| a 3-year multi-food study of the presence and persistence of listeria monocytogenes in 54 small food businesses in ireland. | the problem of assessing the occurrence of the food-borne pathogen listeria monocytogenes in the food chain, and therefore the risk of exposure of the human population, is often challenging because of the limited scope of some studies. in this study the occurrence of l. monocytogenes in food from four major food groups, dairy products, meats, seafood and vegetables, and associated food processing environments in ireland was studied over a three-year period. fifty-four small food businesses parti ... | 2017 | 28271853 |
| structural insights into glutathione-mediated activation of the master regulator prfa in listeria monocytogenes. | | 2017 | 28271443 |
| listeria monocytogenes induces an interferon-enhanced activation of the integrated stress response that is detrimental for resolution of infection in mice. | type i interferons (ifns) induce a detrimental response during listeria monocytogenes (l. monocytogenes) infection. we were interested in identifying mechanisms linking ifn signaling to negative host responses against l. monocytogenes infection. herein we found that infection of myeloid cells with l. monocytogenes led to a coordinated induction of type i ifns and activation of the integrated stress response (isr). infected cells did not induce xbp1 splicing or bip upregulation, indicating that t ... | 2017 | 28267207 |
| the position of prenylation of isoflavonoids and stilbenoids from legumes (fabaceae) modulates the antimicrobial activity against gram positive pathogens. | the legume plant family (fabaceae) is a potential source of antimicrobial phytochemicals. molecular diversity in phytochemicals of legume extracts was enhanced by germination and fungal elicitation of seven legume species, as established by rp-uhplc-uv-ms. the relationship between phytochemical composition, including different types of skeletons and substitutions, and antibacterial properties of extracts was investigated. extracts rich in prenylated isoflavonoids and stilbenoids showed potent an ... | 2017 | 28254012 |
| microbial survey of ready-to-eat salad ingredients sold at retail reveals the occurrence and the persistence of listeria monocytogenes sequence types 2 and 87 in pre-packed smoked salmon. | as the preparation of salads involves extensive handling and the use of uncooked ingredients, they are particularly vulnerable to microbial contamination. this study aimed to determine the microbial safety and quality of pre-packed salads and salad bar ingredients sold in singapore, so as to identify public health risks that could arise from consuming salads and to determine areas for improvement in the management of food safety. | 2017 | 28245788 |
| apoptotic cells enhance pathogenesis of listeria monocytogenes. | infections by pathogenic microorganisms elicit host immune responses, which crucially limit those infections. pathogens employ various strategies to evade host immunity. we have identified the exploitation of the repertoire of potent immunosuppressive responses elicited normally by apoptotic cells ("innate apoptotic immunity"; iai) as one of these strategies. in the case of listeria monocytogenes, an environmentally ubiquitous, foodborne bacterial pathogen capable of causing life-threatening inv ... | 2017 | 28242425 |
| adenovirus serotype 5 vaccine vectors trigger il-27-dependent inhibitory cd4(+) t cell responses that impair cd8(+) t cell function. | adenovirus serotype 5 (ad5) vaccine vectors elicit robust cd8(+) t cell responses, but these responses typically exhibit a partially exhausted phenotype. however, the immunologic mechanism by which ad5 vectors induce dysfunctional cd8(+) t cells has not previously been elucidated. here we demonstrate that, following immunization of b6 mice, ad5 vectors elicit antigen-specific il-10(+)cd4(+) t cells with a distinct transcriptional profile in a dose-dependent fashion. in rhesus monkeys, we similar ... | 2016 | 28239679 |
| growth, viability and architecture of biofilms of listeria monocytogenes formed on abiotic surfaces. | the pathogenic bacterium listeria monocytogenes can persist in food processing plants for many years, even when appropriate hygienic measures are in place, with potential for contaminating ready-to-eat products and, its ability to form biofilms on abiotic surfaces certainly contributes for the environmental persistence. in this research, l. monocytogenes was grown in biofilms up 8 days attached to stainless steel and glass surfaces, contributing for advancing the knowledge on architecture of mat ... | 2017 | 28237677 |
| an evaluation of alternatives to nitrites and sulfites to inhibit the growth of salmonella enterica and listeria monocytogenes in meat products. | in recent years, the use of nitrites and sulfites as food preservatives has been a cause for concern due to the health problems that these additives can cause in humans. natural products have been studied as an alternative, but most of them have hardly been applied in the food industry for technological and economic reasons. in this sense, organic salts such as sodium acetate are a good alternative due to their affordability. thus, this study evaluated the capacity of sodium nitrite, sodium sulf ... | 2016 | 28231169 |
| behavior of salmonella and listeria monocytogenes in raw yellowfin tuna during cold storage. | behavior of salmonella and listeria monocytogenes in raw yellowfin tuna during refrigeration and frozen storage were studied. growth of salmonella was inhibited in tuna during refrigerated storage, while l. monocytogenes was able to multiply significantly during refrigerated storage. populations of salmonella in tuna were reduced by 1 to 2 log after 12 days of storage at 5-7 °c, regardless levels of contamination. however, populations of l. monocytogenes scott a, m0507, and sfl0404 in inoculated ... | 2016 | 28231111 |
| acute fetal demise with first trimester maternal infection resulting from listeria monocytogenes in a nonhuman primate model. | infection with listeria monocytogenes during pregnancy is associated with miscarriage, preterm birth, and neonatal complications, including sepsis and meningitis. while the risk of these conditions is thought to be greatest during the third trimester of pregnancy, the determinants of fetoplacental susceptibility to infection, the contribution of gestational age, and the in vivo progression of disease at the maternal-fetal interface are poorly understood. we developed a nonhuman primate model of ... | 2017 | 28223455 |
| listeria monocytogenes in smoked salmon and other smoked fish at retail in italy: frequency of contamination and strain characterization in products from different manufacturers. | seven hundred seventy-eight samples of packaged smoked fish (774 smoked salmon and 4 smoked swordfish) on sale in italy, from 50 different manufacturers located in 12 european union countries, were purchased from the italian market between may and december 2011. the surface temperatures of the samples on sale ranged from 0 to 13°c (3.4 ± 1.5°c, mean ± sd). six hundred eighty (87.4%) of 778 samples were stored at ≤4°c. one hundred fifty-seven samples (20.2%, 95% confidence interval 17.5 to 23.1%) ... | 2017 | 28221976 |
| fate of listeria monocytogenes , pathogenic yersinia enterocolitica , and escherichia coli o157:h7 gfp(+) in ready-to-eat salad during cold storage: what is the risk to consumers? | in this study, we investigated the fate of listeria monocytogenes , pathogenic yersinia enterocolitica , and escherichia coli o157:h7 gfp(+) inoculated in low numbers into ready-to-eat baby spinach and mixed-ingredient salad (baby spinach with chicken meat). samples were stored at recommended maximum refrigerator temperature (8°c in sweden) or at an abuse temperature (15°c) for up to 7 days. mixed-ingredient salad supported considerable growth when stored at 15°c during shelf life (3 days), with ... | 2017 | 28221975 |
| development and validation of pathogen environmental monitoring programs for small cheese processing facilities. | pathogen environmental monitoring programs (emps) are essential for food processing facilities of all sizes that produce ready-to-eat food products exposed to the processing environment. we developed, implemented, and evaluated emps targeting listeria spp. and salmonella in nine small cheese processing facilities, including seven farmstead facilities. individual emps with monthly sample collection protocols were designed specifically for each facility. salmonella was detected in only one facilit ... | 2016 | 28221969 |
| synergistic antilisterial effects of mixtures of lysozyme and organic acids. | we investigated the synergistic effects of lysozyme combined with organic acids to inhibit the growth of listeria monocytogenes . the antilisterial effects of the combination of lysozyme and acetic acid, citric acid, lactic acid, malic acid, or succinic acid were evaluated using the checkerboard method and time-kill assay. the mic was 25,000 mg/liter for lysozyme, 625 mg/liter for acetic acid, and 1,250 mg/liter for the other acids. the mbc was 10,000 mg/liter for all of the tested organic acids ... | 2016 | 28221961 |
| effect of power levels on inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in tomato paste using 915-megahertz microwave and ohmic heating. | the effect of power levels on inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in tomato paste was investigated using 915-mhz microwave heating (mw) and ohmic heating (oh). heating uniformity, pathogen inactivation, and quality aspects were determined with 1.8-, 2.1-, 2.4-, and 3.0-kw mw and corresponding oh. ginafit was used to analyze pathogen inactivation. the heating uniformity of mw-treated samples was inferior to that of oh-treated samples at low ... | 2016 | 28221946 |
| effect of temperature and nutrient concentration on survival of foodborne pathogens in deciduous fruit processing environments for effective hygiene management. | temperature and good sanitation practices are important factors for controlling growth of microorganisms. fresh produce is stored at various temperatures to ensure quality and to prolong shelf life. when foodborne pathogens survive and grow on fresh produce at storage temperatures, then additional control strategies are needed to inactivate these pathogens. the aim of this study was to determine how temperatures associated with deciduous fruit processing and storage facilities (0.5, 4, and 21°c) ... | 2016 | 28221909 |
| prevalence and level of listeria monocytogenes in ice cream linked to a listeriosis outbreak in the united states. | a most-probable-number (mpn) method was used to enumerate listeria monocytogenes in 2,320 commercial ice cream scoops manufactured on a production line that was implicated in a 2015 listeriosis outbreak in the united states. the analyzed samples were collected from seven lots produced in november 2014, december 2014, january 2015, and march 2015. l. monocytogenes was detected in 99% (2,307 of 2,320) of the tested samples (lower limit of detection, 0.03 mpn/g), 92% of which were contaminated at < ... | 2016 | 28221903 |
| prevalence and persistence of listeria monocytogenes in ready-to-eat tilapia sashimi processing plants. | a 2-year study was performed at two ready-to-eat tilapia sashimi processing plants (a and b) to identify possible routes of contamination with listeria monocytogenes during processing. samples were collected from the aquaculture environments, transportation tanks, processing plants, and final products. seventy-nine l. monocytogenes isolates were found in the processing environments and final products; 3.96% (50 of 1,264 samples) and 3.86% (29 of 752 samples) of the samples from plants a and b, r ... | 2016 | 28221901 |
| microbial quality, safety, and pathogen detection by using quantitative pcr of raw salad vegetables sold in dhanbad city, india. | consumption of ready-to-eat fresh vegetables has increased worldwide, with a consequent increase in outbreaks caused by foodborne pathogens. in the indian subcontinent, raw fresh vegetables are usually consumed without washing or other decontamination procedures, thereby leading to new food safety threats. in this study, the microbiological quality and pathogenic profile of raw salad vegetables was evaluated through standard protocols. in total, 480 samples (60 each of eight different salad vege ... | 2017 | 28221891 |
| prediction of escherichia coli o157:h7, salmonella , and listeria monocytogenes growth in leafy greens without temperature control. | a recent study by the centers for disease control and prevention reported that between 1998 and 2008, leafy greens outbreaks accounted for 22.3% of foodborne outbreaks in the united states. several studies on the growth of bacteria at different temperatures have been conducted; however, there is a need for the prediction of bacterial growth when leafy greens are transported without temperature control. food products, when taken out of refrigeration, undergo a temperature change, with the rate of ... | 2017 | 28221878 |
| listeria monocytogenes isolates carrying virulence-attenuating mutations in internalin a are commonly isolated from ready-to-eat food processing plant and retail environments. | listeria monocytogenes is a human foodborne pathogen that may cause an invasive disease known as listeriosis in susceptible individuals. internalin a (inla; encoded by inla) is a virulence factor that facilitates crossing of host cell barriers by l. monocytogenes . at least 19 single nucleotide polymorphisms (snps) in inla that result in a premature stop codon (pmsc) have been described worldwide. snps leading to a pmsc in inla have been shown to be causally associated with attenuated virulence. ... | 2016 | 28221857 |
| fate of pathogenic bacteria associated with production of pickled sausage by using a cold fill process. | preservation by pickling has been used for many years to extend the shelf life of various types of food products. by storing meat products in a brine solution containing an organic acid, salt, spices, as well as other preservatives, the ph of the product is reduced, thus increasing the safety and shelf life of the product. pickling may involve the use of heated brines to further add to the safety of the food product. when precooked, ready-to-eat (rte) sausages are pickled with a heated brine sol ... | 2016 | 28221846 |
| environmental occurrence and antibiotic susceptibility profile of listeria monocytogenes at a slaughterhouse raw processing plant in romania. | this survey was conducted to investigate the occurrence and antimicrobial susceptibility of listeria monocytogenes isolates in the environment of a pig slaughterhouse raw processing plant complex in western romania. a total of 97 environmental samples from food contact (n = 60) and nonfood contact (n = 37) surfaces were examined with standard methods. the susceptibility of the isolates to 17 antimicrobial agents was determined with the vitek 2 automated system. overall, 25 (25.8%) samples were p ... | 2016 | 28221837 |
| listeria monocytogenes 10403s arginine repressor argr finely tunes arginine metabolism regulation under acidic conditions. | listeria monocytogenes is able to colonize human and animal intestinal tracts and to subsequently cross the intestinal barrier, causing systemic infection. for successful establishment of infection, l. monocytogenes must survive the low ph environment of the stomach. l. monocytogenes encodes a functional argr, a transcriptional regulator belonging to the argr/ahrc arginine repressor family. we aimed at clarifying the specific functions of argr in arginine metabolism regulation, and more importan ... | 2017 | 28217122 |
| monocytes are the predominant cell type associated with listeria monocytogenes in the gut, but they do not serve as an intracellular growth niche. | after foodborne transmission of the facultative intracellular bacterial pathogen listeria monocytogenes, most of the bacterial burden in the gut is extracellular. however, we previously demonstrated that intracellular replication in an as yet unidentified cell type was essential for dissemination and systemic spread of l. monocytogenes in this article, we show that the vast majority of cell-associated l. monocytogenes in the gut were adhered to ly6c(hi) monocytes, a cell type that inefficiently ... | 2017 | 28213502 |
| high hydrostatic pressure effects on listeria monocytogenes and l. innocua: evidence for variability in inactivation behaviour and in resistance to pediocin bacha-6111-2. | the effect of high hydrostatic pressure (hhp) on the survival of 14 strains of listeria monocytogenes from food or clinical origins, selected to represent different pheno and genotypes, was evaluated. stationary phase cells were submitted to 300, 400 and 500 mpa at 10 °c, for 5 min. a high variability in the resistance of l. monocytogenes to pressure was observed, and particularly two strains isolated from food were significantly more baroresistant than the rest. strains of l. monocytogenes resi ... | 2017 | 28213030 |
| inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in ready-to-bake cookie dough by gamma and electron beam irradiation. | this study was conducted to investigate the efficacy of gamma and electron beam irradiation to inactivate foodborne pathogens in ready-to-bake cookie dough and to determine the effect on quality by measuring color and texture changes. cookie dough inoculated with escherichia coli o157:h7, salmonella typhimurium, or listeria monocytogenes was subjected to gamma and electron beam irradiation, with doses ranging from 0 to 3 kgy. as the radiation dose increased, the inactivation effect increased amo ... | 2017 | 28213023 |
| prevalence, genetic diversity and antimicrobial resistance of listeria monocytogenes isolated from fresh and smoked fish in poland. | a total of 57 out of 301 (18.9%) fresh and smoked fish samples in poland were positive for listeria monocytotgenes. the bacteria were most frequently identified in fresh and smoked salmon (32.0% and 33.8% respectively) as well as in fresh cod (31.8%). only three samples of smoked salmon were contaminated with the bacteria above 100 cfu/g. four molecular serogroups were identified and the most prevalent, 1/2a-3a (40 isolates; 70.2%), was present in samples from both marine (33 strains; 71.7%) and ... | 2017 | 28213022 |
| pathogen biofilm formation on cantaloupe surface and its impact on the antibacterial effect of lauroyl arginate ethyl. | pathogen biofilm at fruit surface may pose a particular risk to food safety. in this study, the biofilms of listeria monocytogenes v7 and salmonella enterica serovar typhimurium atcc 13311 on cantaloupe fruit surface were visualized, and the resistance of biofilms against lauroyl arginate ethyl (lae, an antibacterial compound) was evaluated. each bacterium was inoculated on isolated cantaloupe rind surfaces at 10(5)-10(6) cfu/cm(2) and after incubation for 2, 12, 24, and 48 h, the surfaces were ... | 2017 | 28213018 |
| assessment of the antimicrobial activity of olive leaf extract against foodborne bacterial pathogens. | olive leaf extract (ole) has been used traditionally as a herbal supplement since it contains polyphenolic compounds with beneficial properties ranging from increasing energy levels, lowering blood pressure, and supporting the cardiovascular and immune systems. in addition to the beneficial effects on human health, ole also has antimicrobial properties. the aim of this work was to investigate the antimicrobial effect of ole against major foodborne pathogens, including listeria monocytogenes, esc ... | 2017 | 28210244 |
| yope specific cd8+ t cells provide protection against systemic and mucosal yersinia pseudotuberculosis infection. | prior studies indicated that cd8+ t cells responding to a surrogate single antigen expressed by y. pseudotuberculosis, ovalbumin, were insufficient to protect against yersiniosis. herein we tested the hypothesis that cd8+ t cells reactive to the natural yersinia antigen yope would be more effective at providing mucosal protection. we first confirmed that immunization with the attenuated ksga- strain of y. pseudotuberculosis generated yope-specific cd8+ t cells. these t cells were protective agai ... | 2017 | 28207901 |
| growth of listeria monocytogenes in thawed frozen foods. | the growth characteristics of listeria monocytogenes inoculated onto frozen foods (corn, green peas, crabmeat, and shrimp) and thawed by being stored at 4, 8, 12, and 20°c were investigated. the growth parameters, lag-phase duration (lpd) and exponential growth rate (egr), were determined by using a two-phase linear growth model as a primary model and a square root model for egr and a quadratic model for lpd as secondary models, based on the growth data. the egr model predictions were compared w ... | 2017 | 28207303 |
| nanoengineered eggshell-silver tailored copolyester polymer blend film with antimicrobial properties. | in this study, the reinforcement effect of different proportions of eggshell/silver (es-ag) nanomaterial on the structural and antimicrobial properties of 70/30 poly(butylene-co-adipate terephthalate)/polylactic acid (pbat/pla) immiscible blends was investigated. the es-ag was synthesized using a single step ball milling process and characterized with x-ray diffraction (xrd), x-ray photoelectron spectroscopy (xps), and transmission electron microscopy (tem). these results confirmed the existence ... | 2017 | 28206760 |
| tim-3 inhibits macrophage control of listeria monocytogenes by inhibiting nrf2. | t cell immunoglobulin mucin-3 (tim-3) is an immune checkpoint inhibitor and its dysregulation has been related to t cell tolerance and many immune disorders, such as tumors and infection tolerance. however, the physiopathology roles of tim-3 in innate immunity remain elusive. here, we demonstrate that tim-3 inhibits macrophage phagocytosis of l. monocytogenes by inhibiting the nuclear erythroid 2-related factor 2 (nrf2) signaling pathway and increases bacterial burden. tim-3 signaling promotes n ... | 2017 | 28205579 |
| requirement of the rna-binding protein smpb during intracellular growth of listeria monocytogenes. | bacterial trans-translation is the main quality control mechanism employed to relieve stalled ribosomes. trans-translation is mediated by the small protein b (smpb) and transfer-mrna (tmrna) ribonucleoprotein complex, which interacts with translational complexes stalled at the 3' end of non-stop mrnas to release the stalled ribosomes thereby targeting the nascent polypeptides and truncated mrnas for degradation. the trans-translation system exists with a few exceptions in all bacteria. in the pr ... | 2017 | 28202229 |
| morphological change and decreasing transfer rate of biofilm-featured listeria monocytogenes egde. | listeria monocytogenes , a lethal foodborne pathogen, has the ability to resist the hostile food processing environment and thus frequently contaminates ready-to-eat foods during processing. it is commonly accepted that the tendency of l. monocytogenes ' to generate biofilms on various surfaces enhances its resistance to the harshness of the food processing environment. however, the role of biofilm formation in the transferability of l. monocytogenes egde remains controversial. we examined the g ... | 2017 | 28199146 |
| n-terminomics identifies prli42 as a membrane miniprotein conserved in firmicutes and critical for stressosome activation in listeria monocytogenes. | to adapt to changing environments, bacteria have evolved numerous pathways that activate stress response genes. in gram-positive bacteria, the stressosome, a cytoplasmic complex, relays external cues and activates the sigma b regulon. the stressosome is structurally well-characterized in bacillus, but how it senses stress remains elusive. here, we report a genome-wide n-terminomic approach in listeria that strikingly led to the discovery of 19 internal translation initiation sites and 6 miniprot ... | 2017 | 28191904 |
| assessing the capacity of growth, survival, and acid adaptive response of listeria monocytogenes during storage of various cheeses and subsequent simulated gastric digestion. | different physicochemical and microbiological characteristics of cheeses may affect listeria monocytogenes potential to grow, survive, or exhibit an acid adaptive response during storage and digestion. the objectives of the present study were to assess: i) the survival or growth potential of l.monocytogenes on various cheeses during storage, ii) the effect of initial indigenous microbiota on pathogen growth in comparison to expected growth curves retrieved by existing predictive models, and iii) ... | 2017 | 28189900 |
| invited review: hispanic-style cheeses and their association with listeria monocytogenes. | the rise in consumption of hispanic-style cheeses (hsc), due in large part to the increasing hispanic population in the united states, has not been met with advances in food safety sufficient to prevent the numerous outbreaks and recalls due to listeria monocytogenes. hispanic-style cheeses are typically high moisture and have low salt content and low acidity from being subjected to little to no ripening. these conditions necessitate refrigeration to maintain safety and quality, as the majority ... | 2017 | 28189316 |
| 32-phosphorus selectively delivered by listeria to pancreatic cancer demonstrates a strong therapeutic effect. | our laboratory has developed a novel delivery platform using an attenuated non-toxic and non-pathogenic bacterium listeria monocytogenes that infects tumor cells and selectively survives and multiplies in metastases and primary tumors with help of myeloid-derived suppressor cells (mdsc) and immune suppression in the tumor microenvironment (tme). 32p was efficiently incorporated into the listeria bacteria by starvation of the bacteria in saline, and then cultured in phosphorus-free medium complem ... | 2017 | 28186976 |
| 100k pathogen genome project: 306 listeria draft genome sequences for food safety and public health. | listeria monocytogenes is a food-associated bacterium that is responsible for food-related illnesses worldwide. this is the initial public release of 306 l. monocytogenes genome sequences as part of the 100k pathogen genome project. these isolates represent global genomic diversity in l. monocytogenes. | 2017 | 28183778 |
| comparative analysis of the bacterial membrane disruption effect of two natural plant antimicrobial peptides. | in the medicago truncatula genome about 700 genes code for nodule-specific cysteine-rich (ncr) small peptides that are expressed in the symbiotic organ, the root nodule, where they control terminal differentiation of the endosymbiotic rhizobium bacteria to nitrogen-fixing bacteroids. cationic ncr peptides were predicted to have antimicrobial activities. here antibacterial activities of ncr247, ncr335, polymyxin b (pmb), and streptomycin were investigated and compared on two foodborne pathogens s ... | 2017 | 28167938 |
| rhombencephalitis caused by listeria monocytogenes in a pastured bull. | a pastured 2-y-old cross-breed bull developed brainstem encephalitis (rhombencephalitis); listeria monocytogenes was isolated from the brain. in the brainstem, there was perivascular cuffing, multiple microabscesses, and positive immunostaining for l. monocytogenes. samples of bovine feces, water, feedstuffs, milking parlor soil, and bulk tank milk were collected from the dairy farm. seven isolates of the genus listeria were obtained, 6 of l. innocua and 1 of l. monocytogenes, which was found in ... | 2017 | 28166691 |
| assessing the genome level diversity of listeria monocytogenes from contaminated ice cream and environmental samples linked to a listeriosis outbreak in the united states. | a listeriosis outbreak in the united states implicated contaminated ice cream produced by one company, which operated 3 facilities. we performed single nucleotide polymorphism (snp)-based whole genome sequencing (wgs) analysis on listeria monocytogenes from food, environmental and clinical sources, identifying two clusters and a single branch, belonging to pcr serogroup iib and genetic lineage i. wgs cluster i, representing one outbreak strain, contained 82 food and environmental isolates from f ... | 2017 | 28166293 |
| activation and exhaustion of antigen-specific cd8(+) t cells occur in different splenic compartments during infection with plasmodium berghei. | the spleen is the major organ in which t cells are primed during infection with malaria parasites. however, little is known regarding the dynamics of the immune responses and their localization within the splenic tissue during malaria infection. we examined murine cd8(+) t cell responses during infection with plasmodium berghei using recombinant parasites expressing a model antigen ovalbumin (ova) protein and compared the responses with those elicited by listeria monocytogenes expressing the sam ... | 2017 | 28163249 |
| hot topic: antilisterial activity by endolysin plyp100 in fresh cheese. | our objective was to assess the antimicrobial efficacy of a listeria bacteriophage endolysin that may address limitations of current antilisterial processes for fresh cheeses. listeria monocytogenes is highly problematic in the manufacture and processing of ready-to-eat foods due to its environmental persistence and its ability to grow under refrigerated storage. special care must be taken to prevent listerial contamination during the production of fresh cheeses, as their delicate flavor and tex ... | 2017 | 28161174 |
| survival of listeria monocytogenes with different antibiotic resistance patterns to food-associated stresses. | the ongoing rise of antibiotic resistant microbial pathogens has become one of the major public health threats worldwide. despite all the effort and actions taken so far, a proliferation of antibiotic resistant (ar) and multi-antibiotic resistant (mar) strains is still observed, including in foodborne pathogens. this trend has been also noted recently for isolates of listeria monocytogenes, a species that, although remaining largely sensitive to clinically relevant antimicrobials, has been repor ... | 2017 | 28157581 |
| listeriolysin s: a bacteriocin from epidemic listeria monocytogenes strains that targets the microbiota. | listeria monocytogenes is a gram-positive food-borne pathogen that in humans may traverse the intestinal, placental and blood/brain barriers, causing gastroenteritis, abortions and meningitis. crossing of these barriers is dependent on the bacterial ability to enter host cells, and several l. monocytogenes surface and secreted virulence factors are known to facilitate entry and the intracellular lifecycle. the study of l. monocytogenes strains associated to human listeriosis epidemics has reveal ... | 2017 | 28156183 |
| phloretin attenuates listeria monocytogenes virulence both in vitro and in vivo by simultaneously targeting listeriolysin o and sortase a. | the critical roles of sortase a (srta) and listeriolysin o (llo) in listeria monocytogenes pathogenicity render these two virulence factors as ideal targets for the development of anti-virulence agents against l. monocytogenes infection. additionally, the structures of srta and llo are highly conserved among the members of sortase enzyme family and cholesterol dependent toxin family. here, phloretin, a natural polyphenolic compound derived from apples and pears that has little anti-l. monocytoge ... | 2017 | 28154809 |
| diversity and persistence of listeria monocytogenes within the gorgonzola pdo production chain and comparison with clinical isolates from the same area. | listeria monocytogenes causes invasive syndromes with high fatality rates in specific population groups. cheeses have been commonly implicated in outbreaks worldwide. gorgonzola is a cheese only produced in northwestern italy (it is the third italian cheese in terms of production and export) and l. monocytogenes is frequently isolated from the production chain. the aims of this study were to assess the distribution of l. monocytogenes virulence types (vts) in isolates collected in gorgonzola pro ... | 2017 | 28153561 |
| diagnosis and treatment of listeria monocytogenes endophthalmitis: a systematic review. | describe patient characteristics, treatment, and vision outcomes of listeria monocytogenes endophthalmitis, an exceedingly rare form of listeriosis. | 2017 | 28145786 |
| activity of the pore-forming virulence factor listeriolysin o is reversibly inhibited by naturally occurring s-glutathionylation. | cholesterol-dependent cytolysins (cdcs) represent a family of homologous pore-forming proteins secreted by many gram-positive bacterial pathogens. cdcs mediate membrane binding partly through a conserved c-terminal undecapeptide, which contains a single cysteine residue. while mutational changes to other residues in the undecapeptide typically have severe effects, mutation of the cysteine residue to alanine has minor effects on overall protein function. thus, the role of this highly conserved re ... | 2017 | 28138025 |
| case report: when two and ½ men go camping…. | in patients, rapid identification of bacterial species may help to guide treatment at early stages. new protocols for the identification directly from positive blood culture flasks significantly helped in the presented case report. | 2017 | 28137306 |
| axonal invasion of listeria monocytogenes: implications for early diagnosis with magnetic resonance imaging. | | 2017 | 28131231 |
| mathematical modelling of growth of listeria monocytogenes in raw chilled pork. | the aim of this study was to analyse the growth kinetics of listeria monocytogenes in naturally contaminated chilled pork. a cocktail of 26 meat-borne l. monocytogenes was inoculated to raw or sterile chilled pork to observe its growth at 4, 10, 16, 22 and 28°c respectively. the growth data were fitted by the baranyi model and ratkowsky square-root model. results showed that the baranyi model and ratkowsky square-root model could describe the growth characteristics of l. monocytogenes at differe ... | 2017 | 28129445 |
| stevia-based sweeteners as a promising alternative to table sugar: the effect on listeria monocytogenes and salmonella typhimurium growth dynamics. | sugar is commonly substituted with stevia-based products in food industry and in our daily-life. this substitution results in a change in food product characteristic formula and properties that may affect the growth dynamics of food pathogenic and spoilage bacteria. this work studies the effect of table sugar (ts), laboratory sucrose (ls), commercial stevia (st) and steviol glycosides (sg) on the growth dynamics of salmonella typhimurium and listeria monocytogenes. experiments were carried out i ... | 2017 | 28126702 |
| evaluation of ta10 broth for recovery of listeria monocytogenes from ground beef. | in 2009, the enrichment broth ta10 was released for simultaneous recovery of salmonella spp., listeria monocytogenes, and escherichia coli o157:h7. this medium was compared with other salmonella enrichment broths [lactose (lac) broth, buffered peptone water (bpw), and universal pre-enrichment (up) broth] for the recovery of heat- and freeze-injured salmonella spp. in beef by the conventional culture method. there was a significant difference between ta10 and lac enrichment broths for detecting i ... | 2017 | 28118577 |
| prevalence and genetic characteristics of meatborne listeria monocytogenes isolates from livestock farms in korea. | this study aimed to evaluate the prevalence of listeria monocytogenes on livestock farms in korea and determine their serotypes and genetic correlations. twenty-five livestock farms in korea (central: 15, south west: 7, south east: 3) were visited 2-3 times, and 2,018 samples (feces: 677, soil: 680, silage: 647, sludge: 14) were collected. samples were enriched in leb (listeria enrichment broth) and fraser broth media, and then plated on palcam agar. the isolates were identified by pcr and 16s r ... | 2016 | 28115889 |
| use of rhodomyrtus tomentosa ethanolic leaf extract for the bio-control of listeria monocytogenes post-cooking contamination in cooked chicken meat. | controlling foodborne pathogen in ready-to-eat food is important in food safety. the present study accessed the potential use of rhodomyrtus tomentosa ethanolic leaf extract as a bio-control agent against listeria monocytogenes in cooked chicken meat model system. the antilisterial activity of the plant extract was better under microwave condition and enhanced as storage temperature increased from 4 to 37 °c. the extract could reduce l. monocytogenes numbers at low (10(4) cfu/g) and high (10(6) ... | 2016 | 28115764 |
| l-glutamine induces expression of listeria monocytogenes virulence genes. | the high environmental adaptability of bacteria is contingent upon their ability to sense changes in their surroundings. bacterial pathogen entry into host poses an abrupt and dramatic environmental change, during which successful pathogens gauge multiple parameters that signal host localization. the facultative human pathogen listeria monocytogenes flourishes in soil, water and food, and in ~50 different animals, and serves as a model for intracellular infection. l. monocytogenes identifies hos ... | 2017 | 28114430 |
| an unusual presentation of listeria monocytogenes rhombencephalitis. | we describe a case of 52-year-old woman with a medical history of crohn's disease presented abrupt fever, asymmetrical multiple cranial nerve palsies and focal neurological symptoms localized to the brainstem. the patient was initially diagnosed with ischaemic stroke, because of acute clinical course and results of neuroimaging. cerebrospinal fluid analysis revealed mild infection with negative gram staining and culture. final diagnosis of listeria monocytogenes brainstem infection (rhombencepha ... | 2017 | 28110853 |
| listeria monocytogenes isolation from urine: a series of 15 cases and review. | | 2017 | 28110052 |
| naturally occurring inlb variants that support intragastric listeria monocytogenes infection in mice. | listeria monocytogenes is a causative agent of foodborne infection in humans and animals. the virulence factor inlb interacts with mammalian receptor c-met via its internalin domain to provide l. monocytogenes invasion in non-professional phagocytes. naturally occurring inlb internalin domain variants form four subclusters on the maximal likelihood tree. four variants belonging to distinct subclusters were cloned into the vector carrying 3΄ and 5΄-flanking sequences to restore full length inlb a ... | 2017 | 28104777 |
| glutathione and glutathione transferase omega 1 as key posttranslational regulators in macrophages. | macrophage activation during phagocytosis or by pattern recognition receptors, such as toll-like receptor 4, leads to the accumulation of reactive oxygen species (ros). ros act as a microbicidal defense mechanism, promoting clearance of infection, allowing for resolution of inflammation. overproduction of ros, however, overwhelms our cellular antioxidant defense system, promoting oxidation of protein machinery, leading to macrophage dysregulation and pathophysiology of chronic inflammatory condi ... | 2017 | 28102119 |
| effect of humidity and temperature on the survival of listeria monocytogenes on surfaces. | listeria monocytogenes is a pathogenic bacterium, with human disease and infection linked to dairy products, seafood, ready-to-eat meat and raw & undercooked meats. stainless steel is the most common food preparation surface and therefore, it is important to understand how food storage conditions such as surface materials, temperature and relative humidity can affect survival of l. monocytogenes. in this study, survival of l. monocytogenes on stainless steel was investigated at three temperature ... | 2017 | 28101930 |
| the influence of the toxin/antitoxin mazef on growth and survival of listeria monocytogenes under stress. | a major factor in the resilience of listeria monocytogenes is the alternative sigma factor b (σ(b)). type ii toxin/antitoxin (ta) systems are also known to have a role in the bacterial stress response upon activation via the clpp or lon proteases. directly upstream of the σ(b) operon in l. monocytogenes is the ta system mazef, which can cleave mrna at uacmu sites. in this study, we showed that the mazef ta locus does not affect the level of persister formation during treatment with antibiotics i ... | 2017 | 28098783 |
| distinct neurotoxicity profile of listeriolysin o from listeria monocytogenes. | cholesterol-dependent cytolysins (cdcs) are protein toxins that originate from gram-positive bacteria and contribute substantially to their pathogenicity. cdcs bind membrane cholesterol and build prepores and lytic pores. some effects of the toxins are observed in non-lytic concentrations. two pathogens, streptococcus pneumoniae and listeria monocytogenes, cause fatal bacterial meningitis, and both produce toxins of the cdc family-pneumolysin and listeriolysin o, respectively. it has been demons ... | 2017 | 28098781 |
| c-di-amp modulates listeria monocytogenes central metabolism to regulate growth, antibiotic resistance and osmoregulation. | cyclic diadenosine monophosphate (c-di-amp) is a conserved nucleotide second messenger critical for bacterial growth and resistance to cell wall-active antibiotics. in listeria monocytogenes, the sole diadenylate cyclase, daca, is essential in rich, but not synthetic media and δdaca mutants are highly sensitive to the β-lactam antibiotic cefuroxime. in this study, loss of function mutations in the oligopeptide importer (oppabcdf) and glycine betaine importer (gbuabc) allowed δdaca mutants to gro ... | 2017 | 28097715 |
| functional constituents of wild and cultivated goji (l. barbarum l.) leaves: phytochemical characterization, biological profile, and computational studies. | goji (lycium barbarum l.) leaves are emphasized as a functional tea or as dietary supplements. the phenolic compound profile, antioxidant, enzyme inhibitory, antimicrobial, and antimutagenic activities of leaf extracts from two selected cultivars in comparison with wild-growing plants have been evaluated. hplc-dad/esi-tof-ms analysis revealed the presence of phenolic acids and flavonoids with chlorogenic acid and rutin being the dominant compounds in the cultivated plants, whereas rutin and kaem ... | 2017 | 28095717 |
| acquired central hypoventilation following listeria monocytogenes rhombencephalitis. | | 2017 | 28087753 |
| bcap inhibits proliferation and differentiation of myeloid progenitors in the steady state and during demand situations. | b-cell adaptor for phosphatidylinositol 3-kinase (bcap) is a signaling adaptor expressed in mature hematopoietic cells, including monocytes and neutrophils. here we investigated the role of bcap in the homeostasis and development of these myeloid lineages. bcap(-/-) mice had more bone marrow (bm) monocytes than wild-type (wt) mice, and in mixed wt:bcap(-/-) bm chimeras, monocytes and neutrophils skewed toward bcap(-/-) origin, showing a competitive advantage for bcap(-/-) myeloid cells. bcap was ... | 2017 | 28087538 |
| prevalence, virulence potential, and antibiotic susceptibility profile of listeria monocytogenes isolated from bovine raw milk samples obtained from rajasthan, india. | listeriosis is a serious foodborne disease of a global concern, and can effectively be controlled by a continuous surveillance of the virulent and multidrug-resistant strains of listeria monocytogenes. this study was planned to investigate prevalence of l. monocytogenes in bovine raw milk samples. a total of 457 raw milk samples collected from 15 major cities in rajasthan, india, were analyzed for the presence of l. monocytogenes by using standard microbiological and molecular methods. five of t ... | 2017 | 28085487 |
| promyelocytic leukemia protein (pml) controls listeria monocytogenes infection. | the promyelocytic leukemia protein (pml) is the main organizer of stress-responsive subnuclear structures called pml nuclear bodies. these structures recruit multiple interactors and modulate their abundance or their posttranslational modifications, notably by the sumo ubiquitin-like modifiers. the involvement of pml in antiviral responses is well established. in contrast, the role of pml in bacterial infection remains poorly characterized. here, we show that pml restricts infection by the patho ... | 2017 | 28074026 |
| antibacterial effect of 405±5nm light emitting diode illumination against escherichia coli o157:h7, listeria monocytogenes, and salmonella on the surface of fresh-cut mango and its influence on fruit quality. | to investigate a potential of 405±5nm light emitting diode (led) as a novel technology for food preservation, the antibacterial effect of 405±5nm led on escherichia coli o157:h7, listeria monocytogenes, and salmonella spp. on the surface of fresh-cut mango and its influence on fruit quality were evaluated at different storage temperatures. led-illumination inactivated 1.0-1.6 logcfu/cm(2) of populations at 4 and 10°c for 36-48h (total dose, 2.6-3.5kj/cm(2)) regardless of bacterial species, while ... | 2017 | 28073081 |
| impact of environmental factors on the culturability and viability of listeria monocytogenes under conditions encountered in food processing plants. | the ability of listeria monocytogenes to adhere to and persist on surfaces for months or even years may be responsible for its transmission from contaminated surfaces to food products. hence the necessity to find effective means to prevent the establishment of l. monocytogenes in food processing environments. the aim of this study was to assess, through a fractional experimental design, the environmental factors that could affect the survival of l. monocytogenes cells on surfaces to thereby prev ... | 2017 | 28073080 |
| listeria monocytogenes behaviour and quality attributes during sausage storage affected by sodium nitrite, sodium lactate and thyme essential oil. | the effects of the addition of nitrite at 200 ppm (n), sodium lactate 1.5% (l) and thyme essential oil at 100 ppm (t1) on listeria monocytogenes behaviour and atpase activity inhibition were evaluated, as well as lipid oxidation through the quantification of malonaldehydes, in sausage stored at 8 ℃ for 41 days and at 30 ℃ for 14 days. the changes in the colour profile were performed during storage time at 8 ℃. quantitative descriptive sensory analyses were performed after two days at 4 ℃. at 8 ℃ ... | 2017 | 28068841 |
| listeria monocytogenes circulating in rabbit meat products and slaughterhouses in italy: prevalence data and comparison among typing results. | rabbit meat has outstanding dietetic and nutritional properties. however, few data on microbiological hazards associated with rabbit productions are available. in this study, the presence of listeria monocytogenes was determined in 430 rabbit carcasses, 256 rabbit meat cuts and products, and 599 environmental sponges collected from four italian rabbit slaughterhouses over a period of 1 year. prevalence of l. monocytogenes among the 1285 rabbit meat and environmental samples was 11%, with statist ... | 2017 | 28067541 |
| comparative genomic analysis of two serotype 1/2b listeria monocytogenes isolates from analogous environmental niches demonstrates the influence of hypervariable hotspots in defining pathogenesis. | the vast majority of clinical human listeriosis cases are caused by serotype 1/2a, 1/2b, 1/2c, and 4b isolates of listeria monocytogenes. the ability of l. monocytogenes to establish a systemic listeriosis infection within a host organism relies on a combination of genes that are involved in cell recognition, internalization, evasion of host defenses, and in vitro survival and growth. recently, whole genome sequencing and comparative genomic analysis have proven to be powerful tools for the iden ... | 2016 | 28066772 |
| comparative genomics of the listeria monocytogenes st204 subgroup. | the st204 subgroup of listeria monocytogenes is among the most frequently isolated in australia from a range of environmental niches. in this study we provide a comparative genomics analysis of food and food environment isolates from geographically diverse sources. analysis of the st204 genomes showed a highly conserved core genome with the majority of variation seen in mobile genetic elements such as plasmids, transposons and phage insertions. most strains (13/15) harbored plasmids, which altho ... | 2016 | 28066377 |
| bacterial meningitis in alcoholic patients: a population-based prospective study. | to study clinical features and outcome of community-acquired bacterial meningitis in alcoholic patients. | 2017 | 28065808 |
| hospital-acquired listeriosis linked to a persistently contaminated milkshake machine. | one case of hospital-acquired listeriosis was linked to milkshakes produced in a commercial-grade shake freezer machine. this machine was found to be contaminated with a strain of listeria monocytogenes epidemiologically and molecularly linked to a contaminated pasteurized, dairy-based ice cream product at the same hospital a year earlier, despite repeated cleaning and sanitizing. healthcare facilities should be aware of the potential for prolonged listeria contamination of food service equipmen ... | 2017 | 28065212 |
| multistate outbreak of listeria monocytogenes infections linked to whole apples used in commercially produced, prepackaged caramel apples: united states, 2014-2015. | whole apples have not been previously implicated in outbreaks of foodborne bacterial illness. we investigated a nationwide listeriosis outbreak associated with caramel apples. we defined an outbreak-associated case as an infection with one or both of two outbreak strains of listeria monocytogenes highly related by whole-genome multilocus sequence typing (wgmlst) from 1 october 2014 to 1 february 2015. single-interviewer open-ended interviews identified the source. outbreak-associated cases were ... | 2017 | 28065170 |
| evaluation of the 3m™ molecular detection assay (mda) 2 - listeria monocytogenes for the detection of listeria monocytogenes in a variety of foods and select environmental surfaces: collaborative study, first action 2016.08. | the 3m™ molecular detection assay (mda) 2 - listeria monocytogenes uses loop-mediated isothermal amplification of unique dna target sequences combined with bioluminescence to rapidly detect listeria monocytogenes in a broad range of food types and on environmental surfaces. using an unpaired study design, technicians from 13 laboratories located in the united states and canada compared the 3m mda 2 - listeria monocytogenes to the u.s. department of agriculture food safety inspection service micr ... | 2017 | 28059049 |
| evaluation of the antibacterial activity of bergamot essential oils on different listeria monocytogenes strains. | essential oils are aromatic and volatile substances extracted from plants and characterized by antimicrobial activity. the aim of the present study was to evaluate the antibacterial activity (agar disc-diffusion method) of seven different bergamot essential oils (beos) on eight listeria monocytogenes strains. minimal inhibitory concentration (mic) of most efficient beos was estimated. extremely variable results for agar disc-diffusion method for l. monocytogenes strains were reported. one of the ... | 2016 | 28058251 |
| singleton sequence type 382, an emerging clonal group of listeria monocytogenes associated with three multistate outbreaks linked to contaminated stone fruit, caramel apples, and leafy green salad. | three multistate outbreaks between 2014 and 2016, involving case patients in and outside the united states, were linked to stone fruit, caramel apples, and packaged leafy green salad contaminated with listeria monocytogenes singleton sequence type 382 (st382), a serotype ivb-v1 clone with limited genomic divergence. isolates from these outbreaks and other st382 isolates not associated with these outbreaks were analyzed by whole-genome sequencing (wgs) analysis. the primary differences among st38 ... | 2017 | 28053218 |
| structural characterisation of the fatty acid biosynthesis enzyme fabf from the pathogen listeria monocytogenes. | development of new antimicrobial agents is required against the causative agent for listeriosis, listeria monocytogenes, as the number of drug resistant strains continues to increase. a promising target is the β-ketoacyl-acyl carrier protein synthase fabf, which participates in the catalysis of fatty acid synthesis and elongation, and is required for the production of phospholipid membranes, lipoproteins, and lipopolysaccharides. in this study, we report the 1.35 å crystal structure of fabf from ... | 2017 | 28045020 |
| detection of premature stop codons leading to truncated internalin a among food and clinical strains of listeria monocytogenes. | listeria monocytogenes is a food-borne pathogen responsible for outbreaks and sporadic cases of listeriosis, a severe invasive disease. internalin a (inla) a protein encoded by inla has a key role in the mechanism of pathogenesis in l. monocytogenes infection, specifically in the invasion of human intestinal epithelial cells. studies on inla have shown that mutations leading to premature stop codons (pmscs) occur naturally and are associated with impaired virulence of l. monocytogenes strains. i ... | 2017 | 28040183 |
| application of low frequency pulsed ohmic heating for inactivation of foodborne pathogens and ms-2 phage in buffered peptone water and tomato juice. | the purpose of this study was to inactivate foodborne pathogens effectively by ohmic heating in buffered peptone water and tomato juice without causing electrode corrosion and quality degradation. escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes were used as representative foodborne pathogens and ms-2 phage was used as a norovirus surrogate. buffered peptone water and tomato juice inoculated with pathogens were treated with pulsed ohmic heating at different frequencie ... | 2017 | 28040173 |
| differential gene expression and filamentation of listeria monocytogenes 08-5923 exposed to sodium lactate and sodium diacetate. | this study reports the gene expression and filamentation in listeria monocytogenes 08-5923 following exposure to food preservatives sodium lactate (nal) and sodium diacetate (sd). l. monocytogenes 08-5923 was challenged with a mixture of nal/sd, nal or sodium acetate at 37 °c in tryptic soy broth. in the initial study, l. monocytogenes 08-5923 was exposed to nal/sd for 24 h. the transcriptome was investigated by rna sequencing. a stress response network was discovered in l. monocytogenes 08-5923 ... | 2017 | 28040163 |
| application of a 222-nm krypton-chlorine excilamp to control foodborne pathogens on sliced cheese surfaces and characterization of the bactericidal mechanisms. | this study was conducted to investigate the basic spectral properties of a 222-nm krypton-chlorine (krcl) excilamp and its inactivation efficacy against major foodborne pathogens on solid media, as well as on sliced cheese compared to a conventional 254-nm low-pressure mercury (lp hg) lamp. selective media and sliced cheese inoculated with escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes were irradiated with a krcl excilamp and a lp hg lamp at the sam ... | 2017 | 28039742 |
| endoplasmic reticulum chaperone gp96 controls actomyosin dynamics and protects against pore-forming toxins. | during infection, plasma membrane (pm) blebs protect host cells against bacterial pore-forming toxins (pfts), but were also proposed to promote pathogen dissemination. however, the details and impact of blebbing regulation during infection remained unclear. here, we identify the endoplasmic reticulum chaperone gp96 as a novel regulator of pft-induced blebbing. gp96 interacts with non-muscle myosin heavy chain iia (nmhciia) and controls its activity and remodelling, which is required for appropri ... | 2017 | 28039206 |
| an effective counterselection system for listeria monocytogenes and its use to characterize the monocin genomic region of strain 10403s. | construction of listeria monocytogenes mutants by allelic exchange has been laborious and time-consuming due to lack of proficient selection markers for the final recombination event, that is, a marker conveying substance sensitivity to the bacteria bearing it, enabling the exclusion of merodiploids and selection for plasmid loss. in order to address this issue, we engineered a counterselection marker based on a mutated phenylalanyl-trna synthetase gene (phes*). this mutation renders the phenyla ... | 2017 | 28039138 |
| a rapid typing method for listeria monocytogenes based on high-throughput multilocus sequence typing (hi-mlst). | listeria monocytogenes infects humans via food products, causing listeriosis. consequently, food companies pay meticulous attention to the risk of contamination of their products by this bacterium. while fragment analysis methods such as pulsed-field gel electrophoresis (pfge) are used to trace the sources of contamination for this bacterium, some drawbacks have been identified, namely the complexity of the methods and the difficulty of making data comparisons. as an alternative, multilocus sequ ... | 2017 | 28038334 |
| application of a puffer fish skin gelatin film containing moringa oleifera lam. leaf extract to the packaging of gouda cheese. | this study aims to develop a puffer fish skin gelatin (psg) film that contains moringa oleifera lam. leaf extract (me) as a new biodegradable film. with the increase in me concentration, the tensile strength and elongation at break of the psg film increased, whereas the oxygen permeability and water vapor permeability decreased. in addition, the psg film with me exhibited antimicrobial activity against listeria monocytogenes and antioxidant activity. to apply the me-containing psg film to food p ... | 2016 | 28035143 |
| semi-industrial microwave treatments positively affect the quality of orange-colored smoothies. | thermal processing extends the shelf life of fruit and vegetables products by inactivating microorganisms and enzymes. the effect of a pasteurization (p) treatment, 90 ± 2 °c for 35 s, provided by continuous semi-industrial microwave (mw) under different conditions (high power/short time and low power/long time) or conventional pasteurization (cp) on orange-colored smoothies and their changes throughout 45 days of storage at 5 °c were investigated. results indicated that vitamin c and antioxidan ... | 2016 | 28017984 |
| [spontaneous bacterial peritonitis with listeria monocytogenes leading to a cirrhosis discovery]. | | 2016 | 28017475 |
| sensitive detection of listeria monocytogenes based on highly efficient enrichment with vancomycin-conjugated brush-like magnetic nano-platforms. | pathogens pose a significant threat to public health worldwide. despite many technological advances in the rapid diagnosis of pathogens, sensitive pathogen detection remains challenging because target pathogenic bacteria usually exist in complex samples at very low concentrations. here, the construction of multivalent brush-like magnetic nanoprobes and their application for the efficient enriching of pathogens are demonstrated. brush-like magnetic nanoprobes were constructed by modification with ... | 2017 | 28013018 |
| thermal inactivation and sublethal injury kinetics of salmonella enterica and listeria monocytogenes in broth versus agar surface. | the objective of the present study was to compare the thermal inactivation and sublethal injury kinetics of salmonella enterica and listeria monocytogenes in broth (suspended cells) and on solid surface (agar-seeded cells). a 3-strain cocktail of s. enterica or l. monocytogenes inoculated in broth or on agar was subjected to heating in a water bath at various set temperatures (55.0, 57.5 and 60.0°c for s. enterica and 60.0, 62.5 and 65°c for l. monocytogenes). the occurrence of sublethally injur ... | 2017 | 28011300 |
| passive immunization with anti-acta and anti-listeriolysin o antibodies protects against listeria monocytogenes infection in mice. | listeria monocytogenes is an intracellular pathogen that causes listeriosis. due to its intracellular niche, l. monocytogenes has evolved to limit immune recognition and response to infection. antibodies that are slightly induced by listerial infection are completely unable to protect re-infection of l. monocytogenes. thus, a role of antibody on the protective effect against l. monocytogenes infection has been neglected for a long time. in the present study, we reported that passive immunization ... | 2016 | 28004800 |