| genetic analyses of the putative o and k antigen gene clusters of pandemic vibrio parahaemolyticus. | pandemic v. parahaemolyticus strains have rapidly changed their serotypes, but its determinants, especially k antigen, and the genes involved in serotype have been an open question. the purpose of this study was to gain insights into these points. although v. parahaemolyticus is known to be lacking o-side chain on its lipopolysaccharide, and o antigens are thought to be represented by core os, the genome sequence of v. parahaemolyticus o3:k6 strain rimd2210633 suggests that this bacterium potent ... | 2008 | 18557895 |
| proteomic analysis of exoproteins expressed by enterotoxigenic staphylococcus aureus strains. | pathogenic bacteria excrete a variety of virulence factors into extracellular medium and to the cell surface which have essential roles in the colonization and insurrection of the host cells, and thus reflect the degree of bacterial pathogenicity. for the exploration of virulence factors expressed in the secreted proteome fraction, different staphylococcus aureus strains were analyzed using gel-based bottom-up proteomic approach. a total of 119 distinct proteins were identified for the enterotox ... | 2008 | 18563740 |
| roles of cis- and trans-changes in the regulatory evolution of genes in the gluconeogenic pathway in yeast. | the yeast saccharomyces cerevisiae proliferates rapidly in glucose-containing media. as glucose is getting depleted, yeast cells enter the transition from fermentative to nonfermentative metabolism, known as the diauxic shift, which is associated with major changes in gene expression. to understand the expression evolution of genes involved in the diauxic shift and in nonfermentative metabolism within species, a laboratory strain (by), a wild strain (rm), and a clinical isolate (yjm) were used i ... | 2008 | 18573843 |
| breeding of d(-)-lactic acid high producing strain by low-energy ion implantation and preliminary analysis of related metabolism. | the low-energy nitrogen ion beam implantation technique was used in the breeding of mutant d(-)-lactic-acid-producing strains. the wild strain sporolactobacillus sp. dx12 was mutated by an n(+) ion beam with energy of 10kev and doses ranging from 0.4 x 10(15) to 6.60 x 10(15) ions/cm(2). combined with an efficient screening method, an efficient mutant y2-8 was selected after two times n(+) ion beam implantation. by using the mutant y2-8, 121.6g/l of d-lactic acid was produced with the molar yiel ... | 2010 | 18574566 |
| characterization of naturally occurring parainfluenza virus type 2 (hpiv-2) variants. | human parainfluenza viruses (hpiv) are respiratory pathogens responsible for upper and lower respiratory tract infections. in most labs, the clinical diagnosis of hpiv is routinely done using techniques based on the detection of viral antigens such as immunofluorescence assay or/and viral isolation. | 2008 | 18579437 |
| co-factor engineering in lactobacilli: effects of uncoupled atpase activity on metabolic fluxes in lactobacillus (l.) plantarum and l. sakei. | the hydrolytic f(1)-part of the f(1)f(0)-atpase was over-expressed in lactobacillus (l.) plantarum nc8 and l. sakei lb790x during fermentation of glucose or ribose, in order to study how changes in the intracellular levels of atp and adp affect the metabolic fluxes. the uncoupled atpase activity resulted in a decrease in intracellular energy level (atp/adp ratio), biomass yield and growth rate. interestingly, the glycolytic and ribolytic flux increased in l. plantarum with uncoupled atpase activ ... | 2008 | 18582592 |
| acute lymphadenitis with cellulitis caused by staphylococcus lugdunensis. | although coagulase-negative staphylococci (cns) have been considered part of the resident flora on the human skin, staphylococcus lugdunensis is an unusually virulent cns and can cause many types of infection. we report a rare case of acute lymphadenitis with cellulitis in the right infraauricular region caused by s. lugdunensis. a 62-yr-old woman visited the department of otolaryngology of busan paik university hospital. she had a palpable mass and swelling in the right infraauricular region an ... | 2008 | 18594171 |
| detection of a functional insertion sequence responsible for deletion of the thermostable direct hemolysin gene (tdh) in vibrio parahaemolyticus. | the thermostable direct hemolysin coded by the tdh gene is a marker of virulent strains of vibrio parahaemolyticus. the tdh genes are flanked by insertion sequences collectively named as isvs or their remnants; but the isvs so far examined have accumulated mutations in the transposase genes and underwent structural arrangements and their transposition activity could not be expected; the tdh gene was thus considered to have been acquired by v. parahaemolyticus through horizontal transfer in the p ... | 2008 | 18598741 |
| metabolic characterization of a l-lysine-producing strain by continuous culture. | continuous culture experiments with the l-producer, corynebacterium glutamicum, were carried out to characterize the effect of specific growth rate on fermentation yields, specific rates, productivities, and fluxes through the primary metabolism. the specific productivity of l-lysine exhibited a maximum with respect to specific growth rate, with an initial growth-associated behavior up to specific growth rates of about 0.1 h(-1), and a constant specific productivity for specific growth rates in ... | 1992 | 18600983 |
| effects of low, subinhibitory concentrations of antibiotics on expression of a virulence gene cluster of pathogenic escherichia coli by using a wild-type gene fusion. | s fimbrial adhesins (sfa) represent virulence factors of e. coli wild-type strains causing urinary tract infections and meningitis of the new born. in order to determine the influence of subinhibitory concentration of antibiotics on the expression of the sfa gene cluster, a wild-type strain carrying the lacz gene, coding for the enzyme beta-galactosidase fused to the sfa determinant was used. the expression of lacz which was under the control of the sfa wild-type promoters, was now equivalent to ... | 1993 | 18611540 |
| biological control against bacterial wilt and colonization of mulberry by an endophytic bacillus subtilis strain. | forty-five bacterial isolates were collected from surface-sterilized leaves of mulberry (morus alba l.). by screening their antagonistic activities against ralstonia solanacearum in vitro, four isolates showed a remarkable inhibitory effect. the evaluation of the antagonistic strains against bacterial wilt of mulberry indicated that the strain lu144 effectively reduced disease incidence. in the greenhouse, lu144 displayed effective biological control against bacterial wilt of mulberry when it wa ... | 2008 | 18631174 |
| acute nicotine reduces and repeated nicotine increases spontaneous activity in male and female lewis rats. | the lewis (lew) strain of rat appears more sensitive to nicotine than other strains in self-administration, conditioned place preference, and drug discrimination behavioral studies. the present study sought to further evaluate the behavioral effects of chronic nicotine treatment in the lew strain by assessing spontaneous activity, which has consistently revealed sensitization to chronic nicotine administration in sprague dawley (sd) rats. high active and low active male and female lew rats (n=8 ... | 2008 | 18644403 |
| [evaluation of newcastle disease virus with derivated hemagglutinin-neuraminidase gene of mesogenic strain]. | the hemagglutinin-neuraminidase (hn) and fusion protein of newcastle disease virus (ndv) plays a crucial role in the process of budding and infection. to understand the exact contribution of the hn gene to ndv pathogenecity, a reverse genetics system was developed using the lentogenic ndv lasota strain . | 2008 | 18652296 |
| [influence of cry2a sporulation-dependent promoter and molecular chaperone orf1-orf2 from bacillus thuringiensis on cry11aa protein]. | to analyze the coordination function of cry2a sporulation-dependent promoter and the enhanced expression of molecular chaperone orf1-orf2 to crystal protein cry11aa. | 2008 | 18652302 |
| enhancement of heterologous production of eicosapentaenoic acid in escherichia coli by substitution of promoter sequences within the biosynthesis gene cluster. | to enhance the heterologous production of eicosapentaenoic acid (epa) in escherichia coli, the epa biosynthesis gene cluster from shewanella oneidensis mr-1 was cloned under the lacz promoter on a high-copy number plasmid, pbluescript sk+. the production of epa was remarkably enhanced yielding levels of up to 7.5% of the total fatty acid content in the recombinant e. coli strain by induction with iptg, whereas the stimulation of epa production was abolished by adding glucose into the culture med ... | 2008 | 18661106 |
| [obtaining of the mutant yarrowia lipolytica strains producing citric acid from glucose]. | the possibility of obtaining mutant yeasts yarrowia lipolytica vkm y-2373 with increased ability to synthesize citric acid from glucose by using uv irradiation and n-methyl-nt-nitro-n-nitrosoguanidine was studied. of 1500 colonies of the y. lipolytica treated with either uv or n-methyl-nt-nitro-n-nitrosoguanidine, three mutants were selected that displayed higher (by 23%) biosynthetic ability as compared with the initial strain. additionally, three mutants were selected from 1000 colonies of the ... | 2008 | 18669266 |
| removal of antibiotic resistance gene-carrying plasmids from lactobacillus reuteri atcc 55730 and characterization of the resulting daughter strain, l. reuteri dsm 17938. | the spread of antibiotic resistance in pathogens is primarily a consequence of the indiscriminate use of antibiotics, but there is concern that food-borne lactic acid bacteria may act as reservoirs of antibiotic resistance genes when distributed in large doses to the gastrointestinal tract. lactobacillus reuteri atcc 55730 is a commercially available probiotic strain which has been found to harbor potentially transferable resistance genes. the aims of this study were to define the location and n ... | 2008 | 18689509 |
| in vitro antibacterial activity of psidium guajava linn. leaf extract on clinical isolates of multidrug resistant staphylococcus aureus. | in the present study, antibacterial activity of aqueous and organic extracts of psidium guajava leaves was evaluated against multidrug resistant (mdr) clinical isolates of staphylococcus aureus strains collected from hospitals in northern (malabar region) kerala. the strains which exhibited resistance against all the antibiotics tested was selected for antibacterial assays. minimum inhibitory concentration (mic) for methanolic and aqueous extracts was found to be 625 ug/ml and 7.5 mg/ml, respect ... | 2008 | 18697570 |
| in vivo manipulation of the bleomycin biosynthetic gene cluster in streptomyces verticillus atcc15003 revealing new insights into its biosynthetic pathway. | bleomycin (blm), an important clinically used antitumor compound, and its analogs are challenging to prepare by chemical synthesis. genetic engineering of the biosynthetic pathway in the producer strain would provide an efficient and convenient method of generating new derivatives of this complex molecule in vivo. however, the blm producing streptomyces verticillus atcc15003 has been refractory to all means of introducing plasmid dna into its cells for nearly two decades. several years after clo ... | 2008 | 18697737 |
| comparison of the membrane subproteomes during growth of a new pseudomonas strain on lysogeny broth medium, glucose, and phenol. | study of the bacterial membrane proteome is a field of growing interest in the research of nutrient transport and processing. pseudomonas sp. strain phdv1, a gram-negative bacterium selected for its ability to degrade aromatic compounds, was monitored under different growth substrate conditions, using lysogeny broth medium (lb), glucose, and phenol as sole carbon source. the aim of this study was to characterize the membrane subproteomes of the pseudomonas strain by proteomic means to assess the ... | 2008 | 18707154 |
| isolation of rifampicin resistant flavobacterium psychrophilum strains and their potential as live attenuated vaccine candidates. | previous studies have demonstrated that passage of pathogenic bacteria on increasing concentrations of the antibiotic rifampicin leads to the attenuation of virulence and these resistant strains may serve as live attenuated vaccines. two rifampicin resistant strains of flavobacterium psychrophilum, 259-93a.16 and 259-93b.17, were generated by passage on tyes plates containing increasing concentrations of rifampicin. electrophoretic analysis of whole-cell lysates prepared from the parent and resi ... | 2008 | 18708112 |
| [effects of different nuclear factor kappab dimers on the survival of immortalized neural progenitor cells]. | to investigate the effects of different nuclear factor (nf)-kb dimers on the survival of immortalized neural progenitor cells (inpcs). | 2008 | 18756949 |
| eimeria necatrix: selection and characteristics of a precocious (and attenuated) line. | a line of eimeria necatrix with an abbreviated life cycle (i.e. a "precocious" line) was derived from the houghton laboratory strain by repeated passage of the oocysts which were the first to be recovered from infections in chickens. the precocious line had a reproductive potential much lower than that of its parent strain and it was significantly less pathogenic. its immunogenicity was, however, substantially retained. selection for precocious development was accompanied by changes in the endog ... | 1984 | 18766877 |
| eimeria praecox: selection and characteristics of precocious lines. | precocious lines of eimeria praecox were obtained both from the houghton laboratory strain and the progeny of a single sporocyst (derived from the houghton strain) by selection for early development of oocysts in the mature host. the prepatent times of the parasites were reduced from 84 to less than 64 hours. the evolution of the trait for precocious development appeared to be a step-wise process culminating in the depletion of, probably, the fourth generation of schizont. studies on the precoci ... | 1984 | 18766878 |
| genetic analysis of fenhexamid-resistant field isolates of the phytopathogenic fungus botrytis cinerea. | the hydroxyanilide fenhexamid, one of the latest antibotrytis fungicides, active especially against leotiomycete plant-pathogenic fungi, inhibits 3-ketoreductase of the c-4-demethylation enzyme complex during ergosterol biosynthesis. we isolated botrytis cinerea strains resistant to various levels of fenhexamid from french and german vineyards. the sequence of the gene encoding 3-ketoreductase, erg27, varied according to levels of resistance. highly resistant isolates, termed hydr3(+), all prese ... | 2008 | 18779358 |
| diversity of sinorhizobium strains nodulating medicago sativa from different iranian regions. | alfalfa is believed to have originated in north-western iran and has a long history of coexistence with its bacterial symbiont sinorhizobium in soils of iran. however, little is known about the diversity of sinorhizobium strains nodulating iranian alfalfa genotypes. in this study, sinorhizobium populations were sampled from eight different iranian sites using three cultivars of medicago sativa as trap host plants. a total of 982 rhizobial strains were isolated and species were identified showing ... | 2008 | 18783438 |
| role of the histone-like nucleoid structuring protein in the regulation of rpos and rpos-dependent genes in vibrio cholerae. | production of the zn-metalloprotease hemagglutinin (ha)/protease by vibrio cholerae has been reported to enhance enterotoxicity in rabbit ileal loops and the reactogenicity of live cholera vaccine candidates. expression of ha/protease requires the alternate sigma factor sigma(s) (rpos), encoded by rpos. the histone-like nucleoid structuring protein (h-ns) has been shown to repress rpos expression in escherichia coli. in v. cholerae strains of the classical biotype, h-ns has been reported to sile ... | 2008 | 18790865 |
| intravenous tezosentan and vardenafil attenuate acute hypoxic pulmonary hypertension. | excessive hypoxic pulmonary hypertension imposes right ventricular strain by increasing afterload that may lead to right heart failure and death. increased phosphodiesterase activity, as well as increased levels of endothelin-1, has been discussed as molecular mechanisms. we investigated the hemodynamic and intrapulmonary effects of the intravenous dual endothelin a and b receptor blocker tezosentan, and of the phosphodiesterase-5 (pde-5) antagonist vardenafil in a pig model of acute normobaric ... | 2008 | 18800959 |
| distinct genomic replacements from lewis correct diastolic dysfunction, attenuate hypertension, and reduce left ventricular hypertrophy in dahl salt-sensitive rats. | hypertension and diastolic heart failure are two common cardiovascular diseases that inflict heavy morbidity and mortality, yet relatively little is understood about their pathophysiology. the identification of quantitative trait loci for blood pressure is important in unveiling the causes of polygenic hypertension. although dahl salt-sensitive strain is also an excellent model for the study of diastolic heart failure, virtually nothing is known about the quantitative trait loci determining dias ... | 2008 | 18806617 |
| identification of a nitroalkane oxidase gene: naoa related to the growth of streptomyces ansochromogenes. | naoa, encoding a nitroalkane oxidase that can catalyze toxic nitroalkanes to their corresponding aldehydes or ketones and hydrogen peroxide, was cloned from streptomyces ansochromogenes, but its function related to the growth of streptomyces is unknown. naoa was disrupted by the insertion of a kanamycin-resistance gene; the resulting strain can grow earlier than a wild-type strain under the same conditions. it was shown that naoa disruption accelerated growth of the naoa-disruption mutant, which ... | 2008 | 18810541 |
| [expression of green fluorescent protein (gfp) gene of aequorea victoria [correction of aequoria victoria] in lactobacillus plantarum bacterium ]. | results of development of shuttle expressing plasmid vector escherichia coli-lactobacillus which allowed high level expression of heterologous genes in lactobacilli are represented. vector ptrkh2 which is able to replicate in e. coli and in wide range of gram-positive bacteria was used as the base. in order to provide high level of cloned gene expression constitutive-active synthetic promoter, site of initiation of translation, and terminator of transcription were introduced in the vector. funct ... | 2008 | 18819405 |
| bioluminescent aspergillus fumigatus, a new tool for drug efficiency testing and in vivo monitoring of invasive aspergillosis. | aspergillus fumigatus is the main cause of invasive aspergillosis in immunocompromised patients, and only a limited number of drugs for treatment are available. a screening method for new antifungal compounds is urgently required, preferably an approach suitable for in vitro and in vivo studies. bioluminescence imaging is a powerful tool to study the temporal and spatial resolutions of the infection and the effectiveness of antifungal drugs. here, we describe the construction of a bioluminescent ... | 2008 | 18820063 |
| experimental transmission of field anaplasma marginale and the a. centrale vaccine strain by hyalomma excavatum, rhipicephalus sanguineus and rhipicephalus (boophilus) annulatus ticks. | the cattle rickettsia anaplasma marginale is distributed worldwide and is transmitted by about 20 tick species, but only rhipicephalus simus, a strictly african tick species, has been shown to transmit the vaccine strain of a. centrale. the aim of the present study was to examine transmission of field strains of a. marginale and of the vaccine strain of a. centrale by three tick species -hyalomma excavatum, rhipicephalus sanguineus and rhipicephalus (boophilus) annulatus - to susceptible calves. ... | 2009 | 18823724 |
| the potent non-competitive mglu1 receptor antagonist bay 36-7620 differentially affects synaptic plasticity in area cornu ammonis 1 of rat hippocampal slices and impairs acquisition in the water maze task in mice. | in this study we evaluated the effects of the novel, potent non-competitive metabotropic glutamate receptor (mglur) 1 antagonist (3as,6as)-6a-naphthalen-2-ylmethyl-5-methyliden-hexahydro-cyclopental[c]furan-1-on (bay 36-7620) on different types of synaptic plasticity in the hippocampal cornu ammonis (ca) 1-region and on hippocampus-dependent spatial learning. after having confirmed the presence of mglur1 in the hippocampal ca1 region of our rat strain by confocal microscopy, we tested the effect ... | 2008 | 18832015 |
| [identification of genes for biofilm formation in a salmonella enteritidis strain by transposon mutagenesis]. | biofilm plays an important role in pathogenicity of salmonella and food poisoning caused by salmonella. our aim was to identify genes associated with salmonella biofilm formation. | 2008 | 18837362 |
| a reusable method for construction of non-marker large fragment deletion yeast auxotroph strains: a practice in torulopsis glabrata. | a reusable method for construction of yeast auxotrophic mutants with large deletions that do not contain markers was developed and successfully applied to a pyruvate overproducing yeast strain, torulopsis glabrata cctcc m202019. a ura3 knockout fragment with a large deletion of the open reading frame and long homologous arms was constructed by fusion pcr and transformed into the parent strain by high-efficiency electroporation. a high concentration of transformed yeast was achieved by subsequent ... | 2009 | 18840479 |
| replacing escherichia coli nad-dependent glyceraldehyde 3-phosphate dehydrogenase (gapdh) with a nadp-dependent enzyme from clostridium acetobutylicum facilitates nadph dependent pathways. | reactions requiring reducing equivalents, nad(p)h, are of enormous importance for the synthesis of industrially valuable compounds such as carotenoids, polymers, antibiotics and chiral alcohols among others. the use of whole-cell biocatalysis can reduce process cost by acting as catalyst and cofactor regenerator at the same time; however, product yields might be limited by cofactor availability within the cell. thus, our study focussed on the genetic manipulation of a whole-cell system by modify ... | 2008 | 18852061 |
| the lack of rhodanese rhda affects the sensitivity of azotobacter vinelandii to oxidative events. | the rhda gene of azotobacter vinelandii codes for rhda, a rhodanese-domain protein with an active-site loop structure which has not currently been found in proteins of the rhodanese-homology superfamily. considering the lack of information on the functional role of the ubiquitous rhodaneses, in the present study we examined the in vivo functions of rhda by using an a. vinelandii mutant strain (mv474), in which the rhda gene was disrupted by deletion. preliminary phenotypic characterization of th ... | 2009 | 18925874 |
| glucose uptake and its effect on gene expression in prochlorococcus. | the marine cyanobacteria prochlorococcus have been considered photoautotrophic microorganisms, although the utilization of exogenous sugars has never been specifically addressed in them. we studied glucose uptake in different high irradiance- and low irradiance-adapted prochlorococcus strains, as well as the effect of glucose addition on the expression of several glucose-related genes. glucose uptake was measured by adding radiolabelled glucose to prochlorococcus cultures, followed by flow cytom ... | 2008 | 18941506 |
| [staphylococcus aureus colonization and risk of developing a subsequent peritonitis episode caused by an identical strain, among pediatric patients admitted for continuous ambulatory peritoneal dialysis]. | to determine the risk of pediatric end stage renal disease patients undergoing continuous ambulatory peritoneal dialysis to develop a subsecuent peritonitis episode caused by an identical staphylococcus aureus (sa) strain. | 2008 | 18942263 |
| mathematical models of cross protection in the epidemiology of plant-virus diseases. | abstract mathematical models of plant-virus disease epidemics were developed where cross protection occurs between viruses or virus strains. such cross protection can occur both naturally and through artificial intervention. examples of diseases with continuous and discontinuous crop-host availability were considered: citrus tristeza and barley yellow dwarf, respectively. analyses showed that, in a single host population without artificial intervention, the two categories of host plants, infecte ... | 2001 | 18944118 |
| aerobic fermentation of d-glucose by an evolved cytochrome oxidase-deficient escherichia coli strain. | fermentation of glucose to d-lactic acid under aerobic growth conditions by an evolved escherichia coli mutant deficient in three terminal oxidases is reported in this work. cytochrome oxidases (cydab, cyoabcd, and cbdab) were removed from the e. coli k12 mg1655 genome, resulting in the ecom3 (e. coli cytochrome oxidase mutant) strain. removal of cytochrome oxidases reduced the oxygen uptake rate of the knockout strain by nearly 85%. moreover, the knockout strain was initially incapable of growi ... | 2008 | 18952873 |
| introgression of a disrupted cadherin gene enables susceptible helicoverpa armigera to obtain resistance to bacillus thuringiensis toxin cry1ac. | a disrupted allele (r1) of a cadherin gene (ha_btr) is genetically associated with incompletely recessive resistance to bacillus thuringiensis toxin cry1ac in a cry1ac-selected strain (gybt) of helicoverpa armigera. the r1 allele of ha_btr was introgressed into a susceptible scd strain by crossing the gybt strain to the scd strain, followed by repeated backcrossing to the scd strain and molecular marker assisted family selection. the introgressed strain (designated as scd-r1, carrying homozygous ... | 2009 | 18954492 |
| [screening, breeding and metabolic analysis of a succinic-acid-producing strain]. | in order to obtain high yield mutant strains for the industrial bioconversion of succinic acid, we analyzed the metabolic networks of the strain actinobacillus succinogenes s.jst in the course of screening and breeding. | 2008 | 18956754 |
| [isolation, identification and characterization of nitroso-bacteria in one-step completely nitrogen removal process]. | to study the characteristics of the nitroso-bacteria in one-step completely autotrophic nitrogen removal process. | 2008 | 18956760 |
| virulence and antigenicity of the szp-gene deleted streptococcus equi ssp. zooepidemicus mutant in mice. | streptococcus equi ssp. zooepidemicus (sez) causes serious disease in pigs. m-like protein (szp) of sez is a virulence factor with opsonin function. to fabricate better vaccine against sez, we have generated a szp-knockout strain by homologous recombination. two fragments, upstream and downstream of the szp gene, were obtained by genome walking. the vector was then used to delete a 1090 bp segment of szp gene from a strain of sez (atcc35246). the mutant strain had 1000-fold decrease in ld50 as c ... | 2009 | 18983882 |
| detection of myocardial ischaemia by epicardial accelerometers in the pig. | we describe a novel technique for continuous real-time assessment of myocardial ischaemia using a three-axis accelerometer. | 2009 | 19022793 |
| echocardiographic speckle tracking radial strain imaging to assess ventricular dyssynchrony in a pacing model of resynchronization therapy. | speckle tracking imaging is a promising new echocardiographic method to assess left ventricular (lv) mechanical dyssynchrony. our aim was to assess a new speckle tracking regional strain algorithm by comparison with angle-corrected tissue doppler (td) in an animal model of left bundle branch block and cardiac resynchronization therapy. | 2008 | 19041581 |
| treponema zioleckii sp. nov., a novel fructan-utilizing species of rumen treponemes. | during studies on fructan degradation in the rumen, a treponema-like bacterium able to utilize timothy grass fructan, commercial inulin and sucrose as the sole carbon source was recovered from sheep rumen. at least two different fructanolytic enzymes were identified in cell-free extracts of the isolated bacterium. characterization of the strain by a polyphasic approach indicated that it can be regarded as a representative of a new bacterial species within the genus treponema. electron microscopy ... | 2008 | 19054106 |
| replacement of atcc 25944t, the current type strain of melittangium lichenicola, with atcc 25946. request for an opinion. | it is proposed that the type strain of melittangium lichenicola (thaxter 1892) mccurdy 1971 is atcc 25946 (=dsm 14877), which was originally designated as a reference strain by mccurdy, and not atcc 25944(t), as given in the approved lists. swarm appearance, myxospore morphology and 16s rrna gene sequence data suggest that atcc 25946 is a representative of the species melittangium lichenicola, while strain atcc 25944 is clearly a member of the myxococcus-corallococcus clade. a request for an opi ... | 2008 | 19060095 |
| blood pressure and heart rate qtl in mice of the b6/d2 lineage: sex differences and environmental influences. | a quantitative trait locus (qtl) approach was used to define the genetic architecture underlying variation in systolic blood pressure (sbp) and heart rate (hr), measured indirectly on seven occasions by the tail cuff procedure. the tests were conducted in 395 f(2) adult mice (197 males, 198 females) derived from a cross of the c57bl/6j (b6) and dba/2j (d2) strains and in 22 bxd recombinant-inbred (ri) strains. interval mapping of f(2) data for the first 5 days of measurement nominated one statis ... | 2009 | 19066325 |
| [optimization and primary application of one molecular typing method of salmonella, enterobacteia repetitive intergenic consensus sequences-based pcr in salmonella]. | to optimize the reaction conditions of enterobacteia repetitive intergenic consensus sequences-based pcr (eric-pcr), a molecular typing method of salmonella, study the genomic dna eric-pcr fingerprint maps of salmonella standard strains and epidemic isolates, and supply data for a system of salmonella epidemiology investigation and homology tracing. | 2008 | 19069668 |
| anaplasma marginale yucatan (mexico) strain. assessment of low virulence and potential use as a live vaccine. | anaplasma marginale yucatan strain was found to have low virulence in cattle. we studied the virulence of this isolate by experimental inoculation of 113 susceptible cattle at increasing doses, after which only one animal required treatment for clinical disease. subsequently, 104 cattle received a live vaccine of this strain by inoculation, which induced immunoprotection after heterologous challenged exposure with a different a. marginale isolate. in this study 14% of the immunized cattle requir ... | 2008 | 19120183 |
| evaluation of bcg vaccine and mycobacterium bovis culture filtrate proteins against bovine tuberculosis. | the objective of this study was to evaluate the degree of protection against tb of three immunogens in calves experimentally challenged with a pathogenic mycobacterium bovis strain by identification of lesions during necropsy. twenty-four calves free of tb were distributed into four groups: group 1 was inoculated with m. bovis cfpe, group 2 with cfpe/ifn-gamma, group 3 with m. bovis bcg, and group 4 remained as control. after 6 months all animals were challenged with an m. bovis field strain, an ... | 2008 | 19120235 |
| genetic diversity among norwegian vibrio parahaemolyticus. | to examine the variability among environmental vibrio parahaemolyticus (including trh+ isolates) from norway, and to compare these to clinical isolates and isolates from imported foods. | 2008 | 19120665 |
| hla class i molecules consistently present internal influenza epitopes. | cytotoxic t lymphocytes (ctl) limit influenza virus replication and prevent morbidity and mortality upon recognition of hla class i presented epitopes on the surface of virus infected cells, yet the number and origin of the viral epitopes that decorate the infected cell are unknown. to understand the presentation of influenza virus ligands by human mhc class i molecules, hla-b*0702-presented viral peptides were directly identified following influenza infection. after transfection with soluble cl ... | 2009 | 19122146 |
| double mutation of the pdc1 and adh1 genes improves lactate production in the yeast saccharomyces cerevisiae expressing the bovine lactate dehydrogenase gene. | expression of a heterologous l: -lactate dehydrogenase (l: -ldh) gene enables production of optically pure l: -lactate by yeast saccharomyces cerevisiae. however, the lactate yields with engineered yeasts are lower than those in the case of lactic acid bacteria because there is a strong tendency for ethanol to be competitively produced from pyruvate. to decrease the ethanol production and increase the lactate yield, inactivation of the genes that are involved in ethanol production from pyruvate ... | 2009 | 19122995 |
| complete genome analysis and virulence characteristics of the louisiana west nile virus strain lsu-ar01. | west nile virus (wnv) is a member of the flaviriridae family, which can cause significant morbidity and mortality in birds, horses, and humans. the wnv-lsu-ar01 strain was isolated from a dead blue jay in louisiana in 2001. phylogenetic analysis using 75 full wnv genomes revealed that the lsu-ar01 strain belongs to a distinct subclade among the north american strains. the lsu-ar01 strain differed from the ny-99 prototypic strain by 26 nucleotides causing six amino acid changes. an asparagine-to- ... | 2009 | 19130199 |
| molecular analysis of us10, s3, and us2 in duck enteritis virus. | a 4554-bp fragment was amplified from the dev c-kce vaccine strain by single oligonucleotide nested polymerase chain reaction with partially known sequences for the dev us1 and us10 genes. three open reading frames containing the genes encoding us10, s3, and us2 were predicted using the editseq program (dnastar). the s3 and us2 genes have the same transcription orientation but are oriented head-to-head with respect to us10. the promoters and polyadenylation signals were predicted. two poly a seq ... | 2009 | 19130202 |
| bacterial resistance modifying tetrasaccharide agents from ipomoea murucoides. | as part of an ongoing project to identify oligosaccharides which modulate bacterial multidrug resistance, the chcl(3)-soluble extract from flowers of a mexican arborescent morning glory, ipomoea murucoides, through preparative-scale recycling hplc, yielded five lipophilic tetrasaccharide inhibitors of staphylococcusaureus multidrug efflux pumps, murucoidins xii-xvi (1-5). the macrocyclic lactone-type structures for these linear hetero-tetraglycoside derivatives of jalapinolic acid were establish ... | 2009 | 19136125 |
| reengineering of a corynebacterium glutamicum l-arginine and l-citrulline producer. | toward the creation of a robust and efficient producer of l-arginine and l-citrulline (arginine/citrulline), we have performed reengineering of a corynebacterium glutamicum strain by using genetic information of three classical producers. sequence analysis of their arg operons identified three point mutations (argr123, argg92(up), and argg45) in one producer and one point mutation (argb26 or argb31) in each of the other two producers. reconstitution of the former three mutations or of each argb ... | 2009 | 19139237 |
| sar study of clubbed [1,2,4]-triazolyl with fluorobenzimidazoles as antimicrobial and antituberculosis agents. | in the present study, a series of novel 2-[4-(1h-[1,2,4]-triazol-1-yl)phenyl]-1-substituted-4,6-difluoro-1h-benzo[d]imidazole derivatives are synthesized by the alkylation of 2-[4-(1h-[1,2,4]-triazol-1-yl)phenyl]-4,6-difluoro-1h-benzo[d]imidazole with substituted alkyl and aryl halides. the compounds were evaluated for their preliminary in-vitro antibacterial activity against pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, and salmonella typhosa and then were screened for their ... | 2009 | 19150154 |
| further studies on vertical transmission and persistence of salmonella enterica serovar enteritidis phage type 4 in chickens. | one-week-old commercial layers were infected orally with 10(8) colony forming units of salmonella enterica serovar enteritidis phage type 4. no mortality was observed. the inoculated organism was isolated in decreasing viable numbers from a number of tissues, particularly the spleen, liver and caeca. organisms present in the spleen were primarily localized within macrophages. no salmonella enteritidis organisms were isolated between 10 and 24 weeks of age, when the experiment was terminated afte ... | 2001 | 19184915 |
| characterization of erysipelothrix rhusiopathiae isolates from poultry, pigs, emus, the poultry red mite and other animals. | erysipelothrix rhusiopathiae is the causative agent of erysipelas in mammals and birds, especially pigs and poultry. in order to investigate the suitability of different subtyping methods for genetic and phenotypic similarities among swedish isolates of the organism, 45 isolates from poultry (n=23), pigs (n=17), emus (n=2) and the poultry red mite dermanyssus gallinae (n=3) were investigated by serotyping, pulsed-field gel electrophoresis (pfge) and antimicrobial susceptibility testing. sequence ... | 2009 | 19193500 |
| [fungicidal activity of liranaftate against dermatophytes]. | the fungicidal activities of the thiocarbamate antifungal agent liranaftate were compared to those of luliconazole, amorolfine hydrochloride and ketoconazole against twelve stock strains of three species of dermatophytes. the mics of 0.001-0.009 microg/ml of luliconazole against trichophyton rubrum (n=6)were the lowest among the agents tested, but its mccs were considerably higher. consequently, the antifungal potency of luliconazole was considered fungistatic. in contrast to this, the mccs of 0 ... | 2009 | 19194054 |
| discovery of anti-varicella zoster virus activity of polyether antibiotic cp-44161. | in search for new anti-varicella zoster virus (vzv) compounds with new mechanism of action, we applied a dna hybridization assay (dot blot method) for screening. using this method, we screened microbial products and found the polyether compound cp-44161 from the culture broth of an actinomycete strain. cp-44161 was previously reported as an anticoccidal agent, but there has been no claim of its antiviral activities. cp-44161 showed strong anti-vzv activity against poka strain by plaque reduction ... | 2009 | 19198636 |
| two methods for attenuating toxoplasma gondii tachyzoites rh strain by using ethanol extract of curcuma longa. | the ethanol extract of curcuma longa proved to be an attenuated agent against toxoplasma gondii (rh strain) tachyzoites in the mice peritoneal fluid by two incubation methods. groups of female mice were injected by attenuated tachyzoites as 200 tachyzoites / ml, which treated with the ethanol extract of curcuma longa was previously treated. the result was observed after one week, as no tachyzoites were found in the peritoneal fluid of the experimental infected mice. | 2008 | 19209778 |
| a new tunisian strain of bacillus thuringiensis kurstaki having high insecticidal activity and delta-endotoxin yield. | blb1 is a new bacillus thuringiensis kurstaki strain, isolated from a tunisian soil sample. assay of toxicity of blb1 crystal proteins resulted in an lc50 of 70.32 ng of toxin per mg of flour against third instar ephestia kuehniella with confidence limits of (31.6-109.04 ng). this lc50 is less than that of the commercial strains hd1 used as a reference. the characterization of this strain by scanning transmission electron microscopy, analysis of its cry genes content by pcr-sequencing, and analy ... | 2009 | 19214476 |
| fabc of streptomyces lydicus involvement in the biosynthesis of streptolydigin. | streptolydigin, a secondary metabolite produced by streptomyces lydicus, is a potent inhibitor of bacterial rna polymerases. it has been suggested that streptolydigin biosynthesis is associated with polyketide synthase (pks) and nonribosomal peptide synthetase (nrps). thus, there is great interest in understanding the role of fatty acid biosynthesis in the biosynthesis of streptolydigin. in this paper, we cloned a type ii fatty acid synthase (fas ii) gene cluster of fabdhcf from the genome of s. ... | 2009 | 19214500 |
| tensile strain induces integrin beta1 and ilk expression higher and faster in 3d cultured rat skeletal myoblasts than in 2d cultures. | according to previous research, integrin beta1 and ilk play an important role in the extracellular matrix (ecm)-integrin-cytoskeleton pathway for mechanotransduction. the aim of this study was to investigate strain induced integrin beta1 and ilk expression in three-dimensional (3d) and in two-dimensional (2d) cultured rat skeletal myoblasts. sprague-dawley (sd) rat skeletal myoblasts were isolated and seeded on the plga-collagen composite scaffolds. the 3d cultured and the conventionally 2d mono ... | 2009 | 19215949 |
| screening and characterization of monoclonal antibodies to the surface antigens of listeria monocytogenes serotype 4b. | this study aims to develop and characterize monoclonal antibodies (mabs) with high specificity and affinity for surface antigens of an epidemiologically important serotype 4b of listeria monocytogenes. | 2009 | 19226395 |
| an improved protocol for electroporation of oenococcus oeni atcc baa-1163 using ethanol as immediate membrane fluidizing agent. | to finalize an effective and reproducible electroporation procedure to transform oenococcus oeni atcc baa-1163 strain. | 2008 | 19241529 |
| structural and thermodynamic analysis of a conformationally strained circular permutant of barnase. | circular permutation of a protein covalently links its original termini and creates new ends at another location. to maintain the stability of the permuted structure, the termini are typically bridged by a peptide long enough to span the original distance between them. here, we take the opposite approach and employ a very short linker to introduce conformational strain into a protein by forcing its termini together. we join the n- and c-termini of the small ribonuclease barnase (normally 27.2 a ... | 2009 | 19260676 |
| combinatorial gene overexpression and recessive mutant gene introduction in sake yeast. | industrial yeast strains are generally diploid and are often defective in sporulation. such strains are hence thought to be less tractable for manipulation by genetic engineering. to facilitate more reliable genetic manipulation of the diploid yeast japanese sake, we constructed variants of this strain that were homozygous for a ura3 deletion, homozygous for either mata or matalpha, and homozygous for either the his3 or the lys4 mutation. a ura3-null genotype enabled gene targeting to be underta ... | 2009 | 19270382 |
| [cloning of infectious bursal disease virus (gt strain) lack of vp5 gene]. | infectious bursal disease virus (ibdv) belongs to genus avibirnavirus of the family birnaviridae. the genome of ibdv consists of two segments of double-strand rna, which encode four structural protein vp1-vp4 and one non-structural protein vp5. | 2008 | 19271543 |
| in vitro tests to establish lc50 and discriminating concentrations for fipronil against rhipicephalus (boophilus) microplus (acari: ixodidae) and their standardization. | laboratory test was carried out on larvae and adults of the cattle tick, rhipicephalus (boophilus) microplus, to determine fipronil toxicity. adult immersion test (ait, n=26), larval immersion test (lit, n=71) and larval packet test (lpt, n=41) were standardized using susceptible strain (mozo). dose-response curves were compared with a fipronil resistant strain. four variables were analyzed from ait results: mortality, weight of eggs on day 7 and on day 14, index of fertility, and index of fecun ... | 2009 | 19278787 |
| [spontaneous and induced variability of carotenoid biosynthesis characteristics in streptomyces globisporus 1912]. | spontaneous and induced variability of carotenoid biosynthesis was studied in seven strains of streptomyces globisporus 1912 with the model of direct and reverse mutations. the appearance of spontaneous pigmentless (crt) colonies in mutants 4 crt and 7 crt was 0.9% at an average. crt(-) mutants were not observed among 6596 examined colonies of the strain 6 crt. the rvcrt strain has high level of carotenoid biosynthesis variability: the frequency of appearance of crt(-)-mutants was 22.1%. nitroso ... | 2008 | 19351044 |
| simultaneous cloning and expression of two cellulase genes from bacillus subtilis newly isolated from golden takin (budorcas taxicolor bedfordi). | a bacterial strain with high cellulase activity was isolated of feces sample of golden takin (budorcas taxicolor bedfordi). the bacterium was classified and designated bacillus subtilis ln by morphological and 16srdna gene sequence analysis. two putative cellulase genes, cell15 and cell73, were simultaneously cloned from the isolated strain by pcr. the putative gene cell15 consisted of an open reading frame (orf) of 1470 nucleotides and encoded a protein of 490 amino acids with a molecular weigh ... | 2009 | 19366617 |
| optimizing the selection process of yeast starter cultures by preselecting strains dominating spontaneous fermentations. | we propose an efficient and time-saving strategy for starter culture selection. our approach is based on the accomplishment of 3 phases: (i) the selection of yeast strains dominating spontaneous fermentations, (ii) the selection among the dominant strains of those showing the best technological characteristics, and (iii) the final selection among good technological strains of those showing the desired qualitative traits. we applied this approach to wine fermentations, even though the same strate ... | 2009 | 19370076 |
| antimicrobial activity of flavanoid sulphates and other fractions of argyreia speciosa (burm.f) boj. | antimicrobial activity of flavanoid sulphates and different fractions of a. speciosa root was studied against bacteria, fungi and mycobacterium tuberculosis h37 rv sensitive strain by in vitro and in vivo assays. flavanoid sulphates such as quercetin 3'7 di-o methyl 3- sulphate and kaempferol 7-o methyl 3-sulphate were isolated from the n-butanol fraction of 80% methanolic extract of the plant. the structures of the isolated flavanoids were confirmed by spectral studies. ethyl acetate (eaas) fra ... | 2009 | 19374167 |
| isolation, characterisation and kinetic studies on seva tb es-31 antigen, a metallo-serine protease of interest in serodiagnosis. | seva tb excretory secretory-31 (es-31) antigen, a glycoprotein isolated from m. tb h37ra culture filtrate, was found to be useful in the serodiagnosis of pulmonary tuberculosis (tb), extrapulmonary tb and in hiv-tb coinfection. further, it has been shown to be a zinc containing serine protease. | 2009 | 19402269 |
| caprine herpesvirus-1-specific igg subclasses in naturally and experimentally infected goats. | enzyme-linked immunosorbent assays (elisas) were developed to detect caprine herpervirus-1 (cphv-1)-specific igg1 and igg2 in sera from 43 naturally infected goats. the analysis of the igg subclasses showed a dual pattern of distribution in seropositive goats with a major group of animals (36 out of 43) exhibiting significantly higher levels of igg2 over igg1 and a minor group (7 out of 43) possessing equal levels of igg1 and igg2. four goats were experimentally infected with a virulent cphv-1 b ... | 2009 | 19411146 |
| hyperproduction of 3,4-dihydroxyphenyl-l-alanine (l-dopa) using erwinia herbicola cells carrying a mutant transcriptional regulator tyrr. | in the last few decades, enzymatic production of 3,4-dihydroxyphenyl-l-alanine (l-dopa) using tyrosine phenol-lyase (tpl) has been industrialized. this method has an intrinsic problem of tyrosine contamination because tpl is synthesized under tyrosine-induced conditions. herein, we constructed a hyper-l-dopa-producing strain by exploiting a mutant tyrr, an activator of tpl. the highest productivity was obtained for the strain grown under non-induced conditions. it was 30-fold higher than that ob ... | 2009 | 19420686 |
| design, synthesis and antimycobacterial activity of some novel imidazo[1,2-c]pyrimidines. | tuberculosis, due to its relentless nature, is now a major public health threat. the concomitant resurgence of tb with the mdr- or xdr-tb and hiv/aids pandemic has exposed the frailties of the current drug armatorium. based on isosteric replacement and good 3d structural similarity between pa-824, a novel antimycobacterial agent undergoing clinical trials, and imidazo[1,2-c]pyrimidines, we have designed novel imidazo[1,2-c]pyrimidines. the designed molecules were synthesized by nucleophilic disp ... | 2009 | 19423196 |
| expression of the tandem enhanced yellow fluorescent marker gene in toxoplasma gondii. | fluorescent protein is a useful tool for monitoring gene expression and studying biological processes of organisms including parasites. to improve the transfection efficiency and fluorescent intensity in toxoplasma gondii, a new transient expression vector, rges, containing the tandem enhanced yellow fluorescent protein gene, eyfp-eyfp, under the control of the parasite dense granule protein 1 (gra1) promoter was constructed. the rges plasmid was introduced into t. gondii rh strain by electropor ... | 2009 | 19424723 |
| determination of the regulon and identification of novel mrna targets of pseudomonas aeruginosa rsma. | one of the prokaryotic post-transcriptional regulatory mechanisms involves the csra/rsma family of proteins that act by modulating translation initiation at target mrnas. in this study, we identified the regulon of rsma of the pseudomonas aeruginosa pak strain by using cultures in the stationary phase of growth. the rsma regulon includes over 500 genes, of which approximately one-third were affected by an rsma mutation negatively, while the rest were affected positively. by isolating rsma/mrna c ... | 2009 | 19426209 |
| characterization of antigenic epitopes of the orf2 protein from hepatitis e virus genotype 4. | this study was aimed to characterize antigenic epitopes of a newly identified hepatitis e virus (hev) chinese strain of genotype 4. recombinant capsid protein p166chn (amino acids 464-629) and six murine monoclonal antibodies (mabs) against the protein were prepared. the mabs were evaluated for their ability to neutralize a genotype 4 hev strain by an in vitro pcr-based hev neutralization assay. the antigenic epitopes on hev genotype 4 and other different genotypes of hev strains were analyzed b ... | 2009 | 19428747 |
| genetic determination of the effect of post-translational modification on the innate immune response to the 19 kda lipoprotein of mycobacterium tuberculosis. | the 19 kda lipoprotein of mycobacterium tuberculosis (mtb) is an important target of the innate immune response. to investigate the effect of post-translation modification of this protein on innate recognition in the context of the whole bacillus, we derived a recombinant m. tuberculosis h37rv that lacked the 19 kda gene (delta19) and complemented this strain by reintroduction of the 19 kda gene into the chromosome as a single copy to produce delta19::19. we also reintroduced the 19 kda gene in ... | 2009 | 19442300 |
| hyperefficient prp sc amplification of mouse-adapted bse and scrapie strain by protein misfolding cyclic amplification technique. | abnormal forms of prion protein (prp(sc)) accumulate via structural conversion of normal prp (prp(c)) in the progression of transmissible spongiform encephalopathy. under cell-free conditions, the process can be efficiently replicated using in vitro prp(sc) amplification methods, including protein misfolding cyclic amplification. these methods enable ultrasensitive detection of prp(sc); however, there remain difficulties in utilizing them in practice. for example, to date, several rounds of prot ... | 2009 | 19459939 |
| generation and evaluation of an h9n1 influenza vaccine derived by reverse genetics that allows utilization of a diva strategy for control of h9n2 avian influenza. | h9n2 avian influenza viruses have circulated widely in domestic poultry around the world, and their outbreaks have resulted in heavy morbidity and mortality. in addition, h9n2 avian influenza viruses were transmitted directly from birds to humans in hong kong and mainland china during 1998 and 2003, which prompted the public health authorities to seek protective strategies to control h9n2 influenza viruses. in this study, we attempted to develop a diva (differentiating infected and vaccinated an ... | 2009 | 19543688 |
| synthesis of new 4-isopropylthiazole hydrazide analogs and some derived clubbed triazole, oxadiazole ring systems--a novel class of potential antibacterial, antifungal and antitubercular agents. | in the present study a series of 4-isopropylthiazole-2-carbohydrazide analogs, derived clubbed oxadiazole-thiazole and triazole-thiazole derivatives have been synthesized and characterized by ir, (1)h nmr, (13)c nmr, elemental and mass spectral analyses. the synthesized compounds were evaluated for their preliminary in vitro antibacterial, antifungal and antitubercular activity against mycobacterium tuberculosis h(37)rv strain by broth dilution assay method. the synthesized compounds 7a, 7b, 7d ... | 2009 | 19589626 |
| cd8+ cell noncytotoxic antiviral response in long-term hiv-1 infected former blood donors in china. | most of the hiv-infected long term survivors show strong cd8+ cell noncytotoxic antiviral response (cnar) that plays as an important factor for maintaining the relative healthy state of infected individuals. hiv infected former blood donors (fbds) in anhui, china are the unique population that considered infected by the same or a related hiv strain by the same exposure route, and is better to be studied for viral and host immunological factors associated with disease progression, such as cnar. w ... | 2009 | 19601780 |
| enhanced d-ribose biosynthesis in batch culture of a transketolase-deficient bacillus subtilis strain by citrate. | in this study, the effects of citrate addition on d-ribose production were investigated in batch culture of a transketolase-deficient strain, bacillus subtilis ec2, in shake flasks and bioreactors. batch cultures in shake flasks and a 5-l reactor indicated that supplementation with 0.2-0.5 g l(-1) of citrate enhanced d: -ribose production. when b. subtilis ec2 was cultivated in a 15-l reactor in a complex medium, the d: -ribose concentration was 70.9 g l(-1) with a ribose yield of 0.497 mol mol( ... | 2009 | 19603213 |
| evolutionary diversification indicated by compensatory base changes in its2 secondary structures in a complex fungal species, rhizoctonia solani. | the rrna cistron (18s-its1-5.8s-its2-28s) is used widely for phylogenetic analyses. recent studies show that compensatory base changes (cbc) in the secondary structure of its2 correlate with genetic incompatibility between organisms. rhizoctonia solani consists of genetically incompatible strain groups (anastomosis groups, ag) distinguished by lack of anastomosis between hyphae of strains. phylogenetic analysis of internal transcribed spacer (its) sequences shows a strong correlation with ag det ... | 2009 | 19609478 |
| xanthoradones, new potentiators of imipenem activity against methicillin-resistant staphylococcus aureus, produced by penicillium radicum fki-3765-2: i. taxonomy, fermentation, isolation and biological properties. | the fungal strain fki-3765-2, identified as penicillium radicum, was found to produce potentiators of imipenem activity against methicillin-resistant staphylococcus aureus (mrsa). two new compounds, designated xanthoradones a and b, were isolated from the fermentation broth of the producing strain by solvent extraction, octadecyl silyl column chromatography and preparative hplc. xanthoradones a and b potentiated imipenem activity against mrsa by decreasing the mic value of imipenem from 16 micro ... | 2009 | 19629141 |
| determination of mechanical properties of canine carpal ligaments. | to evaluate the mechanical properties of canine carpal ligaments for use in a finite element model of the canine antebrachium. | 2009 | 19645585 |
| viability of commercial wine yeasts during freezer storage in glycerol-based media. | glycerol-based medium (bm) with and without the addition of 1 g/l ascorbic acid (asc) and/or 100 mg/l (+/-)-catechin (cat) was tested for the storage of three commercial wine yeasts at -20 degrees c. the medium supplemented with asc was also used to store 706 strains to verify the maintenance of the liquid state. a decline in survival throughout the storage period was observed. the media containing asc maintained viability better than the other three. the bm caused a loss of viability of 7 order ... | 2009 | 19649740 |
| growth promotion and cell binding ability of bovine lactoferrin to bifidobacterium longum. | lactoferrin, a major whey protein of human milk, is considered as growth promoter for bifidobacteria, the predominant microorganisms of human intestine. in the present study, in vitro growth promotion and cell binding ability of bovine lactoferrin to several strains of bifidobacterium longum have been demonstrated. a dose-dependent as well as strain-dependent growth promotion effect by lactoferrin was observed. cell binding ability of lactoferrin was inspected under an inverted confocal laser sc ... | 2009 | 19655425 |
| intractable ulcer caused by mycobacterium shinshuense: successful identification of mycobacterium strain by 16s ribosomal rna 3'-end sequencing. | an extremely rare case of intractable ulcer caused by mycobacterium shinshuense is described. a 59-year-old japanese woman developed an ulcerated subcutaneous induration on the upper arm. ziehl-neelsen staining revealed positive bacilli. tissue culture isolated mycobacterium species, but standard identification techniques (including molecular biological approaches such as dna-dna hybridization) could not distinguish the precise causative pathogen, although it was narrowed down to three possibili ... | 2009 | 19663856 |
| lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells. | the study of a predicted outer membrane leptospiral protein encoded by the gene lic12690 in mediating the adhesion process. | 2009 | 19665803 |