| modeling of p-nitrophenol biodegradation by ralstonia eutropha via application of the substrate inhibition concept. | in this study, the capability of ralstonia eutropha h16 to degrade p-nitrophenol with or without a supplementary substrate (glucose or yeast extract) was investigated. using pnp as the sole energy and carbon source, the biodegradation behavior of the bacterium was modeled by applying a modified form of the monod equation that considers substrate inhibition, as suggested in the literature (mu=(mu(m)s/k(s) +s)(1-(s/s(m)(n)). pnp at a 6 mg/l initial level was degraded within 20h under the defined i ... | 2010 | 20061083 |
| identification and functional analysis of type iii effector proteins in mesorhizobium loti. | mesorhizobium loti maff303099, a microsymbiont of the model legume lotus japonicus, possesses a cluster of genes (tts) that encode a type iii secretion system (t3ss). in the presence of heterologous nodd from rhizobium leguminosarum and a flavonoid naringenin, we observed elevated expression of the tts genes and secretion of several proteins into the culture medium. inoculation experiments with wild-type and t3ss mutant strains revealed that the presence of the t3ss affected nodulation at a spec ... | 2010 | 20064065 |
| characterization of a cryptic plasmid pd403 from lactobacillus plantarum and construction of shuttle vectors based on its replicon. | a cryptic plasmid pd403 was isolated from lactobacillus plantarum d403 derived from fermented dairy products. it was 2,791 bp in size with a g+c content of 37%. nucleotide sequence analysis revealed two open reading frames, orf1 and orf2. orf1 (318 amino acids) was identified as a replication protein (repa). orf2 (137 amino acids) shared 31% similarity with the transcriptional regulator of ralstonia pickettii 12d. functional investigation indicated that orf2 (tra) had the ability of improving th ... | 2010 | 20077035 |
| expanding small-molecule functional metagenomics through parallel screening of broad-host-range cosmid environmental dna libraries in diverse proteobacteria. | the small-molecule biosynthetic diversity encoded within the genomes of uncultured bacteria is an attractive target for the discovery of natural products using functional metagenomics. phenotypes commonly associated with the production of small molecules, such as antibiosis, altered pigmentation, or altered colony morphology, are easily identified from screens of arrayed metagenomic library clones. however, functional metagenomic screening methods are limited by their intrinsic dependence on a h ... | 2010 | 20081001 |
| experimental evolution of a plant pathogen into a legume symbiont. | rhizobia are phylogenetically disparate alpha- and beta-proteobacteria that have achieved the environmentally essential function of fixing atmospheric nitrogen in symbiosis with legumes. ample evidence indicates that horizontal transfer of symbiotic plasmids/islands has played a crucial role in rhizobia evolution. however, adaptive mechanisms that allow the recipient genomes to express symbiotic traits are unknown. here, we report on the experimental evolution of a pathogenic ralstonia solanacea ... | 2010 | 20084095 |
| aluminum uptake and disease resistance in nicotiana rustica leaves. | the comparative effectiveness of aluminum hydroxide and aluminum chloride has been studied in the development of bacterial wilt infection on leaves of nicotiana rustica cv. gansu yellow flower. we have analyzed the changes of foliar h(2)o(2) content, as well as of non-enzymatic and enzymatic antioxidants under aluminum stress. pretreatment with aluminum hydroxide before pathogen challenge reduced the development of ralstonia solanacearum infection and decreased the extent of leaf injury. the pre ... | 2010 | 20106526 |
| complete genome sequence of the multiresistant taxonomic outlier pseudomonas aeruginosa pa7. | pseudomonas aeruginosa pa7 is a non-respiratory human isolate from argentina that is multiresistant to antibiotics. we first sequenced gyra, gyrb, parc, pare, ampc, ampr, and several housekeeping genes and found that pa7 is a taxonomic outlier. we report here the complete sequence of the 6,588,339 bp genome, which has only about 95% overall identity to other strains. pa7 has multiple novel genomic islands and a total of 51 occupied regions of genomic plasticity. these islands include antibiotic ... | 2010 | 20107499 |
| identification of a bifunctional udp-4-keto-pentose/udp-xylose synthase in the plant pathogenic bacterium ralstonia solanacearum strain gmi1000, a distinct member of the 4,6-dehydratase and decarboxylase family. | the udp-sugar interconverting enzymes involved in udp-glca metabolism are well described in eukaryotes but less is known in prokaryotes. here we identify and characterize a gene (rsu4kpxs) from ralstonia solanacearum str. gmi1000, which encodes a dual function enzyme not previously described. one activity is to decarboxylate udp-glucuronic acid to udp-beta-l-threo-pentopyranosyl-4''-ulose in the presence of nad(+). the second activity converts udp-beta-l-threo-pentopyranosyl-4''-ulose and nadh t ... | 2010 | 20118241 |
| s-glycoprotein-like protein regulates defense responses in nicotiana plants against ralstonia solanacearum. | rsrga4 (for ralstonia solanacearum-responsive gene a4) encodes a polypeptide similar to s-locus glycoprotein (sgp) from brassica rapa and sgp-like proteins from ipomoea trifida and medicago truncatula. therefore, we designated rsrga4 as ntsglp (for nicotiana tabacum sgp-like protein) and nbsglp (its nicotiana benthamiana ortholog). nbsglp is expressed in root, leaf, petal, gynoecium, and stamen. expression of nbsglp was strongly induced by inoculation with an avirulent strain of r. solanacearum ... | 2010 | 20118275 |
| genome-wide identification of a large repertoire of ralstonia solanacearum type iii effector proteins by a new functional screen. | the gram-negative plant-pathogenic bacterium ralstonia solanacearum utilizes the hypersensitive response and pathogenicity (hrp) type iii secretion system (t3ss) to cause disease in plants. to determine the entire repertoire of effector proteins possessed by r. solanacearum rs1000, we constructed a transposon carrying a calmodulin-dependent adenylate cyclase reporter that can be used to specifically detect rip (ralstonia protein injected into plant cells) genes by monitoring the camp level in pl ... | 2010 | 20121447 |
| pamdb, a multilocus sequence typing and analysis database and website for plant-associated microbes. | although there are adequate dna sequence differences among plant-associated and plant-pathogenic bacteria to facilitate molecular approaches for their identification, identification at a taxonomic level that is predictive of their phenotype is a challenge. the problem is the absence of a taxonomy that describes genetic variation at a biologically relevant resolution and of a database containing reference strains for comparison. moreover, molecular evolution, population genetics, ecology, and epi ... | 2010 | 20128693 |
| monitoring growth and movement of ralstonia solanacearum cells harboring plasmid prss12 derived from bacteriophage phirss1. | we monitored growth and movement of ralstonia solanacearum harboring the plasmid prss12 in tomato seedlings. the plasmid contains a gene for green fluorescent protein (gfp) and is stably maintained in r. solanacearum cells without selection pressure. bacteria harboring the plasmid can be tracked in planta by visualizing gfp fluorescence. stems of seedlings were infected with r. solanacearum cells transformed with prss12, and bacterial growth and movement, particularly around the vascular bundles ... | 2010 | 20129100 |
| microbial population dynamics during startup of a full-scale anaerobic digester treating industrial food waste in kyoto eco-energy project. | the microbial community in a full-scale anaerobic digester (2300m3) treating industrial food waste in the kyoto eco-energy project was analyzed using terminal restriction fragment length polymorphism for eubacterial and archaeal 16s rrna genes. both thermophilic and mesophilic sludge of treated swine waste were seeded to the digestion tank. during the 150-day startup period, coffee grounds as a main food waste, along with potato, kelp and boiled beans, tofu, bean curd lees, and deep-fried bean c ... | 2010 | 20129780 |
| a real-time pcr assay for the quantitative detection of ralstonia solanacearum in the horticultural soil and plant tissues. | a specific and rapid real-time pcr assay for detecting ralstonia solanacearum in the horticultural soil and plant tissues was developed in this study. the specific primers rsf/rsr were designed based on the upstream region of udp-3-o-acyl-glcnac deacetylase gene from r. solanacearum, and a pcr product of 159 bp was amplified specifically from 28 strains of r. solanacearum, which represent all genetically diverse alui types and all 6 biovars, but not from any other nontarget species. the detectio ... | 2010 | 20134252 |
| statistical medium optimization and biodegradative capacity of ralstonia eutropha toward p-nitrophenol. | the effect of p-nitrophenol (pnp) concentration with or without glucose and yeast extract on the growth and biodegradative capacity of ralstonia eutropha was examined. the chemical constituents of the culture medium were modeled using a response surface methodology. the experiments were performed according to the central composite design arrangement considering pnp, glucose and yeast extract as the selected variables whose influences on the degradation was evaluated (shaking in reciprocal mode, ... | 2010 | 20140697 |
| efficient biocatalytic production of d-4-hydroxyphenylglycine by whole cells of recombinant ralstonia pickettii. | the first establishment of a homologous expression system in the host ralstonia pickettii cgmcc1596 using the compatible broad-host-range plasmid pwb5 is described. when whole cells of the recombinant strain r. pickettii mmyy01 (cgmcc1596/pyy05) were used as the biocatalyst to transform dl-4-hydroxyphenylhydantoin (dl-hph) to d-4-hydroxyphenylglycine (d-hpg), the conversion rate reached 94 % in first 9 h, at a production rate of 2.8 g l(-1) h(-1), with the rapid reduction of the intermediate [n- ... | 2010 | 20140718 |
| 2,3-dihydroxypropane-1-sulfonate degraded by cupriavidus pinatubonensis jmp134: purification of dihydroxypropanesulfonate 3-dehydrogenase. | 2,3-dihydroxypropane-1-sulfonate (dhps) is a widespread intermediate in plant and algal transformations of sulfoquinovose (sq) from the plant sulfolipid sulfoquinovosyl diacylglycerol. further, dhps is recovered quantitatively during bacterial degradation of sq by klebsiella sp. strain abr11. dhps is also a putative precursor of sulfolactate in e.g. ruegeria pomeroyi dss-3. a bioinformatic approach indicated that some 28 organisms with sequenced genomes might degrade dhps inducibly via sulfolact ... | 2010 | 20150239 |
| requirements for construction of a functional hybrid complex of photosystem i and [nife]-hydrogenase. | the development of cellular systems in which the enzyme hydrogenase is efficiently coupled to the oxygenic photosynthesis apparatus represents an attractive avenue to produce h(2) sustainably from light and water. here we describe the molecular design of the individual components required for the direct coupling of the o(2)-tolerant membrane-bound hydrogenase (mbh) from ralstonia eutropha h16 to the acceptor site of photosystem i (ps i) from synechocystis sp. pcc 6803. by genetic engineering, th ... | 2010 | 20154103 |
| comparison of the membrane-bound [nife] hydrogenases from r. eutropha h16 and d. vulgaris miyazaki f in the oxidized ready state by pulsed epr. | the geometric and electronic structures of the active sites in the oxidized ni(r)-b state of the [nife] hydrogenases from ralstonia eutropha h16 and desulfovibrio vulgaris miyazaki f were investigated in pulsed epr and endor experiments at two different microwave frequencies (x- and q-band). two hyperfine-couplings were clearly resolved in the frozen solution spectra arising from the beta-protons of the nickel-coordinating cysteine residues cys549 and cys586 from the desulfovibrio vulgaris and r ... | 2010 | 20165762 |
| adjustable mutations in lactate (la)-polymerizing enzyme for the microbial production of la-based polyesters with tailor-made monomer composition. | lactate (la)-polymerizing enzyme (lpe) is a newly established class of polyhydroxyalkanoate (pha) synthase, which can incorporate la units into a polymer chain. we previously synthesized p(la-co-3-hydroxybutyrate)s [p(la-co-3hb)s] in recombinant escherichia coli using the first lpe, which is the ser325thr/glu481lys mutant of pha synthase from pseudomonas sp. 61-3 [phac1(ps)st/qk]. in this study, we finely regulated la fraction in the copolymer by saturated mutations at position 392 (f392x), whic ... | 2010 | 20166718 |
| h2 conversion in the presence of o2 as performed by the membrane-bound [nife]-hydrogenase of ralstonia eutropha. | [nife]-hydrogenases catalyze the oxidation of h(2) to protons and electrons. this reversible reaction is based on a complex interplay of metal cofactors including the ni-fe active site and several [fe-s] clusters. h(2) catalysis of most [nife]-hydrogenases is sensitive to dioxygen. however, some bacteria contain hydrogenases that activate h(2) even in the presence of o(2). there is now compelling evidence that o(2) affects hydrogenase on three levels: 1) h(2) catalysis, 2) hydrogenase maturation ... | 2010 | 20186906 |
| effects of bovine milk lactoperoxidase system on some bacteria. | bovine lactoperoxidase (lpo) was purified from skimmed milk using amberlite cg-50-h+ resin, cm sephadex c-50 ion-exchange chromatography, and sephadex g-100 gel filtration chromatography. lactoperoxidase was purified 20.45-fold with a yield of 28.8%. purity of enzyme checked by sodium dodecyl sulphate-polyacrylamide gel electrophoresis method and a single band was observed. km was 0.25 mm at 20 degrees c, vmax value was 7.95 micromol/ml min at 20 degrees c (ph 6.0). antibacterial study was done ... | 2010 | 20198919 |
| mosaic structure of intragenic repetitive elements in histone h1-like protein hc2 varies within serovars of chlamydia trachomatis. | the histone-like protein hc2 binds dna in chlamydia trachomatis and is known to vary in size between 165 and 237 amino acids, which is caused by different numbers of lysine-rich pentamers. a more complex structure was seen in this study when sequences from 378 specimens covering the hctb gene, which encodes hc2, were compared. | 2010 | 20236532 |
| delsgate: a robust and rapid method for gene deletion. | gene deletion is one of the most powerful tools to study gene function. in the genomics era there is great demand for fast, simple high-throughput methods for gene deletion to study the roles of the large numbers of genes that are being identified. here we present an approach that speeds up the process of generation of deletion mutants by greatly simplifying the production of gene deletion constructs. with this purpose we have developed a method, which we named delsgate (deletion via gateway), t ... | 2010 | 20238261 |
| production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from unrelated carbon sources by metabolically engineered escherichia coli. | a metabolically engineered escherichia coli has been constructed for the production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [p(3hb-co-4hb)] from unrelated carbon sources. genes involved in succinate degradation in clostridium kluyveri and p(3hb) accumulation pathway of ralstonia eutropha were co-expressed for the synthesis of the above copolyester. e. coli native succinate semialdehyde dehydrogenase genes sad and gabd were both deleted for eliminating succinate formation from succinate s ... | 2010 | 20304089 |
| strains of internal biofilm in aerobic granular membrane bioreactors. | this study isolated strains in suspended liquor, the surface fouling layer, and biofilm inside hollow-fiber membranes of a membrane bioreactor (mbr); analyzed their distributions, sizes, surface charges, and growth behaviors; and determined the quantities of extracellular polymeric substances (eps) secreted by these strains under different organic loadings. three strains, which may penetrate the microfiltration membranes, were close relatives of the ralstonia mannitolilytica strain sdv (genbank ... | 2010 | 20306189 |
| the complete multipartite genome sequence of cupriavidus necator jmp134, a versatile pollutant degrader. | cupriavidus necator jmp134 is a gram-negative beta-proteobacterium able to grow on a variety of aromatic and chloroaromatic compounds as its sole carbon and energy source. | 2010 | 20339589 |
| protein-protein complex formation affects the ni-fe and fe-s centers in the h2-sensing regulatory hydrogenase from ralstonia eutropha h16. | the regulatory ni-fe hydrogenase (rh) from the h(2)-oxidizing bacterium ralstonia eutropha functions as an oxygen-resistant hydrogen sensor, which is composed of the large, active-site-containing hoxc subunit and the small subunit hoxb carrying fe-s clusters. in vivo, the hoxbc subunits form a dimer designated as rh(wt). the rh(wt) protein transmits its signals to the histidine protein kinase hoxj, which itself forms a homotetramer and a stable complex with rh(wt) (rh(wt)-hoxj(wt)), located in t ... | 2010 | 20340124 |
| biofilm bacterial communities and abundance in a full-scale drinking water distribution system in shanghai. | community diversity and abundance of biofilms from a full-scale drinking water distribution system in shanghai were characterized by denaturing gradient gel electrophoresis (dgge) analysis of 16s rrna sequences and heterotrophic plate count (hpc), respectively. bacteria affiliated to the beta- and gamma-proteobacteria were dominating in both in-situ and hpc-culturable bacterial communities. other bacteria present included members of alphaproteobacteria, bacteroides, actinobacteria, nitrospirae a ... | 2010 | 20375488 |
| impact of amino acid substitutions near the catalytic site on the spectral properties of an o2-tolerant membrane-bound [nife] hydrogenase. | [nife] hydrogenases are widespread among microorganisms and catalyze the reversible cleavage of molecular hydrogen. however, only a few bacteria, such as ralstonia eutropha h16 (re), synthesize [nife] hydrogenases that perform h(2) cycling in the presence of o(2). these enzymes are of special interest for biotechnological applications. to gain further insight into the mechanism(s) responsible for the remarkable o(2) tolerance, we employ ftir and epr spectroscopy to study mutant variants of the m ... | 2010 | 20376875 |
| crystallization and preliminary x-ray crystallographic analysis of the [nife]-hydrogenase maturation factor hypf1 from ralstonia eutropha h16. | the hydrogenase maturation factor hypf1 is a truncated but functional version of the hypf protein. hypf is known to be involved in the supply of the cn(-) ligands of the active site of [nife]-hydrogenases, utilizing carbamoyl phosphate as a substrate. the first crystallization and preliminary x-ray studies of hypf1 from ralstonia eutropha h16 are reported here. crystals of hypf1 (394 amino acids, 40.7 kda) were obtained by the sitting-drop vapour-diffusion technique using sodium formate as a pre ... | 2010 | 20383020 |
| novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of xanthomonas fuscans subsp. aurantifolii. | citrus canker is a disease that has severe economic impact on the citrus industry worldwide. there are three types of canker, called a, b, and c. the three types have different phenotypes and affect different citrus species. the causative agent for type a is xanthomonas citri subsp. citri, whose genome sequence was made available in 2002. xanthomonas fuscans subsp. aurantifolii strain b causes canker b and xanthomonas fuscans subsp. aurantifolii strain c causes canker c. | 2010 | 20388224 |
| [cloning and molecular organization of the polyhydroxyalkanoic acid synthase gene (phac) of ralstonia eutropha strain b5786]. | class i polyhydroxyalkanoic acid (pha) synthase gene (phac) of ralstonia eutropha strain b5786 was cloned and characterized. r. eutropha b5786 features the ability to synthesize multicomponent phas with short- and medium-chain-length monomers from simple carbohydrate substrate. a correlation was made between the molecular structure of pha synthase and substrate specificity and the ability of strain-producers to accumulate phas of this or that structure. a strong similarity of pha synthase of r. ... | 2010 | 20391757 |
| genome-wide transcriptome analyses of the 'knallgas' bacterium ralstonia eutropha h16 with regard to polyhydroxyalkanoate metabolism. | ralstonia eutropha h16 is probably the best-studied 'knallgas' bacterium and producer of poly(3-hydroxybutyrate) (phb). genome-wide transcriptome analyses were employed to detect genes that are differentially transcribed during phb biosynthesis. for this purpose, four transcriptomes from different growth phases of the wild-type h16 and of the two phb-negative mutants phb(-)4 and delta phac1 were compared: (i) cells from the exponential growth phase with cells that were in transition to stationar ... | 2010 | 20395272 |
| [genetic variability of the bacterium ralstonia solanacearum (burkholderiales: burholderiaceae) in the banana-growing region of uraba (colombia)]. | the banana moko disease, caused by the bacterium ralstonia solanacearum, is one of the most important phytopathological problems of the banana agribusiness in tropical countries. in uraba and magdalena (colombia), the main exporting regions of banana in colombia, this disease causes a destruction estimated in 16.5 ha/year. the bacterium presents an extremely high level of genetic variation that affects control measures. this is the first study of its variation in colombia and was done with aflp ... | 2010 | 20411705 |
| biochemical characterization of gram-positive and gram-negative plant-associated bacteria with micro-raman spectroscopy. | raman spectra of gram-positive and gram-negative plant bacteria have been measured with micro-raman spectrometers equipped with 785 and 514.5 nm lasers. the gram-positive bacteria microbacterium testaceum, paenibacillus validus, and clavibacter michiganensis subsp. michiganensis have strong carotenoid bands in the regions 1155-1157 cm(-1) and 1516-1522 cm(-1) that differentiate them from other tested gram-negative bacteria. in the raman spectrum of gram-positive bacteria bacillus megaterium exci ... | 2010 | 20412629 |
| biofilms formed on humic substances: response to flow conditions and carbon concentrations. | stream biofilms are exposed to dynamic conditions of flow velocity and organic carbon availability. thus, the aim of this study was to investigate the response of biofilms formed with and without humic substances (hss) to an increase in flow velocity (0.04-0.10 ms(-1)) and hss concentration (9.7+/-1.0 to 19.8+/-0.4 mgl(-1) c). the highest amount of biofilm, measured as volatile suspended solids and total countable cells, was observed at 0.10 m s(-1) without hss. the bacterial community compositi ... | 2010 | 20413305 |
| the small subunit arob of arsenite oxidase: lessons on the [2fe-2s] rieske protein superfamily. | here, we describe the characterization of the [2fe-2s] clusters of arsenite oxidases from rhizobium sp. nt-26 and ralstonia sp. 22. both reduced rieske proteins feature epr signals similar to their homologs from rieske-cyt b complexes, with g values at 2.027, 1.88, and 1.77. redox titrations in a range of ph values showed that both [2fe-2s] centers have constant e(m) values up to ph 8 at approximately +210 mv. above this ph value, the e(m) values of both centers are ph-dependent, similar to what ... | 2010 | 20421651 |
| arsenite oxidase from ralstonia sp. 22: characterization of the enzyme and its interaction with soluble cytochromes. | we characterized the aro arsenite oxidation system in the novel strain ralstonia sp. 22, a beta-proteobacterium isolated from soil samples of the salsigne mine in southern france. the inducible aro system consists of a heterodimeric membrane-associated enzyme reacting with a dedicated soluble cytochrome c(554). our biochemical results suggest that the weak association of the enzyme to the membrane probably arises from a still unknown interaction partner. analysis of the phylogeny of the aro gene ... | 2010 | 20421652 |
| comonomer compositional distribution, physical properties, and enzymatic degradability of bacterial poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) copolyesters. | poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) (p(3hb-co-3h4mv)) was synthesized by using ralstonia eutropha strain phb(-)4 (pha-negative mutant) harboring with the n149s/d171g double mutation of pha synthase gene from aeromonas caviae (phac(ac) nsdg mutant) with 4-methylvaleric acid and fructose as the carbon sources. because the microbially synthesized p(3hb-co-3h4mv) samples were found to have broad comonomer compositional distribution, p(3hb-co-3h4mv) samples with 3h4mv content of 7, ... | 2010 | 20441146 |
| popw of ralstonia solanacearum, a new two-domain harpin targeting the plant cell wall. | harpins are extracellular glycine-rich proteins eliciting a hypersensitive response (hr). in this study, we identified a new harpin, popw, from ralstonia solanacearum strain zj3721. this 380-amino-acid protein is acidic, rich in glycine and serine, and lacks cysteine. when infiltrated into the leaves of tobacco (non-host), popw induced a rapid tissue collapse via a heat-stable but protease-sensitive hr-eliciting activity. popw has an n-terminal harpin domain (residues 1-159) and a c-terminal pec ... | 2010 | 20447285 |
| a new shuttle vector for gene expression in biopolymer-producing ralstonia eutropha. | ralstonia eutropha (formerly alcaligenes eutrophus) is a fascinating microorganism with a great scientific importance and an immense commercial potential. a new genetic transformation system for the organism would greatly facilitate the biological study and molecular engineering of this organism. we report here a versatile gene expression method for the genetic engineering of r. eutropha. this method, based on a simplified electroporation protocol, uses a recombinant plasmid, pbs29-p2, containin ... | 2010 | 20447426 |
| isolation of phylogenetically diverse nonylphenol ethoxylate-degrading bacteria and characterization of their corresponding biotransformation pathways. | most nonylphenol ethoxylate (npeo)-degrading isolates have been assigned to gamma-proteobacteria, which is different from the results acquired by using molecular ecological techniques. to better understand the environmental fate of npeos, bacterial isolation strategy characterized by the use of gellan gum as a gelling reagent and a low concentration of target carbon source were used to isolate phylogenetically diverse npeo-degrading bacteria from activated sludge, and the biotransformation pathw ... | 2010 | 20457466 |
| isolation and characterization of 3,5,6-trichloro-2-pyridinol-degrading ralstonia sp. strain t6. | a 3,5,6-trichloro-2-pyridinol (tcp)-degrading strain, t6, was isolated by continuous enrichment culture and identified as ralstonia sp. based on morphological, physiological and biochemical tests as well as 16s rdna sequence analysis. the bacterium metabolized 100 mg/l tcp within 12 h and 700 mg/l tcp in 80 h. a green metabolite, putatively identified as 3,6-dihydroxypyridine-2,5-dione, was detected. this is the first report of tcp-degrading isolate from the genus of ralstonia. strain t6 could p ... | 2010 | 20457514 |
| isolation and characterization of fenamiphos degrading bacteria. | the biological factors responsible for the microbial breakdown of the organophosphorus nematicide fenamiphos were investigated. microorganisms responsible for the enhanced degradation of fenamiphos were isolated from soil that had a long application history of this nematicide. bacteria proved to be the most important group of microbes responsible for the fenamiphos biodegradation process. seventeen bacterial isolates utilized the pure active ingredient fenamiphos as a carbon source. sixteen isol ... | 2010 | 20464454 |
| a putative genomic island, pgi-1, in ralstonia solanacearum biovar 2 revealed by subtractive hybridization. | ralstonia solanacearum biovar 2, a key bacterial pathogen of potato, has recently established in temperate climate waters. on the basis of isolates obtained from diseased (potato) plants, its genome has been assumed to be virtually clonal, but information on environmental isolates has been lacking. based on differences in pulsed-field gel electrophoresis patterns, we compared the genomes of two biovar 2 strains with different life histories. thus, genomic dna of the novel environmental strain kz ... | 2010 | 20467813 |
| analysis of cell wall proteins regulated in stem of susceptible and resistant tomato species after inoculation with ralstonia solanacearum: a proteomic approach. | proteomics approach was used to elucidate the molecular interactions taking place at the stem cell wall level when tomato species were inoculated with ralstonia solanacearum, a causative agent of bacterial wilt. cell wall proteins from both resistant and susceptible plants before and after the bacterial inoculation were extracted from purified cell wall with salt buffers and separated with 2-d ief/sds-page and with 3-d ief/sds/sds-page for basic proteins. the gels stained with colloidal coomassi ... | 2010 | 20496099 |
| incidence, relevance and response for ralsfonia respiratory infections. | cases of ralstonia colonization/infection occasionally reported by hospitals has generated increased interest in an organism previously little known to most clinicians. our goal was to determine the incidence of respiratory colonizations and infections involving ralstonia and the association of mechanical ventilation (limited to reports on respiratory-related occurrences in the u.s.a.) and propose a decision chart to assist response. | 2010 | 20499534 |
| [identification, colonization and disease prevention capacity of an antagonistic bacterium against ralstonia solanacearum]. | to isolate a bacterial strain ypp-9, dominantly colonizing the rhizosphere of tomato using root exudate medium. in this study, we investigated the antagnism and disease-controling effect against ralstonia solanacearum, evaluated the ability to colonize the rhizosphere of tomato, and further analyzed the phylogeny of ypp-9. | 2010 | 20499639 |
| optimization of growth media components for polyhydroxyalkanoate (pha) production from organic acids by ralstonia eutropha. | we employed systematic mixture analysis to determine optimal levels of acetate, propionate, and butyrate for cell growth and polyhydroxyalkanoate (pha) production by ralstonia eutropha h16. butyrate was the preferred acid for robust cell growth and high pha production. the 3-hydroxyvalerate content in the resulting pha depended on the proportion of propionate initially present in the growth medium. the proportion of acetate dramatically affected the final ph of the growth medium. a model was con ... | 2010 | 20535466 |
| efficacy of various chemical disinfectants on biofilms formed in spacecraft potable water system components. | as the provision of potable water is critical for successful habitation of the international space station (iss), life support systems were installed in december 2008 to recycle both humidity from the atmosphere and urine to conserve available water in the station. in-flight pre-consumption testing from the dispensing needle at the potable water dispenser (pwd) indicated that bacterial concentrations exceeded the current iss specifications of 50 colony-forming units (cfu) ml(-1). subsequent inve ... | 2010 | 20544435 |
| genomes of three tomato pathogens within the ralstonia solanacearum species complex reveal significant evolutionary divergence. | the ralstonia solanacearum species complex includes thousands of strains pathogenic to an unusually wide range of plant species. these globally dispersed and heterogeneous strains cause bacterial wilt diseases, which have major socio-economic impacts. pathogenicity is an ancestral trait in r. solanacearum and strains with high genetic variation can be subdivided into four phylotypes, correlating to isolates from asia (phylotype i), the americas (phylotype iia and iib), africa (phylotype iii) and ... | 2010 | 20550686 |
| functional expression of phacab genes from cupriavidus taiwanensis strain 184 in escherichia coli for polyhydroxybutyrate production. | polyhydroxyalkanoates are polyesters synthesized by numerous microorganisms. these polyesters are biodegradable and have similar properties to those of conventional plastics. cupriavidus taiwanensis strain 184 is phylogenetically related to the well-known polyhydroxybutyrate (phb) producer ralstonia eutropha (cupriavidus necator) and is also shown to be able to accumulate significant amounts of phb. in this study, we cloned the phb synthesis genes (phacab) from c. taiwanensis 184 into escherichi ... | 2010 | 20556541 |
| ralstonia solanacearum: secrets of a major pathogen unveiled by analysis of its genome. | summary ralstonia solanacearum taxonomy: bacteria; proteobacteria; beta subdivision; ralstonia group; genus ralstonia microbiological properties: gram-negative, aerobic, motile rod. disease symptoms: agent of bacterial wilt of solanaceous plants, which appears as a sudden wilt. typically, stem cross-sections ooze a whitish bacterial exudate. r. solanacearum is also the agent of the moko disease of banana and brown rot of potato. disease control: pathogen-free seed and transplants. few resistant ... | 2002 | 20569316 |
| ralstonia solanacearum ampd is required for wild-type bacterial wilt virulence. | abstract a gene resembling enterobacterial ampd was identified in the bacterial wilt pathogen, ralstonia solanacearum. the gene lies 13 bp 3' of pehsr, a two-component positive regulator of virulence factors such as plant cell wall-degrading polygalacturonases and bacterial motility. ampd, an n-acetylmuramyl-l-alanine amidase, degrades and recycles bacterial cell wall components and also plays a role in the induction of beta-lactamase, which confers ampicillin resistance. ampd is probably not in ... | 2000 | 20572964 |
| inhibitory effects of hinokitiol on tyrosinase activity and melanin biosynthesis and its antimicrobial activities. | the inhibitory effects of hinokitiol, a constituent of the woody oils isolated from cupressaceae heartwood, on mushroom tyrosinase and melanin formation in b16 melanoma cells as well as its antimicrobial activity were investigated. our results showed that hinokitiol could strongly inhibit both monophenolase activity and diphenolase activity of the enzyme and the inhibition was reversible. the ic(50) values were estimated as 9.67 μm for monophenolase activity and 0.21 μm for diphenolase activity. ... | 2010 | 20578978 |
| microbial production of acetoacetate by recombinant escherichia coli. | a synthetic pathway consisting of octab encoding succinyl-coa:acetoacetate coa-transferase and phaa encoding beta-ketothiolase from ralstonia eutropha h16 was constructed in escherichia coli for extracellular production of acetoacetate. the recombinant e. coli produced 8.2g/l acetoacetate in shake flasks at 37 degrees c in 24h. in a 6-l fermentor, extracellular acetoacetate production was enhanced to 13.1g/l after 30h of fed-batch growth. to our knowledge, this is the first study on microbial pr ... | 2010 | 20591654 |
| impact of multiple beta-ketothiolase deletion mutations in ralstonia eutropha h16 on the composition of 3-mercaptopropionic acid-containing copolymers. | beta-ketothiolases catalyze the first step of poly(3-hydroxybutyrate) [poly(3hb)] synthesis in bacteria by condensing two molecules of acetyl coenzyme a (acetyl-coa) to acetoacetyl-coa. analyses of the genome sequence of ralstonia eutropha h16 revealed 15 isoenzymes of phaa in this bacterium. in this study, we generated knockout mutants of various phaa homologues to investigate their role in and contributions to poly(3hb) metabolism and to suppress biosynthesis of 3hb-coa for obtaining enhanced ... | 2010 | 20601511 |
| development of a gene knockout system for ralstonia eutropha h16 based on the broad-host-range vector expressing a mobile group ii intron. | ralstonia eutropha h16 is a gram-negative lithoautotrophic bacterium and is one of the best biopolymer-producing bacteria. it can grow to high cell densities either under lithoautotrophic or under heterotrophic conditions, which makes it suitable for a number of biotechnological applications. also, r. eutropha h16 can degrade various aromatic compounds for environmental applications. the mobile group ii intron can be used for the rapid and specific disruption of various bacterial genes by insert ... | 2010 | 20608980 |
| a cbb(3)-type cytochrome c oxidase contributes to ralstonia solanacearum r3bv2 growth in microaerobic environments and to bacterial wilt disease development in tomato. | ralstonia solanacearum race 3 biovar 2 (r3bv2) is an economically important soilborne plant pathogen that causes bacterial wilt disease by infecting host plant roots and colonizing the xylem vessels. little is known about r3bv2 behavior in the host rhizosphere and early in bacterial wilt pathogenesis. to explore this part of the disease cycle, we used a novel taxis-based promoter-trapping strategy to identify pathogen genes induced in the plant rhizosphere. this screen identified several rex (ro ... | 2010 | 20615115 |
| mapk phosphatase mkp2 mediates disease responses in arabidopsis and functionally interacts with mpk3 and mpk6. | mitogen-activated protein kinase (mapk) cascades have important functions in plant stress responses and development and are key players in reactive oxygen species (ros) signalling and in innate immunity. in arabidopsis, the transmission of ros and pathogen signalling by mapks involves the coordinated activation of mpk6 and mpk3; however, the specificity of their negative regulation by phosphatases is not fully known. here, we present genetic analyses showing that mapk phosphatase 2 (mkp2) regula ... | 2010 | 20626661 |
| ralstonia solanacearum induces soluble amine-oxidase activity in solanum torvum stem calli. | solanum torvum is reported to carry resistance to bacterial wilt caused by ralstonia solanacearum. so, this wild species is used as rootskock for eggplants or tomatoes in naturally infected soil. this study aimed to investigate the involvement of the polyamine metabolism pathway in the resistance mechanisms of this species. calli induced from solanum torvum stem explants were inoculated with the bacteria under partial vacuum. all calli showed a hypersensitive response after infiltration. further ... | 2010 | 20650643 |
| biosequestration via cooperative binding of copper by ralstonia pickettii. | ralstonia pickettii isolated from copper-contaminated lake sediment are adapted to high levels of copper after 100 years of selective pressure. two r. pickettii strains (12d and 12j) were selected for the studies reported herein due to their distinct differences in genomic structure, different metal resistance patterns and carriage of a filamentous phage. copper sequestration studies revealed that these strains could bind up to 27.44 (12d) and 38.19 (12j) mg copper per g dry weight of cells and ... | 2010 | 20662391 |
| deciphering phenotypic diversity of ralstonia solanacearum strains pathogenic to potato. | based on the phylotype classification, we questioned how genetically and phenotypically diverse strains of ralstonia solanacearum pathogenic to potato may be. we studied 129 european and mediterranean strains along with 57 reference strains known to cover genetic diversity in this species. phylogeny analysis was done on endoglucanase gene sequences. pathogenicity to potato, tomato, and eggplant was established at 24 to 30°c and 15 to 24°c, whereas tests on banana were conducted at 24 to 30°c. th ... | 2010 | 20672871 |
| molecular traits controlling host range and adaptation to plants in ralstonia solanacearum. | ralstonia solanacearum is regarded as one of the world's most important bacterial plant pathogens because of its aggressiveness, large host range, broad geographical distribution and long persistence in soil and water environments. this root pathogen is an attractive model to investigate the question of host adaptation as it exhibits a remarkably broad host range, being able to infect numerous plant species belonging to different botanical families. several effector proteins transiting through t ... | 2010 | 20673287 |
| [biodiversity of phosphate-dissolving and plant growth--promoting endophytic bacteria of two crops]. | we isolated and characterized phosphate-dissolving endophytic bacteria from two commonly cultivated crops. | 2010 | 20687333 |
| a competitive index assay identifies several ralstonia solanacearum type iii effector mutant strains with reduced fitness in host plants. | ralstonia solanacearum, the causal agent of bacterial wilt, is a soil bacterium which can naturally infect a wide range of host plants through the root system. pathogenicity relies on a type iii secretion system which delivers a large set of approximately 75 type iii effectors (t3e) into plant cells. on several plants, pathogenicity assays based on quantification of wilting symptoms failed to detect a significant contribution of r. solanacearum t3e in this process, thus revealing the collective ... | 2010 | 20687809 |
| production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in recombinant corynebacterium glutamicum using propionate as a precursor. | lipopolysaccharides free p[3-hydroxybutyrate (3hb)-co-3-hydroxyvalerate (3hv)] production was achieved using recombinant corynebacterium glutamicum harboring polyhydroxyalkanoate (pha) biosynthetic genes from ralstonia eutropha. cells grown on glucose with feeding of propionate as a precursor of 3hv unit accumulated 8-47wt% of p(3hb-co-3hv). the 3hv fraction in the copolymer was varied from 0 to 28mol% depending on the propionate concentrations. | 2011 | 20692303 |
| sulfoacetate is degraded via a novel pathway involving sulfoacetyl-coa and sulfoacetaldehyde in cupriavidus necator h16. | bacterial degradation of sulfoacetate, a widespread natural product, proceeds via sulfoacetaldehyde and requires a considerable initial energy input. whereas the fate of sulfoacetaldehyde in cupriavidus necator (ralstonia eutropha) h16 is known, the pathway from sulfoacetate to sulfoacetaldehyde is not. the genome sequence of the organism enabled us to hypothesize that the inducible pathway, which initiates sau (sulfoacetate utilization), involved a four-gene cluster (saurstu; h16_a2746 to h16_a ... | 2010 | 20693281 |
| nocapyrones a-d, gamma-pyrones from a nocardiopsis strain isolated from the marine sponge halichondria panicea. | four new gamma-pyrones, nocapyrones a-d (1-4), were isolated from an organic extract of the nocardiopsis strain hb383, which was isolated from the marine sponge halichondria panicea. these are the first gamma-pyrones reported from a nocardiopsis strain. the structures were elucidated on the basis of one- and two-dimensional nmr experiments and supported by hplc-uv/ms and hresims analyses. the biosynthesis of nocapyrone a was investigated by feeding experiments with (13)c-labeled compounds. in ad ... | 2010 | 20695474 |
| genome-wide sequencing data reveals virulence factors implicated in banana xanthomonas wilt. | banana xanthomonas wilt is a newly emerging disease that is currently threatening the livelihoods of millions of farmers in east africa. the causative agent is xanthomonas campestris pathovar musacearum (xcm), but previous work suggests that this pathogen is much more closely related to species xanthomonas vasicola than to x. campestris. we have generated draft genome sequences for a banana-pathogenic strain of xcm isolated in uganda and for a very closely related strain of x. vasicola pathovar ... | 2010 | 20695894 |
| ectopic expression of an ear motif deletion mutant of slerf3 enhances tolerance to salt stress and ralstonia solanacearum in tomato. | ethylene-responsive transcription factors (erfs) bind specifically to cis-acting dna regulatory elements such as gcc boxes and play an important role in the regulation of defense- and stress-related genes in plants. in contrast to other erfs, class ii erfs contain an erf-associated amphiphilic repression (ear) domain and act as gcc-mediated transcriptional repressors. in this study, slerf3, a class ii erf was isolated from tomato and characterized. to examine whether the ear motif of class ii er ... | 2010 | 20697739 |
| production of p(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate) terpolymers using a chimeric pha synthase in recombinant ralstonia eutropha and pseudomonas putida. | recombinant strains of ralstonia eutropha and pseudomonas putida harboring a chimeric polyhydroxyalkanoate (pha) synthase, which consisted of pha synthases of aeromonas caviae and r. eutropha, produced 3-hydroxybutyrate (3hb)-based pha copolymers comprised of 3-hydroxyhexanoate and 3-hydroxyoctanoate units from dodecanoate (87-97 mol % 3hb), indicating that the chimeric pha synthase possesses desirable substrate specificity leading to the production of 3hb-rich copolymers. | 2010 | 20699558 |
| bacteria from drinking water supply and their fate in gastrointestinal tracts of germ-free mice: a phylogenetic comparison study. | microorganisms in drinking water sources may colonize in gastrointestinal (gi) tracts and this phenomenon may pose a potential health risk especially to immunocompromised population. the microbial community diversity of the drinking water was compared with the gi tracts of the mice using phylogenetic and statistical analyses of 16s rrna gene sequences. a group of germ-free mice were fed with drinking water from public water supply that passed through an automated watering system with documented ... | 2010 | 20705313 |
| elucidation of beta-oxidation pathways in ralstonia eutropha h16 by examination of global gene expression. | ralstonia eutropha h16 is capable of growth and polyhydroxyalkanoate production on plant oils and fatty acids. however, little is known about the triacylglycerol and fatty acid degradation pathways of this bacterium. we compare whole-cell gene expression levels of r. eutropha h16 during growth and polyhydroxyalkanoate production on trioleate and fructose. trioleate is a triacylglycerol that serves as a model for plant oils. among the genes of note, two potential fatty acid β-oxidation operons an ... | 2010 | 20709892 |
| complex function by design using spatially pre-structured synthetic microbial communities: degradation of pentachlorophenol in the presence of hg(ii). | naturally occurring microbes perform a variety of useful functions, with more complex processes requiring multiple functions performed by communities of multiple microbes. synthetic biology via genetic engineering may be used to achieve desired multiple functions, e.g. multistep chemical and biological transformations, by adding genes to a single organism, but this is sometimes not possible due to incompatible metabolic requirements or not desirable in certain applications, especially in medical ... | 2011 | 20717565 |
| ralstonia solanacearum δpgi-1 strain kzr-5 is affected in growth, response to cold stress and invasion of tomato. | the survival and persistence of ralstonia solanacearum biovar 2 in temperate climates is still poorly understood. to assess whether genomic variants of the organism show adaptation to local conditions, we compared the behaviour of environmental strain kzr-5, which underwent a deletion of the 17.6 kb genomic island pgi-1, with that of environmental strain kzr-1 and potato-derived strains 1609 and 715. pgi-1 harbours two genes of potential ecological relevance, i.e. one encoding a hypothetical pro ... | 2011 | 20717661 |
| roles of multiple acetoacetyl coenzyme a reductases in polyhydroxybutyrate biosynthesis in ralstonia eutropha h16. | the bacterium ralstonia eutropha h16 synthesizes polyhydroxybutyrate (phb) from acetyl coenzyme a (acetyl-coa) through reactions catalyzed by a β-ketothiolase (phaa), an acetoacetyl-coa reductase (phab), and a polyhydroxyalkanoate synthase (phac). an operon of three genes encoding these enzymatic steps was discovered in r. eutropha and has been well studied. sequencing and analysis of the r. eutropha genome revealed putative isologs for each of the phb biosynthetic genes, many of which had never ... | 2010 | 20729355 |
| bacterial wilt resistance in tomato, pepper, and eggplant: genetic resources respond to diverse strains in the ralstonia solanacearum species complex. | bacterial wilt, caused by strains belonging to the ralstonia solanacearum species complex, inflicts severe economic losses in many crops worldwide. host resistance remains the most effective control strategy against this disease. however, wilt resistance is often overcome due to the considerable variation among pathogen strains. to help breeders circumvent this problem, we assembled a worldwide collection of 30 accessions of tomato, eggplant and pepper (core-tep), most of which are commonly used ... | 2011 | 20795852 |
| the oxygen-tolerant hydrogenase i from aquifex aeolicus weakly interacts with carbon monoxide: an electrochemical and time-resolved ftir study. | the [nife] hydrogenase (hase i) involved in the aerobic respiration of the hyperthermophilic bacterium aquifex aeolicus shows increased oxygen tolerance and thermostability and can form very stable films on pyrolytic graphite electrodes. oxygen-tolerant enzymes, like the ones from a. aeolicus and ralstonia eutropha, are reported to be insensitive to co inhibition. this is in contrast to known and well-characterized (oxygen-sensitive) hydrogenases, for which carbon monoxide is a competitive inhib ... | 2010 | 20815411 |
| specific responses of salmonella enterica to tomato varieties and fruit ripeness identified by in vivo expression technology. | recent outbreaks of vegetable-associated gastroenteritis suggest that enteric pathogens colonize, multiply and persist in plants for extended periods of time, eventually infecting people. genetic and physiological pathways, by which enterics colonize plants, are still poorly understood. | 2010 | 20824208 |
| dihydrolipoamide dehydrogenases of advenella mimigardefordensis and ralstonia eutropha catalyze cleavage of 3,3'-dithiodipropionic acid into 3-mercaptopropionic acid. | the catabolism of the disulfide 3,3'-dithiodipropionic acid (dtdp) is initiated by the reduction of its disulfide bond. three independent tn5::mob-induced mutants of advenella mimigardefordensis strain dpn7(t) were isolated that had lost the ability to utilize dtdp as the sole source of carbon and energy and that harbored the transposon insertions in three different sites of the same dihydrolipoamide dehydrogenase gene encoding the e3 subunit of the pyruvate dehydrogenase multi-enzyme complex of ... | 2010 | 20833784 |
| moderate temperature fluctuations rapidly reduce the viability of ralstonia solanacearum race 3, biovar 2, in infected geranium, tomato, and potato plants. | most ralstonia solanacearum strains are tropical plant pathogens, but race 3, biovar 2 (r3bv2), strains can cause bacterial wilt in temperate zones or tropical highlands where other strains cannot. r3bv2 is a quarantine pathogen in north america and europe because of its potential to damage the potato industry in cooler climates. however, r3bv2 will not become established if it cannot survive temperate winters. previous experiments showed that in water at 4°c, r3bv2 does not survive as long as n ... | 2010 | 20851983 |
| probing the active site of an o2-tolerant nad+-reducing [nife]-hydrogenase from ralstonia eutropha h16 by in situ epr and ftir spectroscopy. | | 2010 | 20857465 |
| ralstonia solanacearum dps contributes to oxidative stress tolerance and to colonization of and virulence on tomato plants. | ralstonia solanacearum, an economically important soilborne plant pathogen, infects host roots to cause bacterial wilt disease. however, little is known about this pathogen's behavior in the rhizosphere and early in pathogenesis. in response to root exudates from tomato, r. solanacearum strain uw551 upregulated a gene resembling dps, a nonspecific dna binding protein from starved cells that is critical for stress survival in other bacteria. an r. solanacearum dps mutant had increased hydrogen pe ... | 2010 | 20870795 |
| characterization of acrylamidase isolated from a newly isolated acrylamide-utilizing bacterium, ralstonia eutropha aum-01. | a mesophilic bacterium capable of utilizing acrylamide was isolated, aum-01, from soil collected from leaf litter at picnic point on the uw-madison campus. in minimal medium with acrylamide as the sole carbon and nitrogen source, a batch culture of aum-01 completely converted 28.0 mm acrylamide to acrylic acid in 8 h and reached a cell density of 0.3 (a₆₀₀)). afterward all the acrylic acid was degraded by 20 h with the cell density increasing to 1.9 (a₆₀₀). the acrylamide-utilizing bacterium was ... | 2010 | 20872004 |
| multiple lateral gene transfers and duplications have promoted plant parasitism ability in nematodes. | lateral gene transfer from prokaryotes to animals is poorly understood, and the scarce documented examples generally concern genes of uncharacterized role in the receiver organism. in contrast, in plant-parasitic nematodes, several genes, usually not found in animals and similar to bacterial homologs, play essential roles for successful parasitism. many of these encode plant cell wall-degrading enzymes that constitute an unprecedented arsenal in animals in terms of both abundance and diversity. ... | 2010 | 20876108 |
| bacterial community diversity in the brazilian atlantic forest soils. | the aim of this study was to characterize the bacterial community diversity of the brazilian atlantic forest soil by means of both cultivation and 16s rrna clone libraries. a collection of 86 representative isolates, obtained from six samples of atlantic forest soils from the national park of serra dos órgãos (parnaso), belonged to the genera arthrobacter, bacillus, burkholderia, leifsonia, paenibacillus, pseudomonas, ralstonia, serratia, and streptomyces according to the 16s rrna sequences. rep ... | 2010 | 20886336 |
| a new protein of alpha-amylase activity from lactococcus lactis. | an extracellular alpha-amylase from lactococcus lactis ibb500 was purified and characterized. the optimum conditions for the enzyme activity were ph 4.5, temperature of 35 degrees c, enzyme molecular mass of 121 kda. the genome analysis and a plasmid curing experiment indicated that amy+ genes were located in a plasmid of 30 kb. an analysis of phylogenetic relationships strongly supported a hypothesis of horizontal gene transfer. a strong homology was found for the peptides with the sequence of ... | 2010 | 20890096 |
| gas pressure effects on the rates of catalytic h(2) oxidation by hydrogenases. | during catalysis by hydrogenases, entities no larger than h(2) or h(+) reach and leave a deeply buried active site, by as yet unidentified pathways. novel experiments, conducted mainly with the membrane-bound [nife]-hydrogenase from ralstonia eutropha, explore why small excess gas pressures (h(2) or he) attenuate the rate of h(2) oxidation. | 2010 | 20922264 |
| complex prokaryotic genome structure: rapid evolution of chromosome ii. | although many bacteria with two chromosomes have been sequenced, the roles of such complex genome structuring are still unclear. to uncover levels of chromosome i (ci) and chromosome ii (cii) sequence divergence, mauve 2.2.0 was used to align the ci- and cii-specific sequences of bacteria with complex genome structuring in two sets of comparisons: the first set was conducted among the ci and cii of bacterial strains of the same species, while the second set was conducted among the ci and cii of ... | 2010 | 20924417 |
| investigations on the microbial catabolism of the organic sulfur compounds tdp and dtdp in ralstonia eutropha h16 employing dna microarrays. | in this study, we have investigated the transcriptome of ralstonia eutropha h16 during cultivation with gluconate in presence of 3,3'-thiodipropionic acid (tdp) or 3,3'-dithiodipropionic acid (dtdp) during biosynthesis of poly(3-hydroxybutyrate-co-3-mercaptopropionate). genome-wide transcriptome analyses revealed several genes which were upregulated during cultivation in presence of the above-mentioned compounds. obtained data strongly suggest that two abc-type transport system and three probabl ... | 2010 | 20924576 |
| phylogeny of fungal hemoglobins and expression analysis of the aspergillus oryzae flavohemoglobin gene fhba during hyphal growth. | the fhba genes encoding putative flavohemoglobins (fhb) from aspergillus niger and aspergillus oryzae were isolated. comparison of the deduced amino acid sequence of the a. niger fhba gene and other putative filamentous fungal fhb-encoding genes to that of ralstonia eutropha shows an overall conserved gene structure and completely conserved catalytic amino acids. several yeasts and filamentous fungi, including both aspergillus species have been found to contain a small fhb gene family mostly con ... | 2010 | 20960969 |
| resolvase-like serine recombinase mediates integration/excision in the bacteriophage φrsm. | ϕrsm1, a filamentous phage infecting ralstonia solanacearum, encodes an open reading frame (orf14) that exhibits significant homology to members of the resolvase/invertase subfamily of site-specific serine recombinases. similar prophages are found in the genomes of various strains of r. solanacearum, r. pickettii, and burkholderia pseudomallei. we have characterized the integrative and excisive recombination reactions mediated by the ϕrsm1 integrase using in vivo assays. an e. coli plasmid conta ... | 2010 | 21035394 |
| biosynthesis of polyhydroxyalkanoate homopolymers by pseudomonas putida. | pseudomonas putida kt2442 has been a well-studied producer of medium-chain-length (mcl) polyhydroxyalkanoate (pha) copolymers containing c6 ~ c14 monomer units. a mutant was constructed from p. putida kt2442 by deleting its phag gene encoding r-3-hydroxyacyl-acp-coa transacylase and several other β-oxidation related genes including fadb, fada, fadb2x, and fadax. this mutant termed p. putida kthh03 synthesized mcl homopolymers including poly(3-hydroxyhexanoate) (phhx) and poly(3-hydroxyheptanoate ... | 2010 | 21046374 |
| characterization of filamentous bacteriophage pe226 infecting ralstonia solanacearum strains. | the aim of this study was to isolate and characterize new bacteriophages that infect a wide range of plant pathogenic ralstonia solanacearum strains. | 2010 | 21054700 |
| growth performance and carcase quality in broiler chickens fed on bacterial protein grown on natural gas. | 1. the effects of increasing concentrations (0, 40, 80 or 120 g/kg) of bacterial protein meal (bpm) and bacterial protein autolysate (bpa) grown on natural gas on growth performance and carcase quality in broiler chickens were examined. 2. adding bpm to diets reduced feed intake and improved gain: feed from 0 to 21 d and overall to 35 d, but did not significantly affect weight gain compared to the soybean meal based control diet. 3. increasing concentrations of bpa significantly reduced growth r ... | 2010 | 21058073 |
| molecular assessment of microbiota structure and dynamics along mixed olive oil and winery wastewaters biotreatment. | the major parcel of the degradation occurring along wastewater biotreatments is performed either by the native microbiota or by added microbial inocula. the main aim of this study was to apply two fingerprinting methods, temperature gradient gel electrophoresis (tgge) and length heterogeneity-pcr (lh-pcr) analysis of 16s rrna gene fragments, in order to assess the microbiota structure and dynamics during mixed olive oil and winery wastewaters aerobic biotreatment performed in a jet-loop reactor ... | 2010 | 21069557 |
| in silico analysis reveals multiple putative type vi secretion systems and effector proteins in pseudomonas syringae pathovars. | type vi secretion systems (t6ss) of gram-negative bacteria form injectisomes that have the potential to translocate effector proteins into eukaryotic host cells. in silico analysis of the genomes in six pseudomonas syringae pathovars revealed that p. syringae pv. tomato dc3000, pv. tabaci atcc 11528, pv. tomato t1 and pv. oryzae 1-6 each carry two putative t6ss gene clusters (hsi-i and hsi-ii; hsi: hcp secretion island), whereas pv. phaseolicola 1448a and pv. syringae b728 each carry one. the pv ... | 2010 | 21091602 |
| differential occurrence of oxidative burst and antioxidative mechanism in compatible and incompatible interactions of solanum lycopersicum and ralstonia solanacearum. | striking increase in reactive oxygen species (ros) such as hydrogen peroxide (h(2)o(2)) has been demonstrated to occur in plants in response to pathogen attack. the aim of this study was to investigate the biochemical aspects of ros generation, antioxidative mechanism and cell wall reinforcement as responses of tomato cultivars arka meghali (am; susceptible) and bt-10 (bt; resistant) against ralstonia solanacearum (ralsol). while the oxidative burst was characterized by a single phase ros increa ... | 2010 | 21093281 |