| whole genome-based population biology and epidemiological surveillance of listeria monocytogenes. | listeria monocytogenes (lm) is a major human foodborne pathogen. numerous lm outbreaks have been reported worldwide and associated with a high case fatality rate, reinforcing the need for strongly coordinated surveillance and outbreak control. we developed a universally applicable genome-wide strain genotyping approach and investigated the population diversity of lm using 1,696 isolates from diverse sources and geographical locations. we define, with unprecedented precision, the population struc ... | 2016 | 27723724 |
| a chitosan-coated liposome encapsulating antibacterial peptide, apep10: characterisation, triggered-release effects and antilisterial activity in thaw water of frozen chicken. | contamination of listeria monocytogenes in food and their processing environment is a focus of attention in the food industry. to achieve the controlled release of antibacterial agents to a food processing environment contaminated by l. monocytogenes, chitosan-stabilised liposomes encapsulating apep10 (glarclagtl), an antibacterial peptide derived from boiled-dried anchovies, were prepared by utilising listeria toxins to activate the peptide release. characteristics including the particle size, ... | 2016 | 27713959 |
| estrous cycle and gestational age-dependent expression of members of the interleukin-36 subfamily in a semi-allogeneic model of infected and non-infected murine pregnancy. | the il-36 subfamily is a recently described group of cytokines with pro-inflammatory behavior, comprising three agonists (α, β, and γ), its receptor (r), and one antagonist (ra). the expression and function of il-36 subfamily members in the estrous cycle in healthy and infected pregnancy has not been described. we evaluated mrna and protein expression of il-36 family members during the estrous cycle, implantation, fetal development, and post-labor periods in a model of allogenic pregnancy in mic ... | 2016 | 27713746 |
| a single capsule formulation of rhb-104 demonstrates higher anti-microbial growth potency for effective treatment of crohn's disease associated with mycobacterium avium subspecies paratuberculosis. | most recently we reported that rhb‑104 triple antibiotics combination in culture is bactericidal and should be effective for treatment of crohn's disease (cd)-associated with mycobacterium avium subspecies paratuberculosis (map) (alcedo et al. in gut pathog 14:32, 2016). the combination exhibited unique synergistic antimicrobial growth activity. the proprietary rhb-104 capsule formulation contains active ingredients (63.3 % clarithromycin (cla), 6.7 % clofazimine (clo) and 30 % rifabutin (rif)). ... | 2016 | 27708718 |
| rt-qpcr analysis of 15 genes encoding putative surface proteins involved in adherence of listeria monocytogenes. | l. monocytogenes adherence to food-associated abiotic surfaces and the development of biofilms as one of the underlying reasons for the contamination of ready-to-eat products is well known. the over-expression of internalins that improves adherence has been noted in cells growing as attached cells or at elevated incubation temperatures. however, the role of other internalin-independent surface proteins as adhesins has been uncharacterized to date. using two strains each of weakly- and strongly-a ... | 2016 | 27706070 |
| preparation and characterization of agar-based nanocomposite films reinforced with bimetallic (ag-cu) alloy nanoparticles. | agar-based active nanocomposite films were prepared by incorporating silver-copper (ag-cu) alloy nanoparticles (nps) (0.5-4wt%) into glycerol plasticized agar solution. thermo-mechanical, morphological, structural, and optical properties of the nanocomposite films were characterized by texture analyzer, differential scanning calorimetry (dsc), scanning electron microscope (sem), x-ray diffraction (xrd), fourier transforms infrared (ftir) spectroscopy, and surface color measurement. tensile stren ... | 2017 | 27702525 |
| molecular analysis of the role of osmolyte transporters opuca and betl in listeria monocytogenes after cold and freezing stress. | listeria monocytogenes is a food-borne pathogen of humans and other animals. the striking ability to survive several stresses usually used for food preservation makes l. monocytogenes one of the biggest concerns to the food industry. this ubiquity can be partly explained by the ability of the organism to grow and persist at very low temperatures, a consequence of its ability to accumulate cryoprotective compound called osmolytes. a quantitative rt-pcr assay was used to measure mrna transcript ac ... | 2017 | 27695911 |
| distinct surveillance pathway for immunopathology during acute infection via autophagy and sr-bi. | the mechanisms protecting from immunopathology during acute bacterial infections are incompletely known. we found that in response to apoptotic immune cells and live or dead listeria monocytogenes scavenger receptor bi (sr-bi), an anti-atherogenic lipid exchange mediator, activated internalization mechanisms with characteristics of macropinocytosis and, assisted by golgi fragmentation, initiated autophagic responses. this was supported by scavenger receptor-induced local increases in membrane ch ... | 2016 | 27694929 |
| listeria monocytogenes in stone fruits linked to a multistate outbreak: enumeration of cells and whole-genome sequencing. | in 2014, the identification of stone fruits contaminated with listeria monocytogenes led to the subsequent identification of a multistate outbreak. simultaneous detection and enumeration of l. monocytogenes were performed on 105 fruits, each weighing 127 to 145 g, collected from 7 contaminated lots. the results showed that 53.3% of the fruits yielded l. monocytogenes (lower limit of detection, 5 cfu/fruit), and the levels ranged from 5 to 2,850 cfu/fruit, with a geometric mean of 11.3 cfu/fruit ... | 2016 | 27694232 |
| widespread shortening of 3' untranslated regions and increased exon inclusion are evolutionarily conserved features of innate immune responses to infection. | the contribution of pre-mrna processing mechanisms to the regulation of immune responses remains poorly studied despite emerging examples of their role as regulators of immune defenses. we sought to investigate the role of mrna processing in the cellular responses of human macrophages to live bacterial infections. here, we used mrna sequencing to quantify gene expression and isoform abundances in primary macrophages from 60 individuals, before and after infection with listeria monocytogenes and ... | 2016 | 27690314 |
| a look inside the listeria monocytogenes biofilms extracellular matrix. | listeria monocytogenes is a foodborne pathogen able to persist in food industry and is responsible for a severe illness called listeriosis. the ability of l. monocytogenes to persist in environments is due to its capacity to form biofilms that are a sessile community of microorganisms embedded in a self-produced matrix of extracellular polymeric substances (eps's). in this review, we summarized recent efforts performed in order to better characterize the polymeric substances that compose the ext ... | 2016 | 27681916 |
| phage inactivation of listeria monocytogenes on san daniele dry-cured ham and elimination of biofilms from equipment and working environments. | the anti-listerial activity of generally recognized as safe (gras) bacteriophage listex p100 (phage p100) was demonstrated in broths and on the surface of slices of dry-cured ham against 5 strains or serotypes (i.e., scott a, 1/2a, 1/2b, and 4b) of listeria monocytogenes. in a broth model system, phage p100 at a concentration equal to or greater than 7 log pfu/ml completely inhibited 2 log cfu/cm² or 3 log cfu/cm² of l. monocytogenes growth at 30 °c. the temperature (4, 10, 20 °c) seemed to infl ... | 2016 | 27681898 |
| anti-listerial activity of four seaweed essential oils against listeria monocytogenes. | listeria monocytogenes is one of the most virulent types of bacteria and causes severe foodborne illness, such as listeriosis. because this pathogen has become resistant to sanitizers and other disinfectants that are used to clean utensils and surfaces during food processing, it poses a serious threat to the food industry. | 2016 | 27679700 |
| uptake and metabolism of antibiotics roseoflavin and 8-demethyl-8-aminoriboflavin in riboflavin-auxotrophic listeria monocytogenes. | the riboflavin analogs roseoflavin (rof) and 8-demethyl-8-aminoriboflavin (af) are produced by the bacteria streptomyces davawensis and streptomyces cinnabarinus riboflavin analogs have the potential to be used as broad-spectrum antibiotics, and we therefore studied the metabolism of riboflavin (vitamin b2), rof, and af in the human pathogen listeria monocytogenes, a bacterium which is a riboflavin auxotroph. we show that the l. monocytogenes protein lmo1945 is responsible for the uptake of ribo ... | 2016 | 27672192 |
| a multiplex pcr for detection of listeria monocytogenes and its lineages. | a novel multiplex pcr assay was developed to identify genus listeria, and discriminate listeria monocytogenes and its major lineages (li, lii, liii). this assay is a rapid and inexpensive subtyping method for screening and characterization of l. monocytogenes. | 2016 | 27671346 |
| mitogen-activated protein kinases are required for effective infection of human choroid plexus epithelial cells by listeria monocytogenes. | listeria monocytogenes, a gram-positive bacterium, can cause meningitis after invading the human central nervous system. the blood-cerebrospinal fluid barrier (bcsfb), located at the epithelium of the choroid plexus, is a possible entry site for l. monocytogenes into the brain, and in vitro l. monocytogenes invades human choroid plexus epithelial papilloma (hibcpp) cells. although host cell signal transduction subsequent to infection by l. monocytogenes has been investigated, the role of mitogen ... | 2017 | 27671041 |
| effect of vitamin a on listeria monocytogenes infection in a silkworm model. | insect infection models have been used increasingly to study various pathogenic agents in evaluations of pathogenicity and drug efficacy. in this study, we demonstrated that larvae of the silkworm bombyx mori are useful for studying listeria monocytogenes infections in insects. infection with the l. monocytogenes wild-type strain induced silkworm death. infection by a listeriolysin o (llo) deletion mutant also induced silkworm death, but the bacterial numbers in silkworms were lower than those o ... | 2017 | 27669511 |
| impact of starch-based emulsions on the antibacterial efficacies of nisin and thymol in cantaloupe juice. | the use of antimicrobial compounds to prevent foodborne pathogens from contaminating fresh-cut produce has received broad attentions; however, the applications of these compounds are hindered by their rapid depletion in foods. to prolong their efficacies, the use of delivery systems is essential. in this study, oil-in-water emulsions formed using starch octenyl succinate (starch-os) were used to stabilize nisin and thymol in cantaloupe juice-containing fluid. listeria monocytogenes v7 and salmon ... | 2017 | 27664621 |
| insights into the mechanism of homeoviscous adaptation to low temperature in branched-chain fatty acid-containing bacteria through modeling fabh kinetics from the foodborne pathogen listeria monocytogenes. | the psychrotolerant foodborne pathogen listeria monocytogenes withstands the stress of low temperatures and can proliferate in refrigerated food. bacteria adapt to growth at low temperatures by increasing the production of fatty acids that increase membrane fluidity. the mechanism of homeoviscous increases in unsaturated fatty acid amounts in bacteria that predominantly contain straight-chain fatty acids is relatively well understood. by contrast the analogous mechanism in branched-chain fatty a ... | 2016 | 27656167 |
| human γδ t cells augment antigen presentation in listeria monocytogenes infection. | circulating γδ t cells in healthy individuals rapidly respond to bacterial and viral pathogens. many studies have demonstrated that γδ t cells are activated and expanded by listeria monocytogenes (l.monocytogenes), a foodborne bacterial pathogen with high fatality rates. however, the roles of γδ t cells during l.monocytogenes infection are not clear. in the present study, we characterized the morphological characteristics of phagocytosis in γδ t cells after l.monocytogenes infection using transm ... | 2016 | 27652377 |
| listeria rhombencephalitis complicating anti-tnf treatment during an acute flare of crohn's colitis. | patients with crohn's disease often require the use of immunosuppressant drugs to control disease activity. such medication includes steroids, azathioprine, and biologic therapy. these suppress the immune response, and the patient is more susceptible to infection. we present a case of a 69-year-old gentleman with a history of crohn's colitis who had ongoing symptoms of diarrhoea in spite of standard treatment. biologic therapy was considered to be the next step, and screening for infection was u ... | 2016 | 27651962 |
| rab32 restriction of intracellular bacterial pathogens. | our immune system is engaged in a continuous battle against invading pathogens, many of which have evolved to survive in intracellular niches of mammalian hosts. a variety of cellular processes are involved in preventing bacterial invasion or in killing bacteria that successfully invade host cells. recently, the rab gtpase rab32 emerged as critical regulator of a host defense pathway that can eliminate bacterial pathogens. salmonella enterica is an intracellular bacterium and a major cause of in ... | 2016 | 27645564 |
| tumor suppressor p53 protects mice against listeria monocytogenes infection. | tumor suppressor p53 is involved in regulating immune responses, which contribute to antitumor and antiviral activity. however, whether p53 has anti-bacterial functions remains unclear. listeria monocytogenes (lm) causes listeriosis in humans and animals, and it is a powerful model for studying innate and adaptive immunity. in the present study, we illustrate an important regulatory role of p53 during lm infection. p53 knockout (p53ko) mice were more susceptible to lm infection, which was manife ... | 2016 | 27644341 |
| listeria monocytogenes strains which are underrepresented during selective enrichment with the iso method might dominate during passage through simulated gastric fluid and in vitro infection of caco-2 cells. | various listeria monocytogenes strains may contaminate a single food potentially resulting in simultaneous exposure of consumers to multiple strains. however, l. monocytogenes strains isolated from foods by selective enrichment (se) might not always represent those that can better survive a patient immune system, due to bias in strain recovery. we investigated the effect of co-cultivation in tsb-y at 10°c for 8 days on i) the detection of l. monocytogenes strains during se with the iso 11290-1:1 ... | 2016 | 27637880 |
| genome sequence of the listeria monocytogenes food isolate hpb913, collected in canada in 1993. | listeria monocytogenes is a pathogenic bacterium of importance to public health and food safety agencies. we present the genome sequence of the serotype 1/2a l. monocytogenes food isolate hpb913, which was collected in canada in 1993 as part of an investigation into a sporadic case of foodborne illness. | 2016 | 27634991 |
| anti-listerial effect of selected essential oils and thymol. | the anti-listerial effect of marjoram, thyme essential oils (eos) and thymol on listeria monocytogenes inoculated chicken breast fillets was investigated. before inoculation the fillets were pretreated by washing or not under running tap water. inoculated samples were kept at 6 °c for 24 h to allow the growth of l. monocytogenes. after this, the fillets were put in marinating solutions containing salt (5%) and eos or thymol in mic/2 concentration established in vitro. total germ count (tgc) and ... | 2016 | 27630055 |
| intestinal organoids as a novel tool to study microbes-epithelium interactions. | the gut, particularly the colon, is the host of approximately 1000 bacterial species, the so-called gut microbiota. the relationship between the gut microbiota and the host is symbiotic and mutualistic, influencing many aspects of the biology of the host. this homeostatic balance can be disrupted by enteric pathogens, such as shigella flexneri or listeria monocytogenes, which are able to invade the epithelial layer and consequently subvert physiological functions. to study the host-microbe inter ... | 2016 | 27628134 |
| the correlation between nacl adaptation and heat sensitivity of listeria monocytogenes, a foodborne pathogen through fresh and processed meat. | this study examined the relationship between nacl sensitivity and stress response of listeria monocytogenes. nine strains of l. monocytogenes (nccp10805, nccp10806, nccp10807, nccp10808, nccp10809, nccp10810, nccp10811, nccp10920 and nccp 10943) were exposed to 0%, 1%, 2% and 4% nacl, and then incubated at 60℃ for 60 min to select strains that were heat-sensitized (hs) and non-sensitized (ns) by nacl exposure. after heat challenge, l. monocytogenes strains were categorized as hs (nccp 10805, ncc ... | 2016 | 27621687 |
| selective photocatalytic disinfection by coupling strepminisog to the antibody catalyzed water oxidation pathway. | for several decades reactive oxygen species have been applied to water quality engineering and efficient disinfection strategies; however, these methods are limited by disinfection byproduct and catalyst-derived toxicity concerns which could be improved by selectively targeting contaminants of interest. here we present a targeted photocatalytic system based on the fusion protein strepminisog that uses light within the visible spectrum to produce reactive oxygen species at a greater efficiency th ... | 2016 | 27617441 |
| bacterial meningitis in patients using immunosuppressive medication: a population-based prospective nationwide study. | we studied occurrence, presentation, disease course, effect of adjunctive dexamethasone, and prognosis of bacterial meningitis in patients using immunosuppressive medication. patients were selected from our nationwide, prospective cohort on community-acquired bacterial meningitis performed from march 1, 2006 through october 31, 2014. eighty-seven of 1447 episodes (6 %) of bacterial meningitis occurred in patients using immunosuppressive medication, and consisted of corticosteroids in 82 %. patie ... | 2016 | 27613024 |
| combination of cymbopogon citratus and allium cepa essential oils increased antibacterial activity in leafy vegetables. | cymbopogon citratus and allium cepa essential oils (eos) are rich in terpenes and sulfur compounds respectively, both with antibacterial activity and different cell targets, supporting the idea that their combination can increase their efficacy. | 2017 | 27611358 |
| draft genome sequences of 15 isolates of listeria monocytogenes serotype 1/2a, subgroup st204. | listeria monocytogenes sequence type 204 (st204) strains have been isolated from a range of food, environmental, and clinical sources in australia. this study describes the draft genome sequences of 15 isolates collected from meat and dairy associated sources. | 2016 | 27609916 |
| [bacterial meningitis in adults in emergency and rescue services]. | the cardinal symptoms of bacterial meningitis are headache, fever, impaired consciousness and nuchal stiffness (meningism); however, the diagnosis of acute bacterial meningitis can only be confirmed or ruled out by investigation of cerebrospinal fluid. the recommended empirical antibiotic regimen for community-acquired acute bacterial meningitis in adults in germany is a combination of ceftriaxone and ampicillin plus adjuvant dexamethasone. an important influenceable factor for treatment success ... | 2016 | 27600937 |
| extracellular superoxide dismutase enhances recruitment of immature neutrophils to the liver. | listeria monocytogenes is a gram-positive intracellular pathogen that causes spontaneous abortion in pregnant women, as well as septicemia, meningitis, and gastroenteritis, primarily in immunocompromised individuals. although l. monocytogenes can usually be effectively treated with antibiotics, there is still around a 25% mortality rate with individuals who develop clinical listeriosis. neutrophils are innate immune cells required for the clearance of pathogenic organisms, including l. monocytog ... | 2016 | 27600509 |
| pyelonephritis with bacteremia caused by listeria monocytogenes: a case report. | listeria monocytogenes is a well-known cause of meningitis, colitis, and bacteremia; however, obstructive pyelonephritis caused by l. monocytogenes has never been reported. we herein report on a 90-year-old japanese woman with obstructive pyelonephritis and bacteremia due to uterus carcinoma invading the ureter. she was admitted to our hospital complaining of fever and chills, and her physical examination revealed left costovertebral angle tenderness. computed tomography showed hydronephrosis an ... | 2017 | 27600175 |
| molecular characterization and phylogenetic analysis of listeria monocytogenes isolated from milk and milk products in kaduna, nigeria. | in this study, listeria (l.) monocytogenes isolated from milk and milk products in kaduna, nigeria, were subjected to a multiplex pcr assay to identify virulence-associated genes (such as prf a, inl a, hly a, act a, and iap). of the 36 isolates, 9 (25%) were positive for one or two virulence-associated genes. based on the sample type, 6 (16.9%) of the isolates that possessed virulence-associated genes were obtained from raw milk, 2 (3.2%) from "manshanu," and 1 (2.8%) from "kindrimo." sequence a ... | 2016 | 27597873 |
| the diverse family of arp2/3 complexes. | the arp2/3 complex has so far been considered to be a single seven-subunit protein complex required for actin nucleation and actin filament polymerization in diverse critical cellular functions including phagocytosis, vesicular trafficking and lamellipodia extension. the arp2/3 complex is also exploited by bacterial pathogens and viruses during cellular infectious processes. recent studies suggest that some subunits of the complex are dispensable in specific cellular contexts, pointing to the ex ... | 2017 | 27595492 |
| neonatal listeriosis: uncommon or misdiagnosed? | the incidence of perinatal and neonatal listeriosis is underestimated due undiagnosed stillbirths, misdiagnosis of nl and underreporting of single case reports. recent outbreaks reinforce the need for better surveillance and targeted health education in certain population groups especially during pregnancy. | 2016 | 27589546 |
| epidermal fatty acid binding protein (e-fabp) is not required for the generation or maintenance of effector and memory t cells following infection with listeria monocytogenes. | following activation of naïve t cells there are dynamic changes in the metabolic pathways used by t cells to support both the energetic needs of the cell and the macromolecules required for growth and proliferation. among other changes, lipid metabolism undergoes dynamic transitions between fatty acid oxidation and fatty acid synthesis as cells progress from naïve to effector and effector to memory t cells. the hydrophobic nature of lipids requires that they be bound to protein chaperones within ... | 2016 | 27588422 |
| outbreak of listeria monocytogenes infections linked to a pasteurized ice cream product served to hospitalized patients. | two cases of hospital-acquired listeriosis were linked to a commercially produced, pasteurized ice cream mix. manufacturers should implement safety measures from the food safety modernization act to minimize the risk of listeria contamination. dietary guidelines for persons at high risk of listeriosis may need revision to recognize the potential risk from pasteurized products. | 2016 | 27586030 |
| factors contributes to spontaneous abortion caused by listeria monocytogenes, in tehran, iran, 2015. | spontaneous abortion is the loss of a fetus before the 20th week of pregnancy, when occurring naturally without any surgical or pharmaceutical intervention. on the other hand, listeria monocytogenes, as one of the foodborne pathogens, is a causative agent of listeriosis. the transfer of l. monocytogenes in pregnant women occurs as self-limited flu-like symptoms which may result in abortion, stillbirth or premature birth of infected infants. the purpose of this study was the identification of lis ... | 2016 | 27585255 |
| variability of listeria monocytogenes strains in biofilm formation on stainless steel and polystyrene materials and resistance to peracetic acid and quaternary ammonium compounds. | listeria monocytogenes is a foodborne pathogen able to tolerate adverse conditions by forming biofilms or by deploying stress resistant mechanisms, and thus manages to survive for long periods in food processing plants. this study sought to investigate the correlation between biofilm forming ability, tolerance to disinfectants and cell surface characteristics of twelve l. monocytogenes strains. the following attributes were evaluated: (i) biofilm formation by crystal violet staining method on po ... | 2016 | 27585076 |
| type i interferon promotes cell-to-cell spread of listeria monocytogenes. | type i interferons (ifns) play a critical role in antiviral immune responses, but can be deleterious to the host during some bacterial infections. listeria monocytogenes (lm) induces a type i ifn response by activating cytosolic antiviral surveillance pathways. this is beneficial to the bacteria as mice lacking the type i ifn receptor (ifnar1(-/-) ) are resistant to systemic infection by lm. the mechanisms by which type i ifns promote lm infection are unclear. here, we show that ifnar1 is requir ... | 2017 | 27582004 |
| spontaneous symmetry breaking for geometrical trajectories of actin-based motility in three dimensions. | actin-based motility is important for many cellular processes. in this article we extend our previous studies of an actin-propelled circular disk in two dimensions to an actin-propelled spherical bead in three dimensions. we find that for an achiral load the couplings between the motion of the load and the actin network induce a series of bifurcations, starting with a transition from rest to moving state, followed by a transition from straight to planar curves, and finally a further transition f ... | 2016 | 27575158 |
| oral exposure to listeria monocytogenes in aged il-17rko mice: a possible murine model to study listeriosis in susceptible populations. | foodborne listeria monocytogenes (lm) is a cause of serious illness and death in the us. the case-fatality rate of invasive lm infection in the elderly population is >50%. the goal of this study is to establish a murine model of oral lm infection that can be used as a surrogate for human foodborne listeriosis in the geriatric population. adult c57bl/6 (wild-type, wt) and adult or old il17r-ko (knock-out) mice were gavaged with a murinized lm strain (lmo-inla(m)) and monitored for body-weight los ... | 2016 | 27574777 |
| c-met inhibitors are potential novel therapeutic agents against listeria monocytogenes infection through blocking the bacteria entry into nonphagocytic cells. | high lethality of infections caused by listeria monocytogenes still remains a major clinical problem in spite of their susceptibility to a wide spectrum of antibiotics. the refractoriness towards treatment is primarily due to its amazing capacity to invade non-phagocytic cells and replicate there in, imparting the dual protection from immune response and antimicrobials. therefore, generating new anti-infective drugs against intracellular infections has emerged as an urgent issue in the therapeut ... | 2017 | 27572078 |
| dielectric barrier discharge atmospheric cold plasma inhibits escherichia coli o157:h7, salmonella, listeria monocytogenes, and tulane virus in romaine lettuce. | the present study investigated the effects of dielectric barrier discharge atmospheric cold plasma (dacp) treatment on the inactivation of escherichia coli o157:h7, salmonella, listeria monocytogenes, and tulane virus (tv) on romaine lettuce, assessing the influences of moisture vaporization, modified atmospheric packaging (map), and post-treatment storage on the inactivation of these pathogens. romaine lettuce was inoculated with e. coli o157:h7, salmonella, l. monocytogenes (~6logcfu/g lettuce ... | 2016 | 27562348 |
| notes from the field: outbreak of listeriosis associated with consumption of packaged salad - united states and canada, 2015-2016. | in september 2015, pulsenet, the national molecular subtyping network for foodborne disease surveillance, identified a cluster of listeria monocytogenes (listeria) clinical isolates indistinguishable by two-enzyme pulsed-field gel electrophoresis (pfge) pattern combination and highly related by whole-genome multilocus sequence typing (wgmlst). a case was defined as isolation of listeria with the outbreak pfge pattern and highly related by wgmlst with an isolation date on or after july 5, 2015, t ... | 2016 | 27559935 |
| serial magnetic resonance imaging findings of intracerebral spread of listeria utilising subcortical u-fibres and the extreme capsule. | we present a case of listeria monocytogenes cerebral abscess with axonal spread via the subcortical u-fibres and extreme capsule on magnetic resonance imaging, with follow-up studies demonstrating serial reduction in oedema and enhancement pattern of the white-matter fibre tracts following antimicrobial treatment. we discuss the microbiological mechanism of bacterial mobility to account for these unique imaging features. recognition of this distinct pattern of spread of l. monocytogenes cerebral ... | 2016 | 27558992 |
| modeling the behavior of listeria monocytogenes during enrichment in half fraser broth; impact of pooling and the duration of enrichment on the detection of l. monocytogenes in food. | a stochastic model describing the growth of listeria monocytogenes during enrichment in half fraser was developed for the purpose of estimating the effects of modifications to the first enrichment step of the en iso 11290-1 detection method. information pertaining to the variability of growth rates, physiological state of the cell, and the behavior of individual cells contaminating the food were obtained from previously published studies. we used this model to investigate the impact of pooling e ... | 2016 | 27554154 |
| influence of water activity on inactivation of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in peanut butter by microwave heating. | this study evaluated the efficacy of a 915 mhz microwave with 3 different electric power levels to inactivate three pathogens in peanut butter with different aw. peanut butter inoculated with escherichia coli o157:h7, salmonella enterica serovar typhimurium and listeria monocytogenes (0.3, 0.4, and 0.5 aw) were treated with a 915 mhz microwave with 2, 4, and 6 kw for up to 5 min. six kw 915 mhz microwave treatment for 5 min reduced these three pathogens by 1.97 to >5.17 log cfu/g. four kw 915 mh ... | 2016 | 27554151 |
| pregnane x receptor regulates pathogen-induced inflammation and host defense against an intracellular bacterial infection through toll-like receptor 4. | the nuclear pregnane x receptor (pxr) plays a central role in regulating xenobiotic metabolism. we now report a novel role for pxr as a critical negative regulator of innate immunity after infection. pxr(-/-) mice exhibited remarkably elevated pro-inflammatory cytokine and chemokine production following infection with listeria monocytogenes (lm). despite the more robust innate immune response, pxr(-/-) mice were highly susceptible to lm infection. surprisingly, disruption of the toll-like recept ... | 2016 | 27550658 |
| survival and growth of listeria monocytogenes on fresh-cut "athena" and "rocky ford" cantaloupes during storage at 4°c and 10°c. | cantaloupes, marketed as "rocky ford," were implicated in the u.s. multistate outbreak of listeriosis in 2011, which caused multiple fatalities. listeria monocytogenes can survive on whole cantaloupes and can be transferred to the flesh of melons. the growth of l. monocytogenes on fresh-cut "athena" and "rocky ford" cantaloupe cultivars during refrigerated storage was evaluated. fresh-cut cubes (16.4 cm(3)) from field-grown cantaloupes were each inoculated with 5 log10 cfu/ml of a multi-strain m ... | 2016 | 27548768 |
| morin inhibits biofilm production and reduces the virulence of listeria monocytogenes - an in vitro and in vivo approach. | the current study explores the in vitro and in vivo antibiofilm efficacy of morin against a leading foodborne pathogen-listeria monocytogenes (lm). minimum inhibitory concentration (mic) of morin against lm strains was found to be 100μg/ml. the non-antibacterial effect of morin at its sub-mics (6.25, 12.5 and 25μg/ml) was determined through growth curve and xtt assay. morin at its sub-mics demonstrated a significant dose dependent inhibitory efficacy against lm biofilm formation which was also e ... | 2016 | 27543817 |
| the four-component aureocin a70 as a promising agent for food biopreservation. | aureocin a70 is the only four-component bacteriocin described to date. as it inhibits the growth of a wide range of gram-positive bacteria, including listeria monocytogenes strains isolated from food, its potential for improving food safety was investigated in this study. aureocin a70 (10,240au/ml) proved to be bactericidal, but not extensively lytic, against listerial strains. the antibacterial activity of aureocin a70 (16au/ml) was then tested in uht-treated skimmed milk inoculated with the fo ... | 2016 | 27543814 |
| the essential role of neutrophils during infection with the intracellular bacterial pathogen listeria monocytogenes. | neutrophils have historically been characterized as first responder cells vital to host survival because of their ability to contain and eliminate bacterial and fungal pathogens. however, recent studies have shown that neutrophils participate in both protective and detrimental responses to a diverse array of inflammatory and infectious diseases. although the contribution of neutrophils to extracellular infections has been investigated for decades, their specific role during intracellular bacteri ... | 2016 | 27543669 |
| predictive risk factors for listeria monocytogenes meningitis compared to pneumococcal meningitis: a multicenter case-control study. | various immunocompromised conditions increase the risk of meningitis caused by listeria monocytogenes. however, the relative importance of these risk factors has not been well established. we determined the risk factors that predict meningitis due to l. monocytogenes compared to that caused by streptococcus pneumoniae. | 2017 | 27541039 |
| cryptococcus gattii meningitis complicated by listeria monocytogenes infection. | | 2016 | 27532498 |
| achillea schurii flowers: chemical, antioxidant, and antimicrobial investigations. | this study aims to evaluate the phenolic profile, and antioxidant and antimicrobial activity of achillea schurii sch.-bip., an endemic species from romania that has not been investigated yet. the chromatographic profile of the phenolic components was obtained using the hplc-ms method, while the total polyphenol, flavonoid, caffeic acid derivative contents were quantified using spectrophotometric methods. the antioxidant activity was evaluated using different methods: dpph radical scavenging, hem ... | 2016 | 27529204 |
| antifungal and antibacterial activities of petroselinum crispum essential oil. | parsley [petroselinum crispum (mill.) fuss] is regarded as an aromatic, culinary, and medicinal plant and is used in the cosmetic, food, and pharmaceutical industries. however, few studies with conflicting results have been conducted on the antimicrobial activity of parsley essential oil. in addition, there have been no reports of essential oil obtained from parsley aerial parts, except seeds, as an alternative natural antimicrobial agent. also, microorganism resistance is still a challenge for ... | 2016 | 27525894 |
| adhesion- and degranulation-promoting adapter protein promotes cd8 t cell differentiation and resident memory formation and function during an acute infection. | during acute infections, naive ag-specific cd8 t cells are activated and differentiate into effector t cells, most of which undergo contraction after pathogen clearance. a small population of cd8 t cells persists as memory to protect against future infections. we investigated the role of adhesion- and degranulation-promoting adapter protein (adap) in promoting cd8 t cell responses to a systemic infection. naive ag-specific cd8 t cells lacking adap exhibited a modest expansion defect early after ... | 2016 | 27521337 |
| core genome multilocus sequence typing for identification of globally distributed clonal groups and differentiation of outbreak strains of listeria monocytogenes. | many listeriosis outbreaks are caused by a few globally distributed clonal groups, designated clonal complexes or epidemic clones, of listeria monocytogenes, several of which have been defined by classic multilocus sequence typing (mlst) schemes targeting 6 to 8 housekeeping or virulence genes. we have developed and evaluated core genome mlst (cgmlst) schemes and applied them to isolates from multiple clonal groups, including those associated with 39 listeriosis outbreaks. the cgmlst clusters we ... | 2016 | 27520821 |
| listeria monocytogenes induces mast cell extracellular traps. | mast cells play an essential role in different immunological phenomena including allergy and infectious diseases. several bacteria induce mast cell activation leading to degranulation and the production of several cytokines and chemokines. however, mast cells also have different microbicidal activities such as phagocytosis and the release of dna with embedded granular proteins known as mast cell extracellular traps (mcets). although previous reports indicate that extracellular bacteria are able ... | 2017 | 27520114 |
| influence of environmental factors on phage-bacteria interaction and on the efficacy and infectivity of phage p100. | when using bacteriophages to control food-borne bacteria in food production plants and processed food, it is crucial to consider that environmental conditions influence their stability. these conditions can also affect the physiological state of bacteria and consequently host-virus interaction and the effectiveness of the phage ability to reduce bacteria numbers. in this study we investigated the stability, binding, and replication capability of phage p100 and its efficacy to control listeria mo ... | 2016 | 27516757 |
| review of prosthetic joint infection from listeria monocytogenes. | prosthetic joint infection from listeria monocytogenes is rare. we decided to shed light on this illness and review the reported cases to better understand its characteristics. | 2016 | 27513716 |
| impact of food model (micro)structure on the microbial inactivation efficacy of cold atmospheric plasma. | the large potential of cold atmospheric plasma (cap) for food decontamination has recently been recognized. room-temperature gas plasmas can decontaminate foods without causing undesired changes. this innovative technology is a promising alternative for treating fresh produce. however, more fundamental studies are needed before its application in the food industry. the impact of the food structure on cap decontamination efficacy of salmonella typhimurium and listeria monocytogenes was studied. c ... | 2017 | 27507138 |
| engineering a prototypic p-type atpase listeria monocytogenes ca(2+)-atpase 1 for single-molecule fret studies. | approximately 30% of the atp generated in the living cell is utilized by p-type atpase primary active transporters to generate and maintain electrochemical gradients across biological membranes. p-type atpases undergo large conformational changes during their functional cycle to couple atp hydrolysis in the cytoplasmic domains to ion transport across the membrane. the ca(2+)-atpase from listeria monocytogenes, lmca1, was found to be a suitable model of p-type atpases and was engineered to facili ... | 2016 | 27501274 |
| human placental and decidual organ cultures to study infections at the maternal-fetal interface. | the placenta shows a large degree of interspecies anatomic variability. to best understand biology and pathophysiology of the human placenta, it is imperative to design experiments using human cells and tissues. an advantage of organ culture is maintenance of three-dimensional (3d) structural organization and extracellular matrix. the goal of the method described here is successful establishment of ex vivo human gestational tissue organ cultures and their healthy culture maintenance for 72-96 hr ... | 2016 | 27500727 |
| evolution and diversity of listeria monocytogenes from clinical and food samples in shanghai, china. | listeria monocytogenes is a significant foodborne pathogen causing severe systemic infections in humans with high mortality rates. the objectives of this work were to establish a phylogenetic framework of l. monocytogenes from china and to investigate sequence diversity among different serotypes. we selected 17 l. monocytogenes strains recovered from patients and foods in china representing serotypes 1/2a, 1/2b, and 1/2c. draft genome sequences were determined using illumina miseq technique and ... | 2016 | 27499751 |
| internalization of listeria monocytogenes in whole avocado. | in recent years, tree fruits have emerged as a new concern for listeria monocytogenes contamination. the objective of the current study was to evaluate the potential internalization of l. monocytogenes from the surface of avocados into the edible portions of the fruit during certain postharvest practices simulated in a laboratory setting. one set of intact avocados was spot inoculated with l. monocytogenes on the stem scar, and the second set was hydrocooled in water contaminated with l. monocyt ... | 2016 | 27497134 |
| inhibition of listeria monocytogenes by buffered dry vinegar in reduced-sodium ready-to-eat uncured turkey stored at 4°c. | a reduced-sodium ready-to-eat (rte) uncured turkey was manufactured with buffered dry vinegar treatments to validate the inhibition of listeria monocytogenes and spoilage microflora and to determine the effects on sensory and quality attributes. samples were stored at 4°c for 12 weeks, and the study was independently replicated three times. two different five-strain inocula of l. monocytogenes obtained from different sources were used for evaluating the efficacy of the buffered dry vinegar treat ... | 2016 | 27497127 |
| effect of hydrogen peroxide in combination with minimal thermal treatment for reducing bacterial populations on cantaloupe rind surfaces and transfer to fresh-cut pieces. | surface structure and biochemical characteristics of bacteria and produce play a major role in how and where bacteria attach, complicating decontamination treatments. whole cantaloupe rind surfaces were inoculated with salmonella, escherichia coli o157:h7, and listeria monocytogenes at 10(7) cfu/ml. average population size of salmonella, escherichia coli o157:h7, and l. monocytogenes recovered after surface inoculation was 4.8 ± 0.12, 5.1 ± 0.14, and 3.6 ± 0.13 log cfu/cm(2), respectively. inocu ... | 2016 | 27497118 |
| quantitative image analysis to characterize the dynamics of listeria monocytogenes biofilms. | this work shows that the combination of two-dimensional (2d) and three-dimensional (3d) analyses of images acquired by confocal laser scanning microscopy facilitates the quantitative spatiotemporal characterization of architectures formed by listeria monocytogenes biofilms. in particular, the analysis of structural features such as maximum thickness, biovolume, areal porosity and maximum diffusion distance allowed elucidating differences in biofilm formation of three l. monocytogenes strains (l1 ... | 2016 | 27494720 |
| isolation and characterization of atypical listeria monocytogenes associated with a canine urinary tract infection. | listeria monocytogenes, a well-described cause of encephalitis and abortion in ruminants and of food-borne illness in humans, is rarely associated with disease in companion animals. a case of urinary tract infection associated with an atypical, weakly hemolytic l. monocytogenes strain is described in a diabetic dog. the serotype of the l. monocytogenes isolate was determined to be 1/2a (3a), with the multilocus genotyping pattern 2.72_1/2a. a nucleotide substitution (gly145asp) was detected at r ... | 2016 | 27493137 |
| genome sequence of listeria monocytogenes strain hpb2088 (serotype 1/2a), an environmental isolate collected in canada in 1994. | listeria monocytogenes is a foodborne pathogen that causes severe illness. thus, ongoing efforts at real-time whole-genome sequencing are of utmost importance. however, it is also important that retrospective analyses that place these data into context be performed. here, we present the genome sequence of strain hpb2088, which was collected in 1994. | 2016 | 27491990 |
| listeria monocytogenes inhibits serotonin transporter in human intestinal caco-2 cells. | listeria monocytogenes is a gram-positive bacterium that can cause a serious infection. intestinal microorganisms have been demonstrated to contribute to intestinal physiology not only through immunological responses but also by modulating the intestinal serotonergic system. serotonin (5-ht) is a neuromodulator that is synthesized in the intestinal epithelium and regulates the whole intestinal physiology. the serotonin transporter (sert), located in enterocytes, controls intestinal 5-ht availabi ... | 2016 | 27488594 |
| modeling the effect of temperature on survival rate of listeria monocytogenes in yogurt. | the aim of the study was to (i) evaluate the behavior of listeria monocytogenes in a commercially produced yogurt, (ii) determine the survival/inactivation rates of l. monocytogenes during cold storage of yogurt and (iii) to generate primary and secondary mathematical models to predict the behavior of these bacteria during storage at different temperatures. the samples of yogurt were inoculated with the mixture of three l. monocytogenes strains and stored at 3, 6, 9, 12 and 15°c for 16 days. the ... | 2016 | 27487505 |
| detection of listeria monocytogenes in csf from three patients with meningoencephalitis by next-generation sequencing. | encephalitis caused by listeria monocytogenes (l. monocytogenes) is rare but sometimes fatal. early diagnosis is difficult using routine cerebrospinal fluid (csf) tests, while next-generation sequencing (ngs) is increasingly being used for the detection and characterization of pathogens. | 2016 | 27486935 |
| quality attributes and microbial survival on whole cantaloupes with antimicrobial coatings containing chitosan, lauric arginate, cinnamon oil and ethylenediaminetetraacetic acid. | cantaloupes are susceptible to microbiological contamination in pre- or postharvest environments. novel intervention strategies, such as antimicrobial coatings, are needed to improve the microbiological safety of cantaloupes. the objective of this study was to prepare whole cantaloupes coated with mixtures containing chitosan, lauric arginate (lae), cinnamon oil (co), and ethylenediaminetetraacetic acid (edta) and determine survival characteristics of inoculated foodborne pathogens during storag ... | 2016 | 27484251 |
| genotypic and virulence characteristics of listeria monocytogenes recovered from food items in lebanon. | listeria monocytogenes is the agent of listeriosis, a life threatening foodborne disease for immunocompromised patients and pregnant women. this bacterium is not routinely screened for in lebanon and there is lack of data about the prevalent strains and their potential pathogenicity. to that purpose, this study was undertaken to characterize l. monocytogenes from various food products, by assessing the in vitro biofilm forming ability, detecting their virulence potential, and characterizing them ... | 2016 | 27482802 |
| secretion chaperones prsa2 and htra are required for listeria monocytogenes replication following intracellular induction of virulence factor secretion. | the gram-positive bacterium listeria monocytogenes transitions from an environmental organism to an intracellular pathogen following its ingestion by susceptible mammalian hosts. bacterial replication within the cytosol of infected cells requires activation of the central virulence regulator prfa followed by a prfa-dependent induction of secreted virulence factors. the prfa-induced secreted chaperone prsa2 and the chaperone/protease htra contribute to the folding and stability of select proteins ... | 2016 | 27481256 |
| effect of nisin and perilla oil combination against listeria monocytogenes and staphylococcus aureus in milk. | in the present study, in vitro interaction of nisin and perilla oil (po) against 20 food-borne isolates of l. monocytogenes and s. aureus were assessed using a checkerboard microdilution method. synergism was observed in tested strains with the fractional inhibitory concentration indexs (ficis) ranges from 0.125-0.25 and 0.19-0.375, respectively. scanning electron microscopy was carried out to investigate the effect of nisin and po on the integrity of cell wall and membrane of l. monocytogenes a ... | 2016 | 27478220 |
| antilisterial effects of antibacterial formulations containing essential oils, nisin, nitrite and organic acid salts in a sausage model. | this study was conducted to evaluate the effects of sixteen antibacterial formulations against listeria monocytogenes in a sausage model using a standard experimental design with 4 independent factors at 2 levels (2(4)). four independent factors consisted of nisin (12.5-25 ppm), nitrite (100-200 ppm) and organic acid salts (1.55-3.1 %) and the mixture of chinese cinnamon and cinnamon bark essential oils (eos) (0.025-0.05 %). based on the analysis, utilization of low (0.025 %) or high concentrati ... | 2016 | 27478218 |
| fast and sensitive detection of foodborne pathogen using electrochemical impedance analysis, urease catalysis and microfluidics. | early screening of pathogenic bacteria is a key to prevent and control of foodborne diseases. in this study, we developed a fast and sensitive bacteria detection method integrating electrochemical impedance analysis, urease catalysis with microfluidics and using listeria as model. the listeria cells, the anti-listeria monoclonal antibodies modified magnetic nanoparticles (mnps), and the anti-listeria polyclonal antibodies and urease modified gold nanoparticles (aunps) were incubated in a fluidic ... | 2016 | 27476059 |
| antimicrobial resistance, virulence profile, and molecular characterization of listeria monocytogenes isolated from ready-to-eat food in china, 2013-2014. | we aimed to investigate the potential pathogenic profile and antibiotic resistance of listeria monocytogenes isolated from ready-to-eat food in china. antimicrobial resistance was determined by broth microdilution following the clinical and laboratory standards institute protocol. molecular serotyping, virulence, and resistance genes were identified using pcr. multi-locus sequence typing was performed on resistant strains. a total of 11.53% (113/980) isolates were resistant, from which 82.3% (93 ... | 2016 | 27470106 |
| nitrite formation from vegetable sources and its use as a preservative in cooked sausage. | due to the potential health risk associated with nitrites, nitrite alternatives from natural sources in meat products have been investigated. we compared the nitrate contents of young radish, lettuce and commercial vegetable powder (cabbage and chinese cabbage). we also investigated the effect of incubation time and salt addition on vegetable nitrite formation from vegetable sources. the antioxidant and antimicrobial effects of vegetable nitrite in cooked sausage were also compared with sodium n ... | 2017 | 27469979 |
| mycotic abdominal aortic aneurysm due to listeria monocytogenes. | | 2017 | 27469417 |
| biofilm formation and disinfectant susceptibility of persistent and nonpersistent listeria monocytogenes isolates from gorgonzola cheese processing plants. | the aim of this study was to investigate whether the biofilm-forming ability and/or the disinfectant susceptibility accounted for the persistence of listeria monocytogenes in gorgonzola cheese processing plants. for this purpose, a set of 16 l. monocytogenes isolates collected in the 2004-2007 period was analyzed, including 11 persistent isolates collected in different years, within the collection period, and displaying identical or highly correlated pulsotypes. the evaluation of biofilm-forming ... | 2016 | 27462729 |
| differentiation of distinct long-lived memory cd4 t cells in intestinal tissues after oral listeria monocytogenes infection. | mucosal antigen-specific cd4 t-cell responses to intestinal pathogens remain incompletely understood. here we examined the cd4 t-cell response after oral infection with an internalin a 'murinized' listeria monocytogenes (lm). oral lm infection induced a robust endogenous listeriolysin o (llo)-specific cd4 t-cell response with distinct phenotypic and functional characteristics in the intestine. circulating llo-specific cd4 t cells transiently expressed the 'gut-homing' integrin α4β7 and accumulat ... | 2017 | 27461178 |
| the small colony variant of listeria monocytogenes is more tolerant to antibiotics and has altered survival in raw 264.7 murine macrophages. | small colony variant (scv) cells of bacteria are a slow-growing phenotype that result from specific defects in the electron transport chain. they form pinpoint colonies on agar plates and have a variety of phenotypic characteristics, such as altered carbon metabolism, decreased toxin and lytic enzyme production, aminoglycoside resistance, and increased intracellular persistence. they are clinically relevant in staphylococcus aureus and pseudomonas aeruginosa, serving as a reservoir for recurrent ... | 2016 | 27458449 |
| purification and characterization of a rabbit serum factor that kills listeria species and other foodborne bacterial pathogens. | in an in-vitro assay, rabbit serum, but not human serum, killed listeria monocytogenes, a foodborne pathogen. the aim of our study was to purify and partially characterize this killing factor. listericidin was purified from rabbit serum by a single-step ion-exchange chromatography with deae-sephadex a-50 and its antimicrobial activity was assessed by a microdilution method. listericidin is a protein with a molecular weight of 9 kda and an isoelectric point of 8.1. it kills l. monocytogenes at 4° ... | 2016 | 27455064 |
| artemisia arborescens essential oil composition, enantiomeric distribution, and antimicrobial activity from different wild populations from the mediterranean area. | aerial parts of artemisia arborescens were collected from different sites of the mediterranean area (southwestern algeria and southern italy) and the chemical composition of their essential oil (eo) extracted by hydrodistillation was studied by both gas chromatography (gc) equipped with an enantioselective capillary column and gc/mass spectrometry (gc/ms). the eos obtained were tested against several listeria monocytogenes strains. using gc and gc/ms, 41 compounds were identified, accounting for ... | 2016 | 27447740 |
| inactivation of salmonella enterica and listeria monocytogenes in cantaloupe puree by high hydrostatic pressure with/without added ascorbic acid. | the objective of this research was to evaluate and develop a method for inactivation of salmonella enterica and listeria monocytogenes in cantaloupe puree (cp) by high hydrostatic pressure (hhp). cantaloupe being the most netted varieties of melons presents a greater risk of pathogen transmission. freshly prepared cp with or without 0.1% ascorbic acid (aa) was inoculated with a bacterial cocktail composed of a three serotype mixture of s. enterica (s. poona, s. newport h1275 and s. stanley h0558 ... | 2016 | 27441819 |
| capture and concentration of viral and bacterial foodborne pathogens using apolipoprotein h. | the need for improved pathogen separation and concentration methods to reduce time-to-detection for foodborne pathogens is well recognized. apolipoprotein h (apoh) is an acute phase human plasma protein that has been previously shown to interact with viruses, lipopolysaccharides (lps) and bacterial proteins. the purpose of this study was to determine if apoh was capable of binding and efficiently capturing two representative human norovirus strains (gi.1 and gii.4), a cultivable surrogate, and f ... | 2016 | 27439140 |
| listeria monocytogenes cross-contamination of cheese: risk throughout the food supply chain. | listeria monocytogenes has been the most common microbial cause of cheese-related recalls in both the united states and canada in recent years. since l. monocytogenes is inactivated by pasteurization, the majority of these cases have been linked to environmental and cross-contamination of fresh-soft, soft-ripened, and semi-soft cheeses. cross-contamination of foods with l. monocytogenes is a continuous risk throughout the food supply chain and presents unique challenges for subsequent illness an ... | 2016 | 27435307 |
| growth differences and competition between listeria monocytogenes strains determine their predominance on ham slices and lead to bias during selective enrichment with the iso protocol. | listeria monocytogenes strains are widespread in the environment where they live well mixed, often resulting in multiple strains contaminating a single food sample. the occurrence of different strains in the same food might trigger strain competition, contributing to uneven growth of strains in food and to bias during selective procedures. we tested the growth of seven l. monocytogenes strains (c5, 6179, scotta, pl24, pl25, pl26, pl27) on ham slices and on nutrient-rich agar at 10°c, singly and ... | 2016 | 27434679 |
| application of lemongrass oil-containing polylactic acid films to the packaging of pork sausages. | polylactic acid (pla) is a biodegradable and renewable polymer, which represents a valuable alternative to plastic packaging films, often associated with environmental problems. in this study, we tested the suitability of pla as a biodegradable packaging film and assessed the antimicrobial activity of lemongrass oil (lo), incorporated into the pla film in different concentrations. to obtain the optimal physical properties for pla films, tensile strength, elongation at break, and water vapor perm ... | 2016 | 27433114 |
| listeria rhombencephalitis mimicking a demyelinating event in an immunocompetent young patient. | listeriosis caused by listeria monocytogenes (lm) is a potentially lethal foodborne infection of the central nervous system (cns) and the third most common cause of bacterial meningitis. foods most commonly implicated are soft cheeses, raw or ready-to-eat meat and pre-processed foods. the incubation time is between 11 and 70 days. rarely lm rhombencephalitis (re) can occur, which typically has a biphasic course with non- specific prodromal symptoms like fever, malaise, fatigue, headache, nausea ... | 2017 | 27430683 |
| listeria monocytogenes endophthalmitis - case report and review of risk factors and treatment outcomes. | the majority of cases of endophthalmitis are caused by exogenous pathogens; only 5-10 % are of endogenous origin. one cause of these rare cases of endogenous endophthalmitis is listeria monocytogenes. twenty-six cases of endophthalmitis due to this pathogen have been published over the last twenty years. the aim of this review is to summarize the main risk factors and common clinical findings of endogenous endophthalmitis due to listeria monocytogenes. | 2016 | 27424034 |
| three novel c1q domain containing proteins from the disk abalone haliotis discus discus: genomic organization and analysis of the transcriptional changes in response to bacterial pathogens. | the globular c1q (gc1q) domain containing proteins, commonly referred as c1q domain containing (c1qdc) proteins, are an essential family of proteins involved in various innate immune responses. in this study, three novel c1qdc proteins were identified from the disk abalone (haliotis discus discus) transcriptome database and designated as abc1qdc1, abc1qdc2, and abc1qdc3. the cdna sequences of abc1qdc1, abc1qdc2, and abc1qdc3 consisted of 807, 1305, and 660 bp open reading frames (orfs) encoding ... | 2016 | 27417231 |