| in vitro stimulation and inhibition of tumor cell growth mediated by different lymphoid cell populations. | spleen, lymph node and thymus cells from normal c57bl/6 mice or mice given injections 6 to 20 days previously with syngeneic methylocholanthrene-induced fibrosarcoma (b6mca) cells were tested for their ability to mediate growth stimulation and/or inhibition of b6mca cells in vitro. at an effector cell to tumor cell ratio of 10:1,, nucleated spleen cells from mice bearing the tumor for 6 days enhanced tumor cell growth. neither lymph node nor thymus cells from tumor-bearing mice were stimulatory. ... | 1977 | 66982 |
| reactivity of human brain antiserum with neuroblastoma cells and nonreactivity with thymocytes and lymphoblasts. | brain-associated antigens have been detected on human and mouse thymocytes. also, murine neuroblasts and brain cells have common antigens. in this study we compared the reactivity of rabbit anti-human brain (hb) serum with neoplastic neuroblasts and normal and neoplastic lymphoid cells. the binding of hb antiserum to viable cells was assessed by immunofluorescence and an indirect radiolabeled antibody assay. hb antiserum reacted with greater than 80% of neuroblasts derived from two human cell li ... | 1977 | 66986 |
| mithramycin and dipi: a pair of fluorochromes specific for gc-and at-rich dna respectively. | the at specificity of the fluorochromes dipi and dapi and the gc specificity of mithramycin are evidenced by observations in human, mouse, and bovine chromosomes. dipi and dapi produce a pattern similar to hoechst 33258 in all three species, whereas mithramycin results in a reverse pattern. the at-rich centromeric heterochromatin in mouse is brilliantly stained by dipi or dapi and remains nearly invisible after mithramycin staining. in the gc-rich centromeric heterochromatin of cattle the opposi ... | 1977 | 67077 |
| isolation and assay of rabies serogroup viruses in cer cells. | infection of cer cell cultures with field strains of rabies virus, ranging from 0 to 5 mouse brain passages, was detected by immunoflurescence within 2-4 days after infection. a fluorescent focus assay for measuring infectivity of seven rabies serogroup viruses was rapid and reproducible. rabies field strians and other rabies serogroup viruses also induced cytopathic effect, usually on initial passage. the hemadsorption-negative (had-) plaque test in bsc-1 cells was successfully applied to labor ... | 1977 | 67104 |
| genetic regulation of udp-glucuronosyltransferase induction by polycyclic aromatic compounds in mice. co-segregation with aryl hydrocarbon (benzo(alpha)pyrene) hydroxylase induction. | induction of hepatic 4-methylumbelliferone udp-glucuronosyltransferase (ec 2.4.1.17) by polycyclic aromatic compounds, such as 3-methylcholanthrene or beta-naphthoflavone, occurs in c57bl/6n, a/j, pl/j, c3heb/fej, and balb/cj but not in dba/2n, au/ssj, akr/j, or rf/j inbred strains of mice. this pattern of five responsive and five nonresponsive mouse strains parallels that of the ah locus, which controls the induction of aryl hydrocarbon (benzo[alpha]pyrene) hydroxylase (ec 1.14.14.2). induction ... | 1977 | 67114 |
| [study of the immunological specificity of the growth-inhibiting factor, a product of the mutant brachypodism-h gene in the mouse]. | | 1976 | 67132 |
| rapid chemical dehydration of biologic samples for scanning electron microscopy using 2,2-dimethoxypropane. | acidified 2,2-dimethoxypropane (dmp) has been used as a rapid chemical dehydrating agent en route to plastic embedding and ulthrathin sectioning for conventional electron miscroscopy (j histochem cytochem 23:107, 1975). we have used dmp to dehydrate biologic specimens prior to critical point drying and metal coating for scanning electron microscopy. there is no difference in either the gross architecture or the fine surface structure of mouse small intestine and trachea, rat trachea and kidney, ... | 1977 | 67137 |
| specificity of rat xenogeneic cell-mediated cytolysis for the products of the k and d loci of the mouse h-2 complex. | lewis rat lymph node (ln) cells, when cultured with irradiated mouse spleen cells, develop cytotoxic effectors that were shown to be specific for the sensitizing mouse strain. alloantisera directed to the products of the k and d loci of the target inhibited cytolysis whereas an antiserum to the i region gene products did not inhibit cytolysis even though lps-induced spleen blasts were used as targets. thus, it appears that even when effector cells from another species are generated to mouse sple ... | 1977 | 67147 |
| temperature dependence of antigen-specific rosette formation by lymphocytes from immunised mice. | spleen cell suspensions from mice undergoing a secondary response to sheep erythrocytes (srbc) contained about one tenth as many specific antigen-binding, rosette-forming cells (rfc) when they had been washed at 37 degrees c instead of 4 degrees c before rosetting. this difference was correlated with the presence of igg anti-srbc antibody in the serum, and the 37 degrees c washings of immunised spleen cells could passively allergise non-immune spleen cells at 4 degrees c for specific rosette for ... | 1977 | 67152 |
| studies on h-2 specificities on mouse tumour cells by a new microradioassay. | seven mouse tumour cell lines of different aetiology and origin were tested for the expression of surface alloantigens using twenty-four well defined h-2 alloantisera and anti-thy 1.2. we used a new radioassay that involves antibody-complement treatment of the tumour target cells followed by postlabeling the surviving tumour cells with 14c-thymidine. with a relatively high frequency the anti-h-2 sera were reacting differently with the tumour cells than with respective syngeneic lymphoid cells. t ... | 1977 | 67153 |
| molecular relationship between h-2 and ml antigens on leukaemia l-1210/v cells. | the molecular relationship between h-2 antigens and mammary leukaemia (ml) antigen at the surface of leukaemic l-1210/v cells was studied by the 'lyso-strip' method. molecules carrying the private specificities of the h-2d haplotype are independent entities in the membrane of the l-1210/v leukaemic cells and are not linked to molecules carrying the 'ml' antigen. | 1977 | 67154 |
| plasma cell antigens in human multiple myeloma. | tumor-specific antigens capable of eliciting a response from autologous lymphocytes have been described in mouse plasmacytoma systems. this paper presents evidence of similar antigens in human myeloma. myeloma plasma cells isolated from the bone marrow of 27 patients stimulated autologous and allogeneic peripheral blood lymphocytes (pbl) from myeloma patients in mixed leukocyte culture. plasma cells isolated fromt the bone marrow of normal patients or patients with benign plasmacytosis failed t ... | 1977 | 67161 |
| ia antigenic specificities are oligosaccharide in nature: hapten-inhibition studies. | we have previously reported that the ia specificities, coded for by the i region within the h-2 complex, appear to consist predominantly of carbohydrate. this conclusion was reached by examining low molecular weight ia-bearing oligosacharides isolated from mouse serum. we now report hapten-inhibition studies which indicate that the binding of both allogeneic and xenogeneic anti-ia antibodies to the ia glycoproteins found predominantly on b lymphocytes can be specifically inhibited by certain fre ... | 1977 | 67165 |
| genetic control of alpha-fetoprotein synthesis in the mouse. | to approach the genetic mechanism that turns off the synthesis of alpha-fetoprotein (afp) after birth, we assumed that a change in this mechanism might affect the low basal level of afp that can be detected in the adult organism. the concentration of afp was therefore determined for serum from adult mice of 27 different inbred strains. with one exception, this basal level was between 34 and 173 ng/ml, which is about 10(5)-fold less than the serum concentration at birth. in one strain, balb/c/j, ... | 1977 | 67170 |
| cytotoxic t lymphocytes specific for i region determinants do not require interactions with h-2k or d gene products. | gene products coded for by the major hisocompatibility complex (mhc) can serve as target antigens for cytotoxic t lymphocytes (ctl) (1). a variety of test systems are available which have yielded information consistently reinforcing the importance of this complex of genes in the generation and effector phases of the cytotoxic immune response. originally, it was shown that allogeneically-induced ctl had specificity primarily for the products of the k and d loci of the mouse h-2 complex (2). more ... | 1977 | 67179 |
| antigenic changes of dba/2j mastocytoma cells when grown in the balb/c mouse. | a 10-day growth period of the dba/2j strain-specific p815-x2 mastocytoma in the balb/c mouse altered the antigenicity of the tumor cell surface. the in vivo-modified mastocytoma cells differed from mastocytoma cells grown in the original dba/2j mice in suspectibility to lysis by immune peritoneal exudate cells, in vitro antigenic recognition by cytotoxic t-cells, and immunizing capacity in allogeneic c57bl/6 mice. propagation of the altered tumor cells in the original host or maintenance in tiss ... | 1977 | 67213 |
| yolk sac carcinoma grown from rat egg cylinders. | eight-day rat egg cylinders transplanted in toto under the kidney capsules of adult histocompatible recipients gave rise to small nodules composed of parietal yolk sac cells surrounded by reichert's membrane-like material. these yolk sac cells could grow and give rise to malignant, transplantable tumors identical to mouse yolk sac carcinomas derived from teratocarcinoma. the sera of rats with yolk sac tumors contained elevated concentrations of alpha1 fetoprotein. | 1977 | 67215 |
| rnase h and rna-directed dna polymerase: associated enzymatic activities of murine mammary tumor virus. | the rna-directed dna polymerase of murine mammary tumor virus, a type b rna tumor virus, was purified sequentially through deae-cellulose, phosphocellulose (step gradient), and phosphocellulose (linear salt gradient) chromatography followed by glycerol sedimentation centrifugation. during all stages of purification, coincident peaks of rna-directed dna polymerase activity, templated by polyribocytidylate-oligodeoxyguanidylate, and rnase h digestion of [3h]polyriboadenylate-polydeoxythymidylate w ... | 1977 | 67221 |
| some endocrine aspects of the thymus gland. | in studies of the mouse thymus, lymphocyte mitoses are seen to be most frequent in the thymus cortex. there is evidence from thymic grafts that a hypothetical factor, thymopoietin, may stimulate mitosis of thymic lymphocytes. it is a factor which is postulated to act in conjunction with the pas-positive mesenchymal reticular cells and epithelial reticular cells of the cortex. the thymus medulla is necessary for the integrity of thymic grafts, and may also elaborate a secretion for maintaining th ... | 1976 | 67231 |
| [determination of serum antinuclear factor by the immunofluorescent method using mouse liver prints]. | | 1977 | 67255 |
| carcinogenicity study of lindane in the mouse. | linadane (gamma-bhc) was investigated for its possible carcinogenic effect on 500 mice (250 males, 250 females) of the chbi: nmri (spf) strain in a study lasting 80 weeks. a substance-related production of tumours was not evident at the dosage levels employed (12.5, 25 and 50 ppm). the tumours found corresponded in type and frequency to those occurring spontaneously in this strain. electron-microscopical examination of livers, carried our at the same time, provided no evidence of lindane-induced ... | 1977 | 67663 |
| [preparation of paraffin sections for the immunofluorescent analysis of antigens of differing chemical nature]. | the author suggests a technique for preparation of paraffine sections from tissues fixed with acetone permitting to detect antigens of various chemical nature, including alpha-fetoprotein, antigens of mouse leukemia viruses, alcohol-soluble antigens of hepatocyte membranes, and some phospholipids. | 1976 | 67860 |
| [cultivation of embryonic mouse liver epithelium]. | mouse embryo liver epithelial cells maintained in vitro were connected with each other by highly permeable cell-to-cell contacts, synthesized alpha-fetoprotein and possessed the property of contact inhibition of phagocytosis. methods of cultivation of these cells are presented in this paper. | 1976 | 67864 |
| comparative studies of the cytostatic action and metabolism of 5-azacytidine and 5,6-dihydro-5-azacytidine. | 5,6-dihydro-5-azacytidine hydrochloride, a chemically stable, soluble analog of 5-azacytidine, has cytostatic activity against mouse leukemic l1210 cells grown in culture, but concentrations on the order of 10 micronm, 10-fold higher, than the parent drug, are necessary to inhibit cell growth. the addition of either cytidine or uridine protected against growth inhibition by 5-azacytidine and 5,6-dihydro-5-azacytidine, whereas thymidine potentiated the cytostatic action of both drugs. deoxycytidi ... | 1977 | 67884 |
| induction of morphological transformation in mouse c3h/10t1/2 clone 8 cells and chromosomal damage in hamster a(t1)c1-3 cells by cancer chemotherapeutic agents. | various cancer chemotherapeutic agents including alkylating agents, antimetabolites, and antibiotics or natural products were studied for their ability to produce morphological transformation in the c3h/10t1/2 clone 8 mouse cell line and chromosomal damage in the a(t1)c1-3 hamster cell line following a 24-hr exposure of each agent at different concentrations. those drugs that were known to be carcinogenic in vivo also produced morphological transformation and chromosomal damage, whereas those ag ... | 1977 | 67887 |
| impairment of primary in vitro response of new zealand mouse spleen cells to a thymic-dependent antigen. | | 1977 | 67902 |
| interaction in vitro between myocardial cells and autologous lymphocytes and sera from patients with rheumatic carditis. | the cytotoxicity of antimyocardial antibodies and peripheral blood lymphocytes from patients with rheumatic carditis on autologous myocardial cells was investigated. although sera obtained after open-heart surgery from seven out of eight patients with rheumatic carditis were bound to cultures of autologous myocardial cells, they were not cytotoxic. peripheral blood leukocytes obtained both before and after surgery from one of these eight patients aggregated around and underwent blast transformat ... | 1977 | 67921 |
| nonspecific genetic regulation of antibody responsiveness in the mouse. | four lines of mice were produced by selective breeding for quantitative agglutinin responsiveness to flagellar (f) or somatic (s) antigens (ags) of salmonellae: high (h) or low (l) responder lines to fag and h and l responder lines to sag. the salmonellae contained both f and sags, the ag used to perform the selection was the selection ag and the other was the associated ag. the selective breeding produced a progressive interline separation with an equivalent effect for both ags. after 15 genera ... | 1977 | 67954 |
| a heterophile carbohydrate moiety common to mammalian igm and erythrocytes detected by chicken igm antibody. | sequential treatment of chicken lymphocytes with normal sheep serum (nss), chicken antiserum against sheep erythrocytes (se) and formaldehyde results in rosette formation with se. the identity of reactive components and the mechanism of rosette formation has been elucidated. the binding activity of nss to chicken lymphocytes was ascribed to "natural" igm anti-species antibodies. subsequently, chicken igm anti-se antibody reacted with sheep igm bound to the lymphocyte surface, and, following form ... | 1977 | 67955 |
| cell surface changes on the mouse blastocyst at implantation. | | 1977 | 67959 |
| suppressor t cell product, which depresses the passive transfer of contact sensitivity, shares epitope(s) with the major histocompatibility complex. | specific suppressor t cell product appears in the supernatant of cultures of lymph node cells of mice injected with picryl sulphonic acid and then painted with picryl chloride. it is detected by its ability to depress the passive transfer of contact sensitivity by immune cells incubated in it. this communication shows that the product can be absorbed by antisera prepared against the major histocompatibility locus but not by antisera prepared against mouse immunoglobulin. | 1977 | 68012 |
| characterization of an rna-directed dna polymerase found in association with murine intracytoplasmic a-particles. | an rna-directed dna polymerase was found to be associated with intracytoplasmic a-particles from dba/2 mouse leukemia cells. the enzyme activity was detected after disrupting the purified particles with 2 m nacl-20 mm dithiothreitol. the presence of a divalent cation and all four deoxyribonucleoside triphosphates was essential for this enzyme activity. the enzyme had a clear preference for mg2+ over mn2+. cesium sulfate isopycnic gradient centrifugation of the dna product synthesized in the acti ... | 1977 | 68124 |
| radioimmunoassay for mammalian type c viral reverse transcriptase. | radioimmunological techniques were applied to the analysis of reverse transcriptase of mammalian type c rna viruses. the polymerase of rauscher mouse leukemia virus was purified by ion exchange and sequential affinity chromatography. radioimmunoassays that utilized the viral enzyme as a probe detected as little as 1 ng of purified polymerase. no cross-reactivity could be demonstrated between the reverse transcriptase and other known virus-coded proteins. by comparing the immunological reactivity ... | 1977 | 68126 |
| observations on a new liver inclusion in the mouse. | | 1977 | 68131 |
| rabies virus infection in mouse neuroblastoma cells. | as part of an inquiry into factors that determine the virulence of fixed rabies virus, mouse neuroblastoma cells were infected in culture with high virulence and low virulence strains of flury hep virus. low virulence virus infection differed from high virulence virus infection in (1) its more rapid production of progeny virus in the early cycles of virus infection as shown by the number of extracellular virus particles and the infectivity of the supernatant fluid; (2) its earlier development o ... | 1977 | 68172 |
| affinity chromatography of mouse alpha-fetoprotein (afp) on oestradiol-sepharose adsorbents--isolation and properties. | | 1977 | 68173 |
| antigenic relatedness of the dna polymerase of human breast cancer particles to the enzyme of the mason-pfizer monkey virus. | we have previously reported [(ohno, t., sweet, r.w., hu, r., dejak, d. & spiegelman, s. (1977) proc. natl. acad. sci. usa 74, 764-768)] on the purification and characterization of the dna polymerase from human breast cancer particles. its preference for certain synthetic templates and its ability to use a viral rna to fashion a faithful dna transcript identify it as a reverse transcriptase similar to that found in the mouse mammary tumor virus and in the mason-pfizer monkey virus (mpmv). we repo ... | 1977 | 68475 |
| a simple technique for preparing easy to read, permanent cytotoxicity tests on mouse spermatozoa. | | 1977 | 68561 |
| detection and characterization of serum ia material in the mouse. | | 1977 | 68594 |
| selection of la crosse virus variants by sentinel squirrels (sciuris carolinensis) and chipmunks (tamius striatus). | comparisons of la crosse (lac) virus strains, obtained from sentinel squirrels and chipmunks, were made using three viral markers: plaque size on vero cells, virulence in 8 days old laboratory mice, and antigenic characteristics as measured by the plaque reduction neutralization test. all strains were in their first suckling mouse brain passage. the mean plaque size of viruses isolated from squirrels was slightly larger than the mean plaque size of viruses isolated from chipmunks. there were no ... | 1977 | 68669 |
| cell-mediated immunity to mouse tumors: some recent findings. | lymphocyte mediated immune reactions play a major role in the immunological defense against antigenic tumor cells. serum factors (antigens, antigen-antibody complexes) can thwart these reactions, perhaps by interfering with a lymphocyte "activation" process. blocking factors can be eluted from lymphoid cells harvested from tumor-bearing animals. one way of increasing cell-mediated reactivity to tumor antigens appears to be to sensitize (or "activate") lymphocytes against tumor antigens in vitro. ... | 1976 | 68697 |
| [volumetric and cytologic investigations of cytolysis of ascites tumor cells provoked by triton x-100 (author's transl)]. | concentration-effectivity curves of triton x-100 activity on ascitic cells of mouse leukemia 1210 and zajdela-hepatoma in rats have been plotted by electronic determination of cell numbers, indicating only one part of cytolysis which follows a sudden decrease of cell volumes. a better characterization of the whole cytolysis can be realised by volumetricall particle counter analysis. it first displays an increase in cell volumes for several concentrations in connection with microscopically observ ... | 1977 | 68763 |
| serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. | human, piglet, mouse, foal, lamb, calf and rabbit rotaviruses all infected, but could not readily be subcultured in llc mk2 cells. cells infected with mouse and calf rotaviruses reacted by indirect immunofluorescence (fa) with convalescent serum from children, piglets, mice, foals, lambs, calves or rabbits, taken after rotavirus infection. human, calf, piglet, mouse and foal rotaviruses reacted with human, calf, mouse, foal and lamb convalescent serum by complement fixation (cf). it was not poss ... | 1977 | 68765 |
| quantitative histochemistry of sex differences of periodic acid-schiff reaction in mouse submandibular gland. | | 1977 | 68771 |
| preliminary characterization of two thymus-dependent xenoantigens from mouse lymphocytes. | preliminary characterization of two mouse thymus-dependent (t) lymphocyte xenoantigens, t25 and t200, which are selectively labelled by lactoperoxidase-catalysed iodination of t-cells, is described. both molecules are membrane-bound glycoproteins. fractionation of membrane vesicles prepared from bw5147 lymphoma cells by sedimentation through sucrose density gradients show that antigens t25 and t200 are in fractions enriched with plasma membrane. moreover antigen t200 is partially degraded when v ... | 1977 | 68776 |
| characterization of mouse mammary tumor viruses propagated in heterologous cells. | mouse mammary tumor viruses (mmtv) from three different strains of mice have been used to establish productive infections in feline and mink cell lines. the virions that are released by these cells compete completely in a radioimmunoassay for the major virion surface glycoprotein of mmtv (gp52), thus demonstrating that antigenic determinants of gp52 are viral coded. competitive molecular hybridization studies have shown that the 60 to 70 s rna's of mmtv's propagated in feline cells contain all t ... | 1977 | 68813 |
| search for oncogenic viruses in human prostate cancer. | electron microscopic, immunologic, and biochemical methods have been used in an attempt to detect and characterize oncornaviruses in human prostatic carcinoma (pca) and benign prostatic hyperplasia (bph), and in prostates of mice of high and low mammary cancer or leukemia strains. ultrastructural examination of 37 pca and nine bph specimens has revealed the presence of particles resembling type c virus in five cases of pca and one of bph, and also two different types of intracisternal virus-like ... | 1977 | 68817 |
| rna sequences specifically associated with mouse intracisternal a particles. | | 1977 | 68836 |
| education of human lymphocytes against mouse cells: specific recognition of h-2 antigens. | | 1977 | 68884 |
| variable region (fv) determinants on mouse lymphocytes. | antisera have been raised to the fv fragment derived from a mouse alpha/lambda myeloma. these had different reactivities for fv and mouse immunoglobulin and two of them appeared to detect v region framework determinants on normal mouse immunoglobulins. a large proportion of mouse lymphocytes were shown to have fv determinants on the surface which was part of the b-lymphocyte antigen receptor. most of the reacting cells were b lymphocytes. fv determinants were not detected on the majority of t ly ... | 1976 | 68917 |
| detection and molecular characterization of the thymus-brain antigen in human brain. | anti-human brain sera were found to contain antibodies reacting with determinants present on murine thymocyte plasma membranes. this determinant is borne by the thymocyte-brain antigen of mouse thymocytes. the non species-specific determinant of the thymocyte-brain system is detectable on the thymocyte-brain antigen of human and mouse brain in comparable amounts. in contrast to these findings, the allogenic thy-1 and the species-specific determinant of this system were only found on the thymocyt ... | 1977 | 68924 |
| immune response against two epitopes on the same thymus-independent polysaccharide carrier. 1. role of epitope density in carrier-dependent immunity and tolerance. | immunogenicity and tolerogenicity of two epitopes (alpha 1-6 and fitc) on the same dextran b 512 carrier were investigated. the following conclusions were made: (1) both epitopes were thymus-independent and immunogenic and tolerogenic as well. (2) the marked dose differences between the two epitopes with regard to tolerance induction were found to be a consequence of the affinity and the heterogeneity of the responding b cells as well as the epitope density employed for detecting the pfc in a pr ... | 1977 | 68926 |
| temporal expression of membrane antigens during mouse spermatogenesis. | the temporal expression of cell surface antigens during mammalian spermatogenesis has been investigated using isolated populations of mouse germ cells. spermatogenic cells at advanced stages of differentiation, including pachytene primary spermatocytes, round spermatids, and residual bodies of regaud and mature spermatozoa, contain common antigenic membrane components which are not detected before the pachytene stage of the first meiotic prophase. these surface constituents are not detected on i ... | 1977 | 68961 |
| immune suppression with supraoptimal doses of antigen in contact sensitivity. i. demonstration of suppressor cells and their sensitivity to cyclophosphamide. | immunologic suppression was induced in a mouse model of contact sensitization to dnfb by using supraoptimal doses of antigen. in these studies, in vivo measurement of ear swelling as an indication of immunologic responsiveness correlated well with measurement of in vitro antigen-induced cell proliferation. this unresponsiveness was specific, since supraoptimal doses of dnfb did not interfere with the development of contact sensitivity to another contactant, oxazolone. the decrease in responsiven ... | 1977 | 68972 |
| b-lymphocytes activation by the fc region of igg. | strong stimulation of dna synthesis (up to 150-fold) and blast transformation can be induced in mouse spleen cells by fc fragments of human igg. the mitogenic response is optimal on day 5 of culture and is dependent on the concentration of fc fragments with a sedimentation rate of 3-5s. intact igg is also stimulatory, but only when modified by heat aggregation, and produces only a 10-fold increase in [3h]thymidine uptake. the stimulation by aggregated igg is dependent on the fc portion, since ag ... | 1977 | 68990 |
| immunosuppressive factor(s) specific for l-glutamic acid50-l-tyrosine50 (gt) ii. presence of i-j determinants on the gt-suppressive factor. | the responses to the synthetic antigens, l-glutamic acid(60)-l- alanine(30)-l-tyrosine(10) (gat) and l-glutamic acid(50)-l-tyrosine(50) (gt) are controlled by genes in the i region of the mouse h-2 complex (1-3). preimmunization of the mice bearing the h-2(p,q,s) nonresponder haplotypes with gat stimulates the development of suppressor t cells that inhibit in vivo or in vitro antibody responses to gat complexed to the immunogenic carrier, methylated bovine serum albumin (gat-mbsa) (4). the copol ... | 1977 | 68991 |
| regulatory functions of hapten-reactive helper and suppressor t lymphocytes. i. detection and characterization of hapten-reactive suppressor t-cell activity in mice immunized with hapten-isologous protein conjugate. | helper and suppressor t-cell activities were detected simultaneously in the spleen cells of mice immunized with para-azobenzoate (pab)-mouse gammaglobulin (mgg). dinitrophenyl (dnp)-specific b cells were raised by immunization with dnp-keyhole limpet hemocyanin (klh) and used as the indicator b-cell population. the helper and suppressor t-cell activities were determined after adoptively transferring spleen cells from pab-mgg- primed donors and dnp-klh-primed donors into x-irradiated recipients. ... | 1977 | 68994 |
| regulatory functions of hapten-reactive helper and suppressor t lymphocytes. ii. selective inactivation of hapten-reactive suppressor t cells by hapten-nonimmunogenic copolymers of d-amino acids, and its application to the study of suppressor t-cell effect on helper t-cell development. | an experimental condition was established in vivo for selectively eliminating hapten-reactive suppressor t-cell activity generated in mice primed with a para-azobenzoate (pab)-mouse gamma globulin (mgg)-conjugate and treated with pab-nonimmunogenic copolymer of d-amino acids (d- glutamic acid and d-lysine; d-gl). the elimination of suppressor t-cell activity with pab-d-gl treatment from the mixed populations of hapten- reactive suppressor and helper t cells substantially increased apparent helpe ... | 1977 | 68995 |
| sharing of ia antigens between species. i. detection of ia specificities shared by rats and mice. | a mouse anti-rat xenogeneic antiserum, b10.d2 anti-bn, has been found to react with a subpopulation of lymphoid cells of certain mouse strains. the corresponding alloantiserum, b10.d2 anti-b10.br, reacted in analogous fashion with lymphoid cells of bn rats. in the case of the cross-reaction on mouse cells, mapping studies indicated that at least part of the reactivity was with the product of gene(s) determined by the i-a subregion of the h-2 complex. chemical isolation studies with radiolabeled ... | 1977 | 68999 |
| the specific antigen-binding cell populations of individual fetal mouse spleens: repertoire composition, size, and genetic control. | in order to analyze the genetic and physiological basis of controls affecting the generation of the repertoire of antigen-binding cells in fetal mice, we have measured the numbers of spleen cells specific for each of four antigens as a function of the total numbers of nucleated and ig-bearing cells in inbred, hybrid, and random bred fetuses. for each of the two inbred strains balb/c and cba/j, the proportion of nucleated cells specific for a given antigen was the same for all individuals of the ... | 1977 | 69000 |
| enrichment of antigen-specific b lymphocytes by the direct removal of b cells not bearing specificity for the antigen. | antigen-specific b cells (asc) were purified from other b cells by prior incubation with specific antigen followed by rosetting with erythrocytes conjugated with anti-mouse ig and sedimenting on ficoll-isopaque. this procedure allowed the removal of most of the b cells, while those speicifc for the antigen used in incubation were retained. relative to the b-cell content, asc were enriched 64- to 132-fold. the method is highly specific in that b cells primed to two different antigens, turkey gamm ... | 1977 | 69002 |
| focal chemotherapy of brain tumours using semipermeable membranes. | semipermeable silastic rubber membranes can be used to diffuse focally a variety of chemicals including antitumour drugs. of the eight drugs tested, in vitro tests showed the best diffusion with dactinomycin, mithramycin, and oncovin, and the poorest diffusion with bleomycin, fluorouracil, and thiotepa. biological testing was performed with mithramycin, dactinomycin, and oncovin using tissue cultures of human glioblastoma and subcutaneous implants of mouse ependymoblastoma. all three drugs cause ... | 1977 | 69017 |
| in search of anti-trypanosoma cruzi drugs: new leads from a mouse model. | nine of 25 carefully selected compounds (from a stock of more than 200 000 chemical species amassed principally as a result of testing against other parasitic diseases) were found to have significant suppressive activity against the parasites in the blood of a trypanosoma cruzi mouse model. eight of these compounds evaluated in this model had suppressive activity equal to or greater than the reference compound, nifurtimox. for the first time, suppressive activity against t. cruzi is reported for ... | 1977 | 69024 |
| diastereoisomeric n-tetrahydrofurfurylnoroxymorphones with opioid agonist--antagonist properties. | the two diastereoisomeric n-tetrahydrofurfurylnoroxymorphones and their hydrochlorides 1a and 1b have been prepared and studied pharmacologically; the n-(r)-tetrahydrofurfuryl derivative 1a proved to be an opioid agonist--antagonist and the n-(s)-tetrahydrofurfuryl derivative 1b a pure antagonist. as an analgesic, 1a is 25 times more potent than morphine, but it does not show morphine-like side effects in mice. in withdrawn morphine-dependent rhesus monkeys, 1a only partially suppresses abstinen ... | 1977 | 69027 |
| effect of specific immunotherapy with preimmunization against alpha-fetoprotein on a mouse transplantable hepatoma. | antibodies to autologous alpha-fetoprotein (afp) were produced in mice by immunization with rat afp. c57l/j mice with or without such antibodies were inoculated sc or ip with controlled numbers of cells of the syngeneic, afp-producing, bw 7756 hepatoma. there was a linear relationship between circulating afp and tumor mass, with elevated afp being detectable earlier than the tumor. the afp levels of the immunized mice were generally lower than those of control mice, and tumors could be detected ... | 1977 | 69032 |
| rapid grain and cell counting for cell kinetic studies. | a commercially available bacterial colony counter has been adapted for the counting of radioautographic grains over individual cells in smears and for the counting of cells in histologic sections. for radioautographic grains, the correlation coefficients between counts obtained visually by 2 observers and between counts obtained visually and with the use of the instrument were similar (r=0.999 and r=0.998, respectively). the instrument counts were obtained more rapidly than the visual counts and ... | 1977 | 69033 |
| the xy chromosome pair in mouse and human spermatocytes, visualised by silver staining. | | 1977 | 69271 |
| human gene mapping using an x/autosome translocation. | human fibroblasts containing a translocation between the x chromosome and chromosome 15 were fused with the 6-thioguanine-resistant mouse cell line, ir. resulting hybrids, selected in hat medium, retained the x/15 chromosome. hybrids which were counterselected in 6-thioguanine lost this chromosome. the x-linked markers glucose-6-phosphate dehydrogenase (g6pd), phosphoglycerate kinase (pgk), and hypoxanthine phosphoribosyl transferase (hprt), and the non-x-linked markers pyruvate kinase (pkm2) ma ... | 1976 | 69325 |
| beta2-microglobulin locus on human chromosome 15. | we have developed an autoradiographic/electrophoretic assay capable of distinguishing mouse and human beta2-microglobulin (beta2m) in spent culture media. the method is applicable to mouse and human lines and to hybrid cell lines made from them. with this technique, mouse/human hybrid cell lines were tested for the presence of human beta2m. isozyme and karyotype analyses were also carried out with the hybrids. the combined results of these studies show that the structural gene for human beta2m i ... | 1976 | 69326 |
| major histocompatibility antigens on preimplantation mouse embryos. | | 1977 | 69345 |
| rna-dependent dna polymerase activity in intracytoplasmic type-a particles of mouse mammary tumors. | intracytoplasmic type-a particles isolated from mouse mammary tumors and purified by repeated gradient centrifugations exhibit both endogenous and exogenous (template-stimulated) rna-dependent dna polymerase activities. the enzyme has a preference for magnesium ions. | 1976 | 69377 |
| retrograde axonal transport of horseradish peroxidase. uptake at mouse neuromuscular junctions following systemic injection. | eye muscles were examined histochemically for the presence of horseradish peroxidase (hrp) activity after intravenous injections of large doses of this tracer protein. hrp had penetrated through the blood vessels and diffused into the areas of motor end plates, where it outlined axon terminals at the synaptic clefts. hrp was incorporated into pinocytotic vesicles in the axons from where an intraaxonal transport in the retrograde direction to the nerve cell bodies in the brainstem followed. accum ... | 1977 | 69382 |
| biological and biochemical properties of nonidet p40-solubilized and partially purified tumor-specific antigens of the transplantation type from plasma membranes of a methylcholanthrene-induced sarcoma. | tumor-specific transplantation antigen (tsta) was solubilized from cell membranes of sarcoma meth-a with non-ionic detergent nonidet p40. soluble tsta was partially characterized by chromatographic separation and electrophoresis. the antigen responsible for tumor rejection activity had a molecular weight of approximately 70,000 daltons in the presence of detergent and an electrophoretic mobility of alpha-globulin. tsta was well separated from mouse histocompatibility antigen h-2 by a sequence of ... | 1977 | 69494 |
| dextran derivatives in single and combination chemotherapy against transplantable mouse ascites and solid tumors. | dextran, a typical homopolysaccharide without antitumor activity, was modified by palmitoylation and/or phosphorylation to yield three derivatives: palmitoyldextran phosphate, dextran phosphate, and palmitoyldextran. of these compounds, only palmitoyldextran phosphate showed growth-inhibitory activity against ehrlich solid tumor in mice. in combination therapy with mitomycin c, bleomycin, cyclophosphamide, and 5-fluorouracil, palmitoyldextran phosphate manifested strong synergistic effects again ... | 1977 | 69495 |
| cellular immunity to the mammary tumour virus in mice bearing primary mammary tumours. | | 1977 | 69543 |
| nucleolus organizers in mus musculus subspecies and in the rag mouse cell line. | silver staining has been used to detect active nucleolus organizer regions (nor's). by this criterion six mouse chromosomes, numbers 12, 15, 16, 17, 18 and 19, can have an nor. the number and distribution of chromosomes with nor's vary among inbred strains of mus musculus musculus (c57bl/6j, balb/cj, c3h/hej and c3h/stcpr1br) and in m. musculus molossinus. in a musculus x molassinus f1 hybrid, nucleolus organizers from each parent are silver stained.--chromosomes which have nor's in diploid cell ... | 1977 | 69563 |
| tolerance induction as an index of age-related changes. | tolerance to rabbit gamma globulin rgg) can be induced in newborn or three week old mice of all strains tested. in six week old sjl or nzb mice there is marked resistance against tolerance induction to rgg. this resistance increases with increasing age and is completed by the age of 12 weeks. resistance to tolerance can be observed in 3 week old sjl animals when human igg3 but not when human igg1 or rgg is employed as tolerogen. using igg3, resistance to tolerance induction can be demonstrated i ... | 1977 | 69609 |
| mouse epidermal growth factor: light and electron microscopical localisation by immunocytochemical staining. | epidermal growth factor (egf) has been localised by immuno-staining to granules of the convoluted duct cells of the submaxillary glands of mice. improved techniques of freeze drying and formaldehyde vapour fixation have resulted in a light microscopical localisation sharper than was achieved by previous methods. egf has also been identified by electron immunocytochemistry using the unlabelled antibody enzyme method. egf is present in greater quantities in male mice than in female mice but in pre ... | 1977 | 69619 |
| selective radioactive labeling of cell surface sialoglycoproteins by periodate-tritiated borohydride. | low concentrations of sodium metaperiodate induce specific oxidative cleavage of sialic acids between carbon 7 and carbon 8 or carbon 8 and carbon 9. the aldehydes formed can easily be reduced with nab3h4 to tritiated 5-acetamido-3,5-dideoxy-l-arabino-2-heptulosonic acid or 5-acetamido-3,5-dideoxy-l-arabino-2-octulosonic acid. at 0 degrees, the periodate anion penetrates the cell plasma membrane very slowly and only externally exposed sialic acids are oxidized. this was shown by (a) limited labe ... | 1977 | 69628 |
| distribution of the thy-1 antigen in cellular and subcellular fractions of adult mouse brain. | by using a cytotoxicity inhibition assay employing akr anti-c3h thymocyte antiserum, we have determined the degree of expression of the thy-1 antigen in fractions of adult mouse brain. as expressed as cytotoxicity inhibitory capacity per mg protein with c3h whole brain arbitrarily assigned a value of 1.0, the following values were found: c3h cerebral cortex, 5.8; c3h cerebral cortex synaptosomes, 2.5: c3h whole brain myelin, 0.65; c3h cerebral cortex neurons, 0.16; and c3h cerebral cortex mitoch ... | 1977 | 69659 |
| humoral immunostimulation. vii. sialic acid masks antigenic sites on an antibody-selected bariant cell line. | variant cell lines (lc1, lc2) obtained by growth of mouse l cells (l) in cytostimulatory and cytotoxic doses, respectively, of rabbit anti-l cell antiserum al) were found previously to be altered in many ways relative to the parent cell line. a major change was the reduction of those surface membrane antigens that al recognizes. these cell variants have now been found to have increased membrane sialic acid relative to l. treatment of intact variant cells with neuraminidase (50 units/ml, 37 degre ... | 1977 | 69664 |
| regulation of the immune response to tumor antigens. iii. characterization of thymic suppressor factor(s) produced by tumor-bearing hosts. | immunosuppressor t cells (ist),6 capable of inhibiting the rejection of a methylcholanthrene-induced fibrosarcoma (s1509a) in a/jax mice immune to this tumor, produced soluble factors with similar suppressive activity. the immunosuppressive factor(s) (isf) has been shown to be immunologically specific, as demonstrated by the complete loss of suppressive activity after absorption with s1509a cells, but not with cells of an unrelated syngeneic tumor. from its behavior on gel filtration, the size o ... | 1977 | 69667 |
| functional heterogeneity among the t-derived lymphocytes of the mouse. vii. conversion of t1 cells to t2 cells by antigen. | the t1 subpopulation of peripheral t cells was defined in mice by its short half life, insensitivity to anti-thymocyte sera (ats) in vivo, and slow kinetics of response to antigen. the t2 subpopulation was defined by its long life time, elimination by ats in vivo, and rapid response to antigen. mice containing only t1-type t cells were constructed by adult thymectomy (atx) followed immediately by the elimination of t2 cells by ats treatment. immunization of these mice with srbc led to the produc ... | 1977 | 69668 |
| the oxytalan fiber system in the mandibular periodontal ligament of the lathyritic mouse. | experimental lathyrism was produced in young albino mice with a diet containing 50% sweet pea seed (lathyrus odoratus). after 7 days on the lathyritic diet, sections of themandibular molar and incisor periodontal ligaments, when oxidized and treated with aldehyde fuchsin, demonstrated enhanced staining of the oxytalan fibers and numerous vessels. at this time aldehyde fuchsin or orcein also revealed marked pathological changes in the periodntal ligament of all molars. when athyrism was prolonged ... | 1977 | 69701 |
| squirrel monkey retrovirus: an endogenous virus of a new world primate. | squirrel monkey retrovirus (smrv) was isolated by cocultivation of squirrel monkey lung cells with canine cells. 3h-labeled 60-70s smrv rna, isolated from virus grown in canine cells, hybridized to the same extent and to the same cot1/2 value to the dna of all tissues of all squirrel monkeys tested; cot1/2 values show that smrv proviral sequences are present in the low repetitive range. no smrv proviral sequences were detected in tissues from a variety of other new world monkeys, old world monke ... | 1977 | 69724 |
| characterization of a retravirus isolated from squirrel monkeys. | a new retravirus (smrv) isolated from a squirrel monkey, saimiri sciureus, has an mg2+-dependen reverse transcriptase and a buoyant density of 1.17 g/cm3 in sucrose and 1.21 g/cm3 in cesium chloride, similar to the mouse mammary tumor virus and the mason-pfizer monkey virus. the polypeptide patter of smrv as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was distinct from the reported polypeptide patterns of known retraviruses. four major polypeptides of molecular weight ... | 1977 | 69728 |
| two species of type c viral core polyprotein on akr mouse leukemia cells. | two species of glycosylated type c viral core polyprotein were identified on the surface of akr spontaneous leukemia cells. one of these cell surface polyproteins was shown by immunoprecipitation to have antigenic determinants of murine leukemia virus p30, p15, p12, and p10; the other had murine leukemia virus p30, p15, and p12, but not p10, determinants. both species were also expressed on thymocytes from 6-month-old, preleukemic akr mice. | 1977 | 69729 |
| crystalloid inclusions in normal mouse macrophage. | | 1977 | 69916 |
| isolation of a type c rna virus from an established human histiocytic lymphoma cell line. | a type c rna virus has been detected in the culture fluids of the su-dhl-1 human histiocytic lymphoma cell line previously established in this laboratory. in electron micrographs, the virus closely resembled other typical mammalian type c rna tumor viruses in size and morphology. viral rna-dependent dna polymerase activity has been demonstrated in particles (densities of 1.15 and 1.22 g/ml) in the microsomal cytoplasmic fraction and in pellets of culture fluids. the enzyme is partially inhibited ... | 1977 | 70039 |
| cytotoxic effect of anti-h-2 and anti-ia antisera on human b and t cells. | anti-h-2 and anti-ia alloimmune mouse antisera were tested by the microcytotoxicity test on human peripheral blood b- and t-cell preparations. anti-h-2 antisera react by a higher titer and/or cytotoxicity scoring grade on b cels than on t cells. anti-ia antisera react practically only with b cells. it was assumed that anti-h-2 antisera contain two components. one component reacts specifically with certain hla-a- or -b-locus antigens or other closley linked gene products. the other component reac ... | 1977 | 70061 |
| [antigenic structure of the plasma membranes of mouse liver cells. iii. lipid haptens]. | the total glycolipids, gangliosides, neutral lipids and some individual lipids of these fractions were isolated from the whole lipid extract of the mouse liver cell suspension. the antigenic activity of the isolated lipids was studied with the complement fixation test using the rabbit antisera against the liver cell ghosts. active lipids were: glycosphingolipids, gangliosides, cardiolipin and fraction containing polar glyco- and phospholipid mixture. the cardiolipins isolated from animal cells o ... | 1977 | 70096 |
| [localization of alpha-fetoprotein in regenerating mouse liver. relationship to dna synthesis]. | | 1977 | 70123 |
| new formamidase having substrate specificity for o-formylaminoacetophenone in pig liver. | the supernatant (13 000 x g, 20 min) of pig liver homogenate was filtered with sephadex g-200 and formamidase (aryl-formylamine amidohydrolase, ec 3.5.1.9)activity in each fraction was measured. when formylkynurenine was used as substrate, two peaks of formamidase activity were observed but, with formylaminoacetophenone as substrate, only one peak was observed. formamidase in the lower molecular weight fraction is known as kynurenine formamidase (fa i), formamidase found here in the higher molec ... | 1977 | 70225 |
| purification and estrogen binding properties of mouse alpha1-fetoprotein and of two forms of the protein with different affinities for concanavalin-a. | | 1977 | 70232 |
| [comparative immunochemical analysis of specific beta globulins of the "pregnancy zone" of the humans and other mammals]. | by means of the immunodiffusion method it was shown that human pregnancy-specific beta-globulin, as well as analogous beta-globulins in the pregnancy serum of mammal animals (rat, guinea-pig) had an individual antigenic structure. during pregnancy the blood serum of the allied species (rat, mouse) contained immunologically similar beta-globulins which apparently were synthesized in the placenta. | 1976 | 70237 |
| [macrophage migration inhibition by t-cells in rats after overcoming natural tolerance to alpha fetoprotein]. | cellular immunity to alpha-fetoprein (afp) was studied in rats by the macrophage migration inhibition (mmi) test after breakage of tolerance to homologous afp of rat (rafp). a single injection of cross-reacting afp of mouse (mafp) has induced the capacity of peritoneal exudate cells of rats to react in the mmi test to both mafp and rafp. the second injection of mafp results in reduction of the mmi reaction to both antigens and the appearance of stimulation in the macrophage migration together w ... | 1976 | 70238 |
| [synthesis and localization of alpha fetoprotein in liver regeneration in mice]. | typical mature hepatocytes constituting not over several per cent of the total amount of preserved hepatocytes served as the principal site of the alpha-fetoprotein (afp) localization in the liver of mice regenerating after the ccl4 poisoning or partial hepatectomy. morphologically they failed to differ from the principal mass of hepatocytes and retained an antigen of the bile capillaries on the surface. a change id to the dynamics of the afp level in the animal serum. apparently in regeneration ... | 1976 | 70239 |
| [detection of antigens of endogenous viruses of the c-type during mouse development]. | embryos of the 12th-20th day of gestation, newborn and adult akr and balb/c mice were investigated for the presence of mouse c-type virus major structural p30 protein (gs-1) and gross leukemia virus type-specific antigen aglv) by means of radioimmunodiffusion with test systems. the p30 protein was distinctly determined from the 12th day of intrauterine development in both mouse lines; it persisted in the embryo tissues until birth and was detectable also in the akr and balb/c mouse tissues from ... | 1977 | 70247 |
| [characteristics of hepatocytes containing alpha-fetoprotein in regenerating mouse liver]. | induction of the synthesis of the embryo-specific protein--alpha-fetoprotein (alpha-fp) was revealed in the swr mice of different age after one or several ccl4 treatments. the alpha-fp level elevation was the greatest in mice aged under one month. morphologically in the liver regenerating after ccl4 poisoning the alpha-fp-containing hepatocytes failed to differ from the principal mass of cells, i.e. in the small cells of young animals and in the large hepatocytes of high ploidy in mice repeatedl ... | 1977 | 70250 |
| biotransformation of morphine to dihydromorphinone and normorphine in the mouse, rat, rabbit, guinea pig, cat, dog, and monkey. | biotransformation of morphine to dihydromorphinone and normorphine was studied in several mammalian species. free and total dihydromophinone, morphine, and normorphine in the urine were determined, as propionyl derivatives, with a gas-chromatographic technique. dihydromorphinone was detected as a morphine metabolite in the acid-hydrolyzed urine of all species studied except the dog and morphine-dependent man. normorphine in both free and conjugated forms was detected in the urine of all species ... | 1977 | 70327 |