| antiamoebic activity of chonemorphine, a steroidal alkaloid, in experimental models. | chonemorphine, a steroidal alkaloid isolated from chonemorpha fragrans moon (apocyanaceae) was identified as an antimoebic principle during the course of a screening programme for novel antiparasitic agents from plant sources. at a dosage of 100 mg/kg x 4 chonemorphine led to a 100% cure of experimental hepatic infection in golden hamsters and cleared 90% of the intestinal infection in weanling wistar rats at 200 mg/kg (x4) dosages. the discovery of chonemorphine as an antiamoebic agent is an ad ... | 1987 | 2894025 |
| free fatty acids released from phospholipids are the major heat-stable hemolytic factor of entamoeba histolytica trophozoites. | the major hemolytic activity of entamoeba histolytica trophozoites is located in a vesicular fraction called p30 and known to be due to heat-labile and heat-stable hemolytic components whose effect increases up to 100 times during preincubation at 36 degrees c. the heat-stable hemolytic activity (hsha) was found in the chloroform-methanol extract of preincubated p30, whose partition with 2 m kcl yielded a lipid fraction, an interphase, and an aqueous phase. hsha was detected only in the lipid fr ... | 1988 | 2894362 |
| increase in activity and biosynthesis of phospholipase a of entamoeba histolytica by cholesterol passage. | | 1987 | 2894368 |
| immunological differentiation of pathogenic and non-pathogenic isolates of entamoeba histolytica. | entamoeba histolytica can act as a harmless commensal organism in the lumen of the large intestine, or can cause invasive amoebiasis. some workers have suggested that there are two distinct subspecies of this organism, and that only one of these is associated with invasive disease. present isoenzyme tests to identify the subspecies take several days to analyse: we report a technique that uses immunofluorescence with monoclonal antibodies, takes two days to perform, and may, therefore, assist in ... | 1988 | 2894495 |
| isoenzyme patterns of entamoeba histolytica isolated from homosexual men. | this is a preliminary report on isoenzyme patterns of entamoeba histolytica which were examined by starch gel electrophoresis. by this means the amoebae isolated from 17 asymptomatic male homosexuals were characterized into zymodemes. no amoebic strain isolated corresponded to a pathogenic zymodeme. | 1987 | 2894714 |
| immunological characterization of axenic entamoeba histolytica related antigens. | | 1987 | 2895066 |
| plant based antiamoebic drugs; part i. antiamoebic activity of phenanthroindolizidine alkaloids; common structural determinants of activity with emetine. | | 1987 | 2895482 |
| entamoeba histolytica in inbred mice: evidence of liver involvement. | following observations that certain mouse strains appeared to be susceptible to liver invasion by entamoeba histolytica, mouse strains cba/hn, cba/hn, nu/nu, cba/hn nu/+, cba/hn dh/+, cba/hn +/+, c3h/hej lps/lps and ab/h, were infected intra-intestinally with strain saw 408 (zymodeme ii) of the amoeba. a number of mice from each group died during the first 14 days with acute lesions. the remainder were killed at 4 and 6 months after infection. cba/hn, cba/hn dh/+, and cba/hn +/+ mice all had amo ... | 1987 | 2895511 |
| the effect of orally administered entamoeba histolytica on wistar and athymic (rnu rnu) rats observed during a 12-month period. | groups of lacp (wistar) and rnu rnu athymic rats were dosed orally with cultured trophozoites of 2 strains of entamoeba histolytica, zymodeme ii, and observed for up to 12 months. one group of wistar rats remained infected but with no apparent disease for 6 months; faecal samples from another became negative after one week. all the athymic rats were still infected at 12 months and amoebae could be cultured from their stools. the effect of even long-term infection was usually only mild erosion of ... | 1987 | 2895512 |
| immunocytochemical detection of entamoeba histolytica. | human anti-entamoeba histolytica immunoglobulin was used to detect entamoeba histolytica in 74 positive samples from several different sources, using an indirect immunoperoxidase method. in 73 samples, the protozoan was easily identified. trophozoites and cysts of all cultured entamoeba strains examined were strongly stained, and as few as 3 trophozoites per microscope slide could be detected. in addition, 51 negative control samples were also tested and non-specific reactions were not observed. ... | 1987 | 2895513 |
| an enzyme-linked immunosorbent assay for the detection of entamoeba histolytica antigens in faecal material. | this paper describes a method for the detection of entamoeba histolytica antigens in stool samples using a multi-layer elisa. the method is sensitive and specific, showing no interference with other intestinal parasites, e.g. e. coli, e. hartmanni, endolimax nana, iodamoeba buetschlii, hymenolepis nana, giardia lamblia, trichomonas and ascaris. the method provides a rapid and simple screening assay for e. histolytica infections and should assist in diagnosis and epidemiological studies of the di ... | 1987 | 2895514 |
| epidemiologic and clinical features of invasive amebiasis in bangladesh: a case-control comparison with other diarrheal diseases and postmortem findings. | to describe the epidemiologic and clinical features associated with invasive amebiasis in bangladesh, 85 hospitalized diarrheal patients with hematophagous trophozoites of entamoeba histolytica in their stools were compared to a control group of 84 hospitalized diarrheal patients without amebiasis. postmortem examinations were carried out in 22 deaths due to amebiasis. for the patients with amebiasis, there was a bimodal age distribution with peaks at 2-3 years and greater than 40 years, whereas ... | 1988 | 2895590 |
| acid phosphomonoesterase heterogeneity in axenically grown entamoeba histolytica. | | 1987 | 2895609 |
| [the importance of entamoeba histolytica in persons returning from the tropics]. | stools from 1000 travellers returning from the tropics were examined for intestinal parasites. the most frequently isolated intestinal pathogens were giardia lamblia (5%) and e. histolytica (3.6%). for 21 out of 36 e. histolytica isolates iso-enzyme differentiation by starch-gel electrophoresis was possible, yielding a pathogenic pattern in four. this finding correlated closely with clinical symptoms and positive serology, while e. histolytica with non-pathogenic zymodeme should not be considere ... | 1988 | 2896113 |
| amoebic hepatitis as a precursor of suppurative amoebic liver abscess. | | 1988 | 2896166 |
| travelers' diarrhea in west africa and mexico: fecal transport systems and liquid bismuth subsalicylate for self-therapy. | the goals of this study were threefold: to compare the etiology of travelers' diarrhea in west africa and mexico, to evaluate two fecal transport systems for the recovery of enteropathogens, and to verify the efficacy of liquid bismuth subsalicylate (bss) in different locations and under different entrance criteria for disease severity. the study populations consisted of 133 european tourists in west africa and 112 american students in mexico who had suffered from travelers' diarrhea. in 60% and ... | 1988 | 2896219 |
| use of chinese hamster ovary cells with altered glycosylation patterns to define the carbohydrate specificity of entamoeba histolytica adhesion. | we compared the adherence of e. histolytica trophozoites with a panel of lectin-resistant cho mutants with altered glycosylation patterns. our results coupled with data from saccharide inhibition studies indicate that terminal n-acetyllactosamine units on asn-linked complex type oligosaccharides provide the major determinants on the cellular receptor for e. histolytica adhesion. | 1988 | 2896759 |
| pathogenic entamoeba. | | 1988 | 2896984 |
| amoebicidal activity of nitrovinylindole. | | 1987 | 2897332 |
| phorbol esters specifically enhance the cytolytic activity of entamoeba histolytica. | entamoeba histolytica causes invasive amebiasis by lysis of host tissue and inflammatory cells. the in vitro cytolysis of target chinese hamster ovary (cho) cells by axenic e. histolytica trophozoites (strain hm1:imss) is a calcium- and phospholipase a-dependent event initiated by the binding to the target cell of the galactose-inhibitable surface lectin of the parasite. we utilized phorbol esters as a probe to determine whether an amebic protein kinase c has a role in the cytolytic event. the a ... | 1988 | 2897334 |
| relationship of free intracellular calcium to the cytolytic activity of entamoeba histolytica. | entamoeba histolytica adherence and destruction of host cells is required for in vivo pathogenicity; amebic in vitro adherence is mediated by a galactose- or n-acetyl-d-galactosamine-inhibitable surface lectin (gal/galnac adherence lectin). free intracellular ca2+ concentration [( ca2+]i) was measured in living amebae and target cells during amebic cytolysis of chinese hamster ovary (cho) cells and human polymorphonuclear neutrophils by utilizing the ca2+ probe fura-2 and computer-enhanced digit ... | 1988 | 2897335 |
| interaction between pathogenic amebas and fibronectin: substrate degradation and changes in cytoskeleton organization. | invasion of human tissues by the parasitic protozoan entamoeba histolytica is a multistep process involving, as a first step, the recognition of surface molecules on target tissues by the amebas or trophozoites. this initial contact is followed by the release of proteolytic and other activities that lyse target cells and degrade the extracellular matrix. in other parasitic diseases, as well as in certain cancers, the interaction of invasive organisms or cells with fibronectin (fn) through specif ... | 1988 | 2897372 |
| the immune response in amoebiasis. | | 1988 | 2897401 |
| amebiasis. the ancient scourge is still with us. | amebiasis is usually contracted in geographic areas where sanitation is poor, but outbreaks can still occur anywhere that drinking water becomes contaminated with sewage. in the majority of persons infected with the parasite, colonization of the intestine is asymptomatic. in others, symptoms of gastrointestinal distress can appear within a week. in rare cases, extra-intestinal amebiasis can cause abscesses in the liver or elsewhere. many questions about the disease course in different patients r ... | 1988 | 2897682 |
| [colitis--recent trends and countermeasures. mycoses, protozoan and parasitic diseases]. | | 1988 | 2898028 |
| factors influencing the pathogenicity of entamoeba histolytica. | | 1988 | 2898182 |
| amoebic pericardial effusion. | | 1988 | 2898540 |
| [possible entry of fragments of a bacterial genome into the trophozoite nucleus of the dysentery amoeba]. | the use of fölgen-like electron microscopy reaction has revealed particles in the cytoplasm and nucleus of the dysentery amoeba trophozoites which in their size and resistance to acidic hydrolysis and in their affinity for uranilacetate are similar to tightly packed elements of the bacterial genome. on this basis a conclusion was drawn that some fragments of genomes of phagocytic bacteria can be preserved in the amoebal cell. the community of microorganisms consisting of trophozoites of the dyse ... | 1988 | 2898747 |
| affinity purification and biochemical characterization of histolysin, the major cysteine proteinase of entamoeba histolytica. | we report a one-step method for the purification to homogeneity of a cysteine proteinase of entamoeba histolytica (histolysin) by affinity chromatography of the soluble extract of the parasite on immobilized phenylalanyl(2-phenyl)aminoacetaldehyde semicarbazone. the enzyme has an apparent mr of 26,000 by sds/polyacrylamide-gel electrophoresis and 29,000 by gel chromatography. its ph optimum varies widely, from 5.5 with azocasein to approx. 7 with other protein substrates and benzyloxycarbonylphe ... | 1988 | 2898937 |
| in vitro killing of entamoeba histolytica trophozoites by interferon-gamma-activated mouse macrophages. | the effect of murine interferon gamma (ifn-gamma) on macrophage activation for amoebicidal activity was examined. peritoneal macrophages were harvested from c57bl/6 mice and preincubated with ifn-gamma and/or lipopolysaccharide (lps). in vitro amoebicidal activity of these macrophages was determined by trypan blue exclusion test against a virulent strain of e. histolytica (ip:0682:1). it was found that in vitro amoebicidal activity was evident in macrophage monolayers treated with both ifn-gamma ... | 1988 | 2899056 |
| [interaction of entamoeba histolytica and antibodies in situ]. | | 1987 | 2899424 |
| entamoeba histolytica: virulence potential and sensitivity to metronidazole and emetine of four isolates possessing nonpathogenic zymodemes. | the pathogenic potential of four entamoeba histolytica isolates obtained from asymptomatic carriers and possessing nonpathogenic zymodemes was compared to four e. histolytica strains obtained from invasive cases of amebiasis and having pathogenic zymodemes. both xenic and axenic cultures of a number of strains were tested. determinations of cytopathogenicity were done in vitro by measuring the rates of destruction of tissue cultured monolayers of baby hamster kidney cells by intact amebae or by ... | 1988 | 2899517 |
| entamoeba histolytica: potent in vitro antiamoebic effect of gossypol. | | 1988 | 2899518 |
| freeze-fracture study of the trophozoite and the cyst of entamoeba histolytica. | the fine structure of the trophozoite and cyst of entamoeba histolytica from the stool of a patient was compared using the freeze-fracture method. the intramembranous particles (imp's) were heterogeneously distributed on the plasma membrane of the trophozoite and their density was 1139 +/- 105/micron 2 on the p face. particle-rich depressions and linear particle arrays, reported by other investigators on cultured trophozoites, were also observed on the p face while on the e face such special par ... | 1988 | 2899642 |
| susceptibility of hamsters to caecal amoebiasis by oral infection. | a method is described for successfully establishing caecal amoebiasis in hamsters which were not fed and which were pretreated with 1 ml of magnesium sulphate every 24 hours for three days and then given 12 x 10(5) trophozoites of the hm-1 axenic strain or 18 x 10(5) trophozoites of the hk-9 axenic strain of entamoeba histolytica by the oral route. all the animals developed diarrhoea within 24 hours of infection. when the animals were killed on the fifth day after infection the caecum was swolle ... | 1988 | 2899994 |
| entamoeba histolytica antigens as possible vaccinogens? a short review. | a few key papers which have recently been published on the characterization of amoeba antigens are reviewed. immunofluorescence tests and immunoelectron microscopy have demonstrated the localization of certain surface antigens on axenically cultured trophozoites. most of the surface antigens have largely been shown to elicit a humoral response. the elicitation of cellular response has not been well illustrated. the localization of a large number of antigens in cytoplasmic vacuoles and plasma mem ... | 1988 | 2900249 |
| binding and internalization of rat colonic mucins by the galactose/n-acetyl-d-galactosamine adherence lectin of entamoeba histolytica. | purified rat colonic mucins inhibit entamoeba histolytica in vitro adherence to and lysis of colonic epithelial cells by binding to the amoebic galactose/n-acetyl-d-galactosamine (gal/galnac)-inhibitable adherence lectin. we found that 125i-labeled mucins demonstrated saturable gal-specific binding to e. histolytica trophozoites (strain hm1:imss), with 2.8 x 10(3) binding sites per amoeba and a dissociation constant of 8.20 x 10(-11) m-1. inhibition of parasite protein synthesis completely abrog ... | 1988 | 2900266 |
| efficacy of a single dose of secnidazole in the treatment of acute and chronic amoebiasis. | an open study was carried out to determine the clinical efficacy of secnidazole in the treatment of acute and chronic cases of amoebiasis. patients were given 2 g of secnidazole in a single dose or in two divided doses within a 4 h interval. details were recorded daily by patients of complaints such as fever, lumbar pain, lbm, dizziness, body weakness, bloody mucoid stools, epigastric pains and other symptoms, before and after treatment. results were analysed for 45 chronic and five acute cases ... | 1988 | 2900339 |
| observations on the interpretation of amoebic serology in endemic areas. | antibody to entamoeba histolytica antigen was evaluated in cases of proven and suspected intestinal amoebiasis as well as in extra-intestinal cases from an endemic area. screening methods included the gel diffusion precipitation test (gd), counter immunoelectrophoresis (cie) and the indirect haemagglutination test (iha). control populations consisting of asymptomatic cyst passers, non-amoebic individuals, patients with giardiasis and cases of enteric fever were also screened using the above test ... | 1988 | 2900340 |
| plant based antiamoebic drugs; part ii. amoebicidal activity of parthenin isolated from parthenium hysterophorus. | | 1988 | 2900531 |
| [histochemical studies of lipid metabolism in entamoeba histolytica (schaudinn, 1903)]. | | 1987 | 2900579 |
| [the morphology of amebiasis]. | amebiasis--a protozoan human disease caused by entamoeba histolytica--occurs in the ussr sporadically in particular geographic regions and in particular populations. the paper reports morphologic findings made in patients with amebiasis involving the intestine or other organs. in typical cases, amebiasis affects the large intestine, particularly the cecum and ascending colon, and has the development of distinctive ulcers as its characteristic feature. as the disease progresses, these appear in a ... | 1988 | 2900628 |
| entamoeba histolytica antigen-specific induction of human immunodeficiency virus replication. | replication of human immunodeficiency virus (hiv) may be initiated in infected lymphocytes by antigenic or mitogenic stimulation. a soluble protein derived from an invasive strain of entamoeba histolytica (amoebic antigen [aa]) was used to study the lymphoblastic responses of t lymphocytes derived from 8 hiv-seronegative homosexual men (controls) and 15 hiv-seropositive homosexual men (patients). the soluble protein was also used in long-term cultures as a stimulus for hiv replication. no contro ... | 1988 | 2900844 |
| trophozoite and nuclear size, dna base composition, and nucleotide sequence homology of several entamoeba strains in axenic culture. | we carried out a comparative study of nuclear and trophozoite diameters and of dna thermal denaturation in eight entamoeba strains cultured axenically (four of them e. histolytica, two initially designated as e. invadens, one e. moshkovskii, and one e. histolytica-like), as well as an analysis of the overall dna sequence homology of the non-e. histolytica strains. the average nuclear (n) and trophozoite (t) diameters (in micron) were, respectively: global averages +/- sd for e. histolytica strai ... | 1988 | 2901079 |
| [a modified tanabe-chiba medium for detection of entamoeba histolytica]. | tanabe-chiba's (t-c) medium, which has been routinely used for detection of entamoeba histolytica in feces in japan, was modified by adding some commercially available enrichments to the liquid part and by eliminating the agar slant. this is tentatively called m medium. m medium and t-c medium were compared for their efficiency for detecting e. histolytica in the feces of monkeys. no significant difference in the detection rate was found between the two media. however the rate of multiplication ... | 1988 | 2901365 |
| evaluation of enzyme-linked immunosorbent assay (elisa), indirect hemagglutination (iha), counterimmunoelectrophoresis (ciep), and immunodiffusion (id) in the serodiagnosis of amebiasis. | | 1988 | 2901446 |
| isolated amebic appendicitis: a pathologic rarity. | | 1988 | 2901812 |
| entamoeba histolytica: cell cycle and nuclear division. | the cell cycle of entamoeba histolytica, the duration of its phases, and the details of the nuclear division stages are described in this paper. trophozoites from clone l-6, strain hm1:imss, were synchronized by colchicine. synchrony was observed immediately after treatment and cultures remained synchronous for at least three replicative cycles with synchrony indexes between 13 and 15 hr. the stages of nuclear division were studied by light and electron microscopy. four stages of the nuclear div ... | 1988 | 2901981 |
| low susceptibility of trophozoites of virulent entamoeba histolytica to cellular and antibody-dependent cellular cytotoxicity by guinea-pig effector cells. | cytotoxic potentialities of lymphocytes and macrophages were determined against trophozoites of virulent and avirulent sublines of entamoeba histolytica in vitro. guinea-pigs were immunized with antigens of both sublines to obtain stimulated effector cells and antiamoebic antibodies. trophozoites of an avirulent/attenuated subline of e. histolytica (nih200) were significantly more susceptible to killing by lymphocytes and macrophages through cellular and antibody-dependent cellular cytotoxic mec ... | 1987 | 2902704 |
| the prevalence of entamoeba histolytica in lactating women and in their infants in bangladesh. | entamoeba histolytica was studied in 33 lactating women and their infants in a periurban village in bangladesh. infant-mother pairs were followed for a period of 10-15 months: 67% of mothers excreted e. histolytica during the observation period, the majority for 3 months or more. only one mother was symptomatic, with a mild, non-dysenteric diarrhoea. 58% of mothers were seropositive, several of them continuously and with a high titre, indicating past invasive infection. 67% had detectable antibo ... | 1988 | 2902705 |
| [endoscopic diagnosis of a cecal ameboma successfully treated with antibiotic therapy]. | | 1987 | 2902725 |
| movement of entamoeba histolytica trophozoites in rat cecum and colon intact mucus blankets and harvested mucus gels. | entamoeba histolytica trophozoites were centrifuged into mucus gels harvested from in vivo loops of rat cecum and proximal colon. frank ameba movement was not detected in the colonic mucus, but attenuated motility was measured in the cecal mucus. the harvested rat cecal mucus had significantly lower apparent viscosity and neutral glycoprotein concentration values than the colonic mucus. a shape factor method was developed to assess the motility of amebae in mucus gels and intact mucus blankets. ... | 1988 | 2902807 |
| on the specificity of a cysteine proteinase from entamoeba histolytica. | a cysteine proteinase has been isolated from the virulent strain hm1:imss of entamoeba histolytica by two gel chromatography steps, ion exchange chromatography on deae-cellulose and affinity chromatography on organomercurial-sepharose. the purified proteinase was homogeneous, as judged by polyacrylamide gel electrophoresis in the presence and in the absence of sodium dodecyl sulphate, and was a monomeric protein with a relative molecular mass of mr 27000 +/- 2000 possessing an n-terminal alanine ... | 1988 | 2902852 |
| outcome of untreated infection with entamoeba histolytica in homosexual men with and without hiv antibody. | among homosexual men the prevalence of infection with entamoeba histolytica is high. to determine the clinical importance of this infection 55 homosexual men carrying the parasite were investigated in detail. no clinical, serological, or histological evidence of invasive amoebiasis was found in any of them. the patients were not treated and were followed up for 12 to 29 months (mean 21.6 months), during which period none developed symptoms that could be attributed to e histolytica. spontaneous l ... | 1988 | 2902889 |
| enzyme-linked immunosorbent assay in the detection of antigen in amoebic liver abscess. | | 1988 | 2903119 |
| in vitro and in vivo studies of macrophage functions in amebiasis. | experimental intrahepatic inoculation of the gerbil with entamoeba histolytica trophozoites was used as a model of liver amebiasis to study the cellular immune response elicited by the parasite. it was shown that abscess-derived macrophages (5 to 20 days old) were deficient in their capacity to develop a respiratory burst, to secrete and express membrane-bound interleukin-1-like activity, and to kill e. histolytica trophozoites as well as to respond to lymphokines in vitro. however, macrophages ... | 1988 | 2903124 |
| amoebic liver abscess in a bisexual man. | a bisexual man who had not travelled outside the u.k. had an amoebic liver abscess. although it is reported that entamoeba histolytica is usually of a non-pathogenic type in homosexual men in the u.k., it is important to diagnose invasive amoebiasis in this high-risk group so that appropriate treatment can be given. | 1988 | 2903199 |
| nucleotide sequence analysis of an entamoeba histolytica ferredoxin gene. | a cdna clone (subclone b) previously isolated from the human parasite entamoeba histolytica was characterized. dna sequence analysis of subclone b identified the dna as that encoding apoferredoxin. e. histolytica ferredoxin cdna contains unusually short 5' and 3' noncoding regions of 9 and 25 nucleotides, respectively. a genomic ferredoxin clone was isolated from e. histolytica dna, and comparison of genomic and cdna sequences revealed that the ferredoxin gene is unspliced. the deduced amino aci ... | 1988 | 2903444 |
| pehps medium: an alternative for axenic cultivation of entamoeba histolytica and e. invadens. | knowledge of entamoeba histolytica biology in the last 17 years has been acquired largely as a consequence of this parasite's axenic cultivation in tps-1 or tyi-s-33 media. unfortunately, there are often low yields in these media, due to variability of their main components, panmede and yeast extract. we describe a medium, pehps, of which the main components are extracts of ox liver, and ox and swine pancreas (ehp). 5 strains of e. histolytica and 2 of e. invadens were quickly and easily adapted ... | 1988 | 2903589 |
| invasive amebiasis in naturally infected new world and old world monkeys with and without clinical disease. | histopathological preparations of cecum and colon from monkeys naturally infected with invasive entamoeba histolytica were examined to determine the distribution of amebae in the tissues and the types of lesions, if any, associated with them. infections were studied in 3 new world species (10 callicebus moloch, 1 c. torquatus, and 2 aotus trivirgatus) and 3 old world species (8 macaca mulatta, 6 erythrocebus patas, and 1 cercopithecus aethiops). amebiasis was recorded as the principal or a contr ... | 1988 | 2903689 |
| entamoeba histolytica: role of amebic proteinases and polymorphonuclear leukocytes in acute experimental amebiasis in the rat. | the injection of 1 x 10(6) trophozoites of axenically grown entamoeba histolytica strain hm-1 in the subcutaneous tissue of the rat results in an acute and self-limited inflammatory process, characterized by the early onset of conspicuous tissue necrosis and focal hemorrhage in the vicinity of the parasites, followed by infiltration with polymorphonuclear leukocytes. the process develops for 5-10 hr but during that period amebic trophozoites progressively disappear, leukocytes undergo degenerati ... | 1988 | 2903831 |
| development of inflammation and augmented chemotactic responsiveness of murine peritoneal macrophages following treatment with entamoeba histolytica trophozoites. | the accumulation of inflammatory cells in the peritoneal cavity of c57bl/6 mice was examined following intraperitoneal injection of entamoeba histolytica trophozoites. two different strains of e. histolytica were used: a virulent strain (ip:0682:1) and a non-virulent strain (dkb). injection of 10(6) trophozoites of either strain resulted in significant increases in the numbers of total peritoneal cells, macrophages and polymorphonuclear cells as compared to either saline-injected control mice or ... | 1987 | 2904110 |
| amebic liver abscess and circulating immune complexes of entamoeba histolytica proteins. | an enzyme-linked immunosorbent assay (elisa) has been developed to detect amebic antigen in polyethylene-glycol-precipitable circulating immune complexes. seventy-nine percent of 191 patients with amebic liver abscesses and 46% of 26 entamoeba histolytica cyst passers had positive tests. none of the samples from 43 apparently healthy controls, 8 patients with past amebic liver abscesses or 31 patients with giardia lamblia infections were positive. one patient out of 32 with viral hepatitis and 1 ... | 1988 | 2904229 |
| a simple & rapid dot-elisa dipstick technique for detection of antibodies to entamoeba histolytica in amoebic liver abscess. | | 1988 | 2904412 |
| maintenance of integrity, viability, and adhesion of entamoeba histolytica trophozoites in different incubation media. | we have determined the integrity, viability and adhesion of entamoeba histolytica hk9 and hm1 trophozoites during their incubation in two basal culture media (tp and tyi) and three saline media ("maintenance medium" mm-1 and two others buffered with hepes). in basal culture media, more than 70% of the trophozoites maintained their integrity and adhesion to human red blood cells (rbc) for up to 4 h, and the proportion of those excluding trypan blue decreased slowly after 2 h. in saline media, the ... | 1988 | 2904497 |
| [synthesis and antiparasitic activity of arylproprenones in the nitro-5-imidazole series]. | the synthesis of new arylpropenones derived from 1-methyl-5-nitro imidazole is described. the investigation of some antiprotozoal properties has shown that compounds with the groups 4-hydroxyphenyl, 4-chlorophenyl or thiophenyl have trichomonacidal activity similar to that of metronidazole. | 1988 | 2904887 |
| clinical, microbiological and antibiotic susceptibility patterns of diarrhoea in korem, ethiopia. | two hundred patients with diarrhoea in a rehabilitation camp in ethiopia were studied in october 1985 to determine the presence of pathogens in the stool and their susceptibility to antibiotics. a total of 42 (21.1%) patients had a positive culture with enterobacteriaceae, the isolation rate was 15.6% for escherichia coli, 3.5% for shigella spp. and 2.01% for salmonella spp. in-vitro antibiotic resistance was frequent among the 42 isolates: 53% of e. coli strains were found to be resistant to am ... | 1988 | 2905012 |
| [mexican contributions to vaccines]. | | 1988 | 2905313 |
| [breast-feeding and intestinal parasites]. | the extent of intestinal parasitic infection was determined using 4 methods of fecal examination in 400 gabonese infants aged 0.5 to 24 months (86 exclusively and 151 partially breast-fed and 163 artificially fed) and in 170 of their mothers in an african urban community. parasitic prevalence was the same in the stools of the mothers from the 3 infant groups, providing evidence for the same level of exposure to parasites in all infants. exclusively and partially breast-fed infants excreted paras ... | 1988 | 2905884 |
| meleney's synergistic gangrene. | | 1988 | 2905915 |
| entamoeba histolytica infection in a rehabilitation center for mentally retarded persons in japan. | | 1988 | 2906172 |
| [intracellular location of vacuoles with acid phosphatase activity in trophozoites of entamoeba histolytica adhered to guinea pig cecal epithelium]. | | 1988 | 2906533 |
| [seroepidemiological study on amoebiasis]. | | 1988 | 2906569 |
| modulation of pathogenicity of entamoeba histolytica by histamine. | | 1988 | 2906629 |
| role of beta-glucuronidase, a lysosomal enzyme, in the pathogenesis of intestinal amoebiasis: an experimental study. | the role of beta-glucuronidase (bg), released by blood monocytes and kupffer cells, in the pathogenesis of intestinal amoebiasis was studied. guinea-pigs, infected intracaecally with entamoeba histolytica, were killed 0, 3, 7, 14 and 21 d after infection. enhanced levels of bg were observed in the cell lysates as well as supernatants of both blood monocytes and kupffer cells in the infected animals, from the 3rd day after infection, as compared to those in controls. the rise in bg levels was mor ... | 1988 | 2906765 |
| abdominal vein distension in amoebic liver abscess. | | 1988 | 2906766 |
| distribution of pathogenic zymodemes of entamoeba histolytica. | | 1988 | 2906767 |
| analysis of soluble entamoeba histolytica antigen using enzyme-l inked electroimmunotransfer blots. | soluble antigens of ten strains of e. histolytica were studied by sodium dodecylsulphate-polyacrylamide gel electrophoresis (sds-page) and enzyme-linked electroimmunotransfer blots (eitb). no relations of immune replicas to virulence, geographical origin and method of cultivation (xenic or axenic culture) were found. antigens of all ten strains tested precipitated with anti-e. histolytica human serum in the area of 30-43 kd. antigen of hk-9 strain created in this area a characteristic pattern wi ... | 1988 | 2906894 |
| immunosuppression and splenomegaly in entamoeba histolytica infection in mice. | intestinal amoebiasis caused by entamoeba histolytica trophozoites in mice is accompanied by a depression in the ability of this host to develop an immune response to sheep red blood cells. the number of splenic plaque-forming cells was reduced in mice inoculated intracecally with 2.5 x 10(5) trophozoites at 15, 25, 40, 65 and 75 days after infection when compared with non-infected mice. it was found that there was no significant difference between the spleen weight of the infected and non-infec ... | 1987 | 2907086 |
| [analysis of parasitic zymodemes]. | | 1988 | 2907394 |
| effect of histamine and serotonin on phagocytic activity of entamoeba histolytica. | | 1988 | 2907510 |
| application of a micro enzyme-linked immunosorbent assay (elisa) to detection of anti-amebic antibody in various forms of amebic infection. | techniques of a micro enzyme-linked immunosorbent assay (elisa) used for the serodiagnosis of schistosomiasis were applied to amebic infection. test sera were divided primary on the basis of serologic diagnosis and stool examination as follows; (i) gel diffusion precipitin test (gdp) positive and stool examination positive: 9 specimens, (ii) gdp positive and stool examination negative: 29 specimens, (iii) gdp negative and stool examination positive: 32 specimens. virtually all of the individuals ... | 1988 | 2907591 |
| activation of macrophages by entamoeba histolytica extracts in mice. | the effect of entamoeba histolytica extracts on the production of inflammatory macrophages and the release of hydrogen peroxide (h2o2) and superoxide (o2-) from these cells was examined in c57bl/6 mice. two different strains of e. histolytica, either virulent (ip:0682:1) or nonvirulent (dkb), were used in this study. the number of macrophages recovered from the peritoneal cavity of mice treated 5 days previously with 150 micrograms of either strain of amoebic extracts was significantly higher th ... | 1988 | 2907598 |
| action of cholesterol on virulence related biochemical functions of entamoeba histolytica. | | 1988 | 2907902 |
| long shelf-life of antigen-sensitised erythrocytes by double aldehyde stabilisation for the serodiagnosis of amoebiasis by indirect haemagglutination. | | 1988 | 2908043 |
| use of microdilution to assess in vitro antiamoebic activities of brucea javanica fruits, simarouba amara stem, and a number of quassinoids. | a microdilution technique for the assessment of in vitro activity against entamoeba histolytica was devised and validated with metronidazole. the test was used to detect the antiamoebic activities of plant extracts prepared from the traditional remedies brucea javanica fruits and simarouba amara stems. the activity was associated with quassinoid-containing fractions. the 50% inhibitory concentrations for some quassinoids against amoebae were determined by using the microdilution method. these co ... | 1988 | 2908094 |
| [studies on surface electricity of entamoeba histolytica]. | | 1988 | 2908101 |
| biological evidence of genetic exchange in entamoeba histolytica. | the demonstration of a new zymodeme of entamoeba histolytica produced in culture from cloned isolates suggested possible genetic exchange in this parasite. we have attempted to substantiate that finding by using rats as biological hosts. clones were made from 3 separate isolates of e. histolytica, each established in culture from liver pus or faeces. after enzyme characterization these clones, of zymodemes ii, xiv and xix, were paired in each of the 3 possible combinations and the mixtures injec ... | 1988 | 2908287 |
| intestinal lumen and mucosal microclimate h+ and nh3 concentrations as factors in the etiology of experimental amebiasis. | axenically cultivated entamoeba histolytica, trophozoites, strains hm-1:imss and hm-38, were suspended in solutions of nacl, 330 mosm, of varying ph and ammonium concentrations. short-term viability was inversely proportional to the ammonia concentration of the medium and was independent of the ammonium concentration and ph. the nh3-induced ameba killing was associated with a cellular alkalosis and cell swelling. while short-term trophozoite viability was unaffected by changes in the medium ph o ... | 1988 | 2908580 |
| [the incidence of amebiasis in abortion]. | | 1988 | 2908694 |
| mebendazole retention enema for severe trichuris trichiura (whipworm) infection: a case report. | the authors report a case of massive trichuris trichiura infection, resulting in severe anaemia and congestive cardiac failure in a 9-year-old iban boy, who was resistant to the usual oral anthelmintic treatment, but promptly responded to mebendazole retention enema. this patient also had an associated entamoeba histolytica infection. | 1989 | 2918577 |
| ameboma presenting as acute urinary retention. | a patient with several weeks of diarrhea developed for the first time severe urinary frequency and urgency and was admitted with acute urinary retention. imaging techniques showed a large mass compressing the posterior wall of the bladder adjacent to the rectosigmoid colon, leading to a bilateral dilatation of the ureters. cystoscopy and biopsy of the affected part of the bladder showed no tumor but a nonspecific acute inflammation. after identification of trophozoites of entamoeba histolytica i ... | 1989 | 2929570 |
| comparison between the efficacy of a single dose of secnidazole with a 5-day course of tetracycline and clioquinol in the treatment of acute intestinal amoebiasis. | a study was carried out in 80 patients with acute intestinal amoebiasis to compare the efficacy of treatment with a single oral dose of 2 g secnidazole and a 5-day course of 750 mg tetracycline plus 1 g clioquinol per day. patients were allocated at random into one or other treatment group and returned for clinical assessment and microscopic examination of stools for the presence of the trophozoite or haematophagous form of entamoeba histolytica on days 1 to 7, 14, 21 and 28. the results showed ... | 1985 | 2932742 |
| intestinal helminthiasis and haemoparasitism in an area of endemic dracunculiasis in oyo state of nigeria. | in the course of a survey of human intestinal and haemoparasites in oluyole local government area of nigeria known to be endemic for dracunculiasis, most of the inhabitants were burdened with parasitic infections which include ascariasis (43.7%), hookworm infection (27.1%), trichuriasis (31%), strongyloidiasis (2.5%), entamoeba histolytica infestation (3.9%), e. coli infestation (2.7%) and plasmodiasis (43.7%). peak of ascariasis was among the 1-10 years age group while those of trichuriasis, ho ... | 1986 | 2944853 |
| the effect of splenectomy on resistance of mice to entamoeba histolytica infection. | the role of the spleen in amoebic infection was examined in mice, using strains selected as being either genetically-susceptible (c57bl/6) or genetically-resistant (a/j) to amoebiasis. splenectomized and sham-operated animals were inoculated intracaecally with 2.5 x 10(5) polyxenic trophozoites of e. histolytica at 6, 12 and 15 days post-splenectomy. the animals were killed 6 or 12 days after infection and the parasite burden was evaluated. removal of the spleen in both susceptible and resistant ... | 1985 | 2999680 |
| [epidemiology, etiology and laboratory diagnosis of infectious diarrhea diseases in the tropics]. | diarrhoeal diseases belong to the leading causes of morbidity and mortality in tropical countries, especially in infants and small children. about one billion episodes are estimated for this group of age with 4.6 million fatalities. many causes are discussed to explain the high incidence: bottle feeding of infants, protein malnutrition, unsafe drinking water and unsafe disposal of excrements and sewage, unsufficient consciousness of personal and domestic hygiene, lack of knowledge on the origin ... | 1985 | 3000920 |
| pathogens that cause travelers' diarrhea in latin america and africa. | with the advent of rapid and convenient means of transportation, millions of persons travel each year from industrialized to developing countries in the tropics and subtropics. these travelers are at risk for a variety of infectious diseases that are endemic in these areas; the most frequently occurring of these is diarrhea. studies of groups of travelers to latin america and africa have found that approximately one-half develop diarrhea during their stay abroad. etiologic investigations of thes ... | 1986 | 3014633 |
| two-year survey of etiologic agents of diarrheal disease at san lazaro hospital, manila, republic of the philippines. | the prevalence of bacterial pathogens and rotavirus in 2,908 patients with diarrhea who were admitted to san lazaro hospital in manila in 1983 and 1984 was determined. one or more enteric pathogens were isolated or detected in samples from 1,698 (58.4%) patients. isolation rates for the various enteropathogens were as follows: rotavirus, 30.6%; shigella spp., 11.6%; salmonella spp., 9.2%; enterotoxigenic escherichia coli (1983 only), 7.8%; vibrio cholerae biotype eltor, 3.8%; non-o1 v. cholerae, ... | 1987 | 3038946 |
| parasitic pneumonia. | with few exceptions, the parasitic pneumonias most commonly encountered in the western hemisphere are diseases of compromised hosts; patients with aids are at particular risk. pneumocystis carinii pneumonia occurs eventually in 80% of aids patients; bronchoalveolar lavage is quite sensitive in establishing this diagnosis. toxoplasma gondii pneumonia, seen most often in the patient with aids, is characterized by multisystem involvement. strongyloides stercoralis infection is endemic in the southe ... | 1988 | 3041517 |
| aetiologic agents of diarrhoeal diseases in hospitalised children in rawalpindi, pakistan. | the causes of diarrhoea were diagnosed in 250 children and 250 controls, a sample of 3,500 children who reported to the rawalpindi general hospital, pakistan between may 1983 and april 1984. the pathogens identified in children with diarrhoea were enteropathogenic escherichia coli (epec) (43.2%), enterotoxigenic e. coli (14%), rotavirus (9.6%), shigella spp (3.6%), salmonella spp (3.2%) and giardia lamblia (1.2%). mixed infections were found in 2.4% of patients. the following pathogens were dete ... | 1988 | 3077947 |