| [increased apoptosis and down-regulation of rhoa in hepg2 cells infected by listeria monocytogenes]. | to explore the apoptosis of hepg2 cells infected by listeria monocytogenes egd strain (lm-egd) as well as rho family small gtpases rhoa expression. | 2016 | 27126939 |
| interrupting cd28 costimulation before antigen rechallenge affects cd8(+) t-cell expansion and effector functions during secondary response in mice. | the role of cd28-mediated costimulation in secondary cd8(+) t-cell responses remains controversial. here, we have used two tools - blocking mouse anti-mouse cd28-specific antibodies and inducible cd28-deleting mice - to obtain definitive answers in mice infected with ovalbumin-secreting listeria monocytogenes. we report that both blockade and global deletion of cd28 reveal its requirement for full clonal expansion and effector functions such as degranulation and ifn-γ production during the secon ... | 2016 | 27122236 |
| term-seq reveals abundant ribo-regulation of antibiotics resistance in bacteria. | riboswitches and attenuators are cis-regulatory rna elements, most of which control bacterial gene expression via metabolite-mediated, premature transcription termination. we developed an unbiased experimental approach for genome-wide discovery of such ribo-regulators in bacteria. we also devised an experimental platform that quantitatively measures the in vivo activity of all such regulators in parallel and enables rapid screening for ribo-regulators that respond to metabolites of choice. using ... | 2016 | 27120414 |
| restraining reactive oxygen species in listeria monocytogenes promotes the apoptosis of glial cells. | listeria monocytogenes is a facultative anaerobic foodborne pathogen that can traverse the blood-brain barrier and cause brain infection. l. monocytogenes infection induces host cell apoptosis in several cell types. in this study, we investigated the apoptosis of human glioma cell line u251 invaded by l. monocytogenes and evaluated the function of bacterial reactive oxygen species (ros) during infection. | 2016 | 27120397 |
| the continuous challenge of characterizing the foodborne pathogen listeria monocytogenes. | listeria monocytogenes is an important foodborne pathogen commonly isolated from food processing environments and food products. this organism can multiply at refrigeration temperatures, form biofilms on different materials and under various conditions, resist a range of environmental stresses, and contaminate food products by cross-contamination. l. monocytogenes is recognized as the causative agent of listeriosis, a serious disease that affects mainly individuals from high-risk groups, such as ... | 2016 | 27120361 |
| the microbiological quality of pasteurized milk sold by automatic vending machines. | the microbiological quality of pasteurized milk samples (n = 39) collected during 13 weekly intervals from three automatic vending machines (avm) in greece was investigated. microbiological counts (total aerobic (tac), total psychrotrophic (tpc), enterobacteriaceae (ec), and psychrotrophic aerobic bacterial spore counts (pabsc)) were obtained at the time of sampling and at the end of shelf-life (3 days) after storage of the samples at 4 or 8°c. tac were found to be below the 10(7 ) cfu ml(-1) li ... | 2016 | 27116619 |
| fully automated and colorimetric foodborne pathogen detection on an integrated centrifugal microfluidic device. | this work describes fully automated and colorimetric foodborne pathogen detection on an integrated centrifugal microfluidic device, which is called a lab-on-a-disc. all the processes for molecular diagnostics including dna extraction and purification, dna amplification and amplicon detection were integrated on a single disc. silica microbeads incorporated in the disc enabled extraction and purification of bacterial genomic dna from bacteria-contaminated milk samples. we targeted four kinds of fo ... | 2016 | 27112702 |
| click-em for imaging metabolically tagged nonprotein biomolecules. | em has long been the main technique for imaging cell structures with nanometer resolution but has lagged behind light microscopy in the crucial ability to make specific molecules stand out. here we introduce click-em, a labeling technique for correlative light microscopy and em imaging of nonprotein biomolecules. in this approach, metabolic labeling substrates containing bioorthogonal functional groups are provided to cells for incorporation into biopolymers by endogenous biosynthetic machinery. ... | 2016 | 27110681 |
| listeria monocytogenes infection differentially affects expression of ligands for nk cells and nk cell responses, depending on the cell type infected. | the pivotal role of nk cells in viral infection is extensively studied, whereas the role of nk cells in bacterial infection has been poorly investigated. here, we have examined how listeria monocytogenes (lm) affects expression of ligands for nk cell receptors and subsequent nk cell responses, depending on the type of cell infected. lm infected rat cell lines derived from different tissues were coincubated with splenic nk cells, and nk cell proliferation and ifn-γ production were measured. in ad ... | 2016 | 27106671 |
| listeriolysin o membrane damaging activity involves arc formation and lineaction -- implication for listeria monocytogenes escape from phagocytic vacuole. | listeriolysin-o (llo) plays a crucial role during infection by listeria monocytogenes. it enables escape of bacteria from phagocytic vacuole, which is the basis for its spread to other cells and tissues. it is not clear how llo acts at phagosomal membranes to allow bacterial escape. the mechanism of action of llo remains poorly understood, probably due to unavailability of suitable experimental tools that could monitor llo membrane disruptive activity in real time. here, we used high-speed atomi ... | 2016 | 27104344 |
| rifampin phosphotransferase is an unusual antibiotic resistance kinase. | rifampin (rif) phosphotransferase (rph) confers antibiotic resistance by conversion of rif and atp, to inactive phospho-rif, amp and pi. here we present the crystal structure of rph from listeria monocytogenes (rph-lm), which reveals that the enzyme is comprised of three domains: two substrate-binding domains (atp-grasp and rif-binding domains); and a smaller phosphate-carrying his swivel domain. using solution small-angle x-ray scattering and mutagenesis, we reveal a mechanism where the swivel ... | 2016 | 27103605 |
| in vivo efficacy of trans-cinnamaldehyde, carvacrol, and thymol in attenuating listeria monocytogenes infection in a galleria mellonella model. | listeria monocytogenes is a major foodborne pathogen that causes life-threatening illnesses in humans. with emergence of antibiotic resistance in l. monocytogenes, there is considerable interest in testing the efficacy of alternative therapies for controlling listeriosis in humans. this study investigated the efficacy of three phytochemicals, namely trans-cinnamaldehyde (tc), carvacrol (cr), and thymol (ty) in reducing l. monocytogenes virulence in the recently established invertebrate model, ga ... | 2016 | 27094514 |
| previous homologous and heterologous stress exposure induces tolerance development to pulsed light in listeria monocytogenes. | as one of the emerging non-thermal technologies, pulsed light (pl) facilitates rapid, mild and residue-free microbial surface decontamination of food and food contact materials. while notable progress has been made in the characterization of the inactivation potential of pl, experimental data available on the tolerance development to the same (homologous) stress or to different (heterologous) stresses commonly applied in food manufacturing (e.g., acid, heat, salt) is rather controversial. the fi ... | 2016 | 27092137 |
| implementation of nationwide real-time whole-genome sequencing to enhance listeriosis outbreak detection and investigation. | listeria monocytogenes (lm) causes severe foodborne illness (listeriosis). previous molecular subtyping methods, such as pulsed-field gel electrophoresis (pfge), were critical in detecting outbreaks that led to food safety improvements and declining incidence, but pfge provides limited genetic resolution. a multiagency collaboration began performing real-time, whole-genome sequencing (wgs) on all us lm isolates from patients, food, and the environment in september 2013, posting sequencing data i ... | 2016 | 27090985 |
| agricultural by-products with bioactive effects: a multivariate approach to evaluate microbial and physicochemical changes in a fresh pork sausage enriched with phenolic compounds from olive vegetation water. | the use of phenolic compounds derived from agricultural by-products could be considered as an eco-friendly strategy for food preservation. in this study a purified phenol extract from olive vegetation water (peovw) was explored as a potential bioactive ingredient for meat products using italian fresh sausage as food model. the research was developed in two steps: first, an in vitro delineation of the extract antimicrobial activities was performed, then, the peovw was tested in the food model to ... | 2016 | 27088870 |
| how many parameters does it take to describe disease tolerance? | the study of infectious disease has been aided by model organisms, which have helped to elucidate molecular mechanisms and contributed to the development of new treatments; however, the lack of a conceptual framework for unifying findings across models, combined with host variability, has impeded progress and translation. here, we fill this gap with a simple graphical and mathematical framework to study disease tolerance, the dose response curve relating health to microbe load; this approach hel ... | 2016 | 27088212 |
| a molluscan calreticulin ortholog from haliotis discus discus: molecular characterization and transcriptional evidence for its role in host immunity. | calreticulin (calr), a ca(2+) binding chaperone of the endoplasmic reticulum (er) and mainly involved in ca(2+) storage and signaling. in this study, we report the molecular characterization and immune responses of calr homolog from disk abalone (abcalr). the full length abcalr cdna (1837 bp) had an orf of 1224 bp. according to the multiple alignments analysis, n- and p-domains were highly conserved in all the selected members of calrs. in contrast, the c-domain which terminated with the charact ... | 2016 | 27086846 |
| how nacl and water content determine water activity during ripening of gouda cheese, and the predicted effect on inhibition of listeria monocytogenes. | this study describes the diffusion of nacl and water in gouda cheese during brining and ripening. furthermore, we established water activity as a function of the nacl-in-moisture content in gouda cheese during ripening. we determined nacl content, water content, and water activity in block-type gouda cheeses that were brined for 3.8d and foil-ripened for a period of 26 wk, and in wheel-type gouda cheeses that were brined for 0.33, 2.1, or 8.9d and subsequently nature-ripened for a period of 26 w ... | 2016 | 27085417 |
| listeria monocytogenes remodels the cell surface in the blood-stage. | after crossing the intestinal barrier, the bacterial pathogen listeria monocytogenes disseminates via the blood to the liver, spleen, brain and placenta. transcriptomic studies have shown that l. monocytogenes changes expression of many genes during this blood-stage. however, no comparable data at the protein level are known. as main interactors with the environment, we focused in surface proteins produced by l. monocytogenes in an ex vivo bovine blood model. bacteria exposed to blood alter sele ... | 2016 | 27085096 |
| simultaneous detection of waddlia chondrophila and listeria monocytogenes in aborted ruminant samples by real-time quantitative pcr. | | 2016 | 27083520 |
| international interlaboratory study comparing single organism 16s rrna gene sequencing data: beyond consensus sequence comparisons. | this study presents the results from an interlaboratory sequencing study for which we developed a novel high-resolution method for comparing data from different sequencing platforms for a multi-copy, paralogous gene. the combination of pcr amplification and 16s ribosomal rna gene (16s rrna) sequencing has revolutionized bacteriology by enabling rapid identification, frequently without the need for culture. to assess variability between laboratories in sequencing 16s rrna, six laboratories sequen ... | 2015 | 27077030 |
| proteolysis triggers self-assembly and unmasks innate immune function of a human α-defensin peptide. | human α-defensin 6 (hd6) is a unique peptide of the defensin family that provides innate immunity in the intestine by self-assembling to form high-order oligomers that entrap bacteria and prevent host cell invasion. here, we report critical steps in the self-assembly pathway of hd6. we demonstrate that hd6 is localized in secretory granules of small intestinal paneth cells. hd6 is stored in these granules as an 81-residue propeptide (prohd6), and is recovered from ileal lumen as a 32-residue mat ... | 2016 | 27076903 |
| validation of the applied biosystems 7500 fast instrument for detection of <em>listeria monocytogenes</em> with the suretect <em>listeria monocytogenes</em> pcr assay. | in 2013, the thermo scientific™ suretect™ listeria monocytogenes real-time pcr assay was certified by the aoac research institute (ri) performance tested methods (sm) program as a rapid method for the detection of l. monocytogenes from a wide range of food matrixes and surface samples. this report details the method modification studies undertaken to extend the analysis of this pcr assay to the applied biosystems™ 7500 fast pcr instrument and applied biosystems rapidfinder™ express 2.0 software ... | 2016 | 27075433 |
| il-12p40/il-10 producing precd8α/clec9a+ dendritic cells are induced in neonates upon listeria monocytogenes infection. | infection by listeria monocytogenes (lm) causes serious sepsis and meningitis leading to mortality in neonates. this work explored the ability of cd11c(high) lineage dcs to induce cd8+ t-cell immune protection against lm in mice before 7 days of life, a period symbolized by the absence of murine il-12p70-producing cd11c(high)cd8α+ dendritic cells (dcs). we characterized a dominant functional batf3-dependent precursor of cd11c(high) dcs that is clec9a+cd205+cd24+ but cd8α- at 3 days of life. afte ... | 2016 | 27074026 |
| picoliter well array chip-based digital recombinase polymerase amplification for absolute quantification of nucleic acids. | absolute, precise quantification methods expand the scope of nucleic acids research and have many practical applications. digital polymerase chain reaction (dpcr) is a powerful method for nucleic acid detection and absolute quantification. however, it requires thermal cycling and accurate temperature control, which are difficult in resource-limited conditions. accordingly, isothermal methods, such as recombinase polymerase amplification (rpa), are more attractive. we developed a picoliter well a ... | 2016 | 27074005 |
| inhibition of listeria monocytogenes in fresh cheese using chitosan-grafted lactic acid packaging. | a chitosan from biologically obtained chitin was successfully grafted with d,l-lactic acid (la) in aqueous media using p-toluenesulfonic acid as catalyst to obtain a non-toxic, biodegradable packaging material that was characterized using scanning electron microscopy, water vapor permeability, and relative humidity (rh) losses. additionally, the grafting in chitosan with la produced films with improved mechanical properties. this material successfully extended the shelf life of fresh cheese and ... | 2016 | 27070568 |
| matrix metalloproteinase-9 plays a role in protecting zebrafish from lethal infection with listeria monocytogenes by enhancing macrophage migration. | zebrafish could serve as an alternative animal model for pathogenic bacteria in multiple infectious routes. our previous study showed that immersion infection in zebrafish with listeria monocytogenes did not cause lethality but induced transient expression of several immune response genes. we used an affymetrix gene chip to examine the expression profiles of genes of zebrafish immersion-infected with l. monocytogenes. a total of 239 genes were up-regulated and 56 genes down-regulated compared wi ... | 2016 | 27068748 |
| the metalloprotease mpl supports listeria monocytogenes dissemination through resolution of membrane protrusions into vacuoles. | listeria monocytogenes is an intracellular pathogen that disseminates within the intestinal epithelium through acquisition of actin-based motility and formation of plasma membrane protrusions that project into adjacent cells. the resolution of membrane protrusions into vacuoles from which the pathogen escapes results in bacterial spread from cell to cell. this dissemination process relies on the mlp-acta-plcb operon, which encodes acta, a bacterial nucleation-promoting factor that mediates actin ... | 2016 | 27068088 |
| role of proapoptotic bh3-only proteins in listeria monocytogenes infection. | the ability of pathogens to influence host cell survival is a crucial virulence factor. listeria monocytogenes (lm) infection is known to be associated with severe apoptosis of hepatocytes and spleen cells. this impairs host defense mechanisms and thereby facilitates the spread of intracellular pathogens. the general mechanisms of apoptosis elicited by lm infection are understood, however, the roles of bh3-only proteins during primary lm infection have not been examined. to explore the roles of ... | 2016 | 27064265 |
| characterization of antimicrobial resistance of listeria monocytogenes strains isolated from a pork processing plant and its respective meat markets in southern china. | a total of 78 listeria monocytogenes isolates from a pork processing plant and the respective meat markets in southern china were examined. this number includes 60 isolates from pork at markets, 5 from cooked pork products at markets, 10 from pork at a processing plant, and 3 from food-contact surfaces at the processing plant. all isolates were subjected to serotyping, antimicrobial susceptibility testing, pulsed-field gel electrophoresis (pfge), and molecular basis of antibiotic resistance. fou ... | 2016 | 27058266 |
| genome sequences for a cluster of human isolates of listeria monocytogenes identified in south africa in 2015. | listeria monocytogenesis a gram-positive bacterium with a ubiquitous presence in the environment. there is growing concern about the increasing prevalence ofl. monocytogenesassociated with food-borne outbreaks. here we report genome sequences for a cluster of human isolates ofl. monocytogenesidentified in south africa in 2015. | 2016 | 27056221 |
| listeria meningitis in an immunocompetent child: a case report. | listeria monocytogenes is a facultative anerobic, gram-positive bacillus that is isolated from the soil, vegetables, and wild or domestic animals. listeria infection is usually found in the older adults, immunocompromised patients, pregnant women, and newborns, whereas it is rare in healthy infants and children. listeria monocytogenes may cause meningitis, meningoencephalitis, brain abscess, pyogenic arthritis, osteomyelitis, and liver abscess in children. the course of meningoencephalitis by li ... | 2016 | 27055168 |
| hygiene and safety in the meat processing environment from butcher shops: microbiological contamination and listeria monocytogenes. | the quality and safety of meat products can be estimated by assessing their contamination by hygiene indicator microorganisms and some foodborne pathogens, with listeria monocytogenes as a major concern. to identify the main sources of microbiological contamination in the processing environment of three butcher shops, surface samples were obtained from the hands of employees, tables, knives, inside butcher displays, grinders, and meat tenderizers (24 samples per point). all samples were subjecte ... | 2016 | 27052868 |
| viability of listeria monocytogenes on boneless, water-added hams, commercially prepared with and without food-grade chemicals, during extended storage at 4 and/or -2.2°c. | viability of listeria monocytogenes was monitored during refrigerated (4°c) and/or frozen (i.e., deep chilling at -2.2°c) storage on casing-cooked hams that were commercially prepared with and without potassium lactate and sodium diacetate (1.6%), buffered vinegar (2.2%), buffered vinegar and potassium lactate (1.7%), or a blend of potassium lactate, potassium acetate, and sodium diacetate (1.7%). a portion of these hams were subsequently surface treated with lauric arginate ester (lae; 44 ppm). ... | 2016 | 27052866 |
| effectiveness of broad-spectrum chemical produce sanitizers against foodborne pathogens as in vitro planktonic cells and on the surface of whole cantaloupes and watermelons. | over the past few years, foodborne disease outbreaks linked to enteric pathogens present on cantaloupe and watermelon surfaces have raised concerns in the melon industry. this research evaluated the effectiveness of commercially available produce sanitizers against selected foodborne pathogens, both in cell suspensions and on the outer rind surface of melons. the sanitizers (65 and 200 ppm of chlorine, 5 and 35% hydrogen peroxide, 5 and 50 ppm of liquid chlorine dioxide, various hydrogen peroxid ... | 2016 | 27052854 |
| efficacy of uv-c irradiation for inactivation of food-borne pathogens on sliced cheese packaged with different types and thicknesses of plastic films. | in this study, the efficacy of using uv-c light to inactivate sliced cheese inoculated with escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes and, packaged with 0.07 mm films of polyethylene terephthalate (pet), polyvinylchloride (pvc), polypropylene (pp), and polyethylene (pe) was investigated. the results show that compared with pet and pvc, pp and pe films showed significantly reduced levels of the three pathogens compared to inoculated but non-treated controls. the ... | 2016 | 27052716 |
| microbial and sensorial models for head-on and gutted (hog) atlantic salmon (salmo salar) stored from 0 to 15 °c. | predictive models offer efficient means to manage the quality and safety of highly perishable seafood. salmon is an increasingly popular seafood, and relies on well managed domestic and international supply chains to minimize growth of spoilage and pathogenic bacteria. while the literature describes predictive models for smoked and modified atmosphere packaged salmon, there are no reported models for spoilage bacteria and listeria monocytogenes on head-on and gutted (hog) aerobically-stored atla ... | 2016 | 27052713 |
| metagenomic analysis of a mexican ripened cheese reveals a unique complex microbiota. | cotija cheese is a mexican handcrafted product made from raw cow milk whose ripening process occurs spontaneously and, presumably, it is influenced by environmental conditions. its sensory characteristics and safety are probably the result of the balance between microbial populations and their metabolic capacity. in this work, we studied the dominance and richness of the bacteria in the cotija cheese microbiome, as well as their metabolic potential by high-throughput sequencing. by the analysis ... | 2016 | 27052710 |
| persistent and non-persistent strains of listeria monocytogenes: a focus on growth kinetics under different temperature, salt, and ph conditions and their sensitivity to sanitizers. | this study aimed to investigate the effect of different conditions, including temperature (37 °c, 22 °c, and 4 °c), nacl concentrations (2.5%, 4%, and 8%), and acidity (ph = 5), on the growth response of persistent and non-persistent isolates of listeria monocytogenes. the resistance to two common sanitizers (benzalkonium chloride and hydrogen peroxide) was also investigated. a selected group of 41 persistent and non-persistent l. monocytogenes isolates recovered from three cheese processing pla ... | 2016 | 27052708 |
| inhibition of listeria monocytogenes on cooked cured chicken breasts by acidified coating containing allyl isothiocyanate or deodorized oriental mustard extract. | ready-to-eat meats are considered foods at high risk to cause life-threatening listeria monocytogenes infections. this study screened 5 l. monocytogenes strains for their ability to hydrolyze sinigrin (a glucosinolate in oriental mustard), which formed allyl isothiocyanate (aitc) and reduced l. monocytogenes viability on inoculated vacuum-packed, cooked, cured roast chicken slices at 4 °c. tests involved incorporation of 25-50 μl/g aitc directly or 100-250 mg/g oriental mustard extract in 0.5% ( ... | 2016 | 27052706 |
| h3k4me3 demethylase kdm5a is required for nk cell activation by associating with p50 to suppress socs1. | the h3k4me3 demethylase kdm5a regulates gene transcription and is implicated in carcinogenesis. however, the role of kdm5a in innate immune response remains poorly understood. here, we demonstrate that kdm5a deficiency impairs activation of natural killer (nk) cells, with decreased ifn-γ production. accordingly, kdm5a(-/-) mice are highly susceptible to listeria monocytogenes (lm) infection. during nk cell activation, loss of kdm5a profoundly impairs phosphorylation and nuclear localization of s ... | 2016 | 27050510 |
| optimizing application parameters for lactic acid and sodium metasilicate against pathogens on fresh beef, pork and deli meats. | lactic acid (la) and sodium metasilicate (sm) have been approved for use as antimicrobials on meat. the objectives were to determine optimum concentrations, temperatures and hot-water dips of la and sm for reduction of escherichia coli o157:h7, non-o157 shiga-toxin producing e. coli (stec), salmonella spp., and listeria monocytogenes on beef, pork and deli meats. la was applied at 1, 2, 3, and 4% and sm was applied at 2, 3, 4, and 5%. sm4 and la4 were the lowest concentrations most effective aga ... | 2016 | 27050408 |
| nanoemulsions of thymol and eugenol co-emulsified by lauric arginate and lecithin. | lauric arginate (lae) is a cationic surfactant with excellent antimicrobial activities. to incorporate essential oil components (eocs) in aqueous systems, properties of eoc nanoemulsions prepared with a lae and lecithin mixture were studied. the lae-lecithin mixture resulted in stable translucent nanoemulsions of thymol and eugenol with spherical droplets smaller than 100nm, contrasting with the turbid emulsions prepared with individual emulsifiers. zeta-potential data suggested the formation of ... | 2016 | 27041312 |
| evidence of metabolically active but non-culturable listeria monocytogenes in long-term growth at 10 °c. | cultures of listeria monocytogenes at low temperatures (10 °c) in a broth model revealed long-term survival at about 0.04% cell density in relation to the log phase. in contrast, direct viable counts and pma real-time pcr data suggested that 50% and 1% of the population retain membrane integrity, respectively. to elucidate the observed difference, the metabolic activity of the bacterial population was investigated by multiparametric flow cytometry, including the assessment of membrane integrity, ... | 2016 | 27040843 |
| evaluation of a cross contamination model describing transfer of salmonella spp. and listeria monocytogenes during grinding of pork and beef. | in a previous study, a model was developed to describe the transfer and survival of salmonella during grinding of pork (møller, c.o.a., nauta, m.j., christensen, b.b., dalgaard, p., hansen, t.b., 2012. modelling transfer of salmonella typhimurium dt104 during simulation of grinding of pork. journal of applied microbiology 112 (1), 90-98). the robustness of this model is now evaluated by studying its performance for predicting the transfer and survival of salmonella spp. and listeria monocytogene ... | 2016 | 27035678 |
| genome sequences of five nonvirulent listeria monocytogenes serovar 4 strains. | we present the complete genome sequences of five nonpathogeniclisteria monocytogenesserovar 4 strains: wslc 1018 (4e), 1019 (4c), 1020 (4a), 1033 (4d), and 1047 (4d). these sequences may help to uncover genes involved in the synthesis of the serovar antigens-phenotypic determinants of virulence deemed clinically relevant. | 2016 | 27034489 |
| mechanistic studies of the agmatine deiminase from listeria monocytogenes. | listeria monocytogenes is a gram-positive food-borne pathogen that is capable of living within extreme environments (i.e. low temperatures and ph). this ability to survive in such conditions may arise, at least in part, from agmatine catabolism via the agmatine deiminase system (agds). this catabolic pathway utilizes an agmatine deiminase (agd) to hydrolyse agmatine into n-carbamoylputrescine (ncp), with concomitant release of ammonia, which increases the ph, thus mitigating the ill effects of t ... | 2016 | 27034081 |
| the regulatory roles of ncrna rli60 in adaptability of listeria monocytogenes to environmental stress and biofilm formation. | listeria monocytogenes is a facultative anaerobic gram-positive bacterium. it is well adapted to external environments and able to infect both humans and animals. to understand the impacts of ncrna rli60 on the adaptability of l. monocytogenes to environmental stresses and biofilm formation, a rli60 deletion strain of l. monocytogenes (lm-δrli60) was constructed using splicing by overlap extension pcr (soe-pcr) and homologous recombination and then compared it with wild-type strain l. monocytoge ... | 2016 | 27032404 |
| retail deli slicer cleaning frequency--six selected sites, united states, 2012. | listeria monocytogenes (listeria) causes the third highest number of foodborne illness deaths (an estimated 255) in the united states annually, after nontyphoidal salmonella species and toxoplasma gondii (1). deli meats are a major source of listeriosis illnesses, and meats sliced and packaged at retail delis are the major source of listeriosis illnesses attributed to deli meat (4). mechanical slicers pose cross-contamination risks in delis and are an important source of listeria cross-contamina ... | 2016 | 27031689 |
| effect of fermented soybean products intake on the overall immune safety and function in mice. | various functional activities have been reported for the fermented soybean products doenjang (dj) and cheonggukjang (cgj), although no systemic investigations of their immune functions have been conducted to date. we examined the effects of an experimental diet of dj, cgj, or a mixture of unfermented raw material for 4 weeks on overall immunity and immune safety in mice. no significant alterations were observed in peripheral or splenic immune cells among groups. enhanced splenic natural killer c ... | 2017 | 27030201 |
| whole-genome sequencing used to investigate a nationwide outbreak of listeriosis caused by ready-to-eat delicatessen meat, denmark, 2014. | listeriosis is a serious foodborne infection. outbreaks of listeriosis occur rarely, but have often proved difficult to solve. in june 2014, we detected and investigated a listeriosis outbreak in denmark using patient interviews and whole-genome sequencing (wgs). | 2016 | 27025820 |
| comparing listeriosis risks in at-risk populations using a user-friendly quantitative microbial risk assessment tool and epidemiological data. | although infection by the pathogenic bacterium listeria monocytogenes is relatively rare, consequences can be severe, with a high case-fatality rate in vulnerable populations. a quantitative, probabilistic risk assessment tool was developed to compare estimates of the number of invasive listeriosis cases in vulnerable canadian subpopulations given consumption of contaminated ready-to-eat delicatessen meats and hot dogs, under various user-defined scenarios. the model incorporates variability and ... | 2016 | 27019157 |
| overexpression of angiotensin-converting enzyme in myelomonocytic cells enhances the immune response. | angiotensin-converting enzyme (ace) converts angiotensin i to the vasoconstrictor angiotensin ii and thereby plays an important role in blood pressure control. however, ace is relatively non-specific in its substrate specificity and cleaves many other peptides. recent analysis of mice overexpressing ace in monocytes, macrophages, and other myelomonocytic cells shows that these animals have a marked increase in resistance to experimental melanoma and to infection by listeria monocytogenes or meth ... | 2016 | 27018193 |
| acidified nitrite inhibits proliferation of listeria monocytogenes - transcriptional analysis of a preservation method. | sodium nitrite (nano2) is added as a preservative during raw meat processing such as raw sausage production to inhibit growth of pathogenic bacteria. in the present study it was shown in challenge assays that the addition of sodium nitrite indeed inhibited growth and survival of listeria monocytogenes in short-ripened spreadable raw sausages. furthermore, in vitro growth analyses were performed, which took into account combinations of various parameters of the raw sausage ripening process like t ... | 2016 | 27017279 |
| antimicrobial properties of microemulsions formulated with essential oils, soybean oil, and tween 80. | it was previously found that blending soybean oil with cinnamon bark oil (cbo), eugenol or thyme oil, tween 80, and equal masses of water and propylene glycol could be used to prepare microemulsions. in the present study, the objective was to determine the antimicrobial activity of the microemulsions in tryptic soy broth (tsb) and 2% reduced fat milk. in tsb, the minimum inhibitory concentration (mic) of cbo solubilized in microemulsions was up to 625 ppm against cocktails of listeria monocytoge ... | 2016 | 27016636 |
| silver as antibacterial toward listeria monocytogenes. | listeria monocytogenes is a serious foodborne pathogen that can contaminate food during processing and can grow during food shelf-life. new types of safe and effective food contact materials embedding antimicrobial agents, like silver, can play an important role in the food industry. the present work aimed at evaluating the in vitro growth kinetics of different strains of l. monocytogenes in the presence of silver, both in its ionic and nano form. the antimicrobial effect was determined by assay ... | 2016 | 27014230 |
| acidic electrolyzed water as a novel transmitting medium for high hydrostatic pressure reduction of bacterial loads on shelled fresh shrimp. | acidic electrolyzed water (aew), a novel non-thermal sterilization technology, is widely used in the food industry. in this study, we firstly investigated the effect of aew as a new pressure transmitting medium for high hydrostatic pressure (aew-hhp) processing on microorganisms inactivation on shelled fresh shrimp. the optimal conditions of aew-hhp for vibrio parahaemolyticus inactivation on sterile shelled fresh shrimp were obtained using response surface methodology: nacl concentration to ele ... | 2016 | 27014228 |
| characterization of antimicrobial activity against listeria and cytotoxicity of native melittin and its mutant variants. | antimicrobial peptides (amps) are relatively short peptides that have the ability to penetrate the cell membrane, form pores leading to cell death. this study compares both antimicrobial activity and cytotoxicity of native melittin and its two mutants, namely, melittin i17k (gigavlkvlttglpalkswikrkrqq) with a higher charge and lower hydrophobicity and mutant g1i (iigavlkvlttglpaliswikrkrqq) of higher hydrophobicity. the antimicrobial activity against different strains of listeria was investigate ... | 2016 | 27011349 |
| a structural comparison of listeria monocytogenes protein chaperones prsa1 and prsa2 reveals molecular features required for virulence. | listeria monocytogenes is a gram-positive environmental bacterium that lives within soil but transitions into a pathogen upon contact with a mammalian host. the transition of l. monocytogenes from soil dweller to cytosolic pathogen is dependent upon secreted virulence factors that mediate cell invasion and intracellular growth. prsa1 and prsa2 are secreted bacterial lipoprotein chaperones that contribute to the folding of proteins translocated across the bacterial membrane; prsa2 is required for ... | 2016 | 27007641 |
| prevalence and contamination levels of listeria monocytogenes in ready-to-eat foods in tokyo, japan. | we surveyed prevalence and contamination levels of listeria monocytogenes in ready-to-eat foods between 2000 and 2012 in tokyo. l. monocytogenes was isolated from 52 (1.7%) out of 2,980 samples. comparing the prevalence in the study period, 2.2% were positive in the former period (2000-2005) and 1.2% in the latter (2006-2012). using the most probable number (mpn) technique, 32 samples were contaminated with fewer than 0.3 l. monocytogenes/g, 10 samples with 0.3-1.0/g and 4 samples with more than ... | 2016 | 27000951 |
| immunotherapy with a her2-targeting listeria induces her2-specific immunity and demonstrates potential therapeutic effects in a phase i trial in canine osteosarcoma. | recombinant listeria vaccines induce tumor-specific t-cell responses that eliminate established tumors and prevent metastatic disease in murine cancer models. we used dogs with her2/neu(+) appendicular osteosarcoma, a well-recognized spontaneous model for pediatric osteosarcoma, to determine whether a highly attenuated, recombinant listeria monocytogenes expressing a chimeric human her2/neu fusion protein (adxs31-164) could safely induce her2/neu-specific immunity and prevent metastatic disease. | 2016 | 26994144 |
| virulence gene-associated mutant bacterial colonies generate differentiating two-dimensional laser scatter fingerprints. | in this study, we investigated whether a laser scatterometer designated bardot (bacterial rapid detection using optical scattering technology) could be used to directly screen colonies of listeria monocytogenes, a model pathogen, with mutations in several known virulence genes, including the genes encoding listeria adhesion protein (lap; lap mutant), internalin a (δinla strain), and an accessory secretory protein (δseca2 strain). here we show that the scatter patterns of lap mutant, δinla, and δ ... | 2016 | 26994085 |
| occurrence and antimicrobial resistance patterns of listeria monocytogenes isolated from vegetables. | although the consumption of fresh and minimally processed vegetables is considered healthy, outbreaks related to the contamination of these products are frequently reported. among the food-borne pathogens that contaminate vegetables is listeria monocytogenes, a ubiquitous organism that exhibits the ability to survive and multiply at refrigerated temperatures. this study aimed to evaluate the occurrence of l. monocytogenes in vegetables as well as the antimicrobial resistance of isolates. the res ... | 2016 | 26991279 |
| opposing role of tumor necrosis factor receptor 1 signaling in t cell-mediated hepatitis and bacterial infection in mice. | death receptor (dr) ligands such as tumor necrosis factor (tnf) have been identified as fundamental mediators of liver damage both in mouse models and in humans. while the essential site of function of dr signaling is conceivably the hepatocyte, a systematic analysis is missing. using mice with conditional gene ablation, we analyzed the tissue-specific function of dr signaling in t cell-dependent (concanavalin a) and independent (lipopolysaccharide/galactosamine) hepatitis and in models of bacte ... | 2016 | 26991125 |
| attenuating listeria monocytogenes virulence by targeting the regulatory protein prfa. | the transcriptional activator prfa, a member of the crp/fnr family, controls the expression of some key virulence factors necessary for infection by the human bacterial pathogen listeria monocytogenes. phenotypic screening identified ring-fused 2-pyridone molecules that at low micromolar concentrations attenuate l. monocytogenes cellular uptake by reducing the expression of virulence genes. these inhibitors bind the transcriptional regulator prfa and decrease its affinity for the consensus dna-b ... | 2016 | 26991105 |
| a hot water extract of aralia cordata activates bone marrow-derived macrophages via a myeloid differentiation protein 88-dependent pathway and protects mice from bacterial infection. | in traditional asian medicine, aralia cordata (ac) is a known as a pain reliever and anti-inflammatory drug. although several of its biological activities have been reported, the immunomodulatory effects of a hot water extract of ac (hac) have not yet been described. the aim of this study was to investigate whether hac modulates the activation of macrophages, which play important roles in innate immune responses against microbial pathogens, and if so, to determine the molecular mechanisms by whi ... | 2016 | 26989992 |
| molecular typing and epidemiology of human listeriosis cases, denmark, 2002-2012. | denmark has a high incidence of invasive listeriosis (0.9 cases/100,000 population in 2012). we analyzed patient data, clinical outcome, and trends in pulsed-field gel electrophoresis (pfge) and multilocus sequence typing (mlst) of listeria monocytogenes strains isolated in denmark during 2002-2012. we performed 2-enzyme pfge and serotyping on 559 isolates and mlst on 92 isolates and identified some correlation between molecular type and clinical outcome and patient characteristics. we found 178 ... | 2016 | 26982714 |
| trends in us hospital stays for listeriosis in infants. | although listeriosis is rare in infants, it is common for young infants with suspected serious bacterial infection to be treated empirically with agents selected, in part, for their activity against listeria monocytogenes. our objectives were to describe the recent epidemiology of hospital discharges for listeriosis among infants in the united states and to precisely estimate the incidence of listeriosis according to infant age and meningitis status. | 2016 | 26980754 |
| a meta-analysis of the rates of listeria monocytogenes and enterococcus in febrile infants. | a change in the epidemiology of pathogens causing serious bacterial infection (sbi) has been noted since original recommendations were made for the empirical antibiotic choices for young infants with fever. | 2016 | 26980752 |
| lmoh7858_0369, a gene encoding a putative leucine-rich repeat-containing protein, is required for virulence of listeria monocytogenes. | listeria monocytogenes possesses the highest number of leucine-rich repeat (lrr)-containing proteins among all gram-positive bacteria; these lrr-containing molecules are known as the 'internalin' family. to understand the functions of largely uncharacterized lrr-containing molecules, we constructed seven deletion mutants in the l. monocytogenes h7858 strain targeting genes in this family and tested their virulence. among the seven mutants, the δlmoh7858_0369 strain and the δlmoh7858_2546 strain ... | 2016 | 26976852 |
| genome sequences of listeria monocytogenes strains responsible for cheese- and cooked ham product-associated swiss listeriosis outbreaks in 2005 and 2011. | the complete genome sequences of three listeria monocytogenes serotype 1/2a strains, lm 3136, lm 3163, and lm n1546, which were responsible for listeriosis outbreaks in 2005 and 2011 in switzerland, are presented here. | 2016 | 26966206 |
| genome analysis of listeria monocytogenes sequence type 8 strains persisting in salmon and poultry processing environments and comparison with related strains. | listeria monocytogenes is an important foodborne pathogen responsible for the disease listeriosis, and can be found throughout the environment, in many foods and in food processing facilities. the main cause of listeriosis is consumption of food contaminated from sources in food processing environments. persistence in food processing facilities has previously been shown for the l. monocytogenes sequence type (st) 8 subtype. in the current study, five st8 strains were subjected to whole-genome se ... | 2016 | 26953695 |
| whole genome sequence analysis using jspecies tool establishes clonal relationships between listeria monocytogenes strains from epidemiologically unrelated listeriosis outbreaks. | in an effort to build a comprehensive genomic approach to food safety challenges, the fda has implemented a whole genome sequencing effort, genometrakr, which involves the sequencing and analysis of genomes of foodborne pathogens. as a part of this effort, we routinely sequence whole genomes of listeria monocytogenes (lm) isolates associated with human listeriosis outbreaks, as well as those isolated through other sources. to rapidly establish genetic relatedness of these genomes, we evaluated t ... | 2016 | 26950338 |
| physical and antimicrobial properties of anise oil loaded nanoemulsions on the survival of foodborne pathogens. | the purpose of this research was to investigate antimicrobial effects of nano emulsions of anise oil (ao) on the survival of common food borne pathogens, listeria monocytogenes and escherichia coli o157:h7. series of emulsions containing different level of anise oil as potential antimicrobial delivery systems were prepared. antimicrobial activities of bulk anise oil and its emulsions (coarse and nano) was tested by the minimum inhibitory concentration and time kill assay. our results showed that ... | 2016 | 26948596 |
| listeria monocytogenes: an emerging food-borne pathogen and its public health implications. | listeria monocytogenes is considered one of the most important food-borne pathogens transmitted to humans via contaminated food. the aim of the present study was to demonstrate the importance of l. monocytogenes as a food-borne pathogen. | 2016 | 26927456 |
| [inhibitory effect of cabozantinib against listeria monocytogenes invasion in caco-2 cells in vitro]. | to investigate the effect of c-met inhibitor cabozantinib (xl-184) in inhibiting listeria monocytogenes (lm) from invading caco-2 cells to reduce the cell injury. | 2016 | 26922021 |
| locked nucleic acid probe-based real-time pcr for the diagnosis of listeria monocytogenes in ruminants. | because of its high fatality rate, listeriosis ranks among the most important infectious diseases worldwide. although ruminants are known as natural reservoirs for listeria monocytogenes and a possible source of human listeriosis, studies of the prevalence and risk factors associated with ruminant listeriosis are limited to some developed countries. therefore, this report describes the development of a real-time pcr targeting the hly gene for the absolute quantification of l. monocytogenes based ... | 2016 | 26921518 |
| microrna-23a curbs necrosis during early t cell activation by enforcing intracellular reactive oxygen species equilibrium. | upon antigen engagement, augmented cytosolic reactive oxygen species (ros) are needed to achieve optimal t cell receptor (tcr) signaling. however, uncontrolled ros production is a prominent cause of necrosis, which elicits hyper-inflammation and tissue damage. hence, it is critical to program activated t cells to achieve ros equilibrium. here, we determined that mir-23a is indispensable for effector cd4(+) t cell expansion, particularly by providing early protection from excessive necrosis. mech ... | 2016 | 26921109 |
| analysis of transcriptional signatures in response to listeria monocytogenes infection reveals temporal changes that result from type i interferon signaling. | analysis of the mouse transcriptional response to listeria monocytogenes infection reveals that a large set of genes are perturbed in both blood and tissue and that these transcriptional responses are enriched for pathways of the immune response. further we identified enrichment for both type i and type ii interferon (ifn) signaling molecules in the blood and tissues upon infection. since type i ifn signaling has been reported widely to impair bacterial clearance we examined gene expression from ... | 2016 | 26918359 |
| evaluation of reduction of fraser incubation by 24h in the en iso 11290-1 standard on detection and diversity of listeria species. | the en iso 11290-1 method for the isolation of listeria monocytogenes from food is carried out using a double enrichment in fraser broths. while the method is effective it is also quite long requiring 4-7 days to process a contaminated food, and may be adversely affected by inter-strain and/or inter-species competition in samples containing mixed listeria populations. currently, we have little information on the impact of competition on food testing under routine conditions. food samples (n=130) ... | 2016 | 26913375 |
| molecular docking based virtual screening of compounds for inhibiting sortase a in l.monocytogenes. | listeriosis is considered as an important public health issue. sortase a (srta) is an enzyme with catalytic role in l. monocytogenes that breaks the junction between threonine and glycine in the lpxtg motif (a key motif in internalin a (inla) that plays an important role in listeriosis). inactivation of srta was shown to inhibit anchoring of the invasion protein inia. this is in addition to inhibiting peptidoglycan-associated lpxtg proteins. therefore, it is of interest to inhibit stra using pot ... | 2015 | 26912950 |
| erratum for henke et al., listeria monocytogenes spreads within the brain by actin-based intra-axonal migration. | | 2016 | 26912561 |
| meningoencephalitis and listeria monocytogenes, toxoplasma gondii and brucella spp. coinfection in a dolphin in italy. | listeria monocytogenes, toxoplasma gondii and brucella spp. can infect a wide range of species, including humans. in cetaceans, meningoencephalitis has been associated with t. gondii and brucella spp. infection, whereas to our knowledge, l. monocytogenes infection has not previously been reported. meningoencephalitis and l. monocytogenes, t. gondii and brucella spp. were identified by means of both direct and indirect laboratory techniques in an adult female striped dolphin stenella coeruleoalba ... | 2016 | 26912047 |
| nlrp3 inflammasome activation contributes to listeria monocytogenes-induced animal pregnancy failure. | listeria monocytogenes (lm), a foodborne pathogen, can cause pregnancy failure in animals, especially in ruminants. recent studies have shown that lm activates inflammasomes to induce il-1β release in macrophages, however, whether the inflammasome activation regulates lm-induced pregnancy failure remains largely unknown. here we used mouse model to investigate the molecular mechanism by which lm-induced inflammsome activation contributes to lm-associated pregnancy failure | 2016 | 26911557 |
| cd8(+) t cells of listeria monocytogenes-infected mice recognize both linear and spliced proteasome products. | cd8(+) t cells responding to infection recognize pathogen-derived epitopes presented by mhc class-i molecules. while most of such epitopes are generated by proteasome-mediated antigen cleavage, analysis of tumor antigen processing has revealed that epitopes may also derive from proteasome-catalyzed peptide splicing (pcps). to determine whether pcps contributes to epitope processing during infection, we analyzed the fragments produced by purified proteasomes from a listeria monocytogenes polypept ... | 2016 | 26909514 |
| analysis of multilocus sequence typing and virulence characterization of listeria monocytogenes isolates from chinese retail ready-to-eat food. | eighty listeria monocytogenes isolates were obtained from chinese retail ready-to-eat (rte) food and were previously characterized with serotyping and antibiotic susceptibility tests. the aim of this study was to characterize the subtype and virulence potential of these l. monocytogenes isolates by multilocus sequence typing (mlst), virulence-associate genes, epidemic clones (ecs), and sequence analysis of the important virulence factor: internalin a (inla). the result of mlst revealed that thes ... | 2016 | 26909076 |
| biodiversity and hypervirulence of listeria monocytogenes. | the integration of large, well-sampled collections of bacterial isolates with genomics and experimental methods provides opportunities for 'top-down' discovery of the genetic basis of phenotypes of interest. in a new report, the authors apply this approach to investigate the heterogeneity in manifestations of disease caused by listeria monocytogenes and demonstrate that a previously uncharacterized cellobiose pts system is involved in central nervous system infection. | 2016 | 26906682 |
| listeria monocytogenes mutants defective in gallbladder replication represent safety-enhanced vaccine delivery platforms. | the gram positive intracellular pathogen listeria monocytogenes represents a promising vaccine or therapeutic dna delivery vector that has been successfully administered to humans in clinical trials. however in generating listeria mutants with therapeutic potential it is important to balance safety attenuation with efficacy. here we show that l. monocytogenes mutants with a reduced capacity for murine gallbladder replication are capable of stimulating t cell responses in mice and protecting vacc ... | 2016 | 26905522 |
| controlling listeria monocytogenes scott a on surfaces of fully cooked turkey deli product using organic acid-containing marinades as postlethality dips. | this study evaluated the efficacy of organic acids applied singly or in combination as postlethality dips to sliced uncured turkey deli loaves to inhibit the growth of listeria monocytogenes (lm) scott a. treatments consisted of sodium lactate (sl; 3.6%), potassium lactate (pl; 3.6%), sodium citrate (sc; 0.75%), a combination of sl and sodium diacetate (sda; 0.25%), and a combination of sl/pl/sda, alongside appropriate negative and positive controls. products were inoculated with 10(4)-10(5) cfu ... | 2015 | 26904656 |
| antimicrobial eugenol nanoemulsion prepared by gum arabic and lecithin and evaluation of drying technologies. | the purpose of present work was to develop eugenol oil nanoemulsions using gum arabic and lecithin as food grade natural emulsifiers, and study their antimicrobial activity. in addition, our study also evaluated different drying techniques (spray drying and freeze drying) on the morphology and redispersibility of nanoemulsion powders. the optimal fabrication method, physicochemical and structural characterization, stability, and antimicrobial activity were investigated. results showed that nanoe ... | 2016 | 26902894 |
| towards lag phase of microbial populations at growth-limiting conditions: the role of the variability in the growth limits of individual cells. | the water activity (aw) growth limits of unheated and heat stressed listeria monocytogenes individual cells were studied. the aw limits varied from 0.940 to 0.997 and 0.951 to 0.997 for unheated and heat stressed cells, respectively. due to the above variability a decrease in aw results in the presence of a non-growing fraction in the population leading to an additional pseudo-lag in population growth. in this case the total apparent lag of the population is the sum of the physiological lag of t ... | 2016 | 26900994 |
| genes involved in listeria monocytogenes biofilm formation at a simulated food processing plant temperature of 15 °c. | listeria monocytogenes is a pathogenic foodborne bacterium whose persistence in food processing environments is in part attributed to its biofilm formation. most biofilm studies have been carried out at 30-37 °c rather than at temperatures found in the food processing plants (i.e., 10-20 °c). the objective of the present study was to mine for novel genes that contribute to l. monocytogenes biofilm formation at 15 °c using the random insertional mutagenesis approach. a library of 11,024 l. monocy ... | 2016 | 26900648 |
| genotypic and antimicrobial characterization of pathogenic bacteria at different stages of cattle slaughtering in southern brazil. | meat can be contaminated in different stages of the slaughtering process and the identification of these stages is the starting point to implement adequate control measures. the objectives of this study were to assess the presence of pathogenic microorganisms in cattle carcasses, to identify the most important contamination points of the slaughtering process, and to evaluate the possible risk factors related to them in a cattle slaughterhouse. to this aim, 108 cattle carcasses were sampled at th ... | 2016 | 26896744 |
| type i ifn does not promote susceptibility to foodborne listeria monocytogenes. | type i ifn (ifn-α/β) is thought to enhance growth of the foodborne intracellular pathogen listeria monocytogenes by promoting mechanisms that dampen innate immunity to infection. however, the type i ifn response has been studied primarily using methods that bypass the stomach and, therefore, fail to replicate the natural course of l. monocytogenes infection. in this study, we compared i.v. and foodborne transmission of l. monocytogenes in mice lacking the common type i ifn receptor (ifnar1(-/-)) ... | 2016 | 26895837 |
| systems level analyses reveal multiple regulatory activities of cody controlling metabolism, motility and virulence in listeria monocytogenes. | bacteria sense and respond to many environmental cues, rewiring their regulatory network to facilitate adaptation to new conditions/niches. global transcription factors that co-regulate multiple pathways simultaneously are essential to this regulatory rewiring. cody is one such global regulator, controlling expression of both metabolic and virulence genes in gram-positive bacteria. branch chained amino acids (bcaas) serve as a ligand for cody and modulate its activity. classically, cody was cons ... | 2016 | 26895237 |
| prevalence of listeria monocytogenes in raw milk in kerman, iran. | listeria monocytogenes as one of the most important pathogen in public health concerns is transmitted through consumption of contaminated food. the pathogen has been considered as a potential source of contamination of raw milk and dairy products. this research was aimed to investigate prevalence of l. monocytogenes in raw milk in kerman region. in the summer of 2011, a total number of one hundred raw milk samples were collected from bulk tanks of some dairy farms and tested for iap and acta gen ... | 2015 | 26893812 |
| identification of proteins derived from listeria monocytogenes inducing human dendritic cell maturation. | dendritic cells (dcs) are potent antigen-presenting cells (apcs) that can promote antitumor immunity when pulsed with tumor antigens and then matured by stimulatory agents. despite apparent progress in dc-based cancer immunotherapy, some discrepancies were reported in generating potent dcs. listeria monocytogenes as an intracellular microorganism is able to effectively activate dcs through engaging pattern-recognition receptors (prrs). this study aimed to find the most potent components derived ... | 2016 | 26886282 |
| antibacterial activity of aquatic gliding bacteria. | the study aimed to screen and isolate strains of freshwater aquatic gliding bacteria, and to investigate their antibacterial activity against seven common pathogenic bacteria. submerged specimens were collected and isolated for aquatic gliding bacteria using four different isolation media (dw, ma, sap2, and vy/2). gliding bacteria identification was performed by 16s rrna gene sequencing and phylogenetic analysis. crude extracts were obtained by methanol extraction. antibacterial activity against ... | 2016 | 26885469 |
| probabilistic models to predict listeria monocytogenes growth at low concentrations of nano2 and nacl in frankfurters. | this study developed probabilistic models to describe listeria monocytogenes growth responses in meat products with low concentrations of nano2 and nacl. a five-strain mixture of l. monocytogenes was inoculated in nbye (nutrient broth plus 0.6% yeast extract) supplemented with nano2 (0-141 ppm) and nacl (0-1.75%). the inoculated samples were then stored under aerobic and anaerobic conditions at 4, 7, 10, 12, and 15℃ for up to 60 d. growth response data [growth (1) or no growth (0)] for each comb ... | 2015 | 26877642 |
| [clinical characteristics of 35 non-pregnant patients with listeria monocytogenes sepsis]. | to better understand the clinical characteristics and predisposing factors in non-pregnant patients with listeria monocytogenes (listeria) sepsis. | 2016 | 26875580 |