| mineralization of biphenyl and pcbs by the white rot fungus phanerochaete chrysosporium. | the white rot fungus phanerochaete chrysosporium is unique in its ability to totally degrade a wide variety of recalcitrant pollutants. we have investigated the degradation of biphenyl and two model chlorinated biphenyls, 2,2',4,4'-tetrachlorobiphenyl and 2-chlorobiphenyl by suspended cultures of p. chrysosporium grown under conditions that maximize the synthesis of lignin-oxidizing enzymes. radiolabeled biphenyl and 2'-chlorobiphenyl added to cultures at concentrations in the range 260 nm to 8. ... | 1992 | 18601096 |
| gene silencing by rna interference in the white rot fungus phanerochaete chrysosporium. | the effectiveness of rna interference (rnai) is demonstrated in the lignin-degrading fungus phanerochaete chrysosporium. the manganese-containing superoxide dismutase gene (mnsod1) was used as the target for rnai. the plasmid constructed for gene silencing contained a transcriptional unit for hairpin rna expression. significantly lower mnsod expression at both the mrna and protein activity levels was detected in rnai transformants. furthermore, even though p. chrysosporium possesses three copies ... | 2008 | 18606804 |
| toward a control of lignin and manganese peroxidases hypersecretion by phanerochaete chrysosporium in agitated vessels: evidence of the superiority of pneumatic bioreactors on mechanically agitated bioreactors. | phanerochaete chrysosporium and cultivated both mechanically agitated and pneumatic bioreactors. in the pneumatic devices, the yields of lignin and manganese peroxidases as well as extracellular protein, were considerably increased as compared with mechanically agitated bioreactors. lignin peroxidase and manganese peroxidase activities as high as 4500 u x l(-1) and 1812 u x l(-1) respectively, were produced in an airlift bioreactor. by using enzyme markers, the secretion pathway and the respirat ... | 1993 | 18609572 |
| [kinetics model for batch culture of white rot fungus]. | in order to understand ligninolytic enzymes production process during culture of white rot fungus, accordingly to direct the design of fermentation process, a kinetics model was built for the batch culture of phanerochaete chrysosporium. the parameters in the model were calibrated based on the experimental data from free and immobilized culture separately. the difference between each variable's values calculated based on kinetics model and experimental data is within 15%. comparing parameters fo ... | 2008 | 18613526 |
| [white rot fungi biofilter treating waste gas containing chlorobenzene]. | a novel gas-solid phase bioreactor, using bamboo as support material, inoculated with white rot fungi phanerochaete chrysosporium was established to treat waste gas containing chlorobenzene. the performance of p. chrysosporium bioreactor was examined under different conditions. results showed that the maximum removal efficiency of nearly 80% (average removal efficiency of 50%) could be reached under the condition of chlorobenzene inlet concentration of 200-1500 mg/m3 and the empty bed retention ... | 2008 | 18613527 |
| production of lignin peroxidase by phanerochaete chrysosporium immobilized on porous poly(styrene-divinylbenzene) carrier and its application to the degrading of 2-chlorophenol. | porous poly(styrene-divinylbenzene) carriers, for the immobilization of white rot fungus phanerochaete chrysosporium have been prepared by the concentrated emulsion polymerization method. the concentrated emulsion consists of a mixture of styrene and divinylbenzene containing a suitable surfactant and an initiator as the continuous phase, and water as the dispersed phase. the polymerization of the monomers of the continuous phase generated the polymer carrier with a porcus structure. the white r ... | 1994 | 18618449 |
| process optimization and modeling of trichlorophenol degradation by phanerochaete chrysosporium. | the biodegradation of 2,4,6-trichlorophenol and 2,4,5-trichlorophenol by the white rot fungus phanerochaete chrysosporium was studied in batch and continuous reactor systems. experiments were conducted in shake flasks as well as in packed-bed reactors in which the fungus was immobilized. the degradation rates in the packed-bed reactors were found to be two orders of magnitude greater than those obtained in the shake flasks in which the fungus was just suspended. the degradation rate was found to ... | 1995 | 18623355 |
| immobilization of fungal spores by adhesion. | immobilization of conidiospores of phanerochaete chrysosporium by adhesion was investigated in static and flow conditions on flat and on porous supports. reducing the electrostatic repulsion between the spores and the support by adsorption of polycations on the support allows a better adhesion efficiency and a higher density of adhering spores and does not affect germination and growth. formation of spore aggregates either in the suspension (high ionic strength) or on the support tends to decrea ... | 1995 | 18623448 |
| effects of fungal pretreatment and steam explosion pretreatment on enzymatic saccharification of plant biomass. | the effects of consecutive treatments by a lignin-degrading fungus phanerochaete chrysosporium and by steam explosion for the enzymatic saccharification of plant biomass were studied experimentally, and the optimal operational conditions for obtaining the maximum saccharification were evaluated. beech wood-meal was treated by the fungus for 98 days and then by high steam temperatures of 170-230 degrees c with steaming times of 0-10 min. the treatment of the wood-meal by fungus prior to steam exp ... | 1995 | 18623542 |
| [evaluation of the damaging effect of dyestuff on phanerochaete chrysosporium with amplified fragment length polymorphism (aflp) analysis]. | the aflp analysis was carried out in order to evaluate the dna damaging effects of dyestuff of different concentrations on phanerochaete chrysosporium. the dna similarity and upgma cluster analysis were conducted to determine the relationships between the concentrations of dyestuff and dna damaging effects. the results showed that the aflp analysis could reflect the polymorphisms of dna well, and the primer pairs e-aac/m-caa could give more amplified dna fragments than others primers. from the d ... | 2008 | 18624206 |
| fungal secretomes--nature's toolbox for white biotechnology. | adapting their metabolism to varying carbon and nitrogen sources, saprophytic fungi produce an arsenal of extracellular enzymes, the secretome, which allows for an efficient degradation of lignocelluloses and further biopolymers. based on fundamental advances in electrophoretic, chromatographic, and mass spectrometric techniques on the one hand and the availability of annotated fungal genomes and sophisticated bioinformatic software tools on the other hand, a detailed analysis of fungal secretom ... | 2008 | 18636256 |
| continuous production of manganese peroxidase by phanerochaete chrysosporium immobilized on polyurethane foam in a pulsed packed-bed bioreactor. | the bottleneck of the application of manganese peroxidase (mnp) on an industrial scale in pulp biobleaching or in degradation of hazardous compounds is the lack of an efficient production system. three main problems arise for the continuous production of mnp during secondary metabolism of phanerochaete chrysosporium: enzyme production occurs only under specific physiological conditions corresponding to c or n limitation, high o(2) tension, and adequate mn(+2) concentration; the enzyme that is pr ... | 1997 | 18636618 |
| secretome analysis of phanerochaete chrysosporium strain cirm-brfm41 grown on softwood. | proteomic analysis was performed to determine and differentiate the composition of the secretomes of phanerochaete chrysosporium cirm-brfm41, a peroxidase hypersecretory strain grown under ligninolytic conditions and on softwood chips under biopulping conditions. extracellular proteins from both cultures were analyzed by bidimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry. a total of 37 spots were identified. the secretome in ... | 2008 | 18654772 |
| characterization of an endoglucanase belonging to a new subfamily of glycoside hydrolase family 45 of the basidiomycete phanerochaete chrysosporium. | the wood decay fungus phanerochaete chrysosporium has served as a model system for the study of lignocellulose conversions, but aspects of its cellulolytic system remain uncertain. here, we report identifying the gene that encodes the glycoside hydrolase (gh) family 45 endoglucanase (eg) from the fungus, cloning the cdna, determining its heterologous expression in the methylotrophic yeast pichia pastoris, and characterizing the recombinant protein. the cdna consisted of 718 bp, including an open ... | 2008 | 18676702 |
| degradation of lead-contaminated lignocellulosic waste by phanerochaete chrysosporium and the reduction of lead toxicity. | lead, as one of the most hazardous heavy metals to the environment interferes with lignocellulosic biomass bioconversion and carbon cycles in nature. the degradation of lead-polluted lignocellulosic waste and the restrain of lead hazards by solid-state fermentation with phanerochaete chrysosporium were studied. phanerochaete chrysosporium effectively degraded lignocellulose, formed humus and reduced active lead ions, even at the concentration of 400 mg/kg dry mass of lead. the highest lignocellu ... | 2008 | 18678031 |
| dutpase-containing metaviridae ltr retrotransposons from the genome of phanerochaete chrysosporium (fungi: basidiomycota). | | 2008 | 18680913 |
| growth and ligninolytic system production dynamics of the phanerochaete chrysosporium fungus a modelling and optimization approach. | the well-documented ability to degrade lignin and a variety of complex chemicals showed by the white-rot fungus phanerochaete chrysosporium has made it the subject of many studies in areas of environmental concern, including pulp bioleaching and bioremediation technologies. however, until now, most of the work in this field has been focused on the ligninolytic sub-system but, due to the great complexity of the involved processes, less progress has been made in understanding the biochemical regul ... | 2008 | 18694789 |
| bio-remediation of colored industrial wastewaters by the white-rot fungi phanerochaete chrysosporium and pleurotus ostreatus and their enzymes. | the effect of phanerochaete chrysosporium and pleurotus ostreatus whole cells and their ligninolytic enzymes on models of colored industrial wastewaters was evaluated. models of acid, direct and reactive dye wastewaters from textile industry have been defined on the basis of discharged amounts, economic relevance and representativeness of chemical structures of the contained dyes. phanerochaete chrysosporium provided an effective decolourization of direct dye wastewater model, reaching about 45% ... | 2009 | 18758969 |
| the white-rot fungus phanerochaete chrysosporium: conditions for the production of lignin-degrading enzymes. | investigating optimal conditions for lignin-degrading peroxidases production by phanerochaete chrysosporium (p. chrysosporium) has been a topic for numerous researches. the capability of p. chrysosporium for producing lignin peroxidases (lips) and manganese peroxidases (mnps) makes it a model organism of lignin-degrading enzymes production. focusing on compiling and identifying the factors that affect lip and mnp production by p. chrysosporium, this critical review summarized the main findings o ... | 2008 | 18810426 |
| [investigation of keratinophilic fungi in laodicea's recreation workshop]. | recreation ateliers in which archeological remnants are operated to be restorated to their original forms, are closed places with high humidity rates. the hands and the nails of archeology workers contact directly with the keratinophilic fungi during recreation facilities. the aim of this study was to investigate the presence and distribution of keratinophilic fungi in laodikeia antique city's recreation atelier (pamukkale region; aegean part of turkey). a total of 18 archeological remnant sampl ... | 2008 | 18822890 |
| effect of carbon and nitrogen source amendment on synthetic dyes decolourizing efficiency of white-rot fungus, phanerochaete chrysosporium. | decolourization activity of phanerochaete chrysosporium for three synthetic dyes viz., congo red, malachite green and crystal violet and impact of additional carbon and nitrogen supply on decolourization capacity of fungus were investigated. maximum decolourizing capacity was observed up to 15 ppm. addition of urea as nitrogen source and glucose as carbon source significantly enhanced decolourizing capacity (up to 87%) of fungus. in all the cases, both colour and cod were reduced more in non-ste ... | 2008 | 18831336 |
| incidence of keratinophilic fungi in waterlogged condition of paddy soil. | prevalence of keratinophilic fungi was observed in paddy field soil during different stages of cultivation viz., transplanting, tillering, milking and maturation. out of total 76 soil samples, 65 soil samples were found to be positive for the keratinophilic fungi. fourteen species belonging to a single genus chrysosporium were isolated through out the cropping season. c. keratinophilum (17.1%) followed by c. tropicum (13.15%) were found to be the most dominating geophilic species. the highest pe ... | 2008 | 18831344 |
| diversity analysis of soil dematiaceous hyphomycetes from the yellow river source area: i. | twenty-four soil samples of eight ecosystem-types around the yellow river source area were investigated for the number and specific composition of soil dematiaceous hyphomycetes by dilution plate technique. and then the co-relationship between genus species of soil dematiaceous hyphomycetes and ecosystem-types was analyzed. the results show that the amount and species distribution of soil dematiaceous hyphomycetes had an obvious variability in different ecosystem-types, and that the dominant gen ... | 2008 | 18837112 |
| differential expression in phanerochaete chrysosporium of membrane-associated proteins relevant to lignin degradation. | fungal lignin-degrading systems likely include membrane-associated proteins that participate in diverse processes such as uptake and oxidation of lignin fragments, production of ligninolytic secondary metabolites, and defense of the mycelium against ligninolytic oxidants. little is known about the nature or regulation of these membrane-associated components. we grew the white rot basidiomycete phanerochaete chrysosporium on cellulose or glucose as the carbon source and monitored the mineralizati ... | 2008 | 18849459 |
| expression and characterization of cyp51, the ancient sterol 14-demethylase activity for cytochromes p450 (cyp), in the white-rot fungus phanerochaete chrysosporium. | phanerochaete chrysosporium was the first fungal genome to exhibit more than one hundred cytochrome p450 (cyp) genes for a fungus within its genome (approximately 150). it can metabolize lignocellulose and a wide range of environmental xenobiotics including many carcinogens and pollutants where cytochromes p450 may be involved. in the present paper we describe the heterologous expression and characterization of an ancestral cyp form, sterol 14alpha-demethylase (cyp51-ec1.14.13.70), from this org ... | 2008 | 18853217 |
| two glucuronoyl esterases of phanerochaete chrysosporium. | the white-rot fungus phanerochaete chrysosporium produces glucuronoyl esterase, a recently discovered carbohydrate esterase, during growth on sugar beet pulp. two putative genes encoding this enzyme, ge1 and ge2, were isolated and cloned. heterologous expression in aspergillus vadensis, pycnoporus cinnabarinus and schizophyllum commune resulted in extracellular glucuronoyl esterase activity, demonstrating that these genes encode this enzymatic function. the amino acid sequence of ge1 was used to ... | 2009 | 18854978 |
| effects of culture media on larvicidal property of secondary metabolites of mosquito pathogenic fungus chrysosporium lobatum (moniliales: moniliaceae). | in order to determine the potential role of secondary metabolites of chrysosporium lobatum as a biological control agent for mosquitoes, effects of culture media on the larvicidal property of secondary metabolites was evaluated. the secondary metabolites of c. lobatum released in the sabouraud's dextrose broth (sdb) and chitin broth (cb) were collected by filtering through whatman no. 3 chr filter after 7 days of growth. first, second and third instars of anopheles stephensi and culex quinquefas ... | 2009 | 18951866 |
| expression of genes encoding laccase and manganese-dependent peroxidase in the fungus ceriporiopsis subvermispora is mediated by an ace1-like copper-fist transcription factor. | the effect of copper on the expression of genes encoding the ligninolytic enzymes laccase (lcs) and manganese peroxidase (mnp) in ceriporiopsis subvermispora was evaluated. this metal increased transcript levels of lcs, mnp1 and mnp2. this finding was not unexpected in the case of lcs, since its promoter contains a putative ace element. originally characterized in the yeast saccharomyces cerevisiae, ace is the target sequence of the ace1 copper-responsive transcription factor in this microorgani ... | 2009 | 18955150 |
| biodegradation of crosslinked acrylic polymers by a white-rot fungus. | two synthetic superabsorbent crosslinked acrylic polymers were mineralized by the white-rot fungus phanerochaete chrysosporium. the amount of polymer converted to co(2) increased as the amount of polymer added to the cultures increased. in the presence of sufficiently large amounts of the superabsorbents, such that all of the culture fluid was absorbed and a gelatinous matrix was formed, the fungus still grew and mineralization was observed. neither the polymers, nor their degradation products w ... | 1997 | 19002412 |
| biodegradation of superabsorbent polymers in soil. | biodegradation of two superabsorbent polymers, a crosslinked, insoluble polyacrylate and an insoluble polyacrylate/ polyacrylamide copolymer, in soil by the white-rot fungus, phanerochaete chrysosporium was investigated. the polymers were both solubilized and mineralized by the fungus but solubilization and mineralization of the copolymer was much more rapid than of the polyacrylate. soil microbes poorly solublized the polymers and were unable to mineralize either intact polymer. however, soil m ... | 2000 | 19009427 |
| sensitive detection of lip genes by electrochemical dna sensor and its application in polymerase chain reaction amplicons from phanerochaete chrysosporium. | an electrochemical dna sensor based on the sandwich hybridization recognition of target sequence of lignin peroxidase (lip) genes on a gold electrode was developed. a monolayer of thiolated capture probe was formed on a gold electrode through self-assembling. following hybridizations with target nucleic acid and biotinylated detection probe, streptavidin-horseradish peroxidase (hrp) conjugate was applied to the electrode. the dna conformation and surface coverage on electrode were characterized ... | 2009 | 19010661 |
| quality evaluation of co-composted wheat straw, poultry droppings and oil seed cakes. | poultry droppings, neem cake, castor cake, jatropha cake and grass clippings were used separately as organic nitrogen additives to decrease the high c:n ratio of wheat straw. composting was carried out aerobically in presence of fungal consortium developed by including aspergillus awamori, aspergillus nidulans, trichoderma viride and phanerochaete chrysosporium. the degraded product was characterized to assess the technical viability of organic nitrogen supplements as well as fungal consortium i ... | 2009 | 19015937 |
| fungal biodegradation of hard coal by a newly reported isolate, neosartorya fischeri. | cynodon dactylon (bermuda grass) has been observed to grow sporadically on the surface of coal dumps in the witbank coal mining area of south africa. root zone investigation indicated that a number of fungal species may be actively involved in the biodegradation of hard coal, thus enabling the survival of the plant, through mutualistic interaction, in this extreme environment. in an extensive screening program of over two thousand samples, the deuteromycete, neosartorya fischeri, was isolated an ... | 2008 | 19016511 |
| genetic analysis of protoplast fusant xhhh constructed for pharmaceutical wastewater treatment. | in order to analyse genetic relationships between functional strain xhhh previously constructed through protoplast fusion for pharmaceutical wastewater treatment and its parents, random amplification polymorphic dna (rapd) and polymerase chain reaction (pcr) were used to investigate genetic similarities among the strains based on genome and functional genes analyses. a total of 739 clear and consistent bands were produced in the rapd fingerprint analysis with 40 primers. the genetic similarity i ... | 2009 | 19027290 |
| site-directed mutagenesis of manganese peroxidase from phanerochaete chrysosporium in an in vitro expression system. | an enzymatically active fungal manganese peroxidase (mnp) from phanerochaete chrysosporium was synthesized in an in vitro coupled transcription-translation system. the synthesized mnp had the expected molecular mass (43,000da) and catalyzed the oxidation of 2,6-dimethoxyphenol (dmp) in the presence of hydrogen peroxide and mn(ii). a distal arginine residue (arg 42) of the peroxide binding pocket and the potential n-glycosylation site (asn 131) was site-directed mutagenized and corresponding muta ... | 2009 | 19027802 |
| characteristics and function of a low-molecular-weight compound with reductive activity from phanerochaetechrysosporium in lignin biodegradation. | a novel reductive compound with molecular weight of about 1000da, named pc reducer, was purified from the liquid culture of a white-rot basidiomycete phanerochaetechrysosporium. it was likely to have an alkene-ester structure according to fourier-transform infrared spectroscopy (ft-ir) and nuclear magnetic resonance (nmr) spectra. pc reducer reduced the hydroxyl radical ho(*) and the stable nitroxide radical under certain conditions. it inhibited the repolymerization of the products from the oxi ... | 2009 | 19038543 |
| ability of white-rot fungi to remove selected pharmaceuticals and identification of degradation products of ibuprofen by trametes versicolor. | a screening using four white-rot fungi (trametes versicolor, irpex lacteus, ganoderma lucidum and phanerochaete chrysosporium) was performed on the degradation of 10 mg l(-1) of ibuprofen (ibu, anti-inflammatory), clofibric acid (clofi, lipid regulator) and carbamazepine (carba, antiepileptic/analgetic) after 7 d of incubation. whereas ibu was extensively degraded by all the fungi tested, t. versicolor was the only strain able to degrade either clofi (approximately 91%) and carba (approximately ... | 2009 | 19062071 |
| biodegradation of phenolic compounds from coking wastewater by immobilized white rot fungus phanerochaete chrysosporium. | a white rot fungus phanerochaete chrysosporium, immobilized with the wood chips of italian poplar, was employed for biodegradation of phenolic compounds in coking wastewater. the immobilized fungus, dried by vacuum freeze desiccator, was kept high activity after a 9-month preservation and easy to be activated and domesticated. the removal rates of phenolic compounds and cod by immobilized fungus were 87.05% and 72.09% in 6 days, which were obviously higher than that by free fungus. for phenolic ... | 2009 | 19062164 |
| atypical biosynthetic properties of a delta 12/nu+3 desaturase from the model basidiomycete phanerochaete chrysosporium. | the model white-rot basidiomycete phanerochaete chrysosporium contains a single integral membrane delta(12)-desaturase fad2 related to the endoplasmic reticular plant fad2 enzymes. the fungal fad2-like gene was cloned and distinguished itself from plant homologs by the presence of four introns and a significantly larger coding region. the coding sequence exhibits ca. 35% sequence identity to plant homologs, with the highest sequence conservation found in the putative catalytic and major structur ... | 2009 | 19088315 |
| limitations of a membrane gradostat bioreactor designed for enzyme production from biofilms of phanerochaete chrysosporium. | growing interest has been shown in the continuous production of high-value products such as extracellular secondary metabolites used in the biotechnology, bioremediation and pharmaceutical industries. these high-value extracellular secondary metabolites are mostly produced in submerged fermentations. however, the use of continuous membrane bioreactors was determined to be highly productive. a novel membrane bioreactor, classified as a membrane gradostat reactor (mgr) was developed to immobilize ... | 2008 | 19092204 |
| cellobiose dehydrogenase-dependent biodegradation of polyacrylate polymers by phanerochaete chrysosporium. | when phanerochaete chrysosporium was cultured using conditions which promote the expression of cellobiose dehydrogenase (cdh), but not the ligninolytic peroxidases, the fungus effectively solubilized and mineralized an insoluble, crosslinked polyacrylate and an insoluble polyacrylate/polyacrylamide copolymer. addition of iron to the cultures increased cdh activity in the cultures and the rate and extent of solubilization and mineralization of both polymers. solubilization of both polymers was ob ... | 2000 | 19104874 |
| isolation and characterization of a new fungal species, chrysosporium ophiodiicola, from a mycotic granuloma of a black rat snake (elaphe obsoleta obsoleta). | isolation and characterization of the new species chrysosporium ophiodiicola from a mycotic granuloma of a black rat snake (elaphe obsoleta obsoleta) are reported. analysis of the sequences of different fragments of the ribosomal genes demonstrated that this species belongs to the onygenales and that this species is genetically different from other morphologically similar species of chrysosporium. this new species is unique in having both narrow and cylindrical-to-slightly clavate conidia and a ... | 2009 | 19109465 |
| enhanced transformation of malachite green by laccase of ganoderma lucidum in the presence of natural phenolic compounds. | in this study, we investigated the efficacy of phenolic extract of wheat bran and lignin-related phenolic compounds as natural redox mediators on laccase-mediated transformation of malachite green (mg) using purified laccase from the white-rot fungus ganoderma lucidum. g. lucidum laccase was able to decolorize 40.7% mg dye (at 25 mg l(-1)) after 24 h of incubation. whereas, the addition of phenolic extract of wheat bran enhanced the decolorization significantly (p<0.001) by two- to threefold tha ... | 2009 | 19130052 |
| nature and bioactivities of endolichenic fungi in pseudocyphellaria sp., parmotrema sp. and usnea sp. at hakgala montane forest in sri lanka. | the aim of this study was to investigate the nature and bioactivities of endolichenic fungi in three abundant lichens, pseudocyphellaria sp., usnea sp. and parmotrema sp. in the lower elevation of hakgala montane forest in sri lanka. | 2009 | 19141037 |
| biosorption of lead, copper, and cadmium by phanerochaete chrysosporium in ternary metal mixtures: statistical analysis of individual and interaction effects. | biosorption of three divalent metals, viz., lead, copper, and cadmium in ternary aqueous mixtures was studied using phanerochaete chrysosporium in batch shake flasks. the mixtures were prepared containing the metals at their either varying optimum or equal initial concentration combinations in aqueous solution of ph optimum to each of the metals. following were the optimum initial concentration ranges of the metals in mixture: lead, 60-100 mg/l; copper, 20-60 mg/l; and cadmium, 5-15 mg/l. and, f ... | 2009 | 19156371 |
| effect of phanerochaete chrysosporium inoculation during maturation of co-composted agricultural wastes mixed with olive mill wastewater. | the co-composting of olive mill wastewater with a variety of agricultural wastes was investigated. to reduce the toxicity of the phenolic fraction and to improve the degree of maturity of the compost, inoculation with the white-rot fungus phanerochaete chrysosporium was carried out during the maturation phase. the results showed that agricultural wastes that contain high levels of lignin-related compounds, such as the residue from trimmings, improved the microbial activity and thus reduced the s ... | 2009 | 19157833 |
| caryophyllenes from a fungal culture of chrysosporium pilosum. | four new caryophyllene derivatives, sch 725432 (1), sch 601253 (2), sch 601254 (3), and sch 725434 (4), were isolated from the fungal fermentation broth of chrysosporium pilosum by reversed-phase hplc purification. the structure elucidation of trioxygenated caryophyllenes 1-4 was accomplished on the basis of spectroscopic data interpretation. sch 725434 (4) possesses a dihydrofuran-3-one ring, forming a tricyclic ring skeleton, which represents an unprecedented ring skeleton for the caryophyllen ... | 2009 | 19183048 |
| crystal structure of glycoside hydrolase family 55 {beta}-1,3-glucanase from the basidiomycete phanerochaete chrysosporium. | glycoside hydrolase family 55 consists of beta-1,3-glucanases mainly from filamentous fungi. a beta-1,3-glucanase (lam55a) from the basidiomycete phanerochaete chrysosporium hydrolyzes beta-1,3-glucans in the exo-mode with inversion of anomeric configuration and produces gentiobiose in addition to glucose from beta-1,3/1,6-glucans. here we report the crystal structure of lam55a, establishing the three-dimensional structure of a member of glycoside hydrolase 55 for the first time. lam55a has two ... | 2009 | 19193645 |
| genome, transcriptome, and secretome analysis of wood decay fungus postia placenta supports unique mechanisms of lignocellulose conversion. | brown-rot fungi such as postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood. systematic examination of the p. placenta genome, transcriptome, and secretome revealed unique extracellular enzyme systems, including an unusual repertoire of extracellular glyco ... | 2009 | 19193860 |
| antagonism of microsporum species by soil fungi. | eighteen fungi isolated from soil by hair bating method were tested against soil inhabiting microsporum equinum, microsporum fulvum, microsporum gypseum and microsporum racemosum for their antagonistic interactions. colony inhibition during dual cultures showed inhibition of all the four microsporum species. the maximum inhibition of m. equinum, m. fulvum, m. gypseum and m. racemosum was caused by chrysosporium keratinophilum, chrysosporium tropicum, curvularia lunata and chrysosporium lucknowen ... | 2010 | 19207833 |
| degradation of tetracycline and oxytetracycline by crude lignin peroxidase prepared from phanerochaete chrysosporium--a white rot fungus. | pharmaceuticals are becoming an emerging environmental issue that attracts increasing attention in recent years. this study chose tetracycline (tc) and oxytetracycline (otc) as examples of pharmaceuticals. crude lignin peroxidase (lip) produced by phanerochaete chrysosporium were used to degrade tc and otc in vitro. the results illustrated that lip has a strong degrading ability towards tc and otc. at 50 mgl(-1) of tc and otc and 40 ul(-1) of the enzyme activity, the degradation of tc and otc re ... | 2009 | 19232429 |
| [degradation of soil phenolic acids by phanerochaete chrysosporium under continuous cropping of cucumber]. | with inoculation of phanerochaete chrysosporium in shaking flask, this paper studied the degradation of soil p-hydroxybenzoic acid, vanillic acid and ferulic acid under continuous cropping of cucumber, and evaluated the effect of this inoculation in overcoming the continuous cropping obstacle of cucumber. the results showed that after 8 days of inoculation, more than 99% of soil phenolic acids were decomposed by p. chrysosporium. compared with the control, the contents of soil phenolic acids und ... | 2008 | 19238850 |
| ligninase-mediated removal of natural and synthetic estrogens from water: i. reaction behaviors. | our experiments revealed that a few natural and synthetic estrogens can be effectively transformed through reactions that are mediated by lignin peroxidase (lip), an extracellular enzyme that is produced by a white rot fungus phanerochaete chrysosporium and is widely present in the natural environment we systematically assessed the reaction efficiencies at varying important conditions and identified the reaction products using mass spectrometry. in particular, we compared the reaction behaviors ... | 2009 | 19238967 |
| biodegradation of endocrine-disrupting compounds and suppression of estrogenic activity by ligninolytic fungi. | endocrine-disrupting compounds (edcs) represent a large group of substances of natural and anthropogenic origin. they are widely distributed in the environment and can pose serious risks to aquatic organisms and to public health. in this study, 4-n-nonylphenol, technical 4-nonylphenol, bisphenol a, 17alpha-ethinylestradiol, and triclosan were biodegraded by eight ligninolytic fungal strains (irpex lacteus 617/93, bjerkandera adusta 606/93, phanerochaete chrysosporium me 446, phanerochaete magnol ... | 2009 | 19243809 |
| molecular diagnosis of disseminated adiaspiromycosis due to emmonsia crescens. | emmonsia crescens is a saprophytic fungus that is distributed worldwide, causing diseases mostly in rodents. it has also been described, though rarely, as an etiologic agent of pulmonary pathology in humans, potentially leading to death. a case of pulmonary adiaspiromycosis is reported in a 30-year-old immunocompetent man. the patient presented with a history of several weeks of weakness, cough, fever, and weight loss of 10 kg. clinical and radiographic findings showed pulmonary lesions consiste ... | 2009 | 19244459 |
| [research and application of producing laccase by phanerochaete chrysosporium in solid-state fermentation system]. | the potential of banana skin and corn cob as a support-substrate for the production of extracellular laccase by the white-rot fungus phanerochaete chrysosporium (bkmf-1767) was investigated. the results indicate that laccase showed a maximum activity of 12.68 u/g when the proportion of banana skin and corn cob is 1:2 and the inducer is 0.4 mmol/l cuso4. in addition, crude laccase enzyme shows degradation activity to pentachlorophenol (pcp) without redox mediator or with the redox mediator (abts) ... | 2008 | 19256402 |
| optimization of lignin peroxidase production and stability by phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor. | a laboratory-scale study was carried out to produce lignin peroxidase (ligninase) by white rot fungus (phanerochaete chrysosporium) using sewage-treatment-plant (stp) sludge as the major substrate. the optimization was done using full-factorial design (ffd) with agitation and aeration as the two parameters. nine experiments indicated by the ffd were fermented in a stirred-tank bioreactor for 3 days. a second-order quadratic model was developed using the regression analysis of the experimental re ... | 2009 | 19259713 |
| decolouration of azo dyes by phanerochaete chrysosporium immobilised into alginate beads. | because of high discharged volumes and effluent composition, wastewater from the textile industry can be considered as the most polluting amongst all industrial sectors, thus greatly requiring appropriate treatment technologies. although some abiotic methods for the reduction of several dyes exist, these require highly expensive catalysts and reagents. biotechnological approaches were proven to be potentially effective in the treatment of this pollution source in an eco-efficient manner. the whi ... | 2010 | 19259719 |
| biodegradation of gaseous chlorobenzene by white-rot fungus phanerochaete chrysosporium. | to evaluate the effect of white rot fungus phanerochaete chrysosporium on removal of gaseous chlorobenzene. | 2008 | 19263802 |
| effect of bio-treatment on the lipophilic and hydrophilic extractives of wheat straw. | wheat straw, an important papermaking raw material in china, was treated with a white-rot fungus of phanerochaete chrysosporium me446, and the lipophilic and hydrophilic extractives from the control and bio-treated samples were analyzed by gc and gc-ms. bio-treatment of wheat straw could alter the chemical composition of both the lipophylic and hydrophilic extractives. sugars and phenolic substances such as coniferyl alcohol, 4-hydroxycinnamic acid, 1-guaiacylglycerol and ferulic acid were subst ... | 2009 | 19268580 |
| biological treatment of the effluent from a bleached kraft pulp mill using basidiomycete and zygomycete fungi. | three white-rot fungi (pleurotus sajor caju, trametes versicolor and phanerochaete chrysosporium) and one soft-rot fungi (rhizopus oryzae) species confirmed their potential for future applications in the biological treatment of effluents derived from the secondary treatment of a bleached kraft pulp mill processing eucalyptus globulus. among the four species p. sajor caju and r. oryzae were the most effective in the biodegradation of organic compounds present in the effluent, being responsible fo ... | 2009 | 19269018 |
| production of bioethanol by direct bioconversion of oil-palm industrial effluent in a stirred-tank bioreactor. | the purpose of this study was to evaluate the feasibility of producing bioethanol from palm-oil mill effluent generated by the oil-palm industries through direct bioconversion process. the bioethanol production was carried out through the treatment of compatible mixed cultures such as thrichoderma harzianum, phanerochaete chrysosporium, mucor hiemalis, and yeast, saccharomyces cerevisiae. simultaneous inoculation of t. harzianum and s. cerevisiae was found to be the mixed culture that yielded th ... | 2009 | 19294441 |
| acute conjunctivitis with episcleritis and anterior uveitis linked to adiaspiromycosis and freshwater sponges, amazon region, brazil, 2005. | we conducted an epidemiologic investigation of an outbreak of ocular disease among children to determine whether the disease was linked to emmonsia sp., a rarely-reported fungus and an agent of adiaspiromycosis. using an unmatched case-control study design, we compared case-patients with asymptomatic controls randomly selected from the population. scleral biopsies were analyzed microscopically. of 5,084 children examined, 99 case-patients were identified; mean age (+1 sd) was 11.0 +/- 4.4 years. ... | 2009 | 19331759 |
| increased pcp removal by amylomyces rouxii transformants with heterologous phanerochaete chrysosporium peroxidases supplementing their natural degradative pathway. | fungal peroxidases and phenoloxidases are widely used in aromatic toxic compounds degradation. peroxidases, such as lignin peroxidase and manganese peroxidase, as well as laccases are mainly produced by basidiomycetes and to a lower extent by other fungi, such as ascomycetes. peroxidase-encoding genes have been described and homologous expression has been achieved in basidiomycetes. heterologous expression has also been achieved in some non-producing peroxidase ascomycetes, like penicillium and ... | 2009 | 19340422 |
| induction of extracellular hydroxyl radical production by white-rot fungi through quinone redox cycling. | a simple strategy for the induction of extracellular hydroxyl radical (oh) production by white-rot fungi is presented. it involves the incubation of mycelium with quinones and fe(3+)-edta. succinctly, it is based on the establishment of a quinone redox cycle catalyzed by cell-bound dehydrogenase activities and the ligninolytic enzymes (laccase and peroxidases). the semiquinone intermediate produced by the ligninolytic enzymes drives oh production by a fenton reaction (h(2)o(2) + fe(2+) --> oh + ... | 2009 | 19376892 |
| transcriptome and secretome analyses of phanerochaete chrysosporium reveal complex patterns of gene expression. | the wood decay basidiomycete phanerochaete chrysosporium was grown under standard ligninolytic or cellulolytic conditions and subjected to whole-genome expression microarray analysis and liquid chromatography-tandem mass spectrometry of extracellular proteins. a total of 545 genes were flagged on the basis of significant changes in transcript accumulation and/or peptide sequences of the secreted proteins. under nitrogen or carbon limitation, lignin and manganese peroxidase expression increased r ... | 2009 | 19376920 |
| involvement of ligninolytic enzymes of phanerochaete chrysosporium in treating the textile effluent containing astrazon red fbl in a packed-bed bioreactor. | the effect of tween80, mn(ii) and veratryl alcohol (va) on the production of ligninolytic enzymes of phanerochaete chrysosporium in a packed-bed bioreactor using small pieces of kissiris as carrier, was investigated. the results of the enzyme activities were noticeable in terms of decolorization and cod removal of the textile effluent containing an azo dye (astrazon red fbl). no dilution was made on the tested textile effluent and it was not sterilized, also. maximum decolorization of the dye (8 ... | 2009 | 19395172 |
| the first genome-level transcriptome of the wood-degrading fungus phanerochaete chrysosporium grown on red oak. | as part of an effort to determine all the gene products involved in wood degradation, we have performed massively parallel pyrosequencing on an expression library from the white rot fungus phanerochaete chrysosporium grown in shallow stationary cultures with red oak as the carbon source. approximately 48,000 high quality sequence tags (246 bp average length) were generated. 53% of the sequence tags aligned to 4,262 p. chrysosporium gene models, and an additional 18.5% of the tags reliably aligne ... | 2009 | 19396602 |
| expression of lignin peroxidase h2 from phanerochaete chrysosporium by multi-copy recombinant pichia strain. | the liph2 gene, encoding the expression of lignin peroxidase, was cloned from phanerochaete chrysosporium bkm-f-1767 and expressed in pichia pastoris x-33, a yeast. the cdna of liph2 was generated from total rna extracted from p. chrysosporium by pcr with primers that do not contain a p. chrysosporium lignin peroxidase secretion signal. the gene was then successfully inserted into the expression vector ppiczalpha, and resulted in the recombinant vector ppiczalpha-liph2. the transformation was co ... | 2009 | 19402425 |
| microbial pretreatment of cotton stalks by submerged cultivation of phanerochaete chrysosporium. | this study used the fungus, phanerochaete chrysosporium, to pretreat cotton stalks with two methods, shallow stationary and agitated cultivation, at three supplemental salt concentrations. pretreatment efficiencies were compared by evaluating lignin degradation, solid recovery and carbohydrate availability over a 14-day period. shallow stationary cultivation with no salts gave 20.7% lignin degradation along with 76.3% solid recovery and 29.0% carbohydrate availability. the highest lignin degrada ... | 2009 | 19423334 |
| biodegradation of the high explosive hexanitrohexaazaiso-wurtzitane (cl-20). | the aerobic biodegradability of the high explosive cl-20 by activated sludge and the white rot fungus phanerochaete chrysosporium has been investigated. although activated sludge is not effective in degrading cl-20 directly, it can mineralize the alkaline hydrolysis products. phanerochaete chrysosporium degrades cl-20 in the presence of supplementary carbon and nitrogen sources. biodegradation studies were conducted using various nutrient media under diverse conditions. variables included the cl ... | 2009 | 19440524 |
| biodegradation aspects of polycyclic aromatic hydrocarbons (pahs): a review. | pahs are aromatic hydrocarbons with two or more fused benzene rings with natural as well as anthropogenic sources. they are widely distributed environmental contaminants that have detrimental biological effects, toxicity, mutagenecity and carcinogenicity. due to their ubiquitous occurrence, recalcitrance, bioaccumulation potential and carcinogenic activity, the pahs have gathered significant environmental concern. although pah may undergo adsorption, volatilization, photolysis, and chemical degr ... | 2009 | 19442441 |
| biological conversion of olive pomace into compost by using trichoderma harzianum and phanerochaete chrysosporium. | olive pomace was composted by using a reactor for a period of 50 days in four bioreactors. urea was added to adjust c/n ration between 25-30. at the end of 50 days of composting using trichoderma harzianum and phanerochaete chrysosporium, cellulose and lignin were highly degraded. it was found that after 30 days, p. chrysosporium and t. harzianum degraded approximately 71.9% of the lignin and 59.25% of the cellulose, respectively. the percent of ash content in the raw waste mixture was 13%. this ... | 2009 | 19467866 |
| extracellular proteomic analysis for degradation of pahs in source of drinking water with fusant strains. | extracellular proteomic expressions of two fusant strains were analyzed to observe their abilities to degrade polycyclic aromatic hydrocarbons (pahs) in the source of drinking water from yangtze river. the extracellular proteomes for the hybrid strains, fhhh and xhhh, and one of their parental strain phanerochaete chrysosporium were measured by the two-dimensional electrophoresis and ms/ms. the similarity of proteome expression was 34.7% between fhhh and p. chrysosporium and that was 28.6% betwe ... | 2009 | 19499333 |
| pyranose 2-oxidase from phanerochaete chrysosporium--expression in e. coli and biochemical characterization. | the presented work reports the isolation and heterologous expression of the p2ox gene encoding the flavoprotein pyranose 2-oxidase (p2ox) from the basidiomycete phanerochaete chrysosporium. the p2ox cdna was inserted into the bacterial expression vector pet21a(+) and successfully expressed in escherichia coli. we obtained active, fully flavinylated recombinant p2ox in yields of approximately 270 mg/l medium. the recombinant enzyme was provided with an n-terminal t7-tag and a c-terminal his(6)-ta ... | 2009 | 19501263 |
| treatment of vocs in biofilters inoculated with fungi and microbial consortium. | an experimental study on the removal of xylene vapours from an air stream was conducted on three identical upflow laboratory-scale wood-chips-based bed biofilters. three different inoculums were used: fungi (phanerochaete chrysosporium and cladosporium sphaerospermum), a bacterial consortium (evb110), and a mixed culture of fungi and evb 110. the empty bed gas residence time was 59 s, and various inlet concentrations of the contaminant were tested. the results obtained revealed a strong correlat ... | 2009 | 19507439 |
| adiaspiromycosis due to emmonsia crescens is widespread in native british mammals. | adiaspiromycosis caused by emmonsia crescens is primarily a respiratory disease affecting small mammals, especially members of the families rodentia, carnivora and mustelidae. although isolated reports exist of adiaspiromycosis in free-living british wildlife, the extent of infection in wild animals in the uk, and the significance of any associated pathology are unclear. here, we report the results of histopathological examination of lungs of free-living wild mammals from the south-west uk coupl ... | 2009 | 19533414 |
| melanin synthesis by sclerotinia sclerotiorum. | we confirmed that the melanin produced by sclerotinia sclerotiorum is a dihydroxynaphthalene (dhn). the specific dhn melanogenesis inhibitor test that uses tricyclazole at low levels (typically 2-5 ppm) to cause a confirmatory appearance of soluble red-brown inhibition products does not work when analyzing melanin synthesis in the sclerotia of s. sclerotiorum. we demonstrated the presence of scytalone dehydratase, an enzyme specific to dhn melanogenesis, in melanized sclerotia and melanized nons ... | 2009 | 19537203 |
| role of p450 monooxygenases in the degradation of the endocrine-disrupting chemical nonylphenol by the white rot fungus phanerochaete chrysosporium. | the white rot fungus phanerochaete chrysosporium extensively degraded the endocrine disruptor chemical nonylphenol (np; 100% of 100 ppm) in both nutrient-limited cultures and nutrient-sufficient cultures. the p450 enzyme inhibitor piperonyl butoxide caused significant inhibition (approximately 75%) of the degradation activity in nutrient-rich malt extract (me) cultures but no inhibition in defined low-nitrogen (ln) cultures, indicating an essential role of p450 monooxygenase(s) in np degradation ... | 2009 | 19542331 |
| survey of keratinophilic fungi in sewage sludge from wastewater treatment plants of mazandaran, islamic republic of iran. | to isolate keratinophilic fungi in sewage sludge from wastewater treatment plants in sari city, mazandaran province, islamic republic of iran, samples were taken from 7 plants with different sewage treatment technologies. from 35 sludge samples cultured on sabouraud's agar with cycloheximide and chloramphenicol, 326 fungal colonies belonging to 7 species were isolated. geotrichum (59.5%), cladosporium (13.8%), alternaria (11.3%) and penicillium (10.7%) species were the most prevalent. no growth ... | 2009 | 19554993 |
| gene expression analysis of phanerochaete chrysosporium during the transition time from primary growth to secondary metabolism. | in order to identify the secondary metabolism-related genes of phanerochaete chrysosporium growing under pure o2 and nitrogen-limited conditions, 2322 ests fragments originated from two suppression-subtractive libraries were analyzed using the cdna microarray technique. ten significantly upregulated and 22 significantly downregulated genes were identified in the 72 h cultured mycelia rna samples (secondary metabolism). according to qpcr, 16 out of the 32 genes were expressed differently in secon ... | 2009 | 19557348 |
| enzymatic properties of cytochrome p450 catalyzing 3'-hydroxylation of naringenin from the white-rot fungus phanerochaete chrysosporium. | we cloned full-length cdnas of more than 130 cytochrome p450s (p450s) derived from phanerochaete chrysosporium, and successfully expressed 70 isoforms using a co-expression system of p. chrysosporium p450 and yeast nadph-p450 reductase in saccharomyces cerevisiae. of these p450s, a microsomal p450 designated as pccyp65a2 consists of 626 amino acid residues with a molecular mass of 68.3kda. sequence alignment of pccyp65a2 and human cyp1a2 revealed a unique structure of pccyp65a2. functional analy ... | 2009 | 19576179 |
| dermatophytes, related keratinophilic and opportunistic fungi in indoor dust of houses and hospitals. | dermatophytes, related keratinophilic and opportunistic fungi were isolated from indoor dust samples of 46 hospitals and 47 houses in kanpur. a total of 19 fungi represented by 11 genera were isolated by the hair-baiting technique from 230 and 235 samples from hospitals and houses respectively. the isolated fungi are acremonium implicatum (indian type culture collection) itcc 5266, a. strictum (germplasm centre for keratinophilic fungi) gpck 1137, aphanoascus fulvescens gpck 1081, arthroderma si ... | 2009 | 19584506 |
| [molecular cloning and expression of alcohol dehydrogenase gene of phanerochaete chrysosporium]. | when phanerochaete chrysosporium is grown under oxygen-limited condition, ethanol is one of the important metabolites. in order to understand the metabolic mechanism of p. chrysosporium grown under oxygen-limited condition, a cdna sequence (1 071 bp) designated "pcadh1" encoding an alcohol dehydrogenase (adh) was cloned from the filamentous white-rot fungus p. chrysosporium. pcadh1 gene encodes a protein of 356 amino acid residues. although the catalytic domain and coenzyme-binding domain were h ... | 2009 | 19586851 |
| fungal pretreatment of lignocellulose by phanerochaete chrysosporium to produce ethanol from rice straw. | phanerochaete chrysosporium is a wood-rot fungus that is capable of degrading lignin via its lignolytic system. in this study, an environmentally friendly fungal pretreatment process that produces less inhibitory substances than conventional methods was developed using p. chrysosporium and then evaluated by various analytical methods. to maximize the production of manganese peroxidase, which is the primary lignin-degrading enzyme, culture medium was optimized using response surface methodologies ... | 2009 | 19591194 |
| optimized protocol for the 2-de of extracellular proteins from higher basidiomycetes inhabiting lignocellulose. | basidiomycetes inhabiting lignocellulose comprise an important class of filamentous fungi with ecological and biotechnological relevance. extracellular enzymes of wood-degrading fungi such as laccases, manganese-dependent (or independent) peroxidases, cellulases and xylanases are of considerable interest for biotechnological applications. still, proteomic studies of fungal secretomes based on 2-de can be very challenging due to low protein concentrations and variable amounts of fungal metabolite ... | 2009 | 19593751 |
| schema recombination of a fungal cellulase uncovers a single mutation that contributes markedly to stability. | a quantitative linear model accurately (r(2) = 0.88) describes the thermostabilities of 54 characterized members of a family of fungal cellobiohydrolase class ii (cbh ii) cellulase chimeras made by schema recombination of three fungal enzymes, demonstrating that the contributions of schema sequence blocks to stability are predominantly additive. thirty-one of 31 predicted thermostable cbh ii chimeras have thermal inactivation temperatures higher than the most thermostable parent cbh ii, from hum ... | 2009 | 19625252 |
| keratinophilic fungi on feathers of common clinically healthy birds in bahrain. | the occurrence of keratinophilic fungi associated with feather samples from 10 bird species was investigated using mycobiotic agar(®) following the incubation at 25 ± 2°c for 4 weeks. a total of 225 feather samples were cultured, of which 157 (69.77%) were found to be positive. altogether 184 fungal isolates represented by 11 species and grouped into five genera were recovered viz. chrysosporium, trichophyton, arthroderma, scopulariopsis and sepedonium. based on relative density values to rank s ... | 2011 | 19627507 |
| different drying technologies and alternation of mycobiots in the raw material of hyssopus officinalis l. | contamination of medicinal plant mass with mycobiots is one of the negative factors deteriorating the quality of raw material. in order to evaluate the impact of the yield processing technologies upon the changes of mycobiots in raw material, the mycobiotic conditions of herb hyssop (hyssopus officinalis l.) raw material were evaluated under various regimes of active ventilation and optimization of the drying parameters. the impact of ventilation intensity and temperature of drying agent upon th ... | 2009 | 19630202 |
| distribution of dermatophytes and other related fungi in jaipur city, with particular reference to soil ph. | screening of 217 soil samples of different habitats, such as pg study centre, garden, farmhouse, nursery, roadside, hostel, animal habitat, bird habitat, marriage garden, temple, vegetable market and house dust, was carried out for the presence of dermatophytes and related fungi in relation to soil ph. a total of 461 isolates belonging to 26 genera and 34 species were recorded. soil ph values vary from 3 to 10.5. trichophyton verrucosum, microsporum audouinii and m. canis were isolated for the f ... | 2011 | 19638000 |
| electrochemical evidence of self-substrate inhibition as functions regulation for cellobiose dehydrogenase from phanerochaete chrysosporium. | the reaction mechanism of cellobiose dehydrogenase (cdh) from phanerochaete chrysosporium, adsorbed on graphite electrodes, was investigated by following its catalytic reaction with cellobiose registered in both direct and mediated electron transfer modes between the enzyme and the electrode. a wall-jet flow through amperometric cell housing the cdh-modified graphite electrode was connected to a single line flow injection system. in the present study, it is proven that cellobiose, at concentrati ... | 2009 | 19640808 |
| biochemical characterization and transcriptional analysis of the epoxide hydrolase from white-rot fungus phanerochaete chrysosporium. | the white-rot basidiomycetes phanerochaete chrysosporium is a model fungus used to investigate the secondary metabolism and lignin degradation. genomic sequencing reveals the presence of at least 18 genes encoding putative epoxide hydrolases (ehs). one cdna encoding eh (designated as pcheha) was cloned and expressed in escherichia coli. transcriptional analysis demonstrated that the transcripts of pcheha could be detected under the ligninolytic and nonligninolytic conditions as well as amended w ... | 2009 | 19657565 |
| dermatomycosis in a pet inland bearded dragon (pogona vitticeps) caused by a chrysosporium species related to nannizziopsis vriesii. | a chrysosporium sp. related to nannizziopsis vriesii was isolated in pure culture from squames and biopsies of facial lesions in a pet inland bearded dragon (pogona vitticeps) in spain. the presence in histological sections of morphologically consistent fungal elements strongly incriminates this fungus as the aetiological agent of infection. lesions regressed following treatment with oral ketoconazole and topical chlorhexidine and terbinafine until the lizard was lost to follow up 1 month later. ... | 2009 | 19659541 |
| changes in the gene expression of the white rot fungus phanerochaete chrysosporium due to the addition of atropine. | we constructed a longsage (long serial analysis of gene expression) library from a 3-d culture of phanerochaete chrysosporium supplemented with atropine, which inhibits the production of lignin-degrading enzymes. the library (the atropine library) contains 13,108 longsage tags and 6,783 unique tags. the gene expression profile represented by the tags was compared with those of two previously constructed libraries, one of which was constructed using 2-d cultures in which the fungus had not yet pr ... | 2009 | 19661694 |
| the fungal glutathione s-transferase system. evidence of new classes in the wood-degrading basidiomycete phanerochaete chrysosporium. | the recent release of several basidiomycete genome sequences allows an improvement of the classification of fungal glutathione s-transferases (gsts). gsts are well-known detoxification enzymes which can catalyze the conjugation of glutathione to non-polar compounds that contain an electrophilic carbon, nitrogen, or sulfur atom. following this mechanism, they are able to metabolize drugs, pesticides, and many other xenobiotics and peroxides. a genomic and phylogenetic analysis of gst classes in v ... | 2009 | 19662500 |
| [degradation of acid blue 45 in a white-rot fungi reactor operated under non-sterile conditions]. | to operate the white-rot fungal reactor under non-sterile conditions has important significance for its practical applications. ozone (0.0144 mg/min) was selected as the bactericide to control the bacterial contamination in a white-rot fungal reactor. the control effect on bacteria, enzyme production, ph variation and decolorization of a recalcitrant dye (acid blue 45) in the reactor were studied during continuously operating the reactor. it was found that, the contamination bacteria were contro ... | 2009 | 19662871 |
| chrysosporium guarroi sp. nov. a new emerging pathogen of pet green iguanas (iguana iguana). | chrysosporium guarroi sp. nov. represented by five strains isolated from cases of dermatomycosis in pet green iguanas (iguana iguana) in spain, is described and illustrated. this taxon is characterized by its ability to grow at temperatures from 15 to 37 degrees c and by the presence of arthroconidia and aleurioconidia. the latter are unicellular, smooth, pyriform or clavate, sessile or borne at the ends of narrow stalks. the analysis of the sequences of the d1/d2 and its regions confirm the sep ... | 2010 | 19675967 |
| quantitative transcriptional analysis of the genes encoding glycoside hydrolase family 7 cellulase isozymes in the basidiomycete phanerochaete chrysosporium. | cellulolytic fungi generally secrete a cellulase mixture consisting mainly of glycoside hydrolase family 7 cellulases (cel7s) during degradation of crystalline cellulose. although several cel7s have been investigated so far, the marked similarity in their amino acid and nucleotide sequences makes independent quantitative analysis difficult. here, we present a real-time pcr method for the detection and quantification of cel7 genes (cel7a-f/g) in the basidiomycete phanerochaete chrysosporium using ... | 2009 | 19709307 |
| effects of inoculation with phanerochaete chrysosporium at various time points on enzyme activities during agricultural waste composting. | the effect of inoculation times on the enzyme activities during agricultural waste composting was determined. four runs were used: without inoculation (run a), inoculation with phanerochaete chrysosporium (p. chrysosporium) during the first fermentation phase (run b), inoculation during the second fermentation phase (run c) and inoculation during both the first and the second fermentation phase (run d). the results revealed that the effect of inoculation on carboxy methyl cellulase (cmcase) acti ... | 2010 | 19717299 |
| regulation and heterologous expression of p450 enzyme system components of the white rot fungus phanerochaete chrysosporium. | phanerochaete chrysosporium is widely used as a model organism to understand the physiology, enzymology, and genetics of lignin degradation by white rot fungi and is known for its ability to metabolize and detoxify a wide range of environmental chemicals. our pre-genomic efforts and the recent whole genome sequencing by the joint genome institute of the us-doe have revealed that this fungus carries a well developed p450 enzyme system, consisting of multiple p450 monooxygenases and a common p450 ... | 2008 | 19730708 |