| antibacterial activity of antisera against homologous and heterologous escherichia coli of porcine origin. | fourteen enteropathogenic and five nonenterotoxigenic escherichia coli strains isolated from pigs were used for producing antisera in rabbits and pigs. these antisera were used in an vitro test system for antibacterial activity against homologous and heterologous porcine e. coli strains. antibacterial titres were determined against the homologous strains and the percent reduction in cfu/ml caused by a 1/200 dilution of the sera against heterologous strains was determined. the results indicated t ... | 1975 | 46176 |
| antigen recognition in delayed-type hypersensitivity. | | 1975 | 46185 |
| the effect of postmortem trauma on neuronal cell types strained histochemically for phospholipids. | | 1975 | 46202 |
| [changes in the antigenic structures of tissue proteins under the effects of organomercury compounds]. | | 1975 | 46210 |
| an attempt to identify the intestinal receptor for the k88 adhesin by means of a haemagglutination inhibition test using glycoproteins and fractions from sow colostrum. | the k88 antigen of escherichia coli specifically adheres to the piglet intestinal cell; a solution of this antigen agglutinates guinea-pig red cells at 4 degrees c. the latter reaction was used as a model of the former, using inhibition of haemagglutination as an index of specific combination with the k88 adhesin. inhibition was found with mucous glycoproteins and chemical modification of their heterosaccharide residues by mild acid hydrolysis, periodate oxidation or the smith degradation proced ... | 1975 | 46271 |
| letter: assay of thymosin in blood. | | 1975 | 46377 |
| minor calyces as primary pacemaker sites for ureteral activity in man. | in isolated preparations of human upper-urinary-tract muscle in the organ-bath, calyceal and subcalyceal areas behave differently. minor calyx preparations invariably exhibit immediate and extremely regular rhythmical contractions, in direct contrast to preparations of major calyx/pelvis which remain completely quiescent. specimens of ureter do contract spontaneously but only after periods up to 1 hour. smooth muscle of the minor calyces possesses excitatory alpha-adrenoceptors, as does that of ... | 1975 | 46391 |
| editorial: rotaviruses of man and animals. | | 1975 | 46393 |
| letter: populations of guineapig lymphocytes displayed by nomarski microscopy. | | 1975 | 46537 |
| letter: prostaglandins and asthma. | | 1975 | 46549 |
| infection, anergy and cell-mediated immunity. | | 1975 | 46586 |
| ir-gene control of immunogenicity of insulin and a-chain loop as a carrier determinant. | | 1975 | 46593 |
| alcian blue-tartrazine; a simple differential stain for the fundic gland cells of mammalian stomach. | | 1975 | 46639 |
| formation of cysteinyldopa from glutathionedopa in melanoma. | glutathionedopa injected intravenously into mice is metabolized and excreted in the urine as a compound with the fluorescence characteristics of cysteinyldopa. glutathionedopa incubated with a guinea-pig kidney homogenate is metabolized to a compound with the fluorescence characteristics of cysteinyldopa. boiling of the kidney homogenate prevents the metabolism of glutathionedopa. incubation of glutathionedopa with a homogenate of a melanoma metastasis led to the formation of a compound with the ... | 1975 | 46665 |
| genetic aspects of diseases of complement: an explosion. | | 1975 | 46701 |
| effects of human chorionic gonadotropin preparations on complement in vitro. | the effects on complement in vitro by several commercial preparation of human chorionic gonadotropin (hcg) were investigated. these preparations were demonstrably anticomplementary and produced marked conversion of the third component of complement (c'3). however, detailed analyses showed that the anticomplementary activity was because of the presence of immunoglobulins in the hcg preparations. in view of the known modifying effects of c'3-reactive agents on skin allograft survival and of antige ... | 1975 | 46710 |
| inhibition by serum of encephalitogenic activity of myelin basic protein: nature of the serum factor responsible. | | 1975 | 46785 |
| the interaction of antibodies to two myelin proteins. | antibody has been prepared to a hydrophobic myelin protein in rabbits using several different adjuvants. this antibody was found to react with the myelin basic protein. this latter activity could be absorbed out with basic protein leaving the reactivity to the hydrophobic protein, suggesting that the two myelin proteins shared a common antigenic determinant. in addition, the hydrophobic protein appears to possess a unique antigenic determinant. | 1975 | 46838 |
| localization of a protein antigen in the chicken spleen. effect of various manipulative procedures on the morphogenesis of the germinal centre. | the time course of the localization of a protein antigen human serum albumin (hsa) into the chicken spleen after intravenous injection is analysed. localization within seconds to the region surrounding the schweigger-seidel sheaths is accomplished by hsa complexes with chicken anti-hsa or by heat aggregated hsa. the localization of soluble hsa has to await the synthesis of sufficient chicken anti-hsa to accomplish localization to the same white pulp sites in the spleen at 25-30 hours after injec ... | 1975 | 46839 |
| chemistry of allergens. xxii. isolation and characterization of three new antigens from the dialysates of six successive pepsin hydrolyses of beta-lactoglobulin. | twelve new antigens previously have been demonstrated in the pepsin digests of milk proteins. the term "new antigen" is defined as an antigen with a specificity distinct from that of the protein from which it was generated. three new antigens (alpha-, beta-, and d2i) polypeptides and one nonantigenic gamma-polypeptide have been isolated from the dialysates of six successive pepsin hydrolyses of beta-lactoglobulin. the alpha- and beta-polypeptides were 1/5 and d2i was 1/10-1/20 as potent immunoge ... | 1975 | 46850 |
| determination of serum c9 level by immunodiffusion. elevation in patients with infectious or allergic skin diseases. | the measurement of the ninth component of complement (c9) in human serum can be easily carried out by the mancini method using antiserum raised against purified c9. the average c9 level in 29 healthy adults was 44.5 plus or minus 10.6 mug/ml. the serum c9 level was often elevated in patients with infectious or allergic skin diseases. the c9 level remained normal in most patients with collagen diseases. the availability of the monospecific antiserum has permitted identifying c9 in human serum as ... | 1975 | 46851 |
| the immune response against myelin basic protein in two strains of rat with different genetic capacity to develop experimental allergic encephalomyelitis. | after challenge with guiena pig basic protein (gpbp) lewis (le) rats, which are homozygous for the immune response experimental allergic encephalomyelitis (ir-eae) gene, developed positive delayed skin tests against gpbp and the 43 residue encephalitogenic fragment (ef); in addition, le rat lymph node cells (lnc) were stimulated and produced migration inhibitory factor (mif) when incubated in vitro with these antigens. in contrast brown norway (bn) rats, which lack the ir-eae gene, did not devel ... | 1975 | 46914 |
| evidence for specific suppression in the maintenance of immunologic tolerance. | specific suppressor cells have been demonstrated in mice tolerant to the thymus-dependent antigen hgg. transfer of normal thymocytes, normal spleen cells, or immune spleen cells into these tolerant mice did not restore immunocompetence to hgg. furthermore, the transfer of tolerant spleen cells into normal recipients abrogated the response of these recipients to subsequent challenge with immunogenic hgg. spleen cells removed from mice 5, 8, or 11 wk after the induction of tolerance specifically s ... | 1975 | 46917 |
| letter: malignant hyperthermia: a human and procine stress syndrome? | | 1975 | 46983 |
| letter: asymptomatic non-fatal canine rabies. | | 1975 | 47030 |
| letter: glucagon in pathogenesis of diabetes. | | 1975 | 47126 |
| chemistry of heparin. a short review on recent chemical trends. | | 1975 | 47192 |
| antimicrobial drugs in food-producing animals. control mechanisms of governmental agencies. | | 1975 | 47207 |
| basic fuchsin picric acid method to detect acute myocardial ischemia. an experimental study in swine. | application of the hematoxylin basic fuchsin-picric acid method to human postmortem myocardium specimens failed to show reproducible results. therefore, controlled animal studies utilizing the method were undertaken. myocardial ischemia was induced in swine by ligation of the anterior descending coronary artery for periods of 30 minutes to six hours. electron microscopy was used to monitor normal and ischemic myocardium. reproducible results were not obtained by staining successive slides of ... | 1975 | 47242 |
| beta-adrenoceptors invloved in inhibition of histamine release from sensitized guinea-pig lung. | | 1975 | 47291 |
| carrier and hapten functions in immune deviation. i. contrary effects of hapten and carrier on cellular and helper functions of t cells. | carrier and hapten functions have been studied in the immune deviation phenomenon. delayed hypersensitivity to the carrier and anaphylaxis and arthus hypersensitivities to the hapten and to the carrier were studied in guinea-pigs injected intravenously with large doses of carrier, homologous and heterologous hapten-carrier conjugates and subsequently immunized with the hapten-carrier conjugate in freund's complete adjuvant. pretreatment with dnp-bsa or with hgg were found to modify, in opposite ... | 1975 | 47307 |
| genetic control of sensitization to structurally unrelated antigens and its relationship to histocompatibility antigens in guinea-pigs. | five strains of guinea-pigs homozygous for gpl-a and 2/13 histocompatibility antigens were sequentially immunized with seven structurally unrelated antigens (tgal, bpo-bgg, asan, bpo-pll, phenetidine, horseradish peroxidase and dnp-dodecapeptide). considerable differences in the immune response were observed, as measured by delayed skin reactivity and antigen-induced lymphocyte stimulation in vitro. the data presented show that when the progeny of various back-cross matings are analysed for thei ... | 1975 | 47308 |
| studies of the inhibition of c56-initiated lysis (reactive lysis). iv. antagonism of the inhibitory activity c567-inh by poly-l-lysine. | the stable intermediate complex c56 can initiate the lysis (reactive lysis) of unsensitized erythrocytes (e) by the membrane attack machanism of complement. certain serum constituents designated c567-inh inhibit reactive lysis by preventing the c567 complex, once formed, from attaching to a membrane surface. it is shown here that microgram quantities of poly-l-lysine (pll), a synthetic polycation of molecular weight 180,000, can reverse the effests of c567-inh, and thereby potentiate formation o ... | 1975 | 47309 |
| role of the chondrocytes in the breakdown of pig articular cartilage induced by complement-sufficient antiserum to pig erythrocytes. | previous work showed that in organ culture pig articular cartilage is not affected by complement-sufficient antiserum to pig erythrocytes (as plus c') unless it is associated with soft connective tissue. in the presence of synovial tissue the matrix becomes depleted of proteoglycan and may finally disintegrate. immunoglobulin fails to enter normal cartilage matrix, but penetrates depleted matrix and reacts with the surfaces of the chondrocytes. the present experiments were made to see whether th ... | 1975 | 47316 |
| compound d+y+ particles of australia antigen. | australia antigen (hb8 ag) particles vary in their antigenic structure. they are generally found to carry either determinant d or determinant y, but not both. this report describes seven sera which contain australia antigen carrying both d and y on the same particle. | 1975 | 47365 |
| experimental autoimmune encephalomyelitis in mice: immunologic response to mouse spinal cord and myelin basic proteins. | it was confirmed that experimental autoimmune encephalomyelitis eae, could be induced in sjl/j mice with mouse spinal cord homogenate. it was shown that induction of eae in mice was critically dependent on the concentration of pertussis vaccine. the encephalitogen present in mouse brain was the basic protein of myelin. the smaller form of the mouse and rat basic proteins induced eae; thus the mouse like the rat responds to determinants other than the "tryptophan region," which induced eae in gui ... | 1975 | 47366 |
| induction of histamine release and desensitization in human leukocytes. effect of anaphylatoxin. | hog anaphylatoxin (at) in concentrations from 0.5 to 5 mug/ml gives a dose-dependent histamine release from human leukocytes. concentration of 100 mug/ml at give the same high histamine release as 5 mug/ml. this is in contrast to the histamine release obtained with anti-ige or allergen, which give low histamine release with high doses. the histamine release obtained with at is completed in 20 sec and the reaction is temperature- and calcium-dependent. treatment of cells with at in the presence o ... | 1975 | 47368 |
| immunologic studies in hypersensitivity pneumonitis--quantitative precipitins and complement-fixing antibodies in symptomatic and asymptomatic pigeon breeders. | quantitative precipitation and complement fixation tests were performed on sera from a group of patients with hypersensitivity pneumonitis (pigeon breeders' disease). their antibody levels were compared to a selected group of similarly exposed but asymptomatic subjects. the quantity of precipitating antibodies was generally greater in serum from symptomatic breeders, although quantities in the serum from some of the asymptomatic subjects were greater than that seen in ill breeders. complement-fi ... | 1975 | 47374 |
| the uptake of a substituted acridone by rat mast cells in relationship to histamine release: a possible indicator of exocytosis-induced expansion of the plasma membrane. | a substituted acridone, 10-(2-dimethylaminopropyl)-9-acridone hcl (m-129), was taken up by isolated rat peritoneal and pleural mast cells in direct relationship to the degree of selective histamine release induced by compound 48/80. other selective releasing agents, i.e., polymyxin b and anti-rat mast cell serum, also augmented uptake of m-129. augmented uptake of m-129 was inhibited by measures that inhibited selective histamine release, i.e., cold, brief heating of the mast cells, n-ethylmalei ... | 1975 | 47389 |
| immediate hypersensitivity to hog trypsin resulting from industrial exposure. | clinical, immunologic and psysiologic studies were undertaken to establish the role of hog trypsin dust in four cases of occupational asthma and to define the mechanism of this respiratory disease. objective evidence was obtained that these patients, but not their asymptomatic co-workers, were allergic to trypsin, and that this fact accounted for their respiratory symptoms. direct skin testing, passive transfer of ige antibodies, antigen-mediated histamine release from peripheral blood leukocyte ... | 1975 | 47609 |
| antibody to a molecularly-defined antigen confined to a tumour cell surface. | | 1975 | 47617 |
| characterization of spermatozoal auto-, iso- and allo-antigens. | three approaches are utilized to study and characterize spermatozoal antigens. an immunological approach has demonstrated the presence of spermatozoal auto-, iso- and allo-antigens. spermatozoal auto-antigens studies by several authors are able to induce the whole spectrum of immune reactions (delayed hypersensitivity, complement-fixing antibodies and anaphylactic antibodies0 as well as of autoimmune aspermatogenic orchiepididymitis (aiao). different extraction procedures result in various prepa ... | 1975 | 47684 |
| report: endocrine cells in the kidney. | | 1975 | 47693 |
| a possible relationship between delayed hypersensitivity and cell-mediated immunity. | | 1975 | 47728 |
| a contribution to the physiology of the perilymph. part iv: effect of histamine on the cochlear microcirculation. | summary--because of the topography of the perilymphatic vascular network, the cochlear microcirculation is especially sensitive to the effect of vasoactive substances reaching the perilymphatic space. the cochlear microcirculation is mainly controlled humorally, via the activity of the metarterioles and changes in the flow properties of the blood. intracochlear histamine reduces the cochlear circulation even more markedly than after intracarotid administration. no systematic effects of parentera ... | 1975 | 47733 |
| exposure of histone antigenic determinants in chromatin. | the exposure of antigenic determinants of histones present in "native" chromatin was studied by: (1) testing their ability to elicit anti-histone antibodies and (2) measuring their ability to interact with anti-histone sera. to this end, antisera specific to purified histone fractions and to purified rat liver chromatin were elicited in rabbits. the anti-chromatin sera did not react with pure histone fractions and pure histone fractions f2b, f3, f2a1, and f2a2 failed to inhibit the complement fi ... | 1975 | 47758 |
| the beta-adrenoceptors of the lung mediating inhibition of antigen-induced histamine release. | the beta-adrenoceptor stimulants, isoprenaline (ipr), orciprenaline (opr), terbutaline (trb), and itp were studied for effects on antigen-induced release of histamine from guinea-pig lung tissue and for effects on guinea-pig isolated trachea and heart. the order of potency for the agents in the four funct-ons studied were: (a) inhibition of histamine release, ipr greater than opr approximately equal to trb greater than itp equal 0; (b) heart stimulation, chronotropic effect, ipr greater than opr ... | 1975 | 47809 |
| differing patterns of cholesterol accumulation and 3-h-cholesterol influx in areas of the cholesterol-fed pig aorta identified by evans blue dye. | | 1975 | 47812 |
| myxoviruses. | myxoviruses can be divided into 2 groups, orthomyxoviruses and paramyxoviruses. the former comprise the influenza group which is subdivided into types a,b and c. influenza b and c are purely human pathogens but influenza a, which includes a large number of antigenic subtypes, occurs in nature in pigs, horses, birds and man. all influenza a viruses irrespective of origin are chemically, biologically and genetically related. the epidemics which they cause are curious and puzzling and are an import ... | 1975 | 47825 |
| carrier and hapten functions in immune deviation. ii. role of hapten and carrier on the helper functions of the t cells. | the kinetics of haemagglutinating and haemolytic antibody synthesis to the hapten and to the carrier determinants were studied in guinea-pigs injected intravenously with large doses of the carrier, or of the hapten conjugated to an homogous or heterologous protein carrier and subsquently immunized with the hapten-carrier conjugate in freund's complete adjuvant. the animals treated with the heterologous conjugates exhibited enhanced reactions to the hapten and supressed reactions to the carrier, ... | 1975 | 47838 |
| a new method for the measurement of the opsonin titres of antisera to bordetella pertussis. | | 1975 | 47859 |
| the histochemical localization of n-l-arginyl-2-naphthylamine- and n-l valyl-2-naphthylamine-cleaving enzymes in the guinea-pig testis. | | 1975 | 47910 |
| letter: localized hypertrophic neuropathy, intraneural tumour, or chronic nerve entrapment? | | 1975 | 47973 |
| letter: longitudinal sliding of median nerve during hand movements: a contributory factor in entrapment neuropathy? | | 1975 | 47974 |
| letter: green-tobacco sickness. | | 1975 | 48025 |
| development and application of sequence-specific radioimmunoassays for analysis of the metabolism of parathyroid hormone. | | 1975 | 48177 |
| antibodies reactive with cell surface carbohydrates. | normal and immune sera from various animal species were fractionated on columns of sepharose covalently coupled with the glycoprotein fetuin. elution of the material bound to fetuin yielded low but reproducible amounts of protein, ranging from 0.02 to 0.2% of the protein mass of the input sera. this material has been identified by immunoelectrophoresis in agar and by zone electrophoresis on cellulose acetate as immunoglobulin. the ig fractions bound and agglutinated erythrocytes of various speci ... | 1975 | 48249 |
| [rheumatoid factor--rheumatoid factor as an antibody and its relationship to the disease process]. | | 1975 | 48283 |
| [an immunologic study of hyaluronidase of different animal origin]. | studied was the antigenic relatedness of hyaluronidase contained in the semen of breeder animals of homologic and heterologic species. the experiments were carried out by means of the immunodiffusion and the immunoelectrophoretic methods. the results obtained showed that the seminal hyaluronidase of bulls, rams and bucks is antigenically related, and that of stallions, boars and rabbits does not exhibit antigenic relatedness. stallion semen is closely related antigenically with the above-mention ... | 1975 | 48305 |
| ir genes and antigen recognition. | | 1975 | 48355 |
| inhibition of immediate hypersensitivity reactions in laboratory animals by a phenanthroline salt (ici 74,917). | 1. the activity of a new anti-allergic compound, i.c.i. 74,917, has been studied in the rat, mouse and guinea-pig. 2. following intravenous administration, i.c.i. 74,917 inhibits in a dose-dependent manner passive cutaneous anaphylaxis induced in rats and mice by heat-labile homocytotropic antibody. in rats, its potency is approximately 300 times that of disodium cromoglycate. 3. to achieve maximal inhibition, it is necessary to administer i.c.i. 74,917 at the same time as antigenic challenge; d ... | 1975 | 48393 |
| macrophage activation by lymphocyte mediators. | | 1975 | 48483 |
| surface immunoglobulin of guinea-pig leukaemic lymphocytes. | the surface immunoglobulin of the transplantable l2c leukaemia of strain 2 guinea-pigs has been investigated. the immunoglobulin is seen to be synthesized when the cells are maintained in culture, indicating its intrinsic origin. immunolabelling of the cell surface and immunochemical study of the fab released by limited surface proteolysis indicate the presence of immunoglobulin of class igm. igg and free light chains were not detected, and there is unlikely to be an appreciable amount of immuno ... | 1975 | 48498 |
| release of histamine from rat mast cells by the complement peptides c3a and c5a. | suspensions of rat mast cells were used to study the histamine-releasing actions of anaphylatoxins c3a and c5a in vitro. the peptides, derived from human or porcine complement proteins c3 and c5, were less potent than 48/80 but more potent than bradykinin in stimulating release of histamine from mast cells. the pattern of release resembled that of the anaphylactic release action, e.g. release was limited to less than 30 per cent of the cell histamine, the reaction was calcium-dependent and was p ... | 1975 | 48505 |
| inuction of immunological tolerance in immunoglobulin e b lymphocytes in rats. | immunological tolerance has been induced in 2,4-dinitrophenyl (dnp)-specific bone marrow-derived or b lymphocytes of the ige and igg antibody classes by treatment of rats with the dnp derivative of d-amino acid copolymer, d-glutamic acid, d-lysine (d-gl). the tolerant state is manifested as an inability of treated rats to produce serum anti-dnp antibodies and the failure of peritoneal cells from tolerant animals to release histamine following in vitro antigen challenge. the implications of these ... | 1975 | 48523 |
| [detection of amylolytic and proteolytic isoenzymes in biological fluids by the method of disc-electrophoresis in polyacrylamide gel]. | | 1975 | 48589 |
| [study of the virulence of shigella in the reaction with tripaflavin with the aid of kertoconjunctival test]. | | 1975 | 48599 |
| letter: 1-18 corticotrophin and allergy to tetracosactrin-depot. | | 1975 | 48646 |
| editorial: fluorocarbon aerosol propellants. | | 1975 | 48735 |
| letter: transplantation and burns. | | 1975 | 48805 |
| growth-hormone release-inhibiting hormone in gastrointestinal and pancreatic d cells. | the normal pancreatic d cell has been well recognised since 1931, but its secretory product has not been identified with certainty. combined immunocytochemical methods, and histological methods for endocrine granules, have demonstrated that it contains a product with g.h.-r.i.h.-like immunoreactivity. g.h.-r.i.h. has also been localised in the upper gastrointestinal tract, tentatively in the morphologically similar d cell of the wiesbaden classification. the potent actions of this peptide on pan ... | 1975 | 48838 |
| porcine streptococci causing meningitis and septicaemia in man. | from 1968 to 1974 seven cases of septicaemia and purulent meningitis caused by porcine streptococci of the lancefield group r and three cases caused by similar streptococci lacking r antigen occurred in the netherlands. bacteria isolated from all ten patients shared the characteristics of a biochemically well defined bacterial species (provisionally called streptococcus subacidus) which until 1968 was only found in pigs. nine of the ten patients had had intensive contact with live or slaughtered ... | 1975 | 48908 |
| effect of intravenous b.c.g. in guineapigs and pertinence to cancer immunotherapy in man. | intravenous injection of heat-killed or irradiated b.c.g into tuberculin-positive guineapigs produced macroscopic lesions in the lung when examined 10 days or 4 or 6 weeks later. microscopically, granulomas typical of a delayed hypersensitivity reaction were seen. intravenous b.c.g. in normal guineapigs did not produce lesions. at equivalent doses to the killed vaccine, viable vaccine caused only mild lesions. liver lesions were also found on early examination but by 4 weeks had almost resolved. ... | 1975 | 48944 |
| group-specific component (gc) proteins bind vitamin d and 25-hydroxyvitamin d. | group-specific component (gc) proteins are human plasma proteins for which a worldwide polymorphism exists. as yet no functional role has been assigned this protein. we show that the products of both gc alleles, proteins gc 1 and gc 2 (distinguished electrophoretically), bind substantial quantities of vitamin d and 25-hydroxyvitamin d. three lines of evidence are reported: (1) polyacrylamide gel electrophoresis and autoradiography of serum labeled with (14-c)vitamin d3 revealed patterns of radio ... | 1975 | 49052 |
| [some indices of the reactivity of the macroorganism in the use of acs in the overall therapy of pulmonary tuberculosis]. | | 1975 | 49129 |
| [immunodepressive properties of certain components of placental blood]. | | 1975 | 49152 |
| validity of plasma factor in schizophrenia as measured by tryptophan uptake. | the uptake of l-tryptophan by chicken erythrocytes is stimulated by the presence of human plasma; however, there is no difference between the stimulation caused by plasmas of normal, chronic hospitalized nonschizophrenic, or chronic hospitalized schizophrenic subjects. human plasma in general contains a complement-dependent antibody which reacts with an antigen present in the membrane of chicken erythrocytes resulting in hemolysis. hemolysis can be maintained at a constant level by certain conce ... | 1975 | 49200 |
| allergic encephalomyelitis: modification of the response by synthetic membrane structures containing bovine myelin basic protein and cerebroside. | | 1975 | 49214 |
| specific fetal serum proteins of thirteen mammalian species. | | 1975 | 49249 |
| isolation of mammalian type c rna virus cross-reactive antigen and antibody by immuno-affinity chromatography. | | 1975 | 49291 |
| multiple specificities of mammalian blood group substances comparatively studied with human isoagglutinins and fractionated anti-h lectins. | purified blood group-active substances derived from different pig, horse, baboon, rhesus monkey and human tissues were quantitatively studied for their haemagglutination inhibiting potency with: (1) human igm anti-a and anti-b; (2) human anti-lea and anti-leb; (3) ulex europaeus extracts separated into lectin fractions with respective l-fucose-inhibitable ('anti-hf') and chitobiose-cellobiose-inhibitable ('anti-hc') combining sites. irrespective of species origin, a and b blood group activity pe ... | 1975 | 49294 |
| human antibodies to bovine alpha-globulin. | antibodies to bovine gamma-globulin (anti-bgg antibodies) were detectable by a radio-immunoassay in 70% of healthy blood donors but, generally, the titres were low. significantly increased concentrations of anti-bgg antibodies were found in patients lacking iga but not in patients with allergic disorders. the anti-bgg antibodies were shown to give rise to falsely high ige values in the radio-immunosorbent test for ige determination (rist) when a sheep anti-ige antiserum was used. furthermore, fa ... | 1975 | 49305 |
| molecular mechanism of t and b lymphocyte triggering. | | 1975 | 49320 |
| on axoplasmic flow. | | 1975 | 49329 |
| the use of glyoxylic acid for the fluorescence histochemical demonstration of peripheral stores of noradrenaline and 5-hydroxytryptamine in whole mounts. | the reactions of glyoxylic acid with peripheral stores of noradrenaline and 5-hydroxytryptamine to provide a fluorescence histochemical method for their localization have been investigated. incubation in glyoxylic acid, followed by drying and heating of whole mount preparations gives an intense and well localized reaction. for incubation, a concentration of 2% glyoxylic acid, buffered to ph 7 at room temperature for 30 minutes gives ideal results. the method is equally good if the ph is varied i ... | 1975 | 49335 |
| serodiagnostic application of immunohistoperoxidase reactions on antigen-coupled agarose beads. | agarose beads to which antigens were covalently bound were subjected to indirect immunohistoperoxidase procedures. for detection and titration of serum antibodies against bovine gamma globulin (bgg) this method appeared to be specific and sensitive. one advantage is that no special instruments are needed. as an example of diagnostic applicability the system was successfully used for demonstration of antibodies in human serum containing antibodies against the trematode schistosoma mansoni. at the ... | 1975 | 49378 |
| induction of t-lymphocyte responses to a small molecular weight antigen. iii. t-t cell interactions to determinants linked together: suppression vs. enhancement. | the experiments presented in this paper demonstrate the existence of t-t cell interactions in responses to azobenzenearsonate (aba)-protein conjugates, and also make the point that the spectrum of t-cell regulation from facilitation (i.e., help) at one end to suppression at the other, which has been well documented in t-b cell interactions, is also followed in t-cell regulation of other t lymphocytes. the data extend the activity of aba-specific suppressor cells, which were shown to specifically ... | 1975 | 49385 |
| type c virus production by a continuous line of pig oviduct cells (pft). | late passages of the pft porcine oviduct cell line spontaneously release a typical type c virus antigenically related to the type c virus released from the pk(15) porcine kidney cell line. the pft virus was non-infective for a large variety of cell lines. type c virus can also be induced by brdu treatment from earlier passages of the pft cell line. | 1975 | 49396 |
| interspecies antigenic determinants of the reverse transcriptases and p30 proteins of mammalian type c viruses. | the major internal structural proteins (p30) of type c viruses isolated from several mammalian species were studied by radioimmunoprecipitation and competitive radioimmunoassays. three antigenically distinguishable sets of interspecies determinants could be demonstrated by both methods. one set of determinants shared by viruses of rodent origin (mouse and rat) can be detected readily in feline leukemia viruses but not in other type c viral groups. the p30 proteins of murine viruses also contain ... | 1975 | 49443 |
| [current problems of active substance research. 7. use of animal cell- and tissue cultures for comprehending and explanation of biological effect of substances. cultivated liver cells as test objects for active substances]. | | 1975 | 49898 |
| a pattern in the development of agglutinogenic antigens of cyclically transmitted isolates of trypanosoma gambiense. | three isolates of t. gambiense, belonging to 2 serologically different substrains of the species, were cyclically transmitted to rabbits and monkeys by g. morsitans or g. tachinoides. sera collected from the infected animals were tested for agglutinating antibodies to 17 different serotype antigens prepared from rodent-adapted isolates of t. gambiense. the first antibodies detected in sera from all of the animals agglutinated the same serotype antigen l2, indicating that it possibly consisted of ... | 1975 | 49958 |
| [the differenziation of swine-mycoplasma by acrylamide-gel-electrophoresis (author's transl)]. | the strains of swine mycoplasma (tab. 1) were grown aerobically in whittlestone's medium containing swine serum for 4-5 days at 37 degrees c. after centrifugation and washing they were freeze-dried and extracted with a phenol-acetic acid-water solution (4:2:1) (1 ml/5 mg mycoplasma dru weight) at 4 degrees c over 48 h. after centrifugation 2 volumes of the supernatant were mixed with 1 volume of a 40% sucrose solution in 35% acetic acid. 0,15 or 0,2 ml of which were added to the acrylamide-gel ( ... | 1975 | 49991 |
| effects of colchicine and vinblastine on axonal transport and transmitter release in sympathetic nerves. | | 1975 | 50036 |
| binding of 14-c-salicylic acid and 14-c-pentobarbital to plasma proteins of several species during the perinatal period. | the fraction binding of 14-c-salicylic acid and 14-c-pentobarbital was studied as a function of age in plasma of the pig, dog, goat and human. the pig exhibited an unusual degree of hypoalbuminemia and low fractional binding of salicylic acid at birth. albumin levels and the percent binding of the salicylic acid were not significantly lower in newborn plasma than in adult plasma of the other species studied. the pig was also exceptional in that the high fractional binding of pentobarbital observ ... | 1975 | 50090 |
| the antigenic interrelations of some mammalian igg subclasses detected with cross-reacting fowl antisera to human and mouse igg-fc. | two fowl antisera to plyclonal human igg and one to a mouse igg1 myeloma protein, after absorption either with homologous f(ab)2 alone, or also with myeloma proteins of known subclasses, cross-reacted in immunoelectrophoresis or immunodiffision withthe igg of many mammalian species. often more than one igg precipitation line was formed in the corss-reacting systems. by testing isolated igg1 and igg2 fractions from mouse, rat, guinea-pig, bovine and dog sera, it was shown that the distinct arcs ... | 1975 | 50284 |
| drug inhibition of anaphylactic histamine release from peritoneal cells of rats infected with toxocara canis. | an experimental model for studying anaphylactic histamine release from rat peritoneal cells in vitro is described. rats were sensitized by feeding the infective eggs of ttoxocara canis, the antigen being prepared from the infective eggs. the characteristics of histamine release are comparable with those found for other anaphylactic systems. addition of 0.2% inactivated normal rat serum to the incubation media reduced the spontaneous histamine release from 11.5 +/- 1.9% to 3.1 +/- 1.6% (mean +/- ... | 1975 | 50289 |
| the intracellular neutral sh-dependent protease associated with inflammatory reactions. | | 1975 | 50301 |
| background staining and sensitivity of the unlabelled antibody-enzyme (pap) method. comparison with the peroxidase labelled antibody sandwich method using formalin fixed paraffin embedded material. | the pap procedure was compared with the peroxidase labelled antibody sandwich method and was found to be at least 20 times more sensitive. background staining was reduced by the addition of normal swine serum to all the immune sera or by pretreating sections with it at the beginning of either method. the pap procedure could be effectively reduced to a period of 1 hour or less. | 1975 | 50308 |
| regulation of mif by a factor in human serum. | | 1975 | 50355 |
| suppressor cell control of unresponsiveness to experimental allergic encephalomyelitis. | lymph node cells (lnc) from lewis rats rendered unresponsive to experimental allergic encephalomyelitis (eae) by pretreatment with myelin basic protein markedly suppressed clinical (but not histologic) eae in normal recipients later challenged with an encephalitogenic emulsion. unresponsiveness was immunologically specific, and required viable lnc; serum transfer was ineffective. these findings suggest that suppressor cells exert control over this autoimmune disease. | 1975 | 50370 |