| alterations in ascaris suum larval burdens, eosinophil numbers, and lysophospholipase activity associated with low levels of dietary iron. | the effect of various amounts of dietary iron on the immune response was investigated using balb/canncr/br mice infected with ascaris suum. changes in numbers of larvae, numbers of eosinophils, and levels of lysophospholipase (lpl) activity in lung or liver tissues were analyzed from nonimmune and immunized mice at 2 and 7 days postinfection (pi). various iron diets did not influence the numbers of tissue larvae, eosinophils, or the lpl activity in lungs or livers of nonimmunized mice at various ... | 1989 | 2926596 |
| effect of calcium antagonist gallopamil on antigen-induced early and late bronchoconstrictor responses in allergic sheep. | calcium channel blockers have been shown to modify acute antigen-induced bronchoconstriction in allergic animals and in some human subjects with bronchial asthma; however, the effects of calcium antagonists on late-phase bronchial responses are not known. in this investigation, we studied the effects of calcium antagonist gallopamil on antigen-induced early- and late-phase bronchial responses in sheep allergic to ascaris suum antigen. in 11 unsedated sheep, specific lung resistance (srl) was mea ... | 1989 | 2930069 |
| survey of internal parasites in western australian pig herds. 1. prevalence. | between september 1982 and march 1984, 101 western australian piggeries with 15 or more sows were surveyed to determine the prevalence of internal parasites and examine the relationship between parasitism and management practices. faecal samples were collected from 20 pigs in 4 age groups in randomly selected piggeries, and examined for the presence of eggs of helminth parasites and protozoan cysts. evidence of nematode parasites was found in 79% of piggeries. sows were more commonly affected th ... | 1989 | 2930392 |
| fc and c3b receptor changes on mouse peritoneal leucocytes following stimulation with an extract of ascaris suum. | changes in igg-fc and igm-c3b receptors of mouse peritoneal leucocytes after in vivo stimulation with ascaris suum adult extract were studied. erythrocyte igg-fc rosettes were found to initially be decreased with a. suum stimulated neutrophils, eosinophils and macrophages in comparison with nonstimulated cells. igg-fc rosettes then increased with time and remained high for a 60 min exposure time. igm-c3b rosette formation was lower with a. suum stimulated leucocytes throughout 60 min of exposure ... | 1985 | 2932876 |
| down-regulation of immune responses to inhaled antigen: studies on the mechanism of induced suppression. | repeated exposure of rats to an aerosol of ovalbumin (ova) or its dinitrophenylated derivative (dnp-ova) induced carrier-specific tolerance to subsequent challenge with the same haptenated antigen. following parenteral challenge with dnp-ova, both anti-dnp and anti-ova ige titres were reduced relative to controls, whereas anti-dnp responses following challenge with dnp-ascaris were normal. stimulation of tolerant rats with ova, together with the polyclonal b-cell mitogen lps, restored their capa ... | 1985 | 2934319 |
| studies on a new antiallergic pyridinecarboxamide ta-5707f and its sodium salt (ta-5707). i. inhibition of ige-induced passive cutaneous anaphylaxis (pca) in rats. | effects of ta-5707f [6-methyl-n-(1h-tetrazol-5-yl)-2-pyridinecarboxamide] and its sodium salt (ta-5707) on ige-induced homologous pca in rats were investigated. 1. both ta-5707 and ta-5707f were found to be orally effective inhibitors of rat pca. maximum activity by oral administration was obtained when they were administered 5 min before the challenge. their id50's under these conditions were both approximately 1 mg/kg. administration 5 min after the challenge was no longer effective. 2. ta-570 ... | 1986 | 2937947 |
| properties and localization of chitin synthase in ascaris suum eggs. | investigations were carried out into the properties and localization of chitin synthase (ec 2.4.1.16) in connection with the formation of ascaris suum egg-shells. the chitin synthase of eggs exhibited properties very similar to those found in other chitin-synthesizing organisms. it was stimulated by the presence of atp, glcnac and mg2+ with maximal activity at ph 7.0. in the phase of active chitin synthesis, during formation of the primary shell layers, chitin synthase was bound to the structure ... | 1986 | 2938060 |
| protective immunity to ascaris suum: analysis of swine peripheral blood cell subsets using monoclonal antibodies and flow cytometry. | outbred domestic swine or sla inbred miniature swine were exposed to ascaris suum either naturally on contaminated lots or by inoculation with uv-irradiated attenuated eggs. both inbred and outbred swine developed virtually complete protection to a challenge of 10 000 eggs after natural exposure, but inbred swine were less resistant than outbred swine after uv-egg exposure. flow cytometric analysis of peripheral blood mononuclear cells from these animals, performed to determine changes in cell s ... | 1986 | 2939618 |
| experimental larval ascaridosis (ascaris suum goetze, 1782) in white mice. i. histochemical studies on the liver. | | 1985 | 2940825 |
| proteolytic activity of ovicidal fungi. | | 1985 | 2949532 |
| correlation between auto-dehelminthizing ability of the soil and occurrence of geohelminth eggs. | | 1985 | 2949533 |
| correlation between hysteresis and allosteric properties for phosphofructokinase from ascaris suum. | the ascaris suum phosphofructokinase exhibits hysteretic transitions in the time course for fructose 6-phosphate (f6p) phosphorylation in addition to allosteric properties when assayed at ph values below 8. conditions that enhance hysteretic changes also enhance cooperative interactions and thus there appears to be a link between hysteresis and cooperativity. initiation of reaction with either f6p or phosphofructokinase results in a pronounced lag, while initiation of the reaction with mgatp res ... | 1987 | 2958457 |
| reaction of ascaris suum phosphofructokinase with diethylpyrocarbonate. inactivation and desensitization to allosteric modulation. | reaction of the phosphofructokinase from ascaris suum with the reagent, diethylpyrocarbonate (depc), results in the loss of enzymatic activity. treatment of the inactivated enzyme with hydroxylamine brings about the recovery of almost 80% of the original activity suggesting that the modified residues are histidines. further evidence for the modification of histidines is that concomitant with the loss of activity, there is a change in a242 nm that corresponds to the derivatization of 5-6 histidin ... | 1987 | 2958458 |
| kinetic mechanism of ascaris suum phosphofructokinase desensitized to allosteric modulation by diethylpyrocarbonate modification. | the kinetic mechanism of phosphofructokinase has been determined at ph 8 for native enzyme and ph 6.8 for an enzyme desensitized to allosteric modulation by diethylpyrocarbonate modification. in both cases, the mechanism is predominantly steady state ordered with mgatp binding first in the direction of fructose 6-phosphate (f6p) phosphorylation and rapid equilibrium random in the direction of mgadp phosphorylation. this is a unique kinetic mechanism for a phosphofructokinase. product inhibition ... | 1987 | 2958459 |
| a profile of ascariasis morbidity in rangoon children's hospital, burma. | the rangoon children's hospital (rch) inpatient summary returns from hospital wards from 1981 to 1983 and the surgical operation registers from 1983 to 1984 were studied and analysed in relation to ascariasis morbidity. the majority (57.7%) of clinical manifestations of ascariasis responsible for hospital admission were due to intestinal obstruction, the next most common manifestation was intestinal colic (33.0%). mean ages for the two conditions were 5.9 years and 7.6 years, respectively. hospi ... | 1987 | 2958641 |
| inhibition of histamine and allergen skin wheal by cetirizine in four animal species. | histamine-induced skin reactions were studied in four animal species. cetirizine displayed very potent oral antihistaminic activity in this model. in rats, the dose that reduced the reaction by 50% in comparison to controls was 4.2 mumol/kg and between 0.1 and 0.4 mumol/kg for the other species (mice, guinea pigs, and dogs). cetirizine also inhibited the immediate hypersensitivity reaction induced by ascaris extract in dogs. by the oral route, cetirizine had a more potent and longer acting activ ... | 1987 | 2959177 |
| reinfection of people with ascaris lumbricoides following single, 6-month and 12-month interval mass chemotherapy in okpo village, rural burma. | a longitudinal study on reinfection with ascaris lumbricoides was continued on a random sample of 50% of the infected population following a horizontal cross-sectional study in okpo village, near rangoon. the study sample was again randomly divided into two subsamples, the six-month interval worming group and the 12-month interval worming group. microscopic examination of stool for ascaris eggs on the 7th day and 30th day, combined with counting eggs and worming with levamisole, were carried out ... | 1987 | 2965438 |
| losses to internal parasites in swine production. | experimental infections of pigs with strongyloides ransomi, stephanurus dentatus, ascaris suum, oesophagostomum spp. or trichuris suis at increasing levels generally decrease daily gain and increase feed to gain ratio linearly. at lower, subclinical levels of infection, the feed to gain ratio typically is increased 3% to 6% by an infection. based on low-level experimental infections with a. suum, the economic loss to producers in the u.s. from increased feed to gain ratio is estimated at $155 mi ... | 1988 | 2969380 |
| auxotrophic mutants of ovicidal fungus penicillium lanosum. | | 1988 | 2977919 |
| the role of some cyclorrhaphan flies as carriers of human helminths in malaysia. | the role of some adult flies (diptera: cyclorrhapha) as carriers of helminth parasites of man was studied at four sites in malaysia: a refuse dump, where no helminth-positive flies were detected, and in three peri-domestic situations where four species of flies carried up to three types of nematodes. the dominant fly species chrysomya megacephala (fabricius) carried eggs of the roundworm ascaris lumbricoides l., the pinworm trichuris trichiura (l.) and hookworm on the adult external body surface ... | 1988 | 2980156 |
| ascaris lumbricoides: characterization of the collagenous components of the adult cuticle. | the proteins of the cuticle of adult ascaris lumbricoides suum were characterized with respect to heterogeneity, glycosylation, and susceptibility to collagenase. pepsin digestion of intact cuticles was used to determine the extent of stable triple-helical structures of the cuticular components. with sodium dodecyl sulfate-poly-acrylamide gel electrophoresis, it was shown that treatment of purified cuticles with beta-mercaptoethanol released three components (99, 90, and 68 kda) which comprise 9 ... | 1985 | 2982639 |
| dolichol kinase in ascaris suum and onchocerca volvulus. | dolichol kinase was demonstrated in the microsomal fraction of ascaris suum and onchocerca volvulus. the enzyme from nematodes exhibited specificity for ctp as phosphoryl donor and was found to be inhibited by the reaction product cdp. enzyme activity was optimal at ph 7.4, in the temperature range between 30 degrees and 37 degrees c, and in the presence of 0.5% triton x-100. in addition, the enzyme was found to depend on divalent cations for activity; mg2+ being more effective than mn2+ and ca2 ... | 1985 | 2985982 |
| the effect of nedocromil sodium and sodium cromoglycate on antigen-induced bronchoconstriction in the ascaris-sensitive monkey. | nedocromil sodium inhibited the bronchoconstriction caused by antigen challenge in ascaris-sensitive monkeys and in addition it prevented the release of histamine from mast cells lavaged from sensitive monkeys. sodium cromoglycate was relatively inactive in both these systems. it is suggested that nedocromil sodium can stabilize both mucosal and connective tissue mast cells and may represent a new type of drug. | 1985 | 2992657 |
| pulmonary responses to exogenous leukotrienes. | | 1985 | 2999823 |
| suppression of pulmonary and systemic vascular histamine h2-receptors in allergic sheep. | we have previously demonstrated a depression of airway h2-receptor function in sheep allergic to ascaris suum antigen. to investigate whether this is a generalized defect, we studied the h1- and h2- histamine receptor functions in the pulmonary and systemic circulations of allergic and nonallergic sheep. pulmonary arterial pressure, and cardiac output were measured for calculation of pulmonary vascular resistance (pvr) and systemic vascular resistance (svr) before and immediately after a rapid i ... | 1986 | 3007427 |
| structural analysis of the calcium-binding protein calmodulin from ascaris suum obliquely striated muscle. | calmodulin was purified from the obliquely striated skeletal muscle of ascaris suum. the calmodulin had a molecular weight of 16,400 and the amino acid composition indicated it is highly similar to other purified calmodulins, showing insignificant variation in 12 of 17 residues. in the residues that showed variation, a trend towards conservative substitution was observed. spectrophotometric absorption maxima of 276 nm and 283 nm were observed. a molar absorption coefficient of 7,800 was calculat ... | 1986 | 3016225 |
| prevention of canine anaphylaxis with isoproterenol. | sympathomimetic agents are used to reverse the cardiovascular and respiratory abnormalities that are associated with anaphylactic shock. in addition, in vitro studies have demonstrated that beta-adrenergic stimulation modulates ige-mediated release of chemical mediators. to separately evaluate the ability of isoproterenol, a beta-adrenergic agonist, to prevent mediator release and anaphylactic shock, as well as to reverse the cardiopulmonary manifestations of systemic anaphylaxis, we administere ... | 1986 | 3017167 |
| characterization of inverted repeated sequences in ascaris nuclear dna. | the inverted repeated sequences of the chromatin-eliminating nematode ascaris lumbricoides var. suum have been examined by electron microscopy and by hydroxyapatite chromatography, both in the germ-line and in the somatic dna. 38% of the inverted repeats of the germ-line dna analysed in the electron microscope have a single-stranded loop, in comparison to about 50% of looped structures in the somatic dna. the loops are on average 2.3 x 10(3) base pairs (bp) long. the rest of the foldback dna con ... | 1986 | 3019693 |
| reversible activation and inactivation of phosphofructokinase from ascaris suum by the action of tissue-homologous protein phosphorylating and dephosphorylating enzymes. | in the presence of atp-mg2+, purified phosphofructokinase from ascaris suum muscle was effectively phosphorylated and activated in vitro by a protein kinase purified from the same tissue. both effects were reversed by the action of a purified protein phosphatase from the same tissue. the findings suggest the presence of a highly potent interconversion mechanism for phosphofructokinase in the muscle of the parasitic nematode. | 1986 | 3021124 |
| monkeys infected with ascaris suum (a new in vivo model of airway disease): protective effect of nedocromil sodium and sodium cromoglycate against bronchial antigen challenge. | the experimental infection of macaca arctoides monkeys with the nematode parasite ascaris suum induces an ige-mediated immediate hypersensitivity reaction similar to that seen in naturally sensitive animals. in the present study, the effects of inhalation of ascaris suum antigen on lung function in infected monkeys were investigated, together with the effects of aerosolized nedocromil sodium and sodium cromoglycate (each 4 mg) given 5 min prior to antigen challenge. ascaris suum-infected macaca ... | 1986 | 3021491 |
| ascaris suum phosphofructokinase. phosphorylation by protein kinase and sequence of the phosphopeptide. | phosphorylation of the ascarid phosphofructokinase with the catalytic subunit of beef heart cyclic amp-dependent protein kinase results in the incorporation of 1 mol of p/mol of subunit. accompanying the phosphorylation there is a 3-4-fold increase in catalytic activity when measured at ph 6.8 with inhibitory levels of atp. studies on the effect of phosphorylation on the atp saturation curve demonstrated that phosphorylation decreased the inhibitory action of atp. the apparent km of the catalyti ... | 1987 | 3025208 |
| ascaris dna topoisomerase i binds preferentially to the germ-line- limited dna. | | 1986 | 3029600 |
| the gamma-aminobutyric acid receptor of ascaris as a target for anthelmintics. | | 1987 | 3030844 |
| infectivity with human ascariasis in ibadan oyo state, nigeria. | some of the common foodstuffs that assist in the transmission of ascariasis in ibadan have been determined. the frequency of recovery of ascaris ova in the different food samples examined varied from 9.6% in tomatoes to 25.6% in lettuce leaves. the indiscriminate pollution of ibadan environment with human faeces enables the foodstuff consumed raw to serve as effective sources of heavy ascaris infection which lead to other medical complications. the strategies for the control of ascaris infection ... | 1986 | 3031965 |
| ascaris lumbricoides as a vehicle of bacterial infections. | investigations were conducted on microflora on the body surface and internal organs of adult ascaris lumbricoides. the investigations involved examination of wet faecal preparations of over 100 pupils using light microscope. the body surface of the adult ascaris worms was cultured on selective media. adult worms were dissected and different parts of the worms' gut were cultured for isolation of micro-organisms. the results of the bacteriological examination of the body surface of a. lumbricoides ... | 1986 | 3031966 |
| biochemical changes during the aerobic-anaerobic transition in ascaris suum larvae. | ascaris suum l3 larvae isolated from rabbit lungs undergo the third ecdysis to l4 larvae after 3 days in culture under a gas phase of 85% n2/10% co2/5% o2. the l3 larvae contain substantial malic enzyme activity and are capable of producing small amounts of the reduced organic acids characteristic of the fermentative pathways which operate in the adult. however, only a small portion of the total carbon utilized is accounted for by these reduced acids and their motility is cyanide-sensitive, sugg ... | 1987 | 3033496 |
| ascaris suum: partial isolation and characterization of hypodermis from the adult female. | a method was developed to remove the muscle from body wall strips of adult female ascaris suum resulting in a hypodermis cuticle preparation. optimum treatment for obtaining the hypodermis cuticle was a 15 min incubation with trypsin (2.0 mg/ml) at room temperature, followed by mechanical removal of the muscle. the hypodermis cuticle prepared in this manner incorporated radiolabeled amino acids into cuticular and hypodermal proteins; incorporation was inhibited by protein synthesis inhibitors. c ... | 1987 | 3034657 |
| parasitic pneumonia. | with few exceptions, the parasitic pneumonias most commonly encountered in the western hemisphere are diseases of compromised hosts; patients with aids are at particular risk. pneumocystis carinii pneumonia occurs eventually in 80% of aids patients; bronchoalveolar lavage is quite sensitive in establishing this diagnosis. toxoplasma gondii pneumonia, seen most often in the patient with aids, is characterized by multisystem involvement. strongyloides stercoralis infection is endemic in the southe ... | 1988 | 3041517 |
| [detection of ige antibodies to ascarid antigens by a chemiluminescence method]. | | 1988 | 3045512 |
| efficacy of piperazine dihydrochloride against ascaris suum and oesophagostomum species in naturally infected pigs. | a controlled trial was performed to evaluate the efficacy of piperazine dihydrochloride in a new granular formulation (ascarex d) against naturally occurring infections with ascaris suum, oesophagostomum dentatum and o quadrispinulatum. treatment effects were estimated on the basis of parasites recoverable from the intestinal contents. given orally at 200 mg per kg body weight the compound showed an efficacy of 99 to 100 per cent against a suum and the nodular worms. egg excretion of the respect ... | 1988 | 3046112 |
| immunocytochemical demonstration of a neuropeptide in ascaris suum (nematoda) using an antiserum to fmrfamide. | a fmrfamide-like peptide has been detected in the nematode ascaris suum, using the peroxidase-anti-peroxidase (pap) immunocytochemical technique. positive reactions were obtained in both the central nervous system and the peripheral nervous system of the worm, the strongest reactions being in the anterior nerve ring, the cephalic papillary ganglia, the lateral ganglia and the dorso-rectal ganglion. immunoreactivity was observed along the length of the main nerve cords of the worm and, to a lesse ... | 1988 | 3050818 |
| the secreted and somatic antigens of the third stage larva of anisakis simplex, and antigenic relationship with ascaris suum, ascaris lumbricoides, and toxocara canis. | the in vitro-released 'excretory/secretory' (es) and somatic antigens of the third stage (infective) larva of anisakis simplex were characterised by radioiodination, immunoprecipitation, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. oral infection with the parasite elicited a substantial igg antibody response to es in infected rabbits and humans, with a minimal response to somatic materials. serial serum sampling in experimental infection showed that there was a sequential reco ... | 1988 | 3054540 |
| the sonographic signs of the peritoneal pseudocyst obstructing the ventriculo-peritoneal shunt in children. | the installation of the ventriculo-peritoneal shunt (vps) is on the rise in the pediatric age. its obstruction is a major cause for further deterioration of the already present hydrocephalus. distal vps obstruction may be due to a pseudocyst, an infected pseudocyst or an abscess-formation within the peritoneal cavity. these pathologies are identified through an abdominal radiograph and ultrasonography. the sonographic signs are described and its differential diagnosis is discussed in relation to ... | 1988 | 3062486 |
| murine delayed type hypersensitivity is suppressed by ascaris suum extract. | we studied the suppressive effect of an ascaris suum extract on delayed-type hypersensitivity (dth) reactions to ovalbumin (ova) and to bacillus calmette-guerin (bcg). the ability of mice to develop dth reactions to both antigens was suppressed when an immunizing dose of the antigen was given subcutaneously together with the ascaris extract. partial or complete suppression of the response to ova was obtained by the use of 400 or 1000 micrograms of ascaris extract, respectively. the response to b ... | 1988 | 3067811 |
| detection and localization of actin in toxoplasma gondii. | the immunological cross-reactivity of the protein from whole-cell extracts of toxoplasma gondii with antisera against actins from chicken-gizzard smooth muscle and from ascaris body-wall muscle was demonstrated by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis (sds-page) and immunofluorescence studies. a protein of about 42 kda, with the same mobility as that of rabbit smooth-muscle actin in sds-page showed cross-reactivity with anti-actin antisera on the electroblotted nitrocell ... | 1988 | 3070541 |
| the polyamine metabolism of filarial worms as chemotherapeutic target. | parasite-specific putrescine-n-acetyltransferase and polyamine oxidase, both involved in the reversed pathway of polyamine metabolism, were demonstrated for ascaris suum and onchocerca volvulus. berenil-treatment was found to be correlated with accumulation of polyamines, especially spermine, obviously due to blockaded polyamine interconversion. furthermore it was shown that added spermine to the culture medium led to the death of worms. these specificities might be exploited for chemotherapy of ... | 1988 | 3076347 |
| ascaris suum: immunoperoxidase and fluorescent probe analysis of host proteases and parasite proteinase inhibitors in developing eggs and second stage larvae. | live ascaris suum females were incubated in medium containing chymotrypsin liganded to fluorescein-5-isothiocyanate, and eggs in the parasite's genital tract took up the probe and fluoresced. eggs passed by these worms into the medium containing fluorescent probe retained their fluorescence in formaldehyde-saline and by 65 days had developed into second stage infective larvae. eggs passed naturally by untreated worms were incubated in media containing fluorescent probes and all of the eggs expos ... | 1986 | 3082662 |
| comparative histochemical studies of glycosidase activity in some helminths. | comparative histochemical studies of glycosidase activity were carried out in clonorchis sinensis, eurytrema pancreaticum, fasciola hepatica, dipylidium caninum, hymenolepis nana, ascaris suum, toxocara canis, ancylostoma caninum, trichuris vulpis and dirofilaria immitis. the enzymes examined were: n-acetyl-beta-glucosaminidase (ec 3.2.1.30), beta-glucuronidase (ec 3.2.1.31) and beta-galactosidase (ec 3.2.1.23). there were variations in enzyme distribution and intensity among the species and als ... | 1986 | 3084622 |
| alternative modes of action of sodium cromoglycate. | | 1986 | 3092596 |
| indomethacin and fpl-57231 inhibit antigen-induced airway hyperresponsiveness in sheep. | we compared the development of antigen-induced airway hyperresponsiveness (ahr) 24 h after challenge with ascaris suum antigen in allergic sheep with acute (n = 7) and with dual (n = 7) airway responses and then attempted to modify this ahr. cholinergic airway responsiveness was determined by measuring the carbachol dose required to increase specific lung resistance (srl) 150% (i.e., pc150). subsequently the sheep were challenged with antigen and srl was measured at predetermined times to docume ... | 1986 | 3093455 |
| pharmacological characterization of airway smooth muscle responses to antigen in ascaris-sensitive dogs. | the dog model of ascaris airway sensitivity was chosen because of its frequency and its immunologic similarity to the human atopic asthmatic state. we studied the mediators of the antigen-induced airway response in vitro and the alterations in the in vivo and in vitro responsiveness to spasmogens evoked by antigen challenge. a myogenic basis of altered reactivity was suggested by the following: tetrodotoxin-insensitive spontaneous active tone; phasic contractions of airway smooth muscle; and res ... | 1986 | 3098393 |
| characterization and immunochemistry of pyruvate dehydrogenase complex of ascaris muscle. | pyruvate dehydrogenase complex and lipoamide dehydrogenase were purified from muscle of ascaris lumbricoides var. suum which contains relatively a large amount of the complex. molecular weights of three constituent enzymes of ascaris pyruvate dehydrogenase complex were as follows; alpha- and beta-subunits of pyruvate dehydrogenase were 42,000 and 37,000, respectively, lipoate acetyltransferase was 76,000 and lipoamide dehydrogenase was 56,000. furthermore, two unknown polypeptides having molecul ... | 1986 | 3099796 |
| [intestinal parasitic colonization and protein-caloric malnutrition]. | intestinal parasitic infection was determined by fecal examination for parasites with several complementary methods in 380 young gabonese infants aged 6 to 24 months (190 with normal weights and 190 with malnutrition, matched for age). trichuris trichiura, ascaris lombricoides, giardia intestinalis, entamoeba histolytica and strongyloides stercoralis were the most prevalent parasites, without any difference between well-fed infants and patients with malnutrition. the higher prevalence of strongy ... | 1986 | 3101646 |
| quaternary structure of erythrocruorin from the nematode ascaris suum. evidence for unsaturated haem-binding sites. | the quaternary structure of erythrocruorin from the nematode ascaris suum was studied. the native protein had a sedimentation coefficient, at a protein concentration of 1 mg/ml, of 11.6 +/- 0.3 s and an mr, as determined by sedimentation equilibrium, of 332,000 +/- 17,000. sds/polyacrylamide-gel electrophoresis gave one band with a mobility corresponding to an mr of 43,000 +/- 2000. the mr of the polypeptide chain was determined to be 41,600 +/- 1,500 by sedimentation equilibrium in 6 m-guanidin ... | 1987 | 3109399 |
| biochemical and immunological systematics of some ascaridoid nematodes: genetic divergence between congeners. | vertical starch gel electrophoresis and trefoil immunodiffusion were used to study the systematics of some ascaridoid nematodes. within the ascarididae, the time scale of divergence was too great for intergeneric electrophoretic comparisons. congeneric electrophoretic comparisons of baylisascaris procyonis (host--raccoon) versus baylisascaris transfuga (host--black bear), and toxocara canis (host--domestic dog) versus toxocara cati (host--domestic cat) yielded nei genetic distance coefficients o ... | 1987 | 3114467 |
| strain difference in an allergic asthma model in rats. | a new rat asthma model was devised, and with the model, allergic bronchoconstrictor responses and effects of disodium cromoglycate (dscg) were compared among wistar, lewis and fischer 344 rats. rats were actively sensitized with dnp-ascaris antigen (dnp-as) and killed bordetella pertussis vaccine. after eight days, asthmatic response was provoked by inhalation of dnp-as. the bronchomotor response was measured with a modified konzett-rössler method in diaphragm-sectioned rats. the inhalation of d ... | 1987 | 3119903 |
| a major role for phospholipase a2 in antigen-induced arachidonic acid release in rat mast cells. | cross-linking of ige receptors by antigen stimulation leads to histamine release and arachidonic acid release in rat peritoneal mast cells. investigators have reported a diverse distribution of [3h]arachidonate that is dependent on labelling conditions. mast cells from rat peritoneal cavity were labelled with [3h]arachidonic acid for different periods of time at either 30 or 37 degrees c. optimum labelling was found to be after 4 h incubation with [3h]arachidonate at 30 degrees c, as judged by c ... | 1987 | 3120703 |
| comparative study of amino acid contents and ldh isozyme by electrophoresis and slab-page in adult worms of ascaris lumbricoides, ascaris suum and toxocara canis. | | 1987 | 3127150 |
| human antibody responses against schistosomal antigens. i. antibodies from patients with ancylostoma, ascaris lumbricoides or schistosoma mansoni infections react with schistosome antigens. | sera of patients with either hookworm infections or ascariasis, who are from areas nonendemic for schistosoma mansoni, contain antibody reactivity against extracts of s. mansoni schistosomula. such patients with hookworm, but not those with ascaris lumbricoides, also express considerable antibody activity against intact schistosomula and schistosomular membrane preparations. sera from patients with schistosomiasis mansoni also contain these activities. because these three helminthic infections o ... | 1988 | 3128128 |
| role of cyclooxygenase products of arachidonic acid metabolism in ascaris antigen-induced bronchoconstriction in sensitized dogs. | the pharmacologic modulation of ascaris allergen-induced bronchoconstriction was investigated in beagle dogs sensitized by experimental infection with ascaris suum ova. ascaris-induced increases in total lung resistance (rl) and falls in dynamic lung compliance (cdyn) were unaffected by pretreatment with an antihistamine (pyrilamine) given alone but were significantly attenuated (p less than .05) by the cyclooxygenase enzyme inhibitor, indomethacin. the combination of pyrilamine and indomethacin ... | 1988 | 3130478 |
| [glycogen synthetase in helminths. inhibition by benzimidazoles]. | | 1987 | 3130654 |
| [the immunostimulatory effect of nonspecific transfer factor on t- and b-dependent lymphocytes in guinea pigs during its protective action in ascariasis]. | | 1988 | 3130712 |
| modification of bronchial blood flow during allergic airway responses. | ascaris suum antigen effects on mean airflow resistance (rl) and bronchial arterial blood flow (qbr) were studied in allergic anesthetized sheep with documented airway responses. qbr was measured with electromagnetic flow probes, and supplemental o2 prevented antigen-induced hypoxemia. aerosol challenge with this specific antigen increased rl and qbr significantly. cromolyn sodium aerosol pretreatment prevented antigen-induced increases in rl but not in qbr. intravenous cromolyn, however, preven ... | 1988 | 3136132 |
| late-phase reaction in topically induced ocular anaphylaxis in the rat. | a cellular late-phase reaction is described in a rat model of topically induced ocular anaphylaxis. rats were immunized with dinitrophenylated ascaris suum extract and alum and were tested for active cutaneous anaphylaxis on day 13. rats with a strong skin test response were selected for ocular challenge with di-dnp-lysine. macroscopic observation and histologic evaluation were performed at 1, 6, and 24 h. in rats showing a moderate macroscopic ocular response at 1 h, mast cell degranulation was ... | 1988 | 3136973 |
| effect of strongyloides ratti on small bowel function in normal and immunosuppressed host rats. | although strongyloides stercoralis is a common parasite, little is known about its effect on intestinal function. published clinical studies are difficult to evaluate and compare because of the inability to differentiate the effects of the parasite load from that of various other coexisting features such as bacterial overgrowth, multiparasitism, malnutrition, or tropical sprue. using a rat model where these problems do not occur, we found that strongyloides ratti did not inhibit intestinal funct ... | 1988 | 3139382 |
| attenuation of rat conjunctival response by repeated hapten applications. | attenuation of the rat conjunctival response by repeated topical challenge with dinitrophenyl (dnp) hapten was demonstrated in our study. adult rats were immunized by intraperitoneal injections of dinitrophenylated ascaris suum extract (dnp-asc) and alum. serum levels of anti-dnp homocytotropic antibody were determined by passive cutaneous anaphylaxis in rats prepared with antibody 48 hours earlier. in other animals, topical challenge was performed by applying n,n'-di-2,4-dnp-l-lysine (di-dnp-ly ... | 1988 | 3141114 |
| chalone-like inhibition of dna synthesis in helminths. | chalone-like inhibitory activity was found in extracts from tissues of taenia crassiceps cysticercus and tissues of adult ascaris suum. the inhibitory effects were partially species nonspecific: the extracts from t. crassiceps larvae inhibit both t. crassiceps and mesocestoides corti larvae dna synthesis. extracts from a. suum, however, inhibit only t. crassiceps. besides them, the inhibitory factor from mouse brain inhibits both t. crassiceps and m. corti dna synthesis. when the inhibitory fact ... | 1985 | 3159635 |
| endoscopic retrograde cholangiopancreaticographic features of pancreaticobiliary ascariasis. | endoscopic retrograde cholangiopancreaticographic features of ascariasis involving the biliary system (56) and pancreatic duct (4) in 60 consecutive patients have been analyzed and their various radiologic appearances described. ascaris not only presents as a smooth linear filling defect, as commonly described in the literature, but also in a variety of other shapes like parallel smooth filling defects, curves, and loops crossing the hepatic ducts transversely. they can also cause dilatation of ... | 1988 | 3169480 |
| prostaglandins in ascaris antigen. | | 1988 | 3170997 |
| small intestinal perforation by ascaris lumbricoides. | | 1988 | 3176145 |
| a survey of gastrointestinal parasites in ogun state, southwest nigeria. | to study the epidemiology of intestinal parasites in ogun state, nigeria, 479 stool specimens were examined at a hospital in abeokuta during the rainy season in june 1986. intestinal helminths and/or protozoa were present in 297 samples (62%). 41.1% of the specimens showed a single infection, 34% showed double, 20% triple and 5% quadruple infection. the most commonly found worm was ascaris lumbricoides (in 40% of specimens), followed by trichuris trichiura (23.2%), hookworms (19.2%), strongyloid ... | 1988 | 3178337 |
| clustering of ascaris lumbricoides and trichuris trichiura infections within households. | a survey of 428 households in a shanty town in coatzacoalcos, mexico, revealed high prevalences of ascaris lumbricoides and trichuris trichiura. the data were analysed separately for a. lumbricoides and t. trichiura in order to investigate the spatial distribution of heavy infections through the town. within each age class, those individuals with egg counts in the upper 20% of the range for that age were classified as "heavily infected". when the data were stratified by household size, it was fo ... | 1988 | 3188157 |
| age-related prevalence, intensity and frequency distribution of gastrointestinal helminth infection in urban slum children from kuala lumpur, malaysia. | the gastrointestinal helminth infection status of 1574 children living in a slum area of kuala lumpur, malaysia was assessed by quantitative coprology. almost two-thirds were infected with trichuris trichiura, 49.6% with ascaris lumbricoides, and 5.3% with hookworm. infection prevalence rose rapidly to a stable asymptote at 7 years of age, and the age-intensity profile was convex with maximal values in the 5-10 year age classes. this pattern was the same for males and females, but differed marke ... | 1988 | 3188158 |
| an observation of the dynamics of intestinal helminth infections in two isolated communities in south-western nigeria. | school pupils aged 8-12 years with triple infections of ascaris lumbricoides, trichuris trichiura and hookworms at iwaya, a large urban slum in lagos state and iworoko/igbemo, a rural farming community in ondo state, nigeria, were given single dose treatment of levamisole (40 mg.). subsequent worm accumulation was studied by egg counts per gram of faeces on the 2nd week, 3rd, 5th, 9th and 12th months after treatment. worm infection rates and load were heavier at iwaya. the rates of worm recruitm ... | 1988 | 3188213 |
| activity of a novel hydroquinone inhibitor of leukotriene synthesis (u-66,858) in the rhesus monkey ascaris reactor. | the ige-mediated hypersensitivity to ascaris antigen in reactor rhesus primates was used to assess the pharmacologic profile of u-66,858 (1-acetoxy-2-n-butyl-4-methoxy-naphthalene). when the compound was given by the oral route, it showed dose-related inhibition of resistance (rl) and compliance (cdyn) changes. when the compound was given by the aerosol route, it showed dose independent inhibition. in 15 animals, aerosols (52 +/- 32 to 53 +/- 10% for rl, p = 0.05 and 45 +/- 19 to 28 +/- 19% cdyn ... | 1988 | 3192307 |
| efficacy of flubendazole against gastrointestinal and lung nematodes in pigs. | three trials were carried out on landrace pigs of various ages to assess the anthelmintic efficacy of flubendazole. the pigs were either artificially infected with metastrongylus apri or naturally or artificially infected with the gastrointestinal nematodes ascaris suum, oesophagostomum dentatum or hyostrongylus rubidus. for mass medication of young pigs and fatteners a dose regimen of 30 ppm flubendazole in the feed for 10 consecutive days was 100 per cent effective against the four nematode sp ... | 1988 | 3195017 |
| ivermectin in loiasis and concomitant o. volvulus and m. perstans infections. | two clinical studies were carried out in gabon, africa to evaluate the efficacy, safety, and tolerability of ivermectin in the treatment of patients with loa loa infection. in the first study, 35 patients received single oral doses of ivermectin, 5-200 mcg/kg body weight. blood microfilariae levels did not decrease after a single oral 5, 10, 30, or 50 mcg/kg dose of ivermectin, but levels did decrease after doses of 100, 150, and 200 mcg/kg. the most efficacious dose was 200 mcg/kg; mean blood m ... | 1988 | 3195695 |
| phosphorylation and inactivation of the pyruvate dehydrogenase from the anaerobic parasitic nematode, ascaris suum. stoichiometry and amino acid sequence around the phosphorylation sites. | tryptic digestion of the fully phosphorylated ascaris suum pyruvate dehydrogenase complex yielded a single tetradecapeptide containing 2 phosphorylated serine residues. its amino acid sequence was tyr-ser-gly-his-ser(p)-met-ser-asp-pro-gly-thr-ser(p)-tyr-arg and was very similar to one of the tryptic phosphopeptides isolated from mammalian and yeast pyruvate dehydrogenases. at partial phosphorylation, three peptides were isolated which corresponded to the monophosphorylated (sites 1 and 2) and d ... | 1988 | 3198613 |
| superoxide dismutase from ascaris suum. | three superoxide dismutases (sod) (ec 1.15.1.1) were detected in homogenates of ascaris suum. each of the three forms of sod was purified by a sequence of multiple differential centrifugations, ammonium sulphate precipitation, ion-exchange chromatography and g-75 sephadex column chromatography. the three forms of sod were present in different cellular locations; one in the cytoplasmic fraction, sensitive to cyanide and hydrogen peroxide, and two in the mitochondrial fraction, one of which was cy ... | 1988 | 3200607 |
| cellular markers of inflammation in the airways of allergic sheep with and without allergen-induced late responses. | allergic sheep respond to inhaled ascaris suum antigen either with an acute bronchoconstriction alone (acute responders, ar) or both an acute and late bronchoconstriction (dual responders, dr). in this study, we determined if: (1) inflammatory cell composition of bronchoalveolar lavage (bal) obtained during the late response differs between dr and ar; (2) the difference in inflammatory cells is dependent on the prechallenge bal cell composition; and (3) drugs that block late airway responses als ... | 1988 | 3202508 |
| purification and properties of cathepsin d from rat yoshida ascites hepatoma ah-130. | cathepsin d was affinity-purified on pepstatin-sepharose from control rat liver, from yoshida ascites hepatoma (ah-130) cells, and from the liver of ah-130 tumour-bearing rats. apparent molecular mass and immunological reactivity, as determined by sds-page and immunoblotting, were identical for the three enzyme preparations. the active enzyme concentrations were determined by active-site titration. catalytic parameters were measured for the three enzymes using two synthetic chromogenic peptides ... | 1988 | 3202970 |
| a leukotriene and thromboxane inhibitor (sch 37224) blocks antigen-induced immediate and late responses and airway hyperresponsiveness in allergic sheep. | peptide leukotrienes and thromboxane a2 are putative mediators of allergen-induced late responses and allergen-induced airway hyperresponsiveness (ahr), respectively. sch 37224 blocks antigen-induced leukotriene d4 and thromboxane b2 release in guinea pig lung fragments. it also inhibits leukotriene-mediated allergic guinea pig bronchospasm. sch 37224 was, therefore, tested in allergic sheep (n = 6) with documented immediate and late airway responses and ahr to inhaled ascaris suum antigen. for ... | 1988 | 3204511 |
| a related moderately repetitive dna family in the nematodes ascaris lumbricoides and panagrellus silusiae. | digestion of genomic dna from the nematodes panagrellus silusiae and ascaris lumbricoides with restriction endonuclease bamh1 releases a 0.7 kilobase (kb) fragment. the 0.7 kb fragment from both nematodes was cloned onto e. coli plasmid puc19. using representative clones as dna hybridization probes, it was found that (i) the bamh1 fragments cross-hybridize; (ii) a ladder-effect with multiples of 0.7 kb was evident in both species after hybridization to genomic dna and (iii) the genomic copy numb ... | 1988 | 3205722 |
| isolation and purification of malate dehydrogenase in helminth parasites. | | 1988 | 3218244 |
| [methods of cultivating parasitic nematoda in vitro]. | | 1988 | 3218253 |
| [effects of traxanox sodium on experimental nasal allergy]. | the anti-allergic actions of traxanox sodium pentahydrate (traxanox) were examined using experimental allergic rhinitis models. traxanox (10 and 30 mg/kg, p.o.), tranilast (30 and 100 mg/kg, p.o.) and disodium cromoglycate (dscg, 3 and 10 mg/kg, i.v.) dose-dependently inhibited the increase of dye leakage in rats actively sensitized with dinitrophenol-coupled ascaris extract. traxanox was about 4 times more potent than tranilast. in this test, the inhibitory activity of traxanox (30 mg/kg, p.o.) ... | 1988 | 3220322 |
| extension of round worm through ear. | | 1988 | 3220522 |
| the induction of permeability in egg-shells of ascaris suum prior to hatching. | | 1988 | 3225120 |
| suppression of ige antibody production by ascaris suum extract: characterization of suppressive component(s). | partial characterization of the suppressive component(s) of a. suum extract that is (are) responsible for damping production of ige antibody to ovalbumin was performed by physical and chemical methods. digestion of the whole extract with trypsin and chymotrypsin completely abolished the suppressive activity. oxidation with sodium metaperiodate or heating at 56 degrees c, however, had no effect. these results indicate that the integrity of heat-stable protein(s) present in the crude extract is es ... | 1988 | 3228633 |
| airway obstruction due to ascaris lumbricoides. | | 1988 | 3229820 |
| nasogastric tube obstruction by ascaris lumbricoides. | | 1988 | 3229825 |
| reinfection with ascaris lumbricoides after chemotherapy: a comparative study in three villages with varying sanitation. | this study examined the effect of the 2 major means of control of ascaris lumbricoides--chemotherapy and sanitation. about 200 pre-school caribbean children living in 3 villages with varying sanitation were studied by quantitative stool examination for the presence of the eggs of helminths. children with ascaris eggs were treated with piperazine. over a 2-year period this procedure was repeated after every 6 months of natural re-infection. results showed that (i) the rate of reinfection was on a ... | 1988 | 3232186 |
| zoonotic parasite infections of the arabian sacred baboon papio hamadryas arabicus thomas in asir province, saudi arabia. | evidence of zoonotic parasite infection was recorded in the faeces of the arabian sacred baboon in human populated residential and non-residential areas in asir, southwestern saudi arabia. cysts of giardia intestinalis and entamoeba histolytica, and ova of hymenolepis nana, enterobius vermicularis, ascaris sp., trichuris sp., and a hookworm were encountered in 45 (about 39%) of 115 faecal samples examined. a higher rate of infection was observed in the residential areas. most of the parasites re ... | 1988 | 3232917 |
| intestinal complications of round worms in children. | one hundred forty-two patients with surgical complications of ascaris lumbricoides were treated in our hospital over a period of 5 years. included were 120 patients with subacute intestinal obstruction that were treated conservatively, and 22 patients with acute intestinal obstruction that required surgical intervention. four of the 22 patients that were operated on died following various postoperative complications. however, there were no deaths among those presenting with subacute obstruction. ... | 1988 | 3236163 |
| immunocytochemical localization of actin in toxoplasma gondii. | the localization of actin in the trophozoites of toxoplasma gondii was examined by means of immunogold staining for electron microscopy. the thin-sectioned specimens were incubated with the igg fraction of polyclonal antibodies raised against ascaris body-wall smooth-muscle actin following colloidal gold-conjugated protein a. electron-dense gold particles were confined to the anterior polar region in the trophozoites: they were found in the conoid, preconoidal rings, possibly in the polar ring, ... | 1988 | 3237674 |
| [ascaris infestation: a possible explanation for the high levels of anti-a igg observed in the venezuelan population]. | | 1988 | 3239344 |
| conservative management of intestinal obstruction due to ascaris worms in adult patients: a preliminary report. | | 1988 | 3244137 |
| parasitism and nutritional status of poor suburban jamaican toddlers. | a survey was conducted on 145 toddlers, 6-36 months of age, in a suburb in kingston, jamaica. the aim of the study was to measure the prevalence of and association between parasitism and nutritional status. 42% were malnourished (80% of standard weight for age), most commonly (76%) in the 12-17 month age group. 34% had 1 or more parasites, particularly trichuris trichuria (21%) or ascaris lumbricoides (63%). peak prevalence of parasitism was among the 30-36 month old children (63%). nutriti ... | 1988 | 3247726 |
| allergen challenge induces vasodilatation in pig bronchial circulation via a capsaicin-sensitive mechanism. | | 1988 | 3250224 |
| [inhibitory effects of extracts of ascaris suum on human blood coagulation]. | | 1988 | 3253027 |