| a20 curtails primary but augments secondary cd8(+) t cell responses in intracellular bacterial infection. | the ubiquitin-modifying enzyme a20, an important negative feedback regulator of nf-κb, impairs the expansion of tumor-specific cd8(+) t cells but augments the proliferation of autoimmune cd4(+) t cells. to study the t cell-specific function of a20 in bacterial infection, we infected t cell-specific a20 knockout (cd4-cre a20(fl/fl)) and control mice with listeria monocytogenes. a20-deficient pathogen-specific cd8(+) t cells expanded stronger resulting in improved pathogen control at day 7 p.i. im ... | 2016 | 28004776 |
| surface-enhanced raman spectroscopy introduced into the international standard organization (iso) regulations as an alternative method for detection and identification of pathogens in the food industry. | we show that surface-enhanced raman spectroscopy (sers) coupled with principal component analysis (pca) can serve as a fast, reliable, and easy method for detection and identification of food-borne bacteria, namely salmonella spp., listeria monocytogenes, and cronobacter spp., in different types of food matrices (salmon, eggs, powdered infant formula milk, mixed herbs, respectively). the main aim of this work was to introduce the sers technique into three iso (6579:2002; 11290-1:1996/a1:2004; 22 ... | 2017 | 28004171 |
| comparative genomics analyses revealed two virulent listeria monocytogenes strains isolated from ready-to-eat food. | listeria monocytogenes is an important foodborne pathogen that causes considerable morbidity in humans with high mortality rates. in this study, we have sequenced the genomes and performed comparative genomics analyses on two strains, lm115 and lm41, isolated from ready-to-eat food in malaysia. | 2016 | 27999619 |
| benzalkonium tolerance genes and outcome in listeria monocytogenes meningitis. | listeria monocytogenes is a food-borne pathogen that can cause meningitis. the listerial genotype st6 has been linked to increasing rates of unfavourable outcome over time. we investigated listerial genetic variation and the relation with clinical outcome in meningitis. | 2017 | 27998823 |
| a systematic investigation of parameters influencing droplet rain in the listeria monocytogenes prfa assay - reduction of ambiguous results in ddpcr. | the droplet digital polymerase chain reaction (ddpcr) determines dna amounts based upon the pattern of positive and negative droplets, according to poisson distribution, without the use of external standards. however, division into positive and negative droplets is often not clear because a part of the droplets has intermediate fluorescence values, appearing as "rain" in the plot. despite the droplet rain, absolute quantification with ddpcr is possible, as shown previously for the prfa assay in ... | 2016 | 27992475 |
| insight into the genome of brochothrix thermosphacta, a problematic meat spoilage bacterium. | brochothrix thermosphacta is a dominant but poorly studied meat spoilage organism. it is a close relative of the foodborne pathogen listeria monocytogenes, and brochothrix constitutes the second genus in the listeriaceae family. here, the genomes of 12 b. thermosphacta strains were sequenced, assembled into draft genomes, characterized, and compared with the genomes of brochothrix campestris and l. monocytogenes phenotypic properties including biogenic amine production and antibiotic and heavy m ... | 2017 | 27986732 |
| novel cadmium resistance determinant in listeria monocytogenes. | listeria monocytogenes is a foodborne pathogen that can cause severe disease (listeriosis) in susceptible individuals. it is ubiquitous in the environment and often exhibits resistance to heavy metals. one of the determinants that enables listeria to tolerate exposure to cadmium is the cadac efflux system, with cada being a p-type atpase. three different cada genes (designated cada1 to cada3) were previously characterized in l. monocytogenes a novel putative cadmium resistance gene (cada4) was r ... | 2017 | 27986731 |
| production of bacterial ghosts from gram-positive pathogen listeria monocytogenes. | the gram-positive bacterium listeria monocytogenes is a ubiquitous intracellular pathogen, which has been implicated within the past decade as the causative organism in several outbreaks of foodborne disease. bacterial ghosts (bgs) are nonliving and vacant cell envelopes of bacteria generated by releasing the bacterial cytoplasm through a channel in the cell envelope. this study attempted to produce gram-positive pathogenic l. monocytogenes ghosts (lmgs) with simple chemicals. | 2017 | 27982711 |
| involvement of il-17a-producing tcr γδ t cells in late protective immunity against pulmonary mycobacterium tuberculosis infection. | interleukin (il)-17a is a cytokine originally reported to induce neutrophil-mediated inflammation and anti-microbial activity. the cd4(+) t cells, which produce il-17a, have been well characterized as th17 cells. on the other hand, il-17a-producing tcr γδ(+) t cells have been reported to participate in the immune response at an early stage of infection with listeria monocytogenes and mycobacterium bovis in mice. however, the involvement of il-17a in protective immunity was not clearly demonstrat ... | 2016 | 27980775 |
| prevalence and antibiotic susceptibility profiles of listeria monocytogenes contamination of chicken flocks and meat in oyo state, south-western nigeria: public health implications. | food contamination with listeria monocytogenes is on the increase posing threats to public health with growing trends in food products recalls due to suspected listeria contamination. | 2016 | 27980380 |
| successful treatment of refractory listeria meningitis and bacteremia with trimethoprim-sulfamethoxazole in an immunocompetent child. | listeria monocytogenes is an important cause of life-threatening bacteremia and meningoencephalitis in neonates, pregnant women, the elderly, and immunocompromised individuals. however, it is an uncommon cause of illness in immunocompetent children beyond the neonatal period. ampicillin with or without an aminoglycoside remains the best treatment for listeriosis. here, we report a rare case of listeria meningitis and bacteremia in a 7-month-old immunocompetent girl, which was refractory to ampic ... | 2016 | 27976567 |
| biomarker tools to design clinical vaccines determined from a study of annual listeriosis incidence in northern spain. | two regions of northern spain, gipuzkoa, and cantabria present high annual incidence of listeriosis (1.86 and 1.71 cases per 100,000 inhabitants, respectively). we report that the high annual incidences are a consequence of infection with highly virulent listeria monocytogenes isolates linked to fatal outcomes in elderly patients with cancer. in addition, listeriosis patients with cancer present low il-17a/il-6 ratios and significantly reduced levels of anti-gapdh1-22 antibodies, identified as t ... | 2016 | 27965668 |
| a bioengineered nisin derivative, m21a, in combination with food grade additives eradicates biofilms of listeria monocytogenes. | the burden of foodborne disease has large economic and social consequences worldwide. despite strict regulations, a number of pathogens persist within the food environment, which is greatly contributed to by a build-up of resistance mechanisms and also through the formation of biofilms. biofilms have been shown to be highly resistant to a number of antimicrobials and can be extremely difficult to remove once they are established. in parallel, the growing concern of consumers regarding the use of ... | 2016 | 27965658 |
| polyethylene oxide (peo)-hyaluronic acid (ha) nanofibers with kanamycin inhibits the growth of listeria monocytogenes. | listeria monocytogenes is well known to cause prosthetic joint infections in immunocompromised patients. in this study, polyethylene oxide (peo) nanofibers, containing kanamycin and hyaluronic acid (ha), were prepared by electrospinning at a constant electric field of 10kv. peo nanofibers spun with 0.2% (w/v) ha and 1% (w/v) kanamycin had a smooth, bead-free structure at 30-35% relative humidity. the average diameter of the nanofibers was 83±20nm. attenuated total reflectance (atr)-fourier trans ... | 2017 | 27960136 |
| utilization of multiple substrates by butyrate kinase from listeria monocytogenes. | listeria monocytogenes, the causative agent of listeriosis, can build up to dangerous levels in refrigerated foods potentially leading to expensive product recalls. an important aspect of the bacterium's growth at low temperatures is its ability to increase the branched-chain fatty acid anteiso c15:0 content of its membrane at lower growth temperatures, which imparts greater membrane fluidity. mutants in the branched-chain α-keto dehydrogenase (bkd) complex are deficient in branched-chain fatty ... | 2017 | 27940001 |
| inflammation-induced cd69(+) kupffer cell feedback inhibits t cell proliferation via membrane-bound tgf-β1. | kupffer cells, tissue-resident macrophage lineage cell, are enriched in vertebrate liver. the mouse f4/80(+) kupffer cells have been subclassified into two subpopulations according to their phenotype and function: cd68(+) subpopulation with potent reactive oxygen species (ros) production and phagocytic capacities, and cd11b(+) subpopulation with a potent capacity to produce t helper 1 cytokines. in addition, cd11b(+) kupffer cells/macrophages may be migrated from the bone marrow or spleen, espec ... | 2016 | 27933593 |
| the role of stress and stress adaptations in determining the fate of the bacterial pathogen listeria monocytogenes in the food chain. | the foodborne pathogen listeria monocytogenes is a highly adaptable organism that can persist in a wide range of environmental and food-related niches. the consumption of contaminated ready-to-eat foods can cause infections, termed listeriosis, in vulnerable humans, particularly those with weakened immune systems. although these infections are comparatively rare they are associated with high mortality rates and therefore this pathogen has a significant impact on food safety. l. monocytogenes can ... | 2016 | 27933042 |
| draft genome sequences of two historical listeria monocytogenes strains from human listeriosis cases in 1933. | we report here the draft genome sequences of two listeria monocytogenes strains from some of the earliest reported cases of human listeriosis in north america. the strains were isolated in 1933 from patients in massachusetts and connecticut, usa, and belong to the widely disseminated hypervirulent clonal complex 1 (cc1) and cc2. | 2016 | 27932656 |
| brain abscess due to listeria monocytogenes after hellp syndrome in a patient with antiphospholipid syndrome. | to illustrate an unusual case of listeria cerebral abscess. | 2016 | 27927881 |
| innate immunity based cancer immunotherapy: b16-f10 murine melanoma model. | using killed microorganisms or their parts to stimulate immunity for cancer treatment dates back to the end of 19(th) century. since then, it undergone considerable development. our novel approach binds ligands to the tumor cell surface, which stimulates tumor phagocytosis. the therapeutic effect is further amplified by simultaneous application of agonists of toll-like receptors. we searched for ligands that induce both a strong therapeutic effect and are safe for humans. | 2016 | 27927165 |
| molecular identification and functional delineation of a glutathione reductase homolog from disk abalone (haliotis discus discus): insights as a potent player in host antioxidant defense. | glutathione reductase (gsr) is an enzyme that catalyzes the biochemical conversion of oxidized glutathione (gssg) into the reduced form (gsh). since the ratio between the two forms of glutathione (gsh/gssg) is important for the optimal function of gsh to act as an antioxidant against h2o2, the contribution of gsr as an enzymatic regulatory agent to maintain the proper ratio is essential. abalones are marine mollusks that frequently encounter environmental factors that can trigger the overproduct ... | 2017 | 27919756 |
| tracking listeria monocytogenes contamination and virulence-associated characteristics in the ready-to-eat meat-based food products industry according to the hygiene level. | listeria monocytogenes isolates collected from final products and food contact surfaces of 10 ready-to-eat meat-based food products (rtemp) producing industries were analyzed to relate their virulence-associated characteristics and genetic profiles with the hygiene assessment of those industries. together with sample collection, an audit was performed to evaluate the implemented food safety management system and to investigate the specific audit requisites more associated to the occurrence of th ... | 2017 | 27919005 |
| assessing vacuolar escape of listeria monocytogenes. | listeria monocytogenes is a bacterial pathogen which invades and multiplies within non-professional phagocytes. signaling cascades involved in cellular entry have been extensively analyzed, but the events leading to vacuolar escape remain less clear. in this chapter, we detail a microscopy fret-based assay which allows quantitatively measuring l. monocytogenes infection and escape from its internalization vacuole, as well as a correlative light/electron microscopy method to investigate the morph ... | 2017 | 27914079 |
| experimental infection with listeria monocytogenes as a model for studying host interferon-γ responses. | l. monocytogenes is a gram-positive bacterium that is a cause of food borne disease in humans. experimental infection of mice with this pathogen has been highly informative on the role of innate and adaptive immune cells and specific cytokines in host immunity against intracellular pathogens. production of ifn-γ by innate cells during sublethal infection with l. monocytogenes is important for activating macrophages and early control of the pathogen(1-3). in addition, ifn-γ production by adaptive ... | 2016 | 27911410 |
| the majority of genotypes of the virulence gene inla are intact among natural watershed isolates of listeria monocytogenes from the central california coast. | internalin a is an essential virulence gene involved in the uptake of the foodborne pathogen listeria monocytogenes into host cells. it is intact in clinical strains and often truncated due to premature stop codons (pmscs) in isolates from processed foods and processing facilities. less information is known about environmental isolates. we sequenced the inla alleles and did multi locus variable number tandem repeat analysis (mlva) on 112 l. monocytogenes isolates from a 3-year period from natura ... | 2016 | 27907153 |
| assay for listeria monocytogenes cells in whole blood using isotachophoresis and recombinase polymerase amplification. | we present a new approach which enables lysis, extraction, and detection of inactivated listeria monocytogenes cells from blood using isotachophoresis (itp) and recombinase polymerase amplification (rpa). we use an itp-compatible alkaline and proteinase k approach for rapid and effective lysis. we then perform itp purification to separate bacterial dna from whole blood contaminants using a microfluidic device that processes 25 μl sample volume. lysis, mixing, dispensing, and on-chip itp purifica ... | 2016 | 27904893 |
| effect of inoculum size, bacterial species, type of surfaces and contact time to the transfer of foodborne pathogens from inoculated to non-inoculated beef fillets via food processing surfaces. | the objective of the present study was to determine the factors affecting the transfer of foodborne pathogens from inoculated beef fillets to non-inoculated ones, through food processing surfaces. three different levels of inoculation of beef fillets surface were prepared: a high one of approximately 10(7) cfu/cm(2), a medium one of 10(5) cfu/cm(2) and a low one of 10(3) cfu/cm(2), using mixed-strains of listeria monocytogenes, or salmonella enterica typhimurium, or escherichia coli o157:h7. the ... | 2017 | 27889165 |
| exposure to minimally processed pear and melon during shelf life could modify the pathogenic potential of listeria monocytogenes. | survival and virulence of foodborne pathogens can be influenced by environmental factors such as the intrinsic properties of food as well as the extrinsic properties that contribute to food shelf life (e.g., temperature and gas atmosphere). the direct contribution of food matrix characteristics on the survival of l. monocytogenes during fresh-cut fruit shelf life is not very well understood. in addition, the gastrointestinal tract is the primary route of listeriosis infection and penetration of ... | 2017 | 27889159 |
| fat and fibre interfere with the dramatic effect that nanoemulsified d-limonene has on the heat resistance of listeria monocytogenes. | the application of d-limonene in form of nanoemulsion has been proved to reduce dramatically the thermal resistance of listeria monocytogenes in culture media. the present research shows very promising results on the application in food products. the thermal resistance of l. monocytogenes was reduced 90 times when 0.5 mm nanoemulsified d-limonene was added to apple juice. this is the biggest reduction in the heat resistance of a microorganism caused by an antimicrobial described ever. however, n ... | 2017 | 27889158 |
| inhibition of listeriolysin o oligomerization by lutein prevents listeria monocytogenes infection. | the foodborne pathogenic bacterial species listeria monocytogenes (l. monocytogenes) has caused incalculable damages to public health, and its successful infection requires various virulence factors, including listeriolysin o (llo). by forming pores in phagosomal membranes and even in some organelles, llo plays an indispensable role in the ability of l. monocytogenes to escape from host immune attacks. because of its critical role, llo offers an appropriate therapeutic target against l. monocyto ... | 2017 | 27884571 |
| antimicrobial activity and bioguided fractionation of rumex tingitanus extracts for meat preservation. | this study was undertaken to investigate the antibacterial and antifungal activities of rumex tingitanus leaves extracts as well as the identification of bioactive components and their performance in meat preservation. total phenolics and flavonoids showed the highest content of phenolics and flavonoids in the ethyl acetate fraction (rt etoacf). for antimicrobial efficacy, leaves extract and derived fraction were tested for their capacity to inhibit bacterial and fungal proliferation in vitro an ... | 2017 | 27883958 |
| immune signatures of protective spleen memory cd8 t cells. | memory cd8 t lymphocyte populations are remarkably heterogeneous and differ in their ability to protect the host. in order to identify the whole range of qualities uniquely associated with protective memory cells we compared the gene expression signatures of two qualities of memory cd8 t cells sharing the same antigenic-specificity: protective (influenza-induced, flu-tm) and non-protective (peptide-induced, tim) spleen memory cd8 t cells. although flu-tm and tim express classical phenotypic memo ... | 2016 | 27883012 |
| closing the brief case: neonatal meningitis caused by listeria monocytogenes diagnosed by multiplex molecular panel. | | 2016 | 27881637 |
| the brief case: neonatal meningitis caused by listeria monocytogenes diagnosed by multiplex molecular panel. | | 2016 | 27881634 |
| how can patients and their physicians contribute to an outbreak investigation? experiences from a nationwide listeriosis outbreak in switzerland. | gathering patient information to contain an outbreak of listeria monocytogenes is difficult because of the patients' severe illness or death. extending the range of interviewees to acquire epidemiological data can thus be important to maximise information. | 2016 | 27878798 |
| proliferation of listeria monocytogenes l-form cells by formation of internal and external vesicles. | l-forms are cell wall-deficient bacteria that divide through unusual mechanisms, involving dynamic perturbations of the cellular shape and generation of vesicles, independently of the cell-division protein ftsz. here we describe ftsz-independent mechanisms, involving internal and external vesicles, by which listeria monocytogenes l-forms proliferate. using micromanipulation of single cells and vesicles, we show that small vesicles are formed by invagination within larger intracellular vesicles, ... | 2016 | 27876798 |
| 1926-2016: 90 years of listeriology. | isopol - for "international symposium on problems of listeria and listeriosis" - meetings gather every three years since 1957 participants from all over the world and allow exchange and update on a wide array of topics concerning listeria and listeriosis, ranging from epidemiology, diagnostic and typing methods, to genomics, post-genomics, fundamental microbiology, cell biology and pathogenesis. the xixth isopol meeting took place in paris from june 14th to 17th, 2016 at institut pasteur. we pro ... | 2016 | 27876526 |
| characteristics of volatile organic compounds produced from five pathogenic bacteria by headspace-solid phase micro-extraction/gas chromatography-mass spectrometry. | the characteristics of volatile compounds from five different bacterial species, escherichia coli o157:h7, salmonella enteritidis, shigella flexneri, staphylococcus aureus, and listeria monocytogenes, growing, respectively, in trypticase soy broth were monitored by headspace solid-phase micro-extraction/gas chromatography-mass spectrometry. the results showed that most volatile organic compounds (vocs) of five pathogens started to increase after the sixth to tenth hour. methyl ketones and long c ... | 2017 | 27874211 |
| inhibitory activity of avocado seed fatty acid derivatives (acetogenins) against listeria monocytogenes. | high standards regarding listeria monocytogenes control and consumer demands for food products without synthetic additives represent a challenge to food industry. we determined the antilisterial properties of an enriched acetogenin extract (eae) from avocado seed, compared it to two commercial antimicrobials (one enriched in avocado acetogenins), and tested purified molecules. acetogenin composition in pulp and seed of hass avocado was quantified. eae were obtained by two sequential centrifuge p ... | 2017 | 27871119 |
| homologous prime-boost vaccination with ova entrapped in self-adjuvanting archaeosomes induces high numbers of ova-specific cd8⁺ t cells that protect against subcutaneous b16-ova melanoma. | homologous prime-boost vaccinations with live vectors typically fail to induce repeated strong cd8⁺ t cell responses due to the induction of anti-vector immunity, highlighting the need for alternative delivery vehicles. the unique ether lipids of archaea may be constituted into liposomes, archaeosomes, which do not induce anti-carrier responses, making them an ideal candidate for use in repeat vaccination systems. herein, we evaluated in mice the maximum threshold of antigen-specific cd8⁺ t cell ... | 2016 | 27869670 |
| infectious dose of listeria monocytogenes in outbreak linked to ice cream, united states, 2015. | the relationship between the number of ingested listeria monocytogenes cells in food and the likelihood of developing listeriosis is not well understood. data from an outbreak of listeriosis linked to milkshakes made from ice cream produced in 1 factory showed that contaminated products were distributed widely to the public without any reported cases, except for 4 cases of severe illness in persons who were highly susceptible. the ingestion of high doses of l. monocytogenes by these patients inf ... | 2016 | 27869595 |
| microbial counts of mealworm larvae (tenebrio molitor) and crickets (acheta domesticus and gryllodes sigillatus) from different rearing companies and different production batches. | the rising interest in insects for human consumption and the changing regulations in europe require a profound insight into the food safety of insects reared and sold in western society. the microbial quality of edible insects has only been studied occasionally. this study aimed at generating an overview of intrinsic parameters (ph, water activity and moisture content) and microbial quality of fresh mealworm larvae and crickets for several rearing companies and for several batches per rearer. in ... | 2017 | 27863329 |
| mesothelin immunotherapy for cancer: ready for prime time? | mesothelin is a tumor antigen that is highly expressed in many human cancers, including malignant mesothelioma and pancreatic, ovarian, and lung adenocarcinomas. it is an attractive target for cancer immunotherapy because its normal expression is limited to mesothelial cells, which are dispensable. several antibody-based therapeutic agents as well as vaccine and t-cell therapies directed at mesothelin are undergoing clinical evaluation. these include antimesothelin immunotoxins (ss1p, rg7787/lmb ... | 2016 | 27863199 |
| emulsion stabilized with starch octenyl succinate prolongs nisin activity against listeria monocytogenes in a cantaloupe juice model. | food safety related to fresh and fresh-cut produce, such as cantaloupe, is a great challenge in the food industry. various processing technologies, including the use of antimicrobial materials, have been applied to reduce pathogens. nisin, an antimicrobial peptide, shows strong inhibitory activity against listeria monocytogenes (lm); however, it suffers from quick depletion in foods. in this study, the protective effect of starch octenyl succinate (starch-os) stabilized emulsion on nisin was eva ... | 2016 | 27861862 |
| lineage ii (serovar 1/2a and 1/2c) human listeria monocytogenes pulsed-field gel electrophoresis types divided into pfge groups using the band patterns below 145.5 kb. | among 504 clinical lineage ii isolates of listeria monocytogenes isolated during 1958-2010 in sweden, 119 pulsed-field gel electrophoresis (pfge) types (asci) have been identified based on the number and distribution of all banding patterns in each dna profile. in this study, these types were further divided into pfge groups based on the configuration of small bands with sizes <145.5 kb. the 504 isolates included 483 serovar 1/2a isolates distributed into 114 pfge types and 21 serovar 1/2c isola ... | 2017 | 27860487 |
| enrichment dynamics of listeria monocytogenes and the associated microbiome from naturally contaminated ice cream linked to a listeriosis outbreak. | microbiota that co-enrich during efforts to recover pathogens from foodborne outbreaks interfere with efficient detection and recovery. here, dynamics of co-enriching microbiota during recovery of listeria monocytogenes from naturally contaminated ice cream samples linked to an outbreak are described for three different initial enrichment formulations used by the food and drug administration (fda), the international organization of standardization (iso), and the united states department of agric ... | 2016 | 27852235 |
| listeria monocytogenes sequence type 1 is predominant in ruminant rhombencephalitis. | listeria (l.) monocytogenes is an opportunistic pathogen causing life-threatening infections in diverse mammalian species including humans and ruminants. as little is known on the link between strains and clinicopathological phenotypes, we studied potential strain-associated virulence and organ tropism in l. monocytogenes isolates from well-defined ruminant cases of clinical infections and the farm environment. the phylogeny of isolates and their virulence-associated genes were analyzed by multi ... | 2016 | 27848981 |
| study of the antibacterial activity of electro-activated solutions of salts of weak organic acids on salmonella enterica, staphylococcus aureus and listeria monocytogenes. | this work assessed the antibacterial activity of electro-activated solutions of salts of weak organic acids (potassium acetate, potassium citrate and calcium lactate) on salmonella enterica, staphylococcus aureus and listeria monocytogenes. this activity was compared in terms of minimal inhibitory (bactericidal) concentration to the effect of commercial acetic, citric and lactic acid at equivalent titratable acidity. staining live/dead baclight method was used to consider physiological state of ... | 2017 | 27838894 |
| listeria monocytogenes-induced cell death inhibits the generation of cell-mediated immunity. | the influence of cell death on adaptive immunity has been studied for decades. despite these efforts, the intricacies of how various cell death pathways shape immune responses in the context of infection remain unclear, particularly with regard to more recently discovered pathways such as pyroptosis. the emergence of listeria monocytogenes as a promising immunotherapeutic platform demands a thorough understanding of how cell death induced in the context of infection influences the generation of ... | 2017 | 27821585 |
| determination of virulence and antibiotic resistance pattern of biofilm producing listeria species isolated from retail raw milk. | one of the foodborne pathogens is listeria monocytogenes, which causes serious invasive illness in elderly and immunocompromised patients, pregnant women, newborns and infants ranking second after salmonellosis because of its high case fatality rate. listerial cow mastitis marked by abnormal milk, increased cell counts and reduced production has not been reported. therefore, apparently healthy cows can be reservoirs of l. monocytogenes. a number of 203 udder milk samples from apparently healthy ... | 2016 | 27821054 |
| structure to function of an α-glucan metabolic pathway that promotes listeria monocytogenes pathogenesis. | here we employ a 'systems structural biology' approach to functionally characterize an unconventional α-glucan metabolic pathway from the food-borne pathogen listeria monocytogenes (lm). crystal structure determination coupled with basic biochemical and biophysical assays allowed for the identification of anabolic, transport, catabolic and regulatory portions of the cycloalternan pathway. these findings provide numerous insights into cycloalternan pathway function and reveal the mechanism of rep ... | 2016 | 27819654 |
| il-37 impairs host resistance to listeria infection by suppressing macrophage function. | listeria monocytogenes is a gram-positive intracellular bacterium that was transmitted through contaminated food and causes sepsis and even death. il-37 has been described as an important anti-inflammatory factor, but little is known about the function of il-37 in host defense against liseria monocytogenes (lm) infection. in mice model of systemic infection, we found that mice treated with il-37 were more sensitive to lm infection compared with pbs-treated mice. this reduced resistance to lm in ... | 2017 | 27816450 |
| short communication: investigation into mycobacterium avium ssp. paratuberculosis in pasteurized milk in italy. | this study investigated the presence of viable mycobacterium avium ssp. paratuberculosis (map) in pasteurized milk produced by italian industrial dairy plants to verify the prediction of a previously performed risk assessment. the study analyzed 160 one-liter bottles of pasteurized milk from 2 dairy plants located in 2 different regions. traditional cultural protocols were applied to 500ml of pasteurized milk for each sample. the investigation focused also on the pasteurization parameters and da ... | 2017 | 27816242 |
| the impact of a cold chain break on the survival of salmonella enterica and listeria monocytogenes on minimally processed 'conference' pears during their shelf life. | in recent years, improved detection methods and increased fresh-cut processing of produce have led to an increased number of outbreaks associated with fresh fruits and vegetables. during fruit and vegetable processing, natural protective barriers are removed and tissues are cut, causing nutrient rich exudates and providing attachment sites for microbes. consequently, fresh-cut produce is more susceptible to microbial proliferation than whole produce. | 2016 | 27813092 |
| prevalence, and virulence determination of listeria monocytogenes strains isolated from clinical and non-clinical samples by multiplex polymerase chain reaction. | this study aimed to determine the prevalence, and virulence factors of listeria monocytogenes isolated from various samples by multiplex polymerase chain reaction (mpcr). | 2016 | 27812659 |
| effectiveness of washing procedures in reducing salmonella enterica and listeria monocytogenes on a raw leafy green vegetable (eruca vesicaria). | vegetables are an important source of nutrients, but they can host a large microbial population, particularly bacteria. foodborne pathogens can contaminate raw vegetables at any stage of their production process with a potential for human infection. appropriate washing can mitigate the risk of foodborne illness consequent to vegetable consumption by reducing pathogen levels, but few data are available to assess the efficacy of different practices. in the present work, six different washing metho ... | 2016 | 27812356 |
| tolerance to quaternary ammonium compound disinfectants may enhance growth of listeria monocytogenes in the food industry. | the antibacterial effect of disinfectants is crucial for the control of listeria monocytogenes in food processing environments. tolerance of l. monocytogenes to sublethal levels of disinfectants based on quaternary ammonium compounds (qac) is conferred by the resistance determinants qach and bcrabc. the presence and distribution of these genes have been anticipated to have a role in the survival and growth of l. monocytogenes in food processing environments where qac based disinfectants are in c ... | 2017 | 27810443 |
| in vivo emergence of rifampicin resistance by rpob mutation in listeria monocytogenes during therapy of prosthetic joint infection. | | 2016 | 27659120 |
| outbreaks and factors influencing microbiological contamination of fresh produce. | fresh fruits and vegetables are nutritionally well-recognised as healthy components in diets. the microbiological foodborne outbreaks associated with the consumption of fresh produce have been increasing. salmonella spp., escherichia coli o157:h7, staphylococcus aureus, campylobacter spp. and listeria monocytogenes are the most common pathogens that contaminate fresh produce. this review discusses recent foodborne outbreaks linked to fresh produce, factors that affect microbiological contaminati ... | 2017 | 27807844 |
| resistance to biocides in listeria monocytogenes collected in meat-processing environments. | the emergence of microorganisms exerting resistance to biocides is a challenge to meat-processing environments. bacteria can be intrinsically resistant to biocides but resistance can also be acquired by adaptation to their sub-lethal concentrations. moreover, the presence of biocide resistance determinants, which is closely linked to antibiotic resistance determinants, could lead to co-selection during disinfection practices along the food chain, and select cross-resistant foodborne pathogens. t ... | 2016 | 27807430 |
| inhibition effect of tea tree oil on listeria monocytogenes growth and exotoxin proteins listeriolysin o and p60 secretion. | listeria monocytogenes (l. monocytogenes) is a gram-positive bacterium that causes infections in humans. in this study, the effects of tea tree oil (tto) at subinhibitory concentrations on l. monocytogenes growth and two important exotoxin proteins secreted by l. monocytogenes were researched. treatment with half of minimal inhibitory concentration of tto demonstrated very little or no reduction in numbers of viable atcc 19115 cells. listeriolysin o (llo) and p60, were investigated. a listerioly ... | 2016 | 27591558 |
| listeria spp. and listeria monocytogenes contamination in ready-to-eat sandwiches collected from vending machines. | ready-to-eat (rte) food is characterised by a long shelf-life at refrigerated temperature and can be consumed as such, without any treatment. the aim of the work was to evaluate the presence of listeria spp. and listeria monocytogenes in rtes collected from refrigerated vending machines placed in hospital environment and accessible to the hospitalised patients. in 4 different sampling, 55 rtes were collected from vending machines of six hospitals located in different areas of sardinia region. al ... | 2016 | 27800439 |
| detection of food hazards in foods: comparison of real time polymerase chain reaction and cultural methods. | foodstuffs should not contain microorganisms or their toxins or metabolites in quantities suggesting an unacceptable risk for human health. the detection of food hazards in foods is performed by several tests that produce results dependent on the analytical method used: an analytical reference method, defined as standard, is associated with each microbiological criterion laid down in regulation 2073/2005/ec, but, analytical methods other than the reference ones, in particular more rapid methods, ... | 2016 | 27800434 |
| antimicrobial activity of chitosan films with essential oils against listeria monocytogenes on cabbage. | the highest incidence of listeriosis, due to consumption of ready-to-eat foods and fresh, shredded, minimally processed vegetables, occurs among pregnant women and the elderly. in order to reduce the prevalence of listeriosis among consumers, better protective measures are recommended. chitosan films, with or without added essential oils, represent a modern, safe method of preserving the quality of such vegetables and significantly reducing the incidence of listeria monocytogenes in these foods. | 2016 | 27800143 |
| adaptive response of listeria monocytogenes to heat, salinity and low ph, after habituation on cherry tomatoes and lettuce leaves. | pathogens found on fresh produce may encounter low temperatures, high acidity and limited nutrient availability. the aim of this study was to evaluate the effect of habituation of listeria monocytogenes on cherry tomatoes or lettuce leaves on its subsequent response to inhibitory levels of acid, osmotic and heat stress. habituation was performed by inoculating lettuce coupons, whole cherry tomatoes or tryptic soy broth (tsb) with a three-strains composite of l. monocytogenes, which were further ... | 2016 | 27798688 |
| menin plays a critical role in the regulation of the antigen-specific cd8+ t cell response upon listeria infection. | menin, a tumor suppressor protein, is encoded by the men1 gene in humans. certain germinal mutations of men1 induce an autosomal-dominant syndrome that is characterized by concurrent parathyroid adenomas and several other tumor types. although menin is also expressed in hematopoietic lineages, its role in cd8(+) t cells remains unclear. we generated menin(flox/flox) cd4-cre (menin-ko) mice by crossing menin(flox/flox) mice with cd4-cre transgenic (tg) mice to determine the role of menin in cd8(+ ... | 2016 | 27798149 |
| suppressor mutations linking gpsb with the first committed step of peptidoglycan biosynthesis in listeria monocytogenes. | the cell division protein gpsb is a regulator of the penicillin binding protein a1 (pbp a1) in the gram-positive human pathogen listeria monocytogenes penicillin binding proteins mediate the last two steps of peptidoglycan biosynthesis as they polymerize and cross-link peptidoglycan strands, the main components of the bacterial cell wall. it is not known what other processes are controlled by gpsb. l. monocytogenes gpsb mutants are unable to grow at 42°c, but we observed that spontaneous suppres ... | 2017 | 27795316 |
| inlp, a new virulence factor with strong placental tropism. | intrauterine infection is a major detriment for maternal-child health and occurs despite local mechanisms that protect the maternal-fetal interface from a wide variety of pathogens. the bacterial pathogen listeria monocytogenes causes spontaneous abortion, stillbirth, and preterm labor in humans and serves as a model for placental pathogenesis. given the unique immunological environment of the maternal-fetal interface, we hypothesized that virulence determinants with placental tropism are requir ... | 2016 | 27736782 |
| listeria monocytogenes encodes a functional esx-1 secretion system whose expression is detrimental to in vivo infection. | bacterial pathogenicity deeply depends on the ability to secrete virulence factors that bind specific targets on host cells and manipulate host responses. the gram-positive bacterium listeria monocytogenes is a human foodborne pathogen that remains a serious public health concern. to transport proteins across its cell envelope, this facultative intracellular pathogen engages a set of specialized secretion systems. here we show that l. monocytogenes egde uses a specialized secretion system, named ... | 2016 | 27723420 |
| senp1 regulates ifn-γ-stat1 signaling through stat3-socs3 negative feedback loop. | interferon-γ (ifn-γ) triggers macrophage for inflammation response by activating the intracellular jak-stat1 signaling. suppressor of cytokine signaling 1 (socs1) and protein tyrosine phosphatases can negatively modulate ifn-γ signaling. here, we identify a novel negative feedback loop mediated by stat3-socs3, which is tightly controlled by senp1 via de-sumoylation of protein tyrosine phosphatase 1b (ptp1b), in ifn-γ signaling. senp1-deficient macrophages show defects in ifn-γ signaling and m1 m ... | 2016 | 27702761 |
| listeria infections of the eye. | the bacterium listeria monocytogenes resides originally in the environment. infections of the eye have been induced experimentally; for example, in rabbits and guinea pigs. natural ocular infections occur in various animals; in most instances, they are induced exogenously; for example, by contaminated silage affecting primarily the conjunctiva, cornea, or the anterior chamber. sporadic infections as well as outbreaks have been described. in humans, besides exogenous infections, endogenous infect ... | 2017 | 27791252 |
| met-activating residues in the b-repeat of the listeria monocytogenes invasion protein inlb. | the facultative intracellular pathogen listeria monocytogenes causes listeriosis, a rare but life-threatening disease. host cell entry begins with activation of the human receptor tyrosine kinase met through the bacterial invasion protein inlb, which contains an internalin domain, a b-repeat, and three gw domains. the internalin domain is known to bind met, but no interaction partner is known for the b-repeat. adding the b-repeat to the internalin domain potentiates met activation and is require ... | 2016 | 27789707 |
| expression of surface protein lapb by a wide spectrum of listeria monocytogenes serotypes as demonstrated with anti-lapb monoclonal antibodies. | protein antigens expressed on the surface of all strains of listeria monocytogenes and absent from nonpathogenic listeria spp. are presumably useful targets for pathogen identification, detection, and isolation using specific antibodies (abs). to seek such surface proteins expressed in various strains of l. monocytogenes for diagnostic applications, we focused on a set of surface proteins known to be involved or putatively involved in l. monocytogenes virulence and identified listeria adhesion p ... | 2016 | 27613687 |
| antimicrobial resistance of listeria monocytogenes isolated from seafood and humans in iran. | fourteen listeria monocytogenes isolates previously collected from seafood (n = 7) and human patients (n = 7) were studied for their antimicrobial susceptibility against eight common antimicrobials (ampicillin, penicillin, gentamicin, streptomycin, tetracycline, trimethoprim-sulfamethoxazole, chloramphenicol, and cefotaxime). a high resistance level to ampicillin, cefotaxime (100%), and pencillin (57% in seafood isolates and 71.4% in clinical isolates) was observed in this study. however, all of ... | 2016 | 27622345 |
| 8-thioalkyl-adenosine derivatives inhibit listeria monocytogenes nad kinase through a novel binding mode. | increased resistance of pathogens to existing antibiotics necessitates the search for novel targets to develop potent antimicrobials. biosynthetic pathways of several cofactors important for bacterial growth, such as nicotinamide adenine dinucleotide phosphate (nadp), have been proposed as a promising source of antibiotic targets. nicotinamide adenine dinucleotide kinases (nadk; ec 2.7.1.23) are attractive for inhibitor development, since they catalyze the phosphorylation of nad to nadp, which i ... | 2016 | 27783975 |
| survival and metabolic activity of pediocin producer pediococcus acidilactici ul5: its impact on intestinal microbiota and listeria monocytogenes in a model of the human terminal ileum. | pediococcus acidilactici ul5 is a promising probiotic candidate due to its high survival rate under gastric and duodenal conditions and to its ability to produce the antilisterial bacteriocin pediocin pa-1. its survival, metabolic activity, and impact on listeria monocytogenes in a continuous stirred tank reactor containing immobilized human intestinal microbiota were studied over a period of 32 days of feeding a nutrient medium simulating ileal chyme. the impact of p. acidilactici ul5 on differ ... | 2016 | 26162534 |
| transport and catabolism of pentitols by listeria monocytogenes. | transposon insertion into listeria monocytogenes lmo2665, which encodes an eiic of the phosphoenolpyruvate:carbohydrate phosphotransferase system (pts), was found to prevent d-arabitol utilization. we confirm this result with a deletion mutant and show that lmo2665 is also required for d-xylitol utilization. we therefore called this protein eiicaxl. both pentitols are probably catabolized via the pentose phosphate pathway (ppp) because lmo2665 belongs to an operon, which encodes the three ptsaxl ... | 2016 | 27553222 |
| innate and adaptive immunologic functions of complement in the host response to listeria monocytogenes infection. | listeria monocytogenes is a leading cause of foodborne-illness associated mortality that has attracted considerable attention in recent years due to several significant outbreaks. it has also served as a model organism for the study of intracellular pathogens. for these reasons the host response to l. monocytogenes has long been the subject of investigation. a potent innate and adaptive immune response is required for containment and clearance of l. monocytogenes. however, some elements of this ... | 2016 | 27476791 |
| rickettsia sca4 reduces vinculin-mediated intercellular tension to promote spread. | spotted fever group (sfg) rickettsiae are human pathogens that infect cells in the vasculature. they disseminate through host tissues by a process of cell-to-cell spread that involves protrusion formation, engulfment, and vacuolar escape. other bacterial pathogens rely on actin-based motility to provide a physical force for spread. here, we show that sfg species rickettsia parkeri typically lack actin tails during spread and instead manipulate host intercellular tension and mechanotransduction t ... | 2016 | 27768890 |
| induction and stability of oxidative stress adaptation in listeria monocytogenes egd (bug600) and f1057 in sublethal concentrations of h2o2 and naoh. | food processing and food handling environments may contain residual levels of sanitizers or cleaners which may trigger oxidative stress adaptation in listeria monocytogenes. the aim of this study was to determine the induction and stability of oxidative stress adaptation in l. monocytogenes egd (bug600) (serotype 1/2a) and f1057 (serotype 4b) at different concentrations and times of sublethal oxidative stress induced by h2o2 or sublethal alkali stress induced by naoh at 37°c. both l. monocytogen ... | 2016 | 27716471 |
| applicability of the en iso 11290-1 standard method for listeria monocytogenes detection in presence of new listeria species. | during the past six years, new species of the genus listeria have been isolated from foods and other environmental niches worldwide. the standard method en iso 11290-1 that is currently under revision will include in its scope all listeria species in addition to l. monocytogenes. the objective of this project was to evaluate the ability of the standard en iso 11290-1 method to detect and identify the newly discovered listeria spp., and to assess potential over-growth effects of the new species i ... | 2016 | 27716470 |
| effect of sodium alginate coating incorporated with nisin, cinnamomum zeylanicum, and rosemary essential oils on microbial quality of chicken meat and fate of listeria monocytogenes during refrigeration. | the present study was conducted to preserve the microbial quality of chicken meat fillets during storage time by using sodium alginate active coating solutions incorporated with different natural antimicrobials including nisin, cinnamomum zeylanicum (cinnamon), and rosemary essential oils (eos) which were added individually and in combination. the samples were stored in refrigeration condition for 15days and were analyzed for total viable count, enterobacteriaceae count, lactic acid bacteria cou ... | 2016 | 27620825 |
| characterization of a plasmid carrying cat, ermb and tets genes in a foodborne listeria monocytogenes strain and uptake of the plasmid by cariogenic streptococcus mutans. | a multi-drug resistant (mdr) listeria monocytogenes isolate (serotype 1/2c) was recovered from a quick-frozen rice flour product collected from langfang city in northern china. pcr screening identified the presence of cat, ermb and tets genes. the plasmid profile of the strain showed the presence of an approximately 22.4-kb plasmid. curing of this plasmid resulted in the loss of cat, ermb and tets genes and increased susceptibility to several antibiotics, suggesting the involvement of the plasmi ... | 2016 | 27592072 |
| survival of foodborne pathogens (escherichia coli o157:h7, salmonella typhimurium, staphylococcus aureus, listeria monocytogenes, and vibrio parahaemolyticus) in raw ready-to-eat crab marinated in soy sauce. | knowing the survival characteristics of foodborne pathogens in raw ready-to-eat (rte) seafood is the key to predicting whether they pose a microbiological hazard. the present study examined the survival of escherichia coli o157:h7, salmonella typhimurium, vibrio parahaemoliticus, listeria monocytogenes, and staphylococcus aureus in raw rte crab marinated in soy sauce. inoculated crabs (initial bacterial population=4.1-4.4logcfu/g) were immersed in soy sauce and then stored at refrigeration (5°c) ... | 2016 | 27591386 |
| development of synthetic media mimicking food soils to study the behaviour of listeria monocytogenes on stainless steel surfaces. | listeria monocytogenes is one of the main targets of hygiene procedures in the ready-to-eat food industry due to its ability to persist for months or even years in processing plants, where it can contaminate food during processing. the factors associated with persistence are often those that foster growth, which itself depends on food contamination of surfaces. it is therefore essential to experiment by using food soils or media modelling these soils to understand the behaviour of l. monocytogen ... | 2016 | 27589019 |
| interferon regulatory factor 4 controls th1 cell effector function and metabolism. | the transcription factor interferon regulatory factor 4 (irf4) is essential for th2 and th17 cell formation and controls peripheral cd8(+) t cell differentiation. we used listeria monocytogenes infection to characterize the function of irf4 in th1 responses. irf4(-/-) mice generated only marginal numbers of listeria-specific th1 cells. after transfer into infected mice, irf4(-/-) cd4(+) t cells failed to differentiate into th1 cells as indicated by reduced t-bet and ifn-γ expression, and showed ... | 2016 | 27762344 |
| data on the effect of cytopiloyne against listeria monocytogenes infection in mice. | cytopiloyne (cp), a novel polyacetylene compound extracted from b. pilosa, shows a multi-bioactivity, including immunomodulatory and antidiabetes. here, we investigated the anti-listeria effect of cytopiloyne in mice by assessing mortality, clearance of l. monocytogenes, and pathology examination. the data presented herein are supplemental to our research article entitled "cytopiloyne, a polyacetylenic glucoside from bidens pilosa, acts as a novel anticandidial agent via regulation of macrophage ... | 2016 | 27761492 |
| hydrogen peroxide-mediated conversion of coproheme to heme b by hemq-lessons from the first crystal structure and kinetic studies. | heme biosynthesis in gram-positive bacteria follows a recently described coproporphyrin-dependent pathway with hemq catalyzing the decarboxylation of coproheme to heme b. here we present the first crystal structure of a hemq (homopentameric coproheme-hemq from listeria monocytogenes) at 1.69 å resolution and the conversion of coproheme to heme b followed by uv-vis and resonance raman spectroscopy as well as mass spectrometry. the ferric five-coordinated coproheme iron of hemq is weakly bound by ... | 2016 | 27758026 |
| anti-listeria effects of chitosan-coated nisin-silica liposome on cheddar cheese. | listeria monocytogenes poses an increasing challenge to cheese production. to minimize the risk of bacterial contamination, a chitosan-coated nisin-silica liposome was engineered for the present study. we investigated the characteristics of nisin-silica liposomes and the anti-listeria effects of a chitosan-coated nisin-silica liposome on cheddar cheese. the encapsulation efficiency of nisin in a liposome was sharply increased after it was adsorbed on a silica particle surface. chitosan-coated ni ... | 2016 | 27568051 |
| paper-based bipolar electrode electrochemiluminescence switch for label-free and sensitive genetic detection of pathogenic bacteria. | genetic analysis is of great importance for the detection of pathogenic bacteria. bacterial identification must become simpler, less expensive, and more rapid than the traditional methods. in this study, a low-cost, label-free, and wireless paper-based bipolar electrode electrochemiluminescence (pbpe-ecl) analysis system was constructed for the rapid and sensitive genetic detection of pathogenic bacteria. wax-screen printing was used to form hydrophilic channels on filter paper, and a carbon ink ... | 2016 | 27633711 |
| comparative evaluation of direct plating and most probable number for enumeration of low levels of listeria monocytogenes in naturally contaminated ice cream products. | a precise and accurate method for enumeration of low level of listeria monocytogenes in foods is critical to a variety of studies. in this study, paired comparison of most probable number (mpn) and direct plating enumeration of l. monocytogenes was conducted on a total of 1730 outbreak-associated ice cream samples that were naturally contaminated with low level of l. monocytogenes. mpn was performed on all 1730 samples. direct plating was performed on all samples using the rapid'l.mono (rlm) aga ... | 2017 | 27741432 |
| effects of low nano2 and nacl concentrations on listeria monocytogenes growth in emulsion-type sausage. | the objective of this study was to evaluate the effect of combinations of nano2 and nacl concentrations on listeria monocytogenes (l. monocytogenes) growth in emulsion-type sausage. | 2017 | 27739291 |
| alteration of epithelial cell lysosomal integrity induced by bacterial cholesterol-dependent cytolysins. | bacterial pathogens can interfere during infection with host cell organelles, such as mitochondria, the endoplasmic reticulum-golgi system or nuclei. as important cellular functions are often compartmentalized in these organelles, their targeting allows pathogens to manipulate key host functions during infection. here, we identify lysosomes as a new class of organelles targeted by the pathogenic bacterium listeria monocytogenes. we demonstrate that extracellular listeria, via secretion of the po ... | 2017 | 27739224 |
| listeria monocytogenes infection of hd11, chicken macrophage-like cells. | listeria monocytogenes can be carried by and infect poultry, although the clinical disease in birds is rare. escape from macrophage phagocytosis is a key step in pathogenesis for l. monocytogenes therefore, we investigated the infection of the chicken macrophage-like cell line hd11 with 2 strains of l. monocytogenes egd-e and scott a. after infection, l. monocytogenes was quantified by spread plating and hd11 was quantified with trypan blue exclusion stain before enumeration. the standard macrop ... | 2016 | 27738121 |
| genome sequence of listeria monocytogenes plasmid plm-c-273 carrying genes related to stress resistance. | mobile genetic elements in bacteria, such as plasmids, act as important vectors for the transfer of antibiotic resistance, virulence, and metal resistance genes. here, we report the genome sequence of a new plasmid plm-c-273, identified in a listeria monocytogenes strain isolated from a clinical sample in ontario, canada. | 2016 | 27738039 |
| epitope-specific vaccination limits clonal expansion of heterologous naive t cells during viral challenge. | despite robust secondary t cell expansion primed by vaccination, the impact on primary immune responses to heterotypic antigens remains undefined. here we show that secondary expansion of epitope-specific memory cd8(+) t cells primed by prior infection with recombinant pathogens limits the primary expansion of naive cd8(+) t cells with specificity to new heterologous antigens, dampening protective immunity against subsequent pathogen challenge. the degree of naive t cell repression directly para ... | 2016 | 27732841 |
| the development of methods for the detection of salmonella in chickens by a combination of immunomagnetic separation and pcrs. | micro- and nanoimmunomagnetic beads (mimbs and nimbs) used for immunomagnetic separation (ims) with pcr were studied for the rapid detection of salmonella. the capture efficiency of the two different imbs was evaluated by a conventional plate counting method, and the binding pattern was studied using scanning electron microscopy. the specificity of the imbs was tested with salmonella, shigella flexneri, enterohemorrhagic escherichia coli o157:h7, and listeria monocytogenes. by comparing the pre- ... | 2016 | 27730684 |
| acta of listeria monocytogenes and its manifold activities as an important listerial virulence factor. | listeria monocytogenes is a ubiquitously occurring gram-positive bacterium in the environment that causes listeriosis, one of the deadliest foodborne infections known today. it is a versatile facultative intracellular pathogen capable of growth within the host's cytosolic compartment. following entry into the host cell, l. monocytogenes escapes from vacuolar compartments to the cytosol, where the bacterium begins a remarkable journey within the host cytoplasm, culminating in bacterial spread fro ... | 2017 | 27726006 |
| genetic subtyping of listeria monocytogenes via multiple-locus sequence typing using iap, sigb and acta. | pulse field gel electrophoresis (pfge) is widely used for listeriosis surveillance. although this technique is effective for epidemiology, the data among laboratories are inconsistent. we previously reported a method for listeria monocytogenes subtyping combined with sequence analysis of partial iap and whole genome restriction fragment length polymorphism (rflp) using xbai, clai (baniii) and psti. however, distinguishing subtypes was challenging, because the output comprised complicated fragmen ... | 2017 | 27725353 |
| diversity of listeria monocytogenes strains of clinical and food chain origins in belgium between 1985 and 2014. | listeriosis is a rare but severe disease, mainly caused by listeria monocytogenes. this study shows the results of the laboratory-based surveillance of listeriosis in belgium over the period 1985-2014. besides the incidence and some demographic data we present also more detailed microbiological and molecular characteristics of human strains isolated since 2000. the strains from the latter period were compared to food and animal strains from the same period. our study shows that different food ma ... | 2016 | 27723768 |