | 1988 | 2839154 |
| characterization of receptors for platelet-activating factor on platelets, polymorphonuclear leukocytes and macrophages. | 1. we have compared the potency of the putative platelet-activating factor (paf) receptor antagonists (web 2086, l-652,731 and bn 52021) against paf-induced aggregation of rabbit and guinea-pig platelets, aggregation of rabbit polymorphonuclear leukocytes (pmnls) and prostacyclin generation by guinea-pig resident peritoneal macrophages. 2. on rabbit washed platelets and pmnls web 2086, l-652,731 and bn 52021 each antagonized competitively paf-induced aggregation. the rank order of potency was we ... | 1988 | 2850058 |
| [changes in the cyclic nucleotide system of t-lymphocytes in experimental tuberculosis in animals]. | | 1988 | 2851783 |
| plasminogen activator/coagulase gene of yersinia pestis is responsible for degradation of plasmid-encoded outer membrane proteins. | the related family of virulence plasmids found in the three major pathogens of the genus yersinia all have the ability to encode a set of outer membrane proteins. in y. enterocolitica and y. pseudotuberculosis, these proteins are major constituents of the outer membrane when their synthesis is fully induced. in contrast, they have been difficult to detect in y. pestis. it has recently been established that y. pestis does synthesize these proteins, but that they are rapidly degraded due to some a ... | 1988 | 2843471 |
| [ridge augmentation with controlled porous structure hydroxyapatite (aps-7) granule in tooth extraction cavity]. | | 1988 | 2855431 |
| cytomegalovirus infection in fifty-two renal transplant recipients. | | 1987 | 2856277 |
| prostanoids and adrenaline release: a study of [3h]adrenaline efflux from the rabbit isolated, perfused, adrenal gland. | [3h]adrenaline was incorporated in an isolated perfused preparation of the rabbit adrenal gland and the effects of indomethacin, pge2 and pgi2 on its release were investigated. efflux of [3h]adrenaline was elicited by electrical stimulation of the splanchnic nerve (60 s at 5 hz). indomethacin (3 and 30 microm) had no effect on stimulation-induced efflux. pge2 (30, 90 and 300 nm) reduced the efflux; with 90 nm pge2 the inhibition amounted to approximately 30%. pgi2, in concentrations from 90 to 6 ... | 1985 | 2858566 |
| the role of diffusible molecules in regulating the cellular differentiation of dictyostelium discoideum. | a central problem in developmental biology is to understand how morphogenetic fields are created and how they act to direct regionalized cellular differentiation. this goal is being pursued in organisms as diverse as moulds, worms, flies, frogs and mice. each organism has evolved its own solution to the challenge of multicellularity but there appear to be common underlying principles and, once pattern formation is fully understood in any system, some general truths seem certain to be revealed. a ... | 1988 | 2848678 |
| genetic analysis of the 9.5-kilobase virulence plasmid of yersinia pestis. | the 9.5-kilobase plasmid of yersinia pestis determines plasminogen activator, coagulase, pesticin, and pesticin immunity activities. we have mapped and cloned the loci encoding these activities and demonstrated that both plasminogen activator and coagulase were determined by the same gene, designated pla. the primary translation product of this gene (38 kilodaltons [kda]) was processed in two sequential steps to produce peptides of 37 and 35 kda. the first step in this processing occurred rapidl ... | 1988 | 2843470 |
| lav/htlv-iii antibodies in connective tissue diseases. | | 1985 | 2863569 |
| combination of mefloquine with sulfadoxine-pyrimethamine compared with two sulfadoxine-pyrimethamine combinations in malaria chemoprophylaxis. | | 1985 | 2863679 |
| [adhesion pili in yersinia pestis]. | y. pestis cells cultivated at 37 degrees c are capable of agglutinating red blood cells of some animals, which is due to the appearance of pili. the adhesion pili consist of protein subunits with a molecular weight of the order of 12000 daltons, their isoionic point being at ph 4.7. the reaction of hemagglutination was inhibited by the mixture of ganglyosides, while the preliminary treatment of red blood cells with neuraminidases increased its effectiveness. the pili are supposed to take part in ... | 1985 | 2863905 |
| neurohumoral vasoconstrictor systems in heart failure. | by means of specific inhibitors of the renin-angiotensin system (captopril) and of the sympathetic nervous activity (prazosin) in dogs with congestive heart failure, and by using a specific antagonist of the pressor activity of arginine vasopressin in rats with heart failure, we studied the influence of these pressor hormone systems on peripheral vascular resistance and cardiac function. all three humoral vasoconstrictor systems were stimulated in heart failure. the experiments in dogs showed th ... | 1985 | 2864248 |
| characterization of depolarization induced by palytoxin and grayanotoxin-i in isolated cardiac tissues from dogs and guinea pigs. | the mode of depolarizing action of palytoxin (ptx) was compared with that of grayanotoxin-i (gtx-i) to examine the site of action of ptx in cardiac tissues isolated from dogs and guinea pigs: ptx at above 1 x 10(-10) mol/l depolarized the membrane of canine purkinje fibres, canine and guinea-pig ventricular muscles regardless of stimulation. the ptx-induced depolarization was resistant to 1 x 10(-5) mol/l tetrodotoxin (ttx) but was attenuated by low na medium. gtx-i at 1 x 10(-5) mol/l depolariz ... | 1985 | 2864640 |
| mechanism of tachycardia caused by intracarotid pge2 in conscious ewes. | conscious adult ewes prepared with nonocclusive indwelling vascular catheters were used to determine the mechanism by which heart rate increases during central administration of prostaglandin e2 (pge2). heart rate increased 14 bpm during steady-state intracarotid infusion of pge2, 10 ng/kg/min (p less than 0.05). intravenous atropine methyl bromide, 1 mg/kg, increased heart rate 26 bpm (p less than 0.05) 5 min after injection. heart rate remained elevated 30 min after injection. the heart rate r ... | 1985 | 2864696 |
| angiogenin. | | 1985 | 2866393 |
| induction of procollagen processing in fibroblast cultures by neutral polymers. | in cultures of dermal fibroblasts, procollagen and the intermediates pc- and pn-collagen accumulated in the culture medium with little further processing to collagen. when polyethylene glycol (peg) or other neutral polymers were added to fibroblast culture medium, no collagen or procollagen was found in the medium, but all the collagen was associated with the cell layer. the type i procollagen was fully processed to collagen with an initial transient accumulation of pn-collagen, and the processe ... | 1986 | 2869039 |
| [comparisons of vasoconstrictions induced by alpha agonists either in the luminal perfusate or in the adventitial superfusate]. | | 1985 | 2869558 |
| [phenomenon of pili formation in yersinia enterocolitica]. | in y. enterocolitica strain, serovar 0:10, the capacity for the formation of pili inducing the mannose-resistant hemagglutination (mrha) of formolated sheep red blood cells was due to the presence of plasmid pye10. mrha-inducing pili differed serologically from y. pestis and y. tuberculosis adhesion pili. plasmid pye10 was immobilized for transfer to cells of escherichia coli strain hb101 (rec a) by means of prp 4. the expression of mrha-inducing pili in the new host the rec a-independent charac ... | 1986 | 2871677 |
| the fine structure of atypical ciliated cells in the human gastric epithelium. | gastric mucosa obtained from the body and pyloric portions of the human stomach were observed by light and transmission electron microscopy. ciliated cells were found in two of 18 subjects examined, one patient with gastric ulcer and the other one with gastric adenocarcinoma. the ciliated cells were found in epithelia at sites away from the main lesions. the tissues containing ciliated cells showed intestinal metaplasia combined with mild chronic gastritis in both cases. the epithelial layer fac ... | 1986 | 2876543 |
| differential expression of cell surface antigens and glial fibrillary acidic protein in human astrocytoma subsets. | we have characterized five distinct cell surface antigens of human astrocytomas and correlated their expression with the expression of glial fibrillary acidic protein (gfap) and four previously defined cell surface markers of astrocytomas. one of the newly studied antigens, a4, which was originally detected on rat central nervous system (but not peripheral nervous system) neurons, is expressed on gfap+ human astrocytoma cells, but not on gfap- astrocytomas or a wide range of other neuroectoderma ... | 1986 | 2877731 |
| a bipotential neuroendocrine precursor whose choice of cell fate is determined by ngf and glucocorticoids. | adrenal medullary endocrine (chromaffin) cells and sympathetic neurons both derive from the neural crest. we have found that the embryonic adrenal medulla and sympathetic ganglia are both initially populated by precursors expressing neural-specific genes. by birth, however, the medulla consists largely of chromaffin cells. in primary culture, the medullary precursors have three developmental fates: in ngf they continue to mature into neurons and survive, whereas in glucocorticoid they either ext ... | 1986 | 2877748 |
| spatial distribution of langerhans' cells and t-lymphocyte subpopulations in human tympanic membrane and aural cholesteatoma. | immunohistochemical analysis of human cholesteatoma matrices revealed the presence of langerhans' cells and t-lymphocytes. through cell-to-cell interaction, langerhans' cells probably play a key role in skin-related disorders, including cholesteatomas. they probably originate from a mobile cell population of monocyte origin and migrate into and out of the body's lining. their custodial function is often carried out in close relation with t-lymphocytes. monoclonal antibodies against langerhans' c ... | 1986 | 2878530 |
| drug effects upon aqueous production. | | 1986 | 2879370 |
| human papillomavirus dna in bronchial squamous cell carcinomas. | | 1987 | 2880009 |
| avaii polymorphism in the human ldl receptor gene. | | 1987 | 2881256 |
| region-specific expression of two mouse homeo box genes. | mammalian homeo box genes have been identified on the basis of sequence homology to drosophila homeotic and segmentation genes. these studies examine the distribution of transcripts from two mouse homeo box genes, hox-2.1 and hox-3.1, throughout the latter third of prenatal development. transcripts from these genes are regionally localized along the rostro-caudal axis of the developing central nervous system, yielding expression patterns very similar to patterns of drosophila homeotic gene expre ... | 1987 | 2881353 |
| prolactin and its involvement in fluid regulation in the bovine. | plasma prolactin (prl) concentrations, osmolality, water consumption, feed intake, urine excretion, and fecal water output were determined in twelve steers of 3 breeds exposed to 5 feed and water regimes. breed differences were found in water intake and plasma prl concentrations when feed and water were ad lib., however, during any of the other 4 treatments, responses were similar between breeds. during dehydration and feed restriction, water intake, urine, fecal water, and plasma prolactin decr ... | 1985 | 2859961 |
| false negative result by the wellcozyme anti-hiv assay in testing an hiv positive haemophiliac. | | 1987 | 2883508 |
| [integrative nature of yersinia pestis virulence]. | | 1987 | 2883791 |
| polyester film as an advantageous tool for light and ultrastructural examination of single cells infected with viruses. | the use of marked transparent polyester film as a support of cell monolayer, smears or sections (cryostat or paraffin), makes possible to examine the same cell preselected in the light microscope by electron microscope (em). consequently, this technique enables to use histological or immunohistological investigations (using immunofluorescence or peroxidase (px) labelling) by light microscopy before examination in em. as example we used herpes simplex virus (hsv) type 1-infected cells grown on th ... | 1987 | 2883863 |
| a new subtype ("brezová") of tribec orbivirus (kemerovo group) isolated from ixodes ricinus males in czechoslovakia. | | 1987 | 2883865 |