| glycoprotein biosynthesis in calf kidney. glycoprotein sialyltransferase activities towards serum glycoproteins and calf tamm-horsfall glycoprotein. | cmp-acneu:glycoprotein sialyltransltransltransltransltransferase of calf kidney cortex was characterized using serum glycoproteins and tamm-horsfall glycoprotein, obtained from calf urine, as acceptors. native calf tamm-horsfall glycoprotein showed the best acceptor properties, followed by desialylated calf fetuin and desialylated human alpha 1-acid glycoprotein exhibiting v values of, respectively, 114, 63 and 41 nmol/h per g wet wt. of kidney cortex and km values of 0.12, 0.16 and 0.26 mm glyc ... | 1979 | 36173 |
| modulation of benzodiazepine binding sites in calf cortex by an endogenous factor and gabaergic ligands. | | 1979 | 36205 |
| testosterone secretion in cryptorchid and intact bulls injected with gonadotropin-releasing hormone and luteinizing hormone. | | 1979 | 36271 |
| relationship of rumen gram-negative bacteria and free endotoxin to lactic acidosis in cattle. | | 1978 | 36370 |
| latent proteinase activity of gamma-glutamyl transpeptidase light subunit. | gamma-glutamyl transpeptidase, which is composed of two unequal subunits, exhibits proteinase activity when treated with agents such as urea and sodium dodecyl sulfate. the heavy subunit is preferentially and rapidly degraded. the enzyme also degraded bovine serum albumin in the presence of urea; however, several other proteins and model proteinase substrates were not cleaved. treatment of the enzyme with 6-diazo-5-oxo-l-norleucine, a gamma-glutamyl analog, results in parallel loss of transpepti ... | 1979 | 36377 |
| positive cooperativity in guanosine 3':5'-monophosphate binding to guanosine 3':5'-monophosphate-dependent protein kinase. | | 1979 | 36381 |
| influence of hydrogen and chloride ions on the interaction between sulfaethidole and bovine serum albumin studied by microcalorimetric and acid-base titrimetric methods. | from earlier studies it is known that bovine serum albumin has one high affinity binding site and several lower affinity sites for the sulfa drug n1-(5-ethyl-1,3,4-thiadiazol-2-yl)sulfanilamide (sulfaethidole) (kostenbauder, h.b., jawad, m.j., perrin, j.h., and averhart, v. (1971) j. pharm. sci. 60, 1658-1660). this binding has been further studied using equilibrium dialysis, microcalorimetry, and ph titration technique. results of these studies show that the binding of sulfaethidole to the firs ... | 1979 | 36383 |
| regulation of bovine adrenal tyrosine 3-monooxygenase by phosphorylation-dephosphorylation reaction, catalyzed by adenosine 3':5'-monophosphate-dependent protein kinase and phosphoprotein phosphatase. | | 1979 | 36394 |
| open symposium on mastitis control in dairy herds: a positive step into the future of large scale preventive veterinary medicine in the republic of south africa. | | 1978 | 36480 |
| studies on the characteristics of a partially purified antigonadotropin from bovine pineal glands. | | 1979 | 36534 |
| marker x chromosomes and tissue-culture conditions. | | 1979 | 36560 |
| proceedings eighty-second annual meeting of the united states animal health association. | | 1978 | 36629 |
| [working values for laboratory diagnosis in cattle. 1. enzymes and metabolic products in the serum or whole blood]. | | 1979 | 36725 |
| amino acid esterification by alpha-chymotrypsin immobilized in spiropyran membrane. | | 1979 | 36851 |
| energy-dependent contraction of swollen heart mitochondria--activation by butacaine. | | 1979 | 36855 |
| a new cleavable reagent for cross-linking and reversible immobilization of proteins. | | 1979 | 36892 |
| magnetic circular dichroism studies of bovine liver catalase. | absorption, circular dichroism (cd) and magnetic circular dichroism (mcd) spectra of beef liver catalase at ph 5.0 and 6.9, and its complexes with naf, kcno, nacns, nan3 and nacn, have been measured between 250 nm and 700 nm at room temperature. the ph 6.9 native catalase mcd shows the presence of several additional transitions not resolved in the absorption spectrum. while these bands can be seen in the spectra of all the derivatives, with the exception of the cyanide, their relative intensitie ... | 1979 | 36920 |
| adrenergic stimulation of fertilizing ability in hamster spermatozoa. | | 1979 | 36933 |
| thyrotropin receptors on bovine thyroid membranes: two types with different affinities and specificities. | | 1979 | 37068 |
| methemoglobin blockade of coronary arterial soluble guanylate cyclase activation by nitroso compounds and its reversal with dithiothreitol. | | 1979 | 37124 |
| putrescine-oxidase activity in adult bovine serum and fetal bovine serum. | putrescine-oxidase activity was found in fetal bovine serum (fbs) with a ph optimum of 8.0 and in adult bovine serum (abs) with a ph optimum of 9.8. the crude fbs enzyme had a km for putrescine of 2.58 x 10(-6) m and a vmax of 0.53 nmol per hr per 50 microliter serum. aminoguanidine competitively inhibited the enzyme with a ki of 1.8 x 10(-8) m. spermidine and spermine proved competitive inhibitors of putrescine for both the fbs and the crude abs putrescine oxidases. the vmax for the abs putresc ... | 1979 | 37156 |
| oxygen binding of fetal and adult bovine hemoglobin in the presence of organic phosphates and uric acid riboside. | the p50 values of "stripped" fetal and adult bovine hemoglobin were 18.4 and 28.9 respectively. neither the oxygen-hemoglobin dissociation curve nor the hill coefficient, n, of fetal or adult bovine hemoglobin was affected by uric acid riboside (uar), 2,3-diphosphoglyceric acid (2,3-dpg), adenosine triphosphate (atp), or inositol pentaphosphate (ipp). combinations of uar and atp with adult bovine hemoglobin or 2,3-dpg and atp with fetal hemoglobin also had no effect. it was concluded that neithe ... | 1979 | 37193 |
| effect of nadp+ and its analogs on the rose bengal-sensitized photoinactivation of d-erythrulose reductase from beef liver. | upon addition of nadp+, the rose bengal-sensitized photoinactivation of d-erythrulose reductase from beef liver is prevented to a remarkable extent. adenosine 2',5'-diphosphate (2',5'-adp) also has a protective effect, but to a lesser extent. on the other hand, 2'-amp markedly enhances the photoinactivation. other nucleotides which have no 2'-phosphoryl group, such as nad+, 3'-amp, 5'-amp, adp, and nmn, are ineffective. further, only 2'-amp derivatives (nadp+, 2',5'-adp, and 2'-amp) among these ... | 1979 | 37244 |
| gamma-glutamyl transpeptidase of bovine milk membranes: distribution and characterization. | the distribution and characterization of gamma-glutamyl transpeptidase from bovine milk was examined. the enzyme was associated mainly with components of milk membrane. skim milk membrane had 41% of the total activity in whole milk whereas milk fat globule membrane showed 7%. enzyme kinetic characterization of these two membrane fractions showed they had identical amino acid specificities, ph, and sodium chloride effects, and similar apparent michaelis constants. the main enzymatic difference be ... | 1979 | 37261 |
| divergent results in radial immunodiffusion. iii. isolation and specificity test of class-specific antibodies. | a method is described for the isolation of antibodies specific for immunoglobulin class determinants from antisera containing antibodies both for the immunoglobulin class and for its subclasses, using affinity chromatography. the model system used is bovine igg with its subclasses igg1 and igg2, together with antisera raised in goats and sheep. the specificity of the isolated class-specific antibodies was demonstrated by double diffusion (di) and radial immunodiffusion (rid). the levels of total ... | 1979 | 37277 |
| adherence of mycoplasma gallisepticum to glass. | attachment of washed mycoplasma gallisepticum cells to glass was quantified with organisms in which membrane lipids were labelled with 3h. siliconization of the test tubes decreased attachment, while centrifugation increased it. attachment increased with temperature, decreased with increasing ph and ionic strength of the attachment mixture, but was unaffected by ca2+, mg2+ and edta. this suggests that ionic bonds, but not salt bridges, participate in the attachment process. glycophorin, the majo ... | 1979 | 37284 |
| an in vitro study of drug displacement interactions: warfarin-salicylate and warfarin-phenylbutazone. | the binding interactions between warfarin-salicylate and warfarin-phenylbutazone in the presence of 4 g percent bovine serum albumin at 37 degrees c were studied using equilibrium dialysis. methods of representing and analysing drug binding interactions are discussed. scatchard plots, double reciprocal plots and the like are shown to be of no use in representing drug displacement interactions since they display only one drug and they can be potentially misleading. it is argued that a preferable ... | 1979 | 37303 |
| assay of nicotinamide nucleotide transhydrogenases in mammalian, bacterial, and reconstituted systems. | | 1979 | 37401 |
| fibroblast and epidermal growth factors: their uses in vivo and in vitro in studies on cell functions and cell transplantation. | | 1979 | 37428 |
| [dna complexes with lysozyme]. | sedimentation method has been used to study hen egg-white lysozyme binding to glucosylated (from t2 phage) and non-glucosylated (from calf thymus) dna under conditions similar to physiological ones (ph 7,3--7,4, ionic strength 0.07--0.24). the results indicate that lysozyme binds cooperatively to both dna's. binding parameters have been obtained by applying the theory of one-dimensional adsorption of small molecules on a linear homopolymer. x-ray patterns of complexes with different protein cont ... | 1977 | 37432 |
| [cooperative interaction of serum albumin with quaternized poly-4-vinyl pyridine and structure of the complexes]. | interaction of bovine serum albumin (bsa) with quaternized poly-4-vinyl pyridine (pe) in aqueous solutions at ph 7 was studied. it was shown that in a wide range of the ratios of the components (nbsa/npe) soluble stable cooperative complexes were formed. at the same time a certain critical content of the protein exists at which the system loses its homogeneity. complex formation is not accompanied by protein denaturation. at smaller nbsa/npe ratios non-homogeneous distribution of protein globula ... | 1977 | 37435 |
| the role of thiamine in nervous tissue. | the possibility that thiamine (vitamin b1) has a role in nervous tissue that is independent of its well-documented coenzyme function is discussed. after reviewing the localization and metabolism of the vitamin and its phosphate esters, the effects of either thiamine deprivation or antimetabolites of thiamine on conduction and transmission, and the relationship between thiamine triphosphate and the genetic, neurological disease, subacute necrotizing encephalomyelopathy (leigh's disease), it is su ... | 1979 | 37452 |
| [comparative study of chicken liver xanthine dehydrogenase and bovine liver xanthine oxidase. dehydrogenase activity of xanthine oxidase (author's transl)]. | a method to purify bovine liver xanthine oxidase in described, with which samples of 256-fold specific activity with respect to the initial homogenate are obtained. bovine liver xanthine oxidase and chicken liver xanthine dehydrogenase with oxygen as electron acceptor exhibit similar profile in pkm and log v versus ph plots. with nad+ as electron acceptor a different profile in the pkm xanthine plot is obtained for chicken liver xanthine dehydrogenase. however three inflection points at the same ... | 1979 | 37557 |
| international committee communications. report from the subcommittee on heparin. | | 1979 | 37611 |
| influence of volume dilution, lactate, phosphate, and calcium on mitochondrial functions. | oxidative phosphorylation of isolated canine myocardial mitochondria has been evaluated after exposure to different concentrations of phosphate (5--50 mm), lactate ion in excess (5--40 mm, ph 7.4), calcium (50--270 nmol/mg protein), to lactic acidosis (ph 6.3), and to mitochondrial protein dilution (in vitro volume expansion) for 10 min to 8 h. the influence of phosphate and lactate ion addition, lactic acidosis, and in vitro volume expansion on mitochondrial function were studied in the isolati ... | 1979 | 37745 |
| analytical techniques for cell fractions. xxiv. isoelectric point stadnards for two-dimensional electrophoresis. | | 1979 | 37775 |
| carnosine binding: characterization of steric and charge requirements for ligand recognition. | | 1979 | 37805 |
| metabolic changes associated with the'downer' condition in dairy cows at abattoirs. | | 1978 | 37826 |
| effects of sulfonate anions on the self-assembly of brain tubulin. | | 1979 | 37829 |
| dicyclohexylcarbodiimide inhibits proton translocation by cytochrome c oxidase. | | 1979 | 37830 |
| cyclic amp-binding proteins : enhanced retention on membrane filters ty histone and protamine sulfate. | | 1979 | 37834 |
| studies of the kinetics of oxidation of cytochrome c by cytochrome c oxidase: comparison of spectrophotometric and polarographic assays. | | 1979 | 37897 |
| turnover and vectorial properties of cytochrome c oxidase in reconstituted vesicles. | 1. proteoliposomes containing cytochrome c oxidase and phospholipid have been made by sonication and by the cholate dialysis procedure. in both methods of preparation, only about 50% of the enzyme molecules are oriented in the membrane with their cytochrome c reaction sites exposed to the outside of the vesicle. 2. the activity of cytochrome c oxidase in the reconstituted vesicles in not increased by incubation in 1% tween 80. experiments on reconstituted vesicles containing internal (entrapped) ... | 1979 | 37902 |
| identification, characterization and localization of a (ca2+ + mg2+)-activated purine nucleoside triphosphate phosphohydrolase from calcifying cartilage. | a purine nucleoside triphosphate phosphohydrolase (unspecified diphosphate phosphohydrolase, ec 3.6.1.15) was chromatographically separated from the bulk of alkaline phosphatase activity by gel filtration chromatography of butanol and edta extracts of fracture callus and bovine epiphyseal cartilage. the callus enzyme differed from alkaline phosphatase in a variety of characteristics. the purine nucleoside triphosphate phosphatase hydrolyzed a more specific group of substrates, required ca2+ and ... | 1979 | 37914 |
| the reaction of cow beta-lactoglobulin with tetracyanoaurate(iii). | the reaction of cow beta-lactoglobulin with au(cn)-4 is shown to label the free sulphydryl group on the protein by a reductive mechanism yielding a stable complex. high salt concentrations are shown to affect the ph-dependent conformational transition (n in equilibrium r yields s). it is hoped that au(cn)-4 may be of more widespread use as a specific heavy-atom label for the isomorphous replacement method. | 1979 | 37917 |
| microhardness indentations on artificial white spot lesions. | | 1979 | 38008 |
| species differences of the stereoselective binding of phenprocoumon and warfarin enantiomers to serum albumins. | | 1979 | 38035 |
| conformational changes in bovine-liver glutamate dehydrogenase: a spin-label study. | a spin-labelled analogue of p-chloromercuribenzoate reacts specifically with glutamate dehydrogenase. the most marked change in the properties of the spin-labelled enzyme is a fivefold decrease in the rate of reduction of the coenzyme by l-glutamate and no change in the rate of oxidation by 2-oxoglutarate. the electron spin resonance spectrum is a sensitive probe for the conformational state of the enzyme. spin-labelled glutamate dehydrogenase in the presence of saturating concentrations of nadp ... | 1979 | 38111 |
| magnetic-resonance studies of the geometry of bound substrate, coenzyme and activator on bovine-liver glutamate dehydrogenase. | adp and atp with a spin-label linked to the terminal phosphate are activators of glutamate dehydrogenase and bind to the same site as the activator adp. there is hardly any interaction with the coenzyme site. glutamate dehydrogenase can be modified with a ketone spin-label at a site in the active centre[andree and zantema, (1978) biochemistry, 17, 778--783]. the spin-labelled activators interact with ketone spin-labelled glutamate dehydrogenase in the same way as with native glutamate dehydrogen ... | 1979 | 38112 |
| interaction of adp with skeletal and cardiac myosin and their active fragments observed by proton release. | the technique of proton release measurement has been used to explore the binding of adp to skeletal and cardiac myosins and their active fragments in a variety of conditions. it has proved possible to obtain binding profiles on intact myosin in the filamentous, undissolved form in physiological solvent conditions. binding constants are given. at higher ionic strength (0.5 m potassium chloride) the binding profile of magnesium-adp. is compatible with the presence of two types of site, differing f ... | 1979 | 38115 |
| protein methylase from calf liver nuclei: enzyme characterization and stimulation by serum albumins. | a protein methylase from calf-lifer nuclei was partially purified by sonication of the nuclear pellet at high ionic strength, chromatin removal and ammonium sulphate fractionation of the solubilized activity. | 1979 | 38134 |
| kinetics of acetyl-coa synthetase-ii. products inhibition studies. | | 1979 | 38154 |
| properties of extracellular neuraminidase produced by group a streptococcus. | extracellular neuraminidase production by group a streptococci was examined in 92 strains. fourteen of these strains produced appreciable amounts of enzyme; 12 of the neuraminidase-producing strains belonged to t types 1, 4, and 12. production of the enzyme paralleled bacterial growth in culture and was maximal in medium containing 0.2% glucose. the enzyme produced by one of these strains was partially purified by ammonium sulfate fractionation and filtration on g-200 sephadex. its molecular wei ... | 1979 | 38208 |
| solar heating of mammals: observations of hair transmittance. | | 1978 | 38210 |
| alpha hydroxylation of lignoceric acid in brain. subcellular localization of alpha hydroxylation and the requirement for heat-stable and heat-labile factors and sphingosine. | | 1979 | 38244 |
| nonpeptidyl amine inhibitors are substrates of lysyl oxidase. | | 1979 | 38246 |
| electron spin resonance study of the role of no . catalase in the activation of guanylate cyclase by nan3 and nh2oh. modulation of enzyme responses by heme proteins and their nitrosyl derivatives. | the role of no . catalase in the activation of partially purified soluble guanylate cyclase of rat liver by nan3 and nh2oh was examined by electron spin resonance (esr) spectroscopy. equilibration of bovine liver catalase with no resulted in formation of a paramagnetic species exhibiting a three-line esr spectrum similar to that of no . catalase. this paramagnetic complex produced concentration-dependent stimulation of preparations of partially purified guanylate cyclase that were devoid of dete ... | 1979 | 38248 |
| energy-linked nicotinamide nucleotide transhydrogenase. properties of proton-translocating and atp-driven transhydrogenase reconstituted from synthetic phospholipids and purified transhydrogenase from beef heart mitochondria. | | 1979 | 38254 |
| proceedings of the idf symposium on the physics and chemistry of milk proteins. ayr, scotland, 9-11 may 1978. | | 1979 | 38262 |
| the association of bovine sh-kappa-casein at ph 7.0. | the self-association of sh-kappa-casein at ph 7.0 in 0.01 m-edta, 0.001 m-dithiothreitol buffer, containing 0.1, 0.2, 0.5 or 1.0 m-nacl is of a monomer-polymer type. the polymer is a spherical particle, diam. 23 nm and mol. wt 570000 (30 monomers). these parameters are not greatly influenced by variations in ionic strength above 0.1. the critical micelle concentration, which is a property of a monomer--polymer equilibrium, decreases with increasing ionic strength. the standard free energy of ass ... | 1979 | 38263 |
| the equilibria of calcium and phosphate ions with the micellar calcium phosphate in cow's milk. | calorimetric measurements of the heat of solution of micellar ca phosphate suggest that this material more closely resembles oh-apatite than amorphous ca phosphate or any other crystalline form. preliminary computer calculations of the ionic equilibria of milk salt solutions indicate that, over a wide range of ph and temperature, such solutions are supersaturated with respect to oh-apatite and to various other ca salts under certain conditions. | 1979 | 38264 |
| covalent bonds formed in proteins during milk sterilization: studies on caseins and casein peptides. | formation of covalent bonds at milk sterilization temperatures was studied using caseins and casein peptides. at 120 degrees c lysinoalanyl residues produced even at ph 7.0 were derived from intra-molecular interactions between phosphoserine and lysine; the conditions of formation were determined. it was also found that the formation fo isopeptidic cross-links was significant with conditions more severe than those used for milk sterilization. | 1979 | 38265 |
| titrimetric studies on milk and milk products. | potentiometric acid-base titration is a simple method that can be used successfully in the investigation of the protein system of milk and other protein-containing products. its applications include the examination of conventional heating procedures, cheese ripening, the investigation of processed cheese and the effect of melting salts. the principle depends on the performance of ph-dependent phase transitions which are recorded by a single titration cycle and evaluated quantitatively by means o ... | 1979 | 38266 |
| ribonuclease activities in various classes of nuclei from bovine cerebrum. | | 1979 | 38302 |
| lipid metabolism in the mammary gland of ruminant animals. | | 1979 | 38463 |
| apamin, a centrally acting neurotoxic peptide: binding and actions. | | 1979 | 38653 |
| hepatic lipid metabolism: effect of spermine, albumin, and z protein on microsomal lipid formation. | | 1979 | 38748 |
| the ca2+-induced membrane transition in mitochondria. ii. nature of the ca2+ trigger site. | | 1979 | 38751 |
| an improved fluorimetric determination of alpha-mannosidase activity in bovine plasma. | this paper describes a manual fluorimetric method for the assay of acidic alpha-mannosidase activity in bovine plasma. the optimum conditions for the assay of this enzyme were studied. the assay method devised includes the addition of zinc to the substrate, which stimulates activity by approximately twofold, and reduces the optimum substrate concentration. this latter feature affords considerable cost saving in each test. we have also shown that the alpha-mannosidase activity in lithium heparin ... | 1979 | 38770 |
| investigation of the interactions of oxytocin with neurophysins at low ph using carbon-13 nuclear magnetic resonance and carbon-13-labeled hormones. | the specifically 13c-labeled (90% 13c-enriched) peptide hormone derivatives [1-hem[2-13c]cystine]oxytocin, [1-hemi[1-13c]cystine]oxytocin, and [2-[-2-13c]tyrosine[-oxytocin and the analogue [3-[2-13c]leucine]oxytocin were prepared by total synthesis and used to study the interactions of the neurohypophyseal hormones with the bovine neurophysins as a function of ph and temperature. under all conditions, whether high or low ph, the chemical shifts of the labeled carbon atoms of the bound hormones ... | 1979 | 38833 |
| comparative studies of tyrosine modification in pancreatic phospholipases. 2. properties of the nitrotyrosyl, aminotyrosyl, and dansylaminotyrosyl derivatives of pig, horse, and ox phospholipases a2 and their zymogens. | | 1979 | 38834 |
| affinity labeling of histidine and lysine residue in the adenosine deaminase substrate binding site. | 1. adenosine deaminase was inactivated by 9-(4-bromoacetamidobenzyl)-adenine (i) and 9-(2-bromoacetamidobenzyl)adenine (ii), two affinity labels. 2. the stoichiometry of the reaction with reagent ii is reported: 1 mol reagent is bound per mol inactive enzyme. amino acid analysis of the 6 n hcl hydrolyzate of the inactive enzyme identified cm-histidine as the main alkylation product. this is the first evidence of the presence of a histidine in the active site region. 3. the alkylation rate and in ... | 1979 | 38849 |
| dna polymerase activity of lymphocyte nuclei of cattle lymph in the normal state and in lympholeukemia. | a comparative study wad made of the dna polymerase activity of lymphocyte nuclei of cattle lymph in the normal state and in lympholeukemia. no significant differences were found in the specific dna polymerase activity of the nuclei of normal and leukemic lymphocytes, both in the absence and in the presence of exogenous "activated" dnas. the dna polymerase activity of both types of nuclei was a maximum at ph 7.8 and a mg2+ concentration of 4--6 mm. the dna polymerase activities of lymphocyte nucl ... | 1978 | 38860 |
| certain aspects of hemorheology in a near zero gravity environment. | | 1979 | 38870 |
| peptides obtained from elastin by hydrolysis with aqueous ethanolic potassium hydroxide. | | 1979 | 38948 |
| neuroleptic receptors: stereoselectivity for neuroleptic enantiomers. | in order to identify a pair of neuroleptic enantiomers with the highest stereoselective interaction with neuroleptic/dopamine receptors, the effects of eight pairs of neuroleptic enantiomers were tested on the specific binding of 3h-spiperone to crude homogenates of calf caudate nucleus. the ratios of the ki values were: (+)-butaclamol/(-)-butaclamol = 3000; dexclamol/(-)-analogue = 151; (+)-isobutaclamol/(-)-isobutaclamol = 146; (-)-ctc/(+)-ctc= 109; (-)-centbutindole/(+)-centbutindole = 20; s( ... | 1979 | 38971 |
| hydrodynamic properties of bovine cardiac troponin. | | 1979 | 39000 |
| immunocytochemical localization of neuronal antigens: tyrosine hydroxylase, substance p, [met5]-enkephalin. | neuronal antigens can be demonstrated histologically by numerous direct and indirect immunocytochemical techniques in which a specific antibody is identified by a marker compound such as fluorescein isothiocyanate, ferritin, or horseradish peroxidase. one of the more sensitive methods for the light and electron microscopic localizations of antigens in sections of tissue is the peroxidase-antiperoxidase (pap) technique. the experimental procedures and the results obtained using this technique for ... | 1979 | 39006 |
| glucose-6-phosphate dehydrogenase from bovine erythrocytes: kinetic properties and the electrophoretic comparison of its subunit structure with g6pdh from other sources. | | 1979 | 39009 |
| immunization of calves against enterotoxigenic colibacillosis by vaccinating dams with purified k99 antigen and whole cell bacterins. | pregnant cattle were either vaccinated subcutaneously with (i) a suspension of purified escherichia coli k99 pili, (ii) a formalin-killed whole cell bacterin containing enterotoxigenic e. coli strain b44 (o9:k30;k99:h-), or (iii) a bacterin containing six different strains of bovine enterotoxigenic e. coli (multiple-strain bacterin), or were left as nonvaccinated controls. after birth, calves were allowed to nurse their dams and, at 12 to 14 h of age, were challenged orally with 10(11) cells of ... | 1979 | 39031 |
| adherence of erythrocytes to mycoplasma pneumoniae. | the human pathogen mycoplasma pneumoniae adheres to a variety of cells, including erythrocytes. a hemadsorption technique was developed to quantitate adherence by photometric measurement of lysates of erythrocytes that attached to sheets of m. pneumoniae grown in cups of linbro plates. attachment of sheep erythrocytes (se) increased with higher ionic strength, was unaffected by minor ph variations (6 to 9), and was blocked by anti-m. pneumoniae antiserum, but was not inhibited by a variety of su ... | 1979 | 39034 |
| isolation of yersinia enterocolitica-resembling organisms and alteromonas putrefaciens from vacuum-packed chilled beef cuts. | | 1979 | 39056 |
| differences between the reactivities of two pyridine nucleotides in the rapid reduction process and the reoxidation process of adrenodoxin reductase. | the reaction process of adrenodoxin reductase with nadph and nadh were investigated. the appearance of new intermediate with a broad absorption band at around 520 nm has been detected by rapid-scan stopped-flow spectrophotometry. although the formation of this intermediate is more rapid with nadph than with nadh, the rates of the subsequent decay to the fully reduced state are almost identical (kobs values were 20.5 and 16.0s-1). these results indicate that the new intermediate is the complex fo ... | 1979 | 39065 |
| relaxation of bovine coronary artery and activation of coronary arterial guanylate cyclase by nitric oxide, nitroprusside and a carcinogenic nitrosoamine. | the principal objective of this study was to test the hypothesis that nitroprusside relaxes vascular smooth muscle via the reactive intermediate, nitric oxide (no), and that the biologic action of no is associated with the activation of guanylate cyclase. nitroprusside, n-methyl-n'-nitro-n-nitrosoguanidine (mnng) and no elicit concentration-dependent relaxation of precontraced helical strips of bovine coronary artery. nitroprusside, mnng and no also markedly activate soluble guanylate cyclase fr ... | 1979 | 39089 |
| program seventy-fourth annual meeting of the american dairy science association, utah state university, logan, utah june 24-27, 1979 [abstracts]. | | 1979 | 39090 |
| investigation of the system cobalt(ii) bovine carbonic anhydrase b-trichloroacetaldehyde. | the interactions between hydrated trichloroacetaldehyde and cobalt(ii)bovine carbonic anhydrase b have been investigated as a function of ph by means of electronic spectroscopy of ft nmr spectroscopy. the hydrated aldehyde is bound to the metal ion and its apparent affinity constant is ph dependent with a bell-shaped profile. the kinetic parameters of the dissociation process have also been determined. | 1979 | 39117 |
| in vitro binding of trypanosoma congolense to erythrocytes. | trypanosoma congolense broden, an intravascular parasite, binds to vessel walls and erythrocytes of infected hosts. in an attempt to characterize t. congolense adhesion to host cells, an in vitro assay was devised. it was shown in the in vitro experiments that t. congolense binds to bovine, sheep, and goat erythrocytes, but not always to erythrocytes of rats, mice, rabbits, horses or humans. only the anterior part of live trypanosomes adheres to erythrocytes, and the attachment site on the trypa ... | 1979 | 39167 |
| effect of purified phospholipases on the binding of tetrodotoxin to axon plasma membrane. | the role of phospholipids in the binding of [3h]tetrodotoxin to garfish olfactory nerve axon plasma membrane was studied by the use of purified phospholipases. treatment of the membranes with low concentrations of either phospholipase a2 (crotalus adamanteus and naja naja) or phospholipase c (bacillus cereus and clostridium perfringens) resulted in a marked reduction in tetrodotoxin binding activity. a 90% reduction in the activity occurred with about 45% hydrolysis of membrane phospholipids by ... | 1979 | 39172 |
| psychoactive butyrophenones: binding to human and bovine serum albumin. | | 1979 | 39299 |
| [studies on the persistence of clostridium botulinum on a cattle farm (author's transl)]. | in the winter of 1978-1979, the presence of clostridium botulinum was studied on a cattle farm, on which botulism caused by feeding the animals contaminated brewers' grains occurred in 1977. cl. botulinum type b, the cause of mortality among cattle at the time, was detected in grass silage prepared in 1978. this organism was not detectable in a grass silage pit dating from 1977 and made prior to the outbreak of botulism. investigations showed that proteolytic types of c. botulinum having grass a ... | 1979 | 39368 |
| [urocaninase in the blood, serum and liver of cows]. | | 1979 | 39378 |
| [comparative tests of various liquid media for the preenrichment of salmonellae from milk powder (author's transl)]. | in applying s. mendoza the following four liquid media were tested as to whether they can be used as enrichment broths for salmonellae in milk powder: phosphate buffered water, correspondingly buffered peptone broth, tetrathionate and selenite lactose broth. the yield of salmonellae in these tests is largely independent of the starting ph value of the prepared milk suspension which was varied between 5.8 and 8.2 (fig. 1). in water the ph dropped within 24 hours (fig. 2), in peptone and tetrathio ... | 1979 | 39412 |
| preparative flatbed electrofocusing in granulated gels with natural ph gradients generated from simple buffers. | | 1979 | 39470 |
| [interferon inducing activity of rabies cell culture vaccine in humans]. | rabies cell culture vaccines are able to induce circulating interferon in human sera. in 8/15 cases a low peak of interferon appears in the serum about 8 h after the vaccination. the inhibition has been considered as due to interferon because of the resistance to ph 2 and lack of activity on other animal species. | 1979 | 39484 |
| survival of coxsackievirus b3 under diverse environmental conditions. | the survival of coxsackievirus b3 was studied under various conditions of incubation. the comparative study demonstrated that coxsackievirus b3 was stable for 24h (less than 0.4-log decrease in titer) when suspended at neutral ph (6 or 23 degrees c) in the presence of 0.25% bovine serum albumin in saline regardless of whether the preparations were subjected to evaporation. bovine serum albumin provided increased stability to the virus for each of the conditions tested. at 37 degrees c, evaporati ... | 1979 | 39495 |
| cow brain glutaminase: partial purification and mechanism of action. | | 1979 | 39511 |
| nadh- and nadph-dependent formation of superoxide anions by bovine heart submitochondrial particles and nadh-ubiquinone reductase preparation. | 1. both nadh and nadph supported the oxidation of adrenaline to adrenochrome in bovine heart submitochondrial particles. the reaction was completely inhibited in the presence of superoxide dismutase, suggesting that superoxide anions (o(2) (-)) are responsible for the oxidation. the optimal ph of the reaction with nadph was at ph7.5, whereas that with nadh was at ph9.0. the reaction was inhibited by treatment of the preparation with p-hydroxymercuribenzoate and stimulated by treatment with roten ... | 1979 | 39543 |
| (diisopropylphosphoryl)serine proteinases. proton and phosphorus-31 nuclear magnetic resonance-ph titration studies. | | 1979 | 39591 |
| purification and characterization of a long-chain acyl-coa hydrolase from rat liver microsomes. | a long-chain acyl-coa hydrolase from rat liver microsomes has been purified by solvent extraction and gel chromatography to homogeneity as judged by polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate. the enzyme was a monomer of molecular weight 59 000. in a sucrose gradient it sedimented at 4.3 s. the isoelectric point, pi was 6.9, and the stokes radius was approx. 31 a. the enzyme hydrolyzed long-chain fatty acyl-coa (c7--c18) with maximum activity for pal ... | 1979 | 39618 |
| astroglial and axonal proteins in isolated brain filaments. i. isolation of the glial fibrillary acidic protein and of an immunologically active cyanogen bromide peptide from brain filament preparations of bovine white matter. | the glial fibrillary acidic protein and an immunologically active cyanogen bromide peptide were purified by immunoaffinity chromatography from 8 m urea extracts of brain filament preparations isolated from bovine white matter according to norton's procedure. the protein accounted for approximately 30% of the total protein in this preparation and for the largest fraction in the 50 000 molecular weight range. the fraction not absorbed to the immuno-sepharose column reacted with neurofilament antis ... | 1979 | 39623 |